RLG00000033042

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004190
Physical Location & Seq
Forward (+)
22509743 .. 22512264
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033042

Sequence Viewer

Length: 1236 bp
ATGAGTTTGGTATCTTCATTCCTAGGGTTTAGGGTTTTCTTGTGTTTGTGCTTGTTGGTATGGGTAACAAAAGGCCAAAATGTCAACCTCTTTCGTCAAACCGAACAACTTGGTCGGCGCAAGATTTTCAATGTGATGGATTATGGAGCTGTTGAAGGTGGAATAACTGATAACAGCCAGGCATTTTTGAAAGCATGGAATGATGCCTGTAAATGGAATGGAAGGGCAAGGGTTTTGGTTCCCGGAGGAATATTCAAGTTATTCCCGGTTGTATTCTCAGGTCCATGCAATGGTCCGATTGCCTTTTTGATCATGGGGACTTTAAGAGCCTCTACTGATCCATCAACATTTAATTCTGAAAATTGGATAAACTTCCGATACTTGGAGCAGTTGACCGTAGCCGGAGGCGGTACCTTGGACGGCCAAGGATCCTTTGTTTGGCCTCTTAACAACTGCAACAATCGTCCATGCAAGACACTCCCCATCTCTTTGAGTTTTGACTTCGTCACAAATGCAAGCGTCGCTCACTTGAGATCATACAACAGCAAAAATGCCCATATCAAGGTTTATGGATGCAACAAAATGGACTTCACCAAACTTAGATTGTCAGCTCCAGGTGATAGCCCAAACACCGATGGCATAAAAATCGGAAACTCCTACCGCATCAGAATCTCACGCTCGGTTATTGGCACCGGGGATGACTGCATTGCGATATTGTCAGGAAGTAGGGAAATCCACATTTCTAAAATTTTTTGCGGTCCTGGGCATGGAATCAGCATTGGAAGTCTCGGAGGGTATGATAATGAATACGATGTGGAAGGAGTATATGTGAAAGATAGCGGTTTAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTTATTCTCATCCCTTGAAGGTTTCAAAGATTAGATACGAAAACATTGTGATGACTGATGTCACGAATCCAATTATTATCGATCAAAATTATTGCCCCAACCCTCCCTGCAATCAACAGGTCACTTCAAATGTGCAAATTAGTGATGTGACATATAATAACATATGGGGAAGTTCTTCTTCCAAAGAAGCAGTAATTCTGAAATGCAGCAAAACCAGCCCATGCAAGAACATAGTGATGAACAATATCAAGTTATCCCTCAATGGTCCTGGAGGTGTTGCGAGTTCTTCCTGCGAAAATGCCATTGGCGTCTCTTACGGCACACAAAACCCTCCTTCTTGCATAAATGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

412

Amino Acids

44.75

Weight (kDa)

9.02

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 395 5.6e-99 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 410, 689
AccB7I CCANNNNNTGG 1 cut(s) 290
AciI CCGC 4 cut(s) 408, 661, 756, 840
AclWI GGATC 3 cut(s) 332, 423, 436
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 1191
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 6 cut(s) 213, 290, 382, 438, 562, 767
AgsI TTSAA 7 cut(s) 130, 155, 190, 256, 901, 909, 1011
AjnI CCWGG 4 cut(s) 177, 613, 760, 1150
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 2 cut(s) 149, 611
AluI AGCT 2 cut(s) 149, 611
Alw26I GTCTC 2 cut(s) 791, 1198
AlwI GGATC 3 cut(s) 332, 423, 436
AoxI GGCC 3 cut(s) 73, 421, 440
ApeKI GCWGC 1 cut(s) 1089
ApoI RAATTY 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 1057
Asp718I GGTACC 1 cut(s) 410
AspA2I CCTAGG 1 cut(s) 22
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 4 cut(s) 281, 293, 758, 1148
AsuC2I CCSGG 3 cut(s) 243, 266, 694
AsuHPI GGTGA 2 cut(s) 583, 629
AvaII GGWCC 4 cut(s) 281, 293, 758, 1148
AvrII CCTAGG 1 cut(s) 22
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 2 cut(s) 410, 689
BbvI GCAGC 1 cut(s) 1101
BccI CCATC 4 cut(s) 130, 349, 491, 629
BceAI ACGGC 2 cut(s) 436, 1216
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 4 cut(s) 179, 615, 762, 1152
BclI TGATCA 1 cut(s) 309
BcnI CCSGG 3 cut(s) 243, 266, 694
BcoDI GTCTC 2 cut(s) 791, 1198
BfaI CTAG 2 cut(s) 23, 1234
BisI GCNGC 1 cut(s) 1090
BlnI CCTAGG 1 cut(s) 22
BlsI GCNGC 1 cut(s) 1091
Bme1390I CCNGG 7 cut(s) 179, 243, 266, 615, 694, 762, 1152
Bme18I GGWCC 4 cut(s) 281, 293, 758, 1148
BmgT120I GGNCC 4 cut(s) 281, 293, 758, 1148
BmiI GGNNCC 4 cut(s) 240, 412, 430, 691
BmrFI CCNGG 7 cut(s) 179, 243, 266, 615, 694, 762, 1152
BmsI GCATC 3 cut(s) 193, 563, 672
BoxI GACNNNNGTC 1 cut(s) 941
BpmI CTGGAG 2 cut(s) 597, 1173
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 3 cut(s) 243, 266, 694
Bsa29I ATCGAT 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 1191
BsaJI CCNNGG 5 cut(s) 22, 414, 424, 693, 761
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 290, 382, 438, 562, 767
Bse3DI GCAATG 2 cut(s) 295, 705
BseBI CCWGG 4 cut(s) 179, 615, 762, 1152
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 5 cut(s) 22, 414, 424, 693, 761
BseGI GGATG 3 cut(s) 578, 703, 892
BseLI CCNNNNNNNGG 6 cut(s) 213, 290, 382, 438, 562, 767
BseMI GCAATG 2 cut(s) 295, 705
BseMII CTCAG 1 cut(s) 291
BseXI GCAGC 1 cut(s) 1101
BshFI GGCC 3 cut(s) 75, 423, 442
BshNI GGYRCC 2 cut(s) 410, 689
BshVI ATCGAT 1 cut(s) 963
BsiSI CCGG 4 cut(s) 243, 266, 402, 693
BslFI GGGAC 2 cut(s) 331, 865
BslI CCNNNNNNNGG 6 cut(s) 213, 290, 382, 438, 562, 767
BsmAI GTCTC 2 cut(s) 791, 1198
BsmBI CGTCTC 1 cut(s) 1198
BsmFI GGGAC 2 cut(s) 331, 865
BsnI GGCC 3 cut(s) 75, 423, 442
Bsp143I GATC 5 cut(s) 309, 337, 428, 533, 964
BspACI CCGC 4 cut(s) 408, 661, 756, 840
BspANI GGCC 3 cut(s) 75, 423, 442
BspCNI CTCAG 1 cut(s) 290
BspDI ATCGAT 1 cut(s) 963
BspLI GGNNCC 4 cut(s) 240, 412, 430, 691
BspPI GGATC 3 cut(s) 332, 423, 436
BspT107I GGYRCC 2 cut(s) 410, 689
BsrDI GCAATG 2 cut(s) 295, 705
BssECI CCNNGG 5 cut(s) 22, 414, 424, 693, 761
BssMI GATC 5 cut(s) 309, 337, 428, 533, 964
BssNI GRCGYC 1 cut(s) 1191
BssT1I CCWWGG 3 cut(s) 22, 414, 424
Bst2UI CCWGG 4 cut(s) 179, 615, 762, 1152
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 1191
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 3 cut(s) 277, 599, 866
BstF5I GGATG 3 cut(s) 578, 703, 892
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 5 cut(s) 312, 340, 431, 536, 967
BstMAI GTCTC 2 cut(s) 791, 1198
BstMBI GATC 5 cut(s) 309, 337, 428, 533, 964
BstMWI GCNNNNNNNGC 2 cut(s) 521, 1098
BstNI CCWGG 4 cut(s) 179, 615, 762, 1152
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 7 cut(s) 177, 241, 264, 613, 692, 760, 1150
BstV1I GCAGC 1 cut(s) 1101
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
Bsu15I ATCGAT 1 cut(s) 963
BsuRI GGCC 3 cut(s) 75, 423, 442
BsuTUI ATCGAT 1 cut(s) 963
BtsCI GGATG 3 cut(s) 578, 703, 892
Cac8I GCNNGC 1 cut(s) 517
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 4 cut(s) 281, 293, 758, 1148
ClaI ATCGAT 1 cut(s) 963
CseI GACGC 2 cut(s) 508, 1180
Csp6I GTAC 1 cut(s) 411
CviAII CATG 7 cut(s) 195, 285, 313, 468, 767, 879, 1104
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 277, 599, 866
DpnI GATC 5 cut(s) 311, 339, 430, 535, 966
DpnII GATC 5 cut(s) 309, 337, 428, 533, 964
DraI TTTAAA 1 cut(s) 846
EaeI YGGCCR 1 cut(s) 421
Eco130I CCWWGG 3 cut(s) 22, 414, 424
Eco47I GGWCC 4 cut(s) 281, 293, 758, 1148
EcoRII CCWGG 4 cut(s) 177, 613, 760, 1150
EcoT14I CCWWGG 3 cut(s) 22, 414, 424
ErhI CCWWGG 3 cut(s) 22, 414, 424
Esp3I CGTCTC 1 cut(s) 1198
FaeI CATG 7 cut(s) 198, 288, 316, 471, 770, 882, 1107
FalI AAGNNNNNCTT 2 cut(s) 1045, 1077
FaqI GGGAC 2 cut(s) 331, 865
FatI CATG 7 cut(s) 194, 284, 312, 467, 766, 878, 1103
FauNDI CATATG 1 cut(s) 1046
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 1090
FokI GGATG 3 cut(s) 585, 710, 879
Fsp4HI GCNGC 1 cut(s) 1090
FspBI CTAG 2 cut(s) 23, 1234
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 1090
GsuI CTGGAG 2 cut(s) 597, 1173
HaeIII GGCC 3 cut(s) 75, 423, 442
HapII CCGG 4 cut(s) 243, 266, 402, 693
HgaI GACGC 2 cut(s) 508, 1180
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 1191
Hin1II CATG 7 cut(s) 198, 288, 316, 471, 770, 882, 1107
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 2 cut(s) 85, 393
HindII GTYRAC 2 cut(s) 85, 393
HinfI GANTC 4 cut(s) 669, 771, 870, 949
HpaII CCGG 4 cut(s) 243, 266, 402, 693
HphI GGTGA 2 cut(s) 583, 629
Hpy166II GTNNAC 2 cut(s) 85, 393
Hpy188I TCNGA 7 cut(s) 297, 358, 377, 650, 668, 791, 1083
Hpy188III TCNNGA 2 cut(s) 720, 946
Hpy8I GTNNAC 2 cut(s) 85, 393
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 5 cut(s) 149, 216, 812, 895, 1227
HpyCH4III ACNGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 1098
HpyF3I CTNAG 3 cut(s) 277, 599, 866
Hsp92I GRCGYC 1 cut(s) 1191
Hsp92II CATG 7 cut(s) 198, 288, 316, 471, 770, 882, 1107
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 5 cut(s) 309, 337, 428, 533, 964
LmnI GCTCC 3 cut(s) 146, 385, 616
Lsp1109I GCAGC 1 cut(s) 1101
LweI GCATC 3 cut(s) 193, 563, 672
MaeI CTAG 2 cut(s) 23, 1234
MaeIII GTNAC 5 cut(s) 64, 505, 943, 1003, 1030
MalI GATC 5 cut(s) 311, 339, 430, 535, 966
MboI GATC 5 cut(s) 309, 337, 428, 533, 964
MboII GAAGA 4 cut(s) 6, 1050, 1053, 1161
MflI RGATCY 1 cut(s) 428
MluCI AATT 7 cut(s) 352, 361, 747, 954, 970, 1020, 1077
MroXI GAANNNNTTC 1 cut(s) 1057
MseI TTAA 4 cut(s) 323, 351, 447, 845
MslI CAYNNNNRTG 2 cut(s) 932, 1118
MspI CCGG 4 cut(s) 243, 266, 402, 693
MspR9I CCNGG 7 cut(s) 179, 243, 266, 615, 694, 762, 1152
MvaI CCWGG 4 cut(s) 179, 615, 762, 1152
MwoI GCNNNNNNNGC 2 cut(s) 521, 1098
NciI CCSGG 3 cut(s) 243, 266, 694
NdeI CATATG 1 cut(s) 1046
NdeII GATC 5 cut(s) 309, 337, 428, 533, 964
NlaIII CATG 7 cut(s) 198, 288, 316, 471, 770, 882, 1107
NlaIV GGNNCC 4 cut(s) 240, 412, 430, 691
NmuCI GTSAC 4 cut(s) 505, 943, 1003, 1030
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 4 cut(s) 669, 771, 870, 949
PflFI GACNNNGTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 2 cut(s) 241, 1150
PkrI GCNGC 1 cut(s) 1091
PshAI GACNNNNGTC 1 cut(s) 941
Psp6I CCWGG 4 cut(s) 177, 613, 760, 1150
PspGI CCWGG 4 cut(s) 177, 613, 760, 1150
PspN4I GGNNCC 4 cut(s) 240, 412, 430, 691
PspPI GGNCC 4 cut(s) 281, 293, 758, 1148
PsuI RGATCY 1 cut(s) 428
PsyI GACNNNGTC 1 cut(s) 503
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 2 cut(s) 932, 1118
SaqAI TTAA 4 cut(s) 323, 351, 447, 845
SatI GCNGC 1 cut(s) 1090
Sau3AI GATC 5 cut(s) 309, 337, 428, 533, 964
Sau96I GGNCC 4 cut(s) 281, 293, 758, 1148
ScrFI CCNGG 7 cut(s) 179, 243, 266, 615, 694, 762, 1152
SfaNI GCATC 3 cut(s) 193, 563, 672
SinI GGWCC 4 cut(s) 281, 293, 758, 1148
SmiMI CAYNNNNRTG 2 cut(s) 932, 1118
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 7 cut(s) 352, 361, 747, 954, 970, 1020, 1077
SsiI CCGC 4 cut(s) 408, 661, 756, 840
SspI AATATT 1 cut(s) 252
SspMI CTAG 2 cut(s) 23, 1234
StyD4I CCNGG 7 cut(s) 177, 241, 264, 613, 692, 760, 1150
StyI CCWWGG 3 cut(s) 22, 414, 424
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 1 cut(s) 963
TasI AATT 7 cut(s) 352, 361, 747, 954, 970, 1020, 1077
TfiI GAWTC 4 cut(s) 669, 771, 870, 949
Tru1I TTAA 4 cut(s) 323, 351, 447, 845
Tru9I TTAA 4 cut(s) 323, 351, 447, 845
TseFI GTSAC 4 cut(s) 505, 943, 1003, 1030
TseI GCWGC 1 cut(s) 1089
Tsp45I GTSAC 4 cut(s) 505, 943, 1003, 1030
TspDTI ATGAA 3 cut(s) 6, 819, 1136
Tth111I GACNNNGTC 1 cut(s) 503
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 4 cut(s) 281, 293, 758, 1148
XapI RAATTY 1 cut(s) 747
XmaJI CCTAGG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 2 cut(s) 23, 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.