Rw5G017820

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004190
Physical Location & Seq
Forward (+)
22764756 .. 22767283
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017820.1

Sequence Viewer

Length: 1194 bp
ATGAGTTTGGTATCTTCATTCCTAGGGTTTAGGGTTTTCTTGTGTTTGTGCTTGTTGGTATGGGTAACAAAAGGCCAAAATGTCAACCTCTTTCGTCAAACCGAACAACTTGGTCGGCGCAAGATTTTCAATGTGATGGATTATGGAGCTGTTGAAGGTGGAATAACTGATAACAGCCAGGCATTTTTGAAAGCATGGAATGATGCCTGTAAATGGACTGGAAGGGCAAGGGTTTTGGTTCCCCGAGGAATATTCAAGTTATTCCCAGTTGTATTCTCAGGTCCATGCAATGGTCCGATTGCCTTTTTGATCATGGGGACTTTAAGAGCCTCTACTAATCCATCAACATTTAATTCTGAAAATTGGATAAACTTCCGATACTTGGAGCAGTTAACCGTAGCCGGAGGCGGTACCTTGGACGGCCAAGGATCCTTTGTTTGGCCTCTTAACAACTGCAACAATCGTCCATGCAAGACACTCCCCATCTCTTTGAGTTTTGACTTCGTCACAAATGCAAGCGTCGCTCACTTGAGATCATACAACAGCAAAAATGCCCATATCAAGGTTTATGGATGCAACAAAATGGACTTCACCAAACTTAGATTGTCAGCTCCAGGTGATAGCCCAAACACCGATGGCATAAAAATCGGAAACTCCTACCGCATCAGAATCTCACGCTCGGTTATTGGCACCGGCGATGACTGCATTGCGATATTGTCAGGAAGTAGGGAAATCCACATTTCTAAAATTTTTTGCGGTCCTGGGCATGGTATCAGCATTGGAAGTCTCGGAGATAGCGGTTTAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTTATTCTCACCCCTTGAAGGTTTCAAAGATTAGATACGAAAACATTGTGATGACTGATGTCACGAATCCAATTATTATCGATCAAAATTATTGCCCCAGCCCTCCCTGCAATCAACAGGTTACTTCGAATGTGCAAATTAGTGATGTGACATATAATAACATATGGGGAAGTTCTTCTTCCAAAGAAGCAGTAATTCTGAAATGCAGCAAAACCAGCCCATGCAAGAACATGGTGATGAACAATATCAAGTTATCCCTCAATGGTCCTGGAGGTATTGCGAGTTCTTCCTGCGAAAATGCCATTGGCGTCTCTTACGGCACACAAAAGCCTCCTTCTTGCATAAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

397

Amino Acids

43.23

Weight (kDa)

9.43

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 381 6.2e-89 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 410, 689
AccB7I CCANNNNNTGG 1 cut(s) 290
AciI CCGC 4 cut(s) 408, 661, 756, 798
AclWI GGATC 2 cut(s) 423, 436
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 1149
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 7 cut(s) 213, 290, 382, 438, 562, 767, 859
AgsI TTSAA 6 cut(s) 130, 155, 190, 256, 859, 867
AjnI CCWGG 4 cut(s) 177, 613, 760, 1108
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
AluBI AGCT 2 cut(s) 149, 611
AluI AGCT 2 cut(s) 149, 611
Alw26I GTCTC 2 cut(s) 791, 1156
AlwI GGATC 2 cut(s) 423, 436
Ama87I CYCGRG 1 cut(s) 243
AoxI GGCC 3 cut(s) 73, 421, 440
ApeKI GCWGC 1 cut(s) 1047
ApoI RAATTY 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 1015
Asp718I GGTACC 1 cut(s) 410
AspA2I CCTAGG 1 cut(s) 22
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 4 cut(s) 281, 293, 758, 1106
AsuHPI GGTGA 4 cut(s) 583, 629, 842, 1087
AsuII TTCGAA 1 cut(s) 968
AvaI CYCGRG 1 cut(s) 243
AvaII GGWCC 4 cut(s) 281, 293, 758, 1106
AvrII CCTAGG 1 cut(s) 22
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 2 cut(s) 410, 689
BbvI GCAGC 1 cut(s) 1059
BccI CCATC 4 cut(s) 130, 349, 491, 629
BceAI ACGGC 2 cut(s) 436, 1174
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 4 cut(s) 179, 615, 762, 1110
BclI TGATCA 1 cut(s) 309
BcoDI GTCTC 2 cut(s) 791, 1156
BfaI CTAG 2 cut(s) 23, 1192
BisI GCNGC 1 cut(s) 1048
BlnI CCTAGG 1 cut(s) 22
BlsI GCNGC 1 cut(s) 1049
Bme1390I CCNGG 4 cut(s) 179, 615, 762, 1110
Bme18I GGWCC 4 cut(s) 281, 293, 758, 1106
BmeT110I CYCGRG 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 281, 293, 758, 1106
BmiI GGNNCC 4 cut(s) 240, 412, 430, 691
BmrFI CCNGG 4 cut(s) 179, 615, 762, 1110
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 3 cut(s) 193, 563, 672
BmuI ACTGGG 1 cut(s) 260
BoxI GACNNNNGTC 1 cut(s) 899
BpmI CTGGAG 2 cut(s) 597, 1131
Bpu14I TTCGAA 1 cut(s) 968
BpuEI CTTGAG 1 cut(s) 550
Bsa29I ATCGAT 1 cut(s) 921
BsaHI GRCGYC 1 cut(s) 1149
BsaJI CCNNGG 5 cut(s) 22, 244, 414, 424, 761
Bsc4I CCNNNNNNNGG 7 cut(s) 213, 290, 382, 438, 562, 767, 859
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 2 cut(s) 223, 266
Bse3DI GCAATG 2 cut(s) 295, 705
BseBI CCWGG 4 cut(s) 179, 615, 762, 1110
BseCI ATCGAT 1 cut(s) 921
BseDI CCNNGG 5 cut(s) 22, 244, 414, 424, 761
BseGI GGATG 1 cut(s) 578
BseLI CCNNNNNNNGG 7 cut(s) 213, 290, 382, 438, 562, 767, 859
BseMI GCAATG 2 cut(s) 295, 705
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 223, 266
BseXI GCAGC 1 cut(s) 1059
BseYI CCCAGC 1 cut(s) 938
BshFI GGCC 3 cut(s) 75, 423, 442
BshNI GGYRCC 2 cut(s) 410, 689
BshVI ATCGAT 1 cut(s) 921
BsiHKCI CYCGRG 1 cut(s) 243
BsiSI CCGG 2 cut(s) 402, 693
BslFI GGGAC 2 cut(s) 331, 823
BslI CCNNNNNNNGG 7 cut(s) 213, 290, 382, 438, 562, 767, 859
BsmAI GTCTC 2 cut(s) 791, 1156
BsmBI CGTCTC 1 cut(s) 1156
BsmFI GGGAC 2 cut(s) 331, 823
BsnI GGCC 3 cut(s) 75, 423, 442
BsoBI CYCGRG 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 968
Bsp143I GATC 4 cut(s) 309, 428, 533, 922
BspACI CCGC 4 cut(s) 408, 661, 756, 798
BspANI GGCC 3 cut(s) 75, 423, 442
BspCNI CTCAG 1 cut(s) 290
BspDI ATCGAT 1 cut(s) 921
BspLI GGNNCC 4 cut(s) 240, 412, 430, 691
BspPI GGATC 2 cut(s) 423, 436
BspT104I TTCGAA 1 cut(s) 968
BspT107I GGYRCC 2 cut(s) 410, 689
BsrDI GCAATG 2 cut(s) 295, 705
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 2 cut(s) 223, 266
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 5 cut(s) 22, 244, 414, 424, 761
BssMI GATC 4 cut(s) 309, 428, 533, 922
BssNI GRCGYC 1 cut(s) 1149
BssT1I CCWWGG 3 cut(s) 22, 414, 424
Bst2UI CCWGG 4 cut(s) 179, 615, 762, 1110
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 968
BstC8I GCNNGC 1 cut(s) 517
BstDEI CTNAG 3 cut(s) 277, 599, 824
BstF5I GGATG 1 cut(s) 578
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 4 cut(s) 312, 431, 536, 925
BstMAI GTCTC 2 cut(s) 791, 1156
BstMBI GATC 4 cut(s) 309, 428, 533, 922
BstMWI GCNNNNNNNGC 4 cut(s) 521, 702, 948, 1056
BstNI CCWGG 4 cut(s) 179, 615, 762, 1110
BstPAI GACNNNNGTC 1 cut(s) 899
BstSCI CCNGG 4 cut(s) 177, 613, 760, 1108
BstV1I GCAGC 1 cut(s) 1059
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
Bsu15I ATCGAT 1 cut(s) 921
BsuRI GGCC 3 cut(s) 75, 423, 442
BsuTUI ATCGAT 1 cut(s) 921
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 1 cut(s) 578
Cac8I GCNNGC 1 cut(s) 517
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 4 cut(s) 281, 293, 758, 1106
ClaI ATCGAT 1 cut(s) 921
CseI GACGC 2 cut(s) 508, 1138
Csp6I GTAC 1 cut(s) 411
CviAII CATG 8 cut(s) 195, 285, 313, 468, 767, 837, 1062, 1072
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 277, 599, 824
DpnI GATC 4 cut(s) 311, 430, 535, 924
DpnII GATC 4 cut(s) 309, 428, 533, 922
DraI TTTAAA 1 cut(s) 804
EaeI YGGCCR 1 cut(s) 421
Eco130I CCWWGG 3 cut(s) 22, 414, 424
Eco47I GGWCC 4 cut(s) 281, 293, 758, 1106
Eco88I CYCGRG 1 cut(s) 243
EcoRII CCWGG 4 cut(s) 177, 613, 760, 1108
EcoT14I CCWWGG 3 cut(s) 22, 414, 424
ErhI CCWWGG 3 cut(s) 22, 414, 424
Esp3I CGTCTC 1 cut(s) 1156
FaeI CATG 8 cut(s) 198, 288, 316, 471, 770, 840, 1065, 1075
FalI AAGNNNNNCTT 2 cut(s) 1003, 1035
FaqI GGGAC 2 cut(s) 331, 823
FatI CATG 8 cut(s) 194, 284, 312, 467, 766, 836, 1061, 1071
FauNDI CATATG 1 cut(s) 1004
FbaI TGATCA 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 1048
FokI GGATG 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 1048
FspBI CTAG 2 cut(s) 23, 1192
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 1048
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 2 cut(s) 597, 1131
HaeIII GGCC 3 cut(s) 75, 423, 442
HapII CCGG 2 cut(s) 402, 693
HgaI GACGC 2 cut(s) 508, 1138
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 1149
Hin1II CATG 8 cut(s) 198, 288, 316, 471, 770, 840, 1065, 1075
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 2 cut(s) 85, 393
HindII GTYRAC 2 cut(s) 85, 393
HinfI GANTC 3 cut(s) 669, 828, 907
HpaI GTTAAC 1 cut(s) 393
HpaII CCGG 2 cut(s) 402, 693
HphI GGTGA 4 cut(s) 583, 629, 842, 1087
Hpy166II GTNNAC 2 cut(s) 85, 393
Hpy188I TCNGA 7 cut(s) 297, 358, 377, 650, 668, 791, 1041
Hpy188III TCNNGA 2 cut(s) 720, 904
Hpy8I GTNNAC 2 cut(s) 85, 393
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 4 cut(s) 149, 216, 853, 1185
HpyCH4III ACNGT 1 cut(s) 397
HpyF10VI GCNNNNNNNGC 4 cut(s) 521, 702, 948, 1056
HpyF3I CTNAG 3 cut(s) 277, 599, 824
Hsp92I GRCGYC 1 cut(s) 1149
Hsp92II CATG 8 cut(s) 198, 288, 316, 471, 770, 840, 1065, 1075
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 309
KspAI GTTAAC 1 cut(s) 393
Kzo9I GATC 4 cut(s) 309, 428, 533, 922
LmnI GCTCC 3 cut(s) 146, 385, 616
Lsp1109I GCAGC 1 cut(s) 1059
LweI GCATC 3 cut(s) 193, 563, 672
MaeI CTAG 2 cut(s) 23, 1192
MaeIII GTNAC 5 cut(s) 64, 505, 901, 961, 988
MalI GATC 4 cut(s) 311, 430, 535, 924
MboI GATC 4 cut(s) 309, 428, 533, 922
MboII GAAGA 4 cut(s) 6, 1008, 1011, 1119
MflI RGATCY 1 cut(s) 428
MluCI AATT 7 cut(s) 352, 361, 747, 912, 928, 978, 1035
MnlI CCTC 9 cut(s) 98, 239, 340, 398, 453, 954, 1106, 1109, 1182
MroXI GAANNNNTTC 1 cut(s) 1015
MseI TTAA 5 cut(s) 323, 351, 392, 447, 803
MslI CAYNNNNRTG 2 cut(s) 890, 1076
MspI CCGG 2 cut(s) 402, 693
MspR9I CCNGG 4 cut(s) 179, 615, 762, 1110
MvaI CCWGG 4 cut(s) 179, 615, 762, 1110
MwoI GCNNNNNNNGC 4 cut(s) 521, 702, 948, 1056
NdeI CATATG 1 cut(s) 1004
NdeII GATC 4 cut(s) 309, 428, 533, 922
NlaIII CATG 8 cut(s) 198, 288, 316, 471, 770, 840, 1065, 1075
NlaIV GGNNCC 4 cut(s) 240, 412, 430, 691
NmuCI GTSAC 3 cut(s) 505, 901, 988
NspV TTCGAA 1 cut(s) 968
PdmI GAANNNNTTC 1 cut(s) 1015
PfeI GAWTC 3 cut(s) 669, 828, 907
PflFI GACNNNGTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 1 cut(s) 1108
PkrI GCNGC 1 cut(s) 1049
PshAI GACNNNNGTC 1 cut(s) 899
Psp6I CCWGG 4 cut(s) 177, 613, 760, 1108
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 4 cut(s) 177, 613, 760, 1108
PspN4I GGNNCC 4 cut(s) 240, 412, 430, 691
PspPI GGNCC 4 cut(s) 281, 293, 758, 1106
PsrI GAACNNNNNNTAC 2 cut(s) 1108, 1140
PsuI RGATCY 1 cut(s) 428
PsyI GACNNNGTC 1 cut(s) 503
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
RseI CAYNNNNRTG 2 cut(s) 890, 1076
SaqAI TTAA 5 cut(s) 323, 351, 392, 447, 803
SatI GCNGC 1 cut(s) 1048
Sau3AI GATC 4 cut(s) 309, 428, 533, 922
Sau96I GGNCC 4 cut(s) 281, 293, 758, 1106
ScrFI CCNGG 4 cut(s) 179, 615, 762, 1110
SfaNI GCATC 3 cut(s) 193, 563, 672
SfuI TTCGAA 1 cut(s) 968
SgrAI CRCCGGYG 1 cut(s) 692
SinI GGWCC 4 cut(s) 281, 293, 758, 1106
SmiMI CAYNNNNRTG 2 cut(s) 890, 1076
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 7 cut(s) 352, 361, 747, 912, 928, 978, 1035
SsiI CCGC 4 cut(s) 408, 661, 756, 798
SspI AATATT 1 cut(s) 252
SspMI CTAG 2 cut(s) 23, 1192
StyD4I CCNGG 4 cut(s) 177, 613, 760, 1108
StyI CCWWGG 3 cut(s) 22, 414, 424
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 2 cut(s) 921, 968
TasI AATT 7 cut(s) 352, 361, 747, 912, 928, 978, 1035
TfiI GAWTC 3 cut(s) 669, 828, 907
Tru1I TTAA 5 cut(s) 323, 351, 392, 447, 803
Tru9I TTAA 5 cut(s) 323, 351, 392, 447, 803
TseFI GTSAC 3 cut(s) 505, 901, 988
TseI GCWGC 1 cut(s) 1047
Tsp45I GTSAC 3 cut(s) 505, 901, 988
TspDTI ATGAA 2 cut(s) 6, 1094
Tth111I GACNNNGTC 1 cut(s) 503
Van91I CCANNNNNTGG 1 cut(s) 290
VpaK11BI GGWCC 4 cut(s) 281, 293, 758, 1106
XapI RAATTY 1 cut(s) 747
XmaJI CCTAGG 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 1015
XspI CTAG 2 cut(s) 23, 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.