Rh5DG019900

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004190
Physical Location & Seq
Reverse (-)
1475781 .. 1477807
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG019900.1

Sequence Viewer

Length: 1239 bp
ATGATTGTTTTGATATCTTCCCTAGTCTTTCGGACTTTCTTGTTTTTGTTCTTGTTGGCATGGATAACAAAAGGCCATAAACTCTATCGTCAAACAGCACGTCTTGGTAGACCCATGGTTTTCAATGTGATGCGTTATGGAGCTATTGAAGGAGGAAGAACTGATAATAGTCAGGCATTTTTGAAAGCATGGAGTGAAGCATGTAAATGGGATGGAAGAGCAAAGGTTGTGATTCCGGCCGGAACATTCAAGTTGTTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTAACATGGCCTTTGTAATCAAGGGAACTTTGTTAGCTTCTACTAGTCCATCAGCATTGAGTTCTGAAAATTGGATAAACTTTCGGAACATTGAACAATTAACTGTAGCCGGGGGAGGTACACTGGACGGCCAAGGAGCCTCGGTTTGGTCACTTAACGATTGCAAAACTAATCCCGGATGCAAAGCTCTCCCAACTTCGATGACTTTCGACTTCGTAACAAATGCAAGTGTTCATCACTTAAGATCATTCAACAGCCAAAATGGTCATTTCAAGGTTCATGGATGCCAGAAGATAGACTTCGGAAACATTAGAATATCGGCTCCTAAGGACAGTCCAAACACCGATGGCATAAAAATTGGAAACTCATACCGCATCAGAATTAGACGAACGATCATTGAGACCGGCGATGATTGTATAGGAATTGTGTCGGGAAGTCAAAAAATCCACATTTCTAATGTCTTTTGCGGTCCCGGACATGGAATTAGCATTGGAAGTCTTGGAGGGCATAGTTATACAGAATATGATGTGGAGGGAATTTTTGTGAAAAATAGCGTTTTCAAAAAGACTGATAATGGTTTGAGAATCAAAACATGGGCTCGGGATTCTCCAAAACCATTGAAGGCTTCAAATATTGTGTATGAAGATATTAACATGATTGATGTCATGAATCCCATTGTCATCGATCAACAATATTGTCCGAACAATCAATGCAATCAACAGGCTTCTTCATCAAATGTGCAAATCAGCGATGTTACATTCAGAAACATATGGGGAACCTCGTCTTCCCAAGAAGCAGTGACTCTGAAATGCAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATTAAGTTACCTCATAATGGTTCCAAAGGAGCTGTGACTTCCATCTGTAATAATGTCATCGGCAAATCTTATGGCTCACACATCCCTCCTAGTTGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

412

Amino Acids

45.05

Weight (kDa)

9.42

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 69 - 398 5.9e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 109
AciI CCGC 2 cut(s) 658, 753
AcoI YGGCCR 2 cut(s) 237, 415
AcsI RAATTY 1 cut(s) 822
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 4 cut(s) 284, 432, 764, 1154
AflII CTTAAG 1 cut(s) 526
AhlI ACTAGT 1 cut(s) 329
AjiI CACGTC 1 cut(s) 101
AluBI AGCT 4 cut(s) 143, 323, 473, 1169
AluI AGCT 4 cut(s) 143, 323, 473, 1169
Alw26I GTCTC 1 cut(s) 680
Ama87I CYCGRG 1 cut(s) 885
AoxI GGCC 4 cut(s) 73, 237, 294, 415
ApeKI GCWGC 1 cut(s) 1098
ApoI RAATTY 1 cut(s) 822
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AspS9I GGNCC 2 cut(s) 275, 755
AsuC2I CCSGG 3 cut(s) 397, 462, 759
AvaI CYCGRG 1 cut(s) 885
AvaII GGWCC 2 cut(s) 275, 755
AxyI CCTNAGG 1 cut(s) 612
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 1062
BbvI GCAGC 1 cut(s) 1110
BccI CCATC 5 cut(s) 206, 343, 626, 1123, 1187
BceAI ACGGC 1 cut(s) 430
BcnI CCSGG 3 cut(s) 397, 462, 759
BcoDI GTCTC 1 cut(s) 680
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 3 cut(s) 23, 330, 1227
BfmI CTRYAG 1 cut(s) 390
BfrI CTTAAG 1 cut(s) 526
BisI GCNGC 1 cut(s) 1099
BlsI GCNGC 1 cut(s) 1100
Bme1390I CCNGG 3 cut(s) 397, 462, 759
Bme18I GGWCC 2 cut(s) 275, 755
BmeT110I CYCGRG 1 cut(s) 885
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 275, 755
BmiI GGNNCC 5 cut(s) 424, 609, 757, 1063, 1159
BmrFI CCNGG 3 cut(s) 397, 462, 759
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 4 cut(s) 120, 455, 560, 669
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 1062
BpuMI CCSGG 3 cut(s) 397, 462, 759
Bsa29I ATCGAT 1 cut(s) 969
BsaI GGTCTC 1 cut(s) 680
BsaJI CCNNGG 4 cut(s) 114, 396, 418, 426
BsaXI ACNNNNNCTCC 2 cut(s) 414, 444
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 432, 764, 1154
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 2 cut(s) 260, 414
Bse21I CCTNAGG 1 cut(s) 612
BseCI ATCGAT 1 cut(s) 969
BseDI CCNNGG 4 cut(s) 114, 396, 418, 426
BseGI GGATG 4 cut(s) 217, 470, 575, 1218
BseLI CCNNNNNNNGG 4 cut(s) 284, 432, 764, 1154
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 260, 414
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 1110
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 4 cut(s) 75, 239, 296, 417
BshVI ATCGAT 1 cut(s) 969
BsiEI CGRYCG 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 6 cut(s) 236, 240, 396, 462, 690, 759
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 4 cut(s) 284, 432, 764, 1154
BsmAI GTCTC 1 cut(s) 680
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 4 cut(s) 75, 239, 296, 417
Bso31I GGTCTC 1 cut(s) 680
BsoBI CYCGRG 1 cut(s) 885
Bsp1286I GDGCHC 1 cut(s) 886
Bsp143I GATC 3 cut(s) 530, 678, 970
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 2 cut(s) 658, 753
BspANI GGCC 4 cut(s) 75, 239, 296, 417
BspCNI CTCAG 1 cut(s) 284
BspDI ATCGAT 1 cut(s) 969
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 5 cut(s) 424, 609, 757, 1063, 1159
BspQI GCTCTTC 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 2 cut(s) 260, 414
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 4 cut(s) 114, 396, 418, 426
BssMI GATC 3 cut(s) 530, 678, 970
BssT1I CCWWGG 2 cut(s) 114, 418
Bst4CI ACNGT 2 cut(s) 391, 620
Bst6I CTCTTC 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 526
BstAPI GCANNNNNTGC 1 cut(s) 1113
BstC8I GCNNGC 1 cut(s) 1109
BstDEI CTNAG 2 cut(s) 271, 612
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 4 cut(s) 217, 470, 575, 1218
BstKTI GATC 3 cut(s) 533, 681, 973
BstMAI GTCTC 1 cut(s) 680
BstMBI GATC 3 cut(s) 530, 678, 970
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 2 cut(s) 1104, 1113
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 3 cut(s) 395, 460, 757
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 1110
BstV2I GAAGAC 1 cut(s) 1062
BstZI CGGCCG 1 cut(s) 237
Bsu15I ATCGAT 1 cut(s) 969
Bsu36I CCTNAGG 1 cut(s) 612
BsuRI GGCC 4 cut(s) 75, 239, 296, 417
BsuTUI ATCGAT 1 cut(s) 969
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 2 cut(s) 708, 1050
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 4 cut(s) 217, 470, 575, 1218
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 2 cut(s) 407, 1089
Cac8I GCNNGC 1 cut(s) 1109
CciI TCATGA 1 cut(s) 951
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 2 cut(s) 275, 755
ClaI ATCGAT 1 cut(s) 969
Csp6I GTAC 1 cut(s) 405
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 2 cut(s) 271, 612
DpnI GATC 3 cut(s) 532, 680, 972
DpnII GATC 3 cut(s) 530, 678, 970
EaeI YGGCCR 2 cut(s) 237, 415
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 2 cut(s) 114, 418
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 680
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 275, 755
Eco52I CGGCCG 1 cut(s) 237
Eco81I CCTNAGG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 885
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 114, 418
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 2 cut(s) 114, 418
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 1055
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 1099
FokI GGATG 4 cut(s) 224, 477, 582, 1205
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 1 cut(s) 1099
FspBI CTAG 3 cut(s) 23, 330, 1227
GluI GCNGC 1 cut(s) 1099
HaeIII GGCC 4 cut(s) 75, 239, 296, 417
HapII CCGG 6 cut(s) 236, 240, 396, 462, 690, 759
HinfI GANTC 5 cut(s) 232, 870, 890, 955, 1087
HpaII CCGG 6 cut(s) 236, 240, 396, 462, 690, 759
Hpy166II GTNNAC 2 cut(s) 110, 407
Hpy188I TCNGA 8 cut(s) 33, 352, 372, 590, 665, 987, 1049, 1092
Hpy188III TCNNGA 3 cut(s) 717, 887, 952
Hpy8I GTNNAC 2 cut(s) 110, 407
HpyAV CCTTC 2 cut(s) 143, 901
HpyCH4III ACNGT 2 cut(s) 391, 620
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 9 cut(s) 282, 450, 468, 512, 999, 1027, 1098, 1116, 1233
HpyF10VI GCNNNNNNNGC 2 cut(s) 1104, 1113
HpyF3I CTNAG 2 cut(s) 271, 612
HpySE526I ACGT 1 cut(s) 100
Kzo9I GATC 3 cut(s) 530, 678, 970
LguI GCTCTTC 1 cut(s) 211
LmnI GCTCC 4 cut(s) 140, 422, 613, 1166
Lsp1109I GCAGC 1 cut(s) 1110
LweI GCATC 4 cut(s) 120, 455, 560, 669
MaeI CTAG 3 cut(s) 23, 330, 1227
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 7 cut(s) 287, 435, 502, 1039, 1084, 1143, 1171
MalI GATC 3 cut(s) 532, 680, 972
MboI GATC 3 cut(s) 530, 678, 970
MboII GAAGA 7 cut(s) 9, 168, 228, 589, 941, 1005, 1062
MhlI GDGCHC 1 cut(s) 886
MluCI AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
MlyI GAGTC 1 cut(s) 1081
MnlI CCTC 8 cut(s) 146, 395, 436, 782, 811, 1075, 1158, 1233
MseI TTAA 5 cut(s) 386, 441, 527, 936, 1140
MslI CAYNNNNRTG 1 cut(s) 205
MspCI CTTAAG 1 cut(s) 526
MspI CCGG 6 cut(s) 236, 240, 396, 462, 690, 759
MspR9I CCNGG 3 cut(s) 397, 462, 759
MwoI GCNNNNNNNGC 2 cut(s) 1104, 1113
NciI CCSGG 3 cut(s) 397, 462, 759
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 1055
NdeII GATC 3 cut(s) 530, 678, 970
NlaIV GGNNCC 5 cut(s) 424, 609, 757, 1063, 1159
NmuCI GTSAC 3 cut(s) 435, 1084, 1171
NspI RCATGY 1 cut(s) 204
PagI TCATGA 1 cut(s) 951
PciSI GCTCTTC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 232, 870, 890, 955
PfoI TCCNGGA 2 cut(s) 460, 757
PkrI GCNGC 1 cut(s) 1100
PleI GAGTC 1 cut(s) 1081
PpsI GAGTC 1 cut(s) 1081
PspN4I GGNNCC 5 cut(s) 424, 609, 757, 1063, 1159
PspPI GGNCC 2 cut(s) 275, 755
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 5 cut(s) 386, 441, 527, 936, 1140
SatI GCNGC 1 cut(s) 1099
Sau3AI GATC 3 cut(s) 530, 678, 970
Sau96I GGNCC 2 cut(s) 275, 755
SchI GAGTC 1 cut(s) 1081
ScrFI CCNGG 3 cut(s) 397, 462, 759
SduI GDGCHC 1 cut(s) 886
SfaNI GCATC 4 cut(s) 120, 455, 560, 669
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 2 cut(s) 275, 755
SmiMI CAYNNNNRTG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 526
SmoI CTYRAG 1 cut(s) 526
SpeI ACTAGT 1 cut(s) 329
Sse9I AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
SsiI CCGC 2 cut(s) 658, 753
SspI AATATT 3 cut(s) 919, 980, 1138
SspMI CTAG 3 cut(s) 23, 330, 1227
StyD4I CCNGG 3 cut(s) 395, 460, 757
StyI CCWWGG 2 cut(s) 114, 418
TaaI ACNGT 2 cut(s) 391, 620
TaiI ACGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 485, 495, 969
TasI AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
TfiI GAWTC 4 cut(s) 232, 870, 890, 955
Tru1I TTAA 5 cut(s) 386, 441, 527, 936, 1140
Tru9I TTAA 5 cut(s) 386, 441, 527, 936, 1140
TscAI CASTG 2 cut(s) 414, 1089
TseFI GTSAC 3 cut(s) 435, 1084, 1171
TseI GCWGC 1 cut(s) 1098
Tsp45I GTSAC 3 cut(s) 435, 1084, 1171
TspDTI ATGAA 5 cut(s) 509, 554, 942, 968, 1005
TspRI CASTG 2 cut(s) 414, 1089
Vha464I CTTAAG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 275, 755
XapI RAATTY 1 cut(s) 822
XceI RCATGY 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 109
XspI CTAG 3 cut(s) 23, 330, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.