RLG00000031030

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004190
Physical Location & Seq
Reverse (-)
1476118 .. 1478134
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031030

Sequence Viewer

Length: 1248 bp
ATGATTGTTTTGATATCTTCCCTAGTCTTCAGGACTTTCTTGTTTTTGTTCTTGTTGGCATGGATAACAAGAGGCCACGATCTTGAACTCTATCGTCAAACAAAACATCTTGGTAGACCCATGGTTTTCAATGTGATGCGTTATGGAGCTATTGAAGGAGGAAGAACTGATAACAGTCAGGCATTTTTGAAAGCATGGAGTGAAGCATGTAAATGGGATGGAAGAGCAAAGGTTGAGATTCCGGCCGGAACATTCAAGTTGTTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTAACATGGCCTTTGTAATCAAGGGAACTTTGCTGGCTTCTACTAGTTCATCAGCATTGAGTTCTGAAAATTGGATAAACTTTCGGAACATTGAACAACTGACTGTAGCCGGGGGAGGTACGCTGGACGGTCAAGGAGCCTCGGTTTGGTCTCGTAACGATTGCAAAACTAATCCCGGATGCAAAGCTCTCCCAATTTCGATGACTTTTGACTTCGTAACAAATGCAAGTGTTCATCACTTGAGATCATTCAACAGCCAAAATGGTCATTTCAAGGTTTATGGAAGCCAGAAGATAGACTTCGGAAACATTAGAATATCGGCTCCTGAGGACAGTCCAAACACCGATGGCATAAAAATTGGAAACTCATACCGCATCAGAATTAGACGAACGATCATTGAGACCGGCGATGACTGTATAGGAATTGTATCGGGAAGTCAAAAGATCCACGTTTCTAATGTCTTTTGCGGTCCCGGACATGGAATTAGCATCGGAAGTCTTGGAGGGCATAGTTATACAGAATATGATGTGGAGGGAATTTTTGTGAAAAATAGCGTTTTCAAAAAGACTAAAAATGGTTTGAGAATCAAAACATGGGCTCGGGATTCTCCAAAACCATTGAAGGCTTCAAATATTGTGTATGAAGATATTAACATGATTGATGTCATGAATCCCATTGTCATCGATCAACAATATTGTCCGAACAATCAATGCAATCAACAGGCGTCTTCATCAAATGTGCAAATCAGCGATGTTACATTCAGAAACATATCGGGAACCTCGTCTTCCCAAGAAGCAGTGACTCTGAAATGCAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATCAAGTTACCTCATACTGATTCCAAAGGAGCTGCGACGACTTCCATCTGTAATAATGTCATCGGCAACTCTTATGGCTCACACATCCCTCCTAGTTGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

416

Amino Acids

45.44

Weight (kDa)

9.19

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 71 - 401 7.5e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 115
AciI CCGC 2 cut(s) 664, 759
AclWI GGATC 1 cut(s) 730
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 828
AcuI CTGAAG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 1016
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 3 cut(s) 290, 438, 770
AhlI ACTAGT 1 cut(s) 335
AluBI AGCT 3 cut(s) 149, 479, 1175
AluI AGCT 3 cut(s) 149, 479, 1175
Alw26I GTCTC 2 cut(s) 447, 686
AlwI GGATC 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 891
AoxI GGCC 3 cut(s) 73, 243, 300
ApeKI GCWGC 2 cut(s) 1104, 1175
ApoI RAATTY 1 cut(s) 828
AspS9I GGNCC 2 cut(s) 281, 761
AsuC2I CCSGG 3 cut(s) 403, 468, 765
AvaI CYCGRG 1 cut(s) 891
AvaII GGWCC 2 cut(s) 281, 761
AxyI CCTNAGG 1 cut(s) 618
BanII GRGCYC 1 cut(s) 892
BbsI GAAGAC 3 cut(s) 19, 1011, 1068
BbvI GCAGC 2 cut(s) 1116, 1162
BccI CCATC 4 cut(s) 212, 632, 1129, 1196
BcnI CCSGG 3 cut(s) 403, 468, 765
BcoDI GTCTC 2 cut(s) 447, 686
BcuI ACTAGT 1 cut(s) 335
BfaI CTAG 3 cut(s) 23, 336, 1236
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 2 cut(s) 1105, 1176
BlsI GCNGC 2 cut(s) 1106, 1177
Bme1390I CCNGG 3 cut(s) 403, 468, 765
Bme18I GGWCC 2 cut(s) 281, 761
BmeT110I CYCGRG 1 cut(s) 891
BmgT120I GGNCC 2 cut(s) 281, 761
BmiI GGNNCC 4 cut(s) 430, 615, 763, 1069
BmrFI CCNGG 3 cut(s) 403, 468, 765
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 4 cut(s) 126, 461, 675, 789
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 3 cut(s) 19, 1011, 1068
BpuEI CTTGAG 1 cut(s) 553
BpuMI CCSGG 3 cut(s) 403, 468, 765
Bsa29I ATCGAT 1 cut(s) 975
BsaHI GRCGYC 1 cut(s) 1016
BsaI GGTCTC 2 cut(s) 447, 686
BsaJI CCNNGG 3 cut(s) 120, 402, 432
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 438, 770
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 1 cut(s) 266
Bse21I CCTNAGG 1 cut(s) 618
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 3 cut(s) 120, 402, 432
BseGI GGATG 3 cut(s) 223, 476, 1227
BseLI CCNNNNNNNGG 3 cut(s) 290, 438, 770
BseMII CTCAG 2 cut(s) 291, 609
BseNI ACTGG 1 cut(s) 266
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 1116, 1162
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 75, 245, 302
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 891
BsiSI CCGG 6 cut(s) 242, 246, 402, 468, 696, 765
BslFI GGGAC 1 cut(s) 747
BslI CCNNNNNNNGG 3 cut(s) 290, 438, 770
BsmAI GTCTC 2 cut(s) 447, 686
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 3 cut(s) 75, 245, 302
Bso31I GGTCTC 2 cut(s) 447, 686
BsoBI CYCGRG 1 cut(s) 891
Bsp1286I GDGCHC 1 cut(s) 892
Bsp143I GATC 5 cut(s) 79, 536, 684, 735, 976
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 2 cut(s) 664, 759
BspANI GGCC 3 cut(s) 75, 245, 302
BspCNI CTCAG 2 cut(s) 290, 610
BspDI ATCGAT 1 cut(s) 975
BspHI TCATGA 1 cut(s) 957
BspLI GGNNCC 4 cut(s) 430, 615, 763, 1069
BspPI GGATC 1 cut(s) 730
BspQI GCTCTTC 1 cut(s) 217
BspTNI GGTCTC 2 cut(s) 447, 686
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 3 cut(s) 120, 402, 432
BssMI GATC 5 cut(s) 79, 536, 684, 735, 976
BssNI GRCGYC 1 cut(s) 1016
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 5 cut(s) 176, 397, 422, 626, 707
Bst6I CTCTTC 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 1016
BstAPI GCANNNNNTGC 1 cut(s) 1119
BstC8I GCNNGC 2 cut(s) 327, 1115
BstDEI CTNAG 2 cut(s) 277, 618
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 223, 476, 1227
BstKTI GATC 5 cut(s) 82, 539, 687, 738, 979
BstMAI GTCTC 2 cut(s) 447, 686
BstMBI GATC 5 cut(s) 79, 536, 684, 735, 976
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 1110, 1119
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 3 cut(s) 401, 466, 763
BstSFI CTRYAG 1 cut(s) 396
BstV1I GCAGC 2 cut(s) 1116, 1162
BstV2I GAAGAC 3 cut(s) 19, 1011, 1068
BstX2I RGATCY 1 cut(s) 735
BstYI RGATCY 1 cut(s) 735
BstZI CGGCCG 1 cut(s) 243
Bsu15I ATCGAT 1 cut(s) 975
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 3 cut(s) 75, 245, 302
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 2 cut(s) 714, 1056
BtsCI GGATG 3 cut(s) 223, 476, 1227
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 1 cut(s) 1095
Cac8I GCNNGC 2 cut(s) 327, 1115
CciI TCATGA 1 cut(s) 957
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 2 cut(s) 281, 761
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 1005
Csp6I GTAC 1 cut(s) 411
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 277, 618
DpnI GATC 5 cut(s) 81, 538, 686, 737, 978
DpnII GATC 5 cut(s) 79, 536, 684, 735, 976
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 892
Eco31I GGTCTC 2 cut(s) 447, 686
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 281, 761
Eco52I CGGCCG 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 13
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 891
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 892
ErhI CCWWGG 1 cut(s) 120
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 1 cut(s) 747
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 2 cut(s) 1105, 1176
FokI GGATG 3 cut(s) 230, 483, 1214
FriOI GRGCYC 1 cut(s) 892
Fsp4HI GCNGC 2 cut(s) 1105, 1176
FspBI CTAG 3 cut(s) 23, 336, 1236
GluI GCNGC 2 cut(s) 1105, 1176
HaeIII GGCC 3 cut(s) 75, 245, 302
HapII CCGG 6 cut(s) 242, 246, 402, 468, 696, 765
HgaI GACGC 1 cut(s) 1005
Hin1I GRCGYC 1 cut(s) 1016
HinfI GANTC 6 cut(s) 238, 876, 896, 961, 1093, 1163
HpaII CCGG 6 cut(s) 242, 246, 402, 468, 696, 765
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 8 cut(s) 358, 378, 596, 671, 785, 993, 1055, 1098
Hpy188III TCNNGA 7 cut(s) 31, 83, 617, 723, 893, 958, 1065
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 1 cut(s) 1183
HpyAV CCTTC 2 cut(s) 149, 907
HpyCH4III ACNGT 5 cut(s) 176, 397, 422, 626, 707
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 9 cut(s) 288, 456, 474, 518, 1005, 1033, 1104, 1122, 1242
HpyF10VI GCNNNNNNNGC 2 cut(s) 1110, 1119
HpyF3I CTNAG 2 cut(s) 277, 618
HpySE526I ACGT 1 cut(s) 741
Hsp92I GRCGYC 1 cut(s) 1016
Kzo9I GATC 5 cut(s) 79, 536, 684, 735, 976
LguI GCTCTTC 1 cut(s) 217
LmnI GCTCC 4 cut(s) 146, 428, 619, 1172
Lsp1109I GCAGC 2 cut(s) 1116, 1162
LweI GCATC 4 cut(s) 126, 461, 675, 789
MaeI CTAG 3 cut(s) 23, 336, 1236
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 6 cut(s) 293, 446, 508, 1045, 1090, 1149
MalI GATC 5 cut(s) 81, 538, 686, 737, 978
MboI GATC 5 cut(s) 79, 536, 684, 735, 976
MboII GAAGA 8 cut(s) 9, 19, 174, 234, 595, 947, 1011, 1068
MflI RGATCY 1 cut(s) 735
MhlI GDGCHC 1 cut(s) 892
MluCI AATT 7 cut(s) 361, 486, 648, 672, 714, 774, 828
MlyI GAGTC 1 cut(s) 1087
MseI TTAA 1 cut(s) 942
MslI CAYNNNNRTG 1 cut(s) 211
MspI CCGG 6 cut(s) 242, 246, 402, 468, 696, 765
MspR9I CCNGG 3 cut(s) 403, 468, 765
MwoI GCNNNNNNNGC 2 cut(s) 1110, 1119
NciI CCSGG 3 cut(s) 403, 468, 765
NcoI CCATGG 1 cut(s) 120
NdeII GATC 5 cut(s) 79, 536, 684, 735, 976
NlaIV GGNNCC 4 cut(s) 430, 615, 763, 1069
NmuCI GTSAC 1 cut(s) 1090
NspI RCATGY 1 cut(s) 210
PagI TCATGA 1 cut(s) 957
PciSI GCTCTTC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 1070
PfeI GAWTC 5 cut(s) 238, 876, 896, 961, 1163
PfoI TCCNGGA 2 cut(s) 466, 763
PkrI GCNGC 2 cut(s) 1106, 1177
PleI GAGTC 1 cut(s) 1087
PpsI GAGTC 1 cut(s) 1087
PspN4I GGNNCC 4 cut(s) 430, 615, 763, 1069
PspPI GGNCC 2 cut(s) 281, 761
PsuI RGATCY 1 cut(s) 735
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 1 cut(s) 211
SapI GCTCTTC 1 cut(s) 217
SaqAI TTAA 1 cut(s) 942
SatI GCNGC 2 cut(s) 1105, 1176
Sau3AI GATC 5 cut(s) 79, 536, 684, 735, 976
Sau96I GGNCC 2 cut(s) 281, 761
SchI GAGTC 1 cut(s) 1087
ScrFI CCNGG 3 cut(s) 403, 468, 765
SduI GDGCHC 1 cut(s) 892
SfaNI GCATC 4 cut(s) 126, 461, 675, 789
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 2 cut(s) 281, 761
SmiMI CAYNNNNRTG 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
SpeI ACTAGT 1 cut(s) 335
Sse9I AATT 7 cut(s) 361, 486, 648, 672, 714, 774, 828
SsiI CCGC 2 cut(s) 664, 759
SspI AATATT 2 cut(s) 925, 986
SspMI CTAG 3 cut(s) 23, 336, 1236
StyD4I CCNGG 3 cut(s) 401, 466, 763
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 5 cut(s) 176, 397, 422, 626, 707
TaiI ACGT 1 cut(s) 744
TaqI TCGA 2 cut(s) 491, 975
TasI AATT 7 cut(s) 361, 486, 648, 672, 714, 774, 828
TfiI GAWTC 5 cut(s) 238, 876, 896, 961, 1163
Tru1I TTAA 1 cut(s) 942
Tru9I TTAA 1 cut(s) 942
TscAI CASTG 1 cut(s) 1095
TseFI GTSAC 1 cut(s) 1090
TseI GCWGC 2 cut(s) 1104, 1175
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 5 cut(s) 330, 515, 948, 974, 1011
TspRI CASTG 1 cut(s) 1095
VpaK11BI GGWCC 2 cut(s) 281, 761
XapI RAATTY 1 cut(s) 828
XceI RCATGY 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 3 cut(s) 23, 336, 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.