FvH4_2g21010

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005522
Physical Location & Seq
Forward (+)
17509792 .. 17510759
968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21010.t1

Sequence Viewer

Length: 816 bp
ATGGGGATTCCATGTAGCAGTCAGAAGCTTTTTCGTGAAGGGCAGTTGCTTGATCATGAGGCCGTATCGCTAAAGACATTCAACAACAAGATGGAAGCTGAATCTAGTGTTATTTATGTGACTTTCGGGGATGGAGATGATGATCAAGTCCACGAAGCTGAACATATAAGCTTCATATTTGAATTTGAGAGAGAAGATATGATGCGGCTTGAATTCAAGGTCAGCATATCTGATGAAAGCAGCAATCCTCTAGTCTCTTTGTTCAAAATCAAGTTGAGATCATTGTGCACAATTTTGGATCTCAAGGCAGCAATCCAGAGCTTAACCAACTGTCGAATTTGTAACCAAAAGCTATGCTGCGATGATCTAATTCTGAAAGATTGGGAAACATTGGTGACCTGCAACATTACAAAGTGGTACATTGAAGGCCGCTTTCAGCTGACACCCATTTGGGACACTGACAAGATATTTTCTGCAGACAAAACCCTTTATGAAGTTTTGGATAGCATGCCAGAAAAGAGGCTGGAAAGCATCGGCAGCCAGATAAATGTAACAGGGCCGAATGGCGCAACCACTAAGGTGCATGTGAACCCTTCTTACACATTCAAGGATTTAAAGGAGAAGATTGAACTGAATTGTGGAATCCCATATGGTGTACAAAGACTTACTGAAGATGGTGCTGAACTTAATGATGATCTGACTATAAGGTTATATGAGGAATATTACGGCAGTCTGTGGAAATTAAGGTGGAACTTTTGTGGTACTTCCTGGTCATTCTTCTACCCATCGTACACGGATTGCAGCAGGATTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

272

Amino Acids

31.21

Weight (kDa)

4.84

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 182 - 238 7.3e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 407
AciI CCGC 2 cut(s) 205, 430
AclWI GGATC 1 cut(s) 306
AcsI RAATTY 3 cut(s) 182, 212, 336
AcuI CTGAAG 1 cut(s) 690
AfaI GTAC 4 cut(s) 419, 657, 763, 791
AgsI TTSAA 8 cut(s) 82, 182, 212, 217, 265, 425, 607, 629
AjnI CCWGG 1 cut(s) 767
AleI CACNNNNGTG 1 cut(s) 578
AluBI AGCT 7 cut(s) 28, 98, 158, 171, 321, 352, 439
AluI AGCT 7 cut(s) 28, 98, 158, 171, 321, 352, 439
Alw21I GWGCWC 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 259
Alw44I GTGCAC 1 cut(s) 286
AlwI GGATC 1 cut(s) 306
AoxI GGCC 3 cut(s) 60, 427, 557
ApaLI GTGCAC 1 cut(s) 286
ApeKI GCWGC 5 cut(s) 240, 308, 357, 537, 801
ApoI RAATTY 3 cut(s) 182, 212, 336
AspLEI GCGC 1 cut(s) 569
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 290
BbvI GCAGC 4 cut(s) 252, 320, 344, 549
BccI CCATC 4 cut(s) 85, 125, 668, 793
BceAI ACGGC 2 cut(s) 47, 742
BciT130I CCWGG 1 cut(s) 769
BclI TGATCA 2 cut(s) 52, 142
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 3 cut(s) 105, 251, 814
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 407
BisI GCNGC 7 cut(s) 206, 241, 309, 358, 430, 538, 802
BlsI GCNGC 7 cut(s) 207, 242, 310, 359, 431, 539, 803
Bme1390I CCNGG 1 cut(s) 769
BmgT120I GGNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 769
BmsI GCATC 2 cut(s) 192, 540
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 1 cut(s) 769
BseGI GGATG 1 cut(s) 136
BseJI GATNNNNATC 1 cut(s) 141
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 4 cut(s) 252, 320, 344, 549
BshFI GGCC 3 cut(s) 62, 429, 559
BsiHKAI GWGCWC 1 cut(s) 290
BslFI GGGAC 1 cut(s) 467
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 3 cut(s) 62, 429, 559
Bsp1286I GDGCHC 1 cut(s) 290
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 6 cut(s) 52, 142, 278, 298, 364, 694
BspACI CCGC 2 cut(s) 205, 430
BspANI GGCC 3 cut(s) 62, 429, 559
BspHI TCATGA 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 655
BssMI GATC 6 cut(s) 52, 142, 278, 298, 364, 694
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 655
BstC8I GCNNGC 1 cut(s) 509
BstDEI CTNAG 1 cut(s) 576
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 1 cut(s) 136
BstHHI GCGC 1 cut(s) 569
BstKTI GATC 6 cut(s) 55, 145, 281, 301, 367, 697
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 6 cut(s) 52, 142, 278, 298, 364, 694
BstMWI GCNNNNNNNGC 2 cut(s) 537, 810
BstNI CCWGG 1 cut(s) 769
BstNSI RCATGY 2 cut(s) 511, 587
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 1 cut(s) 767
BstSFI CTRYAG 1 cut(s) 474
BstSLI GKGCMC 1 cut(s) 290
BstV1I GCAGC 4 cut(s) 252, 320, 344, 549
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 3 cut(s) 62, 429, 559
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 456
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 1 cut(s) 509
CciI TCATGA 1 cut(s) 55
CfoI GCGC 1 cut(s) 569
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 4 cut(s) 418, 656, 762, 790
CviAII CATG 4 cut(s) 12, 56, 508, 584
CviQI GTAC 4 cut(s) 418, 656, 762, 790
DdeI CTNAG 1 cut(s) 576
DpnI GATC 6 cut(s) 54, 144, 280, 300, 366, 696
DpnII GATC 6 cut(s) 52, 142, 278, 298, 364, 694
DraI TTTAAA 1 cut(s) 615
Eco57I CTGAAG 1 cut(s) 690
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 767
FaeI CATG 4 cut(s) 15, 59, 511, 587
FaqI GGGAC 1 cut(s) 467
FatI CATG 4 cut(s) 11, 55, 507, 583
FauNDI CATATG 1 cut(s) 649
FbaI TGATCA 2 cut(s) 52, 142
Fnu4HI GCNGC 7 cut(s) 206, 241, 309, 358, 430, 538, 802
FokI GGATG 1 cut(s) 143
Fsp4HI GCNGC 7 cut(s) 206, 241, 309, 358, 430, 538, 802
FspBI CTAG 3 cut(s) 105, 251, 814
GlaI GCGC 1 cut(s) 568
GluI GCNGC 7 cut(s) 206, 241, 309, 358, 430, 538, 802
HaeIII GGCC 3 cut(s) 62, 429, 559
HhaI GCGC 1 cut(s) 569
Hin1II CATG 4 cut(s) 15, 59, 511, 587
Hin6I GCGC 1 cut(s) 567
HinP1I GCGC 1 cut(s) 567
HindIII AAGCTT 2 cut(s) 26, 169
HinfI GANTC 3 cut(s) 7, 101, 642
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 5 cut(s) 151, 288, 589, 656, 792
Hpy188I TCNGA 4 cut(s) 24, 232, 375, 699
Hpy188III TCNNGA 3 cut(s) 35, 56, 316
Hpy8I GTNNAC 5 cut(s) 151, 288, 589, 656, 792
HpyAV CCTTC 3 cut(s) 32, 419, 603
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4V TGCA 5 cut(s) 288, 402, 476, 583, 801
HpyF10VI GCNNNNNNNGC 2 cut(s) 537, 810
HpyF3I CTNAG 1 cut(s) 576
Hsp92II CATG 4 cut(s) 15, 59, 511, 587
HspAI GCGC 1 cut(s) 567
Ksp22I TGATCA 2 cut(s) 52, 142
Kzo9I GATC 6 cut(s) 52, 142, 278, 298, 364, 694
LpnPI CCDG 9 cut(s) 329, 412, 509, 525, 540, 554, 754, 781, 790
Lsp1109I GCAGC 4 cut(s) 252, 320, 344, 549
LweI GCATC 2 cut(s) 192, 540
MaeI CTAG 3 cut(s) 105, 251, 814
MaeIII GTNAC 4 cut(s) 118, 341, 394, 550
MalI GATC 6 cut(s) 54, 144, 280, 300, 366, 696
MboI GATC 6 cut(s) 52, 142, 278, 298, 364, 694
MboII GAAGA 4 cut(s) 206, 634, 683, 769
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 290
MluCI AATT 7 cut(s) 182, 212, 291, 336, 369, 634, 740
MnlI CCTC 4 cut(s) 52, 258, 513, 709
MseI TTAA 4 cut(s) 323, 614, 687, 743
MslI CAYNNNNRTG 1 cut(s) 578
MspA1I CMGCKG 1 cut(s) 439
MspR9I CCNGG 1 cut(s) 769
MvaI CCWGG 1 cut(s) 769
MwoI GCNNNNNNNGC 2 cut(s) 537, 810
NdeI CATATG 1 cut(s) 649
NdeII GATC 6 cut(s) 52, 142, 278, 298, 364, 694
NlaIII CATG 4 cut(s) 15, 59, 511, 587
NmuCI GTSAC 2 cut(s) 118, 394
NspI RCATGY 2 cut(s) 511, 587
OliI CACNNNNGTG 1 cut(s) 578
PaeI GCATGC 1 cut(s) 511
PagI TCATGA 1 cut(s) 55
PfeI GAWTC 3 cut(s) 7, 101, 642
PkrI GCNGC 7 cut(s) 207, 242, 310, 359, 431, 539, 803
Psp6I CCWGG 1 cut(s) 767
PspEI GGTNACC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 767
PspPI GGNCC 1 cut(s) 557
PsrI GAACNNNNNNTAC 2 cut(s) 581, 613
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 439
RsaI GTAC 4 cut(s) 419, 657, 763, 791
RsaNI GTAC 4 cut(s) 418, 656, 762, 790
RseI CAYNNNNRTG 1 cut(s) 578
SaqAI TTAA 4 cut(s) 323, 614, 687, 743
SatI GCNGC 7 cut(s) 206, 241, 309, 358, 430, 538, 802
Sau3AI GATC 6 cut(s) 52, 142, 278, 298, 364, 694
Sau96I GGNCC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 769
SduI GDGCHC 1 cut(s) 290
SfaNI GCATC 2 cut(s) 192, 540
SfcI CTRYAG 1 cut(s) 474
SmiMI CAYNNNNRTG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
SphI GCATGC 1 cut(s) 511
Sse9I AATT 7 cut(s) 182, 212, 291, 336, 369, 634, 740
SsiI CCGC 2 cut(s) 205, 430
SspI AATATT 1 cut(s) 722
SspMI CTAG 3 cut(s) 105, 251, 814
StyD4I CCNGG 1 cut(s) 767
TaaI ACNGT 1 cut(s) 332
TaqI TCGA 1 cut(s) 334
TasI AATT 7 cut(s) 182, 212, 291, 336, 369, 634, 740
TatI WGTACW 1 cut(s) 655
TauI GCSGC 2 cut(s) 208, 432
TfiI GAWTC 3 cut(s) 7, 101, 642
Tru1I TTAA 4 cut(s) 323, 614, 687, 743
Tru9I TTAA 4 cut(s) 323, 614, 687, 743
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 118, 394
TseI GCWGC 5 cut(s) 240, 308, 357, 537, 801
Tsp45I GTSAC 2 cut(s) 118, 394
TspDTI ATGAA 3 cut(s) 163, 249, 507
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 1 cut(s) 463
VneI GTGCAC 1 cut(s) 286
XapI RAATTY 3 cut(s) 182, 212, 336
XceI RCATGY 2 cut(s) 511, 587
XspI CTAG 3 cut(s) 105, 251, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.