Rorug05G0101000

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004190
Physical Location & Seq
Reverse (-)
8902577 .. 8904238
1662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0101000.1

Sequence Viewer

Length: 1059 bp
ATGGAGGAGGAGGTGATTTCCAAGTTTGCAGGAAAAATTACACTCACTGAAGCTGAGCAGGAGGAAGTGATCCACGTCAAAACCCACGATTTTGAGTCCCTCAAGACCAAATTAGACCTATCTCTGGTAGGAAAGGTCCTCACTACAAATCCCTTCTGCAGAATCGCATTCGAAACCAAGATGGTCATGGCTTGGATGCCCACAAAAGCCATGAAGTTTCGAGAAATAGACGACAATCTATTCCTATTTATATTCGGAAACATGGAAGACAAGCAGAGAGTGCTTGACAATGGCCCTTGGCATTTTGAAAATGCCTTGTTGGTTCTGGACTACACAGATGGGACTGTGAGACCATCTGAAATGAAGCTGAATCATGCAGATTTATGGGTCCAAGTGCACAACTTGCCATTGTTGGGTATGAATGTAGCCCTTGGGAGAATGATAGCCAACTGTTTGGGAAAATGCCTAGAGGTTGAGGAAGACGGTGATGGAGAATGTTGGAGAAATTGTATGAGGTTGAGAGTGAGGTTGGATGCCACTAAGCCATTGCGGCGGGGCATGAAGATTTGTCTTGAAGGGTGTGAGCCATTTTGGGTGGATTTCAAGTATGAAGGGGTACCGAAGTTTTGTTACCATTGTGGGATTTGGGGGCACAATGAGAGTGAATGTGAAGTTGTGATTGGGAAGGAAAGGGGATTTTTGTCTGTGACATTTTCTCGGATGTGGGAGTATGTATCTTCTTGGGTTGGATTGTGGTCGAAGGAAGAGGAAGCGATTGATTTTGGGAGTGGAAATGAAGATACGAAGAAGCCAGAGGTGATTGAGGAATCGAAGGAATGTGGTCCGAAGCTTAGCCATACAGAAGGACCGGAGAGCAATGGTGATCTGGGAGACTTAACTAAGGTTGGATTTCGATTTGGTTCCATAGGGCAACATGATGTTAGTGCAGATGATTGGACAAAGGGTGGCTTTCAATCTGGTTCTGCAGTGCCAAAGAGCTGCAAGCAGACAAGAGTCATCAGAAAGGTGATCAGGCGAAATAGAAGTAACCAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

352

Amino Acids

40.1

Weight (kDa)

5.62

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 37 - 110 1.4e-10 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 177 - 224 8.5e-14 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 616
AccB1I GGYRCC 1 cut(s) 616
AciI CCGC 2 cut(s) 550, 553
AclWI GGATC 1 cut(s) 64
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 2 cut(s) 124, 413
AgsI TTSAA 4 cut(s) 308, 575, 604, 974
AjiI CACGTC 1 cut(s) 76
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
AluBI AGCT 4 cut(s) 53, 367, 850, 999
AluI AGCT 4 cut(s) 53, 367, 850, 999
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 2 cut(s) 343, 885
Alw44I GTGCAC 1 cut(s) 395
AlwI GGATC 1 cut(s) 64
AoxI GGCC 1 cut(s) 292
ApaLI GTGCAC 1 cut(s) 395
ApeKI GCWGC 1 cut(s) 999
Asp718I GGTACC 1 cut(s) 616
AspS9I GGNCC 5 cut(s) 136, 293, 388, 842, 866
AsuHPI GGTGA 5 cut(s) 25, 497, 829, 893, 1039
AsuII TTCGAA 1 cut(s) 171
AvaII GGWCC 4 cut(s) 136, 388, 842, 866
BaeGI GKGCMC 2 cut(s) 399, 654
BaeI ACNNNNGTAYC 2 cut(s) 608, 641
BanI GGYRCC 1 cut(s) 616
BarI GAAGNNNNNNTAC 2 cut(s) 614, 646
BbsI GAAGAC 2 cut(s) 273, 486
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 1 cut(s) 986
BccI CCATC 4 cut(s) 175, 332, 361, 482
BclI TGATCA 1 cut(s) 1029
BcoDI GTCTC 2 cut(s) 343, 885
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 2 cut(s) 157, 984
BglI GCCNNNNNGGC 1 cut(s) 550
BisI GCNGC 2 cut(s) 551, 1000
BlpI GCTNAGC 2 cut(s) 54, 851
BlsI GCNGC 2 cut(s) 552, 1001
Bme18I GGWCC 4 cut(s) 136, 388, 842, 866
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 5 cut(s) 136, 293, 388, 842, 866
BmiI GGNNCC 3 cut(s) 389, 618, 922
BmsI GCATC 2 cut(s) 186, 523
BoxI GACNNNNGTC 1 cut(s) 1013
BpiI GAAGAC 2 cut(s) 273, 486
Bpu1102I GCTNAGC 2 cut(s) 54, 851
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 296, 430
BsaWI WCCGGW 1 cut(s) 868
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 413
Bse3DI GCAATG 2 cut(s) 545, 883
BseDI CCNNGG 2 cut(s) 296, 430
BseGI GGATG 3 cut(s) 201, 538, 726
BseLI CCNNNNNNNGG 2 cut(s) 124, 413
BseMI GCAATG 2 cut(s) 545, 883
BseMII CTCAG 1 cut(s) 45
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 2 cut(s) 399, 654
BseXI GCAGC 1 cut(s) 986
BsgI GTGCAG 1 cut(s) 966
BshFI GGCC 1 cut(s) 294
BshNI GGYRCC 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 1 cut(s) 869
BslFI GGGAC 2 cut(s) 82, 355
BslI CCNNNNNNNGG 2 cut(s) 124, 413
BsmAI GTCTC 2 cut(s) 343, 885
BsmFI GGGAC 2 cut(s) 82, 355
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 1 cut(s) 294
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 399, 654
Bsp143I GATC 3 cut(s) 69, 883, 1029
Bsp1720I GCTNAGC 2 cut(s) 54, 851
BspACI CCGC 2 cut(s) 550, 553
BspANI GGCC 1 cut(s) 294
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 3 cut(s) 389, 618, 922
BspMAI CTGCAG 2 cut(s) 161, 988
BspPI GGATC 1 cut(s) 64
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 545, 883
BssECI CCNNGG 2 cut(s) 296, 430
BssMI GATC 3 cut(s) 69, 883, 1029
BssT1I CCWWGG 2 cut(s) 296, 430
Bst4CI ACNGT 3 cut(s) 346, 452, 485
Bst6I CTCTTC 1 cut(s) 759
BstAPI GCANNNNNTGC 2 cut(s) 280, 403
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 1004
BstDEI CTNAG 4 cut(s) 54, 540, 851, 900
BstF5I GGATG 3 cut(s) 201, 538, 726
BstKTI GATC 3 cut(s) 72, 886, 1032
BstMAI GTCTC 2 cut(s) 343, 885
BstMBI GATC 3 cut(s) 69, 883, 1029
BstMWI GCNNNNNNNGC 3 cut(s) 280, 403, 550
BstPAI GACNNNNGTC 1 cut(s) 1013
BstSFI CTRYAG 2 cut(s) 157, 984
BstSLI GKGCMC 2 cut(s) 399, 654
BstV1I GCAGC 1 cut(s) 986
BstV2I GAAGAC 2 cut(s) 273, 486
BstXI CCANNNNNNTGG 1 cut(s) 454
BsuRI GGCC 1 cut(s) 294
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 3 cut(s) 201, 538, 726
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 2 cut(s) 45, 993
Cac8I GCNNGC 1 cut(s) 1004
Cfr13I GGNCC 5 cut(s) 136, 293, 388, 842, 866
Csp6I GTAC 1 cut(s) 617
CviAII CATG 6 cut(s) 187, 211, 262, 374, 559, 935
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 4 cut(s) 54, 540, 851, 900
DpnI GATC 3 cut(s) 71, 885, 1031
DpnII GATC 3 cut(s) 69, 883, 1029
Eam1104I CTCTTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 759
Eco130I CCWWGG 2 cut(s) 296, 430
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 136, 388, 842, 866
Eco57I CTGAAG 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 296, 430
ErhI CCWWGG 2 cut(s) 296, 430
FaeI CATG 6 cut(s) 190, 214, 265, 377, 562, 938
FalI AAGNNNNNCTT 2 cut(s) 953, 985
FaqI GGGAC 2 cut(s) 82, 355
FatI CATG 6 cut(s) 186, 210, 261, 373, 558, 934
FauI CCCGC 1 cut(s) 546
FbaI TGATCA 1 cut(s) 1029
Fnu4HI GCNGC 2 cut(s) 551, 1000
FokI GGATG 3 cut(s) 208, 545, 733
Fsp4HI GCNGC 2 cut(s) 551, 1000
FspBI CTAG 1 cut(s) 467
GluI GCNGC 2 cut(s) 551, 1000
HaeIII GGCC 1 cut(s) 294
HapII CCGG 1 cut(s) 869
Hin1II CATG 6 cut(s) 190, 214, 265, 377, 562, 938
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 5 cut(s) 95, 162, 370, 827, 1014
HpaII CCGG 1 cut(s) 869
HphI GGTGA 5 cut(s) 25, 497, 829, 893, 1039
Hpy166II GTNNAC 1 cut(s) 397
Hpy188I TCNGA 5 cut(s) 257, 358, 720, 846, 1022
Hpy188III TCNNGA 4 cut(s) 103, 221, 326, 572
Hpy8I GTNNAC 1 cut(s) 397
HpyAV CCTTC 7 cut(s) 163, 569, 605, 679, 754, 826, 857
HpyCH4III ACNGT 3 cut(s) 346, 452, 485
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 7 cut(s) 29, 159, 377, 397, 947, 986, 1002
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 403, 550
HpyF3I CTNAG 4 cut(s) 54, 540, 851, 900
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 6 cut(s) 190, 214, 265, 377, 562, 938
KpnI GGTACC 1 cut(s) 620
Ksp22I TGATCA 1 cut(s) 1029
Kzo9I GATC 3 cut(s) 69, 883, 1029
LpnPI CCDG 9 cut(s) 15, 44, 110, 311, 825, 872, 882, 963, 1018
Lsp1109I GCAGC 1 cut(s) 986
LweI GCATC 2 cut(s) 186, 523
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 629, 706, 1046
MalI GATC 3 cut(s) 71, 885, 1031
MboI GATC 3 cut(s) 69, 883, 1029
MboII GAAGA 7 cut(s) 278, 491, 574, 729, 776, 809, 817
MhlI GDGCHC 2 cut(s) 399, 654
MluCI AATT 3 cut(s) 36, 110, 505
MlyI GAGTC 2 cut(s) 104, 1023
MmeI TCCRAC 4 cut(s) 479, 510, 727, 886
MseI TTAA 2 cut(s) 896, 1057
MspI CCGG 1 cut(s) 869
Mva1269I GAATGC 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 280, 403, 550
NdeII GATC 3 cut(s) 69, 883, 1029
NlaIII CATG 6 cut(s) 190, 214, 265, 377, 562, 938
NlaIV GGNNCC 3 cut(s) 389, 618, 922
NmuCI GTSAC 1 cut(s) 706
NspV TTCGAA 1 cut(s) 171
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 3 cut(s) 162, 370, 827
PkrI GCNGC 2 cut(s) 552, 1001
PleI GAGTC 2 cut(s) 103, 1022
PpsI GAGTC 2 cut(s) 103, 1022
PpuMI RGGWCCY 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 389, 618, 922
PspPI GGNCC 5 cut(s) 136, 293, 388, 842, 866
PspPPI RGGWCCY 1 cut(s) 136
PstI CTGCAG 2 cut(s) 161, 988
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
SaqAI TTAA 2 cut(s) 896, 1057
SatI GCNGC 2 cut(s) 551, 1000
Sau3AI GATC 3 cut(s) 69, 883, 1029
Sau96I GGNCC 5 cut(s) 136, 293, 388, 842, 866
SchI GAGTC 2 cut(s) 104, 1023
SduI GDGCHC 2 cut(s) 399, 654
SfaNI GCATC 2 cut(s) 186, 523
SfcI CTRYAG 2 cut(s) 157, 984
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 4 cut(s) 136, 388, 842, 866
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
Sse9I AATT 3 cut(s) 36, 110, 505
SsiI CCGC 2 cut(s) 550, 553
SspMI CTAG 1 cut(s) 467
StyI CCWWGG 2 cut(s) 296, 430
TaaI ACNGT 3 cut(s) 346, 452, 485
TaiI ACGT 1 cut(s) 78
TaqI TCGA 5 cut(s) 171, 220, 758, 830, 913
TasI AATT 3 cut(s) 36, 110, 505
TauI GCSGC 1 cut(s) 553
TfiI GAWTC 3 cut(s) 162, 370, 827
Tru1I TTAA 2 cut(s) 896, 1057
Tru9I TTAA 2 cut(s) 896, 1057
TscAI CASTG 2 cut(s) 52, 993
TseFI GTSAC 1 cut(s) 706
TseI GCWGC 1 cut(s) 999
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 6 cut(s) 227, 377, 434, 575, 624, 810
TspRI CASTG 2 cut(s) 52, 993
VneI GTGCAC 1 cut(s) 395
VpaK11BI GGWCC 4 cut(s) 136, 388, 842, 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.