Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0004190

Physical Location & Seq

Genomic Coordinates Reverse (-)

20054321 .. 20056143

Gene Structure 1823 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g16830.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGATATGGGTTCGATCATATATTCTCTAGGCAGGGTCTTCTTGTTATTCTTCTTGTTGGTATGGGAAACAGAAGGTCGTGATCAAGCCAAGTATTTCGACGTGAGGAAATATGGTGCTGTTGATGATGGAAAAACTGATAACAGCCAGGCATTTCTGGATGCATGGAAAGAAGCATGTCAATGGAAAGGAACGGCAAGGGTTGTGGTTCCCGGAGGAACATTCAAGTTGTATCCAGTGATATTCTCAGGTCCATGCAATGGTCCGATTGCCTTTCTGATTAAGGGTACTTTGAGTGCTACGACTAATCCATCAACATTTAGTGCTCACAGTTGGATAAACTTCCGGTACATCGATCAATTGACAGTGACCGGAGGCGGTACCTTGGACGGCCAAGGAGCCTCGGCTTGGCATCTTAACAACTGCAAAACCAATCCCCAATGCCAGGCTCTTCCTATTTCAATGAGTTTTGACTTCATCACAAACGCAAGAGTCGAATACATAAGATCATACGACAGCAAAAATGCACATTTCAAAATTTTCGGATGCCACAATATGGGCTTTAGGAAAATCAAAATCTCAGCTCCGGCTGACAGTCCGAACACCGATGGCATTAAGATTGGAAGCTCATACCGAATCAGAATCGCACGCTCTACGATCGCCACGGGTGATGACTGTGTCGCGATGGTGCACGGAAGTAGGAAAATCCACATTTCTAAAGTTGTGTGCGGTCCCGGGCATGGAATCAGCATCGGAAGCCTAGGAGGGAATGACAAAGAAGACGATGTGGTCGGAGTGACTGTGAAAAACTGCACCTTTTTGGGGACGGATAATGGTGTGAGGATCAAAACTTGGGCTTCTCCTTATACCAGTAAGGCTTCAAATTTTACATTCCAAGACATTTTCATGGATGACGTTCTGAATCCTATTACCATCGATCAAGAATATTGCCCCAACCCTCCTTGCAATCAACAGACTTCTTCGAATGTGCAAATCAGCGACGTTACGTACAAAAATATATGGGGAAGTTCGTCGTCGGAAGTAGCCGTCAGTTTCCGATGCAGCAAAACCTGCCCATGCAAGAACGTACTGCTGGACAGCATCAAAATGTCCCATTCTAATCCCAGAGGATGCTTGCGTTCCTTCTGCAAGTATGCCAAAGGCTCCTCTTATGGCTCACAAAGCCCTCCATCTTGTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

401

Amino Acids

43.73

Weight (kDa)

8.77

Isoelectric Point (pI)

31.06

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004190)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78400
fragaria_vesca	FvH4_3g01611 FvH4_3g01620 FvH4_3g16670 FvH4_6g48250
malus_domestica	MD05G1208400.v1.1 MD10G1194900.v1.1
prunus_persica	Prupe.4G149100_v2.0.a1
pyrus_communis	pycom10g16830
rosa_chinensis	RchiOBHm_Chr5g0002411 RchiOBHm_Chr5g0027771
rosa_laevigata	RLG00000015288 RLG00000031030 RLG00000033042
rosa_multiflora	Rmu_co8379139.1_g000001 Rmu_sc0000920.1_g000006
rosa_roxburghii	Rroxscaffold_1G00051830 Rroxscaffold_1G00051840 Rroxscaffold_1G00073440
rosa_rugosa	Rorug04G0396100 Rorug05G0101000 Rorug05G0517000
rosa_samantha	Rh5AG018900 Rh5AG195300 Rh5BG193600 Rh5CG020800 Rh5CG213900 Rh5DG019900 Rh5DG195600 Rh6CG028000 Rh6DG027300
rosa_wichuraiana	Rw5G001770 Rw5G017820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	788
Acc36I	ACCTGC	1 cut(s)	1079
Acc65I	GGTACC	1 cut(s)	380
AccB1I	GGYRCC	1 cut(s)	380
AccB7I	CCANNNNNTGG	2 cut(s)	260, 556
AccII	CGCG	1 cut(s)	683
AciI	CCGC	2 cut(s)	378, 729
AclWI	GGATC	1 cut(s)	851
AcoI	YGGCCR	1 cut(s)	391
AcsI	RAATTY	2 cut(s)	537, 883
AfaI	GTAC	5 cut(s)	289, 350, 382, 1010, 1089
AfiI	CCNNNNNNNGG	6 cut(s)	260, 408, 445, 556, 740, 822
AgsI	TTSAA	4 cut(s)	226, 462, 535, 882
AjiI	CACGTC	1 cut(s)	103
AjnI	CCWGG	2 cut(s)	147, 444
AluBI	AGCT	2 cut(s)	584, 627
AluI	AGCT	2 cut(s)	584, 627
Alw21I	GWGCWC	2 cut(s)	328, 693
Alw44I	GTGCAC	1 cut(s)	689
AlwI	GGATC	1 cut(s)	851
Ama87I	CYCGRG	1 cut(s)	734
AoxI	GGCC	1 cut(s)	391
ApaLI	GTGCAC	1 cut(s)	689
ApeKI	GCWGC	1 cut(s)	1062
ApoI	RAATTY	2 cut(s)	537, 883
Asp718I	GGTACC	1 cut(s)	380
AspA2I	CCTAGG	1 cut(s)	760
AspS9I	GGNCC	3 cut(s)	251, 263, 731
AsuC2I	CCSGG	3 cut(s)	213, 735, 736
AsuHPI	GGTGA	1 cut(s)	680
AsuII	TTCGAA	1 cut(s)	983
AvaI	CYCGRG	1 cut(s)	734
AvaII	GGWCC	3 cut(s)	251, 263, 731
AvrII	CCTAGG	1 cut(s)	760
BaeGI	GKGCMC	1 cut(s)	693
BanI	GGYRCC	1 cut(s)	380
BbsI	GAAGAC	2 cut(s)	31, 786
Bbv12I	GWGCWC	2 cut(s)	328, 693
BbvI	GCAGC	1 cut(s)	1074
BccI	CCATC	6 cut(s)	122, 319, 602, 679, 941, 1198
BceAI	ACGGC	3 cut(s)	210, 406, 1031
BciT130I	CCWGG	2 cut(s)	149, 446
BciVI	GTATCC	1 cut(s)	243
BclI	TGATCA	1 cut(s)	82
BcnI	CCSGG	3 cut(s)	213, 735, 736
BfaI	CTAG	2 cut(s)	29, 761
BfuAI	ACCTGC	1 cut(s)	1079
BfuI	GTATCC	1 cut(s)	243
BisI	GCNGC	1 cut(s)	1063
BlnI	CCTAGG	1 cut(s)	760
BlsI	GCNGC	1 cut(s)	1064
Bme1390I	CCNGG	5 cut(s)	149, 213, 446, 735, 736
Bme18I	GGWCC	3 cut(s)	251, 263, 731
BmeT110I	CYCGRG	1 cut(s)	734
BmgBI	CACGTC	1 cut(s)	103
BmgT120I	GGNCC	3 cut(s)	251, 263, 731
BmiI	GGNNCC	5 cut(s)	210, 382, 400, 733, 1165
BmrFI	CCNGG	5 cut(s)	149, 213, 446, 735, 736
BmsI	GCATC	7 cut(s)	151, 421, 536, 759, 1049, 1110, 1121
BpiI	GAAGAC	2 cut(s)	31, 786
Bpu14I	TTCGAA	1 cut(s)	983
BpuMI	CCSGG	3 cut(s)	213, 735, 736
Bsa29I	ATCGAT	2 cut(s)	354, 936
BsaAI	YACGTR	1 cut(s)	1008
BsaJI	CCNNGG	6 cut(s)	384, 394, 402, 663, 734, 760
BsaWI	WCCGGW	2 cut(s)	345, 371
BsaXI	ACNNNNNCTCC	2 cut(s)	786, 816
Bsc4I	CCNNNNNNNGG	6 cut(s)	260, 408, 445, 556, 740, 822
Bse1I	ACTGG	2 cut(s)	236, 870
Bse3DI	GCAATG	1 cut(s)	265
BseBI	CCWGG	2 cut(s)	149, 446
BseCI	ATCGAT	2 cut(s)	354, 936
BseDI	CCNNGG	6 cut(s)	384, 394, 402, 663, 734, 760
BseGI	GGATG	4 cut(s)	166, 551, 916, 1136
BseLI	CCNNNNNNNGG	6 cut(s)	260, 408, 445, 556, 740, 822
BseMI	GCAATG	1 cut(s)	265
BseMII	CTCAG	2 cut(s)	261, 594
BseNI	ACTGG	2 cut(s)	236, 870
BseRI	GAGGAG	1 cut(s)	1156
BseSI	GKGCMC	1 cut(s)	693
BseXI	GCAGC	1 cut(s)	1074
BsgI	GTGCAG	1 cut(s)	796
Bsh1236I	CGCG	1 cut(s)	683
Bsh1285I	CGRYCG	1 cut(s)	660
BshFI	GGCC	1 cut(s)	393
BshNI	GGYRCC	1 cut(s)	380
BshVI	ATCGAT	2 cut(s)	354, 936
BsiEI	CGRYCG	1 cut(s)	660
BsiHKAI	GWGCWC	2 cut(s)	328, 693
BsiHKCI	CYCGRG	1 cut(s)	734
BsiSI	CCGG	5 cut(s)	213, 346, 372, 587, 735
BslFI	GGGAC	3 cut(s)	717, 838, 1096
BslI	CCNNNNNNNGG	6 cut(s)	260, 408, 445, 556, 740, 822
BsmFI	GGGAC	3 cut(s)	717, 838, 1096
BsnI	GGCC	1 cut(s)	393
BsoBI	CYCGRG	1 cut(s)	734
Bsp119I	TTCGAA	1 cut(s)	983
Bsp1286I	GDGCHC	2 cut(s)	328, 693
Bsp143I	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
Bsp68I	TCGCGA	1 cut(s)	683
BspACI	CCGC	2 cut(s)	378, 729
BspANI	GGCC	1 cut(s)	393
BspCNI	CTCAG	2 cut(s)	260, 593
BspDI	ATCGAT	2 cut(s)	354, 936
BspFNI	CGCG	1 cut(s)	683
BspLI	GGNNCC	5 cut(s)	210, 382, 400, 733, 1165
BspMI	ACCTGC	1 cut(s)	1079
BspPI	GGATC	1 cut(s)	851
BspQI	GCTCTTC	1 cut(s)	456
BspT104I	TTCGAA	1 cut(s)	983
BspT107I	GGYRCC	1 cut(s)	380
BsrDI	GCAATG	1 cut(s)	265
BsrI	ACTGG	2 cut(s)	236, 870
BssECI	CCNNGG	6 cut(s)	384, 394, 402, 663, 734, 760
BssMI	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
BssT1I	CCWWGG	3 cut(s)	384, 394, 760
Bst2UI	CCWGG	2 cut(s)	149, 446
Bst4CI	ACNGT	5 cut(s)	332, 367, 596, 677, 802
Bst6I	CTCTTC	1 cut(s)	456
BstAPI	GCANNNNNTGC	1 cut(s)	1071
BstBAI	YACGTR	1 cut(s)	1008
BstBI	TTCGAA	1 cut(s)	983
BstC8I	GCNNGC	2 cut(s)	649, 1136
BstDEI	CTNAG	2 cut(s)	247, 580
BstDSI	CCRYGG	1 cut(s)	663
BstF5I	GGATG	4 cut(s)	166, 551, 916, 1136
BstFNI	CGCG	1 cut(s)	683
BstKTI	GATC	7 cut(s)	18, 85, 358, 509, 660, 846, 940
BstMBI	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
BstMCI	CGRYCG	1 cut(s)	660
BstMWI	GCNNNNNNNGC	3 cut(s)	756, 1071, 1182
BstNI	CCWGG	2 cut(s)	149, 446
BstNSI	RCATGY	1 cut(s)	180
BstSCI	CCNGG	5 cut(s)	147, 211, 444, 733, 734
BstSLI	GKGCMC	1 cut(s)	693
BstSNI	TACGTA	1 cut(s)	1008
BstUI	CGCG	1 cut(s)	683
BstV1I	GCAGC	1 cut(s)	1074
BstV2I	GAAGAC	2 cut(s)	31, 786
Bsu15I	ATCGAT	2 cut(s)	354, 936
BsuI	GTATCC	1 cut(s)	243
BsuRI	GGCC	1 cut(s)	393
BsuTUI	ATCGAT	2 cut(s)	354, 936
BtgI	CCRYGG	1 cut(s)	663
BtgZI	GCGATG	1 cut(s)	698
BtrI	CACGTC	1 cut(s)	103
BtsCI	GGATG	4 cut(s)	166, 551, 916, 1136
BtsIMutI	CAGTG	2 cut(s)	243, 372
BtuMI	TCGCGA	1 cut(s)	683
BveI	ACCTGC	1 cut(s)	1079
Cac8I	GCNNGC	2 cut(s)	649, 1136
Cfr13I	GGNCC	3 cut(s)	251, 263, 731
Cfr9I	CCCGGG	1 cut(s)	734
ClaI	ATCGAT	2 cut(s)	354, 936
Csp6I	GTAC	5 cut(s)	288, 349, 381, 1009, 1088
CspCI	CAANNNNNGTGG	2 cut(s)	186, 221
CviAII	CATG	6 cut(s)	165, 177, 255, 740, 907, 1077
CviQI	GTAC	5 cut(s)	288, 349, 381, 1009, 1088
DdeI	CTNAG	2 cut(s)	247, 580
DpnI	GATC	7 cut(s)	17, 84, 357, 508, 659, 845, 939
DpnII	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
DrdI	GACNNNNNNGTC	1 cut(s)	788
DseDI	GACNNNNNNGTC	1 cut(s)	788
EaeI	YGGCCR	1 cut(s)	391
Eam1104I	CTCTTC	1 cut(s)	456
EarI	CTCTTC	1 cut(s)	456
Eco105I	TACGTA	1 cut(s)	1008
Eco130I	CCWWGG	3 cut(s)	384, 394, 760
Eco47I	GGWCC	3 cut(s)	251, 263, 731
Eco88I	CYCGRG	1 cut(s)	734
EcoRII	CCWGG	2 cut(s)	147, 444
EcoT14I	CCWWGG	3 cut(s)	384, 394, 760
EcoT22I	ATGCAT	1 cut(s)	166
ErhI	CCWWGG	3 cut(s)	384, 394, 760
FaeI	CATG	6 cut(s)	168, 180, 258, 743, 910, 1080
FaqI	GGGAC	3 cut(s)	717, 838, 1096
FatI	CATG	6 cut(s)	164, 176, 254, 739, 906, 1076
FbaI	TGATCA	1 cut(s)	82
Fnu4HI	GCNGC	1 cut(s)	1063
FokI	GGATG	4 cut(s)	173, 558, 923, 1143
Fsp4HI	GCNGC	1 cut(s)	1063
FspBI	CTAG	2 cut(s)	29, 761
GluI	GCNGC	1 cut(s)	1063
HaeIII	GGCC	1 cut(s)	393
HapII	CCGG	5 cut(s)	213, 346, 372, 587, 735
Hin1II	CATG	6 cut(s)	168, 180, 258, 743, 910, 1080
HinfI	GANTC	5 cut(s)	492, 636, 642, 744, 922
HpaII	CCGG	5 cut(s)	213, 346, 372, 587, 735
HphI	GGTGA	1 cut(s)	680
Hpy166II	GTNNAC	1 cut(s)	691
Hpy188III	TCNNGA	4 cut(s)	80, 158, 682, 941
Hpy8I	GTNNAC	1 cut(s)	691
Hpy99I	CGWCG	4 cut(s)	104, 1004, 1036, 1039
HpyAV	CCTTC	2 cut(s)	68, 1153
HpyCH4III	ACNGT	5 cut(s)	332, 367, 596, 677, 802
HpyCH4IV	ACGT	5 cut(s)	102, 915, 1002, 1007, 1086
HpyF10VI	GCNNNNNNNGC	3 cut(s)	756, 1071, 1182
HpyF3I	CTNAG	2 cut(s)	247, 580
HpySE526I	ACGT	5 cut(s)	102, 915, 1002, 1007, 1086
Hsp92II	CATG	6 cut(s)	168, 180, 258, 743, 910, 1080
KpnI	GGTACC	1 cut(s)	384
Ksp22I	TGATCA	1 cut(s)	82
Kzo9I	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
LguI	GCTCTTC	1 cut(s)	456
LmnI	GCTCC	3 cut(s)	398, 589, 1169
Lsp1109I	GCAGC	1 cut(s)	1074
LweI	GCATC	7 cut(s)	151, 421, 536, 759, 1049, 1110, 1121
MaeI	CTAG	2 cut(s)	29, 761
MaeII	ACGT	5 cut(s)	102, 915, 1002, 1007, 1086
MaeIII	GTNAC	3 cut(s)	367, 796, 1003
MalI	GATC	7 cut(s)	17, 84, 357, 508, 659, 845, 939
MboI	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
MboII	GAAGA	5 cut(s)	31, 43, 443, 791, 972
MfeI	CAATTG	1 cut(s)	359
MhlI	GDGCHC	2 cut(s)	328, 693
MluCI	AATT	3 cut(s)	359, 537, 883
MlyI	GAGTC	1 cut(s)	501
MmeI	TCCRAC	3 cut(s)	314, 772, 1017
Mph1103I	ATGCAT	1 cut(s)	166
MseI	TTAA	3 cut(s)	282, 417, 615
MslI	CAYNNNNRTG	3 cut(s)	181, 905, 1106
MspI	CCGG	5 cut(s)	213, 346, 372, 587, 735
MspR9I	CCNGG	5 cut(s)	149, 213, 446, 735, 736
MunI	CAATTG	1 cut(s)	359
MvaI	CCWGG	2 cut(s)	149, 446
MvnI	CGCG	1 cut(s)	683
MwoI	GCNNNNNNNGC	3 cut(s)	756, 1071, 1182
NciI	CCSGG	3 cut(s)	213, 735, 736
NdeII	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
NlaIII	CATG	6 cut(s)	168, 180, 258, 743, 910, 1080
NlaIV	GGNNCC	5 cut(s)	210, 382, 400, 733, 1165
NmeAIII	GCCGAG	1 cut(s)	383
NmuCI	GTSAC	2 cut(s)	367, 796
NruI	TCGCGA	1 cut(s)	683
NsiI	ATGCAT	1 cut(s)	166
NspI	RCATGY	1 cut(s)	180
NspV	TTCGAA	1 cut(s)	983
PciSI	GCTCTTC	1 cut(s)	456
PcsI	WCGNNNNNNNCGW	1 cut(s)	492
PfeI	GAWTC	4 cut(s)	636, 642, 744, 922
PflFI	GACNNNGTC	1 cut(s)	677
PflMI	CCANNNNNTGG	2 cut(s)	260, 556
PfoI	TCCNGGA	1 cut(s)	211
PkrI	GCNGC	1 cut(s)	1064
Ple19I	CGATCG	1 cut(s)	660
PleI	GAGTC	1 cut(s)	500
PpsI	GAGTC	1 cut(s)	500
Ppu21I	YACGTR	1 cut(s)	1008
Psp6I	CCWGG	2 cut(s)	147, 444
PspGI	CCWGG	2 cut(s)	147, 444
PspN4I	GGNNCC	5 cut(s)	210, 382, 400, 733, 1165
PspPI	GGNCC	3 cut(s)	251, 263, 731
PsyI	GACNNNGTC	1 cut(s)	677
PvuI	CGATCG	1 cut(s)	660
RruI	TCGCGA	1 cut(s)	683
RsaI	GTAC	5 cut(s)	289, 350, 382, 1010, 1089
RsaNI	GTAC	5 cut(s)	288, 349, 381, 1009, 1088
RseI	CAYNNNNRTG	3 cut(s)	181, 905, 1106
SapI	GCTCTTC	1 cut(s)	456
SaqAI	TTAA	3 cut(s)	282, 417, 615
SatI	GCNGC	1 cut(s)	1063
Sau3AI	GATC	7 cut(s)	15, 82, 355, 506, 657, 843, 937
Sau96I	GGNCC	3 cut(s)	251, 263, 731
SchI	GAGTC	1 cut(s)	501
ScrFI	CCNGG	5 cut(s)	149, 213, 446, 735, 736
SduI	GDGCHC	2 cut(s)	328, 693
SfaNI	GCATC	7 cut(s)	151, 421, 536, 759, 1049, 1110, 1121
SfuI	TTCGAA	1 cut(s)	983
SinI	GGWCC	3 cut(s)	251, 263, 731
SmaI	CCCGGG	1 cut(s)	736
SmiMI	CAYNNNNRTG	3 cut(s)	181, 905, 1106
SnaBI	TACGTA	1 cut(s)	1008
Sse9I	AATT	3 cut(s)	359, 537, 883
SsiI	CCGC	2 cut(s)	378, 729
SspI	AATATT	1 cut(s)	947
SspMI	CTAG	2 cut(s)	29, 761
StyD4I	CCNGG	5 cut(s)	147, 211, 444, 733, 734
StyI	CCWWGG	3 cut(s)	384, 394, 760
TaaI	ACNGT	5 cut(s)	332, 367, 596, 677, 802
TaiI	ACGT	5 cut(s)	105, 918, 1005, 1010, 1089
TaqI	TCGA	6 cut(s)	14, 99, 354, 495, 936, 983
TasI	AATT	3 cut(s)	359, 537, 883
TfiI	GAWTC	4 cut(s)	636, 642, 744, 922
Tru1I	TTAA	3 cut(s)	282, 417, 615
Tru9I	TTAA	3 cut(s)	282, 417, 615
TscAI	CASTG	2 cut(s)	243, 372
TseFI	GTSAC	2 cut(s)	367, 796
TseI	GCWGC	1 cut(s)	1062
Tsp45I	GTSAC	2 cut(s)	367, 796
TspDTI	ATGAA	2 cut(s)	466, 895
TspGWI	ACGGA	2 cut(s)	708, 842
TspMI	CCCGGG	1 cut(s)	734
TspRI	CASTG	2 cut(s)	243, 372
Tth111I	GACNNNGTC	1 cut(s)	677
Van91I	CCANNNNNTGG	2 cut(s)	260, 556
VneI	GTGCAC	1 cut(s)	689
VpaK11BI	GGWCC	3 cut(s)	251, 263, 731
XapI	RAATTY	2 cut(s)	537, 883
XceI	RCATGY	1 cut(s)	180
XmaI	CCCGGG	1 cut(s)	734
XmaJI	CCTAGG	1 cut(s)	760
XspI	CTAG	2 cut(s)	29, 761
Zsp2I	ATGCAT	1 cut(s)	166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.