Rh5AG195300

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004190
Physical Location & Seq
Forward (+)
22688091 .. 22690483
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG195300.1

Sequence Viewer

Length: 1101 bp
ATGGATTATGGAGCTGTTGAAGGTGGAATAACTGATAACAGCCAGGCATTTTTGAAAGCATGGAATGATGCCTGTAAATGGACTGGAAGGGCAAGGGTTTTGGTTCCCCGAGGAATATTCAAGTTATTCCCAGTTGTATTCTCAGGTCCATGCAATGGTCCGATTGCCTTTTTGATCATGGGGACTTTAAGAGCCTCTACTAATCCATCAACATTTAATTCTGAAAATTGGATAAACTTCCGATACTTGGAGCAGTTAACCGTAGCCGGAGGCGGTACCTTGGACGGCCAAGGATCCTTTGTTTGGCCTCTTAACAACTGCAACAATCGTCCATGCAAGACACTCCCCATCTCTTTGAGTTTTGACTTCGTCACAAATGCAAGCGTCGCTCACTTGAGATCATACAACAGCAAAAATGCCCATATCAAGGTTTATGGATGCAACAAAATGGACTTCACCAAACTTAGATTGTCAGCTCCAGGTGATAGCCCAAACACCGATGGCATAAAAATCGGAAACTCCTACCGCATCAGAATCTCACGCTCGGTTATTGGCACCGGCGATGACTGCATTGCGATATTGTCAGGAAGTAGGGAAATCCACATTTCTAAAATTTTTTGCGGTCCTGGGCATGGTATCAGCATTGGAAGTCTCGGAGGTTATGATAATGAATACGATGTGGAAGGAATATATGTGAAAGATAGCGGTTTAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTTATTCTCACCCCTTGAAGGTTTCAAAGATTAGATACGAAAACATTGTGATGACTGATGTCACGAATCCAATTATTATCGATCAAAATTATTGCCCCAGCCCTCCCTGCAATCAACAGGTTACTTCGAATGTGCAAATTAGTGATGTGACATATAATAACATATGGGGAAGTTCTTCTTCCAAAGAAGCAGTAATTCTGAAATGCAGCAAAACCAGCCCATGCAAGAACATGGTGATGAACAATATCAAGTTATCCCTCAATGGTCCTGGAGGTATTGCGAGTTCTTCCTGCGAAAATGCCATTGGCGTCTCTTACGGCACACAAAAGCCTCCTTCTTGCATAAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

366

Amino Acids

39.64

Weight (kDa)

9.02

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 25 - 350 3.4e-99 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 275, 554
AccB7I CCANNNNNTGG 1 cut(s) 155
AciI CCGC 4 cut(s) 273, 526, 621, 705
AclWI GGATC 2 cut(s) 288, 301
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 1056
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 7 cut(s) 78, 155, 247, 303, 427, 632, 766
AgsI TTSAA 5 cut(s) 20, 55, 121, 766, 774
AjnI CCWGG 4 cut(s) 42, 478, 625, 1015
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AluBI AGCT 2 cut(s) 14, 476
AluI AGCT 2 cut(s) 14, 476
Alw26I GTCTC 2 cut(s) 656, 1063
AlwI GGATC 2 cut(s) 288, 301
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 2 cut(s) 286, 305
ApeKI GCWGC 1 cut(s) 954
ApoI RAATTY 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 922
Asp718I GGTACC 1 cut(s) 275
AspS9I GGNCC 4 cut(s) 146, 158, 623, 1013
AsuHPI GGTGA 4 cut(s) 448, 494, 749, 994
AsuII TTCGAA 1 cut(s) 875
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 4 cut(s) 146, 158, 623, 1013
BamHI GGATCC 1 cut(s) 293
BanI GGYRCC 2 cut(s) 275, 554
BbvI GCAGC 1 cut(s) 966
BccI CCATC 3 cut(s) 214, 356, 494
BceAI ACGGC 2 cut(s) 301, 1081
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 4 cut(s) 44, 480, 627, 1017
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 656, 1063
BfaI CTAG 1 cut(s) 1099
BisI GCNGC 1 cut(s) 955
BlsI GCNGC 1 cut(s) 956
Bme1390I CCNGG 4 cut(s) 44, 480, 627, 1017
Bme18I GGWCC 4 cut(s) 146, 158, 623, 1013
BmeT110I CYCGRG 1 cut(s) 108
BmgT120I GGNCC 4 cut(s) 146, 158, 623, 1013
BmiI GGNNCC 4 cut(s) 105, 277, 295, 556
BmrFI CCNGG 4 cut(s) 44, 480, 627, 1017
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 3 cut(s) 58, 428, 537
BmuI ACTGGG 1 cut(s) 125
BoxI GACNNNNGTC 1 cut(s) 806
BpmI CTGGAG 2 cut(s) 462, 1038
Bpu14I TTCGAA 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 828
BsaHI GRCGYC 1 cut(s) 1056
BsaJI CCNNGG 4 cut(s) 109, 279, 289, 626
Bsc4I CCNNNNNNNGG 7 cut(s) 78, 155, 247, 303, 427, 632, 766
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 2 cut(s) 88, 131
Bse3DI GCAATG 2 cut(s) 160, 570
BseBI CCWGG 4 cut(s) 44, 480, 627, 1017
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 4 cut(s) 109, 279, 289, 626
BseGI GGATG 1 cut(s) 443
BseLI CCNNNNNNNGG 7 cut(s) 78, 155, 247, 303, 427, 632, 766
BseMI GCAATG 2 cut(s) 160, 570
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 88, 131
BseXI GCAGC 1 cut(s) 966
BseYI CCCAGC 1 cut(s) 845
BshFI GGCC 2 cut(s) 288, 307
BshNI GGYRCC 2 cut(s) 275, 554
BshVI ATCGAT 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 2 cut(s) 267, 558
BslFI GGGAC 2 cut(s) 196, 730
BslI CCNNNNNNNGG 7 cut(s) 78, 155, 247, 303, 427, 632, 766
BsmAI GTCTC 2 cut(s) 656, 1063
BsmBI CGTCTC 1 cut(s) 1063
BsmFI GGGAC 2 cut(s) 196, 730
BsnI GGCC 2 cut(s) 288, 307
BsoBI CYCGRG 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 875
Bsp143I GATC 4 cut(s) 174, 293, 398, 829
BspACI CCGC 4 cut(s) 273, 526, 621, 705
BspANI GGCC 2 cut(s) 288, 307
BspCNI CTCAG 1 cut(s) 155
BspDI ATCGAT 1 cut(s) 828
BspLI GGNNCC 4 cut(s) 105, 277, 295, 556
BspPI GGATC 2 cut(s) 288, 301
BspT104I TTCGAA 1 cut(s) 875
BspT107I GGYRCC 2 cut(s) 275, 554
BsrDI GCAATG 2 cut(s) 160, 570
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 2 cut(s) 88, 131
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 4 cut(s) 109, 279, 289, 626
BssMI GATC 4 cut(s) 174, 293, 398, 829
BssNI GRCGYC 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 279, 289
Bst2UI CCWGG 4 cut(s) 44, 480, 627, 1017
Bst4CI ACNGT 1 cut(s) 262
BstACI GRCGYC 1 cut(s) 1056
BstBI TTCGAA 1 cut(s) 875
BstC8I GCNNGC 1 cut(s) 382
BstDEI CTNAG 3 cut(s) 142, 464, 731
BstF5I GGATG 1 cut(s) 443
BstKTI GATC 4 cut(s) 177, 296, 401, 832
BstMAI GTCTC 2 cut(s) 656, 1063
BstMBI GATC 4 cut(s) 174, 293, 398, 829
BstMWI GCNNNNNNNGC 4 cut(s) 386, 567, 855, 963
BstNI CCWGG 4 cut(s) 44, 480, 627, 1017
BstPAI GACNNNNGTC 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 42, 478, 625, 1015
BstV1I GCAGC 1 cut(s) 966
BstX2I RGATCY 1 cut(s) 293
BstYI RGATCY 1 cut(s) 293
Bsu15I ATCGAT 1 cut(s) 828
BsuRI GGCC 2 cut(s) 288, 307
BsuTUI ATCGAT 1 cut(s) 828
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 382
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 4 cut(s) 146, 158, 623, 1013
ClaI ATCGAT 1 cut(s) 828
CseI GACGC 2 cut(s) 373, 1045
Csp6I GTAC 1 cut(s) 276
CviAII CATG 8 cut(s) 60, 150, 178, 333, 632, 744, 969, 979
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 3 cut(s) 142, 464, 731
DpnI GATC 4 cut(s) 176, 295, 400, 831
DpnII GATC 4 cut(s) 174, 293, 398, 829
DraI TTTAAA 1 cut(s) 711
EaeI YGGCCR 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 279, 289
Eco47I GGWCC 4 cut(s) 146, 158, 623, 1013
Eco88I CYCGRG 1 cut(s) 108
EcoRII CCWGG 4 cut(s) 42, 478, 625, 1015
EcoT14I CCWWGG 2 cut(s) 279, 289
ErhI CCWWGG 2 cut(s) 279, 289
Esp3I CGTCTC 1 cut(s) 1063
FaeI CATG 8 cut(s) 63, 153, 181, 336, 635, 747, 972, 982
FalI AAGNNNNNCTT 2 cut(s) 910, 942
FaqI GGGAC 2 cut(s) 196, 730
FatI CATG 8 cut(s) 59, 149, 177, 332, 631, 743, 968, 978
FauNDI CATATG 1 cut(s) 911
FbaI TGATCA 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 955
FokI GGATG 1 cut(s) 450
Fsp4HI GCNGC 1 cut(s) 955
FspBI CTAG 1 cut(s) 1099
GluI GCNGC 1 cut(s) 955
GsaI CCCAGC 1 cut(s) 849
GsuI CTGGAG 2 cut(s) 462, 1038
HaeIII GGCC 2 cut(s) 288, 307
HapII CCGG 2 cut(s) 267, 558
HgaI GACGC 2 cut(s) 373, 1045
Hin1I GRCGYC 1 cut(s) 1056
Hin1II CATG 8 cut(s) 63, 153, 181, 336, 635, 747, 972, 982
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 3 cut(s) 534, 735, 814
HpaI GTTAAC 1 cut(s) 258
HpaII CCGG 2 cut(s) 267, 558
HphI GGTGA 4 cut(s) 448, 494, 749, 994
Hpy166II GTNNAC 1 cut(s) 258
Hpy188I TCNGA 7 cut(s) 162, 223, 242, 515, 533, 656, 948
Hpy188III TCNNGA 2 cut(s) 585, 811
Hpy8I GTNNAC 1 cut(s) 258
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 5 cut(s) 14, 81, 677, 760, 1092
HpyCH4III ACNGT 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 4 cut(s) 386, 567, 855, 963
HpyF3I CTNAG 3 cut(s) 142, 464, 731
Hsp92I GRCGYC 1 cut(s) 1056
Hsp92II CATG 8 cut(s) 63, 153, 181, 336, 635, 747, 972, 982
KpnI GGTACC 1 cut(s) 279
Ksp22I TGATCA 1 cut(s) 174
KspAI GTTAAC 1 cut(s) 258
Kzo9I GATC 4 cut(s) 174, 293, 398, 829
LmnI GCTCC 3 cut(s) 11, 250, 481
Lsp1109I GCAGC 1 cut(s) 966
LweI GCATC 3 cut(s) 58, 428, 537
MaeI CTAG 1 cut(s) 1099
MaeIII GTNAC 4 cut(s) 370, 808, 868, 895
MalI GATC 4 cut(s) 176, 295, 400, 831
MboI GATC 4 cut(s) 174, 293, 398, 829
MboII GAAGA 3 cut(s) 915, 918, 1026
MflI RGATCY 1 cut(s) 293
MluCI AATT 7 cut(s) 217, 226, 612, 819, 835, 885, 942
MnlI CCTC 9 cut(s) 104, 205, 263, 318, 650, 861, 1013, 1016, 1089
MroXI GAANNNNTTC 1 cut(s) 922
MseI TTAA 5 cut(s) 188, 216, 257, 312, 710
MslI CAYNNNNRTG 2 cut(s) 797, 983
MspI CCGG 2 cut(s) 267, 558
MspR9I CCNGG 4 cut(s) 44, 480, 627, 1017
MvaI CCWGG 4 cut(s) 44, 480, 627, 1017
MwoI GCNNNNNNNGC 4 cut(s) 386, 567, 855, 963
NdeI CATATG 1 cut(s) 911
NdeII GATC 4 cut(s) 174, 293, 398, 829
NlaIII CATG 8 cut(s) 63, 153, 181, 336, 635, 747, 972, 982
NlaIV GGNNCC 4 cut(s) 105, 277, 295, 556
NmuCI GTSAC 3 cut(s) 370, 808, 895
NspV TTCGAA 1 cut(s) 875
PdmI GAANNNNTTC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 534, 735, 814
PflFI GACNNNGTC 1 cut(s) 368
PflMI CCANNNNNTGG 1 cut(s) 155
PfoI TCCNGGA 1 cut(s) 1015
PkrI GCNGC 1 cut(s) 956
PshAI GACNNNNGTC 1 cut(s) 806
Psp6I CCWGG 4 cut(s) 42, 478, 625, 1015
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 4 cut(s) 42, 478, 625, 1015
PspN4I GGNNCC 4 cut(s) 105, 277, 295, 556
PspPI GGNCC 4 cut(s) 146, 158, 623, 1013
PsrI GAACNNNNNNTAC 2 cut(s) 1015, 1047
PsuI RGATCY 1 cut(s) 293
PsyI GACNNNGTC 1 cut(s) 368
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 2 cut(s) 797, 983
SaqAI TTAA 5 cut(s) 188, 216, 257, 312, 710
SatI GCNGC 1 cut(s) 955
Sau3AI GATC 4 cut(s) 174, 293, 398, 829
Sau96I GGNCC 4 cut(s) 146, 158, 623, 1013
ScrFI CCNGG 4 cut(s) 44, 480, 627, 1017
SfaNI GCATC 3 cut(s) 58, 428, 537
SfuI TTCGAA 1 cut(s) 875
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 4 cut(s) 146, 158, 623, 1013
SmiMI CAYNNNNRTG 2 cut(s) 797, 983
SmlI CTYRAG 1 cut(s) 394
SmoI CTYRAG 1 cut(s) 394
Sse9I AATT 7 cut(s) 217, 226, 612, 819, 835, 885, 942
SsiI CCGC 4 cut(s) 273, 526, 621, 705
SspI AATATT 1 cut(s) 117
SspMI CTAG 1 cut(s) 1099
StyD4I CCNGG 4 cut(s) 42, 478, 625, 1015
StyI CCWWGG 2 cut(s) 279, 289
TaaI ACNGT 1 cut(s) 262
TaqI TCGA 2 cut(s) 828, 875
TasI AATT 7 cut(s) 217, 226, 612, 819, 835, 885, 942
TfiI GAWTC 3 cut(s) 534, 735, 814
Tru1I TTAA 5 cut(s) 188, 216, 257, 312, 710
Tru9I TTAA 5 cut(s) 188, 216, 257, 312, 710
TseFI GTSAC 3 cut(s) 370, 808, 895
TseI GCWGC 1 cut(s) 954
Tsp45I GTSAC 3 cut(s) 370, 808, 895
TspDTI ATGAA 2 cut(s) 684, 1001
Tth111I GACNNNGTC 1 cut(s) 368
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 4 cut(s) 146, 158, 623, 1013
XapI RAATTY 1 cut(s) 612
XmnI GAANNNNTTC 1 cut(s) 922
XspI CTAG 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.