Rh6BG305100

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005522
Physical Location & Seq
Forward (+)
53502257 .. 53504245
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG305100.1

Sequence Viewer

Length: 1989 bp
ATGAAGAAGCCAGAAGTTCGCGACTCTGATGATGAGGAATATGACTTCTCAGAACCTTGCCACTCTAGTGAAGAAGAATATAACTCCTCAGAATCTTGTGACGACTCTTCTGATGATGATGAATCAGACTCCTCAGAAGAAAACTCGATCGAAACTGAAACGGCTGAGTCCACTGTGACGATCTTGGTGAAGTTTTCGTCAAGCAAAGAGGAGAGGAACATGTTCAAACTAAAGAGTTCAGATACCATCTCCCGCCTCAAGACCATGATCAAAACCGAGCATGGCGTCCCAAGAAAGCACCAAACCCTAGTTTACAGAGGCAGAGTGCTTGAAAACCACGAAACCCTAGCAGCATGCAAGGTCGAAAGGTGGTCCATTATCCATGCTATTTTCTGTCCAGAAGAAATTAGCAATCCCAATGTGGGTGGGGTAGACATGGCTGTGAAAGTAGTGTCTGATGCCTTGAGGGTGGAAGTGAAACCCTGGTATACCGGCCAAGAAATAAAGGCGATCATGGAGAGCATGGCGGGGATTTCCTTGGGCGATCATGTCCTCAGCCATTGGGGGAAGCTCATAGAGGATTCGAGTCACGTATTAAGCACTGAAGGCAGCAGTAACATGATCAATAATGAGGACCAGCCCCCGATTTATTTACTGTCAAAGTTTCAGATTACTGCACGAGGCTGTGGTTGGGAAGAGGTGATTATGGTGAGTGAATCACATACTGTCCTAGATGTAAAGAAGATGGTTGAAGATTTTGCCGAGGTGAAACCTGAAAACCTGATGCTTTGTCATGGCAATGGTGGCGATGATAAGACGGTTTGTCTCGATGATGATGAGAAAACTCTTCTGTTTTATGATGTAAAAGGGAAAGCTACGATTTGGGTACTTGGCAGATCTTGGAAATTGACCATCAAGATGCCACAGGTGGGAGATAAATTCGTGCTTAGCTACGATAAGGTCGGTACCATAAAGGACATTAAGGATTATATATGTAAGAAGATGGGGATTCCATGTAGCAGTCAGAAGCTTTTTCGTGAAGGGCAGTTGCTTGATGATGAAGCAGCATCCCTAGAAGCATTCAACAACCAGCTGGAAGCTGAGAATAGTGTTATTTATGTGACTTTCGGGGCTGGCGATGATGACCAAGTCGACGAAATAGAAGATATAAGCTTAATATTTGAGAGAGACAAAAATGAAAAGGGTTTGATTGAATACAAGGTGGTTTATAAGAGCGAATCAGATGAGAACAGTGAATCAGGTGAAAGCAGCGATTCTGATAGTGATTCATCAGATTTAGTCTCTTCTTTCGAAATCAAGTTGAAATCATCTTGCACGATTTTCGATGTCAAGGCAGCAATCGAGAGCCTAAACAGCTTTCGAATTTGTACCCAAAAGCTATACTGGGATGATCAAATTCTGGAAGATTGGGAAACAGTGGACGACGACATTACAAAGTGGTCCAGTGTCCCTGCTCCATTAACAATACACTTTGAGACACCCAGTTCTAGTTCTAATGTGGGGGCCAATGAAAAGATTGTTTCTGCAGTTGACCGTGCAGACGAAACCCTTGACGACGTTTTGGTTCCACAAAAGGTGCTGGAAAGAATCCATGGGCAGCCAGGTTTCTTCCTCGATGGTATTTTAGTCGAGGGATGCACATTGCTTTCTCAGATTGAAGCTGGTTCTGTCTTATGCTTAAACAGTGTGGTGCAGATAAATGTTACTGGGCCGAATGGTGCAACGACCAAGGTGCATGTGAACCCTTCTTACAAATTCAAGGATGTAAAAGAGAAGATTGAACTAGATTGTGGAATCCCATTTGGTTTACAGAGACTTACTCATGATGGTGATGAACTTAATGATGATATGACTATAGGGTTTTATGAGGCATCGTTGGGCAGTCTGTGGAAATTAAGGTGGAACTTTTGTGGTACATCCTGGTCATTCTTCTACCCGTGGTACAAGGATTGTTGCAGGACTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

662

Amino Acids

74.23

Weight (kDa)

4.55

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 62 - 129 9.3e-13 Ubiquitin family
Rad60-SLD PF11976 572 - 629 2.6e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 573 - 629 7.2e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 1 cut(s) 965
AccI GTMKAC 3 cut(s) 432, 488, 1152
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 253, 527
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 4 cut(s) 938, 1383, 1416, 1775
AcuI CTGAAG 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 5 cut(s) 888, 967, 1390, 1936, 1964
AfiI CCNNNNNNNGG 2 cut(s) 422, 929
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 9 cut(s) 226, 332, 752, 1084, 1214, 1324, 1679, 1780, 1802
AhdI GACNNNNNGTC 1 cut(s) 822
AjnI CCWGG 3 cut(s) 482, 1621, 1940
AleI CACNNNNGTG 1 cut(s) 66
Alw26I GTCTC 5 cut(s) 830, 1182, 1306, 1490, 1828
AoxI GGCC 3 cut(s) 493, 1524, 1730
ApeKI GCWGC 6 cut(s) 350, 609, 1064, 1269, 1355, 1618
ApoI RAATTY 4 cut(s) 938, 1383, 1416, 1775
Asp700I GAANNNNTTC 1 cut(s) 221
Asp718I GGTACC 1 cut(s) 965
AspS9I GGNCC 5 cut(s) 372, 634, 1461, 1524, 1730
AsuHPI GGTGA 6 cut(s) 199, 712, 721, 778, 1274, 1862
AsuII TTCGAA 2 cut(s) 1311, 1381
AvaII GGWCC 3 cut(s) 372, 634, 1461
BanI GGYRCC 1 cut(s) 965
BauI CACGAG 1 cut(s) 678
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 6 cut(s) 362, 621, 1076, 1281, 1367, 1630
BccI CCATC 6 cut(s) 254, 739, 920, 997, 1631, 1841
BceAI ACGGC 1 cut(s) 177
BcgI CGANNNNNNTGC 4 cut(s) 1324, 1358, 1735, 1769
BciT130I CCWGG 3 cut(s) 484, 1623, 1942
BclI TGATCA 3 cut(s) 267, 621, 1411
BcoDI GTCTC 5 cut(s) 830, 1182, 1306, 1490, 1828
BfaI CTAG 8 cut(s) 66, 308, 347, 731, 1073, 1509, 1805, 1987
BfmI CTRYAG 2 cut(s) 1545, 1875
BglII AGATCT 1 cut(s) 896
BisI GCNGC 6 cut(s) 351, 610, 1065, 1270, 1356, 1619
BlpI GCTNAGC 1 cut(s) 947
BlsI GCNGC 6 cut(s) 352, 611, 1066, 1271, 1357, 1620
Bme1390I CCNGG 3 cut(s) 484, 1623, 1942
Bme18I GGWCC 3 cut(s) 372, 634, 1461
BmeRI GACNNNNNGTC 1 cut(s) 822
BmgT120I GGNCC 5 cut(s) 372, 634, 1461, 1524, 1730
BmiI GGNNCC 3 cut(s) 967, 1525, 1587
BmrFI CCNGG 3 cut(s) 484, 1623, 1942
BmrI ACTGGG 3 cut(s) 1414, 1497, 1737
BmsI GCATC 6 cut(s) 448, 774, 909, 1076, 1646, 1901
BmuI ACTGGG 3 cut(s) 1414, 1497, 1737
BplI GAGNNNNNCTC 2 cut(s) 1825, 1857
Bpu10I CCTNAGC 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 947
Bpu14I TTCGAA 2 cut(s) 1311, 1381
BpuEI CTTGAG 2 cut(s) 242, 484
BsaAI YACGTR 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 285
BsaJI CCNNGG 6 cut(s) 482, 537, 762, 1612, 1749, 1958
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 929
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 5 cut(s) 1409, 1464, 1503, 1732, 1987
Bse3DI GCAATG 2 cut(s) 805, 1661
BseBI CCWGG 3 cut(s) 484, 1623, 1942
BseDI CCNNGG 6 cut(s) 482, 537, 762, 1612, 1749, 1958
BseGI GGATG 5 cut(s) 1067, 1414, 1661, 1789, 1937
BseLI CCNNNNNNNGG 2 cut(s) 422, 929
BseMI GCAATG 2 cut(s) 805, 1661
BseMII CTCAG 7 cut(s) 63, 102, 147, 156, 568, 1092, 1685
BseNI ACTGG 5 cut(s) 1409, 1464, 1503, 1732, 1987
BseRI GAGGAG 3 cut(s) 76, 121, 224
BseXI GCAGC 6 cut(s) 362, 621, 1076, 1281, 1367, 1630
BsgI GTGCAG 3 cut(s) 660, 1578, 1733
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 495, 1526, 1732
BshNI GGYRCC 1 cut(s) 965
BsiEI CGRYCG 1 cut(s) 150
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 272, 1454
BslI CCNNNNNNNGG 2 cut(s) 422, 929
BsmAI GTCTC 5 cut(s) 830, 1182, 1306, 1490, 1828
BsmFI GGGAC 2 cut(s) 272, 1454
BsmI GAATGC 1 cut(s) 1079
BsnI GGCC 3 cut(s) 495, 1526, 1732
Bsp119I TTCGAA 2 cut(s) 1311, 1381
Bsp143I GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
Bsp1720I GCTNAGC 1 cut(s) 947
Bsp19I CCATGG 1 cut(s) 1612
Bsp68I TCGCGA 1 cut(s) 21
BspACI CCGC 2 cut(s) 253, 527
BspANI GGCC 3 cut(s) 495, 1526, 1732
BspCNI CTCAG 7 cut(s) 62, 101, 146, 157, 567, 1093, 1684
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 1843
BspLI GGNNCC 3 cut(s) 967, 1525, 1587
BspMAI CTGCAG 1 cut(s) 1549
BspT104I TTCGAA 2 cut(s) 1311, 1381
BspT107I GGYRCC 1 cut(s) 965
BsrDI GCAATG 2 cut(s) 805, 1661
BsrFI RCCGGY 1 cut(s) 491
BsrI ACTGG 5 cut(s) 1409, 1464, 1503, 1732, 1987
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 6 cut(s) 482, 537, 762, 1612, 1749, 1958
BssMI GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
BssNAI GTATAC 1 cut(s) 489
BssNI GRCGYC 1 cut(s) 285
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 3 cut(s) 537, 1612, 1749
Bst1107I GTATAC 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 3 cut(s) 484, 1623, 1942
Bst4CI ACNGT 8 cut(s) 175, 657, 727, 820, 1253, 1438, 1556, 1706
Bst6I CTCTTC 4 cut(s) 112, 690, 852, 1309
BstACI GRCGYC 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 592
BstBI TTCGAA 2 cut(s) 1311, 1381
BstC8I GCNNGC 2 cut(s) 355, 1135
BstDEI CTNAG 8 cut(s) 49, 88, 133, 165, 554, 947, 1101, 1671
BstDSI CCRYGG 2 cut(s) 1612, 1958
BstF5I GGATG 5 cut(s) 1067, 1414, 1661, 1789, 1937
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 8 cut(s) 150, 183, 270, 513, 547, 624, 899, 1414
BstMAI GTCTC 5 cut(s) 830, 1182, 1306, 1490, 1828
BstMBI GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
BstMCI CGRYCG 1 cut(s) 150
BstMWI GCNNNNNNNGC 4 cut(s) 606, 804, 1374, 1983
BstNI CCWGG 3 cut(s) 484, 1623, 1942
BstNSI RCATGY 3 cut(s) 223, 357, 1760
BstSCI CCNGG 3 cut(s) 482, 1621, 1940
BstSFI CTRYAG 2 cut(s) 1545, 1875
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 6 cut(s) 362, 621, 1076, 1281, 1367, 1630
BstX2I RGATCY 1 cut(s) 896
BstYI RGATCY 1 cut(s) 896
BstZ17I GTATAC 1 cut(s) 489
BsuRI GGCC 3 cut(s) 495, 1526, 1732
BtgI CCRYGG 2 cut(s) 1612, 1958
BtgZI GCGATG 2 cut(s) 822, 1152
BtsCI GGATG 5 cut(s) 1067, 1414, 1661, 1789, 1937
BtsIMutI CAGTG 6 cut(s) 171, 600, 1258, 1443, 1471, 1711
BtuMI TCGCGA 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 355, 1135
CciI TCATGA 1 cut(s) 1843
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 5 cut(s) 372, 634, 1461, 1524, 1730
CseI GACGC 1 cut(s) 274
Csp6I GTAC 5 cut(s) 887, 966, 1389, 1935, 1963
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviQI GTAC 5 cut(s) 887, 966, 1389, 1935, 1963
DdeI CTNAG 8 cut(s) 49, 88, 133, 165, 554, 947, 1101, 1671
DpnI GATC 8 cut(s) 149, 182, 269, 512, 546, 623, 898, 1413
DpnII GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
DriI GACNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 4 cut(s) 112, 690, 852, 1309
Eam1105I GACNNNNNGTC 1 cut(s) 822
EarI CTCTTC 4 cut(s) 112, 690, 852, 1309
Eco130I CCWWGG 3 cut(s) 537, 1612, 1749
Eco47I GGWCC 3 cut(s) 372, 634, 1461
Eco57I CTGAAG 1 cut(s) 624
EcoRII CCWGG 3 cut(s) 482, 1621, 1940
EcoT14I CCWWGG 3 cut(s) 537, 1612, 1749
ErhI CCWWGG 3 cut(s) 537, 1612, 1749
FaqI GGGAC 2 cut(s) 272, 1454
FauI CCCGC 2 cut(s) 260, 520
FbaI TGATCA 3 cut(s) 267, 621, 1411
FblI GTMKAC 3 cut(s) 432, 488, 1152
Fnu4HI GCNGC 6 cut(s) 351, 610, 1065, 1270, 1356, 1619
FokI GGATG 5 cut(s) 1054, 1421, 1668, 1796, 1924
Fsp4HI GCNGC 6 cut(s) 351, 610, 1065, 1270, 1356, 1619
FspBI CTAG 8 cut(s) 66, 308, 347, 731, 1073, 1509, 1805, 1987
GluI GCNGC 6 cut(s) 351, 610, 1065, 1270, 1356, 1619
HaeIII GGCC 3 cut(s) 495, 1526, 1732
HapII CCGG 1 cut(s) 492
HgaI GACGC 1 cut(s) 274
Hin1I GRCGYC 1 cut(s) 285
HincII GTYRAC 2 cut(s) 1153, 1552
HindII GTYRAC 2 cut(s) 1153, 1552
HindIII AAGCTT 2 cut(s) 1028, 1171
HpaII CCGG 1 cut(s) 492
HphI GGTGA 6 cut(s) 199, 712, 721, 778, 1274, 1862
Hpy166II GTNNAC 9 cut(s) 171, 313, 433, 489, 1153, 1441, 1552, 1762, 1829
Hpy188III TCNNGA 9 cut(s) 20, 259, 398, 827, 916, 1037, 1363, 1421, 1844
Hpy8I GTNNAC 9 cut(s) 171, 313, 433, 489, 1153, 1441, 1552, 1762, 1829
Hpy99I CGWCG 3 cut(s) 1157, 1448, 1580
HpyAV CCTTC 3 cut(s) 599, 1034, 1776
HpyCH4III ACNGT 8 cut(s) 175, 657, 727, 820, 1253, 1438, 1556, 1706
HpyCH4IV ACGT 2 cut(s) 591, 1578
HpyF10VI GCNNNNNNNGC 4 cut(s) 606, 804, 1374, 1983
HpyF3I CTNAG 8 cut(s) 49, 88, 133, 165, 554, 947, 1101, 1671
HpySE526I ACGT 2 cut(s) 591, 1578
Hsp92I GRCGYC 1 cut(s) 285
KpnI GGTACC 1 cut(s) 969
Ksp22I TGATCA 3 cut(s) 267, 621, 1411
Kzo9I GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
LmnI GCTCC 1 cut(s) 1480
Lsp1109I GCAGC 6 cut(s) 362, 621, 1076, 1281, 1367, 1630
LweI GCATC 6 cut(s) 448, 774, 909, 1076, 1646, 1901
MaeI CTAG 8 cut(s) 66, 308, 347, 731, 1073, 1509, 1805, 1987
MaeII ACGT 2 cut(s) 591, 1578
MaeIII GTNAC 6 cut(s) 98, 175, 587, 614, 1120, 1723
MalI GATC 8 cut(s) 149, 182, 269, 512, 546, 623, 898, 1413
MboI GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
MflI RGATCY 1 cut(s) 896
MluCI AATT 7 cut(s) 405, 905, 938, 1383, 1416, 1775, 1913
MlyI GAGTC 5 cut(s) 17, 98, 122, 176, 595
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 7 cut(s) 596, 981, 1175, 1481, 1700, 1860, 1916
MslI CAYNNNNRTG 5 cut(s) 66, 440, 798, 917, 1848
MspA1I CMGCKG 1 cut(s) 1093
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 3 cut(s) 484, 1623, 1942
Mva1269I GAATGC 1 cut(s) 1079
MvaI CCWGG 3 cut(s) 484, 1623, 1942
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 606, 804, 1374, 1983
NcoI CCATGG 1 cut(s) 1612
NdeII GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
NlaIV GGNNCC 3 cut(s) 967, 1525, 1587
NmeAIII GCCGAG 1 cut(s) 787
NmuCI GTSAC 4 cut(s) 98, 175, 587, 1120
NruI TCGCGA 1 cut(s) 21
NspI RCATGY 3 cut(s) 223, 357, 1760
NspV TTCGAA 2 cut(s) 1311, 1381
OliI CACNNNNGTG 1 cut(s) 66
PaeI GCATGC 1 cut(s) 357
PagI TCATGA 1 cut(s) 1843
PciI ACATGT 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 1134
PctI GAATGC 1 cut(s) 1079
PdmI GAANNNNTTC 1 cut(s) 221
PflFI GACNNNGTC 1 cut(s) 1148
PkrI GCNGC 6 cut(s) 352, 611, 1066, 1271, 1357, 1620
Ple19I CGATCG 1 cut(s) 150
PleI GAGTC 5 cut(s) 17, 98, 122, 175, 594
PpsI GAGTC 5 cut(s) 17, 98, 122, 175, 594
Ppu21I YACGTR 1 cut(s) 592
PscI ACATGT 1 cut(s) 219
PsiI TTATAA 1 cut(s) 1230
Psp6I CCWGG 3 cut(s) 482, 1621, 1940
PspGI CCWGG 3 cut(s) 482, 1621, 1940
PspN4I GGNNCC 3 cut(s) 967, 1525, 1587
PspPI GGNCC 5 cut(s) 372, 634, 1461, 1524, 1730
PsrI GAACNNNNNNTAC 2 cut(s) 1754, 1786
PstI CTGCAG 1 cut(s) 1549
PsuI RGATCY 1 cut(s) 896
PsyI GACNNNGTC 1 cut(s) 1148
PvuI CGATCG 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 1093
RruI TCGCGA 1 cut(s) 21
RsaI GTAC 5 cut(s) 888, 967, 1390, 1936, 1964
RsaNI GTAC 5 cut(s) 887, 966, 1389, 1935, 1963
RseI CAYNNNNRTG 5 cut(s) 66, 440, 798, 917, 1848
SalI GTCGAC 1 cut(s) 1151
SaqAI TTAA 7 cut(s) 596, 981, 1175, 1481, 1700, 1860, 1916
SatI GCNGC 6 cut(s) 351, 610, 1065, 1270, 1356, 1619
Sau3AI GATC 8 cut(s) 147, 180, 267, 510, 544, 621, 896, 1411
Sau96I GGNCC 5 cut(s) 372, 634, 1461, 1524, 1730
SchI GAGTC 5 cut(s) 17, 98, 122, 176, 595
ScrFI CCNGG 3 cut(s) 484, 1623, 1942
SfaNI GCATC 6 cut(s) 448, 774, 909, 1076, 1646, 1901
SfcI CTRYAG 2 cut(s) 1545, 1875
SfuI TTCGAA 2 cut(s) 1311, 1381
SinI GGWCC 3 cut(s) 372, 634, 1461
SmiMI CAYNNNNRTG 5 cut(s) 66, 440, 798, 917, 1848
SmlI CTYRAG 2 cut(s) 257, 463
SmoI CTYRAG 2 cut(s) 257, 463
SphI GCATGC 1 cut(s) 357
Sse9I AATT 7 cut(s) 405, 905, 938, 1383, 1416, 1775, 1913
SsiI CCGC 2 cut(s) 253, 527
SspI AATATT 1 cut(s) 1179
SspMI CTAG 8 cut(s) 66, 308, 347, 731, 1073, 1509, 1805, 1987
StyD4I CCNGG 3 cut(s) 482, 1621, 1940
StyI CCWWGG 3 cut(s) 537, 1612, 1749
TaaI ACNGT 8 cut(s) 175, 657, 727, 820, 1253, 1438, 1556, 1706
TaiI ACGT 2 cut(s) 594, 1581
TasI AATT 7 cut(s) 405, 905, 938, 1383, 1416, 1775, 1913
Tru1I TTAA 7 cut(s) 596, 981, 1175, 1481, 1700, 1860, 1916
Tru9I TTAA 7 cut(s) 596, 981, 1175, 1481, 1700, 1860, 1916
TscAI CASTG 6 cut(s) 178, 607, 1258, 1443, 1471, 1711
TseFI GTSAC 4 cut(s) 98, 175, 587, 1120
TseI GCWGC 6 cut(s) 350, 609, 1064, 1269, 1355, 1618
Tsp45I GTSAC 4 cut(s) 98, 175, 587, 1120
TspDTI ATGAA 7 cut(s) 17, 135, 1074, 1212, 1278, 1545, 1869
TspRI CASTG 6 cut(s) 178, 607, 1258, 1443, 1471, 1711
Tth111I GACNNNGTC 1 cut(s) 1148
VpaK11BI GGWCC 3 cut(s) 372, 634, 1461
XapI RAATTY 4 cut(s) 938, 1383, 1416, 1775
XceI RCATGY 3 cut(s) 223, 357, 1760
XmiI GTMKAC 3 cut(s) 432, 488, 1152
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 8 cut(s) 66, 308, 347, 731, 1073, 1509, 1805, 1987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.