Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g21010 |
| pyrus_communis | pycom05g18030 |
| rosa_chinensis | RchiOBHm_Chr6g0287481 RchiOBHm_Chr6g0287491 |
| rosa_laevigata | RLG00000012490 RLG00000012492 |
| rosa_multiflora | Rmu_sc0007029.1_g000014 Rmu_sc0007029.1_g000017 Rmu_sc0007029.1_g000018 |
| rosa_roxburghii | Rroxscaffold_7G00181430 Rroxscaffold_7G00181450 Rroxscaffold_7G00181460 Rroxscaffold_7G00181470 |
| rosa_rugosa | Rorug06G0188000.1 Rorug06G0188300 |
| rosa_samantha | Rh6AG299600 Rh6AG299700 Rh6BG304900 Rh6BG305000 Rh6BG305100 Rh6CG304400 Rh6CG304600 Rh6CG304700 Rh6CG313400 Rh6CG313500 Rh6CG313700 Rh6DG296800 Rh6DG296900 |
| rosa_wichuraiana | Rw6G025850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1218 |
| Acc65I | GGTACC | 1 cut(s) | 953 |
| AccB1I | GGYRCC | 1 cut(s) | 953 |
| AccI | GTMKAC | 3 cut(s) | 420, 476, 1140 |
| AccII | CGCG | 1 cut(s) | 21 |
| AciI | CCGC | 3 cut(s) | 241, 1577, 1607 |
| AclWI | GGATC | 1 cut(s) | 604 |
| AcoI | YGGCCR | 1 cut(s) | 481 |
| AcsI | RAATTY | 5 cut(s) | 926, 1371, 1404, 1763, 1912 |
| AcuI | CTGAAG | 1 cut(s) | 612 |
| AcyI | GRCGYC | 2 cut(s) | 8, 273 |
| AfaI | GTAC | 3 cut(s) | 955, 1378, 1924 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 410, 619, 917 |
| AgsI | TTSAA | 8 cut(s) | 214, 740, 1072, 1202, 1562, 1667, 1768, 1790 |
| AjnI | CCWGG | 3 cut(s) | 470, 1609, 1928 |
| AluBI | AGCT | 9 cut(s) | 559, 863, 939, 1018, 1081, 1088, 1161, 1387, 1670 |
| AluI | AGCT | 9 cut(s) | 559, 863, 939, 1018, 1081, 1088, 1161, 1387, 1670 |
| Alw21I | GWGCWC | 1 cut(s) | 318 |
| Alw26I | GTCTC | 3 cut(s) | 1170, 1478, 1816 |
| AlwI | GGATC | 1 cut(s) | 604 |
| AoxI | GGCC | 4 cut(s) | 481, 1512, 1604, 1718 |
| ApeKI | GCWGC | 7 cut(s) | 338, 597, 600, 1052, 1257, 1343, 1962 |
| ApoI | RAATTY | 5 cut(s) | 926, 1371, 1404, 1763, 1912 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 209 |
| Asp718I | GGTACC | 1 cut(s) | 953 |
| AspLEI | GCGC | 1 cut(s) | 288 |
| AspS9I | GGNCC | 4 cut(s) | 360, 622, 1449, 1604 |
| AsuHPI | GGTGA | 6 cut(s) | 187, 700, 709, 766, 1262, 1850 |
| AsuII | TTCGAA | 2 cut(s) | 1299, 1369 |
| AvaII | GGWCC | 3 cut(s) | 360, 622, 1449 |
| BanI | GGYRCC | 1 cut(s) | 953 |
| BauI | CACGAG | 1 cut(s) | 666 |
| BbsI | GAAGAC | 2 cut(s) | 11, 1569 |
| Bbv12I | GWGCWC | 1 cut(s) | 318 |
| BbvCI | CCTCAGC | 2 cut(s) | 542, 750 |
| BbvI | GCAGC | 6 cut(s) | 350, 609, 612, 1064, 1269, 1355 |
| BccI | CCATC | 9 cut(s) | 242, 451, 727, 799, 908, 985, 1619, 1829, 1954 |
| BceAI | ACGGC | 1 cut(s) | 115 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 298, 332, 1312, 1346 |
| BciT130I | CCWGG | 3 cut(s) | 472, 1611, 1930 |
| BclI | TGATCA | 2 cut(s) | 255, 1399 |
| BcoDI | GTCTC | 3 cut(s) | 1170, 1478, 1816 |
| BfaI | CTAG | 9 cut(s) | 66, 296, 335, 719, 1061, 1497, 1503, 1716, 1793 |
| BfmI | CTRYAG | 2 cut(s) | 1533, 1863 |
| BfoI | RGCGCY | 1 cut(s) | 289 |
| BglII | AGATCT | 1 cut(s) | 884 |
| BisI | GCNGC | 8 cut(s) | 339, 598, 601, 1053, 1258, 1344, 1607, 1963 |
| BlpI | GCTNAGC | 1 cut(s) | 935 |
| BlsI | GCNGC | 8 cut(s) | 340, 599, 602, 1054, 1259, 1345, 1608, 1964 |
| Bme1390I | CCNGG | 3 cut(s) | 472, 1611, 1930 |
| Bme18I | GGWCC | 3 cut(s) | 360, 622, 1449 |
| BmgT120I | GGNCC | 4 cut(s) | 360, 622, 1449, 1604 |
| BmiI | GGNNCC | 2 cut(s) | 955, 1575 |
| BmrFI | CCNGG | 3 cut(s) | 472, 1611, 1930 |
| BmrI | ACTGGG | 2 cut(s) | 1402, 1485 |
| BmsI | GCATC | 6 cut(s) | 436, 762, 897, 1064, 1731, 1889 |
| BmuI | ACTGGG | 2 cut(s) | 1402, 1485 |
| BpiI | GAAGAC | 2 cut(s) | 11, 1569 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1813, 1845 |
| Bpu10I | CCTNAGC | 2 cut(s) | 542, 750 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 935 |
| Bpu14I | TTCGAA | 2 cut(s) | 1299, 1369 |
| BpuEI | CTTGAG | 3 cut(s) | 230, 472, 1502 |
| BsaAI | YACGTR | 1 cut(s) | 983 |
| BsaHI | GRCGYC | 2 cut(s) | 8, 273 |
| BsaJI | CCNNGG | 3 cut(s) | 470, 525, 1600 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 410, 619, 917 |
| Bse118I | RCCGGY | 1 cut(s) | 479 |
| Bse1I | ACTGG | 4 cut(s) | 1397, 1452, 1491, 1975 |
| Bse3DI | GCAATG | 2 cut(s) | 793, 1649 |
| BseBI | CCWGG | 3 cut(s) | 472, 1611, 1930 |
| BseDI | CCNNGG | 3 cut(s) | 470, 525, 1600 |
| BseGI | GGATG | 5 cut(s) | 462, 1055, 1402, 1435, 1777 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 410, 619, 917 |
| BseMI | GCAATG | 2 cut(s) | 793, 1649 |
| BseMII | CTCAG | 7 cut(s) | 63, 99, 144, 556, 741, 1080, 1673 |
| BseNI | ACTGG | 4 cut(s) | 1397, 1452, 1491, 1975 |
| BseRI | GAGGAG | 3 cut(s) | 73, 118, 212 |
| BseXI | GCAGC | 6 cut(s) | 350, 609, 612, 1064, 1269, 1355 |
| BsgI | GTGCAG | 3 cut(s) | 648, 1566, 1721 |
| Bsh1236I | CGCG | 1 cut(s) | 21 |
| Bsh1285I | CGRYCG | 1 cut(s) | 147 |
| BshFI | GGCC | 4 cut(s) | 483, 1514, 1606, 1720 |
| BshNI | GGYRCC | 1 cut(s) | 953 |
| BsiEI | CGRYCG | 1 cut(s) | 147 |
| BsiHKAI | GWGCWC | 1 cut(s) | 318 |
| BsiSI | CCGG | 1 cut(s) | 480 |
| BslFI | GGGAC | 2 cut(s) | 260, 1442 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 410, 619, 917 |
| BsmAI | GTCTC | 3 cut(s) | 1170, 1478, 1816 |
| BsmFI | GGGAC | 2 cut(s) | 260, 1442 |
| BsmI | GAATGC | 1 cut(s) | 1365 |
| BsnI | GGCC | 4 cut(s) | 483, 1514, 1606, 1720 |
| Bsp119I | TTCGAA | 2 cut(s) | 1299, 1369 |
| Bsp1286I | GDGCHC | 1 cut(s) | 318 |
| Bsp143I | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 935 |
| Bsp19I | CCATGG | 1 cut(s) | 1600 |
| Bsp68I | TCGCGA | 1 cut(s) | 21 |
| BspACI | CCGC | 3 cut(s) | 241, 1577, 1607 |
| BspANI | GGCC | 4 cut(s) | 483, 1514, 1606, 1720 |
| BspCNI | CTCAG | 7 cut(s) | 62, 98, 143, 555, 742, 1081, 1672 |
| BspFNI | CGCG | 1 cut(s) | 21 |
| BspHI | TCATGA | 1 cut(s) | 1831 |
| BspLI | GGNNCC | 2 cut(s) | 955, 1575 |
| BspMAI | CTGCAG | 1 cut(s) | 1537 |
| BspPI | GGATC | 1 cut(s) | 604 |
| BspQI | GCTCTTC | 1 cut(s) | 109 |
| BspT104I | TTCGAA | 2 cut(s) | 1299, 1369 |
| BspT107I | GGYRCC | 1 cut(s) | 953 |
| BsrDI | GCAATG | 2 cut(s) | 793, 1649 |
| BsrFI | RCCGGY | 1 cut(s) | 479 |
| BsrI | ACTGG | 4 cut(s) | 1397, 1452, 1491, 1975 |
| BssAI | RCCGGY | 1 cut(s) | 479 |
| BssECI | CCNNGG | 3 cut(s) | 470, 525, 1600 |
| BssMI | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| BssNAI | GTATAC | 1 cut(s) | 477 |
| BssNI | GRCGYC | 2 cut(s) | 8, 273 |
| BssSI | CACGAG | 1 cut(s) | 666 |
| BssT1I | CCWWGG | 2 cut(s) | 525, 1600 |
| Bst1107I | GTATAC | 1 cut(s) | 477 |
| Bst2BI | CACGAG | 1 cut(s) | 666 |
| Bst2UI | CCWGG | 3 cut(s) | 472, 1611, 1930 |
| Bst4CI | ACNGT | 6 cut(s) | 645, 715, 1241, 1426, 1544, 1694 |
| Bst6I | CTCTTC | 3 cut(s) | 109, 129, 678 |
| BstACI | GRCGYC | 2 cut(s) | 8, 273 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1585 |
| BstBAI | YACGTR | 1 cut(s) | 983 |
| BstBI | TTCGAA | 2 cut(s) | 1299, 1369 |
| BstC8I | GCNNGC | 1 cut(s) | 1123 |
| BstDEI | CTNAG | 8 cut(s) | 49, 85, 130, 542, 750, 935, 1089, 1659 |
| BstDSI | CCRYGG | 1 cut(s) | 1600 |
| BstF5I | GGATG | 5 cut(s) | 462, 1055, 1402, 1435, 1777 |
| BstFNI | CGCG | 1 cut(s) | 21 |
| BstH2I | RGCGCY | 1 cut(s) | 289 |
| BstHHI | GCGC | 1 cut(s) | 288 |
| BstKTI | GATC | 9 cut(s) | 147, 171, 258, 501, 535, 612, 887, 1316, 1402 |
| BstMAI | GTCTC | 3 cut(s) | 1170, 1478, 1816 |
| BstMBI | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| BstMCI | CGRYCG | 1 cut(s) | 147 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 594, 792, 1362, 1585, 1971 |
| BstNI | CCWGG | 3 cut(s) | 472, 1611, 1930 |
| BstNSI | RCATGY | 2 cut(s) | 345, 1748 |
| BstSCI | CCNGG | 3 cut(s) | 470, 1609, 1928 |
| BstSFI | CTRYAG | 2 cut(s) | 1533, 1863 |
| BstSNI | TACGTA | 1 cut(s) | 983 |
| BstUI | CGCG | 1 cut(s) | 21 |
| BstV1I | GCAGC | 6 cut(s) | 350, 609, 612, 1064, 1269, 1355 |
| BstV2I | GAAGAC | 2 cut(s) | 11, 1569 |
| BstX2I | RGATCY | 1 cut(s) | 884 |
| BstYI | RGATCY | 1 cut(s) | 884 |
| BstZ17I | GTATAC | 1 cut(s) | 477 |
| BsuRI | GGCC | 4 cut(s) | 483, 1514, 1606, 1720 |
| BtgI | CCRYGG | 1 cut(s) | 1600 |
| BtgZI | GCGATG | 2 cut(s) | 810, 1140 |
| BtsCI | GGATG | 5 cut(s) | 462, 1055, 1402, 1435, 1777 |
| BtsIMutI | CAGTG | 5 cut(s) | 588, 1246, 1431, 1459, 1699 |
| BtuMI | TCGCGA | 1 cut(s) | 21 |
| Cac8I | GCNNGC | 1 cut(s) | 1123 |
| CciI | TCATGA | 1 cut(s) | 1831 |
| CfoI | GCGC | 1 cut(s) | 288 |
| Cfr10I | RCCGGY | 1 cut(s) | 479 |
| Cfr13I | GGNCC | 4 cut(s) | 360, 622, 1449, 1604 |
| CseI | GACGC | 4 cut(s) | 16, 144, 262, 1649 |
| Csp6I | GTAC | 3 cut(s) | 954, 1377, 1923 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 394, 429 |
| CviQI | GTAC | 3 cut(s) | 954, 1377, 1923 |
| DdeI | CTNAG | 8 cut(s) | 49, 85, 130, 542, 750, 935, 1089, 1659 |
| DpnI | GATC | 9 cut(s) | 146, 170, 257, 500, 534, 611, 886, 1315, 1401 |
| DpnII | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| EaeI | YGGCCR | 1 cut(s) | 481 |
| Eam1104I | CTCTTC | 3 cut(s) | 109, 129, 678 |
| EarI | CTCTTC | 3 cut(s) | 109, 129, 678 |
| Eco105I | TACGTA | 1 cut(s) | 983 |
| Eco130I | CCWWGG | 2 cut(s) | 525, 1600 |
| Eco47I | GGWCC | 3 cut(s) | 360, 622, 1449 |
| Eco57I | CTGAAG | 1 cut(s) | 612 |
| EcoRII | CCWGG | 3 cut(s) | 470, 1609, 1928 |
| EcoT14I | CCWWGG | 2 cut(s) | 525, 1600 |
| EcoT22I | ATGCAT | 1 cut(s) | 1746 |
| ErhI | CCWWGG | 2 cut(s) | 525, 1600 |
| FaqI | GGGAC | 2 cut(s) | 260, 1442 |
| FauI | CCCGC | 1 cut(s) | 248 |
| FauNDI | CATATG | 1 cut(s) | 1810 |
| FbaI | TGATCA | 2 cut(s) | 255, 1399 |
| FblI | GTMKAC | 3 cut(s) | 420, 476, 1140 |
| Fnu4HI | GCNGC | 8 cut(s) | 339, 598, 601, 1053, 1258, 1344, 1607, 1963 |
| FokI | GGATG | 5 cut(s) | 469, 1042, 1409, 1442, 1784 |
| Fsp4HI | GCNGC | 8 cut(s) | 339, 598, 601, 1053, 1258, 1344, 1607, 1963 |
| FspBI | CTAG | 9 cut(s) | 66, 296, 335, 719, 1061, 1497, 1503, 1716, 1793 |
| GlaI | GCGC | 1 cut(s) | 287 |
| GluI | GCNGC | 8 cut(s) | 339, 598, 601, 1053, 1258, 1344, 1607, 1963 |
| HaeII | RGCGCY | 1 cut(s) | 289 |
| HaeIII | GGCC | 4 cut(s) | 483, 1514, 1606, 1720 |
| HapII | CCGG | 1 cut(s) | 480 |
| HgaI | GACGC | 4 cut(s) | 16, 144, 262, 1649 |
| HhaI | GCGC | 1 cut(s) | 288 |
| Hin1I | GRCGYC | 2 cut(s) | 8, 273 |
| Hin6I | GCGC | 1 cut(s) | 286 |
| HinP1I | GCGC | 1 cut(s) | 286 |
| HincII | GTYRAC | 2 cut(s) | 1141, 1540 |
| HindII | GTYRAC | 2 cut(s) | 1141, 1540 |
| HindIII | AAGCTT | 2 cut(s) | 1016, 1159 |
| HpaII | CCGG | 1 cut(s) | 480 |
| HphI | GGTGA | 6 cut(s) | 187, 700, 709, 766, 1262, 1850 |
| Hpy166II | GTNNAC | 7 cut(s) | 301, 421, 477, 1141, 1540, 1750, 1817 |
| Hpy188III | TCNNGA | 8 cut(s) | 20, 247, 326, 655, 904, 1351, 1409, 1832 |
| Hpy8I | GTNNAC | 7 cut(s) | 301, 421, 477, 1141, 1540, 1750, 1817 |
| Hpy99I | CGWCG | 2 cut(s) | 1145, 1643 |
| HpyAV | CCTTC | 2 cut(s) | 587, 1764 |
| HpyCH4III | ACNGT | 6 cut(s) | 645, 715, 1241, 1426, 1544, 1694 |
| HpyCH4IV | ACGT | 2 cut(s) | 982, 1566 |
| HpyCH4V | TGCA | 8 cut(s) | 665, 1323, 1535, 1547, 1702, 1730, 1744, 1962 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 594, 792, 1362, 1585, 1971 |
| HpyF3I | CTNAG | 8 cut(s) | 49, 85, 130, 542, 750, 935, 1089, 1659 |
| HpySE526I | ACGT | 2 cut(s) | 982, 1566 |
| Hsp92I | GRCGYC | 2 cut(s) | 8, 273 |
| HspAI | GCGC | 1 cut(s) | 286 |
| KpnI | GGTACC | 1 cut(s) | 957 |
| Ksp22I | TGATCA | 2 cut(s) | 255, 1399 |
| Kzo9I | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| LguI | GCTCTTC | 1 cut(s) | 109 |
| LmnI | GCTCC | 1 cut(s) | 1468 |
| Lsp1109I | GCAGC | 6 cut(s) | 350, 609, 612, 1064, 1269, 1355 |
| LweI | GCATC | 6 cut(s) | 436, 762, 897, 1064, 1731, 1889 |
| MaeI | CTAG | 9 cut(s) | 66, 296, 335, 719, 1061, 1497, 1503, 1716, 1793 |
| MaeII | ACGT | 2 cut(s) | 982, 1566 |
| MaeIII | GTNAC | 4 cut(s) | 95, 163, 1108, 1711 |
| MalI | GATC | 9 cut(s) | 146, 170, 257, 500, 534, 611, 886, 1315, 1401 |
| MboI | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| MflI | RGATCY | 1 cut(s) | 884 |
| MhlI | GDGCHC | 1 cut(s) | 318 |
| MluCI | AATT | 9 cut(s) | 393, 876, 893, 926, 1371, 1404, 1763, 1901, 1912 |
| MlyI | GAGTC | 4 cut(s) | 17, 56, 119, 583 |
| Mph1103I | ATGCAT | 1 cut(s) | 1746 |
| MroXI | GAANNNNTTC | 1 cut(s) | 209 |
| MseI | TTAA | 7 cut(s) | 584, 969, 1163, 1469, 1688, 1848, 1904 |
| MslI | CAYNNNNRTG | 5 cut(s) | 428, 786, 905, 1739, 1836 |
| MspA1I | CMGCKG | 1 cut(s) | 1081 |
| MspI | CCGG | 1 cut(s) | 480 |
| MspR9I | CCNGG | 3 cut(s) | 472, 1611, 1930 |
| Mva1269I | GAATGC | 1 cut(s) | 1365 |
| MvaI | CCWGG | 3 cut(s) | 472, 1611, 1930 |
| MvnI | CGCG | 1 cut(s) | 21 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 594, 792, 1362, 1585, 1971 |
| NcoI | CCATGG | 1 cut(s) | 1600 |
| NdeI | CATATG | 1 cut(s) | 1810 |
| NdeII | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| NlaIV | GGNNCC | 2 cut(s) | 955, 1575 |
| NmuCI | GTSAC | 3 cut(s) | 95, 163, 1108 |
| NruI | TCGCGA | 1 cut(s) | 21 |
| NsiI | ATGCAT | 1 cut(s) | 1746 |
| NspI | RCATGY | 2 cut(s) | 345, 1748 |
| NspV | TTCGAA | 2 cut(s) | 1299, 1369 |
| PagI | TCATGA | 1 cut(s) | 1831 |
| PciSI | GCTCTTC | 1 cut(s) | 109 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 324, 1122 |
| PctI | GAATGC | 1 cut(s) | 1365 |
| PdmI | GAANNNNTTC | 1 cut(s) | 209 |
| PflFI | GACNNNGTC | 2 cut(s) | 947, 1136 |
| PkrI | GCNGC | 8 cut(s) | 340, 599, 602, 1054, 1259, 1345, 1608, 1964 |
| Ple19I | CGATCG | 1 cut(s) | 147 |
| PleI | GAGTC | 4 cut(s) | 17, 56, 119, 582 |
| PpsI | GAGTC | 4 cut(s) | 17, 56, 119, 582 |
| Ppu21I | YACGTR | 1 cut(s) | 983 |
| PsiI | TTATAA | 1 cut(s) | 1218 |
| Psp6I | CCWGG | 3 cut(s) | 470, 1609, 1928 |
| PspGI | CCWGG | 3 cut(s) | 470, 1609, 1928 |
| PspN4I | GGNNCC | 2 cut(s) | 955, 1575 |
| PspPI | GGNCC | 4 cut(s) | 360, 622, 1449, 1604 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1742, 1774 |
| PstI | CTGCAG | 1 cut(s) | 1537 |
| PsuI | RGATCY | 1 cut(s) | 884 |
| PsyI | GACNNNGTC | 2 cut(s) | 947, 1136 |
| PvuI | CGATCG | 1 cut(s) | 147 |
| PvuII | CAGCTG | 1 cut(s) | 1081 |
| RruI | TCGCGA | 1 cut(s) | 21 |
| RsaI | GTAC | 3 cut(s) | 955, 1378, 1924 |
| RsaNI | GTAC | 3 cut(s) | 954, 1377, 1923 |
| RseI | CAYNNNNRTG | 5 cut(s) | 428, 786, 905, 1739, 1836 |
| SalI | GTCGAC | 1 cut(s) | 1139 |
| SapI | GCTCTTC | 1 cut(s) | 109 |
| SaqAI | TTAA | 7 cut(s) | 584, 969, 1163, 1469, 1688, 1848, 1904 |
| SatI | GCNGC | 8 cut(s) | 339, 598, 601, 1053, 1258, 1344, 1607, 1963 |
| Sau3AI | GATC | 9 cut(s) | 144, 168, 255, 498, 532, 609, 884, 1313, 1399 |
| Sau96I | GGNCC | 4 cut(s) | 360, 622, 1449, 1604 |
| SchI | GAGTC | 4 cut(s) | 17, 56, 119, 583 |
| ScrFI | CCNGG | 3 cut(s) | 472, 1611, 1930 |
| SduI | GDGCHC | 1 cut(s) | 318 |
| SfaNI | GCATC | 6 cut(s) | 436, 762, 897, 1064, 1731, 1889 |
| SfcI | CTRYAG | 2 cut(s) | 1533, 1863 |
| SfuI | TTCGAA | 2 cut(s) | 1299, 1369 |
| SinI | GGWCC | 3 cut(s) | 360, 622, 1449 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 428, 786, 905, 1739, 1836 |
| SmlI | CTYRAG | 3 cut(s) | 245, 451, 1481 |
| SmoI | CTYRAG | 3 cut(s) | 245, 451, 1481 |
| SnaBI | TACGTA | 1 cut(s) | 983 |
| Sse9I | AATT | 9 cut(s) | 393, 876, 893, 926, 1371, 1404, 1763, 1901, 1912 |
| SsiI | CCGC | 3 cut(s) | 241, 1577, 1607 |
| SspI | AATATT | 1 cut(s) | 1167 |
| SspMI | CTAG | 9 cut(s) | 66, 296, 335, 719, 1061, 1497, 1503, 1716, 1793 |
| StyD4I | CCNGG | 3 cut(s) | 470, 1609, 1928 |
| StyI | CCWWGG | 2 cut(s) | 525, 1600 |
| TaaI | ACNGT | 6 cut(s) | 645, 715, 1241, 1426, 1544, 1694 |
| TaiI | ACGT | 2 cut(s) | 985, 1569 |
| TasI | AATT | 9 cut(s) | 393, 876, 893, 926, 1371, 1404, 1763, 1901, 1912 |
| TauI | GCSGC | 1 cut(s) | 1609 |
| Tru1I | TTAA | 7 cut(s) | 584, 969, 1163, 1469, 1688, 1848, 1904 |
| Tru9I | TTAA | 7 cut(s) | 584, 969, 1163, 1469, 1688, 1848, 1904 |
| TscAI | CASTG | 5 cut(s) | 595, 1246, 1431, 1459, 1699 |
| TseFI | GTSAC | 3 cut(s) | 95, 163, 1108 |
| TseI | GCWGC | 7 cut(s) | 338, 597, 600, 1052, 1257, 1343, 1962 |
| Tsp45I | GTSAC | 3 cut(s) | 95, 163, 1108 |
| TspDTI | ATGAA | 5 cut(s) | 17, 132, 1062, 1200, 1857 |
| TspRI | CASTG | 5 cut(s) | 595, 1246, 1431, 1459, 1699 |
| Tth111I | GACNNNGTC | 2 cut(s) | 947, 1136 |
| VpaK11BI | GGWCC | 3 cut(s) | 360, 622, 1449 |
| XapI | RAATTY | 5 cut(s) | 926, 1371, 1404, 1763, 1912 |
| XceI | RCATGY | 2 cut(s) | 345, 1748 |
| XmiI | GTMKAC | 3 cut(s) | 420, 476, 1140 |
| XmnI | GAANNNNTTC | 1 cut(s) | 209 |
| XspI | CTAG | 9 cut(s) | 66, 296, 335, 719, 1061, 1497, 1503, 1716, 1793 |
| Zsp2I | ATGCAT | 1 cut(s) | 1746 |