Rmu_sc0007029.1_g000014

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007029.1
OG0005522
Physical Location & Seq
Reverse (-)
73138 .. 74862
1725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007029.1_g000014.1.cds

Sequence Viewer

Length: 1725 bp
atgatcaaaaccgagcatggcgtcccaagaaagcaccaaaccctagtttacagaggcagagtgcttgaaaaccacgaaaccctagcagcatgcaaggtcgaaaggtggtccattatccatgctattttctgtccagaagaaattagcaatcccaatgtgggtggggtagacatggctgtgaaagtagtgtctgatgccttgagggtggaagtgaaaccctggtataccggccaagaaataaaggcgatcatggagagcatggcggggatttccttgggcgatcatgtcctcagccattgggggaagctcatagaggattcgagtcgcgtattaagcactgaaggcagcagcaacatgatcaataatgaggacgagcccccgatttatttactgtcaaagtttcagattaccgcacgaggctgtggttgggaagaggtgattatggtgagtgaatcacatactgtcctagatgtaaagaagatggttgaagattttgccgaggtgaaacctgaaaacctgatgctttgtcatggcaatggtggcgatgataagatggtttgtctcgatgatgatgagaaaacgcttctgttttatgatgtaaaagagaaagctacgatttgggtacttggcagatcttggaaattgaccatcaagatgccacaggtgggagataaattcgtgcttagctacgataaggtcggtaccataaaggacattaaggattatatatgtaagaagatggggattccatgtagcagtcagaagctttttcgtgaagggcagttgcttgatgatgaagcagcatccctagaaacattcgacaaccagctggaagctgagaatagtgttatttatgtgactttcggggctggcgatgatgaccaagtcgacgaaatagaagatataagcttaatatttgagagagacaaaaatgaaaagggtttgattgaatacaaggtggtttataagagcgaatcagatgagaacagtgaatcaggtgaaagcagcgattctgatagtgattcatcagatttagtctcttctttcgaaatcaagttgaaatcatcttgcacgattttcgatgtcaaggcagcaatcgagagcctaaacagctttcgaatttgtacccaaaagctatactgggatgatcaaattatggaagattgggaaacagtggacgacgacattacaaagtggtcctgtgtccctgctccattaacaatacactttgagacacccagttctagttctaatgtgggggccaatgaaaagattgtttctgcagttgaccgtgcagacgaaacccttgacgacgttttggttccacaaaaggtgctggaaagaatccatgggcagagaggtttcttcctcgatggtattttactcgaggcatgcacattgctttctcagattgaagctggttctgtcttatgcttaaacagtgtggtgcagataaatgttactgggccgaatggtgcaacgaccaagctgcatgtgaacccttcttacaaattcaaggatgtaaaagagaagattgaactagattgtggaatcccatttggtttacagagacttactcatgatggtgatgaacttaatgatgatatgactatagggctttatgaggcatcgttgggcagtctgtggaaattaaggtggaacttttgtggtacttcctggtcattcttctacccgtggtacaaggattgttgcaggactggctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

574

Amino Acids

64.48

Weight (kDa)

4.66

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 966
Acc65I GGTACC 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 701
AccI GTMKAC 3 cut(s) 168, 224, 888
AccII CGCG 1 cut(s) 327
AciI CCGC 2 cut(s) 263, 411
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 3 cut(s) 674, 1119, 1511
AcuI CTGAAG 1 cut(s) 360
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 5 cut(s) 624, 703, 1126, 1672, 1700
AfiI CCNNNNNNNGG 2 cut(s) 158, 665
AgsI TTSAA 7 cut(s) 68, 488, 950, 1060, 1415, 1516, 1538
AjnI CCWGG 2 cut(s) 218, 1676
Alw26I GTCTC 5 cut(s) 566, 918, 1042, 1226, 1564
Ama87I CYCGRG 1 cut(s) 1385
AoxI GGCC 3 cut(s) 229, 1260, 1466
ApeKI GCWGC 7 cut(s) 86, 345, 348, 800, 1005, 1091, 1489
ApoI RAATTY 3 cut(s) 674, 1119, 1511
Asp718I GGTACC 1 cut(s) 701
AspS9I GGNCC 4 cut(s) 108, 1197, 1260, 1466
AsuHPI GGTGA 5 cut(s) 448, 457, 514, 1010, 1598
AsuII TTCGAA 2 cut(s) 1047, 1117
AvaI CYCGRG 1 cut(s) 1385
AvaII GGWCC 2 cut(s) 108, 1197
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 378
BauI CACGAG 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 290
BbvI GCAGC 7 cut(s) 98, 357, 360, 812, 1017, 1103, 1476
BccI CCATC 6 cut(s) 475, 547, 656, 733, 1367, 1577
BcgI CGANNNNNNTGC 4 cut(s) 1060, 1094, 1471, 1505
BciT130I CCWGG 2 cut(s) 220, 1678
BclI TGATCA 3 cut(s) 3, 357, 1147
BcoDI GTCTC 5 cut(s) 566, 918, 1042, 1226, 1564
BfaI CTAG 7 cut(s) 44, 83, 467, 809, 1245, 1541, 1723
BfmI CTRYAG 2 cut(s) 1281, 1611
BglII AGATCT 1 cut(s) 632
BisI GCNGC 7 cut(s) 87, 346, 349, 801, 1006, 1092, 1490
BlpI GCTNAGC 1 cut(s) 683
BlsI GCNGC 7 cut(s) 88, 347, 350, 802, 1007, 1093, 1491
Bme1390I CCNGG 2 cut(s) 220, 1678
Bme18I GGWCC 2 cut(s) 108, 1197
BmeT110I CYCGRG 1 cut(s) 1385
BmgT120I GGNCC 4 cut(s) 108, 1197, 1260, 1466
BmiI GGNNCC 3 cut(s) 703, 1261, 1323
BmrFI CCNGG 2 cut(s) 220, 1678
BmrI ACTGGG 3 cut(s) 1150, 1233, 1473
BmsI GCATC 5 cut(s) 184, 510, 645, 812, 1637
BmuI ACTGGG 3 cut(s) 1150, 1233, 1473
BplI GAGNNNNNCTC 2 cut(s) 1561, 1593
Bpu10I CCTNAGC 1 cut(s) 290
Bpu1102I GCTNAGC 1 cut(s) 683
Bpu14I TTCGAA 2 cut(s) 1047, 1117
BpuEI CTTGAG 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 5 cut(s) 218, 273, 498, 1348, 1694
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 665
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 4 cut(s) 1145, 1239, 1468, 1723
Bse3DI GCAATG 2 cut(s) 541, 1397
BseBI CCWGG 2 cut(s) 220, 1678
BseDI CCNNGG 5 cut(s) 218, 273, 498, 1348, 1694
BseGI GGATG 3 cut(s) 803, 1150, 1525
BseLI CCNNNNNNNGG 2 cut(s) 158, 665
BseMI GCAATG 2 cut(s) 541, 1397
BseMII CTCAG 3 cut(s) 304, 828, 1421
BseNI ACTGG 4 cut(s) 1145, 1239, 1468, 1723
BseXI GCAGC 7 cut(s) 98, 357, 360, 812, 1017, 1103, 1476
BsgI GTGCAG 2 cut(s) 1314, 1469
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 3 cut(s) 231, 1262, 1468
BshNI GGYRCC 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 1385
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 2 cut(s) 8, 1190
BslI CCNNNNNNNGG 2 cut(s) 158, 665
BsmAI GTCTC 5 cut(s) 566, 918, 1042, 1226, 1564
BsmFI GGGAC 2 cut(s) 8, 1190
BsnI GGCC 3 cut(s) 231, 1262, 1468
BsoBI CYCGRG 1 cut(s) 1385
Bsp119I TTCGAA 2 cut(s) 1047, 1117
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
Bsp1720I GCTNAGC 1 cut(s) 683
Bsp19I CCATGG 1 cut(s) 1348
BspACI CCGC 2 cut(s) 263, 411
BspANI GGCC 3 cut(s) 231, 1262, 1468
BspCNI CTCAG 3 cut(s) 303, 829, 1420
BspFNI CGCG 1 cut(s) 327
BspHI TCATGA 1 cut(s) 1579
BspLI GGNNCC 3 cut(s) 703, 1261, 1323
BspMAI CTGCAG 1 cut(s) 1285
BspT104I TTCGAA 2 cut(s) 1047, 1117
BspT107I GGYRCC 1 cut(s) 701
BsrDI GCAATG 2 cut(s) 541, 1397
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 4 cut(s) 1145, 1239, 1468, 1723
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 5 cut(s) 218, 273, 498, 1348, 1694
BssMI GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
BssNAI GTATAC 1 cut(s) 225
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 2 cut(s) 273, 1348
Bst1107I GTATAC 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 414
Bst2UI CCWGG 2 cut(s) 220, 1678
Bst4CI ACNGT 6 cut(s) 393, 463, 989, 1174, 1292, 1442
Bst6I CTCTTC 2 cut(s) 426, 1045
BstACI GRCGYC 1 cut(s) 21
BstBI TTCGAA 2 cut(s) 1047, 1117
BstC8I GCNNGC 3 cut(s) 91, 871, 1393
BstDEI CTNAG 4 cut(s) 290, 683, 837, 1407
BstDSI CCRYGG 2 cut(s) 1348, 1694
BstF5I GGATG 3 cut(s) 803, 1150, 1525
BstFNI CGCG 1 cut(s) 327
BstKTI GATC 6 cut(s) 6, 249, 283, 360, 635, 1150
BstMAI GTCTC 5 cut(s) 566, 918, 1042, 1226, 1564
BstMBI GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 333, 342, 540, 1110, 1719
BstNI CCWGG 2 cut(s) 220, 1678
BstNSI RCATGY 3 cut(s) 93, 1395, 1496
BstSCI CCNGG 2 cut(s) 218, 1676
BstSFI CTRYAG 2 cut(s) 1281, 1611
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 7 cut(s) 98, 357, 360, 812, 1017, 1103, 1476
BstX2I RGATCY 1 cut(s) 632
BstYI RGATCY 1 cut(s) 632
BstZ17I GTATAC 1 cut(s) 225
BsuRI GGCC 3 cut(s) 231, 1262, 1468
BtgI CCRYGG 2 cut(s) 1348, 1694
BtgZI GCGATG 2 cut(s) 558, 888
BtsCI GGATG 3 cut(s) 803, 1150, 1525
BtsIMutI CAGTG 4 cut(s) 336, 994, 1179, 1447
Cac8I GCNNGC 3 cut(s) 91, 871, 1393
CciI TCATGA 1 cut(s) 1579
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 4 cut(s) 108, 1197, 1260, 1466
CseI GACGC 1 cut(s) 10
Csp6I GTAC 5 cut(s) 623, 702, 1125, 1671, 1699
CspCI CAANNNNNGTGG 2 cut(s) 142, 177
CviQI GTAC 5 cut(s) 623, 702, 1125, 1671, 1699
DdeI CTNAG 4 cut(s) 290, 683, 837, 1407
DpnI GATC 6 cut(s) 5, 248, 282, 359, 634, 1149
DpnII GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 426, 1045
EarI CTCTTC 2 cut(s) 426, 1045
Eco130I CCWWGG 2 cut(s) 273, 1348
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 2 cut(s) 108, 1197
Eco57I CTGAAG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 1385
EcoRII CCWGG 2 cut(s) 218, 1676
EcoT14I CCWWGG 2 cut(s) 273, 1348
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 273, 1348
FaqI GGGAC 2 cut(s) 8, 1190
FauI CCCGC 1 cut(s) 256
FbaI TGATCA 3 cut(s) 3, 357, 1147
FblI GTMKAC 3 cut(s) 168, 224, 888
Fnu4HI GCNGC 7 cut(s) 87, 346, 349, 801, 1006, 1092, 1490
FokI GGATG 3 cut(s) 790, 1157, 1532
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 7 cut(s) 87, 346, 349, 801, 1006, 1092, 1490
FspBI CTAG 7 cut(s) 44, 83, 467, 809, 1245, 1541, 1723
GluI GCNGC 7 cut(s) 87, 346, 349, 801, 1006, 1092, 1490
HaeIII GGCC 3 cut(s) 231, 1262, 1468
HapII CCGG 1 cut(s) 228
HgaI GACGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 21
HincII GTYRAC 2 cut(s) 889, 1288
HindII GTYRAC 2 cut(s) 889, 1288
HindIII AAGCTT 2 cut(s) 764, 907
HpaII CCGG 1 cut(s) 228
HphI GGTGA 5 cut(s) 448, 457, 514, 1010, 1598
Hpy166II GTNNAC 8 cut(s) 49, 169, 225, 889, 1177, 1288, 1498, 1565
Hpy188I TCNGA 7 cut(s) 193, 405, 762, 979, 1015, 1030, 1410
Hpy188III TCNNGA 6 cut(s) 134, 563, 652, 773, 1099, 1580
Hpy8I GTNNAC 8 cut(s) 49, 169, 225, 889, 1177, 1288, 1498, 1565
Hpy99I CGWCG 3 cut(s) 893, 1184, 1316
HpyAV CCTTC 3 cut(s) 335, 770, 1512
HpyCH4III ACNGT 6 cut(s) 393, 463, 989, 1174, 1292, 1442
HpyCH4IV ACGT 1 cut(s) 1314
HpyCH4V TGCA 9 cut(s) 93, 1071, 1283, 1295, 1395, 1450, 1478, 1492, 1713
HpyF10VI GCNNNNNNNGC 5 cut(s) 333, 342, 540, 1110, 1719
HpyF3I CTNAG 4 cut(s) 290, 683, 837, 1407
HpySE526I ACGT 1 cut(s) 1314
Hsp92I GRCGYC 1 cut(s) 21
KpnI GGTACC 1 cut(s) 705
Ksp22I TGATCA 3 cut(s) 3, 357, 1147
Kzo9I GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
LmnI GCTCC 1 cut(s) 1216
Lsp1109I GCAGC 7 cut(s) 98, 357, 360, 812, 1017, 1103, 1476
LweI GCATC 5 cut(s) 184, 510, 645, 812, 1637
MaeI CTAG 7 cut(s) 44, 83, 467, 809, 1245, 1541, 1723
MaeII ACGT 1 cut(s) 1314
MaeIII GTNAC 2 cut(s) 856, 1459
MalI GATC 6 cut(s) 5, 248, 282, 359, 634, 1149
MboI GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
MflI RGATCY 1 cut(s) 632
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 7 cut(s) 141, 641, 674, 1119, 1152, 1511, 1649
MlyI GAGTC 1 cut(s) 331
MseI TTAA 7 cut(s) 332, 717, 911, 1217, 1436, 1596, 1652
MslI CAYNNNNRTG 4 cut(s) 176, 534, 653, 1584
MspA1I CMGCKG 1 cut(s) 829
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 220, 1678
MvaI CCWGG 2 cut(s) 220, 1678
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 5 cut(s) 333, 342, 540, 1110, 1719
NcoI CCATGG 1 cut(s) 1348
NdeII GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
NlaIV GGNNCC 3 cut(s) 703, 1261, 1323
NmeAIII GCCGAG 1 cut(s) 523
NmuCI GTSAC 1 cut(s) 856
NspI RCATGY 3 cut(s) 93, 1395, 1496
NspV TTCGAA 2 cut(s) 1047, 1117
PaeI GCATGC 2 cut(s) 93, 1395
PaeR7I CTCGAG 1 cut(s) 1385
PagI TCATGA 1 cut(s) 1579
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PfeI GAWTC 9 cut(s) 317, 452, 745, 974, 992, 1010, 1022, 1344, 1551
PflFI GACNNNGTC 1 cut(s) 884
PkrI GCNGC 7 cut(s) 88, 347, 350, 802, 1007, 1093, 1491
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
PsiI TTATAA 1 cut(s) 966
Psp6I CCWGG 2 cut(s) 218, 1676
PspGI CCWGG 2 cut(s) 218, 1676
PspN4I GGNNCC 3 cut(s) 703, 1261, 1323
PspPI GGNCC 4 cut(s) 108, 1197, 1260, 1466
PspXI VCTCGAGB 1 cut(s) 1385
PsrI GAACNNNNNNTAC 2 cut(s) 1490, 1522
PstI CTGCAG 1 cut(s) 1285
PsuI RGATCY 1 cut(s) 632
PsyI GACNNNGTC 1 cut(s) 884
PvuII CAGCTG 1 cut(s) 829
RsaI GTAC 5 cut(s) 624, 703, 1126, 1672, 1700
RsaNI GTAC 5 cut(s) 623, 702, 1125, 1671, 1699
RseI CAYNNNNRTG 4 cut(s) 176, 534, 653, 1584
SalI GTCGAC 1 cut(s) 887
SaqAI TTAA 7 cut(s) 332, 717, 911, 1217, 1436, 1596, 1652
SatI GCNGC 7 cut(s) 87, 346, 349, 801, 1006, 1092, 1490
Sau3AI GATC 6 cut(s) 3, 246, 280, 357, 632, 1147
Sau96I GGNCC 4 cut(s) 108, 1197, 1260, 1466
SchI GAGTC 1 cut(s) 331
ScrFI CCNGG 2 cut(s) 220, 1678
SduI GDGCHC 1 cut(s) 378
SfaNI GCATC 5 cut(s) 184, 510, 645, 812, 1637
SfcI CTRYAG 2 cut(s) 1281, 1611
Sfr274I CTCGAG 1 cut(s) 1385
SfuI TTCGAA 2 cut(s) 1047, 1117
SinI GGWCC 2 cut(s) 108, 1197
SlaI CTCGAG 1 cut(s) 1385
SmiMI CAYNNNNRTG 4 cut(s) 176, 534, 653, 1584
SmlI CTYRAG 2 cut(s) 199, 1385
SmoI CTYRAG 2 cut(s) 199, 1385
SphI GCATGC 2 cut(s) 93, 1395
Sse9I AATT 7 cut(s) 141, 641, 674, 1119, 1152, 1511, 1649
SsiI CCGC 2 cut(s) 263, 411
SspI AATATT 1 cut(s) 915
SspMI CTAG 7 cut(s) 44, 83, 467, 809, 1245, 1541, 1723
StyD4I CCNGG 2 cut(s) 218, 1676
StyI CCWWGG 2 cut(s) 273, 1348
TaaI ACNGT 6 cut(s) 393, 463, 989, 1174, 1292, 1442
TaiI ACGT 1 cut(s) 1317
TasI AATT 7 cut(s) 141, 641, 674, 1119, 1152, 1511, 1649
TfiI GAWTC 9 cut(s) 317, 452, 745, 974, 992, 1010, 1022, 1344, 1551
Tru1I TTAA 7 cut(s) 332, 717, 911, 1217, 1436, 1596, 1652
Tru9I TTAA 7 cut(s) 332, 717, 911, 1217, 1436, 1596, 1652
TscAI CASTG 4 cut(s) 343, 994, 1179, 1447
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 7 cut(s) 86, 345, 348, 800, 1005, 1091, 1489
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 5 cut(s) 810, 948, 1014, 1281, 1605
TspRI CASTG 4 cut(s) 343, 994, 1179, 1447
Tth111I GACNNNGTC 1 cut(s) 884
VpaK11BI GGWCC 2 cut(s) 108, 1197
XapI RAATTY 3 cut(s) 674, 1119, 1511
XceI RCATGY 3 cut(s) 93, 1395, 1496
XhoI CTCGAG 1 cut(s) 1385
XmiI GTMKAC 3 cut(s) 168, 224, 888
XspI CTAG 7 cut(s) 44, 83, 467, 809, 1245, 1541, 1723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.