Rroxscaffold_1G00073440

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004190
Physical Location & Seq
Forward (+)
94306752 .. 94308666
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073440.1

Sequence Viewer

Length: 1044 bp
ATGGTTTTCAATGTGATGCGGTATGGAGCTATTGAAGGAGGAAGAACTGATAATAGTCAGGCATTTTTGAAAGCATGGAGTGACGCATGTAAATGGGATGGAAGAGCAAAGGTTGTGATTCCGGCCGGAACATTCAAGTTGTTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTAACACAGCCTTTGTAATCAAGGGAACTTTGTTGGCTTCTACTAGTTCATCAGCATTGAGTTCTGAAAATTGGATAAACTTTCGGAACATTGAACAATTGACTGTAGCGGGGGAGTCGATGACTTTCGACTTCGTAACAAATGCAAGTGTTCATCACTTGAGATCATTCAACAGCCAAAATGGTCATTTCAAGGTTCATGGATGCCAGAAGATAGACTTCGGAAACATTAGAATATCGGCTCCTAAGGATAGTCCAAACACCGATGGCATAAAAATTGGAAACTCATACCGCATCAGAATTAGACGAACGATCATTGAGACCGGCGATGATTGTATAGGAATTGTGTCGGGAAGTCAAAAAATCCACATTTCTAATGTCTTTTGCGGTCCCGGACATGGAATTAGCATCGGAAGTCTTGGAGGGCATAGTTATACAGAATATGATGTGGAGGGAATTTTTGTGAAAAATAGCGTTTTCAAAAAGACTGATAATGGTTTGAGAATCAAAACATGGGCTCGGGATTCTCTAAAACCATTGAAGGCTTCAAATATTGTGTATGAAGATATTAACATGATTGATGTCATGAATCCCATTGTCATCGATCAACAATATTGTCCGAACAATCAATGCAATCAACAGGCTTCTTCATCAAATGTGCAAATCAGCGATGTTACATTCAGAAACATATGGGGAACCTCGTCTTCCCAAGAAGCAGTGACTCTGAAATGCAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATCAAGTTACCTCATAATGGTTCCAAAGGAGCTGCGATTTCCATCTGTAATAATGTCATCGGCTACTCTTATGGCTCACACATCCCTCCTAGTTGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

347

Amino Acids

37.93

Weight (kDa)

9.05

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 3 - 194 3.3e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 97 - 333 4.2e-72 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 19, 281, 463, 558
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 1 cut(s) 627
AfiI CCNNNNNNNGG 3 cut(s) 170, 569, 959
AhlI ACTAGT 1 cut(s) 215
AluBI AGCT 2 cut(s) 29, 974
AluI AGCT 2 cut(s) 29, 974
Alw26I GTCTC 1 cut(s) 485
Ama87I CYCGRG 1 cut(s) 690
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 903, 974
ApoI RAATTY 1 cut(s) 627
AspS9I GGNCC 2 cut(s) 161, 560
AsuC2I CCSGG 1 cut(s) 564
AvaI CYCGRG 1 cut(s) 690
AvaII GGWCC 2 cut(s) 161, 560
AxyI CCTNAGG 1 cut(s) 417
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 867
BbvI GCAGC 2 cut(s) 915, 961
BccI CCATC 4 cut(s) 92, 431, 928, 992
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 1 cut(s) 485
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 2 cut(s) 216, 1032
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 904, 975
BlsI GCNGC 2 cut(s) 905, 976
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 2 cut(s) 161, 560
BmeT110I CYCGRG 1 cut(s) 690
BmgT120I GGNCC 2 cut(s) 161, 560
BmiI GGNNCC 4 cut(s) 414, 562, 868, 964
BmrFI CCNGG 1 cut(s) 564
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 4 cut(s) 6, 365, 474, 588
BmuI ACTGGG 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 867
BpuEI CTTGAG 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 564
Bsa29I ATCGAT 1 cut(s) 774
BsaBI GATNNNNATC 1 cut(s) 983
BsaI GGTCTC 1 cut(s) 485
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 569, 959
Bse118I RCCGGY 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 146
Bse21I CCTNAGG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 983
BseCI ATCGAT 1 cut(s) 774
BseGI GGATG 3 cut(s) 103, 380, 1023
BseJI GATNNNNATC 1 cut(s) 983
BseLI CCNNNNNNNGG 3 cut(s) 170, 569, 959
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 146
BseX3I CGGCCG 1 cut(s) 123
BseXI GCAGC 2 cut(s) 915, 961
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 125
BshVI ATCGAT 1 cut(s) 774
BsiEI CGRYCG 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 690
BsiSI CCGG 4 cut(s) 122, 126, 495, 564
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 3 cut(s) 170, 569, 959
BsmAI GTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 125
Bso31I GGTCTC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 691
Bsp143I GATC 3 cut(s) 335, 483, 775
BspACI CCGC 4 cut(s) 19, 281, 463, 558
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 170
BspDI ATCGAT 1 cut(s) 774
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 414, 562, 868, 964
BspQI GCTCTTC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 485
BsrFI RCCGGY 1 cut(s) 494
BsrI ACTGG 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 494
BssMI GATC 3 cut(s) 335, 483, 775
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 2 cut(s) 157, 417
BstF5I GGATG 3 cut(s) 103, 380, 1023
BstKTI GATC 3 cut(s) 338, 486, 778
BstMAI GTCTC 1 cut(s) 485
BstMBI GATC 3 cut(s) 335, 483, 775
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 909, 918
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 562
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 2 cut(s) 915, 961
BstV2I GAAGAC 1 cut(s) 867
BstZI CGGCCG 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 774
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 1 cut(s) 125
BsuTUI ATCGAT 1 cut(s) 774
BtgZI GCGATG 2 cut(s) 513, 855
BtsCI GGATG 3 cut(s) 103, 380, 1023
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 1 cut(s) 894
Cac8I GCNNGC 1 cut(s) 914
CciI TCATGA 1 cut(s) 756
Cfr10I RCCGGY 1 cut(s) 494
Cfr13I GGNCC 2 cut(s) 161, 560
ClaI ATCGAT 1 cut(s) 774
CseI GACGC 1 cut(s) 92
CviAII CATG 9 cut(s) 75, 87, 165, 371, 569, 684, 745, 757, 918
DdeI CTNAG 2 cut(s) 157, 417
DpnI GATC 3 cut(s) 337, 485, 777
DpnII GATC 3 cut(s) 335, 483, 775
EaeI YGGCCR 1 cut(s) 123
EagI CGGCCG 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 485
Eco47I GGWCC 2 cut(s) 161, 560
Eco52I CGGCCG 1 cut(s) 123
Eco81I CCTNAGG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 690
EcoT38I GRGCYC 1 cut(s) 691
FaeI CATG 9 cut(s) 78, 90, 168, 374, 572, 687, 748, 760, 921
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 546
FatI CATG 9 cut(s) 74, 86, 164, 370, 568, 683, 744, 756, 917
FauI CCCGC 1 cut(s) 274
FauNDI CATATG 1 cut(s) 860
Fnu4HI GCNGC 2 cut(s) 904, 975
FokI GGATG 3 cut(s) 110, 387, 1010
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 2 cut(s) 904, 975
FspBI CTAG 2 cut(s) 216, 1032
GluI GCNGC 2 cut(s) 904, 975
HaeIII GGCC 1 cut(s) 125
HapII CCGG 4 cut(s) 122, 126, 495, 564
HgaI GACGC 1 cut(s) 92
Hin1II CATG 9 cut(s) 78, 90, 168, 374, 572, 687, 748, 760, 921
HinfI GANTC 6 cut(s) 118, 287, 675, 695, 760, 892
HpaII CCGG 4 cut(s) 122, 126, 495, 564
Hpy188I TCNGA 8 cut(s) 238, 258, 395, 470, 584, 792, 854, 897
Hpy188III TCNNGA 3 cut(s) 522, 692, 757
HpyAV CCTTC 2 cut(s) 29, 706
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 6 cut(s) 168, 317, 804, 832, 903, 921
HpyF10VI GCNNNNNNNGC 2 cut(s) 909, 918
HpyF3I CTNAG 2 cut(s) 157, 417
Hsp92II CATG 9 cut(s) 78, 90, 168, 374, 572, 687, 748, 760, 921
Kzo9I GATC 3 cut(s) 335, 483, 775
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 3 cut(s) 26, 418, 971
LpnPI CCDG 9 cut(s) 44, 135, 139, 144, 159, 392, 508, 577, 797
Lsp1109I GCAGC 2 cut(s) 915, 961
LweI GCATC 4 cut(s) 6, 365, 474, 588
MaeI CTAG 2 cut(s) 216, 1032
MaeIII GTNAC 6 cut(s) 80, 173, 307, 844, 889, 948
MalI GATC 3 cut(s) 337, 485, 777
MboI GATC 3 cut(s) 335, 483, 775
MboII GAAGA 6 cut(s) 54, 114, 394, 746, 810, 867
MfeI CAATTG 1 cut(s) 269
MhlI GDGCHC 1 cut(s) 691
MluCI AATT 7 cut(s) 241, 269, 447, 471, 513, 573, 627
MlyI GAGTC 2 cut(s) 296, 886
MnlI CCTC 6 cut(s) 32, 587, 616, 880, 963, 1038
MseI TTAA 2 cut(s) 741, 1042
MslI CAYNNNNRTG 1 cut(s) 91
MspI CCGG 4 cut(s) 122, 126, 495, 564
MspR9I CCNGG 1 cut(s) 564
MunI CAATTG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 909, 918
NciI CCSGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 860
NdeII GATC 3 cut(s) 335, 483, 775
NlaIII CATG 9 cut(s) 78, 90, 168, 374, 572, 687, 748, 760, 921
NlaIV GGNNCC 4 cut(s) 414, 562, 868, 964
NmuCI GTSAC 2 cut(s) 80, 889
NspI RCATGY 1 cut(s) 90
PagI TCATGA 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 97
PfeI GAWTC 4 cut(s) 118, 675, 695, 760
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 2 cut(s) 905, 976
PleI GAGTC 2 cut(s) 295, 886
PpsI GAGTC 2 cut(s) 295, 886
PspN4I GGNNCC 4 cut(s) 414, 562, 868, 964
PspPI GGNCC 2 cut(s) 161, 560
RseI CAYNNNNRTG 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 741, 1042
SatI GCNGC 2 cut(s) 904, 975
Sau3AI GATC 3 cut(s) 335, 483, 775
Sau96I GGNCC 2 cut(s) 161, 560
SchI GAGTC 2 cut(s) 296, 886
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 691
SetI ASST 7 cut(s) 31, 114, 163, 369, 872, 955, 976
SfaNI GCATC 4 cut(s) 6, 365, 474, 588
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 2 cut(s) 161, 560
SmiMI CAYNNNNRTG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 7 cut(s) 241, 269, 447, 471, 513, 573, 627
SsiI CCGC 4 cut(s) 19, 281, 463, 558
SspI AATATT 2 cut(s) 724, 785
SspMI CTAG 2 cut(s) 216, 1032
StyD4I CCNGG 1 cut(s) 562
TaaI ACNGT 1 cut(s) 277
TaqI TCGA 3 cut(s) 290, 300, 774
TasI AATT 7 cut(s) 241, 269, 447, 471, 513, 573, 627
TfiI GAWTC 4 cut(s) 118, 675, 695, 760
Tru1I TTAA 2 cut(s) 741, 1042
Tru9I TTAA 2 cut(s) 741, 1042
TscAI CASTG 1 cut(s) 894
TseFI GTSAC 2 cut(s) 80, 889
TseI GCWGC 2 cut(s) 903, 974
Tsp45I GTSAC 2 cut(s) 80, 889
TspDTI ATGAA 6 cut(s) 210, 314, 359, 747, 773, 810
TspRI CASTG 1 cut(s) 894
VpaK11BI GGWCC 2 cut(s) 161, 560
XapI RAATTY 1 cut(s) 627
XceI RCATGY 1 cut(s) 90
XspI CTAG 2 cut(s) 216, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.