Rroxscaffold_7G00181470

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005522
Physical Location & Seq
Reverse (-)
20566829 .. 20569169
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181470.1

Sequence Viewer

Length: 621 bp
ATGGTCCAATTACTAACTGCTCCTTTGGGCTTGGGCATTGTTTGTGGGCTCGTTATGGCTGTAGTTTGGGTTGGGGCTCAATTTTTGGGCCATGCCCTAGGCAATGAAGAGGTCACCCTCTTCACTCTAAACAGCTCCGACTCCATTTCCCACCTCAAGACCCTCATCAAAATCAAAGACGGCGTCCCCAGAAAGCGCCAGTTCCTAATCCACAACGGCAAATTGCTCCATGACCACGAAACCCTTTCGGCTTCCGGCCTGGAAAGCTGGTCCGTTGTCTATGCCATTTTCTGCCTGGGGGAGATCATCTGTCCCAAAATGGATGCTGCCCACGTGTTTGTGAAAATGCCAGAGTGGGCTGAACTGGTGCCGACGAAGGTGCAGCCGTGGTATACCGGGAGAGAAGTCAAGGCGTTGATGGAGAGTGCTACGGGGATGTTTGTTGACGAGTCTGTGGTGAGTCATGGAGGTAAGATCATAGACGATTCCGTGAGGTTTTGTAATTGTGATGTGAATGAGGAAATGCCGATTTATTTACTGTTGAGAGGGAAGAAATTTCGGATTGCTGCGAAAGGTTGTGGGTGGGAAGAATTGATTGTGGTGTGTAAAGAAGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

206

Amino Acids

22.74

Weight (kDa)

5.97

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 35 - 93 3.1e-11 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 367
AccI GTMKAC 1 cut(s) 392
AcsI RAATTY 1 cut(s) 554
AcvI CACGTG 1 cut(s) 334
AcyI GRCGYC 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 333
AjnI CCWGG 2 cut(s) 258, 294
AluBI AGCT 2 cut(s) 135, 267
AluI AGCT 2 cut(s) 135, 267
AoxI GGCC 2 cut(s) 88, 256
ApeKI GCWGC 3 cut(s) 326, 382, 566
ApoI RAATTY 1 cut(s) 554
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AspA2I CCTAGG 1 cut(s) 97
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 3 cut(s) 4, 88, 270
AsuC2I CCSGG 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 106, 469
AvaII GGWCC 2 cut(s) 4, 270
AvrII CCTAGG 1 cut(s) 97
BanI GGYRCC 1 cut(s) 367
BanII GRGCYC 2 cut(s) 51, 79
BbrPI CACGTG 1 cut(s) 334
BbvI GCAGC 3 cut(s) 313, 394, 553
BccI CCATC 2 cut(s) 412, 608
BceAI ACGGC 3 cut(s) 196, 232, 370
BcgI CGANNNNNNTGC 2 cut(s) 361, 395
BciT130I CCWGG 2 cut(s) 260, 296
BcnI CCSGG 1 cut(s) 397
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 60
BfoI RGCGCY 1 cut(s) 199
BisI GCNGC 3 cut(s) 327, 383, 567
BlnI CCTAGG 1 cut(s) 97
BlsI GCNGC 3 cut(s) 328, 384, 568
Bme1390I CCNGG 3 cut(s) 260, 296, 397
Bme18I GGWCC 2 cut(s) 4, 270
BmgT120I GGNCC 3 cut(s) 4, 88, 270
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 3 cut(s) 260, 296, 397
BmsI GCATC 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 397
BsaAI YACGTR 1 cut(s) 334
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 97, 295, 386
Bse1I ACTGG 2 cut(s) 199, 369
Bse3DI GCAATG 1 cut(s) 109
BseBI CCWGG 2 cut(s) 260, 296
BseDI CCNNGG 3 cut(s) 97, 295, 386
BseGI GGATG 2 cut(s) 328, 441
BseMI GCAATG 1 cut(s) 109
BseNI ACTGG 2 cut(s) 199, 369
BseXI GCAGC 3 cut(s) 313, 394, 553
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 2 cut(s) 90, 258
BshNI GGYRCC 1 cut(s) 367
BsiSI CCGG 2 cut(s) 255, 396
BslFI GGGAC 2 cut(s) 170, 297
BsmFI GGGAC 2 cut(s) 170, 297
BsnI GGCC 2 cut(s) 90, 258
Bsp1286I GDGCHC 2 cut(s) 51, 79
Bsp143I GATC 2 cut(s) 303, 474
BspANI GGCC 2 cut(s) 90, 258
BspLI GGNNCC 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 2 cut(s) 199, 369
BssECI CCNNGG 3 cut(s) 97, 295, 386
BssMI GATC 2 cut(s) 303, 474
BssNAI GTATAC 1 cut(s) 393
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 97
Bst1107I GTATAC 1 cut(s) 393
Bst2UI CCWGG 2 cut(s) 260, 296
Bst4CI ACNGT 1 cut(s) 540
Bst6I CTCTTC 2 cut(s) 102, 125
BstACI GRCGYC 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 334
BstDSI CCRYGG 1 cut(s) 386
BstEII GGTNACC 1 cut(s) 112
BstF5I GGATG 2 cut(s) 328, 441
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 306, 477
BstMBI GATC 2 cut(s) 303, 474
BstMWI GCNNNNNNNGC 1 cut(s) 264
BstNI CCWGG 2 cut(s) 260, 296
BstPI GGTNACC 1 cut(s) 112
BstSCI CCNGG 3 cut(s) 258, 294, 395
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 313, 394, 553
BstZ17I GTATAC 1 cut(s) 393
BsuRI GGCC 2 cut(s) 90, 258
BtgI CCRYGG 1 cut(s) 386
BtsCI GGATG 2 cut(s) 328, 441
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 3 cut(s) 4, 88, 270
CseI GACGC 1 cut(s) 172
CviAII CATG 3 cut(s) 92, 230, 464
DpnI GATC 2 cut(s) 305, 476
DpnII GATC 2 cut(s) 303, 474
Eam1104I CTCTTC 2 cut(s) 102, 125
EarI CTCTTC 2 cut(s) 102, 125
Eco130I CCWWGG 1 cut(s) 97
Eco24I GRGCYC 2 cut(s) 51, 79
Eco47I GGWCC 2 cut(s) 4, 270
Eco72I CACGTG 1 cut(s) 334
Eco91I GGTNACC 1 cut(s) 112
EcoO65I GGTNACC 1 cut(s) 112
EcoRII CCWGG 2 cut(s) 258, 294
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 2 cut(s) 51, 79
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 3 cut(s) 95, 233, 467
FaiI YATR 7 cut(s) 56, 93, 231, 282, 393, 465, 479
FaqI GGGAC 2 cut(s) 170, 297
FatI CATG 3 cut(s) 91, 229, 463
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 3 cut(s) 327, 383, 567
FokI GGATG 2 cut(s) 335, 448
FriOI GRGCYC 2 cut(s) 51, 79
Fsp4HI GCNGC 3 cut(s) 327, 383, 567
FspBI CTAG 1 cut(s) 98
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 327, 383, 567
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 90, 258
HapII CCGG 2 cut(s) 255, 396
HgaI GACGC 1 cut(s) 172
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 95, 233, 467
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 4 cut(s) 140, 449, 460, 485
HpaII CCGG 2 cut(s) 255, 396
HphI GGTGA 2 cut(s) 106, 469
Hpy166II GTNNAC 2 cut(s) 393, 445
Hpy188I TCNGA 2 cut(s) 139, 561
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 2 cut(s) 393, 445
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 1 cut(s) 370
HpyCH4III ACNGT 1 cut(s) 540
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 95, 233, 467
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 303, 474
LmnI GCTCC 3 cut(s) 25, 140, 231
Lsp1109I GCAGC 3 cut(s) 313, 394, 553
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 112
MalI GATC 2 cut(s) 305, 476
MboI GATC 2 cut(s) 303, 474
MboII GAAGA 4 cut(s) 112, 119, 562, 599
MhlI GDGCHC 2 cut(s) 51, 79
MluCI AATT 6 cut(s) 8, 80, 221, 502, 554, 590
MlyI GAGTC 3 cut(s) 134, 458, 469
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 8 cut(s) 103, 128, 164, 173, 461, 486, 511, 539
MseI TTAA 1 cut(s) 619
MspI CCGG 2 cut(s) 255, 396
MspR9I CCNGG 3 cut(s) 260, 296, 397
MvaI CCWGG 2 cut(s) 260, 296
MwoI GCNNNNNNNGC 1 cut(s) 264
NciI CCSGG 1 cut(s) 397
NdeII GATC 2 cut(s) 303, 474
NlaIII CATG 3 cut(s) 95, 233, 467
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 485
PflFI GACNNNGTC 1 cut(s) 182
PkrI GCNGC 3 cut(s) 328, 384, 568
PleI GAGTC 3 cut(s) 134, 457, 468
PmaCI CACGTG 1 cut(s) 334
PmlI CACGTG 1 cut(s) 334
PpsI GAGTC 3 cut(s) 134, 457, 468
Ppu21I YACGTR 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 258, 294
PspCI CACGTG 1 cut(s) 334
PspEI GGTNACC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 258, 294
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 3 cut(s) 4, 88, 270
PsyI GACNNNGTC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 619
SatI GCNGC 3 cut(s) 327, 383, 567
Sau3AI GATC 2 cut(s) 303, 474
Sau96I GGNCC 3 cut(s) 4, 88, 270
SchI GAGTC 3 cut(s) 134, 458, 469
ScrFI CCNGG 3 cut(s) 260, 296, 397
SduI GDGCHC 2 cut(s) 51, 79
SetI ASST 9 cut(s) 114, 137, 156, 269, 336, 381, 472, 497, 577
SfaNI GCATC 1 cut(s) 313
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 2 cut(s) 4, 270
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 6 cut(s) 8, 80, 221, 502, 554, 590
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 258, 294, 395
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 1 cut(s) 540
TaiI ACGT 1 cut(s) 336
TasI AATT 6 cut(s) 8, 80, 221, 502, 554, 590
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 1 cut(s) 619
Tru9I TTAA 1 cut(s) 619
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 326, 382, 566
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 1 cut(s) 120
TspGWI ACGGA 2 cut(s) 262, 478
Tth111I GACNNNGTC 1 cut(s) 182
VpaK11BI GGWCC 2 cut(s) 4, 270
XapI RAATTY 1 cut(s) 554
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.