Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Reverse (-)

25978677 .. 25990821

Gene Structure 12145 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1222700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3405 bp

ATGTCTTCTCTGAGTAGAGAGCTTGTGTTCTTGATCTTGCAATTTCTCGATGAGGAAAAATTCAAAGAGACTGTTCACAAGCTAGAGCAGGAATCTGGGTTTTTCTTTAATGTGAAATACTTTGAGGATGAGGTGCATAATGGCAATTGGGATGAAGTTGAGAAGTACCTCTCCGGGTTCACTAAAGTGGACGATAACCGCTATTCCATGAAAATCTTTTTCGAGATAAGGAAGCAGAAGTATCTCGAGGCATTGGACAAGCATGATCGGTCCAAAGCCGTGGATATTCTAGTAAAGGATTTAAAGGTTTTTGCCACATTCAATGAAGAACTTTTCAAGGAAATCACTCAGCTCTTGACATTGGAGAACTTTAGGGAGAATGAACAACTATCCAAGTATGGAGATACAAAGTCTGCCAGGGCAATCATGTTGGTTGAACTTAAGAAACTGATTGAAGCAAATCCCTTATTCCGTGATAAATTGCAGTTCCCAAGCCTTAAGAATTCGAGGCTACGGACACTCATTAACCAAAGCTTAAATTGGCAGCATCAGCTTTGTAAGAACCCAAGACCAAATCCAGATATAAAAACACTTTTTGTGGATCACTCATGTGGACAACCTAATGGTACGCGGGCACCATCACCTGCAAACAACCCACTGCTTGGATCCTTACCAAAAGCTGGAGGGTTTCCTCCACTAGGTGCACATGGGCCTTTTCAACCTACACCAGCCCCGGTTCCCATACCCCTTGCAGGTTGGATGTCTAATCCTTCCACTGTTACTCATCCAGCAGTTTCTGAAGGAGGAGGTATTGGTCTTGGTGGTCCATCAATAACAGCTGCCTTGAAACATCCAAGGACTCCTCCAACTAATCCTTCTGTGGAGTATCCGTCTGGGGATTCTGATCATGTTTCTAAAAGAACAAGGCCGATGGGGCTGTCTACCGAGGTTAATCTCCCTGTTAACATGTTGCCAGTAACATTTCCAGGGCACGGTCATGGTCAACCTTTGAATGCACCTGATGACTTGCCCAAGAATGTCACACTGACTTTGAATCAGGGTTCATCTCCCATGAGCATGGATTTTCATCCTTCACAGCAGACCTTGCTTCTTGTTGGTACCAATGTGGGGGACATAGGGCTGTGGGAAGTTGGATCTAAGGAGCGACTAGTTTTAAGGAACTTCAAAGTATGGGATCTTAGTTCGTGCTCAATGCCCCTGCAGGCTGCTCTAGTTAAGGACCCTGGTGTGTCTGTTAACCGTGTGATTTGGAGCCCTGATGGTGCTTTGTTTGGTGTTGCTTACTCAAGGCACATTGTTCAAATATATTCATATCATGGGGGTGATGACGTACGACAGCACCTAGAGATTGATGCACATGTTGGTGGAGTAAATGATATAGCATTCTCCCATCCCAATAAGCAGCTTTGTGTGATTACCTGTGGGGATGACAAGACCATCAAGGTGTGGGATGCTACAACTGGTGCAAAACAATATACTTTTGAAGGTCATGAGGCTCCAGTTTATTCTGTCTGCCCACATTATAAGGAAAACATTCAGTTTATCTTTTCAACAGCACTGGATGGAAAGATAAAGGCTTGGTTGTATGACAATTTGGGATCTCGAGTTGATTATGATGCTCCTGGTCGCTGGTGCACAACCATGGCTTATAGTGCTGATGGTACAAGGCTATTTTCATGTGGGACAAGTAAAGATGGTGAGTCATATATTGTTGAATGGAATGAAAGTGAAGGGGCTGTGAAAAGGACCTATCAAGGCTTTCGCAAGCGTTCTTTTGGCGTTGTGCAATTCGATACTACTAAAAACCGGTTTTTGGCTGCTGGTGATGATTTTTCTATTAAATTTTGGGACATGGACAATATTCAACTTCTGACAACTGTTGATGCTGATGGAGGCCTCCCAGCAAGCCCTCGTATCCGCTTTAACAAGGATGGCACTCTCTTGGCTGTTTCTGCCAATGAGAATGGGATTAAAGTTTTGGCAAATGCAGATGGAATGCGGTTGTTGCGAACATTTGAGAATCATCTCTCTTATGATGCGTCAAGGCCCTCTGAAGTTGTGACAAAGCCTGCAATAACCCCAATTCAAGTTGCAGCAGCAGCTGCTAGTGGTGCTGGACTGGCTGAAAGAAGTGCATCTGCAGCTGCTGTTTCTGGAATGAATGGGGATGCTCGGAACTTAGGGGATGTGAAACCTAGAATAGCGGAAGAATCCAATGACAAATCAAAGATTTGGAAACTCACTGAAATCAGCGAACCATCACAATGCCGTTCCTTGAGGCTACCTGAAAACATGAGAGTAACCAAGATTTCGAGGTTAATCTATACTAATTCGGGAAGTGCAATTTTGGCTTTAGCATCAAATGCCATCCATTTGTTATGGAAATGGCAGAGGAGTGAGCGTAACTCTAGCAAGGCAACTGCTAGTGTTTCACCTCAACTGTGGCAACCCTCAAGTGGCATTTTAATGACCAATGATATTACTGACACAAGTCCTGAAGAAGCTGTTCCTTGCTTTGCTTTGTCCAAGAATGATTCTTATGTAATGTCAGCATCTGGTGGAAAGATTTCCCTGTTCAATATGATGACATTTAAGACAATGACAACTTTCATGCCTCCACCACCTGCTGCAACATTTCTTGCTTTCCATCCTCAAGATAACAATATTATAGCTATTGGGATGGATGATTCTACAATTCAGATATATAATGTCCGCGTAGATGAGGTTAAGAGCAAGCTTAAAGGCCACTCTAAAAGAATAACTGGCCTAGCATTTTCTCATGTACTGAATGCGCTAGTTTCGTCAGGAGCAGATGCTCAGCTCTGCGTATGGAACTCTGATGGATGGGAAAAGCTGAAGTCCAGATTCTTGCAGCTTCCAGCTGGGAGAACAACAGCTTCACTGTCAGACACACGTGTACAGTTTCATCACAACCAGCAACAGTTCTTGGTTGTGCACGAGACTCAACTTGCCATATATGAAACAACAAAACTAGAATGTGTAAAGCAGTGGGTTCCACGTGATTCTGCTGCACCAATCTCTCATGCAACATTCTCATGCGATAGCCAGCTTGTATATGCCAGCTTTTTAGATGCAACCGTGTGTGTGTTCAGCGCTGCAAATCTCAGACTGCGGTGTCGTATTAATCCTTCTGCTTATCTTCCTGCTAATGTTAGTTCTAACGTACAGCCCCTTGTTATTGCGGCGCATCCACAAGAAGCAAATCAGTTTGCATTAGGACTATCGGATGGTGCTGTTCACGTCTTTGAGCCCCTTGAATCTGAAGGCAAATGGGGTGTGGCTCCACCTGTTGAAAATGGTTCAGCAAGCAGTGCGCCGGCTACTCAAGTTGGAACTTCAGGTTCAGATCAAGCTCAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

1135

Amino Acids

124.63

Weight (kDa)

6.68

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	6.8e-13	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	1.4e-63	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	7e-25	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	394 - 566	3.7e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	417 - 568	2e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	418 - 630	5.8e-23	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	418 - 676	7.9e-27	THOC3 beta-propeller domain
WDR55	PF24796	419 - 534	5.2e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	419 - 571	1.7e-09	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	431 - 537	7.6e-12	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	455 - 634	5.9e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	457 - 491	3.1e-06	WD domain, G-beta repeat
WD40_Prp19	PF24814	463 - 537	4.4e-07	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	471 - 589	3.1e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	523 - 678	5.9e-07	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	528 - 666	1.9e-06	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	577 - 682	2.7e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	814 - 1038	9e-17	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	820 - 1056	1.6e-11	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	835 - 958	7e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	837 - 953	8.7e-15	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	838 - 961	6.5e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	838 - 1048	1.8e-17	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	840 - 952	7.4e-09	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	840 - 952	8e-06	WDR55
Beta-prop_WDR5	PF25175	840 - 1048	2.4e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	844 - 1041	3.3e-12	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	856 - 962	5.5e-09	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	857 - 952	6.6e-09	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	861 - 954	3.3e-07	WDR90/POC16, second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1545
AarI	CACCTGC	2 cut(s)	652, 2653
AasI	GACNNNNNNGTC	1 cut(s)	3156
Acc36I	ACCTGC	3 cut(s)	652, 743, 2653
Acc65I	GGTACC	1 cut(s)	1118
AccB1I	GGYRCC	2 cut(s)	634, 1118
AccB7I	CCANNNNNTGG	2 cut(s)	662, 680
AccI	GTMKAC	1 cut(s)	941
AccII	CGCG	2 cut(s)	631, 2736
AciI	CCGC	8 cut(s)	199, 631, 1939, 2020, 2225, 2734, 3154, 3224
AclWI	GGATC	6 cut(s)	609, 660, 673, 1162, 1203, 1627
AcsI	RAATTY	3 cut(s)	59, 502, 1862
AcuI	CTGAAG	6 cut(s)	819, 2094, 2538, 2897, 3324, 3363
AcvI	CACGTG	2 cut(s)	2936, 3041
AdeI	CACNNNGTG	1 cut(s)	701
AfaI	GTAC	8 cut(s)	167, 628, 1120, 1353, 1684, 2805, 2940, 3207
AfeI	AGCGCT	1 cut(s)	3136
AfiI	CCNNNNNNNGG	8 cut(s)	662, 680, 698, 752, 992, 1128, 1834, 2477
AflII	CTTAAG	2 cut(s)	440, 497
AflIII	ACRYGT	4 cut(s)	966, 1378, 2933, 2935
AgeI	ACCGGT	1 cut(s)	1827
AhlI	ACTAGT	1 cut(s)	1168
AjiI	CACGTC	1 cut(s)	3283
AjnI	CCWGG	4 cut(s)	416, 985, 1243, 1642
AjuI	GAANNNNNNNTTGG	2 cut(s)	859, 891
AleI	CACNNNNGTG	2 cut(s)	185, 609
Alw21I	GWGCWC	4 cut(s)	706, 1211, 1658, 2979
Alw26I	GTCTC	2 cut(s)	62, 2975
Alw44I	GTGCAC	3 cut(s)	702, 1654, 2975
AlwI	GGATC	6 cut(s)	609, 660, 673, 1162, 1203, 1627
AlwNI	CAGNNNCTG	4 cut(s)	797, 2123, 2168, 2576
Ama87I	CYCGRG	2 cut(s)	245, 1623
Aor51HI	AGCGCT	1 cut(s)	3136
AoxI	GGCC	6 cut(s)	710, 926, 1915, 2066, 2764, 2785
ApaLI	GTGCAC	3 cut(s)	702, 1654, 2975
ApoI	RAATTY	3 cut(s)	59, 502, 1862
AseI	ATTAAT	1 cut(s)	3165
AsiGI	ACCGGT	1 cut(s)	1827
Asp700I	GAANNNNTTC	3 cut(s)	1554, 2526, 2587
Asp718I	GGTACC	1 cut(s)	1118
AspLEI	GCGC	4 cut(s)	2815, 3137, 3229, 3358
AspS9I	GGNCC	6 cut(s)	270, 710, 824, 1240, 1767, 2067
AsuC2I	CCSGG	2 cut(s)	175, 734
AsuHPI	GGTGA	5 cut(s)	633, 1355, 1730, 1856, 2445
AvaI	CYCGRG	2 cut(s)	245, 1623
AvaII	GGWCC	4 cut(s)	270, 824, 1240, 1767
BaeGI	GKGCMC	5 cut(s)	637, 706, 993, 1658, 2979
BaeI	ACNNNNGTAYC	2 cut(s)	1110, 1143
BamHI	GGATCC	1 cut(s)	665
BanI	GGYRCC	2 cut(s)	634, 1118
BanII	GRGCYC	2 cut(s)	1277, 3294
BarI	GAAGNNNNNNTAC	2 cut(s)	3154, 3186
BauI	CACGAG	1 cut(s)	2978
BbrPI	CACGTG	2 cut(s)	2936, 3041
Bbv12I	GWGCWC	4 cut(s)	706, 1211, 1658, 2979
BceAI	ACGGC	2 cut(s)	263, 2274
BciT130I	CCWGG	4 cut(s)	418, 987, 1245, 1644
BciVI	GTATCC	2 cut(s)	897, 1946
BclI	TGATCA	1 cut(s)	904
BcnI	CCSGG	2 cut(s)	175, 734
BcoDI	GTCTC	2 cut(s)	62, 2975
BcuI	ACTAGT	1 cut(s)	1168
BfmI	CTRYAG	2 cut(s)	1220, 2160
BfoI	RGCGCY	1 cut(s)	3138
BfrI	CTTAAG	2 cut(s)	440, 497
BfuAI	ACCTGC	3 cut(s)	652, 743, 2653
BfuI	GTATCC	2 cut(s)	897, 1946
BglI	GCCNNNNNGGC	1 cut(s)	934
BlpI	GCTNAGC	1 cut(s)	2838
Bme1390I	CCNGG	6 cut(s)	175, 418, 734, 987, 1245, 1644
Bme18I	GGWCC	4 cut(s)	270, 824, 1240, 1767
BmeT110I	CYCGRG	2 cut(s)	245, 1623
BmgBI	CACGTC	1 cut(s)	3283
BmgT120I	GGNCC	6 cut(s)	270, 710, 824, 1240, 1767, 2067
BmiI	GGNNCC	9 cut(s)	636, 667, 738, 1120, 1242, 1274, 1518, 3036, 3324
BmrFI	CCNGG	6 cut(s)	175, 418, 734, 987, 1245, 1644
BoxI	GACNNNNGTC	1 cut(s)	2511
BplI	GAGNNNNNCTC	2 cut(s)	2411, 2443
BpmI	CTGGAG	2 cut(s)	702, 1503
Bpu1102I	GCTNAGC	1 cut(s)	2838
BpuEI	CTTGAG	5 cut(s)	1291, 2317, 2458, 2658, 3351
BpuMI	CCSGG	2 cut(s)	175, 734
BsaAI	YACGTR	2 cut(s)	2936, 3041
BsaBI	GATNNNNATC	2 cut(s)	903, 1086
BsaJI	CCNNGG	8 cut(s)	279, 417, 732, 854, 945, 986, 1243, 1662
BsaWI	WCCGGW	1 cut(s)	1827
BsaXI	ACNNNNNCTCC	4 cut(s)	875, 905, 2407, 2437
Bsc4I	CCNNNNNNNGG	8 cut(s)	662, 680, 698, 752, 992, 1128, 1834, 2477
Bse118I	RCCGGY	2 cut(s)	1827, 3358
Bse1I	ACTGG	6 cut(s)	974, 1486, 1520, 1584, 2145, 2788
Bse8I	GATNNNNATC	2 cut(s)	903, 1086
BseBI	CCWGG	4 cut(s)	418, 987, 1245, 1644
BseDI	CCNNGG	8 cut(s)	279, 417, 732, 854, 945, 986, 1243, 1662
BseJI	GATNNNNATC	2 cut(s)	903, 1086
BseLI	CCNNNNNNNGG	8 cut(s)	662, 680, 698, 752, 992, 1128, 1834, 2477
BseMII	CTCAG	3 cut(s)	362, 2852, 3160
BseNI	ACTGG	6 cut(s)	974, 1486, 1520, 1584, 2145, 2788
BseRI	GAGGAG	3 cut(s)	819, 852, 2428
BseSI	GKGCMC	5 cut(s)	637, 706, 993, 1658, 2979
BseYI	CCCAGC	2 cut(s)	1921, 2903
BsgI	GTGCAG	1 cut(s)	3036
Bsh1236I	CGCG	2 cut(s)	631, 2736
BshFI	GGCC	6 cut(s)	712, 928, 1917, 2068, 2766, 2787
BshNI	GGYRCC	2 cut(s)	634, 1118
BshTI	ACCGGT	1 cut(s)	1827
BsiHKAI	GWGCWC	4 cut(s)	706, 1211, 1658, 2979
BsiHKCI	CYCGRG	2 cut(s)	245, 1623
BsiSI	CCGG	4 cut(s)	174, 734, 1828, 3359
BsiWI	CGTACG	1 cut(s)	1351
BslFI	GGGAC	3 cut(s)	1145, 1717, 1883
BslI	CCNNNNNNNGG	8 cut(s)	662, 680, 698, 752, 992, 1128, 1834, 2477
BsmAI	GTCTC	2 cut(s)	62, 2975
BsmFI	GGGAC	3 cut(s)	1145, 1717, 1883
BsmI	GAATGC	4 cut(s)	1018, 1403, 2022, 2815
BsnI	GGCC	6 cut(s)	712, 928, 1917, 2068, 2766, 2787
BsoBI	CYCGRG	2 cut(s)	245, 1623
Bsp1286I	GDGCHC	8 cut(s)	637, 706, 993, 1211, 1277, 1658, 2979, 3294
Bsp1407I	TGTACA	1 cut(s)	2938
Bsp143I	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
Bsp1720I	GCTNAGC	1 cut(s)	2838
Bsp19I	CCATGG	1 cut(s)	1662
BspACI	CCGC	8 cut(s)	199, 631, 1939, 2020, 2225, 2734, 3154, 3224
BspANI	GGCC	6 cut(s)	712, 928, 1917, 2068, 2766, 2787
BspCNI	CTCAG	3 cut(s)	361, 2851, 3159
BspFNI	CGCG	2 cut(s)	631, 2736
BspHI	TCATGA	1 cut(s)	1510
BspLI	GGNNCC	9 cut(s)	636, 667, 738, 1120, 1242, 1274, 1518, 3036, 3324
BspMAI	CTGCAG	2 cut(s)	1224, 2164
BspMI	ACCTGC	3 cut(s)	652, 743, 2653
BspPI	GGATC	6 cut(s)	609, 660, 673, 1162, 1203, 1627
BspT107I	GGYRCC	2 cut(s)	634, 1118
BspTI	CTTAAG	2 cut(s)	440, 497
BsrFI	RCCGGY	2 cut(s)	1827, 3358
BsrGI	TGTACA	1 cut(s)	2938
BsrI	ACTGG	6 cut(s)	974, 1486, 1520, 1584, 2145, 2788
BssAI	RCCGGY	2 cut(s)	1827, 3358
BssECI	CCNNGG	8 cut(s)	279, 417, 732, 854, 945, 986, 1243, 1662
BssMI	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
BssSI	CACGAG	1 cut(s)	2978
BssT1I	CCWWGG	2 cut(s)	854, 1662
Bst2BI	CACGAG	1 cut(s)	2978
Bst2UI	CCWGG	4 cut(s)	418, 987, 1245, 1644
BstAFI	CTTAAG	2 cut(s)	440, 497
BstAPI	GCANNNNNTGC	4 cut(s)	1105, 2123, 2384, 3353
BstAUI	TGTACA	1 cut(s)	2938
BstBAI	YACGTR	2 cut(s)	2936, 3041
BstDEI	CTNAG	8 cut(s)	11, 348, 1158, 1199, 2200, 2838, 3146, 3396
BstDSI	CCRYGG	2 cut(s)	279, 1662
BstENI	CCTNNNNNAGG	1 cut(s)	696
BstFNI	CGCG	2 cut(s)	631, 2736
BstH2I	RGCGCY	1 cut(s)	3138
BstHHI	GCGC	4 cut(s)	2815, 3137, 3229, 3358
BstKTI	GATC	9 cut(s)	36, 268, 604, 668, 907, 1157, 1198, 1622, 3391
BstMAI	GTCTC	2 cut(s)	62, 2975
BstMBI	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
BstNI	CCWGG	4 cut(s)	418, 987, 1245, 1644
BstNSI	RCATGY	2 cut(s)	970, 1382
BstPAI	GACNNNNGTC	1 cut(s)	2511
BstSCI	CCNGG	6 cut(s)	173, 416, 732, 985, 1243, 1642
BstSFI	CTRYAG	2 cut(s)	1220, 2160
BstSLI	GKGCMC	5 cut(s)	637, 706, 993, 1658, 2979
BstUI	CGCG	2 cut(s)	631, 2736
BstX2I	RGATCY	4 cut(s)	665, 1154, 1195, 1619
BstXI	CCANNNNNNTGG	2 cut(s)	280, 1078
BstYI	RGATCY	4 cut(s)	665, 1154, 1195, 1619
BsuI	GTATCC	2 cut(s)	897, 1946
BsuRI	GGCC	6 cut(s)	712, 928, 1917, 2068, 2766, 2787
BtgI	CCRYGG	2 cut(s)	279, 1662
BtrI	CACGTC	1 cut(s)	3283
BtsI	GCAGTG	3 cut(s)	656, 3035, 3358
BtsIMutI	CAGTG	8 cut(s)	656, 774, 1043, 1577, 2262, 2921, 3035, 3358
BveI	ACCTGC	3 cut(s)	652, 743, 2653
CaiI	CAGNNNCTG	4 cut(s)	797, 2123, 2168, 2576
CciI	TCATGA	1 cut(s)	1510
CfoI	GCGC	4 cut(s)	2815, 3137, 3229, 3358
Cfr10I	RCCGGY	2 cut(s)	1827, 3358
Cfr13I	GGNCC	6 cut(s)	270, 710, 824, 1240, 1767, 2067
CseI	GACGC	1 cut(s)	2049
Csp6I	GTAC	8 cut(s)	166, 627, 1119, 1352, 1683, 2804, 2939, 3206
CspAI	ACCGGT	1 cut(s)	1827
CviQI	GTAC	8 cut(s)	166, 627, 1119, 1352, 1683, 2804, 2939, 3206
DdeI	CTNAG	8 cut(s)	11, 348, 1158, 1199, 2200, 2838, 3146, 3396
DpnI	GATC	9 cut(s)	35, 267, 603, 667, 906, 1156, 1197, 1621, 3390
DpnII	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
DraI	TTTAAA	1 cut(s)	303
DraIII	CACNNNGTG	1 cut(s)	701
DrdI	GACNNNNNNGTC	1 cut(s)	3156
DseDI	GACNNNNNNGTC	1 cut(s)	3156
Eco130I	CCWWGG	2 cut(s)	854, 1662
Eco147I	AGGCCT	1 cut(s)	1917
Eco24I	GRGCYC	2 cut(s)	1277, 3294
Eco47I	GGWCC	4 cut(s)	270, 824, 1240, 1767
Eco47III	AGCGCT	1 cut(s)	3136
Eco57I	CTGAAG	6 cut(s)	819, 2094, 2538, 2897, 3324, 3363
Eco72I	CACGTG	2 cut(s)	2936, 3041
Eco88I	CYCGRG	2 cut(s)	245, 1623
EcoNI	CCTNNNNNAGG	1 cut(s)	696
EcoO109I	RGGNCCY	3 cut(s)	1240, 1767, 2067
EcoRI	GAATTC	1 cut(s)	502
EcoRII	CCWGG	4 cut(s)	416, 985, 1243, 1642
EcoT14I	CCWWGG	2 cut(s)	854, 1662
EcoT38I	GRGCYC	2 cut(s)	1277, 3294
ErhI	CCWWGG	2 cut(s)	854, 1662
FaqI	GGGAC	3 cut(s)	1145, 1717, 1883
FauI	CCCGC	1 cut(s)	624
FbaI	TGATCA	1 cut(s)	904
FblI	GTMKAC	1 cut(s)	941
FriOI	GRGCYC	2 cut(s)	1277, 3294
GlaI	GCGC	4 cut(s)	2814, 3136, 3228, 3357
GsaI	CCCAGC	2 cut(s)	1925, 2907
GsuI	CTGGAG	2 cut(s)	702, 1503
HaeII	RGCGCY	1 cut(s)	3138
HaeIII	GGCC	6 cut(s)	712, 928, 1917, 2068, 2766, 2787
HapII	CCGG	4 cut(s)	174, 734, 1828, 3359
HgaI	GACGC	1 cut(s)	2049
HhaI	GCGC	4 cut(s)	2815, 3137, 3229, 3358
Hin6I	GCGC	4 cut(s)	2813, 3135, 3227, 3356
HinP1I	GCGC	4 cut(s)	2813, 3135, 3227, 3356
HincII	GTYRAC	3 cut(s)	964, 1004, 1258
HindII	GTYRAC	3 cut(s)	964, 1004, 1258
HindIII	AAGCTT	2 cut(s)	532, 2756
HpaI	GTTAAC	2 cut(s)	964, 1258
HpaII	CCGG	4 cut(s)	174, 734, 1828, 3359
HphI	GGTGA	5 cut(s)	633, 1355, 1730, 1856, 2445
HpyAV	CCTTC	8 cut(s)	780, 794, 885, 1101, 1499, 1745, 3180, 3299
HpyCH4IV	ACGT	5 cut(s)	1350, 2935, 3040, 3204, 3282
HpyF3I	CTNAG	8 cut(s)	11, 348, 1158, 1199, 2200, 2838, 3146, 3396
HpySE526I	ACGT	5 cut(s)	1350, 2935, 3040, 3204, 3282
HspAI	GCGC	4 cut(s)	2813, 3135, 3227, 3356
KpnI	GGTACC	1 cut(s)	1122
KroI	GCCGGC	1 cut(s)	3358
KroNI	GCCGGC	1 cut(s)	3360
Ksp22I	TGATCA	1 cut(s)	904
KspAI	GTTAAC	2 cut(s)	964, 1258
Kzo9I	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
LmnI	GCTCC	6 cut(s)	1162, 1272, 1522, 1645, 2828, 3328
MaeII	ACGT	5 cut(s)	1350, 2935, 3040, 3204, 3282
MaeIII	GTNAC	6 cut(s)	778, 976, 1039, 2080, 2320, 2423
MalI	GATC	9 cut(s)	35, 267, 603, 667, 906, 1156, 1197, 1621, 3390
MboI	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
MboII	GAAGA	4 cut(s)	338, 2240, 2531, 3173
MfeI	CAATTG	1 cut(s)	145
MflI	RGATCY	4 cut(s)	665, 1154, 1195, 1619
MhlI	GDGCHC	8 cut(s)	637, 706, 993, 1211, 1277, 1658, 2979, 3294
MlyI	GAGTC	3 cut(s)	853, 1730, 2977
MmeI	TCCRAC	4 cut(s)	737, 890, 1132, 3352
MroNI	GCCGGC	1 cut(s)	3358
MroXI	GAANNNNTTC	3 cut(s)	1554, 2526, 2587
MspA1I	CMGCKG	4 cut(s)	839, 2123, 2165, 2903
MspCI	CTTAAG	2 cut(s)	440, 497
MspI	CCGG	4 cut(s)	174, 734, 1828, 3359
MspR9I	CCNGG	6 cut(s)	175, 418, 734, 987, 1245, 1644
MunI	CAATTG	1 cut(s)	145
Mva1269I	GAATGC	4 cut(s)	1018, 1403, 2022, 2815
MvaI	CCWGG	4 cut(s)	418, 987, 1245, 1644
MvnI	CGCG	2 cut(s)	631, 2736
NaeI	GCCGGC	1 cut(s)	3360
NciI	CCSGG	2 cut(s)	175, 734
NcoI	CCATGG	1 cut(s)	1662
NdeII	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
NgoMIV	GCCGGC	1 cut(s)	3358
NlaIV	GGNNCC	9 cut(s)	636, 667, 738, 1120, 1242, 1274, 1518, 3036, 3324
NmuCI	GTSAC	2 cut(s)	1039, 2080
NspI	RCATGY	2 cut(s)	970, 1382
OliI	CACNNNNGTG	2 cut(s)	185, 609
PaeR7I	CTCGAG	2 cut(s)	245, 1623
PagI	TCATGA	1 cut(s)	1510
PaqCI	CACCTGC	2 cut(s)	652, 2653
PceI	AGGCCT	1 cut(s)	1917
PciI	ACATGT	2 cut(s)	966, 1378
PctI	GAATGC	4 cut(s)	1018, 1403, 2022, 2815
PdiI	GCCGGC	1 cut(s)	3360
PdmI	GAANNNNTTC	3 cut(s)	1554, 2526, 2587
Pfl23II	CGTACG	1 cut(s)	1351
PflMI	CCANNNNNTGG	2 cut(s)	662, 680
PinAI	ACCGGT	1 cut(s)	1827
PleI	GAGTC	3 cut(s)	853, 1729, 2977
PmaCI	CACGTG	2 cut(s)	2936, 3041
PmlI	CACGTG	2 cut(s)	2936, 3041
PpsI	GAGTC	3 cut(s)	853, 1729, 2977
Ppu21I	YACGTR	2 cut(s)	2936, 3041
PpuMI	RGGWCCY	2 cut(s)	1240, 1767
PscI	ACATGT	2 cut(s)	966, 1378
PshAI	GACNNNNGTC	1 cut(s)	2511
PshBI	ATTAAT	1 cut(s)	3165
PsiI	TTATAA	1 cut(s)	1545
Psp5II	RGGWCCY	2 cut(s)	1240, 1767
Psp6I	CCWGG	4 cut(s)	416, 985, 1243, 1642
PspCI	CACGTG	2 cut(s)	2936, 3041
PspFI	CCCAGC	2 cut(s)	1921, 2903
PspGI	CCWGG	4 cut(s)	416, 985, 1243, 1642
PspLI	CGTACG	1 cut(s)	1351
PspN4I	GGNNCC	9 cut(s)	636, 667, 738, 1120, 1242, 1274, 1518, 3036, 3324
PspPI	GGNCC	6 cut(s)	270, 710, 824, 1240, 1767, 2067
PspPPI	RGGWCCY	2 cut(s)	1240, 1767
PstI	CTGCAG	2 cut(s)	1224, 2164
PstNI	CAGNNNCTG	4 cut(s)	797, 2123, 2168, 2576
PsuI	RGATCY	4 cut(s)	665, 1154, 1195, 1619
PvuII	CAGCTG	4 cut(s)	839, 2123, 2165, 2903
RsaI	GTAC	8 cut(s)	167, 628, 1120, 1353, 1684, 2805, 2940, 3207
RsaNI	GTAC	8 cut(s)	166, 627, 1119, 1352, 1683, 2804, 2939, 3206
Sau3AI	GATC	9 cut(s)	33, 265, 601, 665, 904, 1154, 1195, 1619, 3388
Sau96I	GGNCC	6 cut(s)	270, 710, 824, 1240, 1767, 2067
SbfI	CCTGCAGG	1 cut(s)	1224
SchI	GAGTC	3 cut(s)	853, 1730, 2977
ScrFI	CCNGG	6 cut(s)	175, 418, 734, 987, 1245, 1644
SdaI	CCTGCAGG	1 cut(s)	1224
SduI	GDGCHC	8 cut(s)	637, 706, 993, 1211, 1277, 1658, 2979, 3294
SfcI	CTRYAG	2 cut(s)	1220, 2160
Sfr274I	CTCGAG	2 cut(s)	245, 1623
SinI	GGWCC	4 cut(s)	270, 824, 1240, 1767
SlaI	CTCGAG	2 cut(s)	245, 1623
SmlI	CTYRAG	9 cut(s)	245, 440, 497, 1306, 1623, 2296, 2473, 2673, 3366
SmoI	CTYRAG	9 cut(s)	245, 440, 497, 1306, 1623, 2296, 2473, 2673, 3366
SpeI	ACTAGT	1 cut(s)	1168
Sse8387I	CCTGCAGG	1 cut(s)	1224
SseBI	AGGCCT	1 cut(s)	1917
SsiI	CCGC	8 cut(s)	199, 631, 1939, 2020, 2225, 2734, 3154, 3224
SspI	AATATT	2 cut(s)	1882, 2686
StuI	AGGCCT	1 cut(s)	1917
StyD4I	CCNGG	6 cut(s)	173, 416, 732, 985, 1243, 1642
StyI	CCWWGG	2 cut(s)	854, 1662
TaiI	ACGT	5 cut(s)	1353, 2938, 3043, 3207, 3285
TaqI	TCGA	7 cut(s)	48, 222, 246, 506, 1624, 1812, 2333
TaqII	GACCGA	1 cut(s)	258
TatI	WGTACW	2 cut(s)	2803, 2938
TauI	GCSGC	1 cut(s)	3227
TscAI	CASTG	8 cut(s)	663, 781, 1050, 1584, 2269, 2928, 3035, 3358
TseFI	GTSAC	2 cut(s)	1039, 2080
Tsp45I	GTSAC	2 cut(s)	1039, 2080
TspGWI	ACGGA	3 cut(s)	461, 529, 879
TspRI	CASTG	8 cut(s)	663, 781, 1050, 1584, 2269, 2928, 3035, 3358
Van91I	CCANNNNNTGG	2 cut(s)	662, 680
Vha464I	CTTAAG	2 cut(s)	440, 497
VneI	GTGCAC	3 cut(s)	702, 1654, 2975
VpaK11BI	GGWCC	4 cut(s)	270, 824, 1240, 1767
VspI	ATTAAT	1 cut(s)	3165
XagI	CCTNNNNNAGG	1 cut(s)	696
XapI	RAATTY	3 cut(s)	59, 502, 1862
XceI	RCATGY	2 cut(s)	970, 1382
XhoI	CTCGAG	2 cut(s)	245, 1623
XmiI	GTMKAC	1 cut(s)	941
XmnI	GAANNNNTTC	3 cut(s)	1554, 2526, 2587

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.