Rh6AG299600

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005522
Physical Location & Seq
Forward (+)
49896953 .. 49898939
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG299600.1

Sequence Viewer

Length: 1053 bp
ATGTTTGCTGACGAGTACATGGACGATTCCGTGAGGTTTTGTAATTGTGATGTGAATGAGGAAATGCCGATTTATTTACTGTCGAGAGGGAAGAAATTTCGGGTTGTTGCGAAAGGTTGTGGGTGGGAAGAATTGATTATGGTGTGTGAATCATATACTGTGGGGGATGTAAAGAAGATGGTTAAGAATTTTACTCAGGTGAAGAGTGAAGGACTGGTGCTTTTTTGGGGCGGAAATGATATGGTTCGTCTTGATGATGATGGGAAGCCCCTTGTGTTTTACGATGTAGTAGAGAAATCTACGATTTGGGTACTTGGTTTGGCTGTGAAATTGACCATCAAGATGCCACAGGCTGGAGATGTGGTGACCCTCAGTGCTTATAGGGTCAACACCGTAAAGGACATCAAGTCTTATAAATTTGAGACGATGGGGATCCCATTTAGCAGTCAGAAGCTTTTCTATAGGGGGATTTTTCTTGCTGATGAGGCTGATTCCCTAGATGGCTACAACAGGGAAGGTAATTGTGATATTTTTGCAACTTTTGGATCAGGAGATGATGATGAACAAGTTCCAGATGTTGAAAAAATAAAGTTAATTTTCAAGGGAGACTCCCATTCGCATGTGTTCAAGGTGGAATGCTCAAGTTACGAAGAATCAGAAAGCGAGTTGGATGACGAAGAGTCAGAGGAGGAAACATTTGTACTCCAGTTGAAATCTTCGAATACCATGTTGGACGTCAAGACAGCAGTTCAGAGCCTGAAGAGCTTCCCAGTTTGTTCCCAGAAGCTATACTGTCATGAAAGATTGCTGAAAGATTGCAAAAATCTTTGGGAGAGCAGTGTTGCTACAAAGTGGTTATTTGTTAGCTCTCAATTAACAATGTTCATTGAGACACCAAACCATAAGAAGGTTGTAATCAAACCTAGCAGTAGAAATGCAGTTCATAACATTTTGTACATGATTCCCAAACAAGCCCTCGACAAAATCAATTGGAAGCCAAGGCTCTTCCATGATGGTAATGTTCTCCCAGAGCACAAAACCCTGGCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

350

Amino Acids

39.96

Weight (kDa)

5.27

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AatII GACGTC 1 cut(s) 738
AccB7I CCANNNNNTGG 1 cut(s) 353
AciI CCGC 1 cut(s) 231
AclWI GGATC 3 cut(s) 427, 440, 553
AcsI RAATTY 3 cut(s) 95, 187, 416
AcuI CTGAAG 1 cut(s) 779
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 4 cut(s) 17, 312, 702, 956
AfiI CCNNNNNNNGG 2 cut(s) 353, 907
AgsI TTSAA 4 cut(s) 581, 601, 628, 712
AhdI GACNNNNNGTC 2 cut(s) 406, 679
AjnI CCWGG 1 cut(s) 1041
AjuI GAANNNNNNNTTGG 2 cut(s) 713, 745
AluBI AGCT 4 cut(s) 454, 765, 787, 867
AluI AGCT 4 cut(s) 454, 765, 787, 867
Alw21I GWGCWC 1 cut(s) 1035
Alw26I GTCTC 3 cut(s) 416, 600, 884
AlwI GGATC 3 cut(s) 427, 440, 553
AlwNI CAGNNNCTG 1 cut(s) 757
ApoI RAATTY 3 cut(s) 95, 187, 416
Asp700I GAANNNNTTC 3 cut(s) 455, 567, 764
AsuHPI GGTGA 2 cut(s) 211, 376
AsuII TTCGAA 1 cut(s) 719
BamHI GGATCC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 1035
BccI CCATC 6 cut(s) 172, 254, 344, 421, 494, 1007
BciT130I CCWGG 1 cut(s) 1043
BcoDI GTCTC 3 cut(s) 416, 600, 884
BfaI CTAG 2 cut(s) 497, 924
BfmI CTRYAG 1 cut(s) 460
Bme1390I CCNGG 1 cut(s) 1043
BmeRI GACNNNNNGTC 2 cut(s) 406, 679
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 1 cut(s) 1043
BmrI ACTGGG 1 cut(s) 764
BmsI GCATC 1 cut(s) 333
BmuI ACTGGG 1 cut(s) 764
BpmI CTGGAG 2 cut(s) 375, 689
Bpu14I TTCGAA 1 cut(s) 719
BpuEI CTTGAG 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 431
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 2 cut(s) 998, 1041
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 907
Bse1I ACTGG 3 cut(s) 219, 706, 770
Bse8I GATNNNNATC 1 cut(s) 431
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 2 cut(s) 998, 1041
BseGI GGATG 2 cut(s) 172, 676
BseJI GATNNNNATC 1 cut(s) 431
BseLI CCNNNNNNNGG 2 cut(s) 353, 907
BseMII CTCAG 2 cut(s) 209, 385
BseNI ACTGG 3 cut(s) 219, 706, 770
BseRI GAGGAG 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 1035
BslI CCNNNNNNNGG 2 cut(s) 353, 907
BsmAI GTCTC 3 cut(s) 416, 600, 884
BsmBI CGTCTC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 1035
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 2 cut(s) 432, 545
BspACI CCGC 1 cut(s) 231
BspCNI CTCAG 2 cut(s) 208, 384
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 434
BspPI GGATC 3 cut(s) 427, 440, 553
BspQI GCTCTTC 2 cut(s) 755, 1010
BspT104I TTCGAA 1 cut(s) 719
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 3 cut(s) 219, 706, 770
BssECI CCNNGG 2 cut(s) 998, 1041
BssMI GATC 2 cut(s) 432, 545
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 1 cut(s) 998
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 4 cut(s) 81, 160, 394, 794
Bst6I CTCTTC 4 cut(s) 197, 672, 755, 1010
BstACI GRCGYC 1 cut(s) 735
BstAUI TGTACA 1 cut(s) 954
BstBI TTCGAA 1 cut(s) 719
BstDEI CTNAG 3 cut(s) 195, 371, 1047
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 2 cut(s) 172, 676
BstKTI GATC 2 cut(s) 435, 548
BstMAI GTCTC 3 cut(s) 416, 600, 884
BstMBI GATC 2 cut(s) 432, 545
BstMWI GCNNNNNNNGC 2 cut(s) 485, 762
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 1 cut(s) 623
BstPI GGTNACC 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 1041
BstSFI CTRYAG 1 cut(s) 460
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
BtsCI GGATG 2 cut(s) 172, 676
BtsI GCAGTG 1 cut(s) 844
BtsIMutI CAGTG 2 cut(s) 379, 844
CaiI CAGNNNCTG 1 cut(s) 757
CciI TCATGA 1 cut(s) 796
Csp6I GTAC 4 cut(s) 16, 311, 701, 955
CviAII CATG 6 cut(s) 19, 620, 727, 797, 958, 1010
CviQI GTAC 4 cut(s) 16, 311, 701, 955
DdeI CTNAG 3 cut(s) 195, 371, 1047
DpnI GATC 2 cut(s) 434, 547
DpnII GATC 2 cut(s) 432, 545
DriI GACNNNNNGTC 2 cut(s) 406, 679
Eam1104I CTCTTC 4 cut(s) 197, 672, 755, 1010
Eam1105I GACNNNNNGTC 2 cut(s) 406, 679
EarI CTCTTC 4 cut(s) 197, 672, 755, 1010
EciI GGCGGA 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 998
Eco57I CTGAAG 1 cut(s) 779
Eco91I GGTNACC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 1041
EcoT14I CCWWGG 1 cut(s) 998
ErhI CCWWGG 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 6 cut(s) 22, 623, 730, 800, 961, 1013
FatI CATG 6 cut(s) 18, 619, 726, 796, 957, 1009
FokI GGATG 2 cut(s) 179, 683
FspBI CTAG 2 cut(s) 497, 924
GsuI CTGGAG 2 cut(s) 375, 689
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 6 cut(s) 22, 623, 730, 800, 961, 1013
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 7 cut(s) 26, 149, 491, 608, 653, 680, 961
HphI GGTGA 2 cut(s) 211, 376
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 4 cut(s) 450, 658, 685, 753
Hpy188III TCNNGA 7 cut(s) 84, 251, 340, 549, 572, 739, 797
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 3 cut(s) 203, 509, 901
HpyCH4III ACNGT 4 cut(s) 81, 160, 394, 794
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 3 cut(s) 536, 819, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 762
HpyF3I CTNAG 3 cut(s) 195, 371, 1047
HpySE526I ACGT 1 cut(s) 735
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 6 cut(s) 22, 623, 730, 800, 961, 1013
Kzo9I GATC 2 cut(s) 432, 545
LguI GCTCTTC 2 cut(s) 755, 1010
LweI GCATC 1 cut(s) 333
MaeI CTAG 2 cut(s) 497, 924
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 2 cut(s) 364, 644
MalI GATC 2 cut(s) 434, 547
MboI GATC 2 cut(s) 432, 545
MboII GAAGA 9 cut(s) 103, 140, 187, 214, 662, 689, 708, 772, 997
MfeI CAATTG 1 cut(s) 988
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 1035
MlyI GAGTC 2 cut(s) 602, 689
MmeI TCCRAC 2 cut(s) 648, 711
MnlI CCTC 8 cut(s) 27, 52, 80, 380, 478, 679, 682, 986
MroXI GAANNNNTTC 3 cut(s) 455, 567, 764
MseI TTAA 3 cut(s) 183, 593, 875
MslI CAYNNNNRTG 2 cut(s) 341, 618
MspR9I CCNGG 1 cut(s) 1043
MunI CAATTG 1 cut(s) 988
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 1 cut(s) 1043
MwoI GCNNNNNNNGC 2 cut(s) 485, 762
NdeII GATC 2 cut(s) 432, 545
NlaIII CATG 6 cut(s) 22, 623, 730, 800, 961, 1013
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 364
NspI RCATGY 1 cut(s) 623
NspV TTCGAA 1 cut(s) 719
PagI TCATGA 1 cut(s) 796
PciSI GCTCTTC 2 cut(s) 755, 1010
PctI GAATGC 1 cut(s) 641
PdmI GAANNNNTTC 3 cut(s) 455, 567, 764
PfeI GAWTC 5 cut(s) 26, 149, 491, 653, 961
PflMI CCANNNNNTGG 1 cut(s) 353
PleI GAGTC 2 cut(s) 602, 688
PpsI GAGTC 2 cut(s) 602, 688
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 1041
PspEI GGTNACC 1 cut(s) 364
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 1 cut(s) 434
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 1 cut(s) 432
RsaI GTAC 4 cut(s) 17, 312, 702, 956
RsaNI GTAC 4 cut(s) 16, 311, 701, 955
RseI CAYNNNNRTG 2 cut(s) 341, 618
SapI GCTCTTC 2 cut(s) 755, 1010
SaqAI TTAA 3 cut(s) 183, 593, 875
Sau3AI GATC 2 cut(s) 432, 545
SchI GAGTC 2 cut(s) 602, 689
ScrFI CCNGG 1 cut(s) 1043
SduI GDGCHC 1 cut(s) 1035
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 719
SmiMI CAYNNNNRTG 2 cut(s) 341, 618
SmlI CTYRAG 1 cut(s) 640
SmoI CTYRAG 1 cut(s) 640
SsiI CCGC 1 cut(s) 231
SspMI CTAG 2 cut(s) 497, 924
StyD4I CCNGG 1 cut(s) 1041
StyI CCWWGG 1 cut(s) 998
TaaI ACNGT 4 cut(s) 81, 160, 394, 794
TaiI ACGT 1 cut(s) 738
TaqI TCGA 3 cut(s) 83, 719, 978
TatI WGTACW 3 cut(s) 15, 700, 954
TfiI GAWTC 5 cut(s) 26, 149, 491, 653, 961
Tru1I TTAA 3 cut(s) 183, 593, 875
Tru9I TTAA 3 cut(s) 183, 593, 875
TscAI CASTG 2 cut(s) 379, 844
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 4 cut(s) 576, 813, 874, 932
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 2 cut(s) 379, 844
Van91I CCANNNNNTGG 1 cut(s) 353
XapI RAATTY 3 cut(s) 95, 187, 416
XceI RCATGY 1 cut(s) 623
XmnI GAANNNNTTC 3 cut(s) 455, 567, 764
XspI CTAG 2 cut(s) 497, 924
ZraI GACGTC 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.