Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0000983

Physical Location & Seq

Genomic Coordinates Reverse (-)

16108062 .. 16112062

Gene Structure 4001 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1152000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1953 bp

ATGATGCTAAAGGCAACTCTGATCAAGGATCCATGCGTCTCAGTTAAGCGTATAATATGGAGTCCTGATGGTACTGTATTTGGAGTTGCATATTCTAAACACATTATCCAATTATACGATTATTTTGGCGGGAATGAGATTCACCAGCATTTGGAAATTGATGCTCACGCTGGCAGTGTTAATGATTTGGCGTTCGGCAACCCAGCTAAGCAACTTTGTGTCATAACTTGCGGTGATGATAAGGCCATTAAGGTTGGCTTTGAAGAAGTGGTGTGGAATGCAACTACTGGGTCAATGTTGTACACTTTCGAAGGTCATGATGCTCCCGTGCATTCTGTGTGTCCTCATAACAAGGAAAATGTCCATTTTATCTTTTCAACATCAGTAAATGGAAAAATAAAAGCATGGTTATACGACAATCTGGGATACCGGGTGGATTACAATGCTCCTGGTTACTCATGCACAACAATGGCCTATAGTGCAGATGGTAAAAGGCTATTTTCGTGCGGGACAAGCAAAGATGGAGAGTCTCATGTTGTTGAATGGAATGAAAATGATGGTGTAATAAAGAGGAATTACATAGGATTTCAGAAACAATCTTCAGGTGTTGTTCAGTTTGATACAAGCAAGAATAAGTATTTGGCTGTTGGAGATGACTATGCAATCAAAGTTTGGGATATGGATAAGACCAATATTTTGAGTACTATTGATGCTGAAGGTGGTCTACCAGCTAGTCCTTGCATTCGCTTCAACAAGGAGGGCTCTTTATTGGCTATTTCTGCAAACGACTGCAGAGTCAAAATTTTAGCTACGATGGATGGTCTTCGGCTGATGCGCACTATTGAAAGTCACTCGATTATTGCTTCAAGAAATGGACCTGAGACTGCAAGAAAGAGTGATAGCACCAGAAACCTCAAGGAGGTGAAACCAACCAGATTACCGGAAGAAGTGAACACAACAAGGATCTGGAATCTCACTCAGATCAGTGAAACTTCTCAGCTTCGGACATTGAGGCTCAGGGCCTTAATGAAAACAGACAAGATCTCAAGGTTGACATACACATATTCAGGCAATGCCATTCTAGCATTGGGATCCAGTGCCATCCATCAACTGTGGAGGTGGTCGCAGGATGACCATAGTTCGAGGGCAAAGACGATGACAACTTTCATGTCTCCACCCCTTGTGGCGACAAGTCTTGCTTTCCACCCTGAAGACAATAATGTTATTGCTGTTGGCATGGATGATTCCACTGTTCACATCTACAATGTCCGCATAAATGAGGTTAAGATCAAGCTTAAGGGTCACTCAAAAAGAGTCACGGTCTTGCCTTCTCCTATCTTCTTCATACACTTGTTTCTCTTGGAGCAGATGCTCAGGTTACTTATTGTGTGGAACTCTGAAAAGTGGGAAAGGGAGAATAGCTGTTTCTTGCGAATTCCTGCTGGGAGGATGCCTACAGCAATGGTTGAAACTCAGCTTCAGTATCACAAGGATCTGATGCACATCCTTGTAATACACGAGAGTCAACTTGCCATATATGAAACGTCGAAACTTGAATGTGAAAAGCAGTGGATTGTAGAAGAATCTGCAGCACCAATCTCGCACGCGACATTTTCTTGTGACAGCCAGTCAGTGTATGCGAGCTTTACGGATGGAACCGTGCGCATATTTACATCAAATCTTCAACTGCAATGCCAGATTAACCCCAATGCTTATTTTCCACCTGATGTCAGTTCTGCCTCACGCCCTCTGGTTGTAGCTGCACATCCCCAAGAGCCAAACCAGTTTGCCATAGGACTAACAGATGGAGCAGTTATTATCTTCGAACCGCTCAAATCAGAAGACAAATGGGGTGTGCCACCGCCTGTTGACAATGGCCTCCATACTACAAATGAAGGAAGCTTGGGTATTGGAAGCAAACGTTGGGTACAGGAAGCAAACGTCTCCTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0010014 GO:0010016 GO:0010072 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090421

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

651

Amino Acids

72.63

Weight (kDa)

6.52

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	11 - 136	5.6e-10	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	14 - 233	6.8e-16	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	14 - 279	8.6e-20	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	26 - 139	9.9e-06	WDHD1 first WD40 domain
WD40_Prp19	PF24814	53 - 269	3.2e-15	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	90 - 237	5.2e-12	WDR3 first beta-propeller domain
WDR55	PF24796	108 - 263	1.6e-07	WDR55
Beta-prop_WDR5	PF25175	153 - 285	3.3e-08	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	176 - 285	1.5e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	319 - 442	5.9e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	390 - 442	1.8e-06	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000983)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15750 AT1G15750 AT1G15750 AT1G15750 AT1G80490 AT1G80490 AT1G80490
fragaria_vesca	FvH4_2g28650 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170 FvH4_6g29170
malus_domestica	MD08G1152000.v1.1 MD09G1222700.v1.1 MD15G1129100.v1.1 MD17G1217100.v1.1
prunus_persica	Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.1G484400_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1 Prupe.3G093100_v2.0.a1
pyrus_communis	pycom08g13010 pycom15g11620 pycom17g22170 pycom808g00800
rosa_chinensis	RchiOBHm_Chr2g0134371 RchiOBHm_Chr6g0297431
rosa_laevigata	RLG00000011599 RLG00000019387
rosa_multiflora	Rmu_sc0004323.1_g000004 Rmu_ssc0000064.1_g000035
rosa_roxburghii	Rroxscaffold_2G00110290 Rroxscaffold_7G00170510
rosa_rugosa	Rorug02G0316200 Rorug02G0316300 Rorug02G0316400 Rorug02G0316500 Rorug02G0316600 Rorug06G0271900 Rorug06G0271900 Rorug06G0272000
rosa_samantha	Rh2AG368900 Rh2BG375100 Rh2CG352600 Rh2DG392200 Rh6AG383200 Rh6BG391600 Rh6CG396700 Rh6DG384000
rosa_wichuraiana	Rw2G030060 Rw6G033360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	794
Acc16I	TGCGCA	2 cut(s)	836, 1664
AccB7I	CCANNNNNTGG	1 cut(s)	151
AccBSI	CCGCTC	1 cut(s)	1831
AccI	GTMKAC	1 cut(s)	724
AccII	CGCG	1 cut(s)	1607
AciI	CCGC	6 cut(s)	129, 231, 507, 1270, 1829, 1862
AclI	AACGTT	1 cut(s)	1921
AclWI	GGATC	6 cut(s)	23, 36, 971, 1086, 1099, 1500
AcsI	RAATTY	2 cut(s)	801, 1434
AcuI	CTGAAG	4 cut(s)	585, 735, 1230, 1463
AfaI	GTAC	4 cut(s)	73, 302, 703, 1929
AfiI	CCNNNNNNNGG	2 cut(s)	151, 919
AflII	CTTAAG	1 cut(s)	1295
AgsI	TTSAA	9 cut(s)	263, 378, 542, 751, 845, 867, 1469, 1556, 1685
AhdI	GACNNNNNGTC	1 cut(s)	1627
AjnI	CCWGG	1 cut(s)	448
AjuI	GAANNNNNNNTTGG	4 cut(s)	623, 655, 1906, 1938
Alw26I	GTCTC	5 cut(s)	43, 534, 875, 1176, 1948
AlwI	GGATC	6 cut(s)	23, 36, 971, 1086, 1099, 1500
AoxI	GGCC	4 cut(s)	243, 471, 1020, 1876
ApeKI	GCWGC	2 cut(s)	1589, 1760
ApoI	RAATTY	2 cut(s)	801, 1434
ArsI	GACNNNNNNTTYG	2 cut(s)	679, 711
AspLEI	GCGC	2 cut(s)	837, 1665
AspS9I	GGNCC	2 cut(s)	875, 1020
AsuC2I	CCSGG	1 cut(s)	431
AsuHPI	GGTGA	3 cut(s)	134, 245, 934
AsuII	TTCGAA	2 cut(s)	309, 1824
AvaII	GGWCC	1 cut(s)	875
BamHI	GGATCC	2 cut(s)	28, 1091
BanII	GRGCYC	1 cut(s)	764
BarI	GAAGNNNNNNTAC	2 cut(s)	708, 740
BauI	CACGAG	1 cut(s)	1517
BbsI	GAAGAC	3 cut(s)	815, 1218, 1848
BbvI	GCAGC	2 cut(s)	1601, 1747
BcgI	CGANNNNNNTGC	2 cut(s)	1581, 1615
BciT130I	CCWGG	1 cut(s)	450
BciVI	GTATCC	1 cut(s)	419
BclI	TGATCA	1 cut(s)	21
BcnI	CCSGG	1 cut(s)	431
BcoDI	GTCTC	5 cut(s)	43, 534, 875, 1176, 1948
BfaI	CTAG	2 cut(s)	732, 1082
BfmI	CTRYAG	4 cut(s)	475, 790, 1455, 1587
BfrI	CTTAAG	1 cut(s)	1295
BfuI	GTATCC	1 cut(s)	419
BglII	AGATCT	1 cut(s)	1041
BisI	GCNGC	2 cut(s)	1590, 1761
BlpI	GCTNAGC	1 cut(s)	207
BlsI	GCNGC	2 cut(s)	1591, 1762
BmcAI	AGTACT	1 cut(s)	703
Bme1390I	CCNGG	2 cut(s)	431, 450
Bme18I	GGWCC	1 cut(s)	875
BmeRI	GACNNNNNGTC	1 cut(s)	1627
BmgT120I	GGNCC	2 cut(s)	875, 1020
BmiI	GGNNCC	3 cut(s)	30, 1093, 1657
BmrFI	CCNGG	2 cut(s)	431, 450
BmrI	ACTGGG	1 cut(s)	297
BmsI	GCATC	7 cut(s)	151, 310, 700, 822, 1359, 1440, 1488
BmuI	ACTGGG	1 cut(s)	297
BpiI	GAAGAC	3 cut(s)	815, 1218, 1848
Bpu10I	CCTNAGC	2 cut(s)	1016, 1373
Bpu1102I	GCTNAGC	1 cut(s)	207
Bpu14I	TTCGAA	2 cut(s)	309, 1824
BpuEI	CTTGAG	2 cut(s)	899, 1030
BpuMI	CCSGG	1 cut(s)	431
BsaBI	GATNNNNATC	1 cut(s)	1502
BsaWI	WCCGGW	1 cut(s)	940
Bsc4I	CCNNNNNNNGG	2 cut(s)	151, 919
Bse1I	ACTGG	4 cut(s)	292, 1095, 1627, 1783
Bse3DI	GCAATG	3 cut(s)	1078, 1467, 1697
Bse8I	GATNNNNATC	1 cut(s)	1502
BseBI	CCWGG	1 cut(s)	450
BseGI	GGATG	8 cut(s)	823, 1101, 1135, 1246, 1455, 1503, 1657, 1765
BseJI	GATNNNNATC	1 cut(s)	1502
BseLI	CCNNNNNNNGG	2 cut(s)	151, 919
BseMI	GCAATG	3 cut(s)	1078, 1467, 1697
BseMII	CTCAG	7 cut(s)	54, 870, 992, 1010, 1030, 1387, 1487
BseNI	ACTGG	4 cut(s)	292, 1095, 1627, 1783
BseXI	GCAGC	2 cut(s)	1601, 1747
BseYI	CCCAGC	2 cut(s)	202, 1442
BsgI	GTGCAG	2 cut(s)	501, 1746
Bsh1236I	CGCG	1 cut(s)	1607
BshFI	GGCC	4 cut(s)	245, 473, 1022, 1878
BsiSI	CCGG	2 cut(s)	430, 941
BslFI	GGGAC	1 cut(s)	523
BslI	CCNNNNNNNGG	2 cut(s)	151, 919
BsmAI	GTCTC	5 cut(s)	43, 534, 875, 1176, 1948
BsmBI	CGTCTC	2 cut(s)	43, 1948
BsmFI	GGGAC	1 cut(s)	523
BsmI	GAATGC	3 cut(s)	283, 331, 741
BsnI	GGCC	4 cut(s)	245, 473, 1022, 1878
Bsp119I	TTCGAA	2 cut(s)	309, 1824
Bsp1286I	GDGCHC	1 cut(s)	764
Bsp1407I	TGTACA	1 cut(s)	300
Bsp143I	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
Bsp1720I	GCTNAGC	1 cut(s)	207
BspACI	CCGC	6 cut(s)	129, 231, 507, 1270, 1829, 1862
BspANI	GGCC	4 cut(s)	245, 473, 1022, 1878
BspCNI	CTCAG	7 cut(s)	53, 871, 991, 1009, 1029, 1386, 1486
BspFNI	CGCG	1 cut(s)	1607
BspHI	TCATGA	1 cut(s)	316
BspLI	GGNNCC	3 cut(s)	30, 1093, 1657
BspMAI	CTGCAG	2 cut(s)	794, 1591
BspPI	GGATC	6 cut(s)	23, 36, 971, 1086, 1099, 1500
BspT104I	TTCGAA	2 cut(s)	309, 1824
BspTI	CTTAAG	1 cut(s)	1295
BsrBI	CCGCTC	1 cut(s)	1831
BsrDI	GCAATG	3 cut(s)	1078, 1467, 1697
BsrGI	TGTACA	1 cut(s)	300
BsrI	ACTGG	4 cut(s)	292, 1095, 1627, 1783
BssMI	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
BssSI	CACGAG	1 cut(s)	1517
Bst2BI	CACGAG	1 cut(s)	1517
Bst2UI	CCWGG	1 cut(s)	450
Bst4CI	ACNGT	5 cut(s)	76, 1113, 1252, 1321, 1660
BstAFI	CTTAAG	1 cut(s)	1295
BstAUI	TGTACA	1 cut(s)	300
BstBI	TTCGAA	2 cut(s)	309, 1824
BstC8I	GCNNGC	3 cut(s)	172, 1605, 1642
BstDEI	CTNAG	8 cut(s)	40, 207, 879, 978, 996, 1016, 1373, 1473
BstENI	CCTNNNNNAGG	1 cut(s)	917
BstF5I	GGATG	8 cut(s)	823, 1101, 1135, 1246, 1455, 1503, 1657, 1765
BstFNI	CGCG	1 cut(s)	1607
BstHHI	GCGC	2 cut(s)	837, 1665
BstKTI	GATC	8 cut(s)	24, 31, 966, 984, 1044, 1094, 1290, 1495
BstMAI	GTCTC	5 cut(s)	43, 534, 875, 1176, 1948
BstMBI	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
BstMWI	GCNNNNNNNGC	4 cut(s)	479, 513, 779, 1082
BstNI	CCWGG	1 cut(s)	450
BstSCI	CCNGG	2 cut(s)	429, 448
BstSFI	CTRYAG	4 cut(s)	475, 790, 1455, 1587
BstUI	CGCG	1 cut(s)	1607
BstV1I	GCAGC	2 cut(s)	1601, 1747
BstV2I	GAAGAC	3 cut(s)	815, 1218, 1848
BstX2I	RGATCY	5 cut(s)	28, 963, 1041, 1091, 1492
BstYI	RGATCY	5 cut(s)	28, 963, 1041, 1091, 1492
BsuI	GTATCC	1 cut(s)	419
BsuRI	GGCC	4 cut(s)	245, 473, 1022, 1878
BtsCI	GGATG	8 cut(s)	823, 1101, 1135, 1246, 1455, 1503, 1657, 1765
BtsI	GCAGTG	2 cut(s)	181, 1574
BtsIMutI	CAGTG	6 cut(s)	181, 991, 1102, 1248, 1574, 1638
Cac8I	GCNNGC	3 cut(s)	172, 1605, 1642
CciI	TCATGA	1 cut(s)	316
CfoI	GCGC	2 cut(s)	837, 1665
Cfr13I	GGNCC	2 cut(s)	875, 1020
CseI	GACGC	1 cut(s)	25
Csp6I	GTAC	4 cut(s)	72, 301, 702, 1928
CviAII	CATG	7 cut(s)	33, 317, 405, 459, 533, 1168, 1237
CviQI	GTAC	4 cut(s)	72, 301, 702, 1928
DdeI	CTNAG	8 cut(s)	40, 207, 879, 978, 996, 1016, 1373, 1473
DpnI	GATC	8 cut(s)	23, 30, 965, 983, 1043, 1093, 1289, 1494
DpnII	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
DrdI	GACNNNNNNGTC	1 cut(s)	794
DriI	GACNNNNNGTC	1 cut(s)	1627
DseDI	GACNNNNNNGTC	1 cut(s)	794
Eam1105I	GACNNNNNGTC	1 cut(s)	1627
Eco24I	GRGCYC	1 cut(s)	764
Eco47I	GGWCC	1 cut(s)	875
Eco57I	CTGAAG	4 cut(s)	585, 735, 1230, 1463
EcoNI	CCTNNNNNAGG	1 cut(s)	917
EcoO109I	RGGNCCY	1 cut(s)	1020
EcoRI	GAATTC	1 cut(s)	1434
EcoRII	CCWGG	1 cut(s)	448
EcoT38I	GRGCYC	1 cut(s)	764
Esp3I	CGTCTC	2 cut(s)	43, 1948
FaeI	CATG	7 cut(s)	36, 320, 408, 462, 536, 1171, 1240
FalI	AAGNNNNNCTT	4 cut(s)	242, 274, 1183, 1215
FaqI	GGGAC	1 cut(s)	523
FatI	CATG	7 cut(s)	32, 316, 404, 458, 532, 1167, 1236
FauI	CCCGC	2 cut(s)	122, 500
FbaI	TGATCA	1 cut(s)	21
FblI	GTMKAC	1 cut(s)	724
Fnu4HI	GCNGC	2 cut(s)	1590, 1761
FokI	GGATG	8 cut(s)	830, 1088, 1142, 1253, 1462, 1490, 1664, 1752
FriOI	GRGCYC	1 cut(s)	764
Fsp4HI	GCNGC	2 cut(s)	1590, 1761
FspAI	RTGCGCAY	2 cut(s)	836, 1664
FspBI	CTAG	2 cut(s)	732, 1082
FspI	TGCGCA	2 cut(s)	836, 1664
GlaI	GCGC	2 cut(s)	836, 1664
GluI	GCNGC	2 cut(s)	1590, 1761
GsaI	CCCAGC	2 cut(s)	206, 1446
HaeIII	GGCC	4 cut(s)	245, 473, 1022, 1878
HapII	CCGG	2 cut(s)	430, 941
HgaI	GACGC	1 cut(s)	25
HhaI	GCGC	2 cut(s)	837, 1665
Hin1II	CATG	7 cut(s)	36, 320, 408, 462, 536, 1171, 1240
Hin6I	GCGC	2 cut(s)	835, 1663
HinP1I	GCGC	2 cut(s)	835, 1663
HincII	GTYRAC	3 cut(s)	1053, 1526, 1870
HindII	GTYRAC	3 cut(s)	1053, 1526, 1870
HindIII	AAGCTT	2 cut(s)	1292, 1900
HinfI	GANTC	9 cut(s)	61, 139, 527, 795, 970, 1244, 1314, 1522, 1583
HpaII	CCGG	2 cut(s)	430, 941
HphI	GGTGA	3 cut(s)	134, 245, 934
Hpy166II	GTNNAC	7 cut(s)	303, 725, 952, 1053, 1255, 1526, 1870
Hpy188I	TCNGA	7 cut(s)	21, 591, 981, 1005, 1399, 1497, 1840
Hpy188III	TCNNGA	4 cut(s)	65, 317, 867, 967
Hpy8I	GTNNAC	7 cut(s)	303, 725, 952, 1053, 1255, 1526, 1870
Hpy99I	CGWCG	1 cut(s)	1549
HpyAV	CCTTC	4 cut(s)	305, 710, 1338, 1889
HpyCH4III	ACNGT	5 cut(s)	76, 1113, 1252, 1321, 1660
HpyCH4IV	ACGT	3 cut(s)	1544, 1921, 1941
HpyF10VI	GCNNNNNNNGC	4 cut(s)	479, 513, 779, 1082
HpyF3I	CTNAG	8 cut(s)	40, 207, 879, 978, 996, 1016, 1373, 1473
HpySE526I	ACGT	3 cut(s)	1544, 1921, 1941
Hsp92II	CATG	7 cut(s)	36, 320, 408, 462, 536, 1171, 1240
HspAI	GCGC	2 cut(s)	835, 1663
Ksp22I	TGATCA	1 cut(s)	21
Kzo9I	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
LmnI	GCTCC	4 cut(s)	328, 451, 1363, 1808
Lsp1109I	GCAGC	2 cut(s)	1601, 1747
LweI	GCATC	7 cut(s)	151, 310, 700, 822, 1359, 1440, 1488
MaeI	CTAG	2 cut(s)	732, 1082
MaeII	ACGT	3 cut(s)	1544, 1921, 1941
MaeIII	GTNAC	6 cut(s)	452, 848, 1301, 1315, 1377, 1619
MalI	GATC	8 cut(s)	23, 30, 965, 983, 1043, 1093, 1289, 1494
MbiI	CCGCTC	1 cut(s)	1831
MboI	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
MflI	RGATCY	5 cut(s)	28, 963, 1041, 1091, 1492
MhlI	GDGCHC	1 cut(s)	764
MluCI	AATT	5 cut(s)	110, 156, 574, 801, 1434
MlyI	GAGTC	5 cut(s)	70, 536, 804, 1323, 1531
MmeI	TCCRAC	1 cut(s)	628
MseI	TTAA	7 cut(s)	45, 180, 249, 1025, 1284, 1296, 1701
MslI	CAYNNNNRTG	1 cut(s)	467
MspCI	CTTAAG	1 cut(s)	1295
MspI	CCGG	2 cut(s)	430, 941
MspR9I	CCNGG	2 cut(s)	431, 450
Mva1269I	GAATGC	3 cut(s)	283, 331, 741
MvaI	CCWGG	1 cut(s)	450
MvnI	CGCG	1 cut(s)	1607
MwoI	GCNNNNNNNGC	4 cut(s)	479, 513, 779, 1082
NciI	CCSGG	1 cut(s)	431
NdeII	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
NlaIII	CATG	7 cut(s)	36, 320, 408, 462, 536, 1171, 1240
NlaIV	GGNNCC	3 cut(s)	30, 1093, 1657
NmuCI	GTSAC	4 cut(s)	848, 1301, 1315, 1619
NsbI	TGCGCA	2 cut(s)	836, 1664
NspV	TTCGAA	2 cut(s)	309, 1824
PagI	TCATGA	1 cut(s)	316
PctI	GAATGC	3 cut(s)	283, 331, 741
PfeI	GAWTC	4 cut(s)	139, 970, 1244, 1583
PflMI	CCANNNNNTGG	1 cut(s)	151
PkrI	GCNGC	2 cut(s)	1591, 1762
PleI	GAGTC	5 cut(s)	69, 535, 803, 1322, 1530
PpsI	GAGTC	5 cut(s)	69, 535, 803, 1322, 1530
Psp1406I	AACGTT	1 cut(s)	1921
Psp6I	CCWGG	1 cut(s)	448
PspFI	CCCAGC	2 cut(s)	202, 1442
PspGI	CCWGG	1 cut(s)	448
PspN4I	GGNNCC	3 cut(s)	30, 1093, 1657
PspPI	GGNCC	2 cut(s)	875, 1020
PstI	CTGCAG	2 cut(s)	794, 1591
PsuI	RGATCY	5 cut(s)	28, 963, 1041, 1091, 1492
RsaI	GTAC	4 cut(s)	73, 302, 703, 1929
RsaNI	GTAC	4 cut(s)	72, 301, 702, 1928
RseI	CAYNNNNRTG	1 cut(s)	467
SaqAI	TTAA	7 cut(s)	45, 180, 249, 1025, 1284, 1296, 1701
SatI	GCNGC	2 cut(s)	1590, 1761
Sau3AI	GATC	8 cut(s)	21, 28, 963, 981, 1041, 1091, 1287, 1492
Sau96I	GGNCC	2 cut(s)	875, 1020
ScaI	AGTACT	1 cut(s)	703
SchI	GAGTC	5 cut(s)	70, 536, 804, 1323, 1531
ScrFI	CCNGG	2 cut(s)	431, 450
SduI	GDGCHC	1 cut(s)	764
SfaNI	GCATC	7 cut(s)	151, 310, 700, 822, 1359, 1440, 1488
SfcI	CTRYAG	4 cut(s)	475, 790, 1455, 1587
SfuI	TTCGAA	2 cut(s)	309, 1824
SinI	GGWCC	1 cut(s)	875
SmiMI	CAYNNNNRTG	1 cut(s)	467
SmlI	CTYRAG	3 cut(s)	914, 1045, 1295
SmoI	CTYRAG	3 cut(s)	914, 1045, 1295
Sse9I	AATT	5 cut(s)	110, 156, 574, 801, 1434
SsiI	CCGC	6 cut(s)	129, 231, 507, 1270, 1829, 1862
SspI	AATATT	1 cut(s)	694
SspMI	CTAG	2 cut(s)	732, 1082
StyD4I	CCNGG	2 cut(s)	429, 448
TaaI	ACNGT	5 cut(s)	76, 1113, 1252, 1321, 1660
TaiI	ACGT	3 cut(s)	1547, 1924, 1944
TaqI	TCGA	5 cut(s)	309, 854, 1142, 1547, 1824
TasI	AATT	5 cut(s)	110, 156, 574, 801, 1434
TatI	WGTACW	2 cut(s)	300, 701
TfiI	GAWTC	4 cut(s)	139, 970, 1244, 1583
Tru1I	TTAA	7 cut(s)	45, 180, 249, 1025, 1284, 1296, 1701
Tru9I	TTAA	7 cut(s)	45, 180, 249, 1025, 1284, 1296, 1701
TscAI	CASTG	6 cut(s)	181, 991, 1102, 1255, 1574, 1638
TseFI	GTSAC	4 cut(s)	848, 1301, 1315, 1619
TseI	GCWGC	2 cut(s)	1589, 1760
Tsp45I	GTSAC	4 cut(s)	848, 1301, 1315, 1619
TspDTI	ATGAA	6 cut(s)	564, 1043, 1156, 1333, 1554, 1908
TspGWI	ACGGA	1 cut(s)	1664
TspRI	CASTG	6 cut(s)	181, 991, 1102, 1255, 1574, 1638
Van91I	CCANNNNNTGG	1 cut(s)	151
Vha464I	CTTAAG	1 cut(s)	1295
VpaK11BI	GGWCC	1 cut(s)	875
XagI	CCTNNNNNAGG	1 cut(s)	917
XapI	RAATTY	2 cut(s)	801, 1434
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1084
XmiI	GTMKAC	1 cut(s)	724
XspI	CTAG	2 cut(s)	732, 1082
ZrmI	AGTACT	1 cut(s)	703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.