Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Reverse (-)

10832913 .. 10839006

Gene Structure 6094 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011599

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3438 bp

ATGGCGACGCCAACGGCGACAACGACAACACTGACTAAAGACCTCACCTTCTTGATCATGCAATTCCTTGACGAGGACAAGTGGAGGGATACGCTTCATAAGCTTCAGCAAGAATCAGGATACTTCTTCGACATGAAGTATTTCGAGGAATTATTGCTCGAGGGAAATTGGAATGAAGTCGAGCAGTATCTTTCCGGGTTCACTAAAGTGGATGACAACCGCTATTCGATGAAGATATTCTTCGAAATTCGTAAGCAGAAATACTTAGAAGCGCTGGATAGACATGATAGAGCTGCCGCAGTAGATATCCTAGTGAAGGATCTGAAAGTCTTTGCGGGTTTTAATGAAGACTTGTACAAGGAAATCACTCAGCTGCTCACTTTGAACAATTTTAGAGACAATGCTCAACTCTCTGCTTATGCAGATATAAGGACTGCAAGAGCAATTATGGCCCAAGAACTTAAGAAGCTTGTTGAGGCCAACCCTTCTTTCCGTGAAAAATTACAGTTCCCTAGCATTAAAAGTTCAAGGCTACGGACGCTCATTAACCAAAGCTTAAATTGGCAGCATTCCCTTTGTCCTAATCCTAAACAAAATCCGGAAATAAAAACCCTATTTGTGGATCACACTTGTAGACATTCCAACGATCCATTTGCTCATTTAGCAGCAAATGGTCAGGCTCAACAGATGGGTGCAGTACAGAGAGTTGATGGCTTCATTCCTATCGGTGTAAATGGGTCCTTCCAACCCCCAATAGCACCAGTACCAATTCCTCCTCCTTTGTCAACATGGACGTCCATACCCCCCAGTGTCAATCAGTTGGCGGCTTCTGCAGGAGGTATTGGTTTTGGCAGTCCAGCAAATCCAGCTTCATATTTGAACAGTCCAGCAAATCCTGAAGATTTGTTCAGAACACGGTTCCCTGGGGTTTCAGACAGGGTTATGCTGCCAGGGGTTAATCCAGGCCACGTTCTAAGTCCAGCATTCAATGCAATTGATGAGTTTCCGAAGGCTGTTGCCCGGGTACTGAATCAGGGCTCAGTTCCCACTAGCATGGACTTCCATCCTCTTCAACAGACTATACTTCTAGTTGGAACTAGTATGGGGGATATAAGTGTCTGGGAAGTCAGTTCTAGGGAGAAGTTGGTATCAAGAAGCTTTCAGCCATGGGATATGGGGGCAAGTTCAATGACACTAAAGGCAACTTTAGTCAAGGATCCAAGTGTCTCAGTTAAGCGTGTACTATGGAGCCCTGATGGTTCTCTATTCGGAGTTGCATATTCTAAACACATGATGCAGTTATATGGTTATTTTGGTGGTGATGATGTTCGTCAGCATTTAGAGATCGATGCTCATGTTGGTAGTGTAAATGATCTGGCGTTCTGCAACCCAACTAAGCAACTCTGTGTCATAACTTGCGGTGATGATAAGGCCATCAAGGTCTGGGATGCGGCTACTGGATCAAAATTGTATACTTTTGAAGGTCATGAGGCTCCTGTTCATTCTGTGTGCCCTCATAGCAAAGAACATGTCCATTTTGTCTTTTCGACATCAGTAGATGGACAAATAAAAGCATGGTTATATGAAGTGGTGGGGCCCAGGGTTAATTACGATGCTCCCAGCCTCTCATGCACAACGATGGTTTATAGTGCAGATGGTAAAAGGCTTTTTTCATGTGGGACGAGCAAAGATGGAGAATCTCATGTTGTTGAATGGAATGAAAATGAAGGCACAATAAAGAGGAATTACCAAGGATTTCAAAAACAATCTTTGGGTGTTGTTCAATTTGACACAACCAGGAATAAGTATCTAGCAGTTGGTGACGACTATGCAATCAAAGTTTGGGATATGGATAATATCAATCTTTTGGCTACTATTGATGCTGAAGGAGGTCTACCAGCAACCCCTCGTATCCGCTTCAACAAGGAAGGATCTTTGTTAGCTGTTTCTGCAAATGACAACAGAATCAAAGTTTTAGCTACAACAGATGGTCTCCGATTGATGCGCAATTATGAAAGTCTCTCGCTTATTGCCTCTAGAAATGCACCAGAAAGGGTCAGTTCACATCGAGAGTGGTCAGTGCATATAAAGGCACTGGCTAAATCATGTGCAAAGTTGTTTCCTTCATTCTGCTTAATTCTAAATAGTTTTCTTACTTTTGCTACTTCTCATTCAATTCAATTAGGAAGTAAGTGGCTTTACTACATATTTATATCTAATGGTAGCACCAGAAGCATGGAGGATAATAAGAAACCAAAGTTAACTGAAGAGCTCAAACCTGCAAAGATCTGGAAGCCCACTGAGATTAGTGAAACTGCTCGGCTTCAGTCTTTAAGGCTCTCGACAACTACAGTTAAAACAGACAAGATCTCAAGGTTGGTTTACACTAATTCAGGTAATGCCATTCTGGCATTATCATCAAATGGAACCCATCTACTGTGGAGGTGGCCACAAGCTGACCGTAGTACGATTGGCAAGGCAACAACCAATGTTACCCCAAAATTCGTGGAACAAGCTTCAGGCATCCTGATGATCAATGATCTTACTGGTGCTAAACCTGAAGATGCAATACCTTGCTTCGCTTTGTCCAAGAATGATTCGTATGTCATGTCAGCCTCCGGAGGGAAGATATCGCTGTTCAACCTAATGACATTCAAGACGATGACAACTTTCATGTCGCCACCACCTGTGGTAACATGTCTTGCTTTCCACCCTGAAGACAATAATATAGTCGCTGTTGGATTTGATGATTCGACTATCCACATATACAATGTCCGTATCGATGAGGTTAGGATAAAGCTCAAGGGTCACTCTAAGAGAGTCACAAGCCTTGCATTCTCTCAACTGCTGAAGACACTTGTTTCTTCTGGAGCAGATGCTCAGATAGTTGTGTGGAGCTCTGATAAGTGGGAAAGGCAGAAGAACAGTTTCTTGCAGGTTCCAGCTTGGACAACACCCACAGCACTGTTTGGAACTCATCTTCAGTATCAGAAGGATCAAAATCACTTCCTCGTTGTACATGAGACTCAACTTGCTGTATACGAAACCTTGAGGCTTGAATGCCAGAAGAAGTGGGTAGTAGGAGAATCTTCAGCACCAATCTCCTATGCAACATTCTCTTGTGATAGCCAGTTAGTCTACGCGAGCTTTCTTGATGGAACTGTGTGCGTGTTTGCAGCATCGAATCTTCAAATGCAATGCCAGATTAACCCCCATGCTTATCTTCCACCTAATGTCAGCTCTGCAACATACCCCCTAGTGATAGCTGCGAATCCACAAGAGCCGAACCAGTTTGCAGTGGGACTAACAGATGGTGCAGTTGTCATGTTTGAACCACTTGAGTCTGGAGACAAATGGGGTGTGCCGCCACCAGCAGACAGTGGTTGCTTCCATGCTTCAACTTCTGCCAATGGTTCCAGTTTAGAACTCCAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1146

Amino Acids

126.96

Weight (kDa)

6.76

Isoelectric Point (pI)

36.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	13 - 40	1.8e-08	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	44 - 168	1.7e-58	TPR1-like, CTLH-containing domain
zf_topless	PF21359	171 - 211	2.5e-24	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	390 - 560	1.1e-14	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	404 - 560	1.2e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	410 - 560	6.2e-14	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	410 - 667	9.3e-24	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	417 - 526	3.9e-09	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	428 - 527	1.1e-09	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	448 - 629	5.3e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	449 - 659	4.2e-16	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	461 - 527	7e-07	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	462 - 621	1e-07	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	502 - 659	1.1e-06	WDR55
Beta-prop_WDR5	PF25175	513 - 665	1e-08	WDR5 beta-propeller domain
WD40_Prp19	PF24814	780 - 1052	5.7e-16	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	852 - 972	4.9e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	854 - 1060	7.9e-19	WDR5 beta-propeller domain
Beta-prop_WDR5	PF25175	854 - 969	3.3e-15	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	854 - 995	7.4e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	856 - 1089	2e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	859 - 1069	5e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	862 - 1063	4.7e-20	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	863 - 966	7.9e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	868 - 966	2.3e-17	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	870 - 966	7.7e-12	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	870 - 978	1.6e-09	IFT140 first beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	873 - 1016	2.2e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	875 - 969	1.5e-08	WDR90/POC16, second beta-propeller
Beta-prop_THOC3	PF25174	898 - 1073	6.7e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	2006
Acc36I	ACCTGC	2 cut(s)	2287, 2926
AccI	GTMKAC	5 cut(s)	634, 1472, 1894, 3039, 3138
AccII	CGCG	1 cut(s)	3143
AccIII	TCCGGA	2 cut(s)	598, 2618
AciI	CCGC	8 cut(s)	220, 297, 335, 824, 1419, 1451, 1915, 3365
AclWI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 3003
AcoI	YGGCCR	1 cut(s)	2447
AcsI	RAATTY	2 cut(s)	246, 2501
AcyI	GRCGYC	2 cut(s)	8, 794
AfaI	GTAC	7 cut(s)	356, 699, 765, 1026, 1242, 2467, 3018
AfeI	AGCGCT	1 cut(s)	273
AfiI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2622
AflII	CTTAAG	1 cut(s)	461
AflIII	ACRYGT	2 cut(s)	1528, 2696
AhlI	ACTAGT	1 cut(s)	1097
AjnI	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
AjuI	GAANNNNNNNTTGG	2 cut(s)	473, 505
AleI	CACNNNNGTG	1 cut(s)	206
Alw21I	GWGCWC	2 cut(s)	2274, 2900
Alw26I	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 3017, 3342
AlwI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 3003
Ama87I	CYCGRG	2 cut(s)	158, 1020
Aor13HI	TCCGGA	2 cut(s)	598, 2618
Aor51HI	AGCGCT	1 cut(s)	273
AoxI	GGCC	6 cut(s)	450, 477, 964, 1431, 1595, 2447
ApaI	GGGCCC	1 cut(s)	1599
ApeKI	GCWGC	7 cut(s)	293, 373, 565, 665, 946, 3176, 3266
ApoI	RAATTY	2 cut(s)	246, 2501
ArsI	GACNNNNNNTTYG	2 cut(s)	1515, 1547
Asp700I	GAANNNNTTC	3 cut(s)	140, 236, 2927
AspLEI	GCGC	2 cut(s)	274, 2007
AspS9I	GGNCC	4 cut(s)	451, 738, 1595, 1596
AsuC2I	CCSGG	3 cut(s)	196, 1021, 1022
AsuHPI	GGTGA	4 cut(s)	37, 1331, 1433, 1832
AsuII	TTCGAA	1 cut(s)	243
AvaI	CYCGRG	2 cut(s)	158, 1020
AvaII	GGWCC	1 cut(s)	738
BaeGI	GKGCMC	2 cut(s)	1514, 1599
BalI	TGGCCA	1 cut(s)	2449
BamHI	GGATCC	1 cut(s)	1216
BanII	GRGCYC	5 cut(s)	1040, 1253, 1599, 2274, 2900
BarI	GAAGNNNNNNTAC	4 cut(s)	1878, 1910, 2553, 2585
BbsI	GAAGAC	3 cut(s)	354, 2724, 2858
Bbv12I	GWGCWC	2 cut(s)	2274, 2900
BbvI	GCAGC	7 cut(s)	280, 360, 577, 677, 933, 3188, 3253
BceAI	ACGGC	1 cut(s)	30
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BciVI	GTATCC	3 cut(s)	82, 113, 1922
BclI	TGATCA	2 cut(s)	54, 2532
BcnI	CCSGG	3 cut(s)	196, 1021, 1022
BcoDI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 3017, 3342
BcuI	ACTAGT	1 cut(s)	1097
BfaI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3257
BfmI	CTRYAG	2 cut(s)	831, 2349
BfoI	RGCGCY	1 cut(s)	275
BfrI	CTTAAG	1 cut(s)	461
BfuAI	ACCTGC	2 cut(s)	2287, 2926
BfuI	GTATCC	3 cut(s)	82, 113, 1922
BglI	GCCNNNNNGGC	1 cut(s)	2408
BglII	AGATCT	2 cut(s)	2286, 2367
Bme1390I	CCNGG	8 cut(s)	196, 924, 951, 963, 1021, 1022, 1600, 1798
Bme18I	GGWCC	1 cut(s)	738
BmeT110I	CYCGRG	2 cut(s)	158, 1020
BmgT120I	GGNCC	4 cut(s)	451, 738, 1595, 1596
BmrFI	CCNGG	8 cut(s)	196, 924, 951, 963, 1021, 1022, 1600, 1798
BmrI	ACTGGG	1 cut(s)	801
BmuI	ACTGGG	1 cut(s)	801
BpiI	GAAGAC	3 cut(s)	354, 2724, 2858
BpmI	CTGGAG	3 cut(s)	2889, 3366, 3413
Bpu14I	TTCGAA	1 cut(s)	243
BpuEI	CTTGAG	4 cut(s)	2356, 2786, 3070, 3359
BpuMI	CCSGG	3 cut(s)	196, 1021, 1022
Bsa29I	ATCGAT	2 cut(s)	1347, 2781
BsaBI	GATNNNNATC	1 cut(s)	3000
BsaHI	GRCGYC	2 cut(s)	8, 794
BsaI	GGTCTC	1 cut(s)	1997
BsaJI	CCNNGG	8 cut(s)	922, 923, 950, 1020, 1166, 1598, 1599, 1750
BsaWI	WCCGGW	2 cut(s)	598, 2618
Bsc4I	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2622
Bse1I	ACTGG	8 cut(s)	761, 807, 1462, 2100, 2551, 3130, 3289, 3417
Bse3DI	GCAATG	1 cut(s)	3203
Bse8I	GATNNNNATC	1 cut(s)	3000
BseAI	TCCGGA	2 cut(s)	598, 2618
BseBI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BseCI	ATCGAT	2 cut(s)	1347, 2781
BseDI	CCNNGG	8 cut(s)	922, 923, 950, 1020, 1166, 1598, 1599, 1750
BseGI	GGATG	4 cut(s)	217, 1063, 1453, 2523
BseJI	GATNNNNATC	1 cut(s)	3000
BseLI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2622
BseMI	GCAATG	1 cut(s)	3203
BseMII	CTCAG	5 cut(s)	383, 1053, 1242, 2292, 2894
BseNI	ACTGG	8 cut(s)	761, 807, 1462, 2100, 2551, 3130, 3289, 3417
BseRI	GAGGAG	1 cut(s)	765
BseSI	GKGCMC	2 cut(s)	1514, 1599
BseXI	GCAGC	7 cut(s)	280, 360, 577, 677, 933, 3188, 3253
BseYI	CCCAGC	1 cut(s)	1619
BsgI	GTGCAG	3 cut(s)	714, 1671, 3336
Bsh1236I	CGCG	1 cut(s)	3143
BshFI	GGCC	6 cut(s)	452, 479, 966, 1433, 1597, 2449
BshVI	ATCGAT	2 cut(s)	1347, 2781
BsiHKAI	GWGCWC	2 cut(s)	2274, 2900
BsiHKCI	CYCGRG	2 cut(s)	158, 1020
BsiSI	CCGG	4 cut(s)	195, 599, 1021, 2619
BslFI	GGGAC	2 cut(s)	1693, 3315
BslI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2622
BsmAI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 3017, 3342
BsmFI	GGGAC	2 cut(s)	1693, 3315
BsmI	GAATGC	4 cut(s)	568, 983, 2834, 3065
BsnI	GGCC	6 cut(s)	452, 479, 966, 1433, 1597, 2449
Bso31I	GGTCTC	1 cut(s)	1997
BsoBI	CYCGRG	2 cut(s)	158, 1020
Bsp119I	TTCGAA	1 cut(s)	243
Bsp120I	GGGCCC	1 cut(s)	1595
Bsp1286I	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2274, 2900
Bsp13I	TCCGGA	2 cut(s)	598, 2618
Bsp1407I	TGTACA	2 cut(s)	354, 3016
Bsp19I	CCATGG	1 cut(s)	1166
BspACI	CCGC	8 cut(s)	220, 297, 335, 824, 1419, 1451, 1915, 3365
BspANI	GGCC	6 cut(s)	452, 479, 966, 1433, 1597, 2449
BspCNI	CTCAG	5 cut(s)	382, 1052, 1241, 2293, 2893
BspDI	ATCGAT	2 cut(s)	1347, 2781
BspEI	TCCGGA	2 cut(s)	598, 2618
BspFNI	CGCG	1 cut(s)	3143
BspHI	TCATGA	1 cut(s)	1486
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	2 cut(s)	2287, 2926
BspPI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 3003
BspQI	GCTCTTC	1 cut(s)	2262
BspT104I	TTCGAA	1 cut(s)	243
BspTI	CTTAAG	1 cut(s)	461
BspTNI	GGTCTC	1 cut(s)	1997
BsrDI	GCAATG	1 cut(s)	3203
BsrGI	TGTACA	2 cut(s)	354, 3016
BsrI	ACTGG	8 cut(s)	761, 807, 1462, 2100, 2551, 3130, 3289, 3417
BssECI	CCNNGG	8 cut(s)	922, 923, 950, 1020, 1166, 1598, 1599, 1750
BssNAI	GTATAC	2 cut(s)	1473, 3040
BssNI	GRCGYC	2 cut(s)	8, 794
BssT1I	CCWWGG	2 cut(s)	1166, 1750
Bst1107I	GTATAC	2 cut(s)	1473, 3040
Bst2UI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
Bst6I	CTCTTC	2 cut(s)	1074, 2262
BstACI	GRCGYC	2 cut(s)	8, 794
BstAFI	CTTAAG	1 cut(s)	461
BstAPI	GCANNNNNTGC	1 cut(s)	989
BstAUI	TGTACA	2 cut(s)	354, 3016
BstBI	TTCGAA	1 cut(s)	243
BstC8I	GCNNGC	1 cut(s)	3145
BstDEI	CTNAG	9 cut(s)	265, 369, 974, 1039, 1228, 1395, 2301, 2814, 2880
BstDSI	CCRYGG	1 cut(s)	1166
BstENI	CCTNNNNNAGG	2 cut(s)	71, 314
BstF5I	GGATG	4 cut(s)	217, 1063, 1453, 2523
BstFNI	CGCG	1 cut(s)	3143
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	2 cut(s)	274, 2007
BstMAI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 3017, 3342
BstNI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
BstNSI	RCATGY	2 cut(s)	1532, 2700
BstSCI	CCNGG	8 cut(s)	194, 922, 949, 961, 1019, 1020, 1598, 1796
BstSFI	CTRYAG	2 cut(s)	831, 2349
BstSLI	GKGCMC	2 cut(s)	1514, 1599
BstUI	CGCG	1 cut(s)	3143
BstV1I	GCAGC	7 cut(s)	280, 360, 577, 677, 933, 3188, 3253
BstV2I	GAAGAC	3 cut(s)	354, 2724, 2858
BstX2I	RGATCY	5 cut(s)	319, 1216, 1931, 2286, 2367
BstXI	CCANNNNNNTGG	2 cut(s)	1054, 2236
BstYI	RGATCY	5 cut(s)	319, 1216, 1931, 2286, 2367
BstZ17I	GTATAC	2 cut(s)	1473, 3040
Bsu15I	ATCGAT	2 cut(s)	1347, 2781
BsuI	GTATCC	3 cut(s)	82, 113, 1922
BsuRI	GGCC	6 cut(s)	452, 479, 966, 1433, 1597, 2449
BsuTUI	ATCGAT	2 cut(s)	1347, 2781
BtgI	CCRYGG	1 cut(s)	1166
BtsCI	GGATG	4 cut(s)	217, 1063, 1453, 2523
BtsI	GCAGTG	1 cut(s)	3303
BtsIMutI	CAGTG	8 cut(s)	29, 814, 2085, 2093, 2298, 2963, 3303, 3385
BveI	ACCTGC	2 cut(s)	2287, 2926
Cac8I	GCNNGC	1 cut(s)	3145
CciI	TCATGA	1 cut(s)	1486
CfoI	GCGC	2 cut(s)	274, 2007
Cfr13I	GGNCC	4 cut(s)	451, 738, 1595, 1596
Cfr9I	CCCGGG	1 cut(s)	1020
ClaI	ATCGAT	2 cut(s)	1347, 2781
CseI	GACGC	2 cut(s)	16, 547
Csp6I	GTAC	7 cut(s)	355, 698, 764, 1025, 1241, 2466, 3017
CspCI	CAANNNNNGTGG	2 cut(s)	2487, 2522
CviQI	GTAC	7 cut(s)	355, 698, 764, 1025, 1241, 2466, 3017
DdeI	CTNAG	9 cut(s)	265, 369, 974, 1039, 1228, 1395, 2301, 2814, 2880
EaeI	YGGCCR	1 cut(s)	2447
Eam1104I	CTCTTC	2 cut(s)	1074, 2262
EarI	CTCTTC	2 cut(s)	1074, 2262
Ecl136II	GAGCTC	2 cut(s)	2272, 2898
Eco130I	CCWWGG	2 cut(s)	1166, 1750
Eco24I	GRGCYC	5 cut(s)	1040, 1253, 1599, 2274, 2900
Eco31I	GGTCTC	1 cut(s)	1997
Eco32I	GATATC	2 cut(s)	307, 2631
Eco47I	GGWCC	1 cut(s)	738
Eco47III	AGCGCT	1 cut(s)	273
Eco53kI	GAGCTC	2 cut(s)	2272, 2898
Eco88I	CYCGRG	2 cut(s)	158, 1020
EcoICRI	GAGCTC	2 cut(s)	2272, 2898
EcoNI	CCTNNNNNAGG	2 cut(s)	71, 314
EcoO109I	RGGNCCY	2 cut(s)	738, 1595
EcoRII	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
EcoRV	GATATC	2 cut(s)	307, 2631
EcoT14I	CCWWGG	2 cut(s)	1166, 1750
EcoT38I	GRGCYC	5 cut(s)	1040, 1253, 1599, 2274, 2900
ErhI	CCWWGG	2 cut(s)	1166, 1750
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	2 cut(s)	1693, 3315
FauI	CCCGC	1 cut(s)	328
FbaI	TGATCA	2 cut(s)	54, 2532
FblI	GTMKAC	5 cut(s)	634, 1472, 1894, 3039, 3138
FokI	GGATG	4 cut(s)	224, 1050, 1460, 2510
FriOI	GRGCYC	5 cut(s)	1040, 1253, 1599, 2274, 2900
FspBI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3257
FspI	TGCGCA	1 cut(s)	2006
GlaI	GCGC	2 cut(s)	273, 2006
GsaI	CCCAGC	1 cut(s)	1623
GsuI	CTGGAG	3 cut(s)	2889, 3366, 3413
HaeII	RGCGCY	1 cut(s)	275
HaeIII	GGCC	6 cut(s)	452, 479, 966, 1433, 1597, 2449
HapII	CCGG	4 cut(s)	195, 599, 1021, 2619
HgaI	GACGC	2 cut(s)	16, 547
HhaI	GCGC	2 cut(s)	274, 2007
Hin1I	GRCGYC	2 cut(s)	8, 794
Hin6I	GCGC	2 cut(s)	272, 2005
HinP1I	GCGC	2 cut(s)	272, 2005
HincII	GTYRAC	2 cut(s)	786, 2262
HindII	GTYRAC	2 cut(s)	786, 2262
HindIII	AAGCTT	5 cut(s)	101, 467, 553, 1156, 2514
HpaI	GTTAAC	1 cut(s)	2262
HpaII	CCGG	4 cut(s)	195, 599, 1021, 2619
HphI	GGTGA	4 cut(s)	37, 1331, 1433, 1832
Hpy188I	TCNGA	9 cut(s)	324, 911, 934, 1008, 1271, 1997, 2883, 2902, 2991
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4IV	ACGT	2 cut(s)	794, 969
HpyF3I	CTNAG	9 cut(s)	265, 369, 974, 1039, 1228, 1395, 2301, 2814, 2880
HpySE526I	ACGT	2 cut(s)	794, 969
Hsp92I	GRCGYC	2 cut(s)	8, 794
HspAI	GCGC	2 cut(s)	272, 2005
Kpn2I	TCCGGA	2 cut(s)	598, 2618
Ksp22I	TGATCA	2 cut(s)	54, 2532
KspAI	GTTAAC	1 cut(s)	2262
LguI	GCTCTTC	1 cut(s)	2262
LmnI	GCTCC	5 cut(s)	1248, 1498, 1621, 2870, 2895
Lsp1109I	GCAGC	7 cut(s)	280, 360, 577, 677, 933, 3188, 3253
MaeI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3257
MaeII	ACGT	2 cut(s)	794, 969
MaeIII	GTNAC	5 cut(s)	1820, 2491, 2692, 2807, 2821
MfeI	CAATTG	1 cut(s)	993
MflI	RGATCY	5 cut(s)	319, 1216, 1931, 2286, 2367
MhlI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2274, 2900
MlsI	TGGCCA	1 cut(s)	2449
MluNI	TGGCCA	1 cut(s)	2449
MlyI	GAGTC	3 cut(s)	2829, 3019, 3350
MmeI	TCCRAC	4 cut(s)	666, 769, 1072, 2719
Mox20I	TGGCCA	1 cut(s)	2449
MroI	TCCGGA	2 cut(s)	598, 2618
MroXI	GAANNNNTTC	3 cut(s)	140, 236, 2927
MscI	TGGCCA	1 cut(s)	2449
MslI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2528
Msp20I	TGGCCA	1 cut(s)	2449
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	4 cut(s)	195, 599, 1021, 2619
MspR9I	CCNGG	8 cut(s)	196, 924, 951, 963, 1021, 1022, 1600, 1798
MunI	CAATTG	1 cut(s)	993
Mva1269I	GAATGC	4 cut(s)	568, 983, 2834, 3065
MvaI	CCWGG	5 cut(s)	924, 951, 963, 1600, 1798
MvnI	CGCG	1 cut(s)	3143
NciI	CCSGG	3 cut(s)	196, 1021, 1022
NcoI	CCATGG	1 cut(s)	1166
NmeAIII	GCCGAG	1 cut(s)	2299
NmuCI	GTSAC	3 cut(s)	1820, 2807, 2821
NsbI	TGCGCA	1 cut(s)	2006
NspI	RCATGY	2 cut(s)	1532, 2700
NspV	TTCGAA	1 cut(s)	243
OliI	CACNNNNGTG	1 cut(s)	206
PaeR7I	CTCGAG	1 cut(s)	158
PagI	TCATGA	1 cut(s)	1486
PasI	CCCWGGG	2 cut(s)	923, 1599
PciI	ACATGT	2 cut(s)	1528, 2696
PciSI	GCTCTTC	1 cut(s)	2262
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 20
PctI	GAATGC	4 cut(s)	568, 983, 2834, 3065
PdmI	GAANNNNTTC	3 cut(s)	140, 236, 2927
PfeI	GAWTC	9 cut(s)	113, 1030, 1697, 1965, 2597, 2750, 3086, 3184, 3271
PleI	GAGTC	3 cut(s)	2828, 3019, 3349
PpsI	GAGTC	3 cut(s)	2828, 3019, 3349
PpuMI	RGGWCCY	1 cut(s)	738
PscI	ACATGT	2 cut(s)	1528, 2696
Psp124BI	GAGCTC	2 cut(s)	2274, 2900
Psp5II	RGGWCCY	1 cut(s)	738
Psp6I	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
PspFI	CCCAGC	1 cut(s)	1619
PspGI	CCWGG	5 cut(s)	922, 949, 961, 1598, 1796
PspOMI	GGGCCC	1 cut(s)	1595
PspPI	GGNCC	4 cut(s)	451, 738, 1595, 1596
PspPPI	RGGWCCY	1 cut(s)	738
PspXI	VCTCGAGB	1 cut(s)	158
PstI	CTGCAG	1 cut(s)	835
PsuI	RGATCY	5 cut(s)	319, 1216, 1931, 2286, 2367
PvuII	CAGCTG	1 cut(s)	373
RsaI	GTAC	7 cut(s)	356, 699, 765, 1026, 1242, 2467, 3018
RsaNI	GTAC	7 cut(s)	355, 698, 764, 1025, 1241, 2466, 3017
RseI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2528
SacI	GAGCTC	2 cut(s)	2274, 2900
SapI	GCTCTTC	1 cut(s)	2262
Sau96I	GGNCC	4 cut(s)	451, 738, 1595, 1596
SchI	GAGTC	3 cut(s)	2829, 3019, 3350
ScrFI	CCNGG	8 cut(s)	196, 924, 951, 963, 1021, 1022, 1600, 1798
SduI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1599, 2274, 2900
SfcI	CTRYAG	2 cut(s)	831, 2349
Sfr274I	CTCGAG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	243
SinI	GGWCC	1 cut(s)	738
SlaI	CTCGAG	1 cut(s)	158
SmaI	CCCGGG	1 cut(s)	1022
SmiMI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2528
SmlI	CTYRAG	6 cut(s)	158, 461, 2371, 2801, 3049, 3338
SmoI	CTYRAG	6 cut(s)	158, 461, 2371, 2801, 3049, 3338
SpeI	ACTAGT	1 cut(s)	1097
SsiI	CCGC	8 cut(s)	220, 297, 335, 824, 1419, 1451, 1915, 3365
SspMI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3257
SstI	GAGCTC	2 cut(s)	2274, 2900
StyD4I	CCNGG	8 cut(s)	194, 922, 949, 961, 1019, 1020, 1598, 1796
StyI	CCWWGG	2 cut(s)	1166, 1750
TaiI	ACGT	2 cut(s)	797, 972
TatI	WGTACW	4 cut(s)	354, 697, 1240, 3016
TauI	GCSGC	4 cut(s)	299, 827, 1454, 3367
TfiI	GAWTC	9 cut(s)	113, 1030, 1697, 1965, 2597, 2750, 3086, 3184, 3271
TscAI	CASTG	8 cut(s)	36, 814, 2085, 2100, 2305, 2970, 3303, 3385
TseFI	GTSAC	3 cut(s)	1820, 2807, 2821
TseI	GCWGC	7 cut(s)	293, 373, 565, 665, 946, 3176, 3266
Tsp45I	GTSAC	3 cut(s)	1820, 2807, 2821
TspGWI	ACGGA	3 cut(s)	482, 550, 2765
TspMI	CCCGGG	1 cut(s)	1020
TspRI	CASTG	8 cut(s)	36, 814, 2085, 2100, 2305, 2970, 3303, 3385
Vha464I	CTTAAG	1 cut(s)	461
VpaK11BI	GGWCC	1 cut(s)	738
XagI	CCTNNNNNAGG	2 cut(s)	71, 314
XapI	RAATTY	2 cut(s)	246, 2501
XbaI	TCTAGA	1 cut(s)	2036
XceI	RCATGY	2 cut(s)	1532, 2700
XhoI	CTCGAG	1 cut(s)	158
XmaI	CCCGGG	1 cut(s)	1020
XmiI	GTMKAC	5 cut(s)	634, 1472, 1894, 3039, 3138
XmnI	GAANNNNTTC	3 cut(s)	140, 236, 2927
XspI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 2037, 3257
ZraI	GACGTC	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.