Rroxscaffold_1G00051830

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004190
Physical Location & Seq
Reverse (-)
72608157 .. 72609600
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051830.1

Sequence Viewer

Length: 765 bp
ATGGACCAGCAAGCGTCTTTGAGTTTTGACTTCGTCACAAATGCAAGCGTCGCTCACTTGAGATCATACAACAGCAAAAATGCCCATATCAAGGTTTATGGATGCAACAAAATGGACTTCACCAAACTTAGCTTGTCAGCTCCAGGTGATAGCCCAAACACCGATGGCATAAAAATCGGAAACTCCTACCGCATCAGAATCTCACGCTCGGTTATTGACACCGGCGATGACTGCATTGCGATATTGTCAGGCAGTAGGGAAATCCACATTTCTAAAATTTTTTGCGGTCCTGGGCATGGAATCAGCATTGGAAGTCTCGGAGGGTATGATAATGAATACGATGTGGAAGGAGTATATGTGAAAGATAGCGGTTTAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTTATTCTCATCCCTTGAAGGTTTCAAAGATTAGATACGAAAACATTGTGATGACTGATGTCACGAATCCAATTATTATCGATCAAAATTATTGCCCCAGCCCTCCCTGCAATCAACAGGTTACTTCGAATGTGCAAATTAGTGATGTGACATATAATAACATATGGGGAAGTTCTTCTTCCAAAGAAGCAGTAATTCTGAAATGCAGCAAAACCAGCCCATGCAAGAACATAGTGATGAACAATATCAAGTTATCCCTCAATGGTCCTGGAGGTATTGCGAGTTCTTCCTGCGAAAATGCCATTGGCGTCTCTTACGGCACACAAAACCCTCCTTCTTGCATAAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

254

Amino Acids

27.37

Weight (kDa)

8.5

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 5 - 238 2.7e-78 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 190, 285, 369
AcsI RAATTY 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 91, 296
AgsI TTSAA 2 cut(s) 430, 438
AjnI CCWGG 3 cut(s) 142, 289, 679
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 2 cut(s) 132, 140
AluI AGCT 2 cut(s) 132, 140
Alw26I GTCTC 2 cut(s) 320, 727
ApeKI GCWGC 1 cut(s) 618
ApoI RAATTY 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 586
AspS9I GGNCC 3 cut(s) 4, 287, 677
AsuHPI GGTGA 2 cut(s) 112, 158
AsuII TTCGAA 1 cut(s) 539
AvaII GGWCC 3 cut(s) 4, 287, 677
BbvI GCAGC 1 cut(s) 630
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 745
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 3 cut(s) 144, 291, 681
BcoDI GTCTC 2 cut(s) 320, 727
BfaI CTAG 1 cut(s) 763
BisI GCNGC 1 cut(s) 619
BlsI GCNGC 1 cut(s) 620
Bme1390I CCNGG 3 cut(s) 144, 291, 681
Bme18I GGWCC 3 cut(s) 4, 287, 677
BmgT120I GGNCC 3 cut(s) 4, 287, 677
BmrFI CCNGG 3 cut(s) 144, 291, 681
BmsI GCATC 2 cut(s) 92, 201
BoxI GACNNNNGTC 1 cut(s) 470
BpmI CTGGAG 2 cut(s) 126, 702
Bpu14I TTCGAA 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 720
BsaJI CCNNGG 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 296
Bse118I RCCGGY 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 234
BseBI CCWGG 3 cut(s) 144, 291, 681
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 2 cut(s) 107, 421
BseLI CCNNNNNNNGG 2 cut(s) 91, 296
BseMI GCAATG 1 cut(s) 234
BseXI GCAGC 1 cut(s) 630
BseYI CCCAGC 1 cut(s) 509
BshVI ATCGAT 1 cut(s) 492
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 2 cut(s) 91, 296
BsmAI GTCTC 2 cut(s) 320, 727
BsmBI CGTCTC 1 cut(s) 727
BsmFI GGGAC 1 cut(s) 394
Bsp119I TTCGAA 1 cut(s) 539
Bsp143I GATC 2 cut(s) 62, 493
BspACI CCGC 3 cut(s) 190, 285, 369
BspDI ATCGAT 1 cut(s) 492
BspT104I TTCGAA 1 cut(s) 539
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 2 cut(s) 62, 493
BssNI GRCGYC 1 cut(s) 720
Bst2UI CCWGG 3 cut(s) 144, 291, 681
BstACI GRCGYC 1 cut(s) 720
BstBI TTCGAA 1 cut(s) 539
BstC8I GCNNGC 2 cut(s) 12, 46
BstDEI CTNAG 2 cut(s) 128, 395
BstF5I GGATG 2 cut(s) 107, 421
BstKTI GATC 2 cut(s) 65, 496
BstMAI GTCTC 2 cut(s) 320, 727
BstMBI GATC 2 cut(s) 62, 493
BstMWI GCNNNNNNNGC 4 cut(s) 50, 231, 519, 627
BstNI CCWGG 3 cut(s) 144, 291, 681
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 142, 289, 679
BstV1I GCAGC 1 cut(s) 630
Bsu15I ATCGAT 1 cut(s) 492
BsuTUI ATCGAT 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 107, 421
Cac8I GCNNGC 2 cut(s) 12, 46
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 3 cut(s) 4, 287, 677
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 3 cut(s) 3, 37, 709
CviAII CATG 3 cut(s) 296, 408, 633
CviJI RGCY 6 cut(s) 132, 140, 153, 413, 513, 630
CviKI_1 RGCY 6 cut(s) 132, 140, 153, 413, 513, 630
DdeI CTNAG 2 cut(s) 128, 395
DpnI GATC 2 cut(s) 64, 495
DpnII GATC 2 cut(s) 62, 493
DraI TTTAAA 1 cut(s) 375
Eco47I GGWCC 3 cut(s) 4, 287, 677
EcoRII CCWGG 3 cut(s) 142, 289, 679
Esp3I CGTCTC 1 cut(s) 727
FaeI CATG 3 cut(s) 299, 411, 636
FalI AAGNNNNNCTT 2 cut(s) 574, 606
FaqI GGGAC 1 cut(s) 394
FatI CATG 3 cut(s) 295, 407, 632
FauNDI CATATG 1 cut(s) 575
Fnu4HI GCNGC 1 cut(s) 619
FokI GGATG 2 cut(s) 114, 408
Fsp4HI GCNGC 1 cut(s) 619
FspBI CTAG 1 cut(s) 763
GluI GCNGC 1 cut(s) 619
GsaI CCCAGC 1 cut(s) 513
GsuI CTGGAG 2 cut(s) 126, 702
HapII CCGG 1 cut(s) 222
HgaI GACGC 3 cut(s) 3, 37, 709
Hin1I GRCGYC 1 cut(s) 720
Hin1II CATG 3 cut(s) 299, 411, 636
HinfI GANTC 4 cut(s) 198, 300, 399, 478
HpaII CCGG 1 cut(s) 222
HphI GGTGA 2 cut(s) 112, 158
Hpy188I TCNGA 4 cut(s) 179, 197, 320, 612
Hpy188III TCNNGA 1 cut(s) 475
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 3 cut(s) 341, 424, 756
HpyCH4V TGCA 8 cut(s) 44, 105, 234, 522, 547, 618, 636, 753
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 231, 519, 627
HpyF3I CTNAG 2 cut(s) 128, 395
Hsp92I GRCGYC 1 cut(s) 720
Hsp92II CATG 3 cut(s) 299, 411, 636
Kzo9I GATC 2 cut(s) 62, 493
LmnI GCTCC 1 cut(s) 145
Lsp1109I GCAGC 1 cut(s) 630
LweI GCATC 2 cut(s) 92, 201
MaeI CTAG 1 cut(s) 763
MaeIII GTNAC 4 cut(s) 34, 472, 532, 559
MalI GATC 2 cut(s) 64, 495
MboI GATC 2 cut(s) 62, 493
MboII GAAGA 3 cut(s) 579, 582, 690
MluCI AATT 5 cut(s) 276, 483, 499, 549, 606
MnlI CCTC 5 cut(s) 314, 525, 677, 680, 753
MroXI GAANNNNTTC 1 cut(s) 586
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 2 cut(s) 461, 647
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 3 cut(s) 144, 291, 681
MvaI CCWGG 3 cut(s) 144, 291, 681
MwoI GCNNNNNNNGC 4 cut(s) 50, 231, 519, 627
NdeI CATATG 1 cut(s) 575
NdeII GATC 2 cut(s) 62, 493
NlaIII CATG 3 cut(s) 299, 411, 636
NmuCI GTSAC 3 cut(s) 34, 472, 559
NspV TTCGAA 1 cut(s) 539
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 4 cut(s) 198, 300, 399, 478
PflFI GACNNNGTC 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 679
PkrI GCNGC 1 cut(s) 620
PshAI GACNNNNGTC 1 cut(s) 470
Psp6I CCWGG 3 cut(s) 142, 289, 679
PspFI CCCAGC 1 cut(s) 509
PspGI CCWGG 3 cut(s) 142, 289, 679
PspPI GGNCC 3 cut(s) 4, 287, 677
PsrI GAACNNNNNNTAC 2 cut(s) 679, 711
PsyI GACNNNGTC 1 cut(s) 32
RseI CAYNNNNRTG 2 cut(s) 461, 647
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 1 cut(s) 619
Sau3AI GATC 2 cut(s) 62, 493
Sau96I GGNCC 3 cut(s) 4, 287, 677
ScrFI CCNGG 3 cut(s) 144, 291, 681
SetI ASST 7 cut(s) 96, 134, 142, 148, 435, 534, 688
SfaNI GCATC 2 cut(s) 92, 201
SfuI TTCGAA 1 cut(s) 539
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 3 cut(s) 4, 287, 677
SmiMI CAYNNNNRTG 2 cut(s) 461, 647
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 5 cut(s) 276, 483, 499, 549, 606
SsiI CCGC 3 cut(s) 190, 285, 369
SspMI CTAG 1 cut(s) 763
StyD4I CCNGG 3 cut(s) 142, 289, 679
TaqI TCGA 2 cut(s) 492, 539
TasI AATT 5 cut(s) 276, 483, 499, 549, 606
TfiI GAWTC 4 cut(s) 198, 300, 399, 478
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TseFI GTSAC 3 cut(s) 34, 472, 559
TseI GCWGC 1 cut(s) 618
Tsp45I GTSAC 3 cut(s) 34, 472, 559
TspDTI ATGAA 2 cut(s) 348, 665
Tth111I GACNNNGTC 1 cut(s) 32
VpaK11BI GGWCC 3 cut(s) 4, 287, 677
XapI RAATTY 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.