FvH4_3g01611

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004190
Physical Location & Seq
Reverse (-)
847082 .. 849454
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01611.t1

Sequence Viewer

Length: 1242 bp
ATGATTGTTTTGATATCTTCCCAGGTGCTGAGGACTTTCTTGTTTTTGTTCTTGTTGGCATGGATCACAAAAGGCGATGATCTTAATCCCCTTCGTGTATCAGAACGTGATGGTCGACCCATGGTTTTCAATGTGATGCGTTATGGAGCTGTTGCAGACGGAAGAACTGATAACAGCCAGGCGTTTTTGAAAGCATGGAGTGAAGCCTGTAACTGGGATGGAAGAGCAAGGGTTGTGATTCCTTCCAAAACATTCAAGTTATTCCCGGTTCTTTTCTCTGGTCCATGCAAGGGTCGCATTGCCTTTATAATCAAGGGAACTTTGCTGGCTTCTAATAGTCCATCAGCAGTGAGTTCTGAAAACTGGATAAACTTTGAGAACGTCGACCAACTGACGGTGGCCGGAGGCGGTACGCTAGACGCTCAAGGACCCTCTGTTTGGTCTCTTAACGATTGCAATACTAATCCAGGATGCAAAGCTCTCCCAATTTCTATGACTTTCAACTTCGTCTCAAATGCAAGCGTACATCACTTGAGATCATTCAACAGTCAAAATGGTCATTTCAAGGTTCATGGATGCCAGAAGATAGACTTCCGGAATATAAGAATATCGGCTCCGGAGGACAGTCCAAACACCGATGGCATAAAAATCGGAAACTCATACCGCGTTAGAATCAGACACACGATCATTCAGACCGGTGATGATTGTATAGCAATCGTGTCTGGAAGTCGGAAAATCCACATTTCTAATGTATTTTGCGGTCCTGGACATGGAATCAGCATCGGAAGTCTTGGAGGGCATGCTTATACAGAATATGACGTGGAGGGAATTTTTGTGAAAAATAGTGTTTTCAAAAAGACTGCTAATGGTTTGAGAATCAAAACATATGCTAGGGATTCAAAACCCTTGAAGGCTTCAAATATTGTCTTTGAAGATATTAACATGATTGATGTCATGTATCCCATTATCATAGATCAACAATATTGTCCACACAATCAATGCGATCTACAGGATTCTTCATCAAACGTGCAAGTCAGCGATGTTACATTCAGAAACATATGGGGAACCTCGTCTTCCAAAGAAGCAGTGACTCTGAAATGTAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATCAAGTTACCTCATAATGGTCCAAAAGGACCTGCCACTTCCGTCTGCAGTAATGTCATCGGCAACTCTTATGGCTCACACATCCCTCCTTCTTGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

414

Amino Acids

45.25

Weight (kDa)

9.04

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 73 - 399 2.1e-93 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 308
Acc36I ACCTGC 1 cut(s) 1180
AccI GTMKAC 2 cut(s) 115, 384
AccII CGCG 1 cut(s) 666
AccIII TCCGGA 2 cut(s) 594, 616
AciI CCGC 3 cut(s) 408, 664, 759
AclWI GGATC 1 cut(s) 71
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 1 cut(s) 828
AfaI GTAC 2 cut(s) 412, 525
AfiI CCNNNNNNNGG 6 cut(s) 213, 290, 394, 438, 770, 1157
AgeI ACCGGT 1 cut(s) 695
AjiI CACGTC 1 cut(s) 820
AjnI CCWGG 4 cut(s) 21, 177, 466, 763
AluBI AGCT 2 cut(s) 149, 479
AluI AGCT 2 cut(s) 149, 479
Alw26I GTCTC 2 cut(s) 447, 514
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 28
Aor13HI TCCGGA 2 cut(s) 594, 616
AoxI GGCC 1 cut(s) 399
ApoI RAATTY 1 cut(s) 828
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AsiGI ACCGGT 1 cut(s) 695
AspS9I GGNCC 5 cut(s) 281, 428, 761, 1160, 1169
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 710
AvaII GGWCC 5 cut(s) 281, 428, 761, 1160, 1169
BbsI GAAGAC 1 cut(s) 1065
BbvCI CCTCAGC 1 cut(s) 29
BccI CCATC 5 cut(s) 104, 212, 349, 632, 1126
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BciT130I CCWGG 4 cut(s) 23, 179, 468, 765
BciVI GTATCC 1 cut(s) 969
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 447, 514
BfaI CTAG 2 cut(s) 416, 891
BfmI CTRYAG 2 cut(s) 1007, 1186
BfuAI ACCTGC 1 cut(s) 1180
BfuI GTATCC 1 cut(s) 969
Bme1390I CCNGG 5 cut(s) 23, 179, 266, 468, 765
Bme18I GGWCC 5 cut(s) 281, 428, 761, 1160, 1169
BmgBI CACGTC 1 cut(s) 820
BmgT120I GGNCC 5 cut(s) 281, 428, 761, 1160, 1169
BmiI GGNNCC 3 cut(s) 430, 615, 1066
BmrFI CCNGG 5 cut(s) 23, 179, 266, 468, 765
BmrI ACTGGG 1 cut(s) 223
BmsI GCATC 4 cut(s) 126, 461, 566, 789
BmuI ACTGGG 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 1065
Bpu10I CCTNAGC 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 408, 553
BpuMI CCSGG 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 21, 120
BsaWI WCCGGW 3 cut(s) 594, 616, 695
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 290, 394, 438, 770, 1157
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 2 cut(s) 218, 368
Bse3DI GCAATG 1 cut(s) 297
Bse8I GATNNNNATC 1 cut(s) 84
BseAI TCCGGA 2 cut(s) 594, 616
BseBI CCWGG 4 cut(s) 23, 179, 468, 765
BseDI CCNNGG 2 cut(s) 21, 120
BseGI GGATG 4 cut(s) 223, 476, 581, 1221
BseJI GATNNNNATC 1 cut(s) 84
BseLI CCNNNNNNNGG 6 cut(s) 213, 290, 394, 438, 770, 1157
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 218, 368
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 695
BsiSI CCGG 5 cut(s) 266, 402, 595, 617, 696
BslI CCNNNNNNNGG 6 cut(s) 213, 290, 394, 438, 770, 1157
BsmAI GTCTC 2 cut(s) 447, 514
BsmBI CGTCTC 1 cut(s) 514
BsnI GGCC 1 cut(s) 401
Bso31I GGTCTC 1 cut(s) 447
Bsp13I TCCGGA 2 cut(s) 594, 616
Bsp143I GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 3 cut(s) 408, 664, 759
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 1 cut(s) 21
BspEI TCCGGA 2 cut(s) 594, 616
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 3 cut(s) 430, 615, 1066
BspMAI CTGCAG 1 cut(s) 1190
BspMI ACCTGC 1 cut(s) 1180
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 2 cut(s) 218, 368
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 2 cut(s) 21, 120
BssMI GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 4 cut(s) 23, 179, 468, 765
Bst4CI ACNGT 3 cut(s) 397, 548, 626
Bst6I CTCTTC 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 1116
BstC8I GCNNGC 4 cut(s) 327, 520, 801, 1112
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 4 cut(s) 223, 476, 581, 1221
BstFNI CGCG 1 cut(s) 666
BstKTI GATC 6 cut(s) 66, 82, 539, 687, 976, 1006
BstMAI GTCTC 2 cut(s) 447, 514
BstMBI GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
BstMWI GCNNNNNNNGC 2 cut(s) 294, 1116
BstNI CCWGG 4 cut(s) 23, 179, 468, 765
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 5 cut(s) 21, 177, 264, 466, 763
BstSFI CTRYAG 2 cut(s) 1007, 1186
BstUI CGCG 1 cut(s) 666
BstV2I GAAGAC 1 cut(s) 1065
BsuI GTATCC 1 cut(s) 969
BsuRI GGCC 1 cut(s) 401
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 2 cut(s) 90, 1053
BtrI CACGTC 1 cut(s) 820
BtsCI GGATG 4 cut(s) 223, 476, 581, 1221
BtsI GCAGTG 2 cut(s) 354, 1092
BtsIMutI CAGTG 2 cut(s) 354, 1092
BveI ACCTGC 1 cut(s) 1180
Cac8I GCNNGC 4 cut(s) 327, 520, 801, 1112
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 5 cut(s) 281, 428, 761, 1160, 1169
CseI GACGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 411, 524
CspAI ACCGGT 1 cut(s) 695
CviQI GTAC 2 cut(s) 411, 524
DdeI CTNAG 1 cut(s) 29
DpnI GATC 6 cut(s) 65, 81, 538, 686, 975, 1005
DpnII GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 447
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 5 cut(s) 281, 428, 761, 1160, 1169
EcoO109I RGGNCCY 2 cut(s) 428, 1169
EcoRII CCWGG 4 cut(s) 21, 177, 466, 763
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 514
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FauNDI CATATG 2 cut(s) 886, 1058
FblI GTMKAC 2 cut(s) 115, 384
FokI GGATG 4 cut(s) 230, 483, 588, 1208
FspBI CTAG 2 cut(s) 416, 891
HaeIII GGCC 1 cut(s) 401
HapII CCGG 5 cut(s) 266, 402, 595, 617, 696
HgaI GACGC 1 cut(s) 428
HincII GTYRAC 2 cut(s) 116, 385
HindII GTYRAC 2 cut(s) 116, 385
HinfI GANTC 7 cut(s) 238, 672, 774, 876, 896, 1013, 1090
HpaII CCGG 5 cut(s) 266, 402, 595, 617, 696
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 3 cut(s) 116, 385, 989
Hpy188I TCNGA 9 cut(s) 103, 358, 653, 677, 693, 732, 785, 1052, 1095
Hpy188III TCNNGA 3 cut(s) 595, 617, 723
Hpy8I GTNNAC 3 cut(s) 116, 385, 989
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 4 cut(s) 101, 252, 904, 1239
HpyCH4III ACNGT 3 cut(s) 397, 548, 626
HpyCH4IV ACGT 4 cut(s) 106, 381, 819, 1026
HpyCH4V TGCA 9 cut(s) 155, 288, 456, 474, 518, 1030, 1119, 1188, 1236
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 1116
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 4 cut(s) 106, 381, 819, 1026
Kpn2I TCCGGA 2 cut(s) 594, 616
Kzo9I GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
LguI GCTCTTC 1 cut(s) 217
LmnI GCTCC 2 cut(s) 146, 619
LweI GCATC 4 cut(s) 126, 461, 566, 789
MaeI CTAG 2 cut(s) 416, 891
MaeII ACGT 4 cut(s) 106, 381, 819, 1026
MaeIII GTNAC 4 cut(s) 209, 1042, 1087, 1146
MalI GATC 6 cut(s) 65, 81, 538, 686, 975, 1005
MboI GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
MboII GAAGA 7 cut(s) 9, 174, 234, 595, 944, 1008, 1065
MluCI AATT 2 cut(s) 486, 828
MlyI GAGTC 1 cut(s) 1084
MmeI TCCRAC 1 cut(s) 710
MnlI CCTC 9 cut(s) 24, 398, 442, 613, 788, 817, 1078, 1161, 1236
MroI TCCGGA 2 cut(s) 594, 616
MseI TTAA 3 cut(s) 84, 447, 939
MspI CCGG 5 cut(s) 266, 402, 595, 617, 696
MspR9I CCNGG 5 cut(s) 23, 179, 266, 468, 765
MvaI CCWGG 4 cut(s) 23, 179, 468, 765
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 2 cut(s) 294, 1116
NciI CCSGG 1 cut(s) 266
NcoI CCATGG 1 cut(s) 120
NdeI CATATG 2 cut(s) 886, 1058
NdeII GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
NlaIV GGNNCC 3 cut(s) 430, 615, 1066
NmuCI GTSAC 1 cut(s) 1087
NspI RCATGY 1 cut(s) 803
PaeI GCATGC 1 cut(s) 803
PciSI GCTCTTC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 112
PfeI GAWTC 6 cut(s) 238, 672, 774, 876, 896, 1013
PfoI TCCNGGA 2 cut(s) 466, 763
PinAI ACCGGT 1 cut(s) 695
PleI GAGTC 1 cut(s) 1084
PpsI GAGTC 1 cut(s) 1084
PpuMI RGGWCCY 2 cut(s) 428, 1169
PsiI TTATAA 1 cut(s) 308
Psp5II RGGWCCY 2 cut(s) 428, 1169
Psp6I CCWGG 4 cut(s) 21, 177, 466, 763
PspGI CCWGG 4 cut(s) 21, 177, 466, 763
PspN4I GGNNCC 3 cut(s) 430, 615, 1066
PspPI GGNCC 5 cut(s) 281, 428, 761, 1160, 1169
PspPPI RGGWCCY 2 cut(s) 428, 1169
PstI CTGCAG 1 cut(s) 1190
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 2 cut(s) 412, 525
RsaNI GTAC 2 cut(s) 411, 524
SalI GTCGAC 2 cut(s) 114, 383
SapI GCTCTTC 1 cut(s) 217
SaqAI TTAA 3 cut(s) 84, 447, 939
Sau3AI GATC 6 cut(s) 63, 79, 536, 684, 973, 1003
Sau96I GGNCC 5 cut(s) 281, 428, 761, 1160, 1169
SchI GAGTC 1 cut(s) 1084
ScrFI CCNGG 5 cut(s) 23, 179, 266, 468, 765
SfaNI GCATC 4 cut(s) 126, 461, 566, 789
SfcI CTRYAG 2 cut(s) 1007, 1186
SinI GGWCC 5 cut(s) 281, 428, 761, 1160, 1169
SmlI CTYRAG 2 cut(s) 423, 532
SmoI CTYRAG 2 cut(s) 423, 532
SphI GCATGC 1 cut(s) 803
Sse9I AATT 2 cut(s) 486, 828
SsiI CCGC 3 cut(s) 408, 664, 759
SspI AATATT 2 cut(s) 922, 983
SspMI CTAG 2 cut(s) 416, 891
StyD4I CCNGG 5 cut(s) 21, 177, 264, 466, 763
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 397, 548, 626
TaiI ACGT 4 cut(s) 109, 384, 822, 1029
TaqI TCGA 2 cut(s) 115, 384
TasI AATT 2 cut(s) 486, 828
TfiI GAWTC 6 cut(s) 238, 672, 774, 876, 896, 1013
Tru1I TTAA 3 cut(s) 84, 447, 939
Tru9I TTAA 3 cut(s) 84, 447, 939
TscAI CASTG 2 cut(s) 354, 1092
TseFI GTSAC 1 cut(s) 1087
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 2 cut(s) 560, 1008
TspGWI ACGGA 2 cut(s) 174, 1171
TspRI CASTG 2 cut(s) 354, 1092
VpaK11BI GGWCC 5 cut(s) 281, 428, 761, 1160, 1169
XapI RAATTY 1 cut(s) 828
XceI RCATGY 1 cut(s) 803
XmiI GTMKAC 2 cut(s) 115, 384
XspI CTAG 2 cut(s) 416, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.