MD10G1194900.v1.1

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004190
Physical Location & Seq
Reverse (-)
29293170 .. 29294970
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1194900.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGGGTTCGATCATATATTCTGTAGGTAGGGTCTTCTTGTTATTCTTCTTGTTGGTATGGGAAACAGAAGGTCGTGATCAAGCCAAGTATTTCGACGTGAGGAAATATGGAGCTGTTGATGATGGAAAAACTGATAACAGCCAGGCATTTCTGGATGCATGGAAAGAAGCATGTCAATGGAAAGGAACGGCAAGGGTTTTGGTTCCCAGAGGAACATTCAAGTTGTATCCAGTGATTTTCTCAGGTCCATGCAATGGTCCGATTGCCTTTCTGATTAAGGGTACTTTGAGGGCTACGACTAATCCATCAACATTTAGTGCTCACAGTTGGATAAACTTCCGGTACATCGATCAGTTGACGGTGACCGGAGGCGGTACCTTGGACGGCCAAGGAGCCTCGGCTTGGCATCTTAACAACTGCAAAACCAATCCCCAATGCCAGGCTCTTCCTGTTTCAATGAGTTTTGACTTCATCACAAACGCAAGAATCGAATACATAAGATCATACGACAGCAAAAATGCACATTTCAAAATTTTCGGATGCTACAATATGGACTTTAGGAAAATCAAAATCTCAGCTCCGGCTGACAGTCCGAACACCGATGGCATTAAGATTGGAAGCTCATACCGAATCAGAATCGCACGCTCTACGATCGCCACAGGCGATGACTGTGTCGCGATGTTGCACGGAAGTAGGAAAATCCACATTTCTAAAGTTGTGTGCGGTCCCGGGCATGGAATCAGCATCGGAAGCCTAGGAGGGAATGACAAAGAAGACGATGTGGTCGGAGTGATTGTGAAAAACTGCACCTTTATGGGGACGGATAATGGTGTGAGGATCAAAACTTGGGCTTCTCCTTATAGCAGCAAGGCTTCAAATTTTACATTCCAAGACATTTTCTTGGATGACGTTCTGAATCCTATCACCATCGATCAAGAATATTGCCCCAACCCTCCTTGCAATCAACAGACTTCTTCGAATGTGCAAATCAGGGACGTTACGTACAAAAACATATGGGGAAGTTCATCGTCGGAAGTAGCCGTCAGTCTCCGATGCAGCAAAACCTGGCCATGCAAGAATGTACTGCTGGACAGCATCAAGATGTCCTATTCTAATCCCAGAGGACGTTTGCGTTCCTTCTGCAATTATGCCAAAGGCTCCTCTTATGGCTCACAAAGCCCTCCATCTTGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

399

Amino Acids

43.93

Weight (kDa)

9.03

Isoelectric Point (pI)

30.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 58 - 373 3.6e-94 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 782
Acc65I GGTACC 1 cut(s) 374
AccB1I GGYRCC 1 cut(s) 374
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 1 cut(s) 677
AciI CCGC 2 cut(s) 372, 723
AclWI GGATC 1 cut(s) 845
AcoI YGGCCR 2 cut(s) 385, 1067
AcsI RAATTY 2 cut(s) 531, 877
AfaI GTAC 5 cut(s) 283, 344, 376, 1004, 1083
AfiI CCNNNNNNNGG 5 cut(s) 254, 402, 439, 734, 816
AgsI TTSAA 4 cut(s) 220, 456, 529, 876
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 3 cut(s) 141, 438, 1064
AluBI AGCT 3 cut(s) 113, 578, 621
AluI AGCT 3 cut(s) 113, 578, 621
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 1 cut(s) 845
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 2 cut(s) 385, 1067
ApeKI GCWGC 2 cut(s) 864, 1056
ApoI RAATTY 2 cut(s) 531, 877
Asp718I GGTACC 1 cut(s) 374
AspA2I CCTAGG 1 cut(s) 754
AspS9I GGNCC 3 cut(s) 245, 257, 725
AsuC2I CCSGG 2 cut(s) 729, 730
AsuHPI GGTGA 2 cut(s) 373, 916
AsuII TTCGAA 1 cut(s) 977
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 3 cut(s) 245, 257, 725
AvrII CCTAGG 1 cut(s) 754
BalI TGGCCA 1 cut(s) 1069
BanI GGYRCC 1 cut(s) 374
BbsI GAAGAC 2 cut(s) 25, 780
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 2 cut(s) 876, 1068
BccI CCATC 5 cut(s) 116, 313, 596, 935, 1192
BceAI ACGGC 3 cut(s) 204, 400, 1025
BciT130I CCWGG 3 cut(s) 143, 440, 1066
BciVI GTATCC 1 cut(s) 237
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 2 cut(s) 729, 730
BcoDI GTCTC 1 cut(s) 1052
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 1 cut(s) 21
BfuI GTATCC 1 cut(s) 237
BisI GCNGC 2 cut(s) 865, 1057
BlnI CCTAGG 1 cut(s) 754
BlsI GCNGC 2 cut(s) 866, 1058
Bme1390I CCNGG 5 cut(s) 143, 440, 729, 730, 1066
Bme18I GGWCC 3 cut(s) 245, 257, 725
BmeT110I CYCGRG 1 cut(s) 728
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 3 cut(s) 245, 257, 725
BmiI GGNNCC 5 cut(s) 204, 376, 394, 727, 1159
BmrFI CCNGG 5 cut(s) 143, 440, 729, 730, 1066
BmsI GCATC 6 cut(s) 145, 415, 530, 753, 1043, 1104
BpiI GAAGAC 2 cut(s) 25, 780
Bpu14I TTCGAA 1 cut(s) 977
BpuMI CCSGG 2 cut(s) 729, 730
Bsa29I ATCGAT 2 cut(s) 348, 930
BsaAI YACGTR 1 cut(s) 1002
BsaJI CCNNGG 5 cut(s) 378, 388, 396, 728, 754
BsaWI WCCGGW 2 cut(s) 339, 365
BsaXI ACNNNNNCTCC 2 cut(s) 780, 810
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 402, 439, 734, 816
Bse1I ACTGG 1 cut(s) 230
Bse3DI GCAATG 1 cut(s) 259
BseBI CCWGG 3 cut(s) 143, 440, 1066
BseCI ATCGAT 2 cut(s) 348, 930
BseDI CCNNGG 5 cut(s) 378, 388, 396, 728, 754
BseGI GGATG 3 cut(s) 160, 545, 910
BseLI CCNNNNNNNGG 5 cut(s) 254, 402, 439, 734, 816
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 255, 588
BseNI ACTGG 1 cut(s) 230
BseRI GAGGAG 1 cut(s) 1150
BseXI GCAGC 2 cut(s) 876, 1068
BsgI GTGCAG 1 cut(s) 790
Bsh1236I CGCG 1 cut(s) 677
Bsh1285I CGRYCG 1 cut(s) 654
BshFI GGCC 2 cut(s) 387, 1069
BshNI GGYRCC 1 cut(s) 374
BshVI ATCGAT 2 cut(s) 348, 930
BsiEI CGRYCG 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 322
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 4 cut(s) 340, 366, 581, 729
BslFI GGGAC 3 cut(s) 711, 832, 1007
BslI CCNNNNNNNGG 5 cut(s) 254, 402, 439, 734, 816
BsmAI GTCTC 1 cut(s) 1052
BsmFI GGGAC 3 cut(s) 711, 832, 1007
BsnI GGCC 2 cut(s) 387, 1069
BsoBI CYCGRG 1 cut(s) 728
Bsp119I TTCGAA 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
Bsp68I TCGCGA 1 cut(s) 677
BspACI CCGC 2 cut(s) 372, 723
BspANI GGCC 2 cut(s) 387, 1069
BspCNI CTCAG 2 cut(s) 254, 587
BspDI ATCGAT 2 cut(s) 348, 930
BspFNI CGCG 1 cut(s) 677
BspLI GGNNCC 5 cut(s) 204, 376, 394, 727, 1159
BspPI GGATC 1 cut(s) 845
BspQI GCTCTTC 1 cut(s) 450
BspT104I TTCGAA 1 cut(s) 977
BspT107I GGYRCC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 5 cut(s) 378, 388, 396, 728, 754
BssMI GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
BssT1I CCWWGG 3 cut(s) 378, 388, 754
Bst2UI CCWGG 3 cut(s) 143, 440, 1066
Bst4CI ACNGT 4 cut(s) 326, 361, 590, 671
Bst6I CTCTTC 1 cut(s) 450
BstBAI YACGTR 1 cut(s) 1002
BstBI TTCGAA 1 cut(s) 977
BstC8I GCNNGC 1 cut(s) 643
BstDEI CTNAG 2 cut(s) 241, 574
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 3 cut(s) 160, 545, 910
BstFNI CGCG 1 cut(s) 677
BstKTI GATC 7 cut(s) 12, 79, 352, 503, 654, 840, 934
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
BstMCI CGRYCG 1 cut(s) 654
BstMWI GCNNNNNNNGC 2 cut(s) 750, 1176
BstNI CCWGG 3 cut(s) 143, 440, 1066
BstNSI RCATGY 1 cut(s) 174
BstPI GGTNACC 1 cut(s) 361
BstSCI CCNGG 5 cut(s) 141, 438, 727, 728, 1064
BstSFI CTRYAG 1 cut(s) 21
BstSNI TACGTA 1 cut(s) 1002
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 2 cut(s) 876, 1068
BstV2I GAAGAC 2 cut(s) 25, 780
Bsu15I ATCGAT 2 cut(s) 348, 930
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 2 cut(s) 387, 1069
BsuTUI ATCGAT 2 cut(s) 348, 930
BtgZI GCGATG 2 cut(s) 678, 692
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 160, 545, 910
BtsIMutI CAGTG 1 cut(s) 237
BtuMI TCGCGA 1 cut(s) 677
Cac8I GCNNGC 1 cut(s) 643
Cfr13I GGNCC 3 cut(s) 245, 257, 725
Cfr9I CCCGGG 1 cut(s) 728
ClaI ATCGAT 2 cut(s) 348, 930
Csp6I GTAC 5 cut(s) 282, 343, 375, 1003, 1082
CviAII CATG 5 cut(s) 159, 171, 249, 734, 1071
CviQI GTAC 5 cut(s) 282, 343, 375, 1003, 1082
DdeI CTNAG 2 cut(s) 241, 574
DpnI GATC 7 cut(s) 11, 78, 351, 502, 653, 839, 933
DpnII GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
EaeI YGGCCR 2 cut(s) 385, 1067
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco105I TACGTA 1 cut(s) 1002
Eco130I CCWWGG 3 cut(s) 378, 388, 754
Eco47I GGWCC 3 cut(s) 245, 257, 725
Eco88I CYCGRG 1 cut(s) 728
Eco91I GGTNACC 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 361
EcoRII CCWGG 3 cut(s) 141, 438, 1064
EcoT14I CCWWGG 3 cut(s) 378, 388, 754
EcoT22I ATGCAT 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 378, 388, 754
FaeI CATG 5 cut(s) 162, 174, 252, 737, 1074
FaqI GGGAC 3 cut(s) 711, 832, 1007
FatI CATG 5 cut(s) 158, 170, 248, 733, 1070
FauNDI CATATG 1 cut(s) 1013
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 865, 1057
FokI GGATG 3 cut(s) 167, 552, 917
Fsp4HI GCNGC 2 cut(s) 865, 1057
FspBI CTAG 1 cut(s) 755
GluI GCNGC 2 cut(s) 865, 1057
HaeIII GGCC 2 cut(s) 387, 1069
HapII CCGG 4 cut(s) 340, 366, 581, 729
Hin1II CATG 5 cut(s) 162, 174, 252, 737, 1074
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 5 cut(s) 486, 630, 636, 738, 916
HpaII CCGG 4 cut(s) 340, 366, 581, 729
HphI GGTGA 2 cut(s) 373, 916
Hpy166II GTNNAC 1 cut(s) 357
Hpy188III TCNNGA 5 cut(s) 74, 152, 676, 935, 1099
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 2 cut(s) 98, 1033
HpyAV CCTTC 2 cut(s) 62, 1147
HpyCH4III ACNGT 4 cut(s) 326, 361, 590, 671
HpyCH4IV ACGT 5 cut(s) 96, 909, 996, 1001, 1126
HpyF10VI GCNNNNNNNGC 2 cut(s) 750, 1176
HpyF3I CTNAG 2 cut(s) 241, 574
HpySE526I ACGT 5 cut(s) 96, 909, 996, 1001, 1126
Hsp92II CATG 5 cut(s) 162, 174, 252, 737, 1074
KpnI GGTACC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 4 cut(s) 110, 392, 583, 1163
Lsp1109I GCAGC 2 cut(s) 876, 1068
LweI GCATC 6 cut(s) 145, 415, 530, 753, 1043, 1104
MaeI CTAG 1 cut(s) 755
MaeII ACGT 5 cut(s) 96, 909, 996, 1001, 1126
MaeIII GTNAC 2 cut(s) 361, 997
MalI GATC 7 cut(s) 11, 78, 351, 502, 653, 839, 933
MboI GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
MboII GAAGA 5 cut(s) 25, 37, 437, 785, 966
MhlI GDGCHC 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 1069
MluCI AATT 3 cut(s) 531, 877, 1144
MluNI TGGCCA 1 cut(s) 1069
MmeI TCCRAC 3 cut(s) 308, 766, 1011
Mox20I TGGCCA 1 cut(s) 1069
Mph1103I ATGCAT 1 cut(s) 160
MscI TGGCCA 1 cut(s) 1069
MseI TTAA 3 cut(s) 276, 411, 609
MslI CAYNNNNRTG 3 cut(s) 175, 812, 1100
Msp20I TGGCCA 1 cut(s) 1069
MspI CCGG 4 cut(s) 340, 366, 581, 729
MspR9I CCNGG 5 cut(s) 143, 440, 729, 730, 1066
MvaI CCWGG 3 cut(s) 143, 440, 1066
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 2 cut(s) 750, 1176
NciI CCSGG 2 cut(s) 729, 730
NdeI CATATG 1 cut(s) 1013
NdeII GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
NlaIII CATG 5 cut(s) 162, 174, 252, 737, 1074
NlaIV GGNNCC 5 cut(s) 204, 376, 394, 727, 1159
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 361
NruI TCGCGA 1 cut(s) 677
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 174
NspV TTCGAA 1 cut(s) 977
PciSI GCTCTTC 1 cut(s) 450
PcsI WCGNNNNNNNCGW 2 cut(s) 486, 660
PfeI GAWTC 5 cut(s) 486, 630, 636, 738, 916
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 2 cut(s) 866, 1058
Ple19I CGATCG 1 cut(s) 654
Ppu21I YACGTR 1 cut(s) 1002
Psp6I CCWGG 3 cut(s) 141, 438, 1064
PspEI GGTNACC 1 cut(s) 361
PspGI CCWGG 3 cut(s) 141, 438, 1064
PspN4I GGNNCC 5 cut(s) 204, 376, 394, 727, 1159
PspPI GGNCC 3 cut(s) 245, 257, 725
PsyI GACNNNGTC 1 cut(s) 671
PvuI CGATCG 1 cut(s) 654
RruI TCGCGA 1 cut(s) 677
RsaI GTAC 5 cut(s) 283, 344, 376, 1004, 1083
RsaNI GTAC 5 cut(s) 282, 343, 375, 1003, 1082
RseI CAYNNNNRTG 3 cut(s) 175, 812, 1100
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 3 cut(s) 276, 411, 609
SatI GCNGC 2 cut(s) 865, 1057
Sau3AI GATC 7 cut(s) 9, 76, 349, 500, 651, 837, 931
Sau96I GGNCC 3 cut(s) 245, 257, 725
ScrFI CCNGG 5 cut(s) 143, 440, 729, 730, 1066
SduI GDGCHC 1 cut(s) 322
SfaNI GCATC 6 cut(s) 145, 415, 530, 753, 1043, 1104
SfcI CTRYAG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 977
SinI GGWCC 3 cut(s) 245, 257, 725
SmaI CCCGGG 1 cut(s) 730
SmiMI CAYNNNNRTG 3 cut(s) 175, 812, 1100
SnaBI TACGTA 1 cut(s) 1002
Sse9I AATT 3 cut(s) 531, 877, 1144
SsiI CCGC 2 cut(s) 372, 723
SspI AATATT 1 cut(s) 941
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 5 cut(s) 141, 438, 727, 728, 1064
StyI CCWWGG 3 cut(s) 378, 388, 754
TaaI ACNGT 4 cut(s) 326, 361, 590, 671
TaiI ACGT 5 cut(s) 99, 912, 999, 1004, 1129
TaqI TCGA 6 cut(s) 8, 93, 348, 489, 930, 977
TasI AATT 3 cut(s) 531, 877, 1144
TatI WGTACW 1 cut(s) 1081
TfiI GAWTC 5 cut(s) 486, 630, 636, 738, 916
Tru1I TTAA 3 cut(s) 276, 411, 609
Tru9I TTAA 3 cut(s) 276, 411, 609
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 2 cut(s) 864, 1056
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 2 cut(s) 460, 1014
TspGWI ACGGA 2 cut(s) 702, 836
TspMI CCCGGG 1 cut(s) 728
TspRI CASTG 1 cut(s) 237
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 3 cut(s) 245, 257, 725
XapI RAATTY 2 cut(s) 531, 877
XceI RCATGY 1 cut(s) 174
XmaI CCCGGG 1 cut(s) 728
XmaJI CCTAGG 1 cut(s) 754
XspI CTAG 1 cut(s) 755
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.