Rh6DG027300

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0004190
Physical Location & Seq
Reverse (-)
2401722 .. 2402229
508 bp
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UTR
Exon/CDS
Intron
Rh6DG027300.1

Sequence Viewer

Length: 378 bp
ATGGTTTTCAATGTGAAGCGTTATGGAGCTGTTGAAGGAGGAAGAACTGATAACAGCCAGGCATTTTTGAAAGCATGGAGTGAAGCATGTAAATGGGATGGAAGAGCAAAGGTTGAAATTCCGGCAGGAACATTCAAGTTGTTCCCAATTGTATTCTCAAGTCCATGCAAGGGTCGCATGGCCTCCGTAATCAAGGGAACTTTACTGGTTTCTACTAGTCCATCAGCATTGAGTTCTGAAAATTGGATAAACTTTCGGAGCATTGAACAACTGACCGTAGCCGGAGGTACGCTAGATGGCCAAGGAGCTTCGGTTTGGTCTCTTAACGATTGCAAAACTAATCCCAGATGCAAAGCTCTCCCAATTGTAAGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

125

Amino Acids

13.44

Weight (kDa)

9.42

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 30 - 122 1.3e-12 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 1 cut(s) 117
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 6 cut(s) 10, 35, 70, 116, 136, 266
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 1 cut(s) 57
AjuI GAANNNNNNNTTGG 1 cut(s) 355
AluBI AGCT 3 cut(s) 29, 308, 356
AluI AGCT 3 cut(s) 29, 308, 356
Alw26I GTCTC 1 cut(s) 324
AoxI GGCC 2 cut(s) 180, 298
ApoI RAATTY 1 cut(s) 117
BalI TGGCCA 1 cut(s) 300
BccI CCATC 3 cut(s) 92, 229, 290
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 324
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 2 cut(s) 216, 293
Bme1390I CCNGG 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 142
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 170
BseNI ACTGG 1 cut(s) 210
BshFI GGCC 2 cut(s) 182, 300
BsiSI CCGG 2 cut(s) 122, 282
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 324
BsnI GGCC 2 cut(s) 182, 300
Bso31I GGTCTC 1 cut(s) 324
BspANI GGCC 2 cut(s) 182, 300
BspQI GCTCTTC 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 324
BsrI ACTGG 1 cut(s) 210
BssECI CCNNGG 1 cut(s) 301
BssT1I CCWWGG 1 cut(s) 301
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 324
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 182, 300
BtsCI GGATG 1 cut(s) 103
Csp6I GTAC 1 cut(s) 288
CviAII CATG 4 cut(s) 75, 87, 165, 178
CviJI RGCY 7 cut(s) 29, 57, 182, 281, 300, 308, 356
CviKI_1 RGCY 7 cut(s) 29, 57, 182, 281, 300, 308, 356
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 4 cut(s) 78, 90, 168, 181
FaiI YATR 5 cut(s) 24, 76, 88, 166, 179
FatI CATG 4 cut(s) 74, 86, 164, 177
FokI GGATG 1 cut(s) 110
FspBI CTAG 2 cut(s) 216, 293
HaeIII GGCC 2 cut(s) 182, 300
HapII CCGG 2 cut(s) 122, 282
Hin1II CATG 4 cut(s) 78, 90, 168, 181
HpaII CCGG 2 cut(s) 122, 282
Hpy188I TCNGA 2 cut(s) 238, 258
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4V TGCA 3 cut(s) 168, 333, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 4 cut(s) 78, 90, 168, 181
LguI GCTCTTC 1 cut(s) 97
LmnI GCTCC 3 cut(s) 26, 258, 305
LpnPI CCDG 7 cut(s) 44, 71, 111, 135, 191, 295, 358
LweI GCATC 1 cut(s) 338
MaeI CTAG 2 cut(s) 216, 293
MboII GAAGA 2 cut(s) 54, 114
MfeI CAATTG 2 cut(s) 147, 363
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 4 cut(s) 117, 147, 241, 363
MluNI TGGCCA 1 cut(s) 300
MnlI CCTC 3 cut(s) 32, 193, 278
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 91
Msp20I TGGCCA 1 cut(s) 300
MspI CCGG 2 cut(s) 122, 282
MspR9I CCNGG 1 cut(s) 59
MunI CAATTG 2 cut(s) 147, 363
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 1 cut(s) 174
NlaIII CATG 4 cut(s) 78, 90, 168, 181
NspI RCATGY 1 cut(s) 90
PciSI GCTCTTC 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
RseI CAYNNNNRTG 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 59
SetI ASST 5 cut(s) 31, 114, 289, 310, 358
SfaNI GCATC 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 4 cut(s) 117, 147, 241, 363
SspMI CTAG 2 cut(s) 216, 293
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 277
TasI AATT 4 cut(s) 117, 147, 241, 363
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TspGWI ACGGA 1 cut(s) 175
XapI RAATTY 1 cut(s) 117
XceI RCATGY 1 cut(s) 90
XspI CTAG 2 cut(s) 216, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.