FvH4_3g08040

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001627
Physical Location & Seq
Reverse (-)
4738579 .. 4742405
3827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08040.t2

Sequence Viewer

Length: 1770 bp
ATGGAACCTTGTACAGACATCGGCGCAACGCCTCGGCCCACTTCAGCTCCGCTTCCGGTGGTCCCCGCCGCCTGCAGCGGCACTCTGGGCCGATACCTGGCTCGCCGGCTCGTCGATATCGGCGTTCATGACGTGTTCTCCGTCCCCGGCGACTTCAACTTGACTCTCTTGGACCACCTGATCGCTGAACCCGAGCTCAACCTCATCGGCTGCTGCAACGAGCTCAACGCCGGGTACGCCGCCGACGGCTACGCGCGCGCCAGGGGCGTCGGCGCGTGTGTCGTCACTTTCACCGTTGGTGGGCTCAGCGTCTTGAACGCTATCGCCGGTGCTTGCAGTGAGAATTTGCCGGTGATTTGTATCGTTGGCGGGCCCAACTCCAATGATTACGGGACTAATCGGATCCTCCACCACACCATCGGGTCGCCCGATTTCTCTCAGGAGCTCCGGTGCTTCCAAACTGTGACTTGTTACCAGGCAGTGGTGAATAATTTGGAAGATGGGCATGAGCTTATCGACACGGCAATTTCGACAGCATTGAAGGAGAGCAAGCCGGTGTACATCAGCATAAGTTGTAATTTGCCTGCAATTCCTCACCCCACGTTTGGTAGAGACCCTGTTCCCTTCTTTCTTGAGCCAAAAGTAAGCAATCCGTTAGGGTTGGAGGCAGCTGTTGAAGCAACTGCTGCTTTCCTGAACAAGGCAGTGAAGCCTGTGCTTGTTGGTGGGCCCAAGCTAAGAGTGGCAAAGGCTCAGAAGGCCTTTGTGGAGCTTGCAGATGCTAGTGGGTACCCGTTAGCAGTTATGCCTTCCGGTAAGGGTATGGTGCCGGAGCACCACCCACATTTCATTGGGACTTATTGGGGTGCTGTGAGCACTAGTTTCTGTGGAGAGATTGTGGAGTCTGCAGATGCTTATGTTTTTGCTGGCCCGATTTTTAATGATTATAGCTCTGTTGGGTACTCCTTGCTGATCAAGAAGGAGAAGGCAATTATTGTGGAGCCAAATCGTGTGACCATTGGGAATGGTCCATCATTTTGCTGGGTTTTCATGGGTGACTTCTTGAGTGCATTAGCCAAGAAGCTGAAGAAAAACAGTACCGGTATGGAGAACTACCGCAGAATTTTTGTTCCCCCAGGAATGCCTCTGAAAGCTCAGAAAGATGAACCTCTTAGAGTAAATGTTCTATTTAAGCACATTCAGGATATTGTCACCCCAGAAACTGCCATAATTGCCGAAACTGGTGACTCATGGTTCAATTGTCAGAAGCTCCGCCTCCCTGAGAATTGTGGGTATGAATTCCAGATGCAGTATGGATCTATTGGGTGGTCAGTGGGTGCTACTCTCGGCTATGCTCAGGGTGCCAAAGATAAACGTGTAATTGCTTGCATTGGTGACGGGAGTTTCCAGGTAACTGCCCAGGACATTTCAACAATGATCCGATGTGGACAGAATACCATCATATTTCTTATCAACAACGGAGGCTATACAATCGAAGTAGAGATTCATGATGGACCATATAATGTCATTAAGAACTGGGATTATACTGGTCTTGTTGATGCAATCCACAATGGTGAAGGCAAATGCTGGACTACCAAGGTTCGTACCGAGGAGGACTTAACGGAAGCAATTGCGAAAGCAACAGGGGAGCACAAGGACAGCTTATGCTTCATCGAAGTGTTTGTGCACAAAGATGACACTAGCAAAGAGCTTCTGGAATGGGGATCCCGAGTTTCAGCTGCTAACAGCCGACCTCCAAATCCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

590

Amino Acids

63.62

Weight (kDa)

5.59

Isoelectric Point (pI)

30.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 28 - 137 6.5e-29 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 225 - 342 2.6e-22 Thiamine pyrophosphate enzyme, central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 3 cut(s) 789, 826, 1361
AccB7I CCANNNNNTGG 1 cut(s) 481
AccII CGCG 4 cut(s) 254, 256, 258, 275
AciI CCGC 8 cut(s) 50, 66, 69, 78, 240, 369, 1117, 1273
AclWI GGATC 6 cut(s) 397, 410, 1324, 1432, 1719, 1732
AcsI RAATTY 3 cut(s) 343, 1122, 1298
AcuI CTGAAG 2 cut(s) 27, 1106
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 7 cut(s) 13, 236, 560, 791, 962, 1099, 1606
AfiI CCNNNNNNNGG 5 cut(s) 97, 300, 481, 605, 700
AflIII ACRYGT 2 cut(s) 132, 1375
AgeI ACCGGT 1 cut(s) 1100
AgsI TTSAA 6 cut(s) 157, 316, 541, 677, 1258, 1431
AhlI ACTAGT 1 cut(s) 878
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 6 cut(s) 96, 260, 474, 1135, 1407, 1419
AleI CACNNNNGTG 1 cut(s) 1572
Alw21I GWGCWC 7 cut(s) 198, 225, 447, 837, 878, 1653, 1689
Alw26I GTCTC 1 cut(s) 606
Alw44I GTGCAC 1 cut(s) 1685
AlwI GGATC 6 cut(s) 397, 410, 1324, 1432, 1719, 1732
AlwNI CAGNNNCTG 1 cut(s) 1223
Ama87I CYCGRG 2 cut(s) 191, 1728
AoxI GGCC 6 cut(s) 35, 88, 371, 728, 759, 928
ApaI GGGCCC 2 cut(s) 375, 732
ApaLI GTGCAC 1 cut(s) 1685
ApeKI GCWGC 6 cut(s) 75, 210, 213, 668, 686, 1739
ApoI RAATTY 3 cut(s) 343, 1122, 1298
AsiGI ACCGGT 1 cut(s) 1100
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 5 cut(s) 26, 256, 258, 260, 275
AsuC2I CCSGG 2 cut(s) 147, 232
AsuHPI GGTGA 9 cut(s) 283, 364, 496, 587, 1067, 1204, 1256, 1406, 1586
AvaI CYCGRG 2 cut(s) 191, 1728
AvaII GGWCC 4 cut(s) 61, 172, 1028, 1514
BaeGI GKGCMC 3 cut(s) 375, 732, 1689
BamHI GGATCC 2 cut(s) 402, 1724
BanI GGYRCC 3 cut(s) 789, 826, 1361
BanII GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Bbv12I GWGCWC 7 cut(s) 198, 225, 447, 837, 878, 1653, 1689
BbvI GCAGC 6 cut(s) 87, 197, 200, 673, 680, 1726
BccI CCATC 5 cut(s) 425, 494, 1039, 1466, 1505
BceAI ACGGC 2 cut(s) 262, 537
BciT130I CCWGG 6 cut(s) 98, 262, 476, 1137, 1409, 1421
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 2 cut(s) 147, 232
BcoDI GTCTC 1 cut(s) 606
BcuI ACTAGT 1 cut(s) 878
BfaI CTAG 3 cut(s) 783, 879, 1701
BfmI CTRYAG 2 cut(s) 73, 906
BisI GCNGC 9 cut(s) 69, 76, 79, 211, 214, 240, 669, 687, 1740
BlpI GCTNAGC 1 cut(s) 305
BlsI GCNGC 9 cut(s) 70, 77, 80, 212, 215, 241, 670, 688, 1741
Bme1390I CCNGG 8 cut(s) 98, 147, 232, 262, 476, 1137, 1409, 1421
Bme18I GGWCC 4 cut(s) 61, 172, 1028, 1514
BmeT110I CYCGRG 2 cut(s) 191, 1728
BmgBI CACGTC 1 cut(s) 133
BmrFI CCNGG 8 cut(s) 98, 147, 232, 262, 476, 1137, 1409, 1421
BmrI ACTGGG 1 cut(s) 1546
BmsI GCATC 4 cut(s) 769, 901, 1296, 1549
BmuI ACTGGG 1 cut(s) 1546
Bpu10I CCTNAGC 1 cut(s) 1356
Bpu1102I GCTNAGC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 653, 1084
BpuMI CCSGG 2 cut(s) 147, 232
BsaBI GATNNNNATC 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 606
BsaJI CCNNGG 7 cut(s) 32, 145, 261, 1135, 1419, 1596, 1608
BsaWI WCCGGW 4 cut(s) 55, 447, 812, 1100
BsaXI ACNNNNNCTCC 8 cut(s) 31, 61, 122, 152, 434, 464, 882, 912
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 300, 481, 605, 700
Bse118I RCCGGY 5 cut(s) 105, 326, 349, 553, 1100
Bse1I ACTGG 3 cut(s) 1246, 1541, 1552
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 6 cut(s) 98, 262, 476, 1137, 1409, 1421
BseDI CCNNGG 7 cut(s) 32, 145, 261, 1135, 1419, 1596, 1608
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 5 cut(s) 97, 300, 481, 605, 700
BseMII CTCAG 6 cut(s) 319, 452, 767, 1169, 1272, 1370
BseNI ACTGG 3 cut(s) 1246, 1541, 1552
BsePI GCGCGC 2 cut(s) 254, 256
BseRI GAGGAG 1 cut(s) 1625
BseSI GKGCMC 3 cut(s) 375, 732, 1689
BseXI GCAGC 6 cut(s) 87, 197, 200, 673, 680, 1726
BseYI CCCAGC 1 cut(s) 1041
Bsh1236I CGCG 4 cut(s) 254, 256, 258, 275
BshFI GGCC 6 cut(s) 37, 90, 373, 730, 761, 930
BshNI GGYRCC 3 cut(s) 789, 826, 1361
BshTI ACCGGT 1 cut(s) 1100
BsiHKAI GWGCWC 7 cut(s) 198, 225, 447, 837, 878, 1653, 1689
BsiHKCI CYCGRG 2 cut(s) 191, 1728
BslFI GGGAC 4 cut(s) 47, 128, 406, 868
BslI CCNNNNNNNGG 5 cut(s) 97, 300, 481, 605, 700
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 4 cut(s) 47, 128, 406, 868
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 6 cut(s) 37, 90, 373, 730, 761, 930
Bso31I GGTCTC 1 cut(s) 606
BsoBI CYCGRG 2 cut(s) 191, 1728
Bsp120I GGGCCC 2 cut(s) 371, 728
Bsp1407I TGTACA 2 cut(s) 11, 558
Bsp143I GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 8 cut(s) 50, 66, 69, 78, 240, 369, 1117, 1273
BspANI GGCC 6 cut(s) 37, 90, 373, 730, 761, 930
BspCNI CTCAG 6 cut(s) 318, 451, 766, 1168, 1273, 1369
BspFNI CGCG 4 cut(s) 254, 256, 258, 275
BspHI TCATGA 2 cut(s) 127, 1507
BspMAI CTGCAG 2 cut(s) 77, 910
BspPI GGATC 6 cut(s) 397, 410, 1324, 1432, 1719, 1732
BspT107I GGYRCC 3 cut(s) 789, 826, 1361
BspTNI GGTCTC 1 cut(s) 606
BsrFI RCCGGY 5 cut(s) 105, 326, 349, 553, 1100
BsrGI TGTACA 2 cut(s) 11, 558
BsrI ACTGG 3 cut(s) 1246, 1541, 1552
BssAI RCCGGY 5 cut(s) 105, 326, 349, 553, 1100
BssECI CCNNGG 7 cut(s) 32, 145, 261, 1135, 1419, 1596, 1608
BssHII GCGCGC 2 cut(s) 254, 256
BssMI GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 1596
Bst2UI CCWGG 6 cut(s) 98, 262, 476, 1137, 1409, 1421
Bst4CI ACNGT 3 cut(s) 295, 463, 1097
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 686
BstAUI TGTACA 2 cut(s) 11, 558
BstDEI CTNAG 9 cut(s) 305, 438, 737, 753, 1155, 1172, 1281, 1356, 1764
BstENI CCTNNNNNAGG 1 cut(s) 698
BstFNI CGCG 4 cut(s) 254, 256, 258, 275
BstHHI GCGC 5 cut(s) 26, 256, 258, 260, 275
BstKTI GATC 6 cut(s) 183, 405, 975, 1319, 1440, 1727
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
BstMWI GCNNNNNNNGC 9 cut(s) 87, 236, 255, 264, 677, 686, 758, 1232, 1361
BstNI CCWGG 6 cut(s) 98, 262, 476, 1137, 1409, 1421
BstSCI CCNGG 8 cut(s) 96, 145, 230, 260, 474, 1135, 1407, 1419
BstSFI CTRYAG 2 cut(s) 73, 906
BstSLI GKGCMC 3 cut(s) 375, 732, 1689
BstUI CGCG 4 cut(s) 254, 256, 258, 275
BstV1I GCAGC 6 cut(s) 87, 197, 200, 673, 680, 1726
BstX2I RGATCY 3 cut(s) 402, 1316, 1724
BstYI RGATCY 3 cut(s) 402, 1316, 1724
BsuRI GGCC 6 cut(s) 37, 90, 373, 730, 761, 930
BtrI CACGTC 1 cut(s) 133
BtsI GCAGTG 3 cut(s) 343, 486, 711
BtsIMutI CAGTG 4 cut(s) 343, 486, 711, 1338
CaiI CAGNNNCTG 1 cut(s) 1223
CciI TCATGA 2 cut(s) 127, 1507
CfoI GCGC 5 cut(s) 26, 256, 258, 260, 275
Cfr10I RCCGGY 5 cut(s) 105, 326, 349, 553, 1100
CseI GACGC 2 cut(s) 256, 298
Csp6I GTAC 7 cut(s) 12, 235, 559, 790, 961, 1098, 1605
CspAI ACCGGT 1 cut(s) 1100
CspCI CAANNNNNGTGG 2 cut(s) 978, 1013
CviAII CATG 5 cut(s) 128, 506, 1051, 1251, 1508
CviQI GTAC 7 cut(s) 12, 235, 559, 790, 961, 1098, 1605
DdeI CTNAG 9 cut(s) 305, 438, 737, 753, 1155, 1172, 1281, 1356, 1764
DpnI GATC 6 cut(s) 182, 404, 974, 1318, 1439, 1726
DpnII GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
EciI GGCGGA 1 cut(s) 1262
Ecl136II GAGCTC 3 cut(s) 196, 223, 445
Eco130I CCWWGG 1 cut(s) 1596
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Eco31I GGTCTC 1 cut(s) 606
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 4 cut(s) 61, 172, 1028, 1514
Eco53kI GAGCTC 3 cut(s) 196, 223, 445
Eco57I CTGAAG 2 cut(s) 27, 1106
Eco88I CYCGRG 2 cut(s) 191, 1728
EcoICRI GAGCTC 3 cut(s) 196, 223, 445
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 1298
EcoRII CCWGG 6 cut(s) 96, 260, 474, 1135, 1407, 1419
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 1596
EcoT38I GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
ErhI CCWWGG 1 cut(s) 1596
FaeI CATG 5 cut(s) 131, 509, 1054, 1254, 1511
FalI AAGNNNNNCTT 2 cut(s) 1646, 1678
FaqI GGGAC 4 cut(s) 47, 128, 406, 868
FatI CATG 5 cut(s) 127, 505, 1050, 1250, 1507
FauI CCCGC 2 cut(s) 73, 362
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 9 cut(s) 69, 76, 79, 211, 214, 240, 669, 687, 1740
FriOI GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Fsp4HI GCNGC 9 cut(s) 69, 76, 79, 211, 214, 240, 669, 687, 1740
FspBI CTAG 3 cut(s) 783, 879, 1701
GlaI GCGC 5 cut(s) 25, 255, 257, 259, 274
GluI GCNGC 9 cut(s) 69, 76, 79, 211, 214, 240, 669, 687, 1740
GsaI CCCAGC 1 cut(s) 1045
HaeIII GGCC 6 cut(s) 37, 90, 373, 730, 761, 930
HgaI GACGC 2 cut(s) 256, 298
HhaI GCGC 5 cut(s) 26, 256, 258, 260, 275
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 131, 509, 1054, 1254, 1511
Hin6I GCGC 5 cut(s) 24, 254, 256, 258, 273
HinP1I GCGC 5 cut(s) 24, 254, 256, 258, 273
HinfI GANTC 4 cut(s) 163, 902, 1247, 1504
HphI GGTGA 9 cut(s) 283, 364, 496, 587, 1067, 1204, 1256, 1406, 1586
Hpy166II GTNNAC 3 cut(s) 559, 1448, 1687
Hpy188I TCNGA 6 cut(s) 402, 756, 1149, 1158, 1266, 1442
Hpy8I GTNNAC 3 cut(s) 559, 1448, 1687
Hpy99I CGWCG 3 cut(s) 116, 248, 272
HpyAV CCTTC 7 cut(s) 535, 634, 751, 819, 973, 979, 1571
HpyCH4III ACNGT 3 cut(s) 295, 463, 1097
HpyCH4IV ACGT 3 cut(s) 132, 602, 1375
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 236, 255, 264, 677, 686, 758, 1232, 1361
HpyF3I CTNAG 9 cut(s) 305, 438, 737, 753, 1155, 1172, 1281, 1356, 1764
HpySE526I ACGT 3 cut(s) 132, 602, 1375
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 131, 509, 1054, 1254, 1511
HspAI GCGC 5 cut(s) 24, 254, 256, 258, 273
KpnI GGTACC 1 cut(s) 793
KroI GCCGGC 1 cut(s) 105
KroNI GCCGGC 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
LmnI GCTCC 8 cut(s) 52, 442, 450, 769, 832, 1000, 1275, 1648
Lsp1109I GCAGC 6 cut(s) 87, 197, 200, 673, 680, 1726
LweI GCATC 4 cut(s) 769, 901, 1296, 1549
MaeI CTAG 3 cut(s) 783, 879, 1701
MaeII ACGT 3 cut(s) 132, 602, 1375
MaeIII GTNAC 9 cut(s) 283, 463, 470, 1012, 1055, 1210, 1244, 1394, 1411
MalI GATC 6 cut(s) 182, 404, 974, 1318, 1439, 1726
MauBI CGCGCGCG 1 cut(s) 254
MboI GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
MboII GAAGA 2 cut(s) 509, 1099
MfeI CAATTG 2 cut(s) 1258, 1629
MflI RGATCY 3 cut(s) 402, 1316, 1724
MlyI GAGTC 3 cut(s) 157, 911, 1241
MmeI TCCRAC 1 cut(s) 642
MroNI GCCGGC 1 cut(s) 105
MseI TTAA 4 cut(s) 939, 1191, 1530, 1619
MslI CAYNNNNRTG 3 cut(s) 1572, 1676, 1692
MspA1I CMGCKG 3 cut(s) 78, 671, 1739
MspR9I CCNGG 8 cut(s) 98, 147, 232, 262, 476, 1137, 1409, 1421
MunI CAATTG 2 cut(s) 1258, 1629
Mva1269I GAATGC 1 cut(s) 1146
MvaI CCWGG 6 cut(s) 98, 262, 476, 1137, 1409, 1421
MvnI CGCG 4 cut(s) 254, 256, 258, 275
MwoI GCNNNNNNNGC 9 cut(s) 87, 236, 255, 264, 677, 686, 758, 1232, 1361
NaeI GCCGGC 1 cut(s) 107
NciI CCSGG 2 cut(s) 147, 232
NdeII GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
NgoMIV GCCGGC 1 cut(s) 105
NlaIII CATG 5 cut(s) 131, 509, 1054, 1254, 1511
NmeAIII GCCGAG 2 cut(s) 13, 1326
NmuCI GTSAC 7 cut(s) 283, 463, 1012, 1055, 1210, 1244, 1394
OliI CACNNNNGTG 1 cut(s) 1572
PagI TCATGA 2 cut(s) 127, 1507
PauI GCGCGC 2 cut(s) 254, 256
PceI AGGCCT 1 cut(s) 761
PcsI WCGNNNNNNNCGW 5 cut(s) 120, 138, 189, 426, 527
PctI GAATGC 1 cut(s) 1146
PdiI GCCGGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 1504
PflMI CCANNNNNTGG 1 cut(s) 481
PinAI ACCGGT 1 cut(s) 1100
PkrI GCNGC 9 cut(s) 70, 77, 80, 212, 215, 241, 670, 688, 1741
PleI GAGTC 3 cut(s) 157, 910, 1241
PpsI GAGTC 3 cut(s) 157, 910, 1241
Psp124BI GAGCTC 3 cut(s) 198, 225, 447
Psp6I CCWGG 6 cut(s) 96, 260, 474, 1135, 1407, 1419
PspFI CCCAGC 1 cut(s) 1041
PspGI CCWGG 6 cut(s) 96, 260, 474, 1135, 1407, 1419
PspOMI GGGCCC 2 cut(s) 371, 728
PstI CTGCAG 2 cut(s) 77, 910
PstNI CAGNNNCTG 1 cut(s) 1223
PsuI RGATCY 3 cut(s) 402, 1316, 1724
PteI GCGCGC 2 cut(s) 254, 256
PvuII CAGCTG 2 cut(s) 671, 1739
RsaI GTAC 7 cut(s) 13, 236, 560, 791, 962, 1099, 1606
RsaNI GTAC 7 cut(s) 12, 235, 559, 790, 961, 1098, 1605
RseI CAYNNNNRTG 3 cut(s) 1572, 1676, 1692
SacI GAGCTC 3 cut(s) 198, 225, 447
SaqAI TTAA 4 cut(s) 939, 1191, 1530, 1619
SatI GCNGC 9 cut(s) 69, 76, 79, 211, 214, 240, 669, 687, 1740
Sau3AI GATC 6 cut(s) 180, 402, 972, 1316, 1437, 1724
SchI GAGTC 3 cut(s) 157, 911, 1241
ScrFI CCNGG 8 cut(s) 98, 147, 232, 262, 476, 1137, 1409, 1421
SfaNI GCATC 4 cut(s) 769, 901, 1296, 1549
SfcI CTRYAG 2 cut(s) 73, 906
SgrAI CRCCGGYG 1 cut(s) 326
SinI GGWCC 4 cut(s) 61, 172, 1028, 1514
SmiMI CAYNNNNRTG 3 cut(s) 1572, 1676, 1692
SmlI CTYRAG 2 cut(s) 632, 1063
SmoI CTYRAG 2 cut(s) 632, 1063
SpeI ACTAGT 1 cut(s) 878
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 8 cut(s) 50, 66, 69, 78, 240, 369, 1117, 1273
SspMI CTAG 3 cut(s) 783, 879, 1701
SstI GAGCTC 3 cut(s) 198, 225, 447
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 8 cut(s) 96, 145, 230, 260, 474, 1135, 1407, 1419
StyI CCWWGG 1 cut(s) 1596
TaaI ACNGT 3 cut(s) 295, 463, 1097
TaiI ACGT 3 cut(s) 135, 605, 1378
TaqI TCGA 5 cut(s) 114, 516, 530, 1494, 1674
TatI WGTACW 2 cut(s) 11, 558
TauI GCSGC 3 cut(s) 71, 81, 242
TfiI GAWTC 1 cut(s) 1504
Tru1I TTAA 4 cut(s) 939, 1191, 1530, 1619
Tru9I TTAA 4 cut(s) 939, 1191, 1530, 1619
TscAI CASTG 4 cut(s) 343, 486, 711, 1338
TseFI GTSAC 7 cut(s) 283, 463, 1012, 1055, 1210, 1244, 1394
TseI GCWGC 6 cut(s) 75, 210, 213, 668, 686, 1739
Tsp45I GTSAC 7 cut(s) 283, 463, 1012, 1055, 1210, 1244, 1394
TspDTI ATGAA 7 cut(s) 116, 838, 1039, 1179, 1311, 1496, 1660
TspGWI ACGGA 4 cut(s) 130, 642, 1494, 1637
TspRI CASTG 4 cut(s) 343, 486, 711, 1338
Van91I CCANNNNNTGG 1 cut(s) 481
VneI GTGCAC 1 cut(s) 1685
VpaK11BI GGWCC 4 cut(s) 61, 172, 1028, 1514
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 3 cut(s) 343, 1122, 1298
XcmI CCANNNNNNNNNTGG 3 cut(s) 739, 1038, 1310
XspI CTAG 3 cut(s) 783, 879, 1701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.