FvH4_3g01620

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004190
Physical Location & Seq
Reverse (-)
850818 .. 852643
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g01620.t1

Sequence Viewer

Length: 1242 bp
ATGACTCTTTCGATATCTTCCCTAGTGTCTAGGTCTTTCTTGATTCTGTTCTTGTTGGCATGGATAGCAAAAGGCCAAGACCTTGATCTCTTTCGTCAAACAAATGGTGTTGGCCAACCCATGGTTTTCGATGTGATGCGTTATGGAGCTGTTGCAGACGGAAGAACAGATAACAGTGAGAAATTTTTGAAAGCATGGAGTGAAGCATGTAATTCCGATGGAAGAGCAACGGTTATGATTCCTGCCGGAACATTCAAGTTATTCCCAGTTGTATTCTCAGGTCCATGCAAGGGTCCTATCGACTTTATAATTAAGGGAACTTTGATGGCTTCTACTGGTTCATCAGCATTGAGTTCTGAAAGTTGGATAAACTTTCGGAACGTCGAACAACTGACAGTAGCCGGTGGAGGTACCCTGGACGGCCAAGGAGCCTCAGTCTGGCGTCTTAACGATTGCACAACTAATCCGGGATGCAAATCTCTCCCAACTTCGATGACATTCGATTCGGTCACAAATGCAAGCGTTCATGACTTGGTATCACTCAACAGCAAAAATACTCATATCAAGGTTCATGGATCCCAAAGGATACGTTTCAGAAACATTAGAATATCAGCTCCAGAGGACAGTCCAAACACCGATGGCATTAAAATCGGTAACTCATACCGCATTGGAATCAGTCGCTCGATCATTCAGACCGGCGATGACTGTGTAGCAATTTTGTCAGGAAGTCGACAAATTCACGTTACTAATGTCTTTTGCGGTCCTGGACATGGAATCAGCATAGGGAGTCTTGGAGGGCATGCTTATCCAGAATATGATGTGGAAGGAATTTATGTGAGAAATAGCGTTTTCAAAGGGTCTGATAATGGTGTTAGAATCAAAACATATTCTAGAGATTCAAAACCCTTGACGGCTTCAAATATTGTGTATGAAGATATTCATATGATTGATGTCAAGAATCCCATTATCATCGATCAACAATATTGTCCAAGCAGTCAATGCAATAAAGAGGGTTCTTCATCAAATGTGCAAATCAGCGATGTTACATTCAGAAATATATGGGGAACCTCGTCTACCAAAGAAGCAGTTACTTTGAAATGCAGCAAAACTAAGCCATGCAAGAATATAAAGATGGAGAATATCAAGTTACCTTATAATGGTCCCAAAGGACCTGCAACTACCGTCTGTTTTAATGTCATCGGCAACTCTATTGGCTCACACATCCCTCCTTCTTGCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

414

Amino Acids

44.61

Weight (kDa)

8.84

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 399 2.8e-97 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 308, 1155, 1240
Acc36I ACCTGC 1 cut(s) 1180
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 121
AccI GTMKAC 2 cut(s) 730, 1073
AciI CCGC 2 cut(s) 664, 759
AclWI GGATC 2 cut(s) 570, 583
AcoI YGGCCR 2 cut(s) 112, 421
AcsI RAATTY 3 cut(s) 182, 735, 828
AcyI GRCGYC 1 cut(s) 442
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 5 cut(s) 121, 290, 438, 770, 1157
AgsI TTSAA 6 cut(s) 190, 256, 853, 900, 918, 1096
AjnI CCWGG 2 cut(s) 414, 763
AluBI AGCT 2 cut(s) 149, 614
AluI AGCT 2 cut(s) 149, 614
AlwI GGATC 2 cut(s) 570, 583
AoxI GGCC 3 cut(s) 73, 112, 421
ApeKI GCWGC 1 cut(s) 1101
ApoI RAATTY 3 cut(s) 182, 735, 828
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
Asp700I GAANNNNTTC 1 cut(s) 936
Asp718I GGTACC 1 cut(s) 410
AspS9I GGNCC 5 cut(s) 281, 293, 761, 1160, 1169
AsuC2I CCSGG 1 cut(s) 468
AvaII GGWCC 5 cut(s) 281, 293, 761, 1160, 1169
BalI TGGCCA 1 cut(s) 114
BamHI GGATCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 1113
BccI CCATC 4 cut(s) 212, 319, 632, 1126
BceAI ACGGC 2 cut(s) 436, 927
BcgI CGANNNNNNTGC 2 cut(s) 631, 665
BciT130I CCWGG 2 cut(s) 416, 765
BciVI GTATCC 1 cut(s) 579
BcnI CCSGG 1 cut(s) 468
BfaI CTAG 3 cut(s) 23, 30, 891
BfuAI ACCTGC 1 cut(s) 1180
BfuI GTATCC 1 cut(s) 579
BisI GCNGC 1 cut(s) 1102
BlsI GCNGC 1 cut(s) 1103
Bme1390I CCNGG 3 cut(s) 416, 468, 765
Bme18I GGWCC 5 cut(s) 281, 293, 761, 1160, 1169
BmgT120I GGNCC 5 cut(s) 281, 293, 761, 1160, 1169
BmiI GGNNCC 6 cut(s) 294, 412, 430, 577, 1066, 1162
BmrFI CCNGG 3 cut(s) 416, 468, 765
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 2 cut(s) 126, 461
BmuI ACTGGG 1 cut(s) 260
BpmI CTGGAG 1 cut(s) 600
BpuMI CCSGG 1 cut(s) 468
Bsa29I ATCGAT 1 cut(s) 972
BsaBI GATNNNNATC 1 cut(s) 475
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 3 cut(s) 120, 414, 424
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 290, 438, 770, 1157
Bse118I RCCGGY 2 cut(s) 401, 695
Bse1I ACTGG 2 cut(s) 266, 340
Bse8I GATNNNNATC 1 cut(s) 475
BseBI CCWGG 2 cut(s) 416, 765
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 3 cut(s) 120, 414, 424
BseGI GGATG 2 cut(s) 476, 1221
BseJI GATNNNNATC 1 cut(s) 475
BseLI CCNNNNNNNGG 5 cut(s) 121, 290, 438, 770, 1157
BseMII CTCAG 2 cut(s) 291, 447
BseNI ACTGG 2 cut(s) 266, 340
BseXI GCAGC 1 cut(s) 1113
BshFI GGCC 3 cut(s) 75, 114, 423
BshNI GGYRCC 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 972
BsiSI CCGG 4 cut(s) 246, 402, 467, 696
BslFI GGGAC 1 cut(s) 1146
BslI CCNNNNNNNGG 5 cut(s) 121, 290, 438, 770, 1157
BsmFI GGGAC 1 cut(s) 1146
BsnI GGCC 3 cut(s) 75, 114, 423
Bsp143I GATC 4 cut(s) 85, 575, 684, 973
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 2 cut(s) 664, 759
BspANI GGCC 3 cut(s) 75, 114, 423
BspCNI CTCAG 2 cut(s) 290, 446
BspDI ATCGAT 1 cut(s) 972
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 6 cut(s) 294, 412, 430, 577, 1066, 1162
BspMI ACCTGC 1 cut(s) 1180
BspPI GGATC 2 cut(s) 570, 583
BspQI GCTCTTC 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 410
BsrFI RCCGGY 2 cut(s) 401, 695
BsrI ACTGG 2 cut(s) 266, 340
BssAI RCCGGY 2 cut(s) 401, 695
BssECI CCNNGG 3 cut(s) 120, 414, 424
BssMI GATC 4 cut(s) 85, 575, 684, 973
BssNI GRCGYC 1 cut(s) 442
BssT1I CCWWGG 2 cut(s) 120, 424
Bst2UI CCWGG 2 cut(s) 416, 765
Bst4CI ACNGT 6 cut(s) 176, 232, 397, 626, 707, 1183
Bst6I CTCTTC 1 cut(s) 217
BstACI GRCGYC 1 cut(s) 442
BstAPI GCANNNNNTGC 1 cut(s) 999
BstC8I GCNNGC 2 cut(s) 520, 801
BstDEI CTNAG 3 cut(s) 277, 433, 1110
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 476, 1221
BstKTI GATC 4 cut(s) 88, 578, 687, 976
BstMBI GATC 4 cut(s) 85, 575, 684, 973
BstMWI GCNNNNNNNGC 2 cut(s) 65, 999
BstNI CCWGG 2 cut(s) 416, 765
BstNSI RCATGY 2 cut(s) 210, 803
BstSCI CCNGG 3 cut(s) 414, 466, 763
BstV1I GCAGC 1 cut(s) 1113
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
Bsu15I ATCGAT 1 cut(s) 972
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 3 cut(s) 75, 114, 423
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 2 cut(s) 714, 1053
BtsCI GGATG 2 cut(s) 476, 1221
BtsIMutI CAGTG 1 cut(s) 181
BveI ACCTGC 1 cut(s) 1180
Cac8I GCNNGC 2 cut(s) 520, 801
CciI TCATGA 1 cut(s) 526
Cfr10I RCCGGY 2 cut(s) 401, 695
Cfr13I GGNCC 5 cut(s) 281, 293, 761, 1160, 1169
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 411
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 3 cut(s) 277, 433, 1110
DpnI GATC 4 cut(s) 87, 577, 686, 975
DpnII GATC 4 cut(s) 85, 575, 684, 973
EaeI YGGCCR 2 cut(s) 112, 421
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 120, 424
Eco32I GATATC 1 cut(s) 15
Eco47I GGWCC 5 cut(s) 281, 293, 761, 1160, 1169
EcoO109I RGGNCCY 2 cut(s) 293, 1169
EcoRII CCWGG 2 cut(s) 414, 763
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 2 cut(s) 120, 424
ErhI CCWWGG 2 cut(s) 120, 424
FaqI GGGAC 1 cut(s) 1146
FauNDI CATATG 1 cut(s) 942
FblI GTMKAC 2 cut(s) 730, 1073
Fnu4HI GCNGC 1 cut(s) 1102
FokI GGATG 2 cut(s) 483, 1208
Fsp4HI GCNGC 1 cut(s) 1102
FspBI CTAG 3 cut(s) 23, 30, 891
GluI GCNGC 1 cut(s) 1102
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 3 cut(s) 75, 114, 423
HapII CCGG 4 cut(s) 246, 402, 467, 696
HgaI GACGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HpaII CCGG 4 cut(s) 246, 402, 467, 696
Hpy166II GTNNAC 2 cut(s) 731, 1074
Hpy188I TCNGA 7 cut(s) 217, 358, 378, 596, 693, 862, 1052
Hpy188III TCNNGA 7 cut(s) 40, 527, 617, 723, 809, 891, 955
Hpy8I GTNNAC 2 cut(s) 731, 1074
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 2 cut(s) 818, 1239
HpyCH4III ACNGT 6 cut(s) 176, 232, 397, 626, 707, 1183
HpyCH4IV ACGT 3 cut(s) 381, 589, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 999
HpyF3I CTNAG 3 cut(s) 277, 433, 1110
HpySE526I ACGT 3 cut(s) 381, 589, 741
Hsp92I GRCGYC 1 cut(s) 442
KpnI GGTACC 1 cut(s) 414
Kzo9I GATC 4 cut(s) 85, 575, 684, 973
LguI GCTCTTC 1 cut(s) 217
LmnI GCTCC 3 cut(s) 146, 428, 619
Lsp1109I GCAGC 1 cut(s) 1113
LweI GCATC 2 cut(s) 126, 461
MaeI CTAG 3 cut(s) 23, 30, 891
MaeII ACGT 3 cut(s) 381, 589, 741
MaeIII GTNAC 6 cut(s) 508, 653, 742, 1042, 1087, 1146
MalI GATC 4 cut(s) 87, 577, 686, 975
MboI GATC 4 cut(s) 85, 575, 684, 973
MboII GAAGA 5 cut(s) 9, 174, 234, 944, 1008
MflI RGATCY 1 cut(s) 575
MlsI TGGCCA 1 cut(s) 114
MluCI AATT 6 cut(s) 182, 211, 309, 714, 735, 828
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 1 cut(s) 796
MmeI TCCRAC 1 cut(s) 344
MnlI CCTC 7 cut(s) 401, 442, 613, 788, 1003, 1078, 1236
Mox20I TGGCCA 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 936
MscI TGGCCA 1 cut(s) 114
MseI TTAA 4 cut(s) 312, 447, 645, 1191
Msp20I TGGCCA 1 cut(s) 114
MspI CCGG 4 cut(s) 246, 402, 467, 696
MspR9I CCNGG 3 cut(s) 416, 468, 765
MvaI CCWGG 2 cut(s) 416, 765
MwoI GCNNNNNNNGC 2 cut(s) 65, 999
NciI CCSGG 1 cut(s) 468
NcoI CCATGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 942
NdeII GATC 4 cut(s) 85, 575, 684, 973
NlaIV GGNNCC 6 cut(s) 294, 412, 430, 577, 1066, 1162
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 2 cut(s) 210, 803
PaeI GCATGC 1 cut(s) 803
PagI TCATGA 1 cut(s) 526
PciSI GCTCTTC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 8 cut(s) 43, 238, 503, 672, 774, 876, 896, 958
PflMI CCANNNNNTGG 1 cut(s) 121
PfoI TCCNGGA 2 cut(s) 466, 763
PkrI GCNGC 1 cut(s) 1103
PleI GAGTC 1 cut(s) 795
PpsI GAGTC 1 cut(s) 795
PpuMI RGGWCCY 2 cut(s) 293, 1169
PsiI TTATAA 3 cut(s) 308, 1155, 1240
Psp5II RGGWCCY 2 cut(s) 293, 1169
Psp6I CCWGG 2 cut(s) 414, 763
PspGI CCWGG 2 cut(s) 414, 763
PspN4I GGNNCC 6 cut(s) 294, 412, 430, 577, 1066, 1162
PspPI GGNCC 5 cut(s) 281, 293, 761, 1160, 1169
PspPPI RGGWCCY 2 cut(s) 293, 1169
PsrI GAACNNNNNNTAC 2 cut(s) 1057, 1089
PsuI RGATCY 1 cut(s) 575
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SalI GTCGAC 1 cut(s) 729
SapI GCTCTTC 1 cut(s) 217
SaqAI TTAA 4 cut(s) 312, 447, 645, 1191
SatI GCNGC 1 cut(s) 1102
Sau3AI GATC 4 cut(s) 85, 575, 684, 973
Sau96I GGNCC 5 cut(s) 281, 293, 761, 1160, 1169
SchI GAGTC 1 cut(s) 796
ScrFI CCNGG 3 cut(s) 416, 468, 765
SfaNI GCATC 2 cut(s) 126, 461
SinI GGWCC 5 cut(s) 281, 293, 761, 1160, 1169
SphI GCATGC 1 cut(s) 803
Sse9I AATT 6 cut(s) 182, 211, 309, 714, 735, 828
SsiI CCGC 2 cut(s) 664, 759
SspI AATATT 2 cut(s) 922, 983
SspMI CTAG 3 cut(s) 23, 30, 891
StyD4I CCNGG 3 cut(s) 414, 466, 763
StyI CCWWGG 2 cut(s) 120, 424
TaaI ACNGT 6 cut(s) 176, 232, 397, 626, 707, 1183
TaiI ACGT 3 cut(s) 384, 592, 744
TaqI TCGA 9 cut(s) 11, 129, 300, 384, 491, 501, 683, 730, 972
TaqII GACCGA 1 cut(s) 496
TasI AATT 6 cut(s) 182, 211, 309, 714, 735, 828
TfiI GAWTC 8 cut(s) 43, 238, 503, 672, 774, 876, 896, 958
Tru1I TTAA 4 cut(s) 312, 447, 645, 1191
Tru9I TTAA 4 cut(s) 312, 447, 645, 1191
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 1 cut(s) 1101
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 6 cut(s) 330, 515, 560, 929, 945, 1008
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 121
VpaK11BI GGWCC 5 cut(s) 281, 293, 761, 1160, 1169
XapI RAATTY 3 cut(s) 182, 735, 828
XbaI TCTAGA 1 cut(s) 890
XceI RCATGY 2 cut(s) 210, 803
XmiI GTMKAC 2 cut(s) 730, 1073
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 3 cut(s) 23, 30, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.