Rh6AG299700

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0005522
Physical Location & Seq
Forward (+)
49904874 .. 49906913
2040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG299700.1

Sequence Viewer

Length: 2040 bp
ATGCCATCCACCCTTAAACCCTTCGGGAACCCACAATCAGTCTCAAAAATCATGAAGAAGCCAGAAGTTAGCGACTCTGATGATGAGGAATATGACTTCTCAGAACCTTGCCACTCTAGTGAAGAAGAATATAACTCCTCAGAATCTTGTGACGACTCTTCTGATGATGATGAATCAGACTCCTCAGAAGAAAACTCGATCGAAACTGAAACGGCTGCGTCCACAGTGACGATCTTGGTGAAGTTTTCGTCAAGCAAAGAGGAGAGGAACTTGTTCAAACTAAAGAGTTCAGATACCATCTCCCGCCTCAAGATCATGATCAAAACCGAGCATGGCGTCACAAGAAAGCGCCAAACCCTAGTTTACAGAGGCAGAGTGCTTGAAAACCACGAAACCCTAGCAGCATGCAAGGTCGAAAGGTGGTCCATTATCCATGCTATTTTCTGTCCAGAAGAAATTAGCAATCCCAATGTGGGTGGGGTAGACATGGCTGTGAAAGTAGTGTCTGATGCCTTGAGGGTGGAAGTGAAACCCTGGTATACCGGCCAAGAAATAAAGGCGATCATGGAGAGCATGGCGGGGATTTCCTTGGGCGATCATGTCCTCAGCCATTGGGGGAAGCTCATAGAGGATTCGAGTCGCGTATTAAGTACTGAAGGCAGCAGCAACATGATCAATAATGAGGACGAGCCCCCCATTTATTTACTGTCAAAGTTTCAGATTACCGCACGAGGCTGTGGTTGGGAAGAGGTGATTATGGTGAGTGAATCACATACTGTCCTAGATGTAAAGAAGATGGTTGAAGATTTTGCCGAGGTGAAACCTGAAAACCTGATGCTTTGTCATGGCAATGGTGGCGATGATAAGATGGTTTGTCTCGATGATGATGAGAAAACTCTTCTGTTTTATGATGTAAAAGAAAAAGCTACGATTTGGGTACTTGGCAGATCTTGGAAATTGACCATCAAGATGCCACAGGTGGGAGATAAATTCGTGCTTAGCTACGATAAGGTCGGTACCATAAAGGACATTAAGGATTATATATGTAAGAAGATGGGGATTCCATGTAGCAGTCAGAAGCTTTTTCGTGAAGGGCAGTTGCTTGATGATGAAGCAGCATCCCTAGAAACATTCGACAACCAGCTGGAAGCTGAGAATAGTGTTATTTATGTGACTTTCGGGGCTGGCGATGATGACCAAGTCGACGAAATAGAAGATATAAGCTTAATATTTGAGAGAGACAAAAATGAAACGGGTTTGATTGAATACAAGGTGGTTTATAAGAGCGAATCAGATGAGAACAGTGAATCAGGTGAAAGCAGCGATTCTGATAGTGATTCATCAGATTTAGTCTCTTCTTTCGAAATCAAGTTGAAATCATCTTGCACGATTTTCGATGTCAAGGCAGCAATCGAGAGCCTAAACAGCTTTCGAATTTGTACCCAAAAGCTATACTGGGATGATCAAATTCTGGAAGATTGGGAAACAGTGGACGACGACATTACAAAGTGGTCCAGTGTCCCTGCTCCATTAACAATACACTTTGAGACACCCAGTTCTAGTTCTAATGTGGGGGCCAATGAAAAGATTGTTTCTGCAGTTGACCGTGCAGACGAAAGCCTTGACGACGTTTTGGTTCCACAAAAGGTGCTGGAAAGAATCCATGGGCAGAGAGGTTTCTTCCTCGATGGTATTTTACTCGAGGCATGCACATTGCTTTCTCAGATTGAAGCTGGTTCTGTCTTATGCTTAAACAGTGTGGTGCAGATAAATGTTACTGGGCCGAATGGTGCAACGACCAAGGTGCATGTGAACCCTTCTTACAAATTCAAGGATGTAAAAGAGAAGATTGAACTAGATTGTGGAATCCCATTTGGTTTACAGAGACTTACTCATGATGGTGATGAACTTAATGATGATATGACTATAGGGCTTTATGAGGCATCGTTGGGCAGTCTGTGGAAATTAAGGTGGAACTTTTGTGGTACTTCCTGGTCATTCTTCTACCCGTGGTACAAGGATTGTTGCAGGACTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
Motile_Sperm Pkinase ubiquitin

Protein Analysis

679

Amino Acids

76.09

Weight (kDa)

4.54

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 79 - 146 9.8e-12 Ubiquitin family
Rad60-SLD PF11976 589 - 647 2.5e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 590 - 647 6.6e-06 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1281
Acc65I GGTACC 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 1016
AccI GTMKAC 3 cut(s) 483, 539, 1203
AccII CGCG 1 cut(s) 642
AciI CCGC 3 cut(s) 304, 578, 726
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 4 cut(s) 989, 1434, 1467, 1826
AcuI CTGAAG 1 cut(s) 675
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 6 cut(s) 652, 939, 1018, 1441, 1987, 2015
AfiI CCNNNNNNNGG 2 cut(s) 473, 980
AgsI TTSAA 8 cut(s) 277, 383, 803, 1265, 1375, 1730, 1831, 1853
AjnI CCWGG 2 cut(s) 533, 1991
AleI CACNNNNGTG 1 cut(s) 117
Alw26I GTCTC 6 cut(s) 46, 881, 1233, 1357, 1541, 1879
Ama87I CYCGRG 1 cut(s) 1700
AoxI GGCC 3 cut(s) 544, 1575, 1781
ApeKI GCWGC 7 cut(s) 215, 401, 660, 663, 1115, 1320, 1406
ApoI RAATTY 4 cut(s) 989, 1434, 1467, 1826
Asp700I GAANNNNTTC 1 cut(s) 272
Asp718I GGTACC 1 cut(s) 1016
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 4 cut(s) 423, 1512, 1575, 1781
AsuHPI GGTGA 6 cut(s) 250, 763, 772, 829, 1325, 1913
AsuII TTCGAA 2 cut(s) 1362, 1432
AvaI CYCGRG 1 cut(s) 1700
AvaII GGWCC 2 cut(s) 423, 1512
BanI GGYRCC 1 cut(s) 1016
BanII GRGCYC 1 cut(s) 693
BauI CACGAG 1 cut(s) 729
BbvCI CCTCAGC 1 cut(s) 605
BbvI GCAGC 7 cut(s) 202, 413, 672, 675, 1127, 1332, 1418
BccI CCATC 8 cut(s) 13, 305, 790, 862, 971, 1048, 1682, 1892
BceAI ACGGC 1 cut(s) 228
BcgI CGANNNNNNTGC 4 cut(s) 1375, 1409, 1786, 1820
BciT130I CCWGG 2 cut(s) 535, 1993
BclI TGATCA 3 cut(s) 318, 672, 1462
BcoDI GTCTC 6 cut(s) 46, 881, 1233, 1357, 1541, 1879
BfaI CTAG 8 cut(s) 117, 359, 398, 782, 1124, 1560, 1856, 2038
BfmI CTRYAG 2 cut(s) 1596, 1926
BfoI RGCGCY 1 cut(s) 352
BglII AGATCT 1 cut(s) 947
BisI GCNGC 7 cut(s) 216, 402, 661, 664, 1116, 1321, 1407
BlpI GCTNAGC 1 cut(s) 998
BlsI GCNGC 7 cut(s) 217, 403, 662, 665, 1117, 1322, 1408
BmcAI AGTACT 1 cut(s) 652
Bme1390I CCNGG 2 cut(s) 535, 1993
Bme18I GGWCC 2 cut(s) 423, 1512
BmeT110I CYCGRG 1 cut(s) 1700
BmgT120I GGNCC 4 cut(s) 423, 1512, 1575, 1781
BmiI GGNNCC 4 cut(s) 29, 1018, 1576, 1638
BmrFI CCNGG 2 cut(s) 535, 1993
BmrI ACTGGG 3 cut(s) 1465, 1548, 1788
BmsI GCATC 5 cut(s) 499, 825, 960, 1127, 1952
BmuI ACTGGG 3 cut(s) 1465, 1548, 1788
BplI GAGNNNNNCTC 2 cut(s) 1876, 1908
Bpu10I CCTNAGC 1 cut(s) 605
Bpu1102I GCTNAGC 1 cut(s) 998
Bpu14I TTCGAA 2 cut(s) 1362, 1432
BpuEI CTTGAG 2 cut(s) 293, 535
BsaBI GATNNNNATC 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 6 cut(s) 533, 588, 813, 1663, 1800, 2009
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 980
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 5 cut(s) 1460, 1515, 1554, 1783, 2038
Bse3DI GCAATG 2 cut(s) 856, 1712
Bse8I GATNNNNATC 1 cut(s) 317
BseBI CCWGG 2 cut(s) 535, 1993
BseDI CCNNGG 6 cut(s) 533, 588, 813, 1663, 1800, 2009
BseGI GGATG 4 cut(s) 5, 1118, 1465, 1840
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 2 cut(s) 473, 980
BseMI GCAATG 2 cut(s) 856, 1712
BseMII CTCAG 6 cut(s) 114, 153, 198, 619, 1143, 1736
BseNI ACTGG 5 cut(s) 1460, 1515, 1554, 1783, 2038
BseRI GAGGAG 3 cut(s) 127, 172, 275
BseXI GCAGC 7 cut(s) 202, 413, 672, 675, 1127, 1332, 1418
BsgI GTGCAG 2 cut(s) 1629, 1784
Bsh1236I CGCG 1 cut(s) 642
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 3 cut(s) 546, 1577, 1783
BshNI GGYRCC 1 cut(s) 1016
BsiEI CGRYCG 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 1700
BsiSI CCGG 1 cut(s) 543
BslFI GGGAC 1 cut(s) 1505
BslI CCNNNNNNNGG 2 cut(s) 473, 980
BsmAI GTCTC 6 cut(s) 46, 881, 1233, 1357, 1541, 1879
BsmFI GGGAC 1 cut(s) 1505
BsnI GGCC 3 cut(s) 546, 1577, 1783
BsoBI CYCGRG 1 cut(s) 1700
Bsp119I TTCGAA 2 cut(s) 1362, 1432
Bsp1286I GDGCHC 1 cut(s) 693
Bsp143I GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
Bsp1720I GCTNAGC 1 cut(s) 998
Bsp19I CCATGG 1 cut(s) 1663
BspACI CCGC 3 cut(s) 304, 578, 726
BspANI GGCC 3 cut(s) 546, 1577, 1783
BspCNI CTCAG 6 cut(s) 113, 152, 197, 618, 1144, 1735
BspFNI CGCG 1 cut(s) 642
BspHI TCATGA 3 cut(s) 51, 315, 1894
BspLI GGNNCC 4 cut(s) 29, 1018, 1576, 1638
BspMAI CTGCAG 1 cut(s) 1600
BspT104I TTCGAA 2 cut(s) 1362, 1432
BspT107I GGYRCC 1 cut(s) 1016
BsrDI GCAATG 2 cut(s) 856, 1712
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 5 cut(s) 1460, 1515, 1554, 1783, 2038
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 6 cut(s) 533, 588, 813, 1663, 1800, 2009
BssMI GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
BssNAI GTATAC 1 cut(s) 540
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 729
BssT1I CCWWGG 3 cut(s) 588, 1663, 1800
Bst1107I GTATAC 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 729
Bst2UI CCWGG 2 cut(s) 535, 1993
Bst4CI ACNGT 7 cut(s) 226, 708, 778, 1304, 1489, 1607, 1757
Bst6I CTCTTC 4 cut(s) 163, 741, 903, 1360
BstACI GRCGYC 1 cut(s) 336
BstBI TTCGAA 2 cut(s) 1362, 1432
BstC8I GCNNGC 3 cut(s) 406, 1186, 1708
BstDEI CTNAG 7 cut(s) 100, 139, 184, 605, 998, 1152, 1722
BstDSI CCRYGG 2 cut(s) 1663, 2009
BstF5I GGATG 4 cut(s) 5, 1118, 1465, 1840
BstFNI CGCG 1 cut(s) 642
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 9 cut(s) 201, 234, 315, 321, 564, 598, 675, 950, 1465
BstMAI GTCTC 6 cut(s) 46, 881, 1233, 1357, 1541, 1879
BstMBI GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 3 cut(s) 855, 1425, 2034
BstNI CCWGG 2 cut(s) 535, 1993
BstNSI RCATGY 3 cut(s) 408, 1710, 1811
BstSCI CCNGG 2 cut(s) 533, 1991
BstSFI CTRYAG 2 cut(s) 1596, 1926
BstUI CGCG 1 cut(s) 642
BstV1I GCAGC 7 cut(s) 202, 413, 672, 675, 1127, 1332, 1418
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
BstZ17I GTATAC 1 cut(s) 540
BsuRI GGCC 3 cut(s) 546, 1577, 1783
BtgI CCRYGG 2 cut(s) 1663, 2009
BtgZI GCGATG 2 cut(s) 873, 1203
BtsCI GGATG 4 cut(s) 5, 1118, 1465, 1840
BtsIMutI CAGTG 5 cut(s) 231, 1309, 1494, 1522, 1762
Cac8I GCNNGC 3 cut(s) 406, 1186, 1708
CciI TCATGA 3 cut(s) 51, 315, 1894
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 4 cut(s) 423, 1512, 1575, 1781
CseI GACGC 2 cut(s) 207, 325
Csp6I GTAC 6 cut(s) 651, 938, 1017, 1440, 1986, 2014
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviQI GTAC 6 cut(s) 651, 938, 1017, 1440, 1986, 2014
DdeI CTNAG 7 cut(s) 100, 139, 184, 605, 998, 1152, 1722
DpnI GATC 9 cut(s) 200, 233, 314, 320, 563, 597, 674, 949, 1464
DpnII GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 4 cut(s) 163, 741, 903, 1360
EarI CTCTTC 4 cut(s) 163, 741, 903, 1360
Eco130I CCWWGG 3 cut(s) 588, 1663, 1800
Eco24I GRGCYC 1 cut(s) 693
Eco47I GGWCC 2 cut(s) 423, 1512
Eco57I CTGAAG 1 cut(s) 675
Eco88I CYCGRG 1 cut(s) 1700
EcoRII CCWGG 2 cut(s) 533, 1991
EcoT14I CCWWGG 3 cut(s) 588, 1663, 1800
EcoT38I GRGCYC 1 cut(s) 693
ErhI CCWWGG 3 cut(s) 588, 1663, 1800
FaqI GGGAC 1 cut(s) 1505
FauI CCCGC 2 cut(s) 311, 571
FbaI TGATCA 3 cut(s) 318, 672, 1462
FblI GTMKAC 3 cut(s) 483, 539, 1203
Fnu4HI GCNGC 7 cut(s) 216, 402, 661, 664, 1116, 1321, 1407
FokI GGATG 3 cut(s) 1105, 1472, 1847
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 7 cut(s) 216, 402, 661, 664, 1116, 1321, 1407
FspBI CTAG 8 cut(s) 117, 359, 398, 782, 1124, 1560, 1856, 2038
GlaI GCGC 1 cut(s) 350
GluI GCNGC 7 cut(s) 216, 402, 661, 664, 1116, 1321, 1407
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 546, 1577, 1783
HapII CCGG 1 cut(s) 543
HgaI GACGC 2 cut(s) 207, 325
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 336
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HincII GTYRAC 2 cut(s) 1204, 1603
HindII GTYRAC 2 cut(s) 1204, 1603
HindIII AAGCTT 2 cut(s) 1079, 1222
HpaII CCGG 1 cut(s) 543
HphI GGTGA 6 cut(s) 250, 763, 772, 829, 1325, 1913
Hpy166II GTNNAC 9 cut(s) 222, 364, 484, 540, 1204, 1492, 1603, 1813, 1880
Hpy8I GTNNAC 9 cut(s) 222, 364, 484, 540, 1204, 1492, 1603, 1813, 1880
Hpy99I CGWCG 3 cut(s) 1208, 1499, 1631
HpyAV CCTTC 4 cut(s) 31, 650, 1085, 1827
HpyCH4III ACNGT 7 cut(s) 226, 708, 778, 1304, 1489, 1607, 1757
HpyCH4IV ACGT 1 cut(s) 1629
HpyCH4V TGCA 9 cut(s) 408, 1386, 1598, 1610, 1710, 1765, 1793, 1807, 2028
HpyF10VI GCNNNNNNNGC 3 cut(s) 855, 1425, 2034
HpyF3I CTNAG 7 cut(s) 100, 139, 184, 605, 998, 1152, 1722
HpySE526I ACGT 1 cut(s) 1629
Hsp92I GRCGYC 1 cut(s) 336
HspAI GCGC 1 cut(s) 349
KpnI GGTACC 1 cut(s) 1020
Ksp22I TGATCA 3 cut(s) 318, 672, 1462
Kzo9I GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
LmnI GCTCC 1 cut(s) 1531
Lsp1109I GCAGC 7 cut(s) 202, 413, 672, 675, 1127, 1332, 1418
LweI GCATC 5 cut(s) 499, 825, 960, 1127, 1952
MaeI CTAG 8 cut(s) 117, 359, 398, 782, 1124, 1560, 1856, 2038
MaeII ACGT 1 cut(s) 1629
MaeIII GTNAC 5 cut(s) 149, 226, 337, 1171, 1774
MalI GATC 9 cut(s) 200, 233, 314, 320, 563, 597, 674, 949, 1464
MboI GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 693
MluCI AATT 7 cut(s) 456, 956, 989, 1434, 1467, 1826, 1964
MlyI GAGTC 4 cut(s) 68, 149, 173, 646
MroXI GAANNNNTTC 1 cut(s) 272
MseI TTAA 8 cut(s) 15, 647, 1032, 1226, 1532, 1751, 1911, 1967
MslI CAYNNNNRTG 5 cut(s) 117, 491, 849, 968, 1899
MspA1I CMGCKG 1 cut(s) 1144
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 2 cut(s) 535, 1993
MvaI CCWGG 2 cut(s) 535, 1993
MvnI CGCG 1 cut(s) 642
MwoI GCNNNNNNNGC 3 cut(s) 855, 1425, 2034
NcoI CCATGG 1 cut(s) 1663
NdeII GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
NlaIV GGNNCC 4 cut(s) 29, 1018, 1576, 1638
NmeAIII GCCGAG 1 cut(s) 838
NmuCI GTSAC 4 cut(s) 149, 226, 337, 1171
NspI RCATGY 3 cut(s) 408, 1710, 1811
NspV TTCGAA 2 cut(s) 1362, 1432
OliI CACNNNNGTG 1 cut(s) 117
PaeI GCATGC 2 cut(s) 408, 1710
PaeR7I CTCGAG 1 cut(s) 1700
PagI TCATGA 3 cut(s) 51, 315, 1894
PcsI WCGNNNNNNNCGW 1 cut(s) 1185
PdmI GAANNNNTTC 1 cut(s) 272
PflFI GACNNNGTC 1 cut(s) 1199
PkrI GCNGC 7 cut(s) 217, 403, 662, 665, 1117, 1322, 1408
Ple19I CGATCG 1 cut(s) 201
PleI GAGTC 4 cut(s) 68, 149, 173, 645
PpsI GAGTC 4 cut(s) 68, 149, 173, 645
PsiI TTATAA 1 cut(s) 1281
Psp6I CCWGG 2 cut(s) 533, 1991
PspGI CCWGG 2 cut(s) 533, 1991
PspN4I GGNNCC 4 cut(s) 29, 1018, 1576, 1638
PspPI GGNCC 4 cut(s) 423, 1512, 1575, 1781
PspXI VCTCGAGB 1 cut(s) 1700
PsrI GAACNNNNNNTAC 2 cut(s) 1805, 1837
PstI CTGCAG 1 cut(s) 1600
PsuI RGATCY 1 cut(s) 947
PsyI GACNNNGTC 1 cut(s) 1199
PvuI CGATCG 1 cut(s) 201
PvuII CAGCTG 1 cut(s) 1144
RsaI GTAC 6 cut(s) 652, 939, 1018, 1441, 1987, 2015
RsaNI GTAC 6 cut(s) 651, 938, 1017, 1440, 1986, 2014
RseI CAYNNNNRTG 5 cut(s) 117, 491, 849, 968, 1899
SalI GTCGAC 1 cut(s) 1202
SaqAI TTAA 8 cut(s) 15, 647, 1032, 1226, 1532, 1751, 1911, 1967
SatI GCNGC 7 cut(s) 216, 402, 661, 664, 1116, 1321, 1407
Sau3AI GATC 9 cut(s) 198, 231, 312, 318, 561, 595, 672, 947, 1462
Sau96I GGNCC 4 cut(s) 423, 1512, 1575, 1781
ScaI AGTACT 1 cut(s) 652
SchI GAGTC 4 cut(s) 68, 149, 173, 646
ScrFI CCNGG 2 cut(s) 535, 1993
SduI GDGCHC 1 cut(s) 693
SfaNI GCATC 5 cut(s) 499, 825, 960, 1127, 1952
SfcI CTRYAG 2 cut(s) 1596, 1926
Sfr274I CTCGAG 1 cut(s) 1700
SfuI TTCGAA 2 cut(s) 1362, 1432
SinI GGWCC 2 cut(s) 423, 1512
SlaI CTCGAG 1 cut(s) 1700
SmiMI CAYNNNNRTG 5 cut(s) 117, 491, 849, 968, 1899
SmlI CTYRAG 3 cut(s) 308, 514, 1700
SmoI CTYRAG 3 cut(s) 308, 514, 1700
SphI GCATGC 2 cut(s) 408, 1710
Sse9I AATT 7 cut(s) 456, 956, 989, 1434, 1467, 1826, 1964
SsiI CCGC 3 cut(s) 304, 578, 726
SspI AATATT 1 cut(s) 1230
SspMI CTAG 8 cut(s) 117, 359, 398, 782, 1124, 1560, 1856, 2038
StyD4I CCNGG 2 cut(s) 533, 1991
StyI CCWWGG 3 cut(s) 588, 1663, 1800
TaaI ACNGT 7 cut(s) 226, 708, 778, 1304, 1489, 1607, 1757
TaiI ACGT 1 cut(s) 1632
TasI AATT 7 cut(s) 456, 956, 989, 1434, 1467, 1826, 1964
TatI WGTACW 1 cut(s) 650
Tru1I TTAA 8 cut(s) 15, 647, 1032, 1226, 1532, 1751, 1911, 1967
Tru9I TTAA 8 cut(s) 15, 647, 1032, 1226, 1532, 1751, 1911, 1967
TscAI CASTG 5 cut(s) 231, 1309, 1494, 1522, 1762
TseFI GTSAC 4 cut(s) 149, 226, 337, 1171
TseI GCWGC 7 cut(s) 215, 401, 660, 663, 1115, 1320, 1406
Tsp45I GTSAC 4 cut(s) 149, 226, 337, 1171
TspDTI ATGAA 7 cut(s) 68, 186, 1125, 1263, 1329, 1596, 1920
TspRI CASTG 5 cut(s) 231, 1309, 1494, 1522, 1762
Tth111I GACNNNGTC 1 cut(s) 1199
VpaK11BI GGWCC 2 cut(s) 423, 1512
XapI RAATTY 4 cut(s) 989, 1434, 1467, 1826
XceI RCATGY 3 cut(s) 408, 1710, 1811
XhoI CTCGAG 1 cut(s) 1700
XmiI GTMKAC 3 cut(s) 483, 539, 1203
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 8 cut(s) 117, 359, 398, 782, 1124, 1560, 1856, 2038
ZrmI AGTACT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.