FvH4_6g48250

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004190
Physical Location & Seq
Forward (+)
36375391 .. 36377176
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48250.t1

Sequence Viewer

Length: 1230 bp
ATGAGTTTGATATCTTCATTTCTAGGGTTTAGGGTTTTCTTGTTCATGTTCTTGTTGGTATGGGTAACAAATGGCCAGAATGTTAACCTCTTTCGTCAAACCGAACAACTAGGTCGGCGCAAGTTCTTCAATGTGATGGATTATGGAGCTGTTGAAGGTGGAGTAACTGATAACAGTCAGGCATTCTTGAAAGCATGGAAGGATGCGTGTCAATGGAAAGGAAGGGCAAAGGTTTTGATTCCCAGAGGAATATTCAAGTTGTTCCCAGTTGTATTCTCAGGTCCCTGCAATGGTCCGATTGCCTTTTTGATTATGGGGACTTTAAGAGCCTCGACTGATCCATCAACATTCAACTCTGAAAACTGGATAAACTTCCGATACTTGGAGCAGTTAACCTTAGCCGGAGGCGGTACCTTGGACGGCCAAGGATCCTTAGTTTGGCCTCACAACAACTGCAACAATGGTCCATGCAGGGCACTCCCCATCTCTTTGAGTTTTTACTTCGTCACAAATGCAACCGTCGCTCACTTGAGATCATACAACAGCAAAATGGCTCATATTAAGGTTTATGGATGCAACAAAATGGACTTCACCAAACTTAGATTGTCAGCTCCTGGTGATAGCCCCAACACCGATGGCATAAAAATCGGAAACTCCTACCGTATCAGAATCTCACGTTCGGTTATTAGCACCGGCGATGACTGCATTGCCATATTGGCAGGAAGTAGGGAGATCCACATTTCTAAGATTTTCTGCGGTCCGGGGCATGGAATCAGCATTGGAAGTCTGGGAGGGTATGGTAATGAAAATGATGTGGAAGGAGTATATGTGAAAGATAGTGGCTTAAAGGGGACTACCAATGGTCTAAGAATCAAAACATGGGCTAAATCTCATCCCTTGAAGGTTTCAAAGATTATATATGATAACATTGTGATGACTGATGTCATGAACCCAATTATTATCGATCAGAATTATTGCCCTAACCCTCCCTGCAATCAACAGGTTACTTCGAATGTGCAGATTAGTGGTGTGACATATAACAACATACGGGGAACTTCTTCCTCCAAAGAAGCAGTGATTCTGAAATGCAGCAGAACCAGCCCTTGCAAGAACATAGTGATGAACAATATCAAGTTATCCCTCAACGGTACCGGAGGGTTTGCGAGTTCTTACTGTGAAAATGCCGTTGGTGTATCTTATGGCACACAAAACCCTCGTCCTTGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

410

Amino Acids

44.74

Weight (kDa)

9.38

Isoelectric Point (pI)

25.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 395 1.2e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 410, 1148
AccB1I GGYRCC 2 cut(s) 410, 1148
AciI CCGC 2 cut(s) 408, 756
AclWI GGATC 4 cut(s) 332, 423, 436, 727
AcoI YGGCCR 2 cut(s) 73, 421
AfaI GTAC 2 cut(s) 412, 1150
AfiI CCNNNNNNNGG 4 cut(s) 290, 382, 438, 767
AgsI TTSAA 7 cut(s) 130, 155, 190, 256, 352, 901, 909
AjnI CCWGG 1 cut(s) 613
AjuI GAANNNNNNNTTGG 2 cut(s) 1170, 1202
AluBI AGCT 2 cut(s) 149, 611
AluI AGCT 2 cut(s) 149, 611
AlwI GGATC 4 cut(s) 332, 423, 436, 727
AlwNI CAGNNNCTG 1 cut(s) 614
AoxI GGCC 3 cut(s) 73, 421, 440
ApeKI GCWGC 1 cut(s) 1089
ArsI GACNNNNNNTTYG 1 cut(s) 1201
Asp700I GAANNNNTTC 1 cut(s) 1057
Asp718I GGTACC 2 cut(s) 410, 1148
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 4 cut(s) 281, 293, 464, 758
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 2 cut(s) 583, 629
AsuII TTCGAA 1 cut(s) 1010
AvaII GGWCC 4 cut(s) 281, 293, 464, 758
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 1 cut(s) 75
BamHI GGATCC 1 cut(s) 428
BanI GGYRCC 2 cut(s) 410, 1148
BbvI GCAGC 1 cut(s) 1101
BccI CCATC 4 cut(s) 130, 349, 491, 629
BceAI ACGGC 2 cut(s) 436, 1169
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BciT130I CCWGG 1 cut(s) 615
BcnI CCSGG 1 cut(s) 762
BfaI CTAG 2 cut(s) 23, 110
BglI GCCNNNNNGGC 1 cut(s) 716
BisI GCNGC 1 cut(s) 1090
BlsI GCNGC 1 cut(s) 1091
Bme1390I CCNGG 2 cut(s) 615, 762
Bme18I GGWCC 4 cut(s) 281, 293, 464, 758
BmgT120I GGNCC 4 cut(s) 281, 293, 464, 758
BmiI GGNNCC 4 cut(s) 283, 412, 430, 1150
BmrFI CCNGG 2 cut(s) 615, 762
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 193, 563
BmuI ACTGGG 1 cut(s) 260
BoxI GACNNNNGTC 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 397
Bpu14I TTCGAA 1 cut(s) 1010
BpuEI CTTGAG 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 963
BsaJI CCNNGG 3 cut(s) 414, 424, 761
BsaWI WCCGGW 1 cut(s) 1151
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 382, 438, 767
Bse118I RCCGGY 1 cut(s) 692
Bse1I ACTGG 2 cut(s) 266, 368
Bse3DI GCAATG 2 cut(s) 295, 705
BseBI CCWGG 1 cut(s) 615
BseCI ATCGAT 1 cut(s) 963
BseDI CCNNGG 3 cut(s) 414, 424, 761
BseGI GGATG 3 cut(s) 208, 578, 892
BseLI CCNNNNNNNGG 4 cut(s) 290, 382, 438, 767
BseMI GCAATG 2 cut(s) 295, 705
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 2 cut(s) 266, 368
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 1 cut(s) 1101
BsgI GTGCAG 1 cut(s) 1037
BshFI GGCC 3 cut(s) 75, 423, 442
BshNI GGYRCC 2 cut(s) 410, 1148
BshVI ATCGAT 1 cut(s) 963
BsiSI CCGG 4 cut(s) 402, 693, 761, 1152
BslFI GGGAC 3 cut(s) 267, 331, 865
BslI CCNNNNNNNGG 4 cut(s) 290, 382, 438, 767
BsmFI GGGAC 3 cut(s) 267, 331, 865
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 3 cut(s) 75, 423, 442
Bsp119I TTCGAA 1 cut(s) 1010
Bsp1286I GDGCHC 1 cut(s) 478
Bsp143I GATC 5 cut(s) 337, 428, 533, 732, 964
BspACI CCGC 2 cut(s) 408, 756
BspANI GGCC 3 cut(s) 75, 423, 442
BspCNI CTCAG 1 cut(s) 290
BspDI ATCGAT 1 cut(s) 963
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 4 cut(s) 283, 412, 430, 1150
BspPI GGATC 4 cut(s) 332, 423, 436, 727
BspT104I TTCGAA 1 cut(s) 1010
BspT107I GGYRCC 2 cut(s) 410, 1148
BsrDI GCAATG 2 cut(s) 295, 705
BsrFI RCCGGY 1 cut(s) 692
BsrI ACTGG 2 cut(s) 266, 368
BssAI RCCGGY 1 cut(s) 692
BssECI CCNNGG 3 cut(s) 414, 424, 761
BssMI GATC 5 cut(s) 337, 428, 533, 732, 964
BssT1I CCWWGG 2 cut(s) 414, 424
Bst2UI CCWGG 1 cut(s) 615
Bst4CI ACNGT 5 cut(s) 176, 520, 662, 1148, 1175
BstBI TTCGAA 1 cut(s) 1010
BstDEI CTNAG 6 cut(s) 277, 397, 433, 599, 744, 866
BstF5I GGATG 3 cut(s) 208, 578, 892
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 5 cut(s) 340, 431, 536, 735, 967
BstMBI GATC 5 cut(s) 337, 428, 533, 732, 964
BstMWI GCNNNNNNNGC 4 cut(s) 521, 702, 716, 1098
BstNI CCWGG 1 cut(s) 615
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 2 cut(s) 613, 760
BstSLI GKGCMC 1 cut(s) 478
BstV1I GCAGC 1 cut(s) 1101
BstX2I RGATCY 2 cut(s) 428, 732
BstYI RGATCY 2 cut(s) 428, 732
Bsu15I ATCGAT 1 cut(s) 963
BsuRI GGCC 3 cut(s) 75, 423, 442
BsuTUI ATCGAT 1 cut(s) 963
BtgZI GCGATG 1 cut(s) 711
BtsCI GGATG 3 cut(s) 208, 578, 892
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 1 cut(s) 1080
CaiI CAGNNNCTG 1 cut(s) 614
CciI TCATGA 1 cut(s) 945
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 692
Cfr13I GGNCC 4 cut(s) 281, 293, 464, 758
ClaI ATCGAT 1 cut(s) 963
CpoI CGGWCCG 1 cut(s) 758
Csp6I GTAC 2 cut(s) 411, 1149
CspI CGGWCCG 1 cut(s) 758
CviAII CATG 6 cut(s) 46, 195, 468, 767, 879, 946
CviQI GTAC 2 cut(s) 411, 1149
DdeI CTNAG 6 cut(s) 277, 397, 433, 599, 744, 866
DpnI GATC 5 cut(s) 339, 430, 535, 734, 966
DpnII GATC 5 cut(s) 337, 428, 533, 732, 964
EaeI YGGCCR 2 cut(s) 73, 421
Eco130I CCWWGG 2 cut(s) 414, 424
Eco32I GATATC 1 cut(s) 12
Eco47I GGWCC 4 cut(s) 281, 293, 464, 758
EcoO109I RGGNCCY 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 613
EcoRV GATATC 1 cut(s) 12
EcoT14I CCWWGG 2 cut(s) 414, 424
ErhI CCWWGG 2 cut(s) 414, 424
FaeI CATG 6 cut(s) 49, 198, 471, 770, 882, 949
FaqI GGGAC 3 cut(s) 267, 331, 865
FatI CATG 6 cut(s) 45, 194, 467, 766, 878, 945
Fnu4HI GCNGC 1 cut(s) 1090
FokI GGATG 3 cut(s) 215, 585, 879
Fsp4HI GCNGC 1 cut(s) 1090
FspBI CTAG 2 cut(s) 23, 110
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 1090
HaeIII GGCC 3 cut(s) 75, 423, 442
HapII CCGG 4 cut(s) 402, 693, 761, 1152
HhaI GCGC 1 cut(s) 120
Hin1II CATG 6 cut(s) 49, 198, 471, 770, 882, 949
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 2 cut(s) 85, 393
HindII GTYRAC 2 cut(s) 85, 393
HinfI GANTC 5 cut(s) 238, 669, 771, 870, 1078
HpaI GTTAAC 2 cut(s) 85, 393
HpaII CCGG 4 cut(s) 402, 693, 761, 1152
HphI GGTGA 2 cut(s) 583, 629
Hpy166II GTNNAC 2 cut(s) 85, 393
Hpy188I TCNGA 7 cut(s) 297, 358, 377, 650, 668, 969, 1083
Hpy188III TCNNGA 2 cut(s) 187, 946
Hpy8I GTNNAC 2 cut(s) 85, 393
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 5 cut(s) 149, 193, 216, 812, 895
HpyCH4III ACNGT 5 cut(s) 176, 520, 662, 1148, 1175
HpyCH4IV ACGT 1 cut(s) 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 521, 702, 716, 1098
HpyF3I CTNAG 6 cut(s) 277, 397, 433, 599, 744, 866
HpySE526I ACGT 1 cut(s) 676
Hsp92II CATG 6 cut(s) 49, 198, 471, 770, 882, 949
HspAI GCGC 1 cut(s) 118
KpnI GGTACC 2 cut(s) 414, 1152
KspAI GTTAAC 2 cut(s) 85, 393
Kzo9I GATC 5 cut(s) 337, 428, 533, 732, 964
LmnI GCTCC 3 cut(s) 146, 385, 616
Lsp1109I GCAGC 1 cut(s) 1101
LweI GCATC 2 cut(s) 193, 563
MaeI CTAG 2 cut(s) 23, 110
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 5 cut(s) 64, 163, 505, 1003, 1030
MalI GATC 5 cut(s) 339, 430, 535, 734, 966
MboI GATC 5 cut(s) 337, 428, 533, 732, 964
MboII GAAGA 3 cut(s) 6, 118, 1050
MflI RGATCY 2 cut(s) 428, 732
MhlI GDGCHC 1 cut(s) 478
MlsI TGGCCA 1 cut(s) 75
MluCI AATT 2 cut(s) 954, 970
MluNI TGGCCA 1 cut(s) 75
Mox20I TGGCCA 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 1057
MscI TGGCCA 1 cut(s) 75
MseI TTAA 5 cut(s) 84, 323, 392, 561, 845
MslI CAYNNNNRTG 2 cut(s) 932, 1118
Msp20I TGGCCA 1 cut(s) 75
MspI CCGG 4 cut(s) 402, 693, 761, 1152
MspR9I CCNGG 2 cut(s) 615, 762
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 615
MwoI GCNNNNNNNGC 4 cut(s) 521, 702, 716, 1098
NciI CCSGG 1 cut(s) 762
NdeII GATC 5 cut(s) 337, 428, 533, 732, 964
NlaIII CATG 6 cut(s) 49, 198, 471, 770, 882, 949
NlaIV GGNNCC 4 cut(s) 283, 412, 430, 1150
NmuCI GTSAC 2 cut(s) 505, 1030
NspV TTCGAA 1 cut(s) 1010
PagI TCATGA 1 cut(s) 945
PctI GAATGC 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 1057
PfeI GAWTC 5 cut(s) 238, 669, 771, 870, 1078
PkrI GCNGC 1 cut(s) 1091
PpuMI RGGWCCY 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 281
Psp6I CCWGG 1 cut(s) 613
PspGI CCWGG 1 cut(s) 613
PspN4I GGNNCC 4 cut(s) 283, 412, 430, 1150
PspPI GGNCC 4 cut(s) 281, 293, 464, 758
PspPPI RGGWCCY 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 2 cut(s) 428, 732
RsaI GTAC 2 cut(s) 412, 1150
RsaNI GTAC 2 cut(s) 411, 1149
RseI CAYNNNNRTG 2 cut(s) 932, 1118
Rsr2I CGGWCCG 1 cut(s) 758
RsrII CGGWCCG 1 cut(s) 758
SaqAI TTAA 5 cut(s) 84, 323, 392, 561, 845
SatI GCNGC 1 cut(s) 1090
Sau3AI GATC 5 cut(s) 337, 428, 533, 732, 964
Sau96I GGNCC 4 cut(s) 281, 293, 464, 758
ScrFI CCNGG 2 cut(s) 615, 762
SduI GDGCHC 1 cut(s) 478
SfaNI GCATC 2 cut(s) 193, 563
SfuI TTCGAA 1 cut(s) 1010
SgrAI CRCCGGYG 1 cut(s) 692
SinI GGWCC 4 cut(s) 281, 293, 464, 758
SmiMI CAYNNNNRTG 2 cut(s) 932, 1118
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 2 cut(s) 954, 970
SsiI CCGC 2 cut(s) 408, 756
SspI AATATT 1 cut(s) 252
SspMI CTAG 2 cut(s) 23, 110
StyD4I CCNGG 2 cut(s) 613, 760
StyI CCWWGG 2 cut(s) 414, 424
TaaI ACNGT 5 cut(s) 176, 520, 662, 1148, 1175
TaiI ACGT 1 cut(s) 679
TaqI TCGA 3 cut(s) 332, 963, 1010
TasI AATT 2 cut(s) 954, 970
TfiI GAWTC 5 cut(s) 238, 669, 771, 870, 1078
Tru1I TTAA 5 cut(s) 84, 323, 392, 561, 845
Tru9I TTAA 5 cut(s) 84, 323, 392, 561, 845
TscAI CASTG 1 cut(s) 1080
TseFI GTSAC 2 cut(s) 505, 1030
TseI GCWGC 1 cut(s) 1089
Tsp45I GTSAC 2 cut(s) 505, 1030
TspDTI ATGAA 5 cut(s) 6, 34, 819, 962, 1136
TspRI CASTG 1 cut(s) 1080
VpaK11BI GGWCC 4 cut(s) 281, 293, 464, 758
XmnI GAANNNNTTC 1 cut(s) 1057
XspI CTAG 2 cut(s) 23, 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.