Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_2g21010 |
| pyrus_communis | pycom05g18030 |
| rosa_chinensis | RchiOBHm_Chr6g0287481 RchiOBHm_Chr6g0287491 |
| rosa_laevigata | RLG00000012490 RLG00000012492 |
| rosa_multiflora | Rmu_sc0007029.1_g000014 Rmu_sc0007029.1_g000017 Rmu_sc0007029.1_g000018 |
| rosa_roxburghii | Rroxscaffold_7G00181430 Rroxscaffold_7G00181450 Rroxscaffold_7G00181460 Rroxscaffold_7G00181470 |
| rosa_rugosa | Rorug06G0188000.1 Rorug06G0188300 |
| rosa_samantha | Rh6AG299600 Rh6AG299700 Rh6BG304900 Rh6BG305000 Rh6BG305100 Rh6CG304400 Rh6CG304600 Rh6CG304700 Rh6CG313400 Rh6CG313500 Rh6CG313700 Rh6DG296800 Rh6DG296900 |
| rosa_wichuraiana | Rw6G025850 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 41 |
| AcsI | RAATTY | 2 cut(s) | 182, 274 |
| AjnI | CCWGG | 1 cut(s) | 282 |
| ApeKI | GCWGC | 1 cut(s) | 10 |
| ApoI | RAATTY | 2 cut(s) | 182, 274 |
| AsuHPI | GGTGA | 3 cut(s) | 55, 97, 298 |
| BbvI | GCAGC | 1 cut(s) | 22 |
| BccI | CCATC | 2 cut(s) | 40, 259 |
| BciT130I | CCWGG | 1 cut(s) | 284 |
| BfmI | CTRYAG | 1 cut(s) | 57 |
| BisI | GCNGC | 1 cut(s) | 11 |
| BlsI | GCNGC | 1 cut(s) | 12 |
| Bme1390I | CCNGG | 1 cut(s) | 284 |
| BmrFI | CCNGG | 1 cut(s) | 284 |
| BsaJI | CCNNGG | 1 cut(s) | 282 |
| Bse1I | ACTGG | 1 cut(s) | 306 |
| BseBI | CCWGG | 1 cut(s) | 284 |
| BseDI | CCNNGG | 1 cut(s) | 282 |
| BseGI | GGATG | 2 cut(s) | 69, 259 |
| BseNI | ACTGG | 1 cut(s) | 306 |
| BseXI | GCAGC | 1 cut(s) | 22 |
| BsgI | GTGCAG | 1 cut(s) | 29 |
| Bsp143I | GATC | 1 cut(s) | 102 |
| BspACI | CCGC | 1 cut(s) | 41 |
| BsrI | ACTGG | 1 cut(s) | 306 |
| BssECI | CCNNGG | 1 cut(s) | 282 |
| BssMI | GATC | 1 cut(s) | 102 |
| Bst2UI | CCWGG | 1 cut(s) | 284 |
| Bst4CI | ACNGT | 1 cut(s) | 247 |
| Bst6I | CTCTTC | 1 cut(s) | 284 |
| BstF5I | GGATG | 2 cut(s) | 69, 259 |
| BstKTI | GATC | 1 cut(s) | 105 |
| BstMBI | GATC | 1 cut(s) | 102 |
| BstNI | CCWGG | 1 cut(s) | 284 |
| BstSCI | CCNGG | 1 cut(s) | 282 |
| BstSFI | CTRYAG | 1 cut(s) | 57 |
| BstV1I | GCAGC | 1 cut(s) | 22 |
| BtsCI | GGATG | 2 cut(s) | 69, 259 |
| CviAII | CATG | 1 cut(s) | 92 |
| CviJI | RGCY | 2 cut(s) | 13, 318 |
| CviKI_1 | RGCY | 2 cut(s) | 13, 318 |
| DpnI | GATC | 1 cut(s) | 104 |
| DpnII | GATC | 1 cut(s) | 102 |
| Eam1104I | CTCTTC | 1 cut(s) | 284 |
| EarI | CTCTTC | 1 cut(s) | 284 |
| EcoRII | CCWGG | 1 cut(s) | 282 |
| FaeI | CATG | 1 cut(s) | 95 |
| FaiI | YATR | 8 cut(s) | 21, 81, 83, 93, 107, 227, 241, 243 |
| FatI | CATG | 1 cut(s) | 91 |
| Fnu4HI | GCNGC | 1 cut(s) | 11 |
| FokI | GGATG | 2 cut(s) | 76, 266 |
| Fsp4HI | GCNGC | 1 cut(s) | 11 |
| GluI | GCNGC | 1 cut(s) | 11 |
| Hin1II | CATG | 1 cut(s) | 95 |
| HincII | GTYRAC | 1 cut(s) | 73 |
| HindII | GTYRAC | 1 cut(s) | 73 |
| HinfI | GANTC | 2 cut(s) | 88, 236 |
| HphI | GGTGA | 3 cut(s) | 55, 97, 298 |
| Hpy166II | GTNNAC | 1 cut(s) | 73 |
| Hpy188III | TCNNGA | 2 cut(s) | 25, 171 |
| Hpy8I | GTNNAC | 1 cut(s) | 73 |
| Hpy99I | CGWCG | 1 cut(s) | 19 |
| HpyAV | CCTTC | 1 cut(s) | 290 |
| HpyCH4III | ACNGT | 1 cut(s) | 247 |
| HpyCH4V | TGCA | 1 cut(s) | 10 |
| Hsp92II | CATG | 1 cut(s) | 95 |
| Kzo9I | GATC | 1 cut(s) | 102 |
| LpnPI | CCDG | 4 cut(s) | 45, 269, 287, 296 |
| Lsp1109I | GCAGC | 1 cut(s) | 22 |
| MalI | GATC | 1 cut(s) | 104 |
| MboI | GATC | 1 cut(s) | 102 |
| MboII | GAAGA | 4 cut(s) | 190, 227, 274, 301 |
| MluCI | AATT | 4 cut(s) | 130, 182, 218, 274 |
| MlyI | GAGTC | 1 cut(s) | 97 |
| MnlI | CCTC | 4 cut(s) | 89, 114, 167, 308 |
| MspR9I | CCNGG | 1 cut(s) | 284 |
| MvaI | CCWGG | 1 cut(s) | 284 |
| NdeII | GATC | 1 cut(s) | 102 |
| NlaIII | CATG | 1 cut(s) | 95 |
| PfeI | GAWTC | 1 cut(s) | 236 |
| PkrI | GCNGC | 1 cut(s) | 12 |
| PleI | GAGTC | 1 cut(s) | 96 |
| PpsI | GAGTC | 1 cut(s) | 96 |
| Psp6I | CCWGG | 1 cut(s) | 282 |
| PspGI | CCWGG | 1 cut(s) | 282 |
| SatI | GCNGC | 1 cut(s) | 11 |
| Sau3AI | GATC | 1 cut(s) | 102 |
| SchI | GAGTC | 1 cut(s) | 97 |
| ScrFI | CCNGG | 1 cut(s) | 284 |
| SetI | ASST | 5 cut(s) | 9, 100, 125, 205, 288 |
| SfcI | CTRYAG | 1 cut(s) | 57 |
| Sse9I | AATT | 4 cut(s) | 130, 182, 218, 274 |
| SsiI | CCGC | 1 cut(s) | 41 |
| StyD4I | CCNGG | 1 cut(s) | 282 |
| TaaI | ACNGT | 1 cut(s) | 247 |
| TaqI | TCGA | 2 cut(s) | 170, 313 |
| TasI | AATT | 4 cut(s) | 130, 182, 218, 274 |
| TfiI | GAWTC | 1 cut(s) | 236 |
| TseI | GCWGC | 1 cut(s) | 10 |
| XapI | RAATTY | 2 cut(s) | 182, 274 |