Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Forward (+)

59140621 .. 59147692

Gene Structure 7072 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ26695

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3276 bp

ATGGCGACGCCATCGGCGACAACTACAACACTGACTAAAGACCTCACCTTCTTGATCATGCAATTCCTTGACGAGGACAAGTGGAAGGATACGCTTCATAGGCTTCAGCAAGAATCAGGATACTTCTTCGACATGAAGTATTTCGAGGAATTATTGCTCGAGGGAAATTGGAATGAAGTCGAGAAGTATCTTTCCGGGTTCACTAAAGTGGATGACAACCGCTATTCAATGAAGATATTCTTCGAAATTCGTAAGCAGAAATACTTAGAAGCGCTGGATAGACATGATAGAGCTGCCGCAGTAGATATCCTAGTGAAGGATCTGAAAGTCTTTGCAGGTTTTAACGAAGACTTGTACAAGGAAATCACTCAGCTGCTCACTTTGAACAATTTTAGAGACAATGCTCAACTCTCTGCTTATGCAGATATAAGGACTGCAAGAGCAATTATGGCCCAAGAACTTAAGAAGCTTGTTGAGGCCAACCCTTCTTTCCGTGAAAAATTACAGTTCCCTAGCATTAAAAGTTCAAGGCTACGGACGCTCATTAACCAAAGCTTAAATTGGCAGCATTCCCTTTGTCCTAATCCTAAACAAAATCCGGAAATAAAAACCCTATTTGTGGATCACACTTGTAGACATTCCAACGATCCATTTGCTCATTTAGCAGCAAATGGTCAGGCTCAACAGATGGGAGCAGTACAGAGAGCTGATGGCTTCATTCCTATCGGTGTAAATGGGTCCTTCCAACCCCCAATAGCACCAGTACCAATTCCACCTCCTTTGTCAACATGGACGTCCATACCCCCCAGTGTCAATCAGTTGGCAGCTTCTGCAGGAGGTATTGGTTTTGGCAGTCCAGCAAATCCAGCTTCATATTTGAACAGTCCAGCAAATCCTGAAGATTTGTTCAGAACACGGTTCCCTGGGGTTTCAGACAGGGTTATACTGCCAGGGGTTAATCCAGTCCACGTTCCAAGTCCAGCATTCAATGCAATTGATGAGTTTCCGAAGGCTGTTGCCCGGATACTGAATCAGGGCTCAGTTCCCACTAGCATGGACTTCCATCCTCTTCAACAGACTATACTTCTAGTTGGAACTAGTATGGGGGATATAAGTGTCTGGGAAGTCAGTTCTAGGGAGAAGTTGGTATCAAGAAGCTTTCAGCCGTGGGATATGGGGGCAAGTTCAATGATACTAAAGGCAACTTTAGTCAAGGATCCAAGTGTCTCAGTTAAGCGTGTACTATGGAGCCCTGATGGTTCTCTATTCGGAGTTGCATATTCTAAACACATGATGCAGTTATATGGTTATTTTGGTGGTGATGATGTTCGTCAGCATTTAGAGATCGATGCTCATGTTGGTAGTGTAAATGATCTGGCATTCTGCAACCCAACGAAGCAACTCTGTGTCATAACTTGCGGTGATGATAAGGCCATCAAGGTCTGGGATGCGGCTACTGGATCAAAATTGTATACTTTTGAAGGTCATGAGGCTCCTGTTCATTCTGTGTGCCCTCATAGCAAAGAACATGTCCATTTTGTCTTTTCGACATCAGTAGATGGACAAATAAAAGCATGGTTATATGAAGTGGTGGGGTCCAGGGTTAATTACGATGCTCCCAGCCTCTCATGCACAACGATGGTTTATAGTGCAGATGGTAAAAGGCTTTTTTCATGTGGGACGAGCAAAGATGGAGAATCTCATGTTGTTGAATGGAATGAAAATGAAGGCACAATAAAGAGGAATTACCAAGGATTTCAAAAACAATCTTTGGGTGTTGTTCAATTTGACACAACCAGGAATAAGTATCTAGCAGTTGGTGACGACTATGCAATCAAAGTTTGGGATATGGATAATATCAATCTTTTGGCTACTATTGATGCTGAAGGAGGTCTACTAGCAACCCCCCGTATCCGCTTCAACAAGGAAGGATCTTTGTTAGCTGTTTCTGCAAATGACAACAGAATCAAAGTTTTGGCTACAACAGATGGTCTCCGATTGATGCGCAATTATGAAAGTCTCTCTCTTATTGCCTCCAGAAATGCACCAGAAAAGAATGGTAGCACCAGAAGCATGGAGGATAATAAGAAACCGAAGTTAACTGAAGAGCTCAAACCTGCAAAGATCTGGAAGCCCACTGAGATTAGTGAAACTGCTCGGCTTCGGTCTTTAAGGCTTTCGACAACTACAGTTAAAACAGACAAGATCTCAAGGTTGGTTTACACTAATTCAGGTAATGCCATTCTGGCATTATCATCAAATGGAACCCATCTACTATGGAGGTGGCCACAAGCTGACCGTAGTACGATTGGCAAGGCAACAACCAGTGTTACCCCAAAATTCGTGGAACAAGCTTCAGGCATCCTGATGATCAATGATCTTACTGGTGCTAAACCTGAAGATGCAATACCTTGCTTTGCTTTGTCCAAGAATGATTCGTATGTCATGTCAGCCTCTGGAGGGAAGATATCGTTGTTCAACCTAATGACATTCAAGACGATGACAACTTTTATGTCGCCACCACCTGTGGTAACATGTCTTGCTTTCCACCCTGAAGACAATAATATAGTTGCTGTTGGATTTGATGATTCAACTATTCACATATACAATGTCCGTATCGATGAGGTTAGGATAAAGCTCAAGGGTCACTCTAAGAGAGTCACAGGCCTTGTGTTCTCTCAACTTCTGAAGACACTTGTTTCTTCTGGAGCAGATGCTCAGATAGTTGTGTGGAGCTCTGATAAGTGGGAAAGGCAGAAGAACAGTTTCTTGCAGGTTCCAGCTTGGACAACACCCACAGCACTGTTTGGAACTCATCTTCAGTATCAGAAGGATCAGAATCACTTCCTCGTTGTACACGAGACTCAACTTGCTGTATACGAAACCTTGAGGCTTGAATGCCAGAAGAAGTGGGTAGTAGGAGAATCTTCAGCACCAATCTCCTATGCAACATTCTCTTGTGATAGCCAGTTAGTCTACGCGAGCTTTCTTGATGGAACTGTGTGCGTGTTTGCAGCATCGAATCTTCAAATGCAATGCCAGATTAACCCCTATGCTTATCTTCCACCTAATGTCAGCTCTGCAACATACCCCCTAGTGATAGCTGCGAATCCACAAGAGCCGAACCAGTTTGCAGTGGGACTAACAGATGGTGCAGTTGTCATGTTTGAACCACTTGAGTCTGGAGACAAATGGGGTGTGCTGCCACCAGCAGACAATGGTTGCTTCCATGCTTCAACTTCTGCCAATGGTTCCAGTTTAGAACTCCAGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1091

Amino Acids

120.84

Weight (kDa)

6.51

Isoelectric Point (pI)

36.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	13 - 40	1.5e-08	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	44 - 168	1.1e-58	TPR1-like, CTLH-containing domain
zf_topless	PF21359	171 - 211	2.4e-24	TOPLESS, zinc finger domain
WDR55	PF24796	348 - 658	1.5e-10	WDR55
Beta-prop_WDR3_1st	PF25173	390 - 561	3.4e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	408 - 560	2.2e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	410 - 667	3.7e-24	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	410 - 561	2.7e-14	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	417 - 526	3.6e-09	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	426 - 528	8.2e-10	WDHD1 first WD40 domain
Beta-prop_EML	PF23409	448 - 626	3.3e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	449 - 659	3e-16	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	461 - 528	6.6e-07	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	462 - 621	7e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	470 - 561	8.5e-06	WDR90/POC16, second beta-propeller
Beta-prop_WDR5	PF25175	512 - 665	3.8e-09	WDR5 beta-propeller domain
WD40_Prp19	PF24814	727 - 998	9.2e-15	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	798 - 918	3.1e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	800 - 915	1.5e-14	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	800 - 940	3e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	801 - 1006	1.3e-17	WDR5 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	802 - 1034	9.9e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	805 - 1011	5.1e-12	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	808 - 1008	2.4e-18	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	809 - 912	9.3e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	814 - 912	2.4e-16	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	816 - 912	8.8e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	816 - 924	5.3e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	821 - 915	2.8e-09	WDR90/POC16, second beta-propeller
Beta-prop_THOC3	PF25174	858 - 1015	4.1e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	2006
Acc36I	ACCTGC	3 cut(s)	326, 2125, 2764
AccI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
AccII	CGCG	1 cut(s)	2981
AccIII	TCCGGA	1 cut(s)	598
AciI	CCGC	5 cut(s)	220, 297, 1419, 1451, 1915
AclWI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
AcoI	YGGCCR	1 cut(s)	2285
AcsI	RAATTY	2 cut(s)	246, 2339
AcyI	GRCGYC	2 cut(s)	8, 794
AfaI	GTAC	6 cut(s)	356, 699, 765, 1242, 2305, 2856
AfeI	AGCGCT	1 cut(s)	273
AfiI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
AflII	CTTAAG	1 cut(s)	461
AflIII	ACRYGT	2 cut(s)	1528, 2534
AhlI	ACTAGT	1 cut(s)	1097
AjnI	CCWGG	4 cut(s)	922, 949, 1598, 1796
AjuI	GAANNNNNNNTTGG	2 cut(s)	473, 505
AleI	CACNNNNGTG	1 cut(s)	206
Alw21I	GWGCWC	2 cut(s)	2112, 2738
Alw26I	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 2855, 3180
AlwI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
AlwNI	CAGNNNCTG	2 cut(s)	830, 2456
Ama87I	CYCGRG	1 cut(s)	158
Aor13HI	TCCGGA	1 cut(s)	598
Aor51HI	AGCGCT	1 cut(s)	273
AoxI	GGCC	5 cut(s)	450, 477, 1431, 2285, 2665
ApeKI	GCWGC	8 cut(s)	293, 373, 565, 665, 824, 3014, 3104, 3202
ApoI	RAATTY	2 cut(s)	246, 2339
ArsI	GACNNNNNNTTYG	2 cut(s)	1515, 1547
Asp700I	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
AspLEI	GCGC	2 cut(s)	274, 2007
AspS9I	GGNCC	3 cut(s)	451, 738, 1596
AsuC2I	CCSGG	2 cut(s)	196, 1021
AsuHPI	GGTGA	4 cut(s)	37, 1331, 1433, 1832
AsuII	TTCGAA	1 cut(s)	243
AvaI	CYCGRG	1 cut(s)	158
AvaII	GGWCC	2 cut(s)	738, 1596
BaeGI	GKGCMC	1 cut(s)	1514
BalI	TGGCCA	1 cut(s)	2287
BamHI	GGATCC	1 cut(s)	1216
BanII	GRGCYC	4 cut(s)	1040, 1253, 2112, 2738
BarI	GAAGNNNNNNTAC	4 cut(s)	1878, 1910, 2391, 2423
BauI	CACGAG	1 cut(s)	2858
BbsI	GAAGAC	3 cut(s)	354, 2562, 2696
Bbv12I	GWGCWC	2 cut(s)	2112, 2738
BbvI	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 3026, 3091, 3189
BceAI	ACGGC	1 cut(s)	1150
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	4 cut(s)	924, 951, 1600, 1798
BciVI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BclI	TGATCA	2 cut(s)	54, 2370
BcnI	CCSGG	2 cut(s)	196, 1021
BcoDI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 2855, 3180
BcuI	ACTAGT	1 cut(s)	1097
BfaI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 1898, 3095
BfmI	CTRYAG	2 cut(s)	831, 2187
BfoI	RGCGCY	1 cut(s)	275
BfrI	CTTAAG	1 cut(s)	461
BfuAI	ACCTGC	3 cut(s)	326, 2125, 2764
BfuI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BglI	GCCNNNNNGGC	1 cut(s)	2246
BglII	AGATCT	2 cut(s)	2124, 2205
Bme1390I	CCNGG	6 cut(s)	196, 924, 951, 1021, 1600, 1798
Bme18I	GGWCC	2 cut(s)	738, 1596
BmeT110I	CYCGRG	1 cut(s)	158
BmgT120I	GGNCC	3 cut(s)	451, 738, 1596
BmiI	GGNNCC	9 cut(s)	739, 920, 1218, 1250, 1494, 1597, 2266, 2778, 3253
BmrFI	CCNGG	6 cut(s)	196, 924, 951, 1021, 1600, 1798
BmrI	ACTGGG	1 cut(s)	801
BmuI	ACTGGG	1 cut(s)	801
BpiI	GAAGAC	3 cut(s)	354, 2562, 2696
BpmI	CTGGAG	5 cut(s)	2020, 2478, 2727, 3204, 3251
Bpu14I	TTCGAA	1 cut(s)	243
BpuEI	CTTGAG	4 cut(s)	2194, 2624, 2908, 3197
BpuMI	CCSGG	2 cut(s)	196, 1021
Bsa29I	ATCGAT	2 cut(s)	1347, 2619
BsaBI	GATNNNNATC	1 cut(s)	2838
BsaHI	GRCGYC	2 cut(s)	8, 794
BsaI	GGTCTC	1 cut(s)	1997
BsaJI	CCNNGG	6 cut(s)	922, 923, 950, 1166, 1599, 1750
BsaWI	WCCGGW	1 cut(s)	598
Bsc4I	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
Bse1I	ACTGG	9 cut(s)	761, 807, 962, 1462, 2325, 2389, 2968, 3127, 3255
Bse3DI	GCAATG	1 cut(s)	3041
Bse8I	GATNNNNATC	1 cut(s)	2838
BseAI	TCCGGA	1 cut(s)	598
BseBI	CCWGG	4 cut(s)	924, 951, 1600, 1798
BseCI	ATCGAT	2 cut(s)	1347, 2619
BseDI	CCNNGG	6 cut(s)	922, 923, 950, 1166, 1599, 1750
BseGI	GGATG	4 cut(s)	217, 1063, 1453, 2361
BseJI	GATNNNNATC	1 cut(s)	2838
BseLI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
BseMI	GCAATG	1 cut(s)	3041
BseMII	CTCAG	5 cut(s)	383, 1053, 1242, 2130, 2732
BseNI	ACTGG	9 cut(s)	761, 807, 962, 1462, 2325, 2389, 2968, 3127, 3255
BseSI	GKGCMC	1 cut(s)	1514
BseXI	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 3026, 3091, 3189
BseYI	CCCAGC	1 cut(s)	1619
BsgI	GTGCAG	2 cut(s)	1671, 3174
Bsh1236I	CGCG	1 cut(s)	2981
BshFI	GGCC	5 cut(s)	452, 479, 1433, 2287, 2667
BshVI	ATCGAT	2 cut(s)	1347, 2619
BsiHKAI	GWGCWC	2 cut(s)	2112, 2738
BsiHKCI	CYCGRG	1 cut(s)	158
BsiSI	CCGG	3 cut(s)	195, 599, 1021
BslFI	GGGAC	2 cut(s)	1693, 3153
BslI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2460
BsmAI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 2855, 3180
BsmFI	GGGAC	2 cut(s)	1693, 3153
BsmI	GAATGC	4 cut(s)	568, 983, 1379, 2903
BsnI	GGCC	5 cut(s)	452, 479, 1433, 2287, 2667
Bso31I	GGTCTC	1 cut(s)	1997
BsoBI	CYCGRG	1 cut(s)	158
Bsp119I	TTCGAA	1 cut(s)	243
Bsp1286I	GDGCHC	5 cut(s)	1040, 1253, 1514, 2112, 2738
Bsp13I	TCCGGA	1 cut(s)	598
Bsp1407I	TGTACA	2 cut(s)	354, 2854
BspACI	CCGC	5 cut(s)	220, 297, 1419, 1451, 1915
BspANI	GGCC	5 cut(s)	452, 479, 1433, 2287, 2667
BspCNI	CTCAG	5 cut(s)	382, 1052, 1241, 2131, 2731
BspDI	ATCGAT	2 cut(s)	1347, 2619
BspEI	TCCGGA	1 cut(s)	598
BspFNI	CGCG	1 cut(s)	2981
BspHI	TCATGA	1 cut(s)	1486
BspLI	GGNNCC	9 cut(s)	739, 920, 1218, 1250, 1494, 1597, 2266, 2778, 3253
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	3 cut(s)	326, 2125, 2764
BspPI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1939, 2841
BspQI	GCTCTTC	1 cut(s)	2100
BspT104I	TTCGAA	1 cut(s)	243
BspTI	CTTAAG	1 cut(s)	461
BspTNI	GGTCTC	1 cut(s)	1997
BsrDI	GCAATG	1 cut(s)	3041
BsrGI	TGTACA	2 cut(s)	354, 2854
BsrI	ACTGG	9 cut(s)	761, 807, 962, 1462, 2325, 2389, 2968, 3127, 3255
BssECI	CCNNGG	6 cut(s)	922, 923, 950, 1166, 1599, 1750
BssNAI	GTATAC	2 cut(s)	1473, 2878
BssNI	GRCGYC	2 cut(s)	8, 794
BssSI	CACGAG	1 cut(s)	2858
BssT1I	CCWWGG	1 cut(s)	1750
Bst1107I	GTATAC	2 cut(s)	1473, 2878
Bst2BI	CACGAG	1 cut(s)	2858
Bst2UI	CCWGG	4 cut(s)	924, 951, 1600, 1798
Bst4CI	ACNGT	8 cut(s)	507, 884, 918, 2191, 2300, 2765, 2805, 3001
Bst6I	CTCTTC	2 cut(s)	1074, 2100
BstACI	GRCGYC	2 cut(s)	8, 794
BstAFI	CTTAAG	1 cut(s)	461
BstAPI	GCANNNNNTGC	2 cut(s)	830, 989
BstAUI	TGTACA	2 cut(s)	354, 2854
BstBI	TTCGAA	1 cut(s)	243
BstC8I	GCNNGC	1 cut(s)	2983
BstDEI	CTNAG	7 cut(s)	265, 369, 1039, 1228, 2139, 2652, 2718
BstDSI	CCRYGG	1 cut(s)	1166
BstENI	CCTNNNNNAGG	2 cut(s)	71, 314
BstF5I	GGATG	4 cut(s)	217, 1063, 1453, 2361
BstFNI	CGCG	1 cut(s)	2981
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	2 cut(s)	274, 2007
BstMAI	GTCTC	6 cut(s)	390, 1231, 1997, 2024, 2855, 3180
BstNI	CCWGG	4 cut(s)	924, 951, 1600, 1798
BstNSI	RCATGY	2 cut(s)	1532, 2538
BstSCI	CCNGG	6 cut(s)	194, 922, 949, 1019, 1598, 1796
BstSFI	CTRYAG	2 cut(s)	831, 2187
BstSLI	GKGCMC	1 cut(s)	1514
BstUI	CGCG	1 cut(s)	2981
BstV1I	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 3026, 3091, 3189
BstV2I	GAAGAC	3 cut(s)	354, 2562, 2696
BstX2I	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
BstXI	CCANNNNNNTGG	2 cut(s)	1054, 2074
BstYI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
BstZ17I	GTATAC	2 cut(s)	1473, 2878
Bsu15I	ATCGAT	2 cut(s)	1347, 2619
BsuI	GTATCC	4 cut(s)	82, 113, 1017, 1922
BsuRI	GGCC	5 cut(s)	452, 479, 1433, 2287, 2667
BsuTUI	ATCGAT	2 cut(s)	1347, 2619
BtgI	CCRYGG	1 cut(s)	1166
BtsCI	GGATG	4 cut(s)	217, 1063, 1453, 2361
BtsI	GCAGTG	1 cut(s)	3141
BtsIMutI	CAGTG	6 cut(s)	29, 814, 2136, 2332, 2801, 3141
BveI	ACCTGC	3 cut(s)	326, 2125, 2764
Cac8I	GCNNGC	1 cut(s)	2983
CaiI	CAGNNNCTG	2 cut(s)	830, 2456
CciI	TCATGA	1 cut(s)	1486
CfoI	GCGC	2 cut(s)	274, 2007
Cfr13I	GGNCC	3 cut(s)	451, 738, 1596
ClaI	ATCGAT	2 cut(s)	1347, 2619
CseI	GACGC	2 cut(s)	16, 547
Csp6I	GTAC	6 cut(s)	355, 698, 764, 1241, 2304, 2855
CspCI	CAANNNNNGTGG	4 cut(s)	762, 797, 2325, 2360
CviQI	GTAC	6 cut(s)	355, 698, 764, 1241, 2304, 2855
DdeI	CTNAG	7 cut(s)	265, 369, 1039, 1228, 2139, 2652, 2718
EaeI	YGGCCR	1 cut(s)	2285
Eam1104I	CTCTTC	2 cut(s)	1074, 2100
EarI	CTCTTC	2 cut(s)	1074, 2100
Ecl136II	GAGCTC	2 cut(s)	2110, 2736
Eco130I	CCWWGG	1 cut(s)	1750
Eco147I	AGGCCT	1 cut(s)	2667
Eco24I	GRGCYC	4 cut(s)	1040, 1253, 2112, 2738
Eco31I	GGTCTC	1 cut(s)	1997
Eco32I	GATATC	2 cut(s)	307, 2469
Eco47I	GGWCC	2 cut(s)	738, 1596
Eco47III	AGCGCT	1 cut(s)	273
Eco53kI	GAGCTC	2 cut(s)	2110, 2736
Eco88I	CYCGRG	1 cut(s)	158
EcoICRI	GAGCTC	2 cut(s)	2110, 2736
EcoNI	CCTNNNNNAGG	2 cut(s)	71, 314
EcoO109I	RGGNCCY	1 cut(s)	738
EcoRII	CCWGG	4 cut(s)	922, 949, 1598, 1796
EcoRV	GATATC	2 cut(s)	307, 2469
EcoT14I	CCWWGG	1 cut(s)	1750
EcoT38I	GRGCYC	4 cut(s)	1040, 1253, 2112, 2738
ErhI	CCWWGG	1 cut(s)	1750
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	2 cut(s)	1693, 3153
FbaI	TGATCA	2 cut(s)	54, 2370
FblI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
FokI	GGATG	4 cut(s)	224, 1050, 1460, 2348
FriOI	GRGCYC	4 cut(s)	1040, 1253, 2112, 2738
FspBI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 1898, 3095
FspI	TGCGCA	1 cut(s)	2006
GlaI	GCGC	2 cut(s)	273, 2006
GsaI	CCCAGC	1 cut(s)	1623
GsuI	CTGGAG	5 cut(s)	2020, 2478, 2727, 3204, 3251
HaeII	RGCGCY	1 cut(s)	275
HaeIII	GGCC	5 cut(s)	452, 479, 1433, 2287, 2667
HapII	CCGG	3 cut(s)	195, 599, 1021
HgaI	GACGC	2 cut(s)	16, 547
HhaI	GCGC	2 cut(s)	274, 2007
Hin1I	GRCGYC	2 cut(s)	8, 794
Hin6I	GCGC	2 cut(s)	272, 2005
HinP1I	GCGC	2 cut(s)	272, 2005
HincII	GTYRAC	2 cut(s)	786, 2100
HindII	GTYRAC	2 cut(s)	786, 2100
HindIII	AAGCTT	4 cut(s)	467, 553, 1156, 2352
HpaI	GTTAAC	1 cut(s)	2100
HpaII	CCGG	3 cut(s)	195, 599, 1021
HphI	GGTGA	4 cut(s)	37, 1331, 1433, 1832
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	8 cut(s)	507, 884, 918, 2191, 2300, 2765, 2805, 3001
HpyCH4IV	ACGT	2 cut(s)	794, 969
HpyF3I	CTNAG	7 cut(s)	265, 369, 1039, 1228, 2139, 2652, 2718
HpySE526I	ACGT	2 cut(s)	794, 969
Hsp92I	GRCGYC	2 cut(s)	8, 794
HspAI	GCGC	2 cut(s)	272, 2005
Kpn2I	TCCGGA	1 cut(s)	598
Ksp22I	TGATCA	2 cut(s)	54, 2370
KspAI	GTTAAC	1 cut(s)	2100
LguI	GCTCTTC	1 cut(s)	2100
LmnI	GCTCC	6 cut(s)	692, 1248, 1498, 1621, 2708, 2733
Lsp1109I	GCAGC	8 cut(s)	280, 360, 577, 677, 836, 3026, 3091, 3189
MaeI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 1898, 3095
MaeII	ACGT	2 cut(s)	794, 969
MaeIII	GTNAC	5 cut(s)	1820, 2329, 2530, 2645, 2659
MfeI	CAATTG	1 cut(s)	993
MflI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
MhlI	GDGCHC	5 cut(s)	1040, 1253, 1514, 2112, 2738
MlsI	TGGCCA	1 cut(s)	2287
MluNI	TGGCCA	1 cut(s)	2287
MlyI	GAGTC	3 cut(s)	2667, 2857, 3188
MmeI	TCCRAC	4 cut(s)	666, 769, 1072, 2557
Mox20I	TGGCCA	1 cut(s)	2287
MroI	TCCGGA	1 cut(s)	598
MroXI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
MscI	TGGCCA	1 cut(s)	2287
MslI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
Msp20I	TGGCCA	1 cut(s)	2287
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	3 cut(s)	195, 599, 1021
MspR9I	CCNGG	6 cut(s)	196, 924, 951, 1021, 1600, 1798
MunI	CAATTG	1 cut(s)	993
Mva1269I	GAATGC	4 cut(s)	568, 983, 1379, 2903
MvaI	CCWGG	4 cut(s)	924, 951, 1600, 1798
MvnI	CGCG	1 cut(s)	2981
NciI	CCSGG	2 cut(s)	196, 1021
NlaIV	GGNNCC	9 cut(s)	739, 920, 1218, 1250, 1494, 1597, 2266, 2778, 3253
NmeAIII	GCCGAG	1 cut(s)	2137
NmuCI	GTSAC	3 cut(s)	1820, 2645, 2659
NsbI	TGCGCA	1 cut(s)	2006
NspI	RCATGY	2 cut(s)	1532, 2538
NspV	TTCGAA	1 cut(s)	243
OliI	CACNNNNGTG	1 cut(s)	206
PaeR7I	CTCGAG	1 cut(s)	158
PagI	TCATGA	1 cut(s)	1486
PasI	CCCWGGG	1 cut(s)	923
PceI	AGGCCT	1 cut(s)	2667
PciI	ACATGT	2 cut(s)	1528, 2534
PciSI	GCTCTTC	1 cut(s)	2100
PcsI	WCGNNNNNNNCGW	2 cut(s)	14, 2856
PctI	GAATGC	4 cut(s)	568, 983, 1379, 2903
PdmI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
PleI	GAGTC	3 cut(s)	2666, 2857, 3187
PpsI	GAGTC	3 cut(s)	2666, 2857, 3187
PpuMI	RGGWCCY	1 cut(s)	738
PscI	ACATGT	2 cut(s)	1528, 2534
Psp124BI	GAGCTC	2 cut(s)	2112, 2738
Psp5II	RGGWCCY	1 cut(s)	738
Psp6I	CCWGG	4 cut(s)	922, 949, 1598, 1796
PspFI	CCCAGC	1 cut(s)	1619
PspGI	CCWGG	4 cut(s)	922, 949, 1598, 1796
PspN4I	GGNNCC	9 cut(s)	739, 920, 1218, 1250, 1494, 1597, 2266, 2778, 3253
PspPI	GGNCC	3 cut(s)	451, 738, 1596
PspPPI	RGGWCCY	1 cut(s)	738
PspXI	VCTCGAGB	1 cut(s)	158
PstI	CTGCAG	1 cut(s)	835
PstNI	CAGNNNCTG	2 cut(s)	830, 2456
PsuI	RGATCY	5 cut(s)	319, 1216, 1931, 2124, 2205
PvuII	CAGCTG	1 cut(s)	373
RsaI	GTAC	6 cut(s)	356, 699, 765, 1242, 2305, 2856
RsaNI	GTAC	6 cut(s)	355, 698, 764, 1241, 2304, 2855
RseI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
SacI	GAGCTC	2 cut(s)	2112, 2738
SapI	GCTCTTC	1 cut(s)	2100
Sau96I	GGNCC	3 cut(s)	451, 738, 1596
SchI	GAGTC	3 cut(s)	2667, 2857, 3188
ScrFI	CCNGG	6 cut(s)	196, 924, 951, 1021, 1600, 1798
SduI	GDGCHC	5 cut(s)	1040, 1253, 1514, 2112, 2738
SfcI	CTRYAG	2 cut(s)	831, 2187
Sfr274I	CTCGAG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	243
SinI	GGWCC	2 cut(s)	738, 1596
SlaI	CTCGAG	1 cut(s)	158
SmiMI	CAYNNNNRTG	4 cut(s)	206, 1052, 1637, 2366
SmlI	CTYRAG	6 cut(s)	158, 461, 2209, 2639, 2887, 3176
SmoI	CTYRAG	6 cut(s)	158, 461, 2209, 2639, 2887, 3176
SpeI	ACTAGT	1 cut(s)	1097
SseBI	AGGCCT	1 cut(s)	2667
SsiI	CCGC	5 cut(s)	220, 297, 1419, 1451, 1915
SspMI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 1898, 3095
SstI	GAGCTC	2 cut(s)	2112, 2738
StuI	AGGCCT	1 cut(s)	2667
StyD4I	CCNGG	6 cut(s)	194, 922, 949, 1019, 1598, 1796
StyI	CCWWGG	1 cut(s)	1750
TaaI	ACNGT	8 cut(s)	507, 884, 918, 2191, 2300, 2765, 2805, 3001
TaiI	ACGT	2 cut(s)	797, 972
TaqII	GACCGA	1 cut(s)	2154
TatI	WGTACW	4 cut(s)	354, 697, 1240, 2854
TauI	GCSGC	2 cut(s)	299, 1454
TscAI	CASTG	6 cut(s)	36, 814, 2143, 2332, 2808, 3141
TseFI	GTSAC	3 cut(s)	1820, 2645, 2659
TseI	GCWGC	8 cut(s)	293, 373, 565, 665, 824, 3014, 3104, 3202
Tsp45I	GTSAC	3 cut(s)	1820, 2645, 2659
TspGWI	ACGGA	3 cut(s)	482, 550, 2603
TspRI	CASTG	6 cut(s)	36, 814, 2143, 2332, 2808, 3141
Vha464I	CTTAAG	1 cut(s)	461
VpaK11BI	GGWCC	2 cut(s)	738, 1596
XagI	CCTNNNNNAGG	2 cut(s)	71, 314
XapI	RAATTY	2 cut(s)	246, 2339
XceI	RCATGY	2 cut(s)	1532, 2538
XhoI	CTCGAG	1 cut(s)	158
XmiI	GTMKAC	5 cut(s)	634, 1472, 1894, 2877, 2976
XmnI	GAANNNNTTC	4 cut(s)	140, 236, 2765, 2843
XspI	CTAG	9 cut(s)	311, 513, 1050, 1088, 1098, 1134, 1811, 1898, 3095
ZraI	GACGTC	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.