Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Forward (+)

22400264 .. 22407516

Gene Structure 7253 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g28650.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGGCGTCATCATCGTTAACACTGAGTAAAGACCTGGCCTTTTTGATCATGCAATTCCTTGACGAGGAAAAGTGGAAAGAAACGCTTCATAAGCTTCAGCAAGAATCAGGATACCACTTCGACTTGAAGTATTTCGAGGAATTATTACTGGAAGGAAATTGGATTGAAGTCGAGAAGTATCTTTCCGGTTTCACTAAACTGGATGACAACCGCTATTCCATGAAGATATTCTTCGAAATTCGTAAGCAGAAGTATTTAGAGGCCCTAGACAGACAAGATAGAGCTGCCGCAGTAGATATCCTAGTGAAGGATTTGAAAGTCTTTGCGGGGTTTAATGATGAGTTGTACAAGGAAATCACTCAGCTGCTCACCTTGAACAATTTTAGAGACAATGCTCAGCTCTCTGCTTATGCAGATGTAAGGACTGCAAGAGCAATTATGGCCCAAGAACTTAAGAAGCTTGTTGAGGCCAACCCTCTTTTCTGTGACAAATTAACGTTTCCCAATTTGAGAAGTTCAAGGCTACGGGCGCTCATTAACCAAAGCTTAAATTGGCAGCATTGCCTTTGTCTAAATCCTAAACAAAATCCGGAAATAAAAACCTTATTTGTGGATCACACTTGTCATAGCAGACATTCCAACGATCCATTTGCTCACATAGCAGCAAATGGTCAGGCTCCGCAGAGAGGTGCAGTACAGAGAGCTGATGGCTTCAATTCTGTCGGTGTCAATGGGTCCTTTCAGCCCTCCACTGCACCTATACCAATTCAATCTCCTTTGTCAACATGGACGCCTATCCCGCCCAGTGTGAATCAGTTAGCGGCTTCAGCAGGAGGTATTGGTTTTGGAATTCAAGCAAATCCAGCTTCATTTTCGACTGGTCCAGCAAATCCTGAAGATTTATTCAGAACGCGGTTCCCTGGGGTTTCAGACAGGGTTACAACGCCAGGGATTAATCCAGGCCATGTTCTAAGTCCAACATTCAATGCAGTAGATGACTTCCCCAAGGCTGTAGCTCGGATACTGAATCAGGGCTCAGTTGCAACTAGTGTAGACTTTCATCCTCTCCAACAAACTATACTTCTTGTTGGAACTAATGTGGGGGATATAAGTATCTGGGAAGTCAGTTCTAGAGAGAAGTTGGTATCAAGAAGCTTTCAGCCATGGGATATGGGGGCAAGTTCAATGGCATTAAAGGCAACTTTAGTCAAGGATCCAAGTGTCTCAGTTAAGCGTGTACTATGGAGCCCTGATGGTTCTCTATTTGGAGTTGCATACTCTAAACACATGATGCAGTTATATGGATATCTTGGTGGCGATGATGTTCATCAACATAAAGAGATCGAAGCTCATGTTGGTAGTGTAAATGATCTGGCGTTCTGCAACCTAAGAAAGCAACTCTGTGTCATAACCTGCGGTGATGACAAGGCCATCAAGGTCTGGGATGCGGTTACTGGATCAAAGTTGTATACTTTTGAAGGTCATGAGGCTCCTGTTCATTCTGTGTGCCCTCATAGCAAAGAAGATATCCATTTTGTCTTTTCGACATCAGTAGATGGACAAATAAAAGCATGGTTATACGAAGTAGATGGGCCAAGGGTTAATTACGATGCTCCCAGCCTCTCATGCACAACAATGGTTTATAGTGCAGATGGTAAAAGGCTGTTTTCATGTGGGACAAGCAAAGATGGAGAATCTCATGTTGTTGAATGGAATGAAAATGAAGGCACAATAAAGAGGAATTACCAAGGATTTCAAAAACAGTCTTTTGGTATTGTTCAATTTGACACAACCAGAAATAAGTATTTGGCAGTTGGTGACAACCATGCAATAAAAGTTTGGGATATGGATAATGTCAATCTCTTGTTTACTGTTGATTGTGAAGGAGGTTTACCAGCAACCCCTCATATCCGCTTCAACAAGGAAGGATCTTTGATGGCGGTGTCTACAAATGACAACAGAATCAAAGTTTTGGCTACAACAGATGGTGTTCAGTTGATGCGCAATTATGAAAGTCTCTCGCTTATTGCCTCAAGAAATGCACCAGAAAAGAATGGTAGTACCAGAAACATGGGGGATAATAAGAAAGCAAGGTTAAGTGAAGAGCTCAACACAAAAAAGATCTGGAAGCTCACTGAGATTAGTGAATCTGCTCAGCTTCGATCTTTAAGGCTATCAACGACAACTGTTAAAACAGACAAGATCTCAAGGTTGGTGTACACTAATTCAGGTAATGCCATTCTGGCATTAGCATCAAATGGAACCCATCTACTGTGGAGGTGGCGACGAGTTGACGGTAACACGAGTGGCAAGGCAACAACCAAGGTTACCCCACATTCCATGGAACCAGCTTCAGGCATCCTGATGGTCAATGATCTTACTGGTGCTAAACCAGAACATGCAATACCTTGTTGCGCTTTGTCCAAGAATGATTCGTATGTCATGTCAGCCTCCGGAGGCAAGATATCGTTGTTCAACCTAATGACATTCAAGACAATGACAAGTTTCATGTCGCCGCCACCTGTGGTAACATGCCTTGCTTTCCACCCTGAAGACAATAATATAGTTGCTGTTGGTTTTGATGATTCGACTATTCACATATACAATGTCCGCATTGACGAGGTTAGGATAAAGCTCAAGGGTCACTCTAAGACAGTCACAGGCCTCGCATTCTCTCAACTTTTGAAGACACTTATTTCTTCTGGAGCAGATTGTCAGATAGTTGTGTGGAGCTCTGATAAGTGGGAAAGGCAGAAGAACAGTTCCTTGCATATTCCAGCTTGGACAACACCTACAGCACTGTTTGGAACTCATCTTCAGTATCAGAAGGATCAGAATCACTTTCTCGTTGTACACGAGACTCAACTTGCTATATACGAAACCTTGAGGCTTGAATGCCAGAAGAAGTGGGTAGTAGGAGAATCTTCAGCACCAATCTCCTATGCAACACTCTCTTGTGATAGCCAGATAGTCTACGCGAGCTTTCTGGATGGAACGTTGCGTGTGTTTGCAGCATCAAATCTTCAAATGCAATGCCAGATTAACCCCTATGCTTATCTTCCACCTAATGTCAGCTCTGCAATATACCCCTCAGTAGTAGCTGCGAATCCACAAGAGCCGAACCAGTTTGCAGTGGGACTAACAGATGGTGCAGTTGTCATGTTTGAACCACTCGAGTCTGGAGACAAATGGGGTGTGCCTCCACCGGCAAACATTGGCTACTTCCATGCTTCAACTTCTGCCAGTGGTTCCAGTTTGGAACAACACCATAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1092

Amino Acids

120.72

Weight (kDa)

6.72

Isoelectric Point (pI)

35.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	9 - 37	5.4e-11	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	42 - 165	1.7e-57	TPR1-like, CTLH-containing domain
zf_topless	PF21359	168 - 208	1.2e-22	TOPLESS, zinc finger domain
WDR55	PF24796	346 - 525	9.2e-06	WDR55
Beta-prop_WDR3_1st	PF25173	389 - 560	1.9e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	404 - 559	3.8e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	409 - 619	3.4e-18	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	409 - 667	3.2e-22	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	416 - 525	2.4e-09	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	445 - 659	4.2e-15	Prp19 WD40 domain
Beta-prop_EML	PF23409	450 - 655	5.4e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd	PF25047	460 - 526	6e-06	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	465 - 528	4.6e-09	WDHD1 first WD40 domain
EIF3I	PF24805	469 - 673	1.1e-07	EIF3I
WDR55	PF24796	496 - 658	6.5e-08	WDR55
Beta-prop_WDR5	PF25175	512 - 665	7.6e-07	WDR5 beta-propeller domain
WD40_Prp19	PF24814	797 - 994	8.4e-13	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	798 - 922	1.7e-12	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	799 - 940	1.1e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	799 - 1007	5e-18	WDR5 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	801 - 1037	2.1e-06	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	802 - 1007	4.2e-17	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	804 - 1010	3e-11	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	808 - 911	3.2e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	813 - 911	1.9e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	815 - 911	2.2e-10	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	815 - 923	4.9e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	820 - 914	4.7e-09	WDR90/POC16, second beta-propeller
Beta-prop_THOC3	PF25174	843 - 1010	1.2e-07	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	855 - 910	3.2e-06	MABP1/WDR62 second WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2003
Acc36I	ACCTGC	1 cut(s)	1421
AccI	GTMKAC	4 cut(s)	1053, 1469, 1946, 2973
AccII	CGCG	2 cut(s)	913, 2978
AccIII	TCCGGA	2 cut(s)	589, 2453
AclI	AACGTT	2 cut(s)	497, 2996
AclWI	GGATC	7 cut(s)	621, 638, 1208, 1221, 1465, 1936, 2838
AcsI	RAATTY	2 cut(s)	237, 849
AcuI	CTGAAG	7 cut(s)	80, 810, 915, 2337, 2571, 2801, 2910
AcyI	GRCGYC	2 cut(s)	5, 791
AfaI	GTAC	6 cut(s)	347, 696, 1239, 2062, 2219, 2853
AfiI	CCNNNNNNNGG	4 cut(s)	64, 307, 686, 2354
AflII	CTTAAG	1 cut(s)	452
AhlI	ACTAGT	1 cut(s)	1046
AjnI	CCWGG	4 cut(s)	33, 919, 946, 958
AjuI	GAANNNNNNNTTGG	4 cut(s)	464, 496, 1338, 1370
Alw21I	GWGCWC	2 cut(s)	2109, 2735
Alw26I	GTCTC	5 cut(s)	381, 1228, 2021, 2852, 3177
AlwI	GGATC	7 cut(s)	621, 638, 1208, 1221, 1465, 1936, 2838
Ama87I	CYCGRG	1 cut(s)	3173
Aor13HI	TCCGGA	2 cut(s)	589, 2453
AoxI	GGCC	8 cut(s)	36, 261, 441, 468, 961, 1428, 1592, 2662
ApeKI	GCWGC	6 cut(s)	284, 364, 556, 662, 3011, 3101
ApoI	RAATTY	2 cut(s)	237, 849
AseI	ATTAAT	1 cut(s)	954
Asp700I	GAANNNNTTC	3 cut(s)	84, 131, 227
AspLEI	GCGC	3 cut(s)	532, 2004, 2417
AspS9I	GGNCC	5 cut(s)	262, 442, 735, 881, 1592
AsuHPI	GGTGA	3 cut(s)	361, 1430, 1829
AsuII	TTCGAA	1 cut(s)	234
AvaI	CYCGRG	1 cut(s)	3173
AvaII	GGWCC	2 cut(s)	735, 881
BaeGI	GKGCMC	1 cut(s)	1511
BamHI	GGATCC	1 cut(s)	1213
BanII	GRGCYC	4 cut(s)	1037, 1250, 2109, 2735
BarI	GAAGNNNNNNTAC	2 cut(s)	1875, 1907
BauI	CACGAG	2 cut(s)	2302, 2855
BbsI	GAAGAC	2 cut(s)	2559, 2693
Bbv12I	GWGCWC	2 cut(s)	2109, 2735
BbvI	GCAGC	6 cut(s)	271, 351, 568, 674, 3023, 3088
BcgI	CGANNNNNNTGC	2 cut(s)	632, 666
BciT130I	CCWGG	4 cut(s)	35, 921, 948, 960
BciVI	GTATCC	2 cut(s)	104, 1014
BclI	TGATCA	1 cut(s)	45
BcoDI	GTCTC	5 cut(s)	381, 1228, 2021, 2852, 3177
BcuI	ACTAGT	1 cut(s)	1046
BfaI	CTAG	4 cut(s)	266, 302, 1047, 1131
BfmI	CTRYAG	2 cut(s)	1011, 2793
BfoI	RGCGCY	1 cut(s)	533
BfrI	CTTAAG	1 cut(s)	452
BfuAI	ACCTGC	1 cut(s)	1421
BfuI	GTATCC	2 cut(s)	104, 1014
BglI	GCCNNNNNGGC	1 cut(s)	2243
BglII	AGATCT	2 cut(s)	2121, 2202
BisI	GCNGC	9 cut(s)	285, 288, 365, 557, 663, 822, 2516, 3012, 3102
BlpI	GCTNAGC	2 cut(s)	396, 2154
BlsI	GCNGC	9 cut(s)	286, 289, 366, 558, 664, 823, 2517, 3013, 3103
Bme1390I	CCNGG	4 cut(s)	35, 921, 948, 960
Bme18I	GGWCC	2 cut(s)	735, 881
BmeT110I	CYCGRG	1 cut(s)	3173
BmgT120I	GGNCC	5 cut(s)	262, 442, 735, 881, 1592
BmiI	GGNNCC	9 cut(s)	678, 736, 917, 1215, 1247, 1491, 2263, 2346, 3250
BmrFI	CCNGG	4 cut(s)	35, 921, 948, 960
BmrI	ACTGGG	1 cut(s)	798
BmsI	GCATC	7 cut(s)	1281, 1435, 1600, 1989, 2261, 2367, 3023
BmuI	ACTGGG	1 cut(s)	798
BpiI	GAAGAC	2 cut(s)	2559, 2693
BpmI	CTGGAG	2 cut(s)	2724, 3201
Bpu1102I	GCTNAGC	2 cut(s)	396, 2154
Bpu14I	TTCGAA	1 cut(s)	234
BpuEI	CTTGAG	4 cut(s)	2017, 2191, 2621, 2905
BsaBI	GATNNNNATC	2 cut(s)	1326, 2835
BsaHI	GRCGYC	2 cut(s)	5, 791
BsaJI	CCNNGG	9 cut(s)	919, 920, 947, 1005, 1163, 1595, 1747, 2322, 2340
BsaWI	WCCGGW	3 cut(s)	185, 589, 2453
Bsc4I	CCNNNNNNNGG	4 cut(s)	64, 307, 686, 2354
Bse118I	RCCGGY	1 cut(s)	3205
Bse1I	ACTGG	9 cut(s)	153, 204, 804, 883, 1459, 2386, 3124, 3243, 3252
Bse3DI	GCAATG	2 cut(s)	559, 3038
Bse8I	GATNNNNATC	2 cut(s)	1326, 2835
BseAI	TCCGGA	2 cut(s)	589, 2453
BseBI	CCWGG	4 cut(s)	35, 921, 948, 960
BseDI	CCNNGG	9 cut(s)	919, 920, 947, 1005, 1163, 1595, 1747, 2322, 2340
BseGI	GGATG	5 cut(s)	208, 1060, 1450, 2358, 2995
BseJI	GATNNNNATC	2 cut(s)	1326, 2835
BseLI	CCNNNNNNNGG	4 cut(s)	64, 307, 686, 2354
BseMI	GCAATG	2 cut(s)	559, 3038
BseMII	CTCAG	8 cut(s)	14, 374, 410, 1050, 1239, 2127, 2168, 3105
BseNI	ACTGG	9 cut(s)	153, 204, 804, 883, 1459, 2386, 3124, 3243, 3252
BseSI	GKGCMC	1 cut(s)	1511
BseXI	GCAGC	6 cut(s)	271, 351, 568, 674, 3023, 3088
BseYI	CCCAGC	1 cut(s)	1616
BsgI	GTGCAG	4 cut(s)	711, 738, 1668, 3171
Bsh1236I	CGCG	2 cut(s)	913, 2978
BshFI	GGCC	8 cut(s)	38, 263, 443, 470, 963, 1430, 1594, 2664
BsiHKAI	GWGCWC	2 cut(s)	2109, 2735
BsiHKCI	CYCGRG	1 cut(s)	3173
BsiSI	CCGG	4 cut(s)	186, 590, 2454, 3206
BslFI	GGGAC	2 cut(s)	1690, 3150
BslI	CCNNNNNNNGG	4 cut(s)	64, 307, 686, 2354
BsmAI	GTCTC	5 cut(s)	381, 1228, 2021, 2852, 3177
BsmFI	GGGAC	2 cut(s)	1690, 3150
BsmI	GAATGC	2 cut(s)	2669, 2900
BsnI	GGCC	8 cut(s)	38, 263, 443, 470, 963, 1430, 1594, 2664
BsoBI	CYCGRG	1 cut(s)	3173
Bsp119I	TTCGAA	1 cut(s)	234
Bsp1286I	GDGCHC	5 cut(s)	1037, 1250, 1511, 2109, 2735
Bsp13I	TCCGGA	2 cut(s)	589, 2453
Bsp1407I	TGTACA	3 cut(s)	345, 2217, 2851
Bsp1720I	GCTNAGC	2 cut(s)	396, 2154
Bsp19I	CCATGG	2 cut(s)	1163, 2340
BspANI	GGCC	8 cut(s)	38, 263, 443, 470, 963, 1430, 1594, 2664
BspCNI	CTCAG	8 cut(s)	15, 373, 409, 1049, 1238, 2128, 2167, 3104
BspEI	TCCGGA	2 cut(s)	589, 2453
BspFNI	CGCG	2 cut(s)	913, 2978
BspHI	TCATGA	1 cut(s)	1483
BspLI	GGNNCC	9 cut(s)	678, 736, 917, 1215, 1247, 1491, 2263, 2346, 3250
BspMI	ACCTGC	1 cut(s)	1421
BspPI	GGATC	7 cut(s)	621, 638, 1208, 1221, 1465, 1936, 2838
BspQI	GCTCTTC	1 cut(s)	2097
BspT104I	TTCGAA	1 cut(s)	234
BspTI	CTTAAG	1 cut(s)	452
BsrDI	GCAATG	2 cut(s)	559, 3038
BsrFI	RCCGGY	1 cut(s)	3205
BsrGI	TGTACA	3 cut(s)	345, 2217, 2851
BsrI	ACTGG	9 cut(s)	153, 204, 804, 883, 1459, 2386, 3124, 3243, 3252
BssAI	RCCGGY	1 cut(s)	3205
BssECI	CCNNGG	9 cut(s)	919, 920, 947, 1005, 1163, 1595, 1747, 2322, 2340
BssNAI	GTATAC	1 cut(s)	1470
BssNI	GRCGYC	2 cut(s)	5, 791
BssSI	CACGAG	2 cut(s)	2302, 2855
BssT1I	CCWWGG	6 cut(s)	1005, 1163, 1595, 1747, 2322, 2340
Bst1107I	GTATAC	1 cut(s)	1470
Bst2BI	CACGAG	2 cut(s)	2302, 2855
Bst2UI	CCWGG	4 cut(s)	35, 921, 948, 960
Bst4CI	ACNGT	8 cut(s)	1764, 1873, 2188, 2274, 2297, 2656, 2762, 2802
Bst6I	CTCTTC	1 cut(s)	2097
BstACI	GRCGYC	2 cut(s)	5, 791
BstAFI	CTTAAG	1 cut(s)	452
BstAUI	TGTACA	3 cut(s)	345, 2217, 2851
BstBI	TTCGAA	1 cut(s)	234
BstC8I	GCNNGC	1 cut(s)	2980
BstDSI	CCRYGG	2 cut(s)	1163, 2340
BstEII	GGTNACC	1 cut(s)	2326
BstENI	CCTNNNNNAGG	2 cut(s)	62, 305
BstF5I	GGATG	5 cut(s)	208, 1060, 1450, 2358, 2995
BstFNI	CGCG	2 cut(s)	913, 2978
BstH2I	RGCGCY	1 cut(s)	533
BstHHI	GCGC	3 cut(s)	532, 2004, 2417
BstMAI	GTCTC	5 cut(s)	381, 1228, 2021, 2852, 3177
BstNI	CCWGG	4 cut(s)	35, 921, 948, 960
BstNSI	RCATGY	2 cut(s)	2402, 2535
BstPI	GGTNACC	1 cut(s)	2326
BstSCI	CCNGG	4 cut(s)	33, 919, 946, 958
BstSFI	CTRYAG	2 cut(s)	1011, 2793
BstSLI	GKGCMC	1 cut(s)	1511
BstUI	CGCG	2 cut(s)	913, 2978
BstV1I	GCAGC	6 cut(s)	271, 351, 568, 674, 3023, 3088
BstV2I	GAAGAC	2 cut(s)	2559, 2693
BstX2I	RGATCY	4 cut(s)	1213, 1928, 2121, 2202
BstXI	CCANNNNNNTGG	1 cut(s)	2071
BstYI	RGATCY	4 cut(s)	1213, 1928, 2121, 2202
BstZ17I	GTATAC	1 cut(s)	1470
BsuI	GTATCC	2 cut(s)	104, 1014
BsuRI	GGCC	8 cut(s)	38, 263, 443, 470, 963, 1430, 1594, 2664
BtgI	CCRYGG	2 cut(s)	1163, 2340
BtgZI	GCGATG	1 cut(s)	1332
BtsCI	GGATG	5 cut(s)	208, 1060, 1450, 2358, 2995
BtsI	GCAGTG	2 cut(s)	750, 3138
BtsIMutI	CAGTG	7 cut(s)	20, 750, 811, 2133, 2798, 3138, 3250
BveI	ACCTGC	1 cut(s)	1421
Cac8I	GCNNGC	1 cut(s)	2980
CciI	TCATGA	1 cut(s)	1483
CfoI	GCGC	3 cut(s)	532, 2004, 2417
Cfr10I	RCCGGY	1 cut(s)	3205
Cfr13I	GGNCC	5 cut(s)	262, 442, 735, 881, 1592
CseI	GACGC	1 cut(s)	799
Csp6I	GTAC	6 cut(s)	346, 695, 1238, 2061, 2218, 2852
CviQI	GTAC	6 cut(s)	346, 695, 1238, 2061, 2218, 2852
Eam1104I	CTCTTC	1 cut(s)	2097
EarI	CTCTTC	1 cut(s)	2097
Ecl136II	GAGCTC	2 cut(s)	2107, 2733
Eco130I	CCWWGG	6 cut(s)	1005, 1163, 1595, 1747, 2322, 2340
Eco147I	AGGCCT	1 cut(s)	2664
Eco24I	GRGCYC	4 cut(s)	1037, 1250, 2109, 2735
Eco32I	GATATC	4 cut(s)	298, 1307, 1528, 2466
Eco47I	GGWCC	2 cut(s)	735, 881
Eco53kI	GAGCTC	2 cut(s)	2107, 2733
Eco57I	CTGAAG	7 cut(s)	80, 810, 915, 2337, 2571, 2801, 2910
Eco88I	CYCGRG	1 cut(s)	3173
Eco91I	GGTNACC	1 cut(s)	2326
EcoICRI	GAGCTC	2 cut(s)	2107, 2733
EcoNI	CCTNNNNNAGG	2 cut(s)	62, 305
EcoO109I	RGGNCCY	2 cut(s)	262, 735
EcoO65I	GGTNACC	1 cut(s)	2326
EcoRI	GAATTC	1 cut(s)	849
EcoRII	CCWGG	4 cut(s)	33, 919, 946, 958
EcoRV	GATATC	4 cut(s)	298, 1307, 1528, 2466
EcoT14I	CCWWGG	6 cut(s)	1005, 1163, 1595, 1747, 2322, 2340
EcoT38I	GRGCYC	4 cut(s)	1037, 1250, 2109, 2735
ErhI	CCWWGG	6 cut(s)	1005, 1163, 1595, 1747, 2322, 2340
FalI	AAGNNNNNCTT	4 cut(s)	69, 101, 215, 247
FaqI	GGGAC	2 cut(s)	1690, 3150
FauI	CCCGC	2 cut(s)	319, 807
FbaI	TGATCA	1 cut(s)	45
FblI	GTMKAC	4 cut(s)	1053, 1469, 1946, 2973
Fnu4HI	GCNGC	9 cut(s)	285, 288, 365, 557, 663, 822, 2516, 3012, 3102
FokI	GGATG	5 cut(s)	215, 1047, 1457, 2345, 3002
FriOI	GRGCYC	4 cut(s)	1037, 1250, 2109, 2735
Fsp4HI	GCNGC	9 cut(s)	285, 288, 365, 557, 663, 822, 2516, 3012, 3102
FspBI	CTAG	4 cut(s)	266, 302, 1047, 1131
FspI	TGCGCA	1 cut(s)	2003
GlaI	GCGC	3 cut(s)	531, 2003, 2416
GluI	GCNGC	9 cut(s)	285, 288, 365, 557, 663, 822, 2516, 3012, 3102
GsaI	CCCAGC	1 cut(s)	1620
GsuI	CTGGAG	2 cut(s)	2724, 3201
HaeII	RGCGCY	1 cut(s)	533
HaeIII	GGCC	8 cut(s)	38, 263, 443, 470, 963, 1430, 1594, 2664
HapII	CCGG	4 cut(s)	186, 590, 2454, 3206
HgaI	GACGC	1 cut(s)	799
HhaI	GCGC	3 cut(s)	532, 2004, 2417
Hin1I	GRCGYC	2 cut(s)	5, 791
Hin6I	GCGC	3 cut(s)	530, 2002, 2415
HinP1I	GCGC	3 cut(s)	530, 2002, 2415
HincII	GTYRAC	3 cut(s)	18, 783, 2293
HindII	GTYRAC	3 cut(s)	18, 783, 2293
HindIII	AAGCTT	4 cut(s)	92, 458, 544, 1153
HpaI	GTTAAC	1 cut(s)	18
HpaII	CCGG	4 cut(s)	186, 590, 2454, 3206
HphI	GGTGA	3 cut(s)	361, 1430, 1829
Hpy188I	TCNGA	7 cut(s)	908, 931, 1020, 2718, 2737, 2826, 2835
Hpy99I	CGWCG	1 cut(s)	2289
HpyAV	CCTTC	7 cut(s)	146, 301, 1472, 1718, 1877, 1919, 2821
HpyCH4III	ACNGT	8 cut(s)	1764, 1873, 2188, 2274, 2297, 2656, 2762, 2802
HpyCH4IV	ACGT	2 cut(s)	497, 2996
HpySE526I	ACGT	2 cut(s)	497, 2996
Hsp92I	GRCGYC	2 cut(s)	5, 791
HspAI	GCGC	3 cut(s)	530, 2002, 2415
Kpn2I	TCCGGA	2 cut(s)	589, 2453
Ksp22I	TGATCA	1 cut(s)	45
KspAI	GTTAAC	1 cut(s)	18
LguI	GCTCTTC	1 cut(s)	2097
LmnI	GCTCC	6 cut(s)	682, 1245, 1495, 1618, 2705, 2730
Lsp1109I	GCAGC	6 cut(s)	271, 351, 568, 674, 3023, 3088
LweI	GCATC	7 cut(s)	1281, 1435, 1600, 1989, 2261, 2367, 3023
MaeI	CTAG	4 cut(s)	266, 302, 1047, 1131
MaeII	ACGT	2 cut(s)	497, 2996
MaeIII	GTNAC	9 cut(s)	485, 937, 1450, 1817, 2297, 2326, 2527, 2642, 2656
MflI	RGATCY	4 cut(s)	1213, 1928, 2121, 2202
MhlI	GDGCHC	5 cut(s)	1037, 1250, 1511, 2109, 2735
MlyI	GAGTC	2 cut(s)	2854, 3185
MmeI	TCCRAC	4 cut(s)	663, 1001, 1069, 1093
MroI	TCCGGA	2 cut(s)	589, 2453
MroXI	GAANNNNTTC	3 cut(s)	84, 131, 227
MslI	CAYNNNNRTG	2 cut(s)	1634, 2363
MspA1I	CMGCKG	1 cut(s)	364
MspCI	CTTAAG	1 cut(s)	452
MspI	CCGG	4 cut(s)	186, 590, 2454, 3206
MspR9I	CCNGG	4 cut(s)	35, 921, 948, 960
Mva1269I	GAATGC	2 cut(s)	2669, 2900
MvaI	CCWGG	4 cut(s)	35, 921, 948, 960
MvnI	CGCG	2 cut(s)	913, 2978
NcoI	CCATGG	2 cut(s)	1163, 2340
NlaIV	GGNNCC	9 cut(s)	678, 736, 917, 1215, 1247, 1491, 2263, 2346, 3250
NmuCI	GTSAC	4 cut(s)	485, 1817, 2642, 2656
NsbI	TGCGCA	1 cut(s)	2003
NspI	RCATGY	2 cut(s)	2402, 2535
NspV	TTCGAA	1 cut(s)	234
PaeR7I	CTCGAG	1 cut(s)	3173
PagI	TCATGA	1 cut(s)	1483
PasI	CCCWGGG	1 cut(s)	920
PceI	AGGCCT	1 cut(s)	2664
PciSI	GCTCTTC	1 cut(s)	2097
PcsI	WCGNNNNNNNCGW	1 cut(s)	2853
PctI	GAATGC	2 cut(s)	2669, 2900
PdmI	GAANNNNTTC	3 cut(s)	84, 131, 227
PkrI	GCNGC	9 cut(s)	286, 289, 366, 558, 664, 823, 2517, 3013, 3103
PleI	GAGTC	2 cut(s)	2854, 3184
PpsI	GAGTC	2 cut(s)	2854, 3184
PpuMI	RGGWCCY	1 cut(s)	735
PshBI	ATTAAT	1 cut(s)	954
Psp124BI	GAGCTC	2 cut(s)	2109, 2735
Psp1406I	AACGTT	2 cut(s)	497, 2996
Psp5II	RGGWCCY	1 cut(s)	735
Psp6I	CCWGG	4 cut(s)	33, 919, 946, 958
PspEI	GGTNACC	1 cut(s)	2326
PspFI	CCCAGC	1 cut(s)	1616
PspGI	CCWGG	4 cut(s)	33, 919, 946, 958
PspN4I	GGNNCC	9 cut(s)	678, 736, 917, 1215, 1247, 1491, 2263, 2346, 3250
PspPI	GGNCC	5 cut(s)	262, 442, 735, 881, 1592
PspPPI	RGGWCCY	1 cut(s)	735
PspXI	VCTCGAGB	1 cut(s)	3173
PsrI	GAACNNNNNNTAC	2 cut(s)	2388, 2420
PsuI	RGATCY	4 cut(s)	1213, 1928, 2121, 2202
PvuII	CAGCTG	1 cut(s)	364
RsaI	GTAC	6 cut(s)	347, 696, 1239, 2062, 2219, 2853
RsaNI	GTAC	6 cut(s)	346, 695, 1238, 2061, 2218, 2852
RseI	CAYNNNNRTG	2 cut(s)	1634, 2363
SacI	GAGCTC	2 cut(s)	2109, 2735
SapI	GCTCTTC	1 cut(s)	2097
SatI	GCNGC	9 cut(s)	285, 288, 365, 557, 663, 822, 2516, 3012, 3102
Sau96I	GGNCC	5 cut(s)	262, 442, 735, 881, 1592
SchI	GAGTC	2 cut(s)	2854, 3185
ScrFI	CCNGG	4 cut(s)	35, 921, 948, 960
SduI	GDGCHC	5 cut(s)	1037, 1250, 1511, 2109, 2735
SfaNI	GCATC	7 cut(s)	1281, 1435, 1600, 1989, 2261, 2367, 3023
SfcI	CTRYAG	2 cut(s)	1011, 2793
Sfr274I	CTCGAG	1 cut(s)	3173
SfuI	TTCGAA	1 cut(s)	234
SinI	GGWCC	2 cut(s)	735, 881
SlaI	CTCGAG	1 cut(s)	3173
SmiMI	CAYNNNNRTG	2 cut(s)	1634, 2363
SmlI	CTYRAG	6 cut(s)	452, 2032, 2206, 2636, 2884, 3173
SmoI	CTYRAG	6 cut(s)	452, 2032, 2206, 2636, 2884, 3173
SpeI	ACTAGT	1 cut(s)	1046
SseBI	AGGCCT	1 cut(s)	2664
SspMI	CTAG	4 cut(s)	266, 302, 1047, 1131
SstI	GAGCTC	2 cut(s)	2109, 2735
StuI	AGGCCT	1 cut(s)	2664
StyD4I	CCNGG	4 cut(s)	33, 919, 946, 958
StyI	CCWWGG	6 cut(s)	1005, 1163, 1595, 1747, 2322, 2340
TaaI	ACNGT	8 cut(s)	1764, 1873, 2188, 2274, 2297, 2656, 2762, 2802
TaiI	ACGT	2 cut(s)	500, 2999
TatI	WGTACW	5 cut(s)	345, 694, 1237, 2217, 2851
TauI	GCSGC	3 cut(s)	290, 824, 2518
TscAI	CASTG	7 cut(s)	27, 757, 811, 2140, 2805, 3138, 3250
TseFI	GTSAC	4 cut(s)	485, 1817, 2642, 2656
TseI	GCWGC	6 cut(s)	284, 364, 556, 662, 3011, 3101
Tsp45I	GTSAC	4 cut(s)	485, 1817, 2642, 2656
TspRI	CASTG	7 cut(s)	27, 757, 811, 2140, 2805, 3138, 3250
Vha464I	CTTAAG	1 cut(s)	452
VpaK11BI	GGWCC	2 cut(s)	735, 881
VspI	ATTAAT	1 cut(s)	954
XagI	CCTNNNNNAGG	2 cut(s)	62, 305
XapI	RAATTY	2 cut(s)	237, 849
XbaI	TCTAGA	1 cut(s)	1130
XceI	RCATGY	2 cut(s)	2402, 2535
XhoI	CTCGAG	1 cut(s)	3173
XmiI	GTMKAC	4 cut(s)	1053, 1469, 1946, 2973
XmnI	GAANNNNTTC	3 cut(s)	84, 131, 227
XspI	CTAG	4 cut(s)	266, 302, 1047, 1131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.