Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Reverse (-)

10950546 .. 10957632

Gene Structure 7087 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00170510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGGCGACGCCACCGGCGACAACGACAACACTGACTAAAGACCTCACCTTCTTGATCATGCAATTCCTTGACGAGGACAAGTGGAAGGATACGCTTCATAAGCTTCAGCAAGAATCAGGATACTTCTTCGACATGAAGTATTTCGAGGAATTATTGCTCGAGGGAAATTGGAATGAAGTCGAGAAGTATCTTTCCGGGTTCACTAAAGTGGATGACAACCGCTATTCGATGAAGATATTCTTCGAAATTCGTAAGCAGAAATACTTAGAAGCGCTGGATAGACATGATAGAGCTGCCGCAGTAGATATCCTTGTGAAGGATCTGAAAGTCTTTGCAGGTTTTCACGAAGACTTGTACAAGGAAATCACTCAGCTGCTCACTTTGAACAATTTTAGAGACAATGCTCAACTCTCTGCTTATGCAGATATAAGGACTGCAAGAGCAATTATGGCCCAAGAACTTAAGAAGCTTGTTGAGGCCAACCCTTCTTTCCGTGAAAAATTACAGTTCCCTAGCATTAAAAGTTCAAGGCTACGGACGCTCATTAACCAAAGCTTAAATTGGCAGCATTCCCTTTGTCCTAATCCTAAACAAAATCCGGAAATAAAAACCCTATTTGTGGATCACACTTGTAGACATTCCAACGATCCATTTGCTCATTTAGCAGCAAATGGTCAGGCTCAACAGATGGGTGCAGTACAGAGAGCTGATGGCTTCATTCCTATCAGTGTAAATGGGTCCTTCCAACCCCCAATAGCACCAGTACCAATTCCACCTCCTTTGTCAACATGGACGTCCATACCCCCCAGTGTCAATCAGTTGGCGGCTTCTGCAGGAGGTATTGGTTTTGGCAGTCCAGCAAACCCAGCTTCATATTTGTACAGTCCAGCAAATCCTGAAGATTTGTTCAGAACACGGTTCCCTGGGGTTTCAGACAGGGTTATGCTACCAGGGGTTAATCCAGGCCATGTTCTAAGTCCAGCATTCAATGCAATTGATGAGTTTCCGAAGGCTGTTGCCCGGATACTGAATCAGGGCTCAGTTCCCACTAGCATGGACTTCCATCCTCTTCAACAGACTATACTTCTAGTTGGAACTAGTATGGGGGATATAAGTGTCTGGGAAGTCAGTTCTAGGGAGAAGTTGGTATCAAGAAGCTTTCAGCCATGGGATATGGGGGCAAGTTCAATGATACTAAAGGCAACTTTAGTCAAGGATCCAAGTGTCTCAGTTAAGCGTGTACTATGGAGCCCTGATGGTTCTCTATTCGGAGTTGCATATTCTAAACACATGATGCAGTTATATGGTTATTTTGGTGGTGATGATGTTCGTCAGCATTTAGAGATCGATGCTCATGTTGGTAGTGTAAATGATCTGGCGTTCTGCAACCCAACTATGCAACTCTGTGTCATAACTTGCGGTGATGATAAGGCCATCAAGGTCTGGGATGCGGCTACTGGATCAAAATTGTATACTTTTGAAGGTCATGAGGCTCCTGTTCATTCTGTGTGCCCTCATAGCAAAGAACAGTTTGTCTTTTCGACATCAGTAGATGGACAAATAAAAGCATGGTTATATGAAGTGGTGGGGCCCAGGGTTAATTACGATGCTCCCAGCCTCTCATGCACAACGATGGTTTATAGTGCAGATGGTAAAAGGCTTTTTTCATGTGGGACGAGCAAAGATGGAGAATCTCATGTTGTTGAATGGAATGAAAATGAAGGCACAATAAAGAGGAATTACCAAGGATTTCAAAAACAATCTTTGGGTGTTGTTCAATTTGACACAACCAGGAATAAGTATCTAGCGGTTGGTGACGACTATGCAATCAAAGTTTGGGATATAGATAATATCAATCTTTTGGCTACTATTGATGCTGAAGGAGGTCTACCAGCAACCCCTCGTATCCGCTTCAACAAGGAAGGATCTTTGTTAGCTGTTTCTGCAAATGACAACAGAATCAAAGTTTTGGCTACAACAGATGGTCTCCAATTGATGCGCAATTATGAAAGTCTCTCGCTTATTGCCTCTAGAAATGCACCAGAAAAGAATGGTAGCACCAGAAGCATCGAGGATAATAAGAAACCAAAGTTAACTGAAGAGCTCAAACCTGCAAAGATCTGGAAGCCCACTGAGATTAGTGAAACTGCTCGGCTTCGGTCTTTAAGGCTCTCGACAACTACAGTTAAAACAGACAAGATCTCAAGGTTGGTTTACACTAATTCAGGTAATGCCATTCTGGCATTATCATCAAATGGAACCCATCTACTGTGGAGGTGGCCACAAGCTGACCGTAGTACGATTGGCAAGGCAACAACCAATGTTACCCCAAAATTCGTGGAACACGCTTCAGGCATCCTAATGATCAATGATCTTACTGGTGCTAAACCTGAAGATGCAATACCTTGCTTCGCTTTGTCCAAGAATGATTCATATGTCATGTCAGCCTCCGGAGGGAAGATATCGTTGTTCAACCTAATGACATTCAAGACGATGACAACTTTCATGTCGCCACCACCTGTGGTAACATGTCTTGCTTTCCACCCTGAAGACAATAATATAGTTGCTGTTGGATTTGATGATTCGACTATTCACATATACAATGTCCGTATCGATGAGGTTAGGATAAAGCTCAAGGGTCATTCTAAGAGAGTCACAGGCCTTGCGTTCTCTCAACTTCTGAAGACACTTGTTTCTTCTGGAGCAGATGCTCAGATAGTTGTGTGGAGCTCTGATAAGTGGGAAAGGCAGAAGAACAGTTTCTTGCAGGTTCCAGCTTGGACAACACCCACAGCACTGTTTGGAACTCATCTTCAGTATCAGAAGGATCAGAATCACTTCCTCGTTGTACATGAGACTCAACTTGCTGTATACGAAACCTTGAGGCTTGAATGCCAGAAGAAGTGGGTAGTAGGAGAATCTTCAGCACCAATCTCCTATGCAACATTCTCTTGTGATAGCCAGTTAGTCTACGCGAGCTTTCTTGATGGAACTGTGCGCGTGTTTGCAGCATCGAATCTTCAAATGCAATGCCAGATTAACCCCTATGCTTATCTTCCACCTAATGTCAGCTCTGCAACATACCCCCTAGTGATAGCTGCGAATCCACAAGAGCCGAACCAGTTTGCAGTGGGACTAACAGATGGTGCAGTTGTCATGTTTGAACCACTTGAGTCTGGAGACAAATGGGTTGTGCTGCCACCAGCAGACAATGGTTGCTTCCATGCTTCAACTTCTGCCAATGGTTCCAGTTTAGAACTCCAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

1089

Amino Acids

120.71

Weight (kDa)

6.43

Isoelectric Point (pI)

36.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LisH_TPL	PF17814	13 - 40	3.8e-09	LisH-like dimerisation domain
TPR1-like_2nd	PF21889	44 - 168	3.8e-58	TPR1-like, CTLH-containing domain
zf_topless	PF21359	171 - 211	2.4e-24	TOPLESS, zinc finger domain
WDR55	PF24796	348 - 524	3.3e-07	WDR55
Beta-prop_WDR3_1st	PF25173	390 - 558	3e-16	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	405 - 558	1.2e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	410 - 558	2.8e-15	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	410 - 666	8.6e-25	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	417 - 524	1.1e-10	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	448 - 630	2.3e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19	PF24814	448 - 658	2.6e-18	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	461 - 525	4.5e-07	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	462 - 526	2.1e-11	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	463 - 619	1.5e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	470 - 559	2.9e-07	WDR90/POC16, second beta-propeller
WD40	PF00400	488 - 524	5.2e-06	WD domain, G-beta repeat
WDR55	PF24796	498 - 656	2.2e-08	WDR55
Beta-prop_WDR5	PF25175	544 - 663	1.4e-06	WDR5 beta-propeller domain
WD40_Prp19	PF24814	725 - 996	2.3e-15	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	796 - 916	1.1e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	798 - 1006	2.9e-19	WDR5 beta-propeller domain
Beta-prop_WDR5	PF25175	798 - 913	5.2e-15	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	798 - 938	1.7e-12	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	800 - 1034	2.3e-07	MABP1/WDR62 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	803 - 1010	5.6e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	806 - 1006	3.3e-18	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	807 - 910	9.1e-10	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	808 - 917	3.9e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	812 - 910	9.1e-17	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	814 - 910	3.3e-11	WDR36/Utp21 first beta-propeller
Beta-prop_IFT140_1st	PF23383	814 - 922	2.5e-09	IFT140 first beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	819 - 913	4e-09	WDR90/POC16, second beta-propeller
Beta-prop_THOC3	PF25174	858 - 1013	6.9e-08	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	797
Acc16I	TGCGCA	1 cut(s)	2000
Acc36I	ACCTGC	3 cut(s)	326, 2119, 2758
AccI	GTMKAC	5 cut(s)	634, 1472, 1888, 2871, 2970
AccII	CGCG	2 cut(s)	2975, 3000
AccIII	TCCGGA	2 cut(s)	598, 2450
AciI	CCGC	7 cut(s)	220, 297, 824, 1419, 1451, 1808, 1909
AclWI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1933, 2835
AcoI	YGGCCR	1 cut(s)	2279
AcsI	RAATTY	2 cut(s)	246, 2333
AcyI	GRCGYC	2 cut(s)	8, 794
AfaI	GTAC	7 cut(s)	356, 699, 765, 881, 1242, 2299, 2850
AfeI	AGCGCT	1 cut(s)	273
AfiI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2454
AflII	CTTAAG	1 cut(s)	461
AflIII	ACRYGT	1 cut(s)	2528
AhlI	ACTAGT	1 cut(s)	1097
AjnI	CCWGG	5 cut(s)	922, 949, 961, 1592, 1790
AjuI	GAANNNNNNNTTGG	2 cut(s)	473, 505
AleI	CACNNNNGTG	1 cut(s)	206
Alw21I	GWGCWC	2 cut(s)	2106, 2732
Alw26I	GTCTC	6 cut(s)	390, 1231, 1991, 2018, 2849, 3174
AlwI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1933, 2835
Ama87I	CYCGRG	1 cut(s)	158
Aor13HI	TCCGGA	2 cut(s)	598, 2450
Aor51HI	AGCGCT	1 cut(s)	273
AoxI	GGCC	7 cut(s)	450, 477, 964, 1431, 1589, 2279, 2659
ApaI	GGGCCC	1 cut(s)	1593
ApeKI	GCWGC	7 cut(s)	293, 373, 565, 665, 3008, 3098, 3196
ApoI	RAATTY	2 cut(s)	246, 2333
Asp700I	GAANNNNTTC	4 cut(s)	140, 236, 2759, 2837
AspLEI	GCGC	3 cut(s)	274, 2001, 3000
AspS9I	GGNCC	4 cut(s)	451, 738, 1589, 1590
AsuC2I	CCSGG	2 cut(s)	196, 1021
AsuHPI	GGTGA	4 cut(s)	37, 1331, 1433, 1826
AsuII	TTCGAA	1 cut(s)	243
AvaI	CYCGRG	1 cut(s)	158
AvaII	GGWCC	1 cut(s)	738
BaeGI	GKGCMC	2 cut(s)	1514, 1593
BalI	TGGCCA	1 cut(s)	2281
BamHI	GGATCC	1 cut(s)	1216
BanII	GRGCYC	5 cut(s)	1040, 1253, 1593, 2106, 2732
BarI	GAAGNNNNNNTAC	4 cut(s)	1872, 1904, 2385, 2417
BbsI	GAAGAC	3 cut(s)	354, 2556, 2690
Bbv12I	GWGCWC	2 cut(s)	2106, 2732
BbvI	GCAGC	7 cut(s)	280, 360, 577, 677, 3020, 3085, 3183
BcgI	CGANNNNNNTGC	2 cut(s)	635, 669
BciT130I	CCWGG	5 cut(s)	924, 951, 963, 1594, 1792
BciVI	GTATCC	4 cut(s)	82, 113, 1017, 1916
BclI	TGATCA	2 cut(s)	54, 2364
BcnI	CCSGG	2 cut(s)	196, 1021
BcoDI	GTCTC	6 cut(s)	390, 1231, 1991, 2018, 2849, 3174
BcuI	ACTAGT	1 cut(s)	1097
BfaI	CTAG	8 cut(s)	513, 1050, 1088, 1098, 1134, 1805, 2031, 3089
BfmI	CTRYAG	2 cut(s)	831, 2181
BfoI	RGCGCY	1 cut(s)	275
BfrI	CTTAAG	1 cut(s)	461
BfuAI	ACCTGC	3 cut(s)	326, 2119, 2758
BfuI	GTATCC	4 cut(s)	82, 113, 1017, 1916
BglI	GCCNNNNNGGC	1 cut(s)	2240
BglII	AGATCT	2 cut(s)	2118, 2199
Bme1390I	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1594, 1792
Bme18I	GGWCC	1 cut(s)	738
BmeT110I	CYCGRG	1 cut(s)	158
BmgT120I	GGNCC	4 cut(s)	451, 738, 1589, 1590
BmrFI	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1594, 1792
BmrI	ACTGGG	1 cut(s)	801
BmuI	ACTGGG	1 cut(s)	801
BpiI	GAAGAC	3 cut(s)	354, 2556, 2690
BpmI	CTGGAG	3 cut(s)	2721, 3198, 3245
Bpu14I	TTCGAA	1 cut(s)	243
BpuEI	CTTGAG	4 cut(s)	2188, 2618, 2902, 3191
BpuMI	CCSGG	2 cut(s)	196, 1021
Bsa29I	ATCGAT	2 cut(s)	1347, 2613
BsaBI	GATNNNNATC	1 cut(s)	2832
BsaHI	GRCGYC	2 cut(s)	8, 794
BsaI	GGTCTC	1 cut(s)	1991
BsaJI	CCNNGG	7 cut(s)	922, 923, 950, 1166, 1592, 1593, 1744
BsaWI	WCCGGW	2 cut(s)	598, 2450
Bsc4I	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2454
Bse118I	RCCGGY	1 cut(s)	13
Bse1I	ACTGG	7 cut(s)	761, 807, 1462, 2383, 2962, 3121, 3249
Bse3DI	GCAATG	1 cut(s)	3035
Bse8I	GATNNNNATC	1 cut(s)	2832
BseAI	TCCGGA	2 cut(s)	598, 2450
BseBI	CCWGG	5 cut(s)	924, 951, 963, 1594, 1792
BseCI	ATCGAT	2 cut(s)	1347, 2613
BseDI	CCNNGG	7 cut(s)	922, 923, 950, 1166, 1592, 1593, 1744
BseGI	GGATG	4 cut(s)	217, 1063, 1453, 2355
BseJI	GATNNNNATC	1 cut(s)	2832
BseLI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2454
BseMI	GCAATG	1 cut(s)	3035
BseMII	CTCAG	5 cut(s)	383, 1053, 1242, 2124, 2726
BseNI	ACTGG	7 cut(s)	761, 807, 1462, 2383, 2962, 3121, 3249
BseSI	GKGCMC	2 cut(s)	1514, 1593
BseXI	GCAGC	7 cut(s)	280, 360, 577, 677, 3020, 3085, 3183
BseYI	CCCAGC	2 cut(s)	865, 1613
BsgI	GTGCAG	3 cut(s)	714, 1665, 3168
Bsh1236I	CGCG	2 cut(s)	2975, 3000
BshFI	GGCC	7 cut(s)	452, 479, 966, 1433, 1591, 2281, 2661
BshVI	ATCGAT	2 cut(s)	1347, 2613
BsiHKAI	GWGCWC	2 cut(s)	2106, 2732
BsiHKCI	CYCGRG	1 cut(s)	158
BsiSI	CCGG	5 cut(s)	14, 195, 599, 1021, 2451
BslFI	GGGAC	2 cut(s)	1687, 3147
BslI	CCNNNNNNNGG	4 cut(s)	73, 316, 619, 2454
BsmAI	GTCTC	6 cut(s)	390, 1231, 1991, 2018, 2849, 3174
BsmFI	GGGAC	2 cut(s)	1687, 3147
BsmI	GAATGC	3 cut(s)	568, 983, 2897
BsnI	GGCC	7 cut(s)	452, 479, 966, 1433, 1591, 2281, 2661
Bso31I	GGTCTC	1 cut(s)	1991
BsoBI	CYCGRG	1 cut(s)	158
Bsp119I	TTCGAA	1 cut(s)	243
Bsp120I	GGGCCC	1 cut(s)	1589
Bsp1286I	GDGCHC	6 cut(s)	1040, 1253, 1514, 1593, 2106, 2732
Bsp13I	TCCGGA	2 cut(s)	598, 2450
Bsp1407I	TGTACA	3 cut(s)	354, 879, 2848
Bsp19I	CCATGG	1 cut(s)	1166
BspACI	CCGC	7 cut(s)	220, 297, 824, 1419, 1451, 1808, 1909
BspANI	GGCC	7 cut(s)	452, 479, 966, 1433, 1591, 2281, 2661
BspCNI	CTCAG	5 cut(s)	382, 1052, 1241, 2125, 2725
BspDI	ATCGAT	2 cut(s)	1347, 2613
BspEI	TCCGGA	2 cut(s)	598, 2450
BspFNI	CGCG	2 cut(s)	2975, 3000
BspHI	TCATGA	1 cut(s)	1486
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	3 cut(s)	326, 2119, 2758
BspPI	GGATC	8 cut(s)	327, 630, 641, 1211, 1224, 1468, 1933, 2835
BspQI	GCTCTTC	1 cut(s)	2094
BspT104I	TTCGAA	1 cut(s)	243
BspTI	CTTAAG	1 cut(s)	461
BspTNI	GGTCTC	1 cut(s)	1991
BsrDI	GCAATG	1 cut(s)	3035
BsrFI	RCCGGY	1 cut(s)	13
BsrGI	TGTACA	3 cut(s)	354, 879, 2848
BsrI	ACTGG	7 cut(s)	761, 807, 1462, 2383, 2962, 3121, 3249
BssAI	RCCGGY	1 cut(s)	13
BssECI	CCNNGG	7 cut(s)	922, 923, 950, 1166, 1592, 1593, 1744
BssNAI	GTATAC	2 cut(s)	1473, 2872
BssNI	GRCGYC	2 cut(s)	8, 794
BssT1I	CCWWGG	2 cut(s)	1166, 1744
Bst1107I	GTATAC	2 cut(s)	1473, 2872
Bst2UI	CCWGG	5 cut(s)	924, 951, 963, 1594, 1792
Bst6I	CTCTTC	2 cut(s)	1074, 2094
BstACI	GRCGYC	2 cut(s)	8, 794
BstAFI	CTTAAG	1 cut(s)	461
BstAPI	GCANNNNNTGC	1 cut(s)	989
BstAUI	TGTACA	3 cut(s)	354, 879, 2848
BstBI	TTCGAA	1 cut(s)	243
BstC8I	GCNNGC	1 cut(s)	2977
BstDEI	CTNAG	8 cut(s)	265, 369, 974, 1039, 1228, 2133, 2646, 2712
BstDSI	CCRYGG	1 cut(s)	1166
BstENI	CCTNNNNNAGG	2 cut(s)	71, 314
BstF5I	GGATG	4 cut(s)	217, 1063, 1453, 2355
BstFNI	CGCG	2 cut(s)	2975, 3000
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	3 cut(s)	274, 2001, 3000
BstMAI	GTCTC	6 cut(s)	390, 1231, 1991, 2018, 2849, 3174
BstNI	CCWGG	5 cut(s)	924, 951, 963, 1594, 1792
BstNSI	RCATGY	1 cut(s)	2532
BstSCI	CCNGG	7 cut(s)	194, 922, 949, 961, 1019, 1592, 1790
BstSFI	CTRYAG	2 cut(s)	831, 2181
BstSLI	GKGCMC	2 cut(s)	1514, 1593
BstUI	CGCG	2 cut(s)	2975, 3000
BstV1I	GCAGC	7 cut(s)	280, 360, 577, 677, 3020, 3085, 3183
BstV2I	GAAGAC	3 cut(s)	354, 2556, 2690
BstX2I	RGATCY	5 cut(s)	319, 1216, 1925, 2118, 2199
BstXI	CCANNNNNNTGG	1 cut(s)	1054
BstYI	RGATCY	5 cut(s)	319, 1216, 1925, 2118, 2199
BstZ17I	GTATAC	2 cut(s)	1473, 2872
Bsu15I	ATCGAT	2 cut(s)	1347, 2613
BsuI	GTATCC	4 cut(s)	82, 113, 1017, 1916
BsuRI	GGCC	7 cut(s)	452, 479, 966, 1433, 1591, 2281, 2661
BsuTUI	ATCGAT	2 cut(s)	1347, 2613
BtgI	CCRYGG	1 cut(s)	1166
BtsCI	GGATG	4 cut(s)	217, 1063, 1453, 2355
BtsI	GCAGTG	1 cut(s)	3135
BtsIMutI	CAGTG	6 cut(s)	29, 733, 814, 2130, 2795, 3135
BveI	ACCTGC	3 cut(s)	326, 2119, 2758
Cac8I	GCNNGC	1 cut(s)	2977
CciI	TCATGA	1 cut(s)	1486
CfoI	GCGC	3 cut(s)	274, 2001, 3000
Cfr10I	RCCGGY	1 cut(s)	13
Cfr13I	GGNCC	4 cut(s)	451, 738, 1589, 1590
ClaI	ATCGAT	2 cut(s)	1347, 2613
CseI	GACGC	2 cut(s)	16, 547
Csp6I	GTAC	7 cut(s)	355, 698, 764, 880, 1241, 2298, 2849
CspCI	CAANNNNNGTGG	4 cut(s)	762, 797, 2319, 2354
CviQI	GTAC	7 cut(s)	355, 698, 764, 880, 1241, 2298, 2849
DdeI	CTNAG	8 cut(s)	265, 369, 974, 1039, 1228, 2133, 2646, 2712
EaeI	YGGCCR	1 cut(s)	2279
Eam1104I	CTCTTC	2 cut(s)	1074, 2094
EarI	CTCTTC	2 cut(s)	1074, 2094
Ecl136II	GAGCTC	2 cut(s)	2104, 2730
Eco130I	CCWWGG	2 cut(s)	1166, 1744
Eco147I	AGGCCT	1 cut(s)	2661
Eco24I	GRGCYC	5 cut(s)	1040, 1253, 1593, 2106, 2732
Eco31I	GGTCTC	1 cut(s)	1991
Eco32I	GATATC	2 cut(s)	307, 2463
Eco47I	GGWCC	1 cut(s)	738
Eco47III	AGCGCT	1 cut(s)	273
Eco53kI	GAGCTC	2 cut(s)	2104, 2730
Eco88I	CYCGRG	1 cut(s)	158
EcoICRI	GAGCTC	2 cut(s)	2104, 2730
EcoNI	CCTNNNNNAGG	2 cut(s)	71, 314
EcoO109I	RGGNCCY	2 cut(s)	738, 1589
EcoRII	CCWGG	5 cut(s)	922, 949, 961, 1592, 1790
EcoRV	GATATC	2 cut(s)	307, 2463
EcoT14I	CCWWGG	2 cut(s)	1166, 1744
EcoT38I	GRGCYC	5 cut(s)	1040, 1253, 1593, 2106, 2732
ErhI	CCWWGG	2 cut(s)	1166, 1744
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	2 cut(s)	1687, 3147
FauNDI	CATATG	1 cut(s)	2434
FbaI	TGATCA	2 cut(s)	54, 2364
FblI	GTMKAC	5 cut(s)	634, 1472, 1888, 2871, 2970
FokI	GGATG	4 cut(s)	224, 1050, 1460, 2342
FriOI	GRGCYC	5 cut(s)	1040, 1253, 1593, 2106, 2732
FspBI	CTAG	8 cut(s)	513, 1050, 1088, 1098, 1134, 1805, 2031, 3089
FspI	TGCGCA	1 cut(s)	2000
GlaI	GCGC	3 cut(s)	273, 2000, 2999
GsaI	CCCAGC	2 cut(s)	869, 1617
GsuI	CTGGAG	3 cut(s)	2721, 3198, 3245
HaeII	RGCGCY	1 cut(s)	275
HaeIII	GGCC	7 cut(s)	452, 479, 966, 1433, 1591, 2281, 2661
HapII	CCGG	5 cut(s)	14, 195, 599, 1021, 2451
HgaI	GACGC	2 cut(s)	16, 547
HhaI	GCGC	3 cut(s)	274, 2001, 3000
Hin1I	GRCGYC	2 cut(s)	8, 794
Hin6I	GCGC	3 cut(s)	272, 1999, 2998
HinP1I	GCGC	3 cut(s)	272, 1999, 2998
HincII	GTYRAC	2 cut(s)	786, 2094
HindII	GTYRAC	2 cut(s)	786, 2094
HindIII	AAGCTT	4 cut(s)	101, 467, 553, 1156
HpaI	GTTAAC	1 cut(s)	2094
HpaII	CCGG	5 cut(s)	14, 195, 599, 1021, 2451
HphI	GGTGA	4 cut(s)	37, 1331, 1433, 1826
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4IV	ACGT	1 cut(s)	794
HpyF3I	CTNAG	8 cut(s)	265, 369, 974, 1039, 1228, 2133, 2646, 2712
HpySE526I	ACGT	1 cut(s)	794
Hsp92I	GRCGYC	2 cut(s)	8, 794
HspAI	GCGC	3 cut(s)	272, 1999, 2998
Kpn2I	TCCGGA	2 cut(s)	598, 2450
Ksp22I	TGATCA	2 cut(s)	54, 2364
KspAI	GTTAAC	1 cut(s)	2094
LguI	GCTCTTC	1 cut(s)	2094
LmnI	GCTCC	5 cut(s)	1248, 1498, 1615, 2702, 2727
Lsp1109I	GCAGC	7 cut(s)	280, 360, 577, 677, 3020, 3085, 3183
MaeI	CTAG	8 cut(s)	513, 1050, 1088, 1098, 1134, 1805, 2031, 3089
MaeII	ACGT	1 cut(s)	794
MaeIII	GTNAC	4 cut(s)	1814, 2323, 2524, 2653
MfeI	CAATTG	2 cut(s)	993, 1991
MflI	RGATCY	5 cut(s)	319, 1216, 1925, 2118, 2199
MhlI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1593, 2106, 2732
MlsI	TGGCCA	1 cut(s)	2281
MluNI	TGGCCA	1 cut(s)	2281
MlyI	GAGTC	3 cut(s)	2661, 2851, 3182
MmeI	TCCRAC	4 cut(s)	666, 769, 1072, 2551
Mox20I	TGGCCA	1 cut(s)	2281
MroI	TCCGGA	2 cut(s)	598, 2450
MroXI	GAANNNNTTC	4 cut(s)	140, 236, 2759, 2837
MscI	TGGCCA	1 cut(s)	2281
MslI	CAYNNNNRTG	4 cut(s)	206, 1052, 1631, 2360
Msp20I	TGGCCA	1 cut(s)	2281
MspA1I	CMGCKG	1 cut(s)	373
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	5 cut(s)	14, 195, 599, 1021, 2451
MspR9I	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1594, 1792
MunI	CAATTG	2 cut(s)	993, 1991
Mva1269I	GAATGC	3 cut(s)	568, 983, 2897
MvaI	CCWGG	5 cut(s)	924, 951, 963, 1594, 1792
MvnI	CGCG	2 cut(s)	2975, 3000
NciI	CCSGG	2 cut(s)	196, 1021
NcoI	CCATGG	1 cut(s)	1166
NdeI	CATATG	1 cut(s)	2434
NmeAIII	GCCGAG	1 cut(s)	2131
NmuCI	GTSAC	2 cut(s)	1814, 2653
NsbI	TGCGCA	1 cut(s)	2000
NspI	RCATGY	1 cut(s)	2532
NspV	TTCGAA	1 cut(s)	243
OliI	CACNNNNGTG	1 cut(s)	206
PaeR7I	CTCGAG	1 cut(s)	158
PagI	TCATGA	1 cut(s)	1486
PasI	CCCWGGG	2 cut(s)	923, 1593
PceI	AGGCCT	1 cut(s)	2661
PciI	ACATGT	1 cut(s)	2528
PciSI	GCTCTTC	1 cut(s)	2094
PcsI	WCGNNNNNNNCGW	1 cut(s)	14
PctI	GAATGC	3 cut(s)	568, 983, 2897
PdmI	GAANNNNTTC	4 cut(s)	140, 236, 2759, 2837
PleI	GAGTC	3 cut(s)	2660, 2851, 3181
PpsI	GAGTC	3 cut(s)	2660, 2851, 3181
PpuMI	RGGWCCY	1 cut(s)	738
PscI	ACATGT	1 cut(s)	2528
Psp124BI	GAGCTC	2 cut(s)	2106, 2732
Psp5II	RGGWCCY	1 cut(s)	738
Psp6I	CCWGG	5 cut(s)	922, 949, 961, 1592, 1790
PspFI	CCCAGC	2 cut(s)	865, 1613
PspGI	CCWGG	5 cut(s)	922, 949, 961, 1592, 1790
PspOMI	GGGCCC	1 cut(s)	1589
PspPI	GGNCC	4 cut(s)	451, 738, 1589, 1590
PspPPI	RGGWCCY	1 cut(s)	738
PspXI	VCTCGAGB	1 cut(s)	158
PstI	CTGCAG	1 cut(s)	835
PsuI	RGATCY	5 cut(s)	319, 1216, 1925, 2118, 2199
PvuII	CAGCTG	1 cut(s)	373
RsaI	GTAC	7 cut(s)	356, 699, 765, 881, 1242, 2299, 2850
RsaNI	GTAC	7 cut(s)	355, 698, 764, 880, 1241, 2298, 2849
RseI	CAYNNNNRTG	4 cut(s)	206, 1052, 1631, 2360
SacI	GAGCTC	2 cut(s)	2106, 2732
SapI	GCTCTTC	1 cut(s)	2094
Sau96I	GGNCC	4 cut(s)	451, 738, 1589, 1590
SchI	GAGTC	3 cut(s)	2661, 2851, 3182
ScrFI	CCNGG	7 cut(s)	196, 924, 951, 963, 1021, 1594, 1792
SduI	GDGCHC	6 cut(s)	1040, 1253, 1514, 1593, 2106, 2732
SfcI	CTRYAG	2 cut(s)	831, 2181
Sfr274I	CTCGAG	1 cut(s)	158
SfuI	TTCGAA	1 cut(s)	243
SgrAI	CRCCGGYG	1 cut(s)	13
SinI	GGWCC	1 cut(s)	738
SlaI	CTCGAG	1 cut(s)	158
SmiMI	CAYNNNNRTG	4 cut(s)	206, 1052, 1631, 2360
SmlI	CTYRAG	6 cut(s)	158, 461, 2203, 2633, 2881, 3170
SmoI	CTYRAG	6 cut(s)	158, 461, 2203, 2633, 2881, 3170
SpeI	ACTAGT	1 cut(s)	1097
SseBI	AGGCCT	1 cut(s)	2661
SsiI	CCGC	7 cut(s)	220, 297, 824, 1419, 1451, 1808, 1909
SspMI	CTAG	8 cut(s)	513, 1050, 1088, 1098, 1134, 1805, 2031, 3089
SstI	GAGCTC	2 cut(s)	2106, 2732
StuI	AGGCCT	1 cut(s)	2661
StyD4I	CCNGG	7 cut(s)	194, 922, 949, 961, 1019, 1592, 1790
StyI	CCWWGG	2 cut(s)	1166, 1744
TaiI	ACGT	1 cut(s)	797
TaqII	GACCGA	1 cut(s)	2148
TatI	WGTACW	5 cut(s)	354, 697, 879, 1240, 2848
TauI	GCSGC	3 cut(s)	299, 827, 1454
TscAI	CASTG	6 cut(s)	36, 733, 814, 2137, 2802, 3135
TseFI	GTSAC	2 cut(s)	1814, 2653
TseI	GCWGC	7 cut(s)	293, 373, 565, 665, 3008, 3098, 3196
Tsp45I	GTSAC	2 cut(s)	1814, 2653
TspGWI	ACGGA	3 cut(s)	482, 550, 2597
TspRI	CASTG	6 cut(s)	36, 733, 814, 2137, 2802, 3135
Vha464I	CTTAAG	1 cut(s)	461
VpaK11BI	GGWCC	1 cut(s)	738
XagI	CCTNNNNNAGG	2 cut(s)	71, 314
XapI	RAATTY	2 cut(s)	246, 2333
XbaI	TCTAGA	1 cut(s)	2030
XceI	RCATGY	1 cut(s)	2532
XhoI	CTCGAG	1 cut(s)	158
XmiI	GTMKAC	5 cut(s)	634, 1472, 1888, 2871, 2970
XmnI	GAANNNNTTC	4 cut(s)	140, 236, 2759, 2837
XspI	CTAG	8 cut(s)	513, 1050, 1088, 1098, 1134, 1805, 2031, 3089
ZraI	GACGTC	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.