Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g21010 |
| pyrus_communis | pycom05g18030 |
| rosa_chinensis | RchiOBHm_Chr6g0287481 RchiOBHm_Chr6g0287491 |
| rosa_laevigata | RLG00000012490 RLG00000012492 |
| rosa_multiflora | Rmu_sc0007029.1_g000014 Rmu_sc0007029.1_g000017 Rmu_sc0007029.1_g000018 |
| rosa_roxburghii | Rroxscaffold_7G00181430 Rroxscaffold_7G00181450 Rroxscaffold_7G00181460 Rroxscaffold_7G00181470 |
| rosa_rugosa | Rorug06G0188000.1 Rorug06G0188300 |
| rosa_samantha | Rh6AG299600 Rh6AG299700 Rh6BG304900 Rh6BG305000 Rh6BG305100 Rh6CG304400 Rh6CG304600 Rh6CG304700 Rh6CG313400 Rh6CG313500 Rh6CG313700 Rh6DG296800 Rh6DG296900 |
| rosa_wichuraiana | Rw6G025850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfeI | AGCGCT | 1 cut(s) | 176 |
| AjnI | CCWGG | 1 cut(s) | 128 |
| Aor51HI | AGCGCT | 1 cut(s) | 176 |
| ApeKI | GCWGC | 1 cut(s) | 165 |
| AseI | ATTAAT | 1 cut(s) | 159 |
| AspLEI | GCGC | 1 cut(s) | 177 |
| AsuHPI | GGTGA | 1 cut(s) | 50 |
| AsuII | TTCGAA | 1 cut(s) | 180 |
| BanII | GRGCYC | 1 cut(s) | 103 |
| BbvI | GCAGC | 1 cut(s) | 177 |
| BciT130I | CCWGG | 1 cut(s) | 130 |
| BfaI | CTAG | 1 cut(s) | 204 |
| BfoI | RGCGCY | 1 cut(s) | 178 |
| BisI | GCNGC | 1 cut(s) | 166 |
| BlsI | GCNGC | 1 cut(s) | 167 |
| Bme1390I | CCNGG | 1 cut(s) | 130 |
| BmrFI | CCNGG | 1 cut(s) | 130 |
| BpmI | CTGGAG | 1 cut(s) | 151 |
| Bpu14I | TTCGAA | 1 cut(s) | 180 |
| BseBI | CCWGG | 1 cut(s) | 130 |
| BseXI | GCAGC | 1 cut(s) | 177 |
| Bsp119I | TTCGAA | 1 cut(s) | 180 |
| Bsp1286I | GDGCHC | 1 cut(s) | 103 |
| BspT104I | TTCGAA | 1 cut(s) | 180 |
| Bst2UI | CCWGG | 1 cut(s) | 130 |
| BstBI | TTCGAA | 1 cut(s) | 180 |
| BstDEI | CTNAG | 1 cut(s) | 104 |
| BstH2I | RGCGCY | 1 cut(s) | 178 |
| BstHHI | GCGC | 1 cut(s) | 177 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 174 |
| BstNI | CCWGG | 1 cut(s) | 130 |
| BstSCI | CCNGG | 1 cut(s) | 128 |
| BstV1I | GCAGC | 1 cut(s) | 177 |
| CfoI | GCGC | 1 cut(s) | 177 |
| CviJI | RGCY | 1 cut(s) | 101 |
| CviKI_1 | RGCY | 1 cut(s) | 101 |
| DdeI | CTNAG | 1 cut(s) | 104 |
| Eco24I | GRGCYC | 1 cut(s) | 103 |
| Eco47III | AGCGCT | 1 cut(s) | 176 |
| EcoRII | CCWGG | 1 cut(s) | 128 |
| EcoT38I | GRGCYC | 1 cut(s) | 103 |
| FaiI | YATR | 4 cut(s) | 33, 59, 75, 123 |
| Fnu4HI | GCNGC | 1 cut(s) | 166 |
| FriOI | GRGCYC | 1 cut(s) | 103 |
| Fsp4HI | GCNGC | 1 cut(s) | 166 |
| FspBI | CTAG | 1 cut(s) | 204 |
| GlaI | GCGC | 1 cut(s) | 176 |
| GluI | GCNGC | 1 cut(s) | 166 |
| GsuI | CTGGAG | 1 cut(s) | 151 |
| HaeII | RGCGCY | 1 cut(s) | 178 |
| HhaI | GCGC | 1 cut(s) | 177 |
| Hin6I | GCGC | 1 cut(s) | 175 |
| HinP1I | GCGC | 1 cut(s) | 175 |
| HinfI | GANTC | 1 cut(s) | 182 |
| HphI | GGTGA | 1 cut(s) | 50 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 174 |
| HpyF3I | CTNAG | 1 cut(s) | 104 |
| HspAI | GCGC | 1 cut(s) | 175 |
| LpnPI | CCDG | 3 cut(s) | 115, 142, 185 |
| Lsp1109I | GCAGC | 1 cut(s) | 177 |
| MaeI | CTAG | 1 cut(s) | 204 |
| MhlI | GDGCHC | 1 cut(s) | 103 |
| MluCI | AATT | 1 cut(s) | 151 |
| MnlI | CCTC | 2 cut(s) | 70, 153 |
| MseI | TTAA | 2 cut(s) | 48, 159 |
| MslI | CAYNNNNRTG | 1 cut(s) | 204 |
| MspR9I | CCNGG | 1 cut(s) | 130 |
| MvaI | CCWGG | 1 cut(s) | 130 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 174 |
| NspV | TTCGAA | 1 cut(s) | 180 |
| PfeI | GAWTC | 1 cut(s) | 182 |
| PfoI | TCCNGGA | 1 cut(s) | 128 |
| PkrI | GCNGC | 1 cut(s) | 167 |
| PshBI | ATTAAT | 1 cut(s) | 159 |
| Psp6I | CCWGG | 1 cut(s) | 128 |
| PspGI | CCWGG | 1 cut(s) | 128 |
| RseI | CAYNNNNRTG | 1 cut(s) | 204 |
| SaqAI | TTAA | 2 cut(s) | 48, 159 |
| SatI | GCNGC | 1 cut(s) | 166 |
| ScrFI | CCNGG | 1 cut(s) | 130 |
| SduI | GDGCHC | 1 cut(s) | 103 |
| SetI | ASST | 1 cut(s) | 110 |
| SfuI | TTCGAA | 1 cut(s) | 180 |
| SgeI | CNNG | 6 cut(s) | 141, 142, 158, 184, 198, 202 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 204 |
| Sse9I | AATT | 1 cut(s) | 151 |
| SspMI | CTAG | 1 cut(s) | 204 |
| StyD4I | CCNGG | 1 cut(s) | 128 |
| TaqI | TCGA | 1 cut(s) | 180 |
| TasI | AATT | 1 cut(s) | 151 |
| TfiI | GAWTC | 1 cut(s) | 182 |
| Tru1I | TTAA | 2 cut(s) | 48, 159 |
| Tru9I | TTAA | 2 cut(s) | 48, 159 |
| TseI | GCWGC | 1 cut(s) | 165 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| VspI | ATTAAT | 1 cut(s) | 159 |
| XspI | CTAG | 1 cut(s) | 204 |