Rroxscaffold_7G00181430

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005522
Physical Location & Seq
Reverse (-)
20558250 .. 20560612
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181430.1

Sequence Viewer

Length: 1503 bp
ATGAAGAAGCCAGAAGTTCGCGACTCTGATGATGAGGAATATGACTTCTCAGAACCTTGCCACTCTAGTGAAGAATATAACTCCTCAGAATCTTGTGACGACTCTTCTGATGATGATGAATCAGACTCCTCAGAAGAGAACTCGATCGAAACTGAAACGGCTGCGTCCAAAGTGACGATCTTGGTGAAGTTTTCGTCAAGCAAAGAGGAGAGGAACTTCAATCCCAATGTGGGTGGGGTAGACATGGCTGTGAAAGTAGTGTCTGATGCCTTGAGGATGGAAGTGAAACCCTGGTATACCGGCCAAGAAATAAAGGCGATCATGGAGAGCATGGCAGGGATTTCCTTGGGCGATCATGTCCTCAGCCATTGGGGGAAGCTCATAGAGGATTCGAGTCGCATTACTGCACGAGGCTGTGGTTGGGAAGAGGTGATTATGGTGAGTGAATCACATACTGTCCTAGATGTAAAGAAGATGGTTGAAGATTTTGCTGAGGTGAAACCTGAAAACCTGATGCTTTGTCATGGCAATGGTGGCGATGATAAGATGGTTTGTCTCGATGATGATGAGAAAACTCTTTTGTTTTATGATGTAAAAGAGAAAGCTACGATTTGGGTAATTGGCAGATCTTGGAAATTGACCATCAAGATGCCACAGGTGGGAGATAAATTCGTGCTTAGCTACGATAAGGTCGGTACCATAAAGGACATTAAGGATTATATATGCAAGAAGATGGGGGTTCCATGTAGCAGTCAGAAGCTTTTTCGTAAAGGGCAGTTGCTTGATGATGAAGCAGCATCCCTAGAAACATTCAACAACCAGCTGGAAGCTGAGAATAGTGTTATTTATGTGACTTTCGGGGCTGGCGATGATGACCAAGTCGACGAAATAGAAGATATAAGCTTAATATTTGAGAGAGACAAAAATGAAAAGGGTTTGATTGAATACAAGGTGGTTTATAAGAGCGAATCAGATGAGAACAGTGAATCAGGTGAAAGCAGCGATTCTGATAGTGATAGTGATTCATCAGATTTAGTCTCTTCTTTCGAAATCAAGTTGAGATCATCTTGCACGATTTTCGATGTCAAGGCAGCAATCGAGAGCCTAAACAGCTTTCGAATTTGTACCCAAAAGCTATACTGGGATGATCAAATTCTGGAAGATTGGGAAACAGTGGACGACGACATTACAAAGTGGTCCAGTGTCCCTGCTCCATTAACAATACACTTTGAGACACCCAGTTCTAGTTCTAATGTGGGGGCCAATGAAAATATTGTTTCTGCAGTTGACCGTGCAGACGAAACCCTTGAAGAAGTTTTGGTTCCACAAGAGGTGCTGGAAAGAATCCATGGGCAGCCAGGTTTCTTCCTCGATGGTATTTTACTCGAGGCATGCACATTGCTTCCTCAGATTGAAGCTGGTTGTGCAGCATCACAAGTAGTACTAGAACATCAGGAAAATAAATGGATTCTTTCATTTAGTGATTTTCCTTTTCTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

500

Amino Acids

55.94

Weight (kDa)

4.3

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 213 - 264 9.5e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
Acc65I GGTACC 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 695
AccI GTMKAC 3 cut(s) 240, 296, 882
AccII CGCG 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 3 cut(s) 668, 1119, 1152
AfaI GTAC 3 cut(s) 697, 1126, 1443
AfiI CCNNNNNNNGG 2 cut(s) 230, 659
AgsI TTSAA 6 cut(s) 220, 482, 814, 944, 1310, 1415
AjnI CCWGG 2 cut(s) 290, 1357
AleI CACNNNNGTG 1 cut(s) 66
Alw26I GTCTC 4 cut(s) 560, 912, 1042, 1226
Ama87I CYCGRG 1 cut(s) 1385
AoxI GGCC 2 cut(s) 301, 1260
ApeKI GCWGC 6 cut(s) 161, 794, 999, 1091, 1354, 1427
ApoI RAATTY 3 cut(s) 668, 1119, 1152
Asp718I GGTACC 1 cut(s) 695
AspS9I GGNCC 2 cut(s) 1197, 1260
AsuHPI GGTGA 5 cut(s) 196, 442, 451, 508, 1004
AsuII TTCGAA 2 cut(s) 1047, 1117
AvaI CYCGRG 1 cut(s) 1385
AvaII GGWCC 1 cut(s) 1197
BanI GGYRCC 1 cut(s) 695
BauI CACGAG 1 cut(s) 408
BbvCI CCTCAGC 2 cut(s) 362, 492
BbvI GCAGC 6 cut(s) 148, 806, 1011, 1103, 1366, 1439
BccI CCATC 6 cut(s) 271, 469, 541, 650, 727, 1367
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 1060, 1094
BciT130I CCWGG 2 cut(s) 292, 1359
BclI TGATCA 1 cut(s) 1147
BcoDI GTCTC 4 cut(s) 560, 912, 1042, 1226
BfaI CTAG 6 cut(s) 66, 461, 803, 1245, 1445, 1496
BfmI CTRYAG 1 cut(s) 1281
BglII AGATCT 1 cut(s) 626
BisI GCNGC 6 cut(s) 162, 795, 1000, 1092, 1355, 1428
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 6 cut(s) 163, 796, 1001, 1093, 1356, 1429
BmcAI AGTACT 1 cut(s) 1443
Bme1390I CCNGG 2 cut(s) 292, 1359
Bme18I GGWCC 1 cut(s) 1197
BmeT110I CYCGRG 1 cut(s) 1385
BmgT120I GGNCC 2 cut(s) 1197, 1260
BmiI GGNNCC 4 cut(s) 697, 741, 1261, 1323
BmrFI CCNGG 2 cut(s) 292, 1359
BmrI ACTGGG 2 cut(s) 1150, 1233
BmsI GCATC 5 cut(s) 256, 504, 639, 806, 1439
BmuI ACTGGG 2 cut(s) 1150, 1233
Bpu10I CCTNAGC 2 cut(s) 362, 492
Bpu1102I GCTNAGC 1 cut(s) 677
Bpu14I TTCGAA 2 cut(s) 1047, 1117
BpuEI CTTGAG 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 290, 345, 1348
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 659
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 3 cut(s) 1145, 1200, 1239
Bse3DI GCAATG 2 cut(s) 535, 1397
BseBI CCWGG 2 cut(s) 292, 1359
BseDI CCNNGG 3 cut(s) 290, 345, 1348
BseGI GGATG 3 cut(s) 282, 797, 1150
BseLI CCNNNNNNNGG 2 cut(s) 230, 659
BseMI GCAATG 2 cut(s) 535, 1397
BseMII CTCAG 7 cut(s) 63, 99, 144, 376, 483, 822, 1421
BseNI ACTGG 3 cut(s) 1145, 1200, 1239
BseRI GAGGAG 3 cut(s) 73, 118, 221
BseXI GCAGC 6 cut(s) 148, 806, 1011, 1103, 1366, 1439
BsgI GTGCAG 3 cut(s) 390, 1314, 1446
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 303, 1262
BshNI GGYRCC 1 cut(s) 695
BsiEI CGRYCG 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 1385
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 1190
BslI CCNNNNNNNGG 2 cut(s) 230, 659
BsmAI GTCTC 4 cut(s) 560, 912, 1042, 1226
BsmFI GGGAC 1 cut(s) 1190
BsnI GGCC 2 cut(s) 303, 1262
BsoBI CYCGRG 1 cut(s) 1385
Bsp119I TTCGAA 2 cut(s) 1047, 1117
Bsp143I GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 1348
Bsp68I TCGCGA 1 cut(s) 21
BspANI GGCC 2 cut(s) 303, 1262
BspCNI CTCAG 7 cut(s) 62, 98, 143, 375, 484, 823, 1420
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 4 cut(s) 697, 741, 1261, 1323
BspMAI CTGCAG 1 cut(s) 1285
BspT104I TTCGAA 2 cut(s) 1047, 1117
BspT107I GGYRCC 1 cut(s) 695
BsrDI GCAATG 2 cut(s) 535, 1397
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 3 cut(s) 1145, 1200, 1239
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 290, 345, 1348
BssMI GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
BssNAI GTATAC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 2 cut(s) 345, 1348
Bst1107I GTATAC 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 292, 1359
Bst4CI ACNGT 4 cut(s) 457, 983, 1174, 1292
Bst6I CTCTTC 4 cut(s) 109, 129, 420, 1045
BstBI TTCGAA 2 cut(s) 1047, 1117
BstC8I GCNNGC 2 cut(s) 865, 1393
BstDEI CTNAG 8 cut(s) 49, 85, 130, 362, 492, 677, 831, 1407
BstDSI CCRYGG 1 cut(s) 1348
BstF5I GGATG 3 cut(s) 282, 797, 1150
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 7 cut(s) 147, 180, 321, 355, 629, 1064, 1150
BstMAI GTCTC 4 cut(s) 560, 912, 1042, 1226
BstMBI GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 3 cut(s) 534, 1110, 1424
BstNI CCWGG 2 cut(s) 292, 1359
BstNSI RCATGY 1 cut(s) 1395
BstSCI CCNGG 2 cut(s) 290, 1357
BstSFI CTRYAG 1 cut(s) 1281
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 6 cut(s) 148, 806, 1011, 1103, 1366, 1439
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BstZ17I GTATAC 1 cut(s) 297
BsuRI GGCC 2 cut(s) 303, 1262
BtgI CCRYGG 1 cut(s) 1348
BtgZI GCGATG 2 cut(s) 552, 882
BtsCI GGATG 3 cut(s) 282, 797, 1150
BtsIMutI CAGTG 3 cut(s) 988, 1179, 1207
BtuMI TCGCGA 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 865, 1393
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 2 cut(s) 1197, 1260
CseI GACGC 1 cut(s) 153
Csp6I GTAC 3 cut(s) 696, 1125, 1442
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 8 cut(s) 244, 322, 331, 356, 524, 744, 1349, 1392
CviQI GTAC 3 cut(s) 696, 1125, 1442
DdeI CTNAG 8 cut(s) 49, 85, 130, 362, 492, 677, 831, 1407
DpnI GATC 7 cut(s) 146, 179, 320, 354, 628, 1063, 1149
DpnII GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 4 cut(s) 109, 129, 420, 1045
EarI CTCTTC 4 cut(s) 109, 129, 420, 1045
Eco130I CCWWGG 2 cut(s) 345, 1348
Eco47I GGWCC 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 1385
EcoRII CCWGG 2 cut(s) 290, 1357
EcoT14I CCWWGG 2 cut(s) 345, 1348
ErhI CCWWGG 2 cut(s) 345, 1348
FaeI CATG 8 cut(s) 247, 325, 334, 359, 527, 747, 1352, 1395
FaqI GGGAC 1 cut(s) 1190
FatI CATG 8 cut(s) 243, 321, 330, 355, 523, 743, 1348, 1391
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 3 cut(s) 240, 296, 882
Fnu4HI GCNGC 6 cut(s) 162, 795, 1000, 1092, 1355, 1428
FokI GGATG 3 cut(s) 289, 784, 1157
Fsp4HI GCNGC 6 cut(s) 162, 795, 1000, 1092, 1355, 1428
FspBI CTAG 6 cut(s) 66, 461, 803, 1245, 1445, 1496
GluI GCNGC 6 cut(s) 162, 795, 1000, 1092, 1355, 1428
HaeIII GGCC 2 cut(s) 303, 1262
HapII CCGG 1 cut(s) 300
HgaI GACGC 1 cut(s) 153
Hin1II CATG 8 cut(s) 247, 325, 334, 359, 527, 747, 1352, 1395
HincII GTYRAC 2 cut(s) 883, 1288
HindII GTYRAC 2 cut(s) 883, 1288
HindIII AAGCTT 2 cut(s) 758, 901
HpaII CCGG 1 cut(s) 300
HphI GGTGA 5 cut(s) 196, 442, 451, 508, 1004
Hpy166II GTNNAC 5 cut(s) 241, 297, 883, 1177, 1288
Hpy188III TCNNGA 6 cut(s) 20, 557, 646, 1099, 1157, 1454
Hpy8I GTNNAC 5 cut(s) 241, 297, 883, 1177, 1288
Hpy99I CGWCG 2 cut(s) 887, 1184
HpyCH4III ACNGT 4 cut(s) 457, 983, 1174, 1292
HpyCH4V TGCA 7 cut(s) 407, 726, 1071, 1283, 1295, 1395, 1427
HpyF10VI GCNNNNNNNGC 3 cut(s) 534, 1110, 1424
HpyF3I CTNAG 8 cut(s) 49, 85, 130, 362, 492, 677, 831, 1407
Hsp92II CATG 8 cut(s) 247, 325, 334, 359, 527, 747, 1352, 1395
KpnI GGTACC 1 cut(s) 699
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
LmnI GCTCC 1 cut(s) 1216
Lsp1109I GCAGC 6 cut(s) 148, 806, 1011, 1103, 1366, 1439
LweI GCATC 5 cut(s) 256, 504, 639, 806, 1439
MaeI CTAG 6 cut(s) 66, 461, 803, 1245, 1445, 1496
MaeIII GTNAC 3 cut(s) 95, 172, 850
MalI GATC 7 cut(s) 146, 179, 320, 354, 628, 1063, 1149
MboI GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
MflI RGATCY 1 cut(s) 626
MluCI AATT 5 cut(s) 618, 635, 668, 1119, 1152
MlyI GAGTC 4 cut(s) 17, 95, 119, 403
MseI TTAA 3 cut(s) 711, 905, 1217
MslI CAYNNNNRTG 4 cut(s) 66, 248, 528, 647
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 292, 1359
MvaI CCWGG 2 cut(s) 292, 1359
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 534, 1110, 1424
NcoI CCATGG 1 cut(s) 1348
NdeII GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
NlaIII CATG 8 cut(s) 247, 325, 334, 359, 527, 747, 1352, 1395
NlaIV GGNNCC 4 cut(s) 697, 741, 1261, 1323
NmuCI GTSAC 3 cut(s) 95, 172, 850
NruI TCGCGA 1 cut(s) 21
NspI RCATGY 1 cut(s) 1395
NspV TTCGAA 2 cut(s) 1047, 1117
OliI CACNNNNGTG 1 cut(s) 66
PaeI GCATGC 1 cut(s) 1395
PaeR7I CTCGAG 1 cut(s) 1385
PcsI WCGNNNNNNNCGW 1 cut(s) 864
PflFI GACNNNGTC 1 cut(s) 878
PkrI GCNGC 6 cut(s) 163, 796, 1001, 1093, 1356, 1429
Ple19I CGATCG 1 cut(s) 147
PleI GAGTC 4 cut(s) 17, 95, 119, 402
PpsI GAGTC 4 cut(s) 17, 95, 119, 402
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 290, 1357
PspGI CCWGG 2 cut(s) 290, 1357
PspN4I GGNNCC 4 cut(s) 697, 741, 1261, 1323
PspPI GGNCC 2 cut(s) 1197, 1260
PspXI VCTCGAGB 1 cut(s) 1385
PstI CTGCAG 1 cut(s) 1285
PsuI RGATCY 1 cut(s) 626
PsyI GACNNNGTC 1 cut(s) 878
PvuI CGATCG 1 cut(s) 147
PvuII CAGCTG 1 cut(s) 823
RruI TCGCGA 1 cut(s) 21
RsaI GTAC 3 cut(s) 697, 1126, 1443
RsaNI GTAC 3 cut(s) 696, 1125, 1442
RseI CAYNNNNRTG 4 cut(s) 66, 248, 528, 647
SalI GTCGAC 1 cut(s) 881
SaqAI TTAA 3 cut(s) 711, 905, 1217
SatI GCNGC 6 cut(s) 162, 795, 1000, 1092, 1355, 1428
Sau3AI GATC 7 cut(s) 144, 177, 318, 352, 626, 1061, 1147
Sau96I GGNCC 2 cut(s) 1197, 1260
ScaI AGTACT 1 cut(s) 1443
SchI GAGTC 4 cut(s) 17, 95, 119, 403
ScrFI CCNGG 2 cut(s) 292, 1359
SfaNI GCATC 5 cut(s) 256, 504, 639, 806, 1439
SfcI CTRYAG 1 cut(s) 1281
Sfr274I CTCGAG 1 cut(s) 1385
SfuI TTCGAA 2 cut(s) 1047, 1117
SinI GGWCC 1 cut(s) 1197
SlaI CTCGAG 1 cut(s) 1385
SmiMI CAYNNNNRTG 4 cut(s) 66, 248, 528, 647
SmlI CTYRAG 2 cut(s) 271, 1385
SmoI CTYRAG 2 cut(s) 271, 1385
SphI GCATGC 1 cut(s) 1395
Sse9I AATT 5 cut(s) 618, 635, 668, 1119, 1152
SspI AATATT 2 cut(s) 909, 1273
SspMI CTAG 6 cut(s) 66, 461, 803, 1245, 1445, 1496
StyD4I CCNGG 2 cut(s) 290, 1357
StyI CCWWGG 2 cut(s) 345, 1348
TaaI ACNGT 4 cut(s) 457, 983, 1174, 1292
TasI AATT 5 cut(s) 618, 635, 668, 1119, 1152
TatI WGTACW 1 cut(s) 1441
Tru1I TTAA 3 cut(s) 711, 905, 1217
Tru9I TTAA 3 cut(s) 711, 905, 1217
TscAI CASTG 3 cut(s) 988, 1179, 1207
TseFI GTSAC 3 cut(s) 95, 172, 850
TseI GCWGC 6 cut(s) 161, 794, 999, 1091, 1354, 1427
Tsp45I GTSAC 3 cut(s) 95, 172, 850
TspDTI ATGAA 7 cut(s) 17, 132, 804, 942, 1014, 1281, 1464
TspRI CASTG 3 cut(s) 988, 1179, 1207
Tth111I GACNNNGTC 1 cut(s) 878
VpaK11BI GGWCC 1 cut(s) 1197
XapI RAATTY 3 cut(s) 668, 1119, 1152
XceI RCATGY 1 cut(s) 1395
XhoI CTCGAG 1 cut(s) 1385
XmiI GTMKAC 3 cut(s) 240, 296, 882
XspI CTAG 6 cut(s) 66, 461, 803, 1245, 1445, 1496
ZrmI AGTACT 1 cut(s) 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.