Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Forward (+)

52944778 .. 52952174

Gene Structure 7397 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG375100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3420 bp

ATGTCTTCCCTCAGTAGGGAGCTTGTGTTCCTGATCTTGCAGTTTCTAGATGAGGAAAAATTCAAAGAGACTGTTCACAAGCTAGAACAGGAATCTGGGTTTTTCTTCAATATGAAATATTTTGAGGATGAGGTGCATAATGGCAATTGGGATGAAGTAGAGAAGTACCTCTCTGGGTTCACTAAAGTGGACGATAACCGCTACTCCATGAAAATATTTTTCGAGATAAGGAAGCAGAAGTATCTCGAGGCATTGGACAAGCATGACCGGTCCAAAGCGGTGGACATATTGGTGAAGGATTTAAAGGTTTTTGCCACATTTAATGAAGAACTTTTCAAGGAAATCACTCAGCTTTTGACACTGGATAATTTTAGGGAGAATGAACAGCTATCCAAATATGGAGATACAAAGTCTGCCAGGGCGATTATGCTGGTTGAACTTAAGAAACTGATTGAGGCGAACCCCTTATTCCGTGATAAATTGCAATTCCCAAACCTCAAAAATTCGAGGCTGCGAACTCTGATTAACCAAAGCTTAAATTGGCAGCATCAGCTTTGTAAGAATCCAAGGCCGAATCCAGATATAAAAACTCTTTTTGTGGATCACTCATGCGGACAACCCAATGGTGCTCGGGCCCCGTCACCTGCAAACAATCCATTGCTTGGATCCTTACCAAAAGCTGGAGGATTTCCTCCTCTAGGTGCACATGGGCCCTTTCAACCTACACCAGCCCCGGTTCCAATACCTCTTGCAGGTTGGATGTCTAATGCTTCTACTGTTACTCATCCAGCAGTTTCTGAAGGAGGAGCTATTGGTCTTGGTGGTCCCTCGATAACAGCTGCCTTGAAACATCCAAGGACTCCTCCAACTAACCCTTCTGTAGATTACCCGTCTGGGGATTCTGATCATGTTTCTAAGCGAACAAGACCCATGGGACTTTCCACTGAGGTAAATCTCCCTGTTAACATATTGCCAGTGACATTTCCGGGTCACAGTCACAGTCAGGCGTTGAATGCACCTGATGACTTGCCAAAGAACGTCGCACGAACTTTAAATCAGGGTTCATCGCCCATGAGCATGGATTTTCATCCTGTTCAGCAGACTCTGCTTCTTGTTGGTACCAATGTGGGGGACATAGGGCTGTGGGAAGTTGGATCCAGGGAGCGACTGGTTTTAAGGAACTTCAAAGTTTGGGATGTTAGTACTTGTTCAATGCCTCTACAGGCTGCTCTGGTAAAGGATCCTGGTGTTTCTGTTAACCGTGTGATTTGGAGCCCTGATGGTTCTTTATTTGGTGTTGCTTACTCAAGGCACATTGTTCAAATATATTCTTATCACGGAGGTGATGATATACGACAACACTTAGAGATTGATGCTCATGTTGGTGGAGTAAATGATCTAGCATTCTCCCACCCCAATAAGCAGCTTTGTGTAATAACCTGTGGGGATGACAAGACAATCAAGGTGTGGGATGCTGCGACTGGTTCAAAACAGTATACTTTTGAAGGTCATGAGGCTCCGGTTTATTCTGTCTGCCCACATTATAAGGAAAATATTCAGTTTATCTTTTCAACGGCACTGGATGGAAAAATAAAGGCCTGGCTCTATGACAATTTGGGATCTCGAGTTGATTATGATGCTCCTGGTCGCTGGTGCACAACCATGGCATACAGTGCTGATGGCACAAGGCTCTTTTCATGTGGGACAAGTAAAGATGGTGAGTCATATATTGTTGAATGGAATGAAAGTGAAGGGGCAGTGAAAAGGACCTATCAAGGCTTCCGCAAGCGTTCTTTTGGTGTTGTGCAATTTGATACTACGAAAAACCGGTTTTTGGCTGCTGGTGATGATTTCTCTATTAAATTCTGGGACATGGACAATGTTCAGCTTCTAATGACAGTTGATGCTGATGGAGGCCTCCCGGCAAGCCCCCGTATCCGCTTTAACAAGGATGGCACTCTCTTGGCTGTATCTGCCAATGAGAATGGGATTAAAATTTTGGGCAATGCAGATGGTGTTCGGCTGTTGCACACATTTGAGAATCTTTCTTATGATGCATCAAGGTCCTCTGAAGTTGTGACAAAGCCTGCAATGAACCCAATTTCTGTTGCTGCTGCCGCCGCCGCTGCAGCTGCTACAAGTGCTGGACTGGCTGAGAGAAATGCATCTGCGGTTGCTGTTTCTGGAATGAATGGGGATGCTAGGAACTTGGGGGATGTGAAACCTAGAATAACTGAAGAATCCAATGACAAATCAAAGATTTGGAAACTCACTGAAATCAACGAACCGTCTCAATGTCGTTCCTTGAGGCTACCTGAAAATATGAGAGTAACCAAGATATCAAGATTAATCTATACAAATTCTGGTAATGCAATTCTGGCATTAGCATCGAATGCCATCCATTTGCTCTGGAAATGGCAAAGGAATGATCGCAATTCTGTTAGCAAGGCAACTGCCAGTGTTTCACCTCAACTCTGGCAGCCAACAAGTGGCATTCTAATGACAAATGATGTTACTGACACAAGTCCTGAAGAAGCTGTTCCTTGCTTTGCTTTATCCAAGAATGATTCTTATGTAATGTCGGCTTCTGGCGGAAAGATTTCCCTCTTCAATATGATGACATTTAAGACGATGACAACTTTCATGCCACCACCACCTGCAGCAACATTTCTTGCTTTCCATCCTCAAGATAACAATATTATAGCTATTGGCATGGATGATTCTACAATTCAGATATATAATGTCCGTGTGGATGAGGTTAAGAGCAAGCTTAAAGGTCACTCTAAAAGGATAACTGGCCTAGCATTCTCTCATGTATTAAATGTGCTAGTTTCATCAGGAGCAGATGCTCAGGTTTGTGTGTGGAATTCTGATGGATGGGAAAAACAGAAGAGCAGATTCTTGCAGCTTCCAGCTGGGAGAACACCATCTTCAGTGTCAGATACTCGCGTTCAGTTTCATCAGGATCAGGCACATTTCTTGGTTGTGCATGAGACTCAGCTGGCCATATTTGAAACAACAAAACTAGAATGTGTAAAGCAGTGGCTCCCACGTGAGTCTGCTGCCCCCATCTCCCATGCAACATTCTCATGCGATAGCCAGCTTATTTATGCCAGCTTTTTAGACGCAACCGTGTGCGTCTTCAGTGCTGCAAATCTCAGACTACGATGTCGTATTAATCCTCCTGTTTATCTTCCTGCCAATGTCAGCAGTTCTAATGTACAGCCCCTCGTGATTGCGGCACATCCACAAGAACCAAACCAGTTTGCTCTAGGACTATCAGATGGTGCGGTTCATGTGTTTGAGCCCCTTGAATCAGAAGGCAAATGGGGTGTGCCTCCACCTGTTGAAAATGGGTCTGCAAGCAGTTTGCCAGCTACTCAAGTTGGAAATTCAAGTTCTGATCAAGCTCAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009867 GO:0009888 GO:0009889 GO:0009890 GO:0009892 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010051 GO:0010072 GO:0010154 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022414 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032870 GO:0042221 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045892 GO:0045934 GO:0046983 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048519 GO:0048523 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:0090421 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04934 map04934

Pfam Domains

Protein Families

WD40

Protein Analysis

1139

Amino Acids

125.35

Weight (kDa)

6.6

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	5 - 34	6.8e-13	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	37 - 161	3.1e-64	TPR1-like, CTLH-containing domain
zf_topless	PF21359	164 - 204	1.2e-24	TOPLESS, zinc finger domain
Beta-prop_WDR3_1st	PF25173	394 - 534	4.7e-15	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	413 - 568	7.7e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	418 - 626	2.4e-22	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	418 - 674	1.5e-26	THOC3 beta-propeller domain
WDR55	PF24796	419 - 534	7.8e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	419 - 571	7e-10	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	431 - 537	4.9e-12	WDHD1 first WD40 domain
WD40	PF00400	457 - 491	2.7e-06	WD domain, G-beta repeat
WD40_Prp19	PF24814	463 - 537	1.5e-07	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	471 - 583	1.9e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	528 - 664	2.7e-06	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	554 - 672	8.1e-06	WDR5 beta-propeller domain
WD40_Prp19	PF24814	817 - 1042	5.4e-17	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	824 - 1059	4.2e-12	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	838 - 961	9.7e-13	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	841 - 964	8.2e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	841 - 957	2e-15	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	841 - 956	1.7e-09	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	843 - 1052	2.2e-17	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	844 - 1051	2.6e-20	WDR5 beta-propeller domain
WDR55	PF24796	844 - 956	1.4e-06	WDR55
Beta-prop_WDR3_2nd	PF25172	848 - 1048	3.6e-13	WDR3 second beta-propeller domain
Beta-prop_IFT140_1st	PF23383	860 - 960	2.2e-08	IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	861 - 956	2.4e-09	WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	865 - 961	2.4e-07	WDR90/POC16, second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1545
AarI	CACCTGC	2 cut(s)	652, 2665
AasI	GACNNNNNNGTC	1 cut(s)	3168
Acc36I	ACCTGC	3 cut(s)	652, 743, 2665
Acc65I	GGTACC	1 cut(s)	1118
AccB1I	GGYRCC	1 cut(s)	1118
AccB7I	CCANNNNNTGG	3 cut(s)	662, 680, 2489
AccI	GTMKAC	1 cut(s)	1496
AccII	CGCG	1 cut(s)	2949
AclWI	GGATC	9 cut(s)	609, 660, 673, 1149, 1162, 1235, 1248, 1627, 2973
AcoI	YGGCCR	1 cut(s)	3003
AcsI	RAATTY	7 cut(s)	59, 502, 1862, 1995, 2359, 2866, 3391
AcuI	CTGAAG	6 cut(s)	819, 2091, 2256, 2550, 2916, 3127
AcvI	CACGTG	1 cut(s)	3053
AfaI	GTAC	4 cut(s)	167, 1120, 1204, 3222
AflII	CTTAAG	1 cut(s)	440
AgeI	ACCGGT	2 cut(s)	267, 1827
AjnI	CCWGG	5 cut(s)	416, 1157, 1243, 1598, 1642
AjuI	GAANNNNNNNTTGG	2 cut(s)	859, 891
AleI	CACNNNNGTG	2 cut(s)	185, 1341
AloI	GAACNNNNNNTCC	2 cut(s)	11, 43
Alw21I	GWGCWC	3 cut(s)	631, 706, 1658
Alw26I	GTCTC	3 cut(s)	62, 2295, 2987
Alw44I	GTGCAC	2 cut(s)	702, 1654
AlwI	GGATC	9 cut(s)	609, 660, 673, 1149, 1162, 1235, 1248, 1627, 2973
AlwNI	CAGNNNCTG	2 cut(s)	797, 1105
Ama87I	CYCGRG	3 cut(s)	245, 630, 1623
AoxI	GGCC	7 cut(s)	569, 633, 710, 1596, 1915, 2797, 3003
ApaI	GGGCCC	2 cut(s)	637, 714
ApaLI	GTGCAC	2 cut(s)	702, 1654
ApoI	RAATTY	7 cut(s)	59, 502, 1862, 1995, 2359, 2866, 3391
AseI	ATTAAT	2 cut(s)	2348, 3177
AsiGI	ACCGGT	2 cut(s)	267, 1827
Asp700I	GAANNNNTTC	3 cut(s)	1554, 2538, 2599
Asp718I	GGTACC	1 cut(s)	1118
AspS9I	GGNCC	8 cut(s)	270, 633, 634, 710, 711, 824, 1767, 2064
AsuC2I	CCSGG	3 cut(s)	734, 987, 1922
AsuHPI	GGTGA	6 cut(s)	304, 633, 1355, 1730, 1856, 2457
AvaI	CYCGRG	3 cut(s)	245, 630, 1623
AvaII	GGWCC	4 cut(s)	270, 824, 1767, 2064
BaeGI	GKGCMC	4 cut(s)	637, 706, 714, 1658
BaeI	ACNNNNGTAYC	4 cut(s)	1110, 1143, 2934, 2967
BalI	TGGCCA	1 cut(s)	3005
BamHI	GGATCC	3 cut(s)	665, 1154, 1240
BanI	GGYRCC	1 cut(s)	1118
BanII	GRGCYC	4 cut(s)	637, 714, 1277, 3309
BauI	CACGAG	1 cut(s)	3230
BbrPI	CACGTG	1 cut(s)	3053
BbsI	GAAGAC	1 cut(s)	3133
Bbv12I	GWGCWC	3 cut(s)	631, 706, 1658
BceAI	ACGGC	1 cut(s)	1590
BcgI	CGANNNNNNTGC	2 cut(s)	2370, 2404
BciT130I	CCWGG	5 cut(s)	418, 1159, 1245, 1600, 1644
BciVI	GTATCC	1 cut(s)	1946
BclI	TGATCA	2 cut(s)	904, 3403
BcnI	CCSGG	3 cut(s)	734, 987, 1922
BcoDI	GTCTC	3 cut(s)	62, 2295, 2987
BfmI	CTRYAG	4 cut(s)	879, 1220, 2127, 2658
BfrI	CTTAAG	1 cut(s)	440
BfuAI	ACCTGC	3 cut(s)	652, 743, 2665
BfuI	GTATCC	1 cut(s)	1946
BmcAI	AGTACT	1 cut(s)	1204
Bme1390I	CCNGG	8 cut(s)	418, 734, 987, 1159, 1245, 1600, 1644, 1922
Bme18I	GGWCC	4 cut(s)	270, 824, 1767, 2064
BmeT110I	CYCGRG	3 cut(s)	245, 630, 1623
BmgT120I	GGNCC	8 cut(s)	270, 633, 634, 710, 711, 824, 1767, 2064
BmrFI	CCNGG	8 cut(s)	418, 734, 987, 1159, 1245, 1600, 1644, 1922
BoxI	GACNNNNGTC	1 cut(s)	2523
BpiI	GAAGAC	1 cut(s)	3133
BpmI	CTGGAG	1 cut(s)	702
Bpu10I	CCTNAGC	1 cut(s)	2850
BpuEI	CTTGAG	4 cut(s)	1291, 2326, 2670, 3366
BpuMI	CCSGG	3 cut(s)	734, 987, 1922
BsaAI	YACGTR	1 cut(s)	3053
BsaBI	GATNNNNATC	2 cut(s)	903, 1086
BsaJI	CCNNGG	7 cut(s)	417, 566, 732, 854, 930, 1158, 1662
BsaWI	WCCGGW	3 cut(s)	267, 1519, 1827
BsaXI	ACNNNNNCTCC	4 cut(s)	11, 41, 188, 218
Bse118I	RCCGGY	2 cut(s)	267, 1827
Bse1I	ACTGG	9 cut(s)	366, 974, 1173, 1486, 1584, 2154, 2457, 2800, 3262
Bse3DI	GCAATG	3 cut(s)	656, 2011, 2097
Bse8I	GATNNNNATC	2 cut(s)	903, 1086
BseBI	CCWGG	5 cut(s)	418, 1159, 1245, 1600, 1644
BseDI	CCNNGG	7 cut(s)	417, 566, 732, 854, 930, 1158, 1662
BseJI	GATNNNNATC	2 cut(s)	903, 1086
BseMI	GCAATG	3 cut(s)	656, 2011, 2097
BseMII	CTCAG	7 cut(s)	25, 362, 936, 2145, 2864, 3011, 3172
BseNI	ACTGG	9 cut(s)	366, 974, 1173, 1486, 1584, 2154, 2457, 2800, 3262
BseRI	GAGGAG	3 cut(s)	684, 819, 852
BseSI	GKGCMC	4 cut(s)	637, 706, 714, 1658
BseYI	CCCAGC	1 cut(s)	2915
Bsh1236I	CGCG	1 cut(s)	2949
BshFI	GGCC	7 cut(s)	571, 635, 712, 1598, 1917, 2799, 3005
BshNI	GGYRCC	1 cut(s)	1118
BshTI	ACCGGT	2 cut(s)	267, 1827
BsiHKAI	GWGCWC	3 cut(s)	631, 706, 1658
BsiHKCI	CYCGRG	3 cut(s)	245, 630, 1623
BsiSI	CCGG	6 cut(s)	268, 734, 986, 1520, 1828, 1922
BslFI	GGGAC	5 cut(s)	810, 948, 1145, 1717, 1883
BsmAI	GTCTC	3 cut(s)	62, 2295, 2987
BsmBI	CGTCTC	1 cut(s)	2295
BsmFI	GGGAC	5 cut(s)	810, 948, 1145, 1717, 1883
BsmI	GAATGC	5 cut(s)	1018, 1403, 2398, 2493, 2804
BsnI	GGCC	7 cut(s)	571, 635, 712, 1598, 1917, 2799, 3005
BsoBI	CYCGRG	3 cut(s)	245, 630, 1623
Bsp120I	GGGCCC	2 cut(s)	633, 710
Bsp1286I	GDGCHC	7 cut(s)	631, 637, 706, 714, 1277, 1658, 3309
Bsp1407I	TGTACA	1 cut(s)	3220
Bsp19I	CCATGG	2 cut(s)	930, 1662
BspANI	GGCC	7 cut(s)	571, 635, 712, 1598, 1917, 2799, 3005
BspCNI	CTCAG	7 cut(s)	24, 361, 937, 2146, 2863, 3010, 3171
BspFNI	CGCG	1 cut(s)	2949
BspHI	TCATGA	1 cut(s)	1510
BspMAI	CTGCAG	2 cut(s)	2131, 2662
BspMI	ACCTGC	3 cut(s)	652, 743, 2665
BspPI	GGATC	9 cut(s)	609, 660, 673, 1149, 1162, 1235, 1248, 1627, 2973
BspQI	GCTCTTC	1 cut(s)	2885
BspT107I	GGYRCC	1 cut(s)	1118
BspTI	CTTAAG	1 cut(s)	440
BsrDI	GCAATG	3 cut(s)	656, 2011, 2097
BsrFI	RCCGGY	2 cut(s)	267, 1827
BsrGI	TGTACA	1 cut(s)	3220
BsrI	ACTGG	9 cut(s)	366, 974, 1173, 1486, 1584, 2154, 2457, 2800, 3262
BssAI	RCCGGY	2 cut(s)	267, 1827
BssECI	CCNNGG	7 cut(s)	417, 566, 732, 854, 930, 1158, 1662
BssNAI	GTATAC	1 cut(s)	1497
BssSI	CACGAG	1 cut(s)	3230
BssT1I	CCWWGG	4 cut(s)	566, 854, 930, 1662
Bst1107I	GTATAC	1 cut(s)	1497
Bst2BI	CACGAG	1 cut(s)	3230
Bst2UI	CCWGG	5 cut(s)	418, 1159, 1245, 1600, 1644
Bst6I	CTCTTC	2 cut(s)	2612, 2885
BstAFI	CTTAAG	1 cut(s)	440
BstAPI	GCANNNNNTGC	3 cut(s)	1105, 1673, 2393
BstAUI	TGTACA	1 cut(s)	3220
BstBAI	YACGTR	1 cut(s)	3053
BstC8I	GCNNGC	9 cut(s)	1787, 1927, 2088, 2768, 3003, 3101, 3115, 3364, 3375
BstDSI	CCRYGG	2 cut(s)	930, 1662
BstENI	CCTNNNNNAGG	2 cut(s)	696, 750
BstFNI	CGCG	1 cut(s)	2949
BstMAI	GTCTC	3 cut(s)	62, 2295, 2987
BstNI	CCWGG	5 cut(s)	418, 1159, 1245, 1600, 1644
BstPAI	GACNNNNGTC	1 cut(s)	2523
BstSCI	CCNGG	8 cut(s)	416, 732, 985, 1157, 1243, 1598, 1642, 1920
BstSFI	CTRYAG	4 cut(s)	879, 1220, 2127, 2658
BstSLI	GKGCMC	4 cut(s)	637, 706, 714, 1658
BstUI	CGCG	1 cut(s)	2949
BstV2I	GAAGAC	1 cut(s)	3133
BstX2I	RGATCY	4 cut(s)	665, 1154, 1240, 1619
BstXI	CCANNNNNNTGG	2 cut(s)	280, 1078
BstYI	RGATCY	4 cut(s)	665, 1154, 1240, 1619
BstZ17I	GTATAC	1 cut(s)	1497
BsuI	GTATCC	1 cut(s)	1946
BsuRI	GGCC	7 cut(s)	571, 635, 712, 1598, 1917, 2799, 3005
BtgI	CCRYGG	2 cut(s)	930, 1662
BtgZI	GCGATG	1 cut(s)	1050
BtsI	GCAGTG	2 cut(s)	1764, 3047
BveI	ACCTGC	3 cut(s)	652, 743, 2665
Cac8I	GCNNGC	9 cut(s)	1787, 1927, 2088, 2768, 3003, 3101, 3115, 3364, 3375
CaiI	CAGNNNCTG	2 cut(s)	797, 1105
CciI	TCATGA	1 cut(s)	1510
Cfr10I	RCCGGY	2 cut(s)	267, 1827
Cfr13I	GGNCC	8 cut(s)	270, 633, 634, 710, 711, 824, 1767, 2064
CseI	GACGC	2 cut(s)	3127, 3134
Csp6I	GTAC	4 cut(s)	166, 1119, 1203, 3221
CspAI	ACCGGT	2 cut(s)	267, 1827
CviQI	GTAC	4 cut(s)	166, 1119, 1203, 3221
DraI	TTTAAA	2 cut(s)	303, 1053
DrdI	GACNNNNNNGTC	1 cut(s)	3168
DseDI	GACNNNNNNGTC	1 cut(s)	3168
EaeI	YGGCCR	1 cut(s)	3003
Eam1104I	CTCTTC	2 cut(s)	2612, 2885
EarI	CTCTTC	2 cut(s)	2612, 2885
EciI	GGCGGA	1 cut(s)	2607
Eco130I	CCWWGG	4 cut(s)	566, 854, 930, 1662
Eco147I	AGGCCT	2 cut(s)	1598, 1917
Eco24I	GRGCYC	4 cut(s)	637, 714, 1277, 3309
Eco32I	GATATC	1 cut(s)	2340
Eco47I	GGWCC	4 cut(s)	270, 824, 1767, 2064
Eco57I	CTGAAG	6 cut(s)	819, 2091, 2256, 2550, 2916, 3127
Eco72I	CACGTG	1 cut(s)	3053
Eco88I	CYCGRG	3 cut(s)	245, 630, 1623
EcoNI	CCTNNNNNAGG	2 cut(s)	696, 750
EcoO109I	RGGNCCY	4 cut(s)	634, 711, 1767, 2064
EcoRI	GAATTC	1 cut(s)	2866
EcoRII	CCWGG	5 cut(s)	416, 1157, 1243, 1598, 1642
EcoRV	GATATC	1 cut(s)	2340
EcoT14I	CCWWGG	4 cut(s)	566, 854, 930, 1662
EcoT22I	ATGCAT	2 cut(s)	2059, 2167
EcoT38I	GRGCYC	4 cut(s)	637, 714, 1277, 3309
ErhI	CCWWGG	4 cut(s)	566, 854, 930, 1662
Esp3I	CGTCTC	1 cut(s)	2295
FaqI	GGGAC	5 cut(s)	810, 948, 1145, 1717, 1883
FbaI	TGATCA	2 cut(s)	904, 3403
FblI	GTMKAC	1 cut(s)	1496
FriOI	GRGCYC	4 cut(s)	637, 714, 1277, 3309
GsaI	CCCAGC	1 cut(s)	2919
GsuI	CTGGAG	1 cut(s)	702
HaeIII	GGCC	7 cut(s)	571, 635, 712, 1598, 1917, 2799, 3005
HapII	CCGG	6 cut(s)	268, 734, 986, 1520, 1828, 1922
HgaI	GACGC	2 cut(s)	3127, 3134
HincII	GTYRAC	2 cut(s)	964, 1258
HindII	GTYRAC	2 cut(s)	964, 1258
HindIII	AAGCTT	2 cut(s)	532, 2768
HpaI	GTTAAC	2 cut(s)	964, 1258
HpaII	CCGG	6 cut(s)	268, 734, 986, 1520, 1828, 1922
HphI	GGTGA	6 cut(s)	304, 633, 1355, 1730, 1856, 2457
Hpy166II	GTNNAC	9 cut(s)	76, 180, 190, 283, 704, 964, 1258, 1497, 1656
Hpy8I	GTNNAC	9 cut(s)	76, 180, 190, 283, 704, 964, 1258, 1497, 1656
Hpy99I	CGWCG	1 cut(s)	1043
HpyAV	CCTTC	6 cut(s)	289, 794, 885, 1499, 1745, 3314
HpyCH4IV	ACGT	2 cut(s)	1038, 3052
HpySE526I	ACGT	2 cut(s)	1038, 3052
KpnI	GGTACC	1 cut(s)	1122
Ksp22I	TGATCA	2 cut(s)	904, 3403
KspAI	GTTAAC	2 cut(s)	964, 1258
LguI	GCTCTTC	1 cut(s)	2885
LmnI	GCTCC	8 cut(s)	19, 806, 1162, 1272, 1522, 1645, 2840, 3051
MaeII	ACGT	2 cut(s)	1038, 3052
MaeIII	GTNAC	9 cut(s)	639, 778, 976, 989, 995, 2077, 2329, 2512, 2777
MboII	GAAGA	9 cut(s)	97, 338, 2249, 2543, 2599, 2902, 2922, 3133, 3185
MfeI	CAATTG	1 cut(s)	145
MflI	RGATCY	4 cut(s)	665, 1154, 1240, 1619
MhlI	GDGCHC	7 cut(s)	631, 637, 706, 714, 1277, 1658, 3309
MlsI	TGGCCA	1 cut(s)	3005
MluNI	TGGCCA	1 cut(s)	3005
MlyI	GAGTC	5 cut(s)	853, 1096, 1730, 2989, 3065
MmeI	TCCRAC	4 cut(s)	737, 890, 1132, 3367
Mox20I	TGGCCA	1 cut(s)	3005
Mph1103I	ATGCAT	2 cut(s)	2059, 2167
MroXI	GAANNNNTTC	3 cut(s)	1554, 2538, 2599
MscI	TGGCCA	1 cut(s)	3005
MslI	CAYNNNNRTG	8 cut(s)	185, 290, 1076, 1341, 1383, 1661, 2498, 3088
Msp20I	TGGCCA	1 cut(s)	3005
MspA1I	CMGCKG	5 cut(s)	839, 2126, 2132, 2915, 3001
MspCI	CTTAAG	1 cut(s)	440
MspI	CCGG	6 cut(s)	268, 734, 986, 1520, 1828, 1922
MspR9I	CCNGG	8 cut(s)	418, 734, 987, 1159, 1245, 1600, 1644, 1922
MunI	CAATTG	1 cut(s)	145
Mva1269I	GAATGC	5 cut(s)	1018, 1403, 2398, 2493, 2804
MvaI	CCWGG	5 cut(s)	418, 1159, 1245, 1600, 1644
MvnI	CGCG	1 cut(s)	2949
NciI	CCSGG	3 cut(s)	734, 987, 1922
NcoI	CCATGG	2 cut(s)	930, 1662
NmuCI	GTSAC	6 cut(s)	639, 976, 989, 995, 2077, 2777
NsiI	ATGCAT	2 cut(s)	2059, 2167
OliI	CACNNNNGTG	2 cut(s)	185, 1341
PaeR7I	CTCGAG	2 cut(s)	245, 1623
PagI	TCATGA	1 cut(s)	1510
PaqCI	CACCTGC	2 cut(s)	652, 2665
PceI	AGGCCT	2 cut(s)	1598, 1917
PciSI	GCTCTTC	1 cut(s)	2885
PctI	GAATGC	5 cut(s)	1018, 1403, 2398, 2493, 2804
PdmI	GAANNNNTTC	3 cut(s)	1554, 2538, 2599
PflMI	CCANNNNNTGG	3 cut(s)	662, 680, 2489
PinAI	ACCGGT	2 cut(s)	267, 1827
PleI	GAGTC	5 cut(s)	853, 1096, 1729, 2989, 3064
PmaCI	CACGTG	1 cut(s)	3053
PmlI	CACGTG	1 cut(s)	3053
PpsI	GAGTC	5 cut(s)	853, 1096, 1729, 2989, 3064
Ppu21I	YACGTR	1 cut(s)	3053
PpuMI	RGGWCCY	2 cut(s)	1767, 2064
PshAI	GACNNNNGTC	1 cut(s)	2523
PshBI	ATTAAT	2 cut(s)	2348, 3177
PsiI	TTATAA	1 cut(s)	1545
Psp5II	RGGWCCY	2 cut(s)	1767, 2064
Psp6I	CCWGG	5 cut(s)	416, 1157, 1243, 1598, 1642
PspCI	CACGTG	1 cut(s)	3053
PspFI	CCCAGC	1 cut(s)	2915
PspGI	CCWGG	5 cut(s)	416, 1157, 1243, 1598, 1642
PspOMI	GGGCCC	2 cut(s)	633, 710
PspPI	GGNCC	8 cut(s)	270, 633, 634, 710, 711, 824, 1767, 2064
PspPPI	RGGWCCY	2 cut(s)	1767, 2064
PstI	CTGCAG	2 cut(s)	2131, 2662
PstNI	CAGNNNCTG	2 cut(s)	797, 1105
PsuI	RGATCY	4 cut(s)	665, 1154, 1240, 1619
PvuII	CAGCTG	4 cut(s)	839, 2132, 2915, 3001
RsaI	GTAC	4 cut(s)	167, 1120, 1204, 3222
RsaNI	GTAC	4 cut(s)	166, 1119, 1203, 3221
RseI	CAYNNNNRTG	8 cut(s)	185, 290, 1076, 1341, 1383, 1661, 2498, 3088
SapI	GCTCTTC	1 cut(s)	2885
Sau96I	GGNCC	8 cut(s)	270, 633, 634, 710, 711, 824, 1767, 2064
ScaI	AGTACT	1 cut(s)	1204
SchI	GAGTC	5 cut(s)	853, 1096, 1730, 2989, 3065
ScrFI	CCNGG	8 cut(s)	418, 734, 987, 1159, 1245, 1600, 1644, 1922
SduI	GDGCHC	7 cut(s)	631, 637, 706, 714, 1277, 1658, 3309
SfcI	CTRYAG	4 cut(s)	879, 1220, 2127, 2658
Sfr274I	CTCGAG	2 cut(s)	245, 1623
SinI	GGWCC	4 cut(s)	270, 824, 1767, 2064
SlaI	CTCGAG	2 cut(s)	245, 1623
SmiMI	CAYNNNNRTG	8 cut(s)	185, 290, 1076, 1341, 1383, 1661, 2498, 3088
SmlI	CTYRAG	7 cut(s)	245, 440, 1306, 1623, 2305, 2685, 3381
SmoI	CTYRAG	7 cut(s)	245, 440, 1306, 1623, 2305, 2685, 3381
SseBI	AGGCCT	2 cut(s)	1598, 1917
SspI	AATATT	4 cut(s)	119, 216, 1555, 2698
StuI	AGGCCT	2 cut(s)	1598, 1917
StyD4I	CCNGG	8 cut(s)	416, 732, 985, 1157, 1243, 1598, 1642, 1920
StyI	CCWWGG	4 cut(s)	566, 854, 930, 1662
TaiI	ACGT	2 cut(s)	1041, 3055
TaqI	TCGA	6 cut(s)	222, 246, 506, 830, 1624, 2390
TatI	WGTACW	2 cut(s)	1202, 3220
TauI	GCSGC	4 cut(s)	2120, 2123, 2126, 3242
TseFI	GTSAC	6 cut(s)	639, 976, 989, 995, 2077, 2777
Tsp45I	GTSAC	6 cut(s)	639, 976, 989, 995, 2077, 2777
TspGWI	ACGGA	3 cut(s)	461, 1353, 2735
Van91I	CCANNNNNTGG	3 cut(s)	662, 680, 2489
Vha464I	CTTAAG	1 cut(s)	440
VneI	GTGCAC	2 cut(s)	702, 1654
VpaK11BI	GGWCC	4 cut(s)	270, 824, 1767, 2064
VspI	ATTAAT	2 cut(s)	2348, 3177
XagI	CCTNNNNNAGG	2 cut(s)	696, 750
XapI	RAATTY	7 cut(s)	59, 502, 1862, 1995, 2359, 2866, 3391
XbaI	TCTAGA	1 cut(s)	46
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1165
XhoI	CTCGAG	2 cut(s)	245, 1623
XmiI	GTMKAC	1 cut(s)	1496
XmnI	GAANNNNTTC	3 cut(s)	1554, 2538, 2599
ZrmI	AGTACT	1 cut(s)	1204
Zsp2I	ATGCAT	2 cut(s)	2059, 2167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.