RLG00000015288

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004190
Physical Location & Seq
Forward (+)
64065686 .. 64068423
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015288

Sequence Viewer

Length: 1077 bp
ATGGTTTTCAATGTGATGCATTGTGGAGCTGTTGAAGGAGGAAGAACTGATAATAGCCAGTTAATATTTTGGGATGCATTTTTGAAAGCATGGAGTGAAGCATGTAAATGGGATGGAAGAGCAAAGGTTGAGATTCCGGCAGGAACATTCAAGTTGTTCCCAGTTGGAACTTTGCTAGCTTCTACTAGTCCATCAGCATTGAGTTCTAAAAATTGGATAAACTTTCGGAACATTGAACAACTGATCGTAGCCGGAGGCAGTACGCTGGATGGCCTGGGAGCCTCTGTTTGGTCTCTTAACGATTGCAAAACTAATCCCGGATGCAAAGCTCTCCCAATTTCGATGACTTTCAACTTCGTAACAAATGCAAGTGTTCATCACTTGAGATCATTCAACAGCCAAAATGGTCATTTCAAGGTTTATGGATGCCAGAAGATTAGAATATCAGCTCCTGAGGACAGAATTGTGTCGGGAAGTCAACAAATCCACATTTCTAATGTCTTTTGCAGTCCCGGACATGGAATTAGCATAGGAAGTCTTAGAGGGTATAGCTATACAGAATATGATGTGGAGGGAATTTTTGTGAAAAATAGCGTTTTCAAAAAGACTGATAACTATTTGAGAATCAATATATGGGCTCGGGATTCTCCGAAACCCTTGAAGGCTTCAAATATTGTGTACGAAGATATTAAAATGATTGATGTCATGAATCCCATTATCATCGATCAACAATATTGTCCAAACAATCAATGCAATCAACAGGCTGCTTCATCAAATGTGCAAATCAGCGATGTTACATTCAGAAACATATGGCGACTCTTCCTAAGATGCAGTGACTCTGAAATGCAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATCAAAAAACCTCAACTCAGCCCACACCAACTGGCACCACCACCAGAGCTTTCAATGCTATCATCAACCGTCTCTCATCTCAAGGCTCTCACCATGAAGTCCTTGTTACATACCACTCTATGCTCAAAACCCACATAGCTCTGGATATCCACACCTTCCCTAATCTGCTCAAAGCTTGCACCTTATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

359

Amino Acids

40.03

Weight (kDa)

8.61

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 149 9.3e-15 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 155 - 270 2.5e-31 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 920
AcsI RAATTY 1 cut(s) 576
AfaI GTAC 2 cut(s) 262, 680
AfiI CCNNNNNNNGG 2 cut(s) 288, 518
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 273
AluBI AGCT 8 cut(s) 29, 179, 329, 449, 552, 935, 1025, 1061
AluI AGCT 8 cut(s) 29, 179, 329, 449, 552, 935, 1025, 1061
Alw26I GTCTC 2 cut(s) 297, 962
AlwNI CAGNNNCTG 1 cut(s) 452
Ama87I CYCGRG 1 cut(s) 639
AoxI GGCC 1 cut(s) 271
ApeKI GCWGC 2 cut(s) 764, 847
ApoI RAATTY 1 cut(s) 576
AsuC2I CCSGG 2 cut(s) 318, 513
AsuHPI GGTGA 1 cut(s) 968
AsuNHI GCTAGC 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 639
AxyI CCTNAGG 1 cut(s) 453
BanI GGYRCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 640
BbvI GCAGC 2 cut(s) 751, 859
BccI CCATC 4 cut(s) 107, 199, 263, 872
BciT130I CCWGG 1 cut(s) 275
BcnI CCSGG 2 cut(s) 318, 513
BcoDI GTCTC 2 cut(s) 297, 962
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 2 cut(s) 176, 186
BisI GCNGC 2 cut(s) 765, 848
BlsI GCNGC 2 cut(s) 766, 849
Bme1390I CCNGG 3 cut(s) 275, 318, 513
BmeT110I CYCGRG 1 cut(s) 639
BmiI GGNNCC 2 cut(s) 280, 922
BmrFI CCNGG 3 cut(s) 275, 318, 513
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 5 cut(s) 6, 64, 311, 416, 818
BmtI GCTAGC 1 cut(s) 179
BmuI ACTGGG 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 403, 951
BpuMI CCSGG 2 cut(s) 318, 513
Bsa29I ATCGAT 1 cut(s) 723
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 270, 300
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 518
Bse1I ACTGG 3 cut(s) 58, 161, 922
Bse21I CCTNAGG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 5 cut(s) 79, 118, 274, 326, 431
BseLI CCNNNNNNNGG 2 cut(s) 288, 518
BseMII CTCAG 2 cut(s) 444, 917
BseNI ACTGG 3 cut(s) 58, 161, 922
BseXI GCAGC 2 cut(s) 751, 859
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 1 cut(s) 920
BshVI ATCGAT 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 639
BsiSI CCGG 4 cut(s) 137, 252, 318, 513
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 2 cut(s) 288, 518
BsmAI GTCTC 2 cut(s) 297, 962
BsmBI CGTCTC 1 cut(s) 962
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 1 cut(s) 273
Bso31I GGTCTC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 639
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 3 cut(s) 243, 386, 724
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 2 cut(s) 445, 916
BspDI ATCGAT 1 cut(s) 723
BspHI TCATGA 1 cut(s) 705
BspLI GGNNCC 2 cut(s) 280, 922
BspOI GCTAGC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 920
BspTNI GGTCTC 1 cut(s) 297
BsrI ACTGG 3 cut(s) 58, 161, 922
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 3 cut(s) 243, 386, 724
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 956
Bst6I CTCTTC 2 cut(s) 112, 824
BstAPI GCANNNNNTGC 1 cut(s) 862
BstC8I GCNNGC 3 cut(s) 177, 858, 1063
BstDEI CTNAG 4 cut(s) 453, 539, 824, 903
BstF5I GGATG 5 cut(s) 79, 118, 274, 326, 431
BstKTI GATC 3 cut(s) 246, 389, 727
BstMAI GTCTC 2 cut(s) 297, 962
BstMBI GATC 3 cut(s) 243, 386, 724
BstMWI GCNNNNNNNGC 3 cut(s) 853, 862, 941
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 3 cut(s) 273, 316, 511
BstV1I GCAGC 2 cut(s) 751, 859
Bsu15I ATCGAT 1 cut(s) 723
Bsu36I CCTNAGG 1 cut(s) 453
BsuRI GGCC 1 cut(s) 273
BsuTUI ATCGAT 1 cut(s) 723
BtgZI GCGATG 1 cut(s) 804
BtsCI GGATG 5 cut(s) 79, 118, 274, 326, 431
BtsI GCAGTG 1 cut(s) 838
BtsIMutI CAGTG 1 cut(s) 838
Cac8I GCNNGC 3 cut(s) 177, 858, 1063
CaiI CAGNNNCTG 1 cut(s) 452
CciI TCATGA 1 cut(s) 705
ClaI ATCGAT 1 cut(s) 723
Csp6I GTAC 2 cut(s) 261, 679
CviAII CATG 6 cut(s) 90, 102, 518, 706, 862, 980
CviQI GTAC 2 cut(s) 261, 679
DdeI CTNAG 4 cut(s) 453, 539, 824, 903
DpnI GATC 3 cut(s) 245, 388, 726
DpnII GATC 3 cut(s) 243, 386, 724
Eam1104I CTCTTC 2 cut(s) 112, 824
EarI CTCTTC 2 cut(s) 112, 824
Eco24I GRGCYC 1 cut(s) 640
Eco31I GGTCTC 1 cut(s) 297
Eco32I GATATC 1 cut(s) 1033
Eco81I CCTNAGG 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 273
EcoRV GATATC 1 cut(s) 1033
EcoT22I ATGCAT 2 cut(s) 21, 79
EcoT38I GRGCYC 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 962
FaeI CATG 6 cut(s) 93, 105, 521, 709, 865, 983
FaqI GGGAC 1 cut(s) 495
FatI CATG 6 cut(s) 89, 101, 517, 705, 861, 979
FauNDI CATATG 1 cut(s) 809
Fnu4HI GCNGC 2 cut(s) 765, 848
FokI GGATG 5 cut(s) 86, 125, 281, 333, 438
FriOI GRGCYC 1 cut(s) 640
Fsp4HI GCNGC 2 cut(s) 765, 848
FspBI CTAG 2 cut(s) 176, 186
GluI GCNGC 2 cut(s) 765, 848
HaeIII GGCC 1 cut(s) 273
HapII CCGG 4 cut(s) 137, 252, 318, 513
Hin1II CATG 6 cut(s) 93, 105, 521, 709, 865, 983
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 6 cut(s) 133, 624, 644, 709, 816, 836
HpaII CCGG 4 cut(s) 137, 252, 318, 513
HphI GGTGA 1 cut(s) 968
Hpy166II GTNNAC 2 cut(s) 479, 679
Hpy188I TCNGA 4 cut(s) 228, 651, 803, 841
Hpy188III TCNNGA 5 cut(s) 452, 471, 641, 706, 1028
Hpy8I GTNNAC 2 cut(s) 479, 679
HpyAV CCTTC 3 cut(s) 29, 655, 1051
HpyCH4III ACNGT 1 cut(s) 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 853, 862, 941
HpyF3I CTNAG 4 cut(s) 453, 539, 824, 903
Hsp92II CATG 6 cut(s) 93, 105, 521, 709, 865, 983
Kzo9I GATC 3 cut(s) 243, 386, 724
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 3 cut(s) 26, 278, 454
Lsp1109I GCAGC 2 cut(s) 751, 859
LweI GCATC 5 cut(s) 6, 64, 311, 416, 818
MaeI CTAG 2 cut(s) 176, 186
MaeIII GTNAC 4 cut(s) 358, 793, 833, 991
MalI GATC 3 cut(s) 245, 388, 726
MboI GATC 3 cut(s) 243, 386, 724
MboII GAAGA 5 cut(s) 54, 129, 445, 695, 811
MhlI GDGCHC 1 cut(s) 640
MluCI AATT 5 cut(s) 211, 336, 462, 522, 576
MlyI GAGTC 2 cut(s) 810, 830
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 7 cut(s) 32, 248, 292, 448, 536, 565, 907
Mph1103I ATGCAT 2 cut(s) 21, 79
MseI TTAA 3 cut(s) 62, 297, 690
MslI CAYNNNNRTG 1 cut(s) 106
MspI CCGG 4 cut(s) 137, 252, 318, 513
MspR9I CCNGG 3 cut(s) 275, 318, 513
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 853, 862, 941
NciI CCSGG 2 cut(s) 318, 513
NdeI CATATG 1 cut(s) 809
NdeII GATC 3 cut(s) 243, 386, 724
NheI GCTAGC 1 cut(s) 175
NlaIII CATG 6 cut(s) 93, 105, 521, 709, 865, 983
NlaIV GGNNCC 2 cut(s) 280, 922
NmuCI GTSAC 1 cut(s) 833
NsiI ATGCAT 2 cut(s) 21, 79
NspI RCATGY 1 cut(s) 105
PagI TCATGA 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 112
PfeI GAWTC 4 cut(s) 133, 624, 644, 709
PfoI TCCNGGA 2 cut(s) 316, 511
PkrI GCNGC 2 cut(s) 766, 849
PleI GAGTC 2 cut(s) 810, 830
PpsI GAGTC 2 cut(s) 810, 830
Psp6I CCWGG 1 cut(s) 273
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 280, 922
PstNI CAGNNNCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 262, 680
RsaNI GTAC 2 cut(s) 261, 679
RseI CAYNNNNRTG 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 3 cut(s) 62, 297, 690
SatI GCNGC 2 cut(s) 765, 848
Sau3AI GATC 3 cut(s) 243, 386, 724
SchI GAGTC 2 cut(s) 810, 830
ScrFI CCNGG 3 cut(s) 275, 318, 513
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 5 cut(s) 6, 64, 311, 416, 818
SmiMI CAYNNNNRTG 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 382, 966
SmoI CTYRAG 2 cut(s) 382, 966
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 5 cut(s) 211, 336, 462, 522, 576
SspI AATATT 3 cut(s) 66, 673, 734
SspMI CTAG 2 cut(s) 176, 186
StyD4I CCNGG 3 cut(s) 273, 316, 511
TaaI ACNGT 1 cut(s) 956
TaqI TCGA 2 cut(s) 341, 723
TasI AATT 5 cut(s) 211, 336, 462, 522, 576
TfiI GAWTC 4 cut(s) 133, 624, 644, 709
Tru1I TTAA 3 cut(s) 62, 297, 690
Tru9I TTAA 3 cut(s) 62, 297, 690
TscAI CASTG 1 cut(s) 838
TseFI GTSAC 1 cut(s) 833
TseI GCWGC 2 cut(s) 764, 847
Tsp45I GTSAC 1 cut(s) 833
TspDTI ATGAA 4 cut(s) 365, 722, 759, 996
TspRI CASTG 1 cut(s) 838
XapI RAATTY 1 cut(s) 576
XceI RCATGY 1 cut(s) 105
XspI CTAG 2 cut(s) 176, 186
Zsp2I ATGCAT 2 cut(s) 21, 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.