MD05G1208400.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004190
Physical Location & Seq
Reverse (-)
33973505 .. 33976591
3087 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1208400.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGGTATGGGTTTGATCATATCTTCTCTAGGTAGGTCCTTCTTGTTATTTTTCTCATTGGTATGGGTAACAGAAGCTCTGGATCAAGCCAAGTATTTCAATGTGAGGAATTATGGAGCTGTTGAAGGTGGAGAAGCTGATAACAGCAAGAATGTGGACCCTTCGCCTTTGGTCATCTCTTCCCCCGATGGAGGAGCTAATGCATTTCTGGAAGCATGGAAAGAAGCATGTCAATGGAACGGAAGGGCACGGGTTGTGGTTCCCAGAGGTACATTCAAGTTGTATCCAGTGATATTCTCAGGTCCATGCAATGGTCCGATCGCCTTTCTGATTGAGGGGACTTTGAGGGCTTCAATTGATCCATCAACATTTAGTACTGACAGTTGGATAAACTTCCGGTACATCAATCAGTTGGTAGTGACCGGAGGCGGTACCTTGGACGGCCAAGGAGCCTCGGCTTGGCATCTGAACAACTGCAAAACCAATCCCCAATGCCAGGCTCTTCCTATTTCTATGAGTTTTGACTTCATCACAAACGCAAGAGTTGAATACATAAAATCATACGACAGCAAGAATGCCCATTTCAAAATTTTCGGATGCCACAACATGGACTTCAGGAAAATCAAAGTCTCAGCTCCAGCAGACAGCCCGAACACCGATGGCATTAAGATCGGAAGCTCATACCGAATTAGAATCGCACGTTCCACAATCGCAACGGGCGATGACTGCGTAGCAGTGGTGACCGGAAGTAAGAAAATCCACATTTCTAAAGTTGTGTGCGGTCCCGGGCATGGAATCAGCATCGGAAGTCTAGGAGGGCATGACAAAGAAGACGCTGTGGTAGGAGTGGTGGTGAAAAACTGCACATTTTTGGGAACGGAGAATGGTGTGAGGATCAAAACTTGGGCTTCTCCTTATACCAGCAAGGCTTCGAATTTTACGTTCCAAGACATTTTTATGGATGATGTTCTGAATCCCATTACAATCGATCAAGAATATTGCCCCAACCCTCCTTGCCATCAACAGACTTCTTCGAACGTGCAAATCAGCGACGTTACATACAAAAACATATGGGGAACTTCCTCGTCGGAAGTAGCCGTAAGTCTCCGATGCAGCAAAAGCAGGCCATGCAAGAACGTAGTGCTGGACATCATCAAGTTGTCCCCTTCCAATCCCAGAGAACGTTTGAGTTCCTTCTGCTTTTATGCAAAAGGAGCCTCTTATGGCTTACAAGCCCCTCCATCTTGCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

418

Amino Acids

45.26

Weight (kDa)

8.57

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 77 - 391 9.6e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 431
AccB1I GGYRCC 1 cut(s) 431
AccB7I CCANNNNNTGG 2 cut(s) 311, 607
AciI CCGC 2 cut(s) 429, 780
AclI AACGTT 1 cut(s) 1183
AclWI GGATC 3 cut(s) 90, 353, 902
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 2 cut(s) 588, 934
AcuI CTGAAG 1 cut(s) 598
AfaI GTAC 4 cut(s) 271, 376, 401, 433
AfiI CCNNNNNNNGG 6 cut(s) 191, 311, 459, 496, 607, 791
AgsI TTSAA 6 cut(s) 100, 125, 277, 354, 548, 586
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 6 cut(s) 77, 119, 137, 197, 635, 678
AluI AGCT 6 cut(s) 77, 119, 137, 197, 635, 678
Alw26I GTCTC 2 cut(s) 634, 1109
AlwI GGATC 3 cut(s) 90, 353, 902
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 2 cut(s) 442, 1124
ApeKI GCWGC 1 cut(s) 1113
ApoI RAATTY 2 cut(s) 588, 934
Asp718I GGTACC 1 cut(s) 431
AspS9I GGNCC 5 cut(s) 36, 157, 302, 314, 782
AsuC2I CCSGG 2 cut(s) 786, 787
AsuHPI GGTGA 2 cut(s) 751, 865
AsuII TTCGAA 2 cut(s) 932, 1034
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 5 cut(s) 36, 157, 302, 314, 782
BaeGI GKGCMC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 837
BbvI GCAGC 1 cut(s) 1125
BccI CCATC 5 cut(s) 182, 370, 653, 1026, 1249
BceAI ACGGC 2 cut(s) 457, 1082
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 294
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 2 cut(s) 786, 787
BcoDI GTCTC 2 cut(s) 634, 1109
BfaI CTAG 2 cut(s) 29, 812
BfuI GTATCC 1 cut(s) 294
BisI GCNGC 1 cut(s) 1114
BlsI GCNGC 1 cut(s) 1115
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 3 cut(s) 497, 786, 787
Bme18I GGWCC 5 cut(s) 36, 157, 302, 314, 782
BmeT110I CYCGRG 1 cut(s) 785
BmgT120I GGNCC 5 cut(s) 36, 157, 302, 314, 782
BmiI GGNNCC 6 cut(s) 159, 261, 433, 451, 784, 1216
BmrFI CCNGG 3 cut(s) 497, 786, 787
BmsI GCATC 4 cut(s) 472, 587, 810, 1100
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 2 cut(s) 932, 1034
BpuMI CCSGG 2 cut(s) 786, 787
Bsa29I ATCGAT 1 cut(s) 987
BsaJI CCNNGG 4 cut(s) 435, 445, 453, 785
BsaWI WCCGGW 3 cut(s) 396, 422, 743
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 6 cut(s) 191, 311, 459, 496, 607, 791
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 316
BseBI CCWGG 1 cut(s) 497
BseCI ATCGAT 1 cut(s) 987
BseDI CCNNGG 4 cut(s) 435, 445, 453, 785
BseGI GGATG 2 cut(s) 602, 967
BseLI CCNNNNNNNGG 6 cut(s) 191, 311, 459, 496, 607, 791
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 312, 645
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 207
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 1125
BsgI GTGCAG 1 cut(s) 847
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 2 cut(s) 444, 1126
BshNI GGYRCC 1 cut(s) 431
BshVI ATCGAT 1 cut(s) 987
BsiEI CGRYCG 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 4 cut(s) 397, 423, 744, 786
BslFI GGGAC 3 cut(s) 352, 768, 1147
BslI CCNNNNNNNGG 6 cut(s) 191, 311, 459, 496, 607, 791
BsmAI GTCTC 2 cut(s) 634, 1109
BsmFI GGGAC 3 cut(s) 352, 768, 1147
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 444, 1126
BsoBI CYCGRG 1 cut(s) 785
Bsp119I TTCGAA 2 cut(s) 932, 1034
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
BspACI CCGC 2 cut(s) 429, 780
BspANI GGCC 2 cut(s) 444, 1126
BspCNI CTCAG 2 cut(s) 311, 644
BspDI ATCGAT 1 cut(s) 987
BspLI GGNNCC 6 cut(s) 159, 261, 433, 451, 784, 1216
BspPI GGATC 3 cut(s) 90, 353, 902
BspQI GCTCTTC 1 cut(s) 507
BspT104I TTCGAA 2 cut(s) 932, 1034
BspT107I GGYRCC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 4 cut(s) 435, 445, 453, 785
BssMI GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
BssT1I CCWWGG 2 cut(s) 435, 445
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 2 cut(s) 184, 507
BstAPI GCANNNNNTGC 1 cut(s) 1128
BstBI TTCGAA 2 cut(s) 932, 1034
BstC8I GCNNGC 1 cut(s) 1124
BstDEI CTNAG 2 cut(s) 298, 631
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 2 cut(s) 602, 967
BstKTI GATC 7 cut(s) 18, 85, 321, 361, 672, 897, 991
BstMAI GTCTC 2 cut(s) 634, 1109
BstMBI GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 4 cut(s) 726, 1119, 1128, 1214
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 1 cut(s) 231
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 3 cut(s) 495, 784, 785
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 1125
BstV2I GAAGAC 1 cut(s) 837
Bsu15I ATCGAT 1 cut(s) 987
BsuI GTATCC 1 cut(s) 294
BsuRI GGCC 2 cut(s) 444, 1126
BsuTUI ATCGAT 1 cut(s) 987
BtgZI GCGATG 1 cut(s) 735
BtsCI GGATG 2 cut(s) 602, 967
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 2 cut(s) 294, 741
Cac8I GCNNGC 1 cut(s) 1124
Cfr13I GGNCC 5 cut(s) 36, 157, 302, 314, 782
Cfr9I CCCGGG 1 cut(s) 785
ClaI ATCGAT 1 cut(s) 987
CseI GACGC 1 cut(s) 842
Csp6I GTAC 4 cut(s) 270, 375, 400, 432
CviAII CATG 7 cut(s) 216, 228, 306, 607, 791, 821, 1128
CviQI GTAC 4 cut(s) 270, 375, 400, 432
DdeI CTNAG 2 cut(s) 298, 631
DpnI GATC 7 cut(s) 17, 84, 320, 360, 671, 896, 990
DpnII GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
EaeI YGGCCR 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 184, 507
EarI CTCTTC 2 cut(s) 184, 507
Eco130I CCWWGG 2 cut(s) 435, 445
Eco47I GGWCC 5 cut(s) 36, 157, 302, 314, 782
Eco57I CTGAAG 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 785
Eco91I GGTNACC 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 36
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 1 cut(s) 495
EcoT14I CCWWGG 2 cut(s) 435, 445
EcoT22I ATGCAT 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 435, 445
FaeI CATG 7 cut(s) 219, 231, 309, 610, 794, 824, 1131
FaqI GGGAC 3 cut(s) 352, 768, 1147
FatI CATG 7 cut(s) 215, 227, 305, 606, 790, 820, 1127
FauNDI CATATG 1 cut(s) 1070
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 1114
FokI GGATG 2 cut(s) 609, 974
Fsp4HI GCNGC 1 cut(s) 1114
FspBI CTAG 2 cut(s) 29, 812
GluI GCNGC 1 cut(s) 1114
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 2 cut(s) 444, 1126
HapII CCGG 4 cut(s) 397, 423, 744, 786
HgaI GACGC 1 cut(s) 842
Hin1II CATG 7 cut(s) 219, 231, 309, 610, 794, 824, 1131
HinfI GANTC 3 cut(s) 693, 795, 973
HpaII CCGG 4 cut(s) 397, 423, 744, 786
HphI GGTGA 2 cut(s) 751, 865
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 9 cut(s) 318, 330, 468, 596, 674, 806, 972, 1090, 1109
Hpy188III TCNNGA 4 cut(s) 80, 209, 616, 992
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 2 cut(s) 1055, 1090
HpyAV CCTTC 6 cut(s) 49, 119, 171, 237, 1176, 1204
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4IV ACGT 6 cut(s) 700, 941, 1038, 1053, 1137, 1183
HpyCH4V TGCA 8 cut(s) 203, 309, 477, 864, 1042, 1113, 1131, 1208
HpyF10VI GCNNNNNNNGC 4 cut(s) 726, 1119, 1128, 1214
HpyF3I CTNAG 2 cut(s) 298, 631
HpySE526I ACGT 6 cut(s) 700, 941, 1038, 1053, 1137, 1183
Hsp92II CATG 7 cut(s) 219, 231, 309, 610, 794, 824, 1131
KpnI GGTACC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 5 cut(s) 116, 194, 449, 640, 1214
Lsp1109I GCAGC 1 cut(s) 1125
LweI GCATC 4 cut(s) 472, 587, 810, 1100
MaeI CTAG 2 cut(s) 29, 812
MaeII ACGT 6 cut(s) 700, 941, 1038, 1053, 1137, 1183
MaeIII GTNAC 4 cut(s) 67, 418, 739, 1054
MalI GATC 7 cut(s) 17, 84, 320, 360, 671, 896, 990
MboI GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
MboII GAAGA 5 cut(s) 15, 171, 494, 842, 1023
MfeI CAATTG 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 5 cut(s) 109, 354, 588, 687, 934
MmeI TCCRAC 2 cut(s) 365, 1068
Mph1103I ATGCAT 1 cut(s) 205
MseI TTAA 1 cut(s) 666
MslI CAYNNNNRTG 3 cut(s) 61, 232, 956
MspI CCGG 4 cut(s) 397, 423, 744, 786
MspR9I CCNGG 3 cut(s) 497, 786, 787
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 497
MwoI GCNNNNNNNGC 4 cut(s) 726, 1119, 1128, 1214
NciI CCSGG 2 cut(s) 786, 787
NdeI CATATG 1 cut(s) 1070
NdeII GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
NlaIII CATG 7 cut(s) 219, 231, 309, 610, 794, 824, 1131
NlaIV GGNNCC 6 cut(s) 159, 261, 433, 451, 784, 1216
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 2 cut(s) 418, 739
NsiI ATGCAT 1 cut(s) 205
NspI RCATGY 1 cut(s) 231
NspV TTCGAA 2 cut(s) 932, 1034
PciSI GCTCTTC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 2 cut(s) 717, 938
PctI GAATGC 1 cut(s) 580
PfeI GAWTC 3 cut(s) 693, 795, 973
PflMI CCANNNNNTGG 2 cut(s) 311, 607
PkrI GCNGC 1 cut(s) 1115
Ple19I CGATCG 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 36
Psp1406I AACGTT 1 cut(s) 1183
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 495
PspEI GGTNACC 1 cut(s) 739
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 6 cut(s) 159, 261, 433, 451, 784, 1216
PspPI GGNCC 5 cut(s) 36, 157, 302, 314, 782
PspPPI RGGWCCY 1 cut(s) 36
PvuI CGATCG 1 cut(s) 321
RsaI GTAC 4 cut(s) 271, 376, 401, 433
RsaNI GTAC 4 cut(s) 270, 375, 400, 432
RseI CAYNNNNRTG 3 cut(s) 61, 232, 956
SapI GCTCTTC 1 cut(s) 507
SaqAI TTAA 1 cut(s) 666
SatI GCNGC 1 cut(s) 1114
Sau3AI GATC 7 cut(s) 15, 82, 318, 358, 669, 894, 988
Sau96I GGNCC 5 cut(s) 36, 157, 302, 314, 782
ScaI AGTACT 1 cut(s) 376
ScrFI CCNGG 3 cut(s) 497, 786, 787
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 4 cut(s) 472, 587, 810, 1100
SfuI TTCGAA 2 cut(s) 932, 1034
SinI GGWCC 5 cut(s) 36, 157, 302, 314, 782
SmaI CCCGGG 1 cut(s) 787
SmiMI CAYNNNNRTG 3 cut(s) 61, 232, 956
Sse9I AATT 5 cut(s) 109, 354, 588, 687, 934
SsiI CCGC 2 cut(s) 429, 780
SspI AATATT 1 cut(s) 998
SspMI CTAG 2 cut(s) 29, 812
StyD4I CCNGG 3 cut(s) 495, 784, 785
StyI CCWWGG 2 cut(s) 435, 445
TaaI ACNGT 1 cut(s) 383
TaiI ACGT 6 cut(s) 703, 944, 1041, 1056, 1140, 1186
TaqI TCGA 3 cut(s) 932, 987, 1034
TasI AATT 5 cut(s) 109, 354, 588, 687, 934
TatI WGTACW 1 cut(s) 374
TfiI GAWTC 3 cut(s) 693, 795, 973
Tru1I TTAA 1 cut(s) 666
Tru9I TTAA 1 cut(s) 666
TscAI CASTG 2 cut(s) 294, 741
TseFI GTSAC 2 cut(s) 418, 739
TseI GCWGC 1 cut(s) 1113
Tsp45I GTSAC 2 cut(s) 418, 739
TspDTI ATGAA 1 cut(s) 517
TspGWI ACGGA 2 cut(s) 255, 893
TspMI CCCGGG 1 cut(s) 785
TspRI CASTG 2 cut(s) 294, 741
Van91I CCANNNNNTGG 2 cut(s) 311, 607
VpaK11BI GGWCC 5 cut(s) 36, 157, 302, 314, 782
XapI RAATTY 2 cut(s) 588, 934
XceI RCATGY 1 cut(s) 231
XmaI CCCGGG 1 cut(s) 785
XspI CTAG 2 cut(s) 29, 812
ZrmI AGTACT 1 cut(s) 376
Zsp2I ATGCAT 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.