MD00G1060800.v1.1
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002586
Physical Location & Seq
Forward (+)
11235704 .. 11237428
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1060800.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGCAAAACTCGTAGGATTTCTGGTTTTCTTCACAGCCTTCGTGGTTGCAGCAGCAGATTGGAACATTTTGAAAACCCGACAAACCGATAACGTTGAATTGGGAGATGAGTTGAAGAAGTACTGTGAGAGTTGGAGGATCAATGTTGAAGTGAACAACATTAGAGGGTTTGAGGTTGTACCCCAAGAATGTGTTTGGTACGTTAAGAAATACATGACATCGTCTCAGTACAAGGCTGACTCGCAGAAAGCCTTGGAGGAGGTCACTCTCTACTTGAGCAGCTGCTGCAAACTGGAGGGTGATGGCAAAAATGCTTGGATTTTTGACGTTGATGATACACTTCTCTCCACCATCCCTTACTTCAAGAAACACGGGTTCGGGGGAGAAAAGCTGAATGGGACTTGTTTGGAGGCATGGATGAGAGAAGGCAAGGCACCAGCTCTTGAGCACACACTGAAGCTCTTCCATGAGATTAAAGATAGAGGGATCAAGATCTTTTTAATCTCTTCAAGAAGGGAAGATTTGAGATCATCAAGTGTGGATAATCTCATTAGGGTTGGATACCATGGATGGACTGGTTTTATATTAAGGGGTCTTGAAGATGAGTTCAAGGAAGTGCAGAAATACAAGTCCGATGCAAGGCGTAGACTGATTGATGAAGGTTACCGCATTTGGGGAATTATTGGAGACCAGTGGAGCAGTTTTGAGGGGCTTCCCATGGCAAAAAGGACATTCAAATTACCCAATTCCATGTACTATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

255

Amino Acids

29.46

Weight (kDa)

8.16

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 38 - 254 5e-77 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 646
AciI CCGC 1 cut(s) 667
AclI AACGTT 1 cut(s) 93
AclWI GGATC 2 cut(s) 146, 494
AcuI CTGAAG 1 cut(s) 476
AfaI GTAC 5 cut(s) 122, 180, 200, 230, 755
AfiI CCNNNNNNNGG 2 cut(s) 639, 673
AgsI TTSAA 9 cut(s) 73, 98, 115, 149, 364, 510, 599, 610, 736
AluBI AGCT 4 cut(s) 282, 391, 440, 460
AluI AGCT 4 cut(s) 282, 391, 440, 460
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 2 cut(s) 228, 681
AlwI GGATC 2 cut(s) 146, 494
AlwNI CAGNNNCTG 1 cut(s) 285
ApeKI GCWGC 5 cut(s) 50, 53, 279, 282, 285
Asp700I GAANNNNTTC 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 5 cut(s) 62, 65, 269, 272, 291
BccI CCATC 3 cut(s) 296, 359, 564
BciVI GTATCC 1 cut(s) 554
BcoDI GTCTC 2 cut(s) 228, 681
BfaI CTAG 1 cut(s) 763
BfuI GTATCC 1 cut(s) 554
BglII AGATCT 1 cut(s) 492
BisI GCNGC 5 cut(s) 51, 54, 280, 283, 286
BlsI GCNGC 5 cut(s) 52, 55, 281, 284, 287
BmcAI AGTACT 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 435
BmsI GCATC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 295, 464
BsaBI GATNNNNATC 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 681
BsaJI CCNNGG 3 cut(s) 252, 565, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 639, 673
Bse1I ACTGG 3 cut(s) 297, 580, 691
Bse8I GATNNNNATC 1 cut(s) 491
BseDI CCNNGG 3 cut(s) 252, 565, 717
BseGI GGATG 3 cut(s) 351, 423, 575
BseJI GATNNNNATC 1 cut(s) 491
BseLI CCNNNNNNNGG 2 cut(s) 639, 673
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 3 cut(s) 297, 580, 691
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 5 cut(s) 62, 65, 269, 272, 291
BsgI GTGCAG 1 cut(s) 638
BshNI GGYRCC 1 cut(s) 433
BsiHKAI GWGCWC 1 cut(s) 450
BslFI GGGAC 1 cut(s) 412
BslI CCNNNNNNNGG 2 cut(s) 639, 673
BsmAI GTCTC 2 cut(s) 228, 681
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 412
Bso31I GGTCTC 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 4 cut(s) 138, 486, 492, 527
Bsp19I CCATGG 2 cut(s) 565, 717
BspACI CCGC 1 cut(s) 667
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 2 cut(s) 146, 494
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 681
BsrI ACTGG 3 cut(s) 297, 580, 691
BssECI CCNNGG 3 cut(s) 252, 565, 717
BssMI GATC 4 cut(s) 138, 486, 492, 527
BssT1I CCWWGG 3 cut(s) 252, 565, 717
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 2 cut(s) 467, 511
BstAPI GCANNNNNTGC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 225
BstDSI CCRYGG 2 cut(s) 565, 717
BstEII GGTNACC 1 cut(s) 662
BstF5I GGATG 3 cut(s) 351, 423, 575
BstKTI GATC 4 cut(s) 141, 489, 495, 530
BstMAI GTCTC 2 cut(s) 228, 681
BstMBI GATC 4 cut(s) 138, 486, 492, 527
BstMWI GCNNNNNNNGC 1 cut(s) 285
BstPI GGTNACC 1 cut(s) 662
BstV1I GCAGC 5 cut(s) 62, 65, 269, 272, 291
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BsuI GTATCC 1 cut(s) 554
BtgI CCRYGG 2 cut(s) 565, 717
BtsCI GGATG 3 cut(s) 351, 423, 575
BtsIMutI CAGTG 2 cut(s) 452, 698
CaiI CAGNNNCTG 1 cut(s) 285
Csp6I GTAC 5 cut(s) 121, 179, 199, 229, 754
CviAII CATG 6 cut(s) 214, 414, 467, 566, 718, 751
CviJI RGCY 8 cut(s) 38, 236, 251, 282, 391, 440, 460, 712
CviKI_1 RGCY 8 cut(s) 38, 236, 251, 282, 391, 440, 460, 712
CviQI GTAC 5 cut(s) 121, 179, 199, 229, 754
DdeI CTNAG 1 cut(s) 225
DpnI GATC 4 cut(s) 140, 488, 494, 529
DpnII GATC 4 cut(s) 138, 486, 492, 527
Eam1104I CTCTTC 2 cut(s) 467, 511
EarI CTCTTC 2 cut(s) 467, 511
Eco130I CCWWGG 3 cut(s) 252, 565, 717
Eco31I GGTCTC 1 cut(s) 681
Eco57I CTGAAG 1 cut(s) 476
Eco91I GGTNACC 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 662
EcoT14I CCWWGG 3 cut(s) 252, 565, 717
ErhI CCWWGG 3 cut(s) 252, 565, 717
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 6 cut(s) 217, 417, 470, 569, 721, 754
FaiI YATR 8 cut(s) 215, 415, 468, 567, 584, 719, 752, 759
FaqI GGGAC 1 cut(s) 412
FatI CATG 6 cut(s) 213, 413, 466, 565, 717, 750
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 5 cut(s) 51, 54, 280, 283, 286
FokI GGATG 3 cut(s) 338, 430, 582
Fsp4HI GCNGC 5 cut(s) 51, 54, 280, 283, 286
FspBI CTAG 1 cut(s) 763
GluI GCNGC 5 cut(s) 51, 54, 280, 283, 286
GsuI CTGGAG 1 cut(s) 314
Hin1II CATG 6 cut(s) 217, 417, 470, 569, 721, 754
HinfI GANTC 1 cut(s) 239
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 154, 647
Hpy188I TCNGA 1 cut(s) 634
Hpy188III TCNNGA 5 cut(s) 364, 443, 490, 510, 596
Hpy8I GTNNAC 2 cut(s) 154, 647
HpyAV CCTTC 4 cut(s) 49, 419, 507, 653
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 3 cut(s) 93, 201, 327
HpyCH4V TGCA 4 cut(s) 50, 288, 619, 638
HpyF10VI GCNNNNNNNGC 1 cut(s) 285
HpyF3I CTNAG 1 cut(s) 225
HpySE526I ACGT 3 cut(s) 93, 201, 327
Hsp92II CATG 6 cut(s) 217, 417, 470, 569, 721, 754
Kzo9I GATC 4 cut(s) 138, 486, 492, 527
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 1 cut(s) 696
LpnPI CCDG 5 cut(s) 8, 278, 450, 561, 704
Lsp1109I GCAGC 5 cut(s) 62, 65, 269, 272, 291
LweI GCATC 1 cut(s) 625
MaeI CTAG 1 cut(s) 763
MaeII ACGT 3 cut(s) 93, 201, 327
MaeIII GTNAC 2 cut(s) 262, 662
MalI GATC 4 cut(s) 140, 488, 494, 529
MboI GATC 4 cut(s) 138, 486, 492, 527
MboII GAAGA 6 cut(s) 22, 127, 454, 498, 530, 611
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 4 cut(s) 98, 678, 737, 745
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 2 cut(s) 113, 538
MnlI CCTC 9 cut(s) 129, 158, 166, 250, 253, 289, 403, 476, 700
MroXI GAANNNNTTC 1 cut(s) 461
MseI TTAA 4 cut(s) 204, 474, 500, 587
MspA1I CMGCKG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 285
NcoI CCATGG 2 cut(s) 565, 717
NdeII GATC 4 cut(s) 138, 486, 492, 527
NlaIII CATG 6 cut(s) 217, 417, 470, 569, 721, 754
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 262
PciSI GCTCTTC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 461
PflFI GACNNNGTC 1 cut(s) 220
PkrI GCNGC 5 cut(s) 52, 55, 281, 284, 287
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
Psp1406I AACGTT 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 662
PspN4I GGNNCC 1 cut(s) 435
PstNI CAGNNNCTG 1 cut(s) 285
PsuI RGATCY 1 cut(s) 492
PsyI GACNNNGTC 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 282
RsaI GTAC 5 cut(s) 122, 180, 200, 230, 755
RsaNI GTAC 5 cut(s) 121, 179, 199, 229, 754
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 4 cut(s) 204, 474, 500, 587
SatI GCNGC 5 cut(s) 51, 54, 280, 283, 286
Sau3AI GATC 4 cut(s) 138, 486, 492, 527
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 1 cut(s) 233
SduI GDGCHC 1 cut(s) 450
SfaNI GCATC 1 cut(s) 625
SmlI CTYRAG 2 cut(s) 274, 443
SmoI CTYRAG 2 cut(s) 274, 443
Sse9I AATT 4 cut(s) 98, 678, 737, 745
SsiI CCGC 1 cut(s) 667
SspMI CTAG 1 cut(s) 763
StyI CCWWGG 3 cut(s) 252, 565, 717
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 3 cut(s) 96, 204, 330
TasI AATT 4 cut(s) 98, 678, 737, 745
TatI WGTACW 3 cut(s) 120, 228, 753
Tru1I TTAA 4 cut(s) 204, 474, 500, 587
Tru9I TTAA 4 cut(s) 204, 474, 500, 587
TscAI CASTG 2 cut(s) 459, 698
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 5 cut(s) 50, 53, 279, 282, 285
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 672
TspRI CASTG 2 cut(s) 459, 698
Tth111I GACNNNGTC 1 cut(s) 220
XcmI CCANNNNNNNNNTGG 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 646
XmnI GAANNNNTTC 1 cut(s) 461
XspI CTAG 1 cut(s) 763
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.