FvH4_3g16670

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004190
Physical Location & Seq
Forward (+)
10498059 .. 10499825
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16670.t1

Sequence Viewer

Length: 1095 bp
ATGGATTATGGAGCTGTTGAAGGTGGAGTAACTGATAACAGCCAGGCATTCTTGAAAGCATGGAATGATGCATGTCAATGGAGAGGAATGGCAAGGGTTTTGATTCCCAGAGGAATATTCATGTTGTTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTCGGATCGCCTTTTTGATTCTGGGGACTTTAAGAGCCTCTACAGAACCATCAACATTCAACTCTGAGAATTGGATAAACTTCCGATACTTGGAGCAGTTAACCGTAGCCGGAGGCGGTACCTTGGACGGCCAAGGACCCTTAGTTTGGCCTCGTAACAACTGCAACAATCATCCATGCAGGACACTCCCCATCTCATTGAGTTTTGACTTCGTCAATAATGCAACCGTCGCATACTTGAGATCATACAACAGCAAAAATGCTCATATCAGGCTTTTTGGATGCAACAAAATGGACTTCACCAAACTTAGATTGTCAGCTCCAGGTGATAGCCCAAACACCGATGGCATAAAAATCGGAAGCTCCTACCGCATCAGAATCTCACGTTCGGTTATCACCACCGGCGATGACTGCATTGCCATATTGTCAGGAAGTAGGGAAATCCACATTCATAAGATTTTCTGCGGTCCTGGGCATGGAATCAGCATTGGAAGTCTCGGAGGGTATGGTAATGAACATGATGTTGAAGGAGTATATGTGAAAGATAGTGGTTCAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTGATTCTCATCCCTTGAAGGTTTCAAAGATTATATATGAAAACATTGTGATGACTGATGTCATGAATCCAATTATTATCGGTCAGAATTATTGCCCCAGCCCTCCCTGCAATCAACAGGTTACTTCGAATGTGCAGATTAGTAATGTGACATATAATAACATACGGGGAAGTTCTTCTTCCAAAGAAGCAGTGATTCTGAAATGCAGCAAAACCAGCCCTTGCAAGAACATAGTGATGAACAATATCAAGTTATCCCTCGACGGTCCTGGAGGTATTGCGAGTTCTTACTGTGAAAATGCCGTTGGCGTCACTTATGGCACACAAAACCCCCGTCCTTGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

365

Amino Acids

39.53

Weight (kDa)

8.99

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 25 - 350 8.8e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 275
AciI CCGC 3 cut(s) 273, 526, 621
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 1056
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 4 cut(s) 155, 247, 303, 632
AgsI TTSAA 7 cut(s) 20, 55, 217, 683, 711, 766, 774
AjnI CCWGG 4 cut(s) 42, 478, 625, 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AluBI AGCT 3 cut(s) 14, 476, 519
AluI AGCT 3 cut(s) 14, 476, 519
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 1 cut(s) 170
AoxI GGCC 2 cut(s) 286, 305
ApeKI GCWGC 1 cut(s) 954
ArsI GACNNNNNNTTYG 3 cut(s) 285, 317, 1066
Asp700I GAANNNNTTC 1 cut(s) 922
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 4 cut(s) 146, 293, 623, 1013
AsuHPI GGTGA 3 cut(s) 448, 494, 544
AsuII TTCGAA 1 cut(s) 875
AvaII GGWCC 4 cut(s) 146, 293, 623, 1013
BanI GGYRCC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 966
BccI CCATC 3 cut(s) 214, 356, 494
BceAI ACGGC 2 cut(s) 301, 1034
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 4 cut(s) 44, 480, 627, 1017
BcoDI GTCTC 1 cut(s) 656
BfmI CTRYAG 1 cut(s) 198
BisI GCNGC 1 cut(s) 955
BlsI GCNGC 1 cut(s) 956
Bme1390I CCNGG 4 cut(s) 44, 480, 627, 1017
Bme18I GGWCC 4 cut(s) 146, 293, 623, 1013
BmgT120I GGNCC 4 cut(s) 146, 293, 623, 1013
BmiI GGNNCC 2 cut(s) 277, 295
BmrFI CCNGG 4 cut(s) 44, 480, 627, 1017
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 3 cut(s) 58, 428, 537
BmuI ACTGGG 1 cut(s) 125
BoxI GACNNNNGTC 1 cut(s) 806
BpmI CTGGAG 2 cut(s) 462, 1038
Bpu14I TTCGAA 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 1056
BsaJI CCNNGG 3 cut(s) 279, 289, 626
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bsc4I CCNNNNNNNGG 4 cut(s) 155, 247, 303, 632
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 4 cut(s) 44, 480, 627, 1017
BseDI CCNNGG 3 cut(s) 279, 289, 626
BseGI GGATG 3 cut(s) 328, 443, 757
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 4 cut(s) 155, 247, 303, 632
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 2 cut(s) 156, 213
BseNI ACTGG 1 cut(s) 131
BseXI GCAGC 1 cut(s) 966
BseYI CCCAGC 1 cut(s) 845
BsgI GTGCAG 1 cut(s) 902
BshFI GGCC 2 cut(s) 288, 307
BshNI GGYRCC 1 cut(s) 275
BsiSI CCGG 2 cut(s) 267, 558
BslFI GGGAC 2 cut(s) 196, 730
BslI CCNNNNNNNGG 4 cut(s) 155, 247, 303, 632
BsmAI GTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 196, 730
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 2 cut(s) 288, 307
Bsp119I TTCGAA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 162, 398
BspACI CCGC 3 cut(s) 273, 526, 621
BspANI GGCC 2 cut(s) 288, 307
BspCNI CTCAG 2 cut(s) 155, 214
BspHI TCATGA 1 cut(s) 810
BspLI GGNNCC 2 cut(s) 277, 295
BspPI GGATC 1 cut(s) 170
BspT104I TTCGAA 1 cut(s) 875
BspT107I GGYRCC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 3 cut(s) 279, 289, 626
BssMI GATC 2 cut(s) 162, 398
BssNI GRCGYC 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 279, 289
Bst2UI CCWGG 4 cut(s) 44, 480, 627, 1017
Bst4CI ACNGT 4 cut(s) 262, 385, 1013, 1040
BstACI GRCGYC 1 cut(s) 1056
BstBI TTCGAA 1 cut(s) 875
BstDEI CTNAG 5 cut(s) 142, 222, 298, 464, 731
BstF5I GGATG 3 cut(s) 328, 443, 757
BstKTI GATC 2 cut(s) 165, 401
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 2 cut(s) 162, 398
BstMWI GCNNNNNNNGC 5 cut(s) 386, 525, 567, 855, 963
BstNI CCWGG 4 cut(s) 44, 480, 627, 1017
BstNSI RCATGY 1 cut(s) 75
BstPAI GACNNNNGTC 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 42, 478, 625, 1015
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 966
BsuRI GGCC 2 cut(s) 288, 307
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 3 cut(s) 328, 443, 757
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 1 cut(s) 945
CciI TCATGA 1 cut(s) 810
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 4 cut(s) 146, 293, 623, 1013
CseI GACGC 1 cut(s) 1045
Csp6I GTAC 1 cut(s) 276
CviAII CATG 9 cut(s) 60, 72, 121, 150, 333, 632, 674, 744, 811
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 5 cut(s) 142, 222, 298, 464, 731
DpnI GATC 2 cut(s) 164, 400
DpnII GATC 2 cut(s) 162, 398
EaeI YGGCCR 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 279, 289
Eco47I GGWCC 4 cut(s) 146, 293, 623, 1013
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 4 cut(s) 42, 478, 625, 1015
EcoT14I CCWWGG 2 cut(s) 279, 289
EcoT22I ATGCAT 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 279, 289
FaeI CATG 9 cut(s) 63, 75, 124, 153, 336, 635, 677, 747, 814
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 2 cut(s) 196, 730
FatI CATG 9 cut(s) 59, 71, 120, 149, 332, 631, 673, 743, 810
FauNDI CATATG 1 cut(s) 1091
Fnu4HI GCNGC 1 cut(s) 955
FokI GGATG 3 cut(s) 315, 450, 744
Fsp4HI GCNGC 1 cut(s) 955
GluI GCNGC 1 cut(s) 955
GsaI CCCAGC 1 cut(s) 849
GsuI CTGGAG 2 cut(s) 462, 1038
HaeIII GGCC 2 cut(s) 288, 307
HapII CCGG 2 cut(s) 267, 558
HgaI GACGC 1 cut(s) 1045
Hin1I GRCGYC 1 cut(s) 1056
Hin1II CATG 9 cut(s) 63, 75, 124, 153, 336, 635, 677, 747, 814
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 8 cut(s) 103, 175, 534, 636, 735, 752, 814, 943
HpaI GTTAAC 1 cut(s) 258
HpaII CCGG 2 cut(s) 267, 558
HphI GGTGA 3 cut(s) 448, 494, 544
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 8 cut(s) 162, 223, 242, 515, 533, 656, 834, 948
Hpy188III TCNNGA 3 cut(s) 52, 585, 811
Hpy8I GTNNAC 1 cut(s) 258
Hpy99I CGWCG 2 cut(s) 389, 1013
HpyAV CCTTC 3 cut(s) 14, 677, 760
HpyCH4III ACNGT 4 cut(s) 262, 385, 1013, 1040
HpyCH4IV ACGT 1 cut(s) 541
HpyF10VI GCNNNNNNNGC 5 cut(s) 386, 525, 567, 855, 963
HpyF3I CTNAG 5 cut(s) 142, 222, 298, 464, 731
HpySE526I ACGT 1 cut(s) 541
Hsp92I GRCGYC 1 cut(s) 1056
Hsp92II CATG 9 cut(s) 63, 75, 124, 153, 336, 635, 677, 747, 814
KpnI GGTACC 1 cut(s) 279
KspAI GTTAAC 1 cut(s) 258
Kzo9I GATC 2 cut(s) 162, 398
LmnI GCTCC 4 cut(s) 11, 250, 481, 524
Lsp1109I GCAGC 1 cut(s) 966
LweI GCATC 3 cut(s) 58, 428, 537
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 5 cut(s) 28, 311, 868, 895, 1057
MalI GATC 2 cut(s) 164, 400
MboI GATC 2 cut(s) 162, 398
MboII GAAGA 2 cut(s) 915, 918
MluCI AATT 3 cut(s) 226, 819, 835
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 9 cut(s) 77, 104, 205, 263, 318, 650, 861, 1013, 1016
Mph1103I ATGCAT 1 cut(s) 73
MroXI GAANNNNTTC 1 cut(s) 922
MseI TTAA 2 cut(s) 188, 257
MslI CAYNNNNRTG 3 cut(s) 76, 797, 983
MspI CCGG 2 cut(s) 267, 558
MspR9I CCNGG 4 cut(s) 44, 480, 627, 1017
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 4 cut(s) 44, 480, 627, 1017
MwoI GCNNNNNNNGC 5 cut(s) 386, 525, 567, 855, 963
NdeI CATATG 1 cut(s) 1091
NdeII GATC 2 cut(s) 162, 398
NlaIII CATG 9 cut(s) 63, 75, 124, 153, 336, 635, 677, 747, 814
NlaIV GGNNCC 2 cut(s) 277, 295
NmuCI GTSAC 2 cut(s) 895, 1057
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 1 cut(s) 75
NspV TTCGAA 1 cut(s) 875
PagI TCATGA 1 cut(s) 810
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 922
PfeI GAWTC 8 cut(s) 103, 175, 534, 636, 735, 752, 814, 943
PflFI GACNNNGTC 1 cut(s) 368
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 1 cut(s) 956
PpuMI RGGWCCY 1 cut(s) 293
PshAI GACNNNNGTC 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 42, 478, 625, 1015
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 4 cut(s) 42, 478, 625, 1015
PspN4I GGNNCC 2 cut(s) 277, 295
PspPI GGNCC 4 cut(s) 146, 293, 623, 1013
PspPPI RGGWCCY 1 cut(s) 293
PsrI GAACNNNNNNTAC 2 cut(s) 1015, 1047
PsyI GACNNNGTC 1 cut(s) 368
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 3 cut(s) 76, 797, 983
SaqAI TTAA 2 cut(s) 188, 257
SatI GCNGC 1 cut(s) 955
Sau3AI GATC 2 cut(s) 162, 398
Sau96I GGNCC 4 cut(s) 146, 293, 623, 1013
ScrFI CCNGG 4 cut(s) 44, 480, 627, 1017
SfaNI GCATC 3 cut(s) 58, 428, 537
SfcI CTRYAG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 875
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 4 cut(s) 146, 293, 623, 1013
SmiMI CAYNNNNRTG 3 cut(s) 76, 797, 983
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 3 cut(s) 226, 819, 835
SsiI CCGC 3 cut(s) 273, 526, 621
SspI AATATT 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 42, 478, 625, 1015
StyI CCWWGG 2 cut(s) 279, 289
TaaI ACNGT 4 cut(s) 262, 385, 1013, 1040
TaiI ACGT 1 cut(s) 544
TaqI TCGA 2 cut(s) 875, 1008
TaqII GACCGA 1 cut(s) 818
TasI AATT 3 cut(s) 226, 819, 835
TfiI GAWTC 8 cut(s) 103, 175, 534, 636, 735, 752, 814, 943
Tru1I TTAA 2 cut(s) 188, 257
Tru9I TTAA 2 cut(s) 188, 257
TscAI CASTG 1 cut(s) 945
TseFI GTSAC 2 cut(s) 895, 1057
TseI GCWGC 1 cut(s) 954
Tsp45I GTSAC 2 cut(s) 895, 1057
TspDTI ATGAA 6 cut(s) 109, 596, 684, 801, 827, 1001
TspRI CASTG 1 cut(s) 945
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 4 cut(s) 146, 293, 623, 1013
XceI RCATGY 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 922
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.