Rw5G001770

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004190
Physical Location & Seq
Reverse (-)
1535586 .. 1537602
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001770.1

Sequence Viewer

Length: 1242 bp
ATGATTGTTTTGATATCTTCCCTAGTCTTTCGGACTTTCTTGTTTTTGTTCTTGTTGGCATGGATAACAGAAGGCCACAAACTCTATCGTCAAACAGCACGTCTTGGTAGACCCATGGTTTTCAATGTGATGCGTTATGGAGCTATTGAAGGAGGAAGAACTGATAATAGTCAGGCATTTTTGAAAGCATGGAGTGAAGCATGTAAATGGGATGGAAGAGCAAAGGTTGTGATTCCGCCCGGAACATTCAAGTTGTTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTAACATGGCCTTTGTAATCAAGGGAACTTTGTTGGCTTCTACTAGTCCATCAGCATTGAGTTCTGAAAATTGGATAAACTTTCGGAACATTGAACAATTAACTGTAGCCGGGGGAGGTACGCTGGACGGCCAAGGATCCTCGGTTTGGTCTCTTAACGATTGCAAAACTAATCCCGTATGCAAAGCTCTCCCAACTTCAATGACTTTCGACTTCGTAACAAATGCAAGTGTTCATCACTTAAGATCATTCAACAGCCAAAATGGTCATTTTAAGGTTCATGGATGCCAGAAGATAGACTTCGGAAACATTAGAATATCGGCTCCTGAGGACAGTCCAAACACCGATGGCATAAAAATTGGTAACTCATACCGCATCAGAATTAGACGAACGATCATTGAGACCGGCGATGATTGTATAGGAATTGTATCGGGAAGTCAAAAAATCCACATTTCTAATGTCTTTTGCGGTCCCGGACATGGAATTAGCATTGGAAGTCTTGGAGGGCATAGTTATACAGAATATGATGTGGAGGGAATTTTTGTGAAAAATAGCGTTTTCAAAAAGACTGATAATGGTTTGAGAATCAAAACATGGGCTCGAGATACTCCAAAACCATTGAAGGCTTCAAATATTGTGTATGAAGATATTAACATGATTGATGTCATGAATCCCATTATCATCGATCAACAATATTGTCCGAACAATCAATGCAATCAACAGGATACTTCATCAAATGTGCAAATCAGCGATGTAACATTCAGAAACATATGGGGAACCTCGTCTTCCCAAGAAGCAGTGACTCTGAAATGCAGCAAAAGCAAGCCATGCAAGAATATAGTGATGGAGAATATCAAGTTACCTCATACTGATTCCAAAGGAGCTGCGACGACTTCCATCTGTAATAATGTCATCGGCATCTCTTATGGCTCACACATCCCTCCTAGTTGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

413

Amino Acids

45.33

Weight (kDa)

9.04

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 399 9.4e-94 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 109
AciI CCGC 3 cut(s) 236, 658, 753
AclWI GGATC 2 cut(s) 417, 430
AcoI YGGCCR 1 cut(s) 415
AcsI RAATTY 1 cut(s) 822
AfaI GTAC 1 cut(s) 406
AfiI CCNNNNNNNGG 3 cut(s) 284, 432, 764
AflII CTTAAG 1 cut(s) 526
AhlI ACTAGT 1 cut(s) 329
AjiI CACGTC 1 cut(s) 101
AluBI AGCT 3 cut(s) 143, 473, 1169
AluI AGCT 3 cut(s) 143, 473, 1169
Alw26I GTCTC 2 cut(s) 441, 680
AlwI GGATC 2 cut(s) 417, 430
Ama87I CYCGRG 1 cut(s) 885
AoxI GGCC 3 cut(s) 73, 294, 415
ApeKI GCWGC 2 cut(s) 1098, 1169
ApoI RAATTY 1 cut(s) 822
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AspS9I GGNCC 2 cut(s) 275, 755
AsuC2I CCSGG 3 cut(s) 240, 397, 759
AvaI CYCGRG 1 cut(s) 885
AvaII GGWCC 2 cut(s) 275, 755
AxyI CCTNAGG 1 cut(s) 612
BamHI GGATCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 886
BbsI GAAGAC 1 cut(s) 1062
BbvI GCAGC 2 cut(s) 1110, 1156
BccI CCATC 5 cut(s) 206, 343, 626, 1123, 1190
BceAI ACGGC 1 cut(s) 430
BciVI GTATCC 1 cut(s) 1003
BcnI CCSGG 3 cut(s) 240, 397, 759
BcoDI GTCTC 2 cut(s) 441, 680
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 3 cut(s) 23, 330, 1230
BfmI CTRYAG 1 cut(s) 390
BfrI CTTAAG 1 cut(s) 526
BfuI GTATCC 1 cut(s) 1003
BisI GCNGC 2 cut(s) 1099, 1170
BlsI GCNGC 2 cut(s) 1100, 1171
Bme1390I CCNGG 3 cut(s) 240, 397, 759
Bme18I GGWCC 2 cut(s) 275, 755
BmeT110I CYCGRG 1 cut(s) 885
BmgBI CACGTC 1 cut(s) 101
BmgT120I GGNCC 2 cut(s) 275, 755
BmiI GGNNCC 4 cut(s) 424, 609, 757, 1063
BmrFI CCNGG 3 cut(s) 240, 397, 759
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 4 cut(s) 120, 560, 669, 1212
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 1062
BpuMI CCSGG 3 cut(s) 240, 397, 759
Bsa29I ATCGAT 1 cut(s) 969
BsaI GGTCTC 2 cut(s) 441, 680
BsaJI CCNNGG 4 cut(s) 114, 396, 418, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 432, 764
Bse118I RCCGGY 1 cut(s) 689
Bse1I ACTGG 1 cut(s) 260
Bse21I CCTNAGG 1 cut(s) 612
BseCI ATCGAT 1 cut(s) 969
BseDI CCNNGG 4 cut(s) 114, 396, 418, 426
BseGI GGATG 3 cut(s) 217, 575, 1221
BseLI CCNNNNNNNGG 3 cut(s) 284, 432, 764
BseMII CTCAG 2 cut(s) 285, 603
BseNI ACTGG 1 cut(s) 260
BseXI GCAGC 2 cut(s) 1110, 1156
BshFI GGCC 3 cut(s) 75, 296, 417
BshVI ATCGAT 1 cut(s) 969
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 4 cut(s) 240, 396, 690, 759
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 3 cut(s) 284, 432, 764
BsmAI GTCTC 2 cut(s) 441, 680
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 3 cut(s) 75, 296, 417
Bso31I GGTCTC 2 cut(s) 441, 680
BsoBI CYCGRG 1 cut(s) 885
Bsp1286I GDGCHC 1 cut(s) 886
Bsp143I GATC 4 cut(s) 422, 530, 678, 970
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 3 cut(s) 236, 658, 753
BspANI GGCC 3 cut(s) 75, 296, 417
BspCNI CTCAG 2 cut(s) 284, 604
BspDI ATCGAT 1 cut(s) 969
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 4 cut(s) 424, 609, 757, 1063
BspPI GGATC 2 cut(s) 417, 430
BspQI GCTCTTC 1 cut(s) 211
BspTI CTTAAG 1 cut(s) 526
BspTNI GGTCTC 2 cut(s) 441, 680
BsrFI RCCGGY 1 cut(s) 689
BsrI ACTGG 1 cut(s) 260
BssAI RCCGGY 1 cut(s) 689
BssECI CCNNGG 4 cut(s) 114, 396, 418, 426
BssMI GATC 4 cut(s) 422, 530, 678, 970
BssT1I CCWWGG 2 cut(s) 114, 418
Bst4CI ACNGT 2 cut(s) 391, 620
Bst6I CTCTTC 1 cut(s) 211
BstAFI CTTAAG 1 cut(s) 526
BstAPI GCANNNNNTGC 1 cut(s) 1113
BstC8I GCNNGC 1 cut(s) 1109
BstDEI CTNAG 2 cut(s) 271, 612
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 3 cut(s) 217, 575, 1221
BstKTI GATC 4 cut(s) 425, 533, 681, 973
BstMAI GTCTC 2 cut(s) 441, 680
BstMBI GATC 4 cut(s) 422, 530, 678, 970
BstMWI GCNNNNNNNGC 2 cut(s) 1104, 1113
BstNSI RCATGY 1 cut(s) 204
BstSCI CCNGG 3 cut(s) 238, 395, 757
BstSFI CTRYAG 1 cut(s) 390
BstV1I GCAGC 2 cut(s) 1110, 1156
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 969
Bsu36I CCTNAGG 1 cut(s) 612
BsuI GTATCC 1 cut(s) 1003
BsuRI GGCC 3 cut(s) 75, 296, 417
BsuTUI ATCGAT 1 cut(s) 969
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 2 cut(s) 708, 1050
BtrI CACGTC 1 cut(s) 101
BtsCI GGATG 3 cut(s) 217, 575, 1221
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 1 cut(s) 1089
Cac8I GCNNGC 1 cut(s) 1109
CciI TCATGA 1 cut(s) 951
Cfr10I RCCGGY 1 cut(s) 689
Cfr13I GGNCC 2 cut(s) 275, 755
ClaI ATCGAT 1 cut(s) 969
Csp6I GTAC 1 cut(s) 405
CviQI GTAC 1 cut(s) 405
DdeI CTNAG 2 cut(s) 271, 612
DpnI GATC 4 cut(s) 424, 532, 680, 972
DpnII GATC 4 cut(s) 422, 530, 678, 970
EaeI YGGCCR 1 cut(s) 415
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
EciI GGCGGA 1 cut(s) 225
Eco130I CCWWGG 2 cut(s) 114, 418
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 2 cut(s) 441, 680
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 2 cut(s) 275, 755
Eco81I CCTNAGG 1 cut(s) 612
Eco88I CYCGRG 1 cut(s) 885
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 114, 418
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 2 cut(s) 114, 418
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 1055
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 1099, 1170
FokI GGATG 3 cut(s) 224, 582, 1208
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 2 cut(s) 1099, 1170
FspBI CTAG 3 cut(s) 23, 330, 1230
GluI GCNGC 2 cut(s) 1099, 1170
HaeIII GGCC 3 cut(s) 75, 296, 417
HapII CCGG 4 cut(s) 240, 396, 690, 759
HinfI GANTC 5 cut(s) 232, 870, 955, 1087, 1157
HpaII CCGG 4 cut(s) 240, 396, 690, 759
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 8 cut(s) 33, 352, 372, 590, 665, 987, 1049, 1092
Hpy188III TCNNGA 4 cut(s) 611, 717, 887, 952
Hpy8I GTNNAC 1 cut(s) 110
Hpy99I CGWCG 1 cut(s) 1177
HpyAV CCTTC 3 cut(s) 65, 143, 901
HpyCH4III ACNGT 2 cut(s) 391, 620
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 9 cut(s) 282, 450, 468, 512, 999, 1027, 1098, 1116, 1236
HpyF10VI GCNNNNNNNGC 2 cut(s) 1104, 1113
HpyF3I CTNAG 2 cut(s) 271, 612
HpySE526I ACGT 1 cut(s) 100
Kzo9I GATC 4 cut(s) 422, 530, 678, 970
LguI GCTCTTC 1 cut(s) 211
LmnI GCTCC 3 cut(s) 140, 613, 1166
Lsp1109I GCAGC 2 cut(s) 1110, 1156
LweI GCATC 4 cut(s) 120, 560, 669, 1212
MaeI CTAG 3 cut(s) 23, 330, 1230
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 6 cut(s) 287, 502, 647, 1039, 1084, 1143
MalI GATC 4 cut(s) 424, 532, 680, 972
MboI GATC 4 cut(s) 422, 530, 678, 970
MboII GAAGA 6 cut(s) 9, 168, 228, 589, 941, 1062
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 886
MluCI AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
MlyI GAGTC 1 cut(s) 1081
MnlI CCTC 9 cut(s) 146, 395, 436, 607, 782, 811, 1075, 1158, 1236
MseI TTAA 5 cut(s) 386, 441, 527, 558, 936
MslI CAYNNNNRTG 1 cut(s) 205
MspCI CTTAAG 1 cut(s) 526
MspI CCGG 4 cut(s) 240, 396, 690, 759
MspR9I CCNGG 3 cut(s) 240, 397, 759
MwoI GCNNNNNNNGC 2 cut(s) 1104, 1113
NciI CCSGG 3 cut(s) 240, 397, 759
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 1055
NdeII GATC 4 cut(s) 422, 530, 678, 970
NlaIV GGNNCC 4 cut(s) 424, 609, 757, 1063
NmuCI GTSAC 1 cut(s) 1084
NspI RCATGY 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 885
PagI TCATGA 1 cut(s) 951
PciSI GCTCTTC 1 cut(s) 211
PfeI GAWTC 4 cut(s) 232, 870, 955, 1157
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 2 cut(s) 1100, 1171
PleI GAGTC 1 cut(s) 1081
PpsI GAGTC 1 cut(s) 1081
PspN4I GGNNCC 4 cut(s) 424, 609, 757, 1063
PspPI GGNCC 2 cut(s) 275, 755
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 1 cut(s) 406
RsaNI GTAC 1 cut(s) 405
RseI CAYNNNNRTG 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 5 cut(s) 386, 441, 527, 558, 936
SatI GCNGC 2 cut(s) 1099, 1170
Sau3AI GATC 4 cut(s) 422, 530, 678, 970
Sau96I GGNCC 2 cut(s) 275, 755
SchI GAGTC 1 cut(s) 1081
ScrFI CCNGG 3 cut(s) 240, 397, 759
SduI GDGCHC 1 cut(s) 886
SfaNI GCATC 4 cut(s) 120, 560, 669, 1212
SfcI CTRYAG 1 cut(s) 390
Sfr274I CTCGAG 1 cut(s) 885
SinI GGWCC 2 cut(s) 275, 755
SlaI CTCGAG 1 cut(s) 885
SmiMI CAYNNNNRTG 1 cut(s) 205
SmlI CTYRAG 2 cut(s) 526, 885
SmoI CTYRAG 2 cut(s) 526, 885
SpeI ACTAGT 1 cut(s) 329
Sse9I AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
SsiI CCGC 3 cut(s) 236, 658, 753
SspI AATATT 2 cut(s) 919, 980
SspMI CTAG 3 cut(s) 23, 330, 1230
StyD4I CCNGG 3 cut(s) 238, 395, 757
StyI CCWWGG 2 cut(s) 114, 418
TaaI ACNGT 2 cut(s) 391, 620
TaiI ACGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 495, 886, 969
TasI AATT 7 cut(s) 355, 383, 642, 666, 708, 768, 822
TfiI GAWTC 4 cut(s) 232, 870, 955, 1157
Tru1I TTAA 5 cut(s) 386, 441, 527, 558, 936
Tru9I TTAA 5 cut(s) 386, 441, 527, 558, 936
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 1 cut(s) 1084
TseI GCWGC 2 cut(s) 1098, 1169
Tsp45I GTSAC 1 cut(s) 1084
TspDTI ATGAA 5 cut(s) 509, 554, 942, 968, 1005
TspRI CASTG 1 cut(s) 1089
Vha464I CTTAAG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 275, 755
XapI RAATTY 1 cut(s) 822
XceI RCATGY 1 cut(s) 204
XhoI CTCGAG 1 cut(s) 885
XmiI GTMKAC 1 cut(s) 109
XspI CTAG 3 cut(s) 23, 330, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.