Rw5G042960

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001627
Physical Location & Seq
Forward (+)
75095972 .. 75098893
2922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042960.1

Sequence Viewer

Length: 1815 bp
ATGGCGACTGGCCTCGGCTCCCTAGAGTTTACCAGGCCCACCAACAACAACGTGGGCTGCCCTCCTCAAAACGGCGCCGCTGCACTCCACGACCACCCTCCCTCCACTATCTCCTCCCCTGACTCCACCCTCGGCCGCCACCTGGCACACCGCCTCGTGGAGGTGGGTGTCACTGACGTGTTCTCTGTCCCTGGTGACTTTAACCTAACCCTTCTTGACCACCTCATTGCCGAGCCGGGGCTCACCAACATTGGTTGCTGCAATGAGCTCAATGCCGGCTACGCCGCGGATGGGTACGCCCGGTCTCGCGGGGTAGGGGCTTGTGTGGTCACGTTTACTGTGGGTGGGCTTAGTGTGCTTAATGCTATTGCTGGGGCATATAGTGAGAATCTACCTGTGATATGTGTTGTTGGAGGACCTAACACAAATGATTATGGCACCAACAGAATTCTTCACCACACTATCGGCTTGGCGGATTTTAGCCAAGAATTCAGGTGCTTCCAGTCTGTCACATGCTATCAAGCTGTGGTGAACAACTTGGAAGATGCACATGAGCAGATTGATACAGCCATTTCTACTGCTCTCAAGCAAAGCAAGCCTGTCTATATTAGCATTAGTTGTAACTTGCCTGGGATTTCACATCCAACTTTTGGCAGAGAGCCTATTCCATTTTCATTGTCACCTAGAATGAGTAACCAGAGGGGGCTAGAGGCAGCTGTGGATGCAGCAGCAGCTTTCCTAAACAAGGCGGTGAAGCCGGTCATGGTTGGAGGACCAAAACTCCGAGTTGCAAAAGCCCATGAAGCGTTTATCGAATTGGCTGATGCCAGTGGCTATGCCCTGGCTGTGATGCCATCAGCCAAAGGCCATGTGGTGGAAACCCATCCTCACTTCATCGGGACATATTGGGGGGCAGTGGGCACTGCCTACTGTGGTGAGATTGTGGAGTCAGCTGATGCGTACGTATTTGTTGGCCCAATTTTCAATGACTACAGCTCTGTTGGGTACTCACTCCTTCTTAAGAAGGAGAAGGCCATCATTGTGCAGCCTGATCGAGTGGTTATAGCCAATGGCCCTTCTTTCGGGTGTGTTCTAATGAAGGACTTTCTCCAGGCACTTGCTAAGAAGATCACACGCAACACTACTGCTTATGAGAATTACCACAGAATCTTTGTCCCGGAAGGCCAGCCTGTCAAATCTGAGCCTAATGAGCCTTTGAGAGTGAATGTTCTGTTCCAACATATTCAGAAAATGCTTTCAGCTGATACAGCTGTTATTGCTGAGACTGGTGATTCTTGGTTTAATTGCCAGAAACTGAAGTTGCCAGAAGGATGTGGGTACGAATTCCAGATGCAATATGGTTCGATTGGGTGGTCTGTTGGTGGAACTCTTGGATATGCACAGGCGGTACCGAAGAAGCGTGTCATTGCTTGCATTGGTGATGGTAGCTTTCAGGTGACCGCACAAGATGTGTCAACAATGATCAGATGTAATCAAAAGAGCATCATATTCCTCATAAACAATGGTGGATACACAATAGAAGTCGAAATCCATGACGGGCCTTACAATGTGATCAAGAACTGGAACTACACTGGTTTAGTGGATGCAATCCACAATGGTGAAGGAAATTGCTGGACAACCAAGGTACGCACTGAAGAGGACCTTACAGAAGCCATTGCAACAGCAACAGGGGACAAGAAAGACTGCTTGTGCTTCATAGAAGTCATTGTTCACAGGGATGACACAAGCAAAGAGCTACTTGAATGGGGTTCCAGGGTCTCTTCCGCCAATAGCCGCCCACCAAATCCGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

604

Amino Acids

65.1

Weight (kDa)

5.77

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 43 - 148 1.2e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 240 - 357 3.3e-22 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 450 - 575 3.8e-14 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1408
AccB1I GGYRCC 3 cut(s) 74, 437, 1408
AccB7I CCANNNNNTGG 2 cut(s) 650, 874
AccII CGCG 2 cut(s) 287, 309
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 3 cut(s) 447, 488, 1343
AcuI CTGAAG 2 cut(s) 1337, 1674
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 6 cut(s) 296, 962, 1007, 1340, 1410, 1647
AflII CTTAAG 1 cut(s) 1019
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 2 cut(s) 985, 1763
AjiI CACGTC 1 cut(s) 178
AjnI CCWGG 7 cut(s) 32, 141, 190, 628, 840, 1110, 1772
AleI CACNNNNGTG 2 cut(s) 176, 1617
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 3 cut(s) 309, 1277, 1783
AlwNI CAGNNNCTG 1 cut(s) 1315
AoxI GGCC 9 cut(s) 10, 35, 133, 865, 973, 1032, 1072, 1183, 1559
ApeKI GCWGC 8 cut(s) 57, 80, 258, 713, 725, 728, 731, 1045
ApoI RAATTY 3 cut(s) 447, 488, 1343
Asp718I GGTACC 1 cut(s) 1408
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 7 cut(s) 36, 416, 773, 974, 1073, 1559, 1660
AsuC2I CCSGG 3 cut(s) 237, 301, 1178
AvaII GGWCC 3 cut(s) 416, 773, 1660
BaeGI GKGCMC 1 cut(s) 923
BaeI ACNNNNGTAYC 2 cut(s) 1257, 1290
BanI GGYRCC 3 cut(s) 74, 437, 1408
BanII GRGCYC 2 cut(s) 243, 270
BauI CACGAG 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 8 cut(s) 44, 67, 245, 725, 737, 740, 743, 1057
BccI CCATC 5 cut(s) 284, 862, 891, 1043, 1436
BceAI ACGGC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 7 cut(s) 34, 143, 192, 630, 842, 1112, 1774
BciVI GTATCC 1 cut(s) 1523
BclI TGATCA 2 cut(s) 1482, 1572
BcnI CCSGG 3 cut(s) 237, 301, 1178
BcoDI GTCTC 3 cut(s) 309, 1277, 1783
BfaI CTAG 3 cut(s) 23, 684, 707
BfmI CTRYAG 1 cut(s) 991
BfoI RGCGCY 1 cut(s) 78
BfrI CTTAAG 1 cut(s) 1019
BfuI GTATCC 1 cut(s) 1523
Bme18I GGWCC 3 cut(s) 416, 773, 1660
BmgBI CACGTC 1 cut(s) 178
BmgT120I GGNCC 7 cut(s) 36, 416, 773, 974, 1073, 1559, 1660
BmiI GGNNCC 5 cut(s) 19, 76, 439, 1410, 1771
BmsI GCATC 8 cut(s) 535, 712, 814, 840, 946, 1341, 1512, 1594
BpmI CTGGAG 1 cut(s) 1094
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 3 cut(s) 237, 301, 1178
BsaAI YACGTR 1 cut(s) 964
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 2 cut(s) 309, 1783
BsaJI CCNNGG 9 cut(s) 13, 130, 190, 236, 285, 629, 840, 1641, 1773
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 765, 795
Bse118I RCCGGY 2 cut(s) 275, 757
Bse1I ACTGG 6 cut(s) 13, 502, 828, 1291, 1586, 1597
Bse3DI GCAATG 4 cut(s) 225, 268, 1425, 1674
BseBI CCWGG 7 cut(s) 34, 143, 192, 630, 842, 1112, 1774
BseDI CCNNGG 9 cut(s) 13, 130, 190, 236, 285, 629, 840, 1641, 1773
BseGI GGATG 7 cut(s) 295, 640, 727, 883, 1337, 1609, 1744
BseMI GCAATG 4 cut(s) 225, 268, 1425, 1674
BseMII CTCAG 2 cut(s) 1191, 1272
BseNI ACTGG 6 cut(s) 13, 502, 828, 1291, 1586, 1597
BseRI GAGGAG 2 cut(s) 54, 103
BseSI GKGCMC 1 cut(s) 923
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 8 cut(s) 44, 67, 245, 725, 737, 740, 743, 1057
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 2 cut(s) 66, 1064
Bsh1236I CGCG 2 cut(s) 287, 309
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 9 cut(s) 12, 37, 135, 867, 975, 1034, 1074, 1185, 1561
BshNI GGYRCC 3 cut(s) 74, 437, 1408
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 5 cut(s) 236, 276, 301, 758, 1178
BsiWI CGTACG 1 cut(s) 960
BslFI GGGAC 4 cut(s) 173, 913, 1160, 1706
BsmAI GTCTC 3 cut(s) 309, 1277, 1783
BsmFI GGGAC 4 cut(s) 173, 913, 1160, 1706
BsnI GGCC 9 cut(s) 12, 37, 135, 867, 975, 1034, 1074, 1185, 1561
Bso31I GGTCTC 2 cut(s) 309, 1783
Bsp1286I GDGCHC 3 cut(s) 243, 270, 923
Bsp143I GATC 4 cut(s) 1051, 1128, 1482, 1572
BspANI GGCC 9 cut(s) 12, 37, 135, 867, 975, 1034, 1074, 1185, 1561
BspCNI CTCAG 2 cut(s) 1192, 1273
BspFNI CGCG 2 cut(s) 287, 309
BspLI GGNNCC 5 cut(s) 19, 76, 439, 1410, 1771
BspT107I GGYRCC 3 cut(s) 74, 437, 1408
BspTI CTTAAG 1 cut(s) 1019
BspTNI GGTCTC 2 cut(s) 309, 1783
BsrDI GCAATG 4 cut(s) 225, 268, 1425, 1674
BsrFI RCCGGY 2 cut(s) 275, 757
BsrI ACTGG 6 cut(s) 13, 502, 828, 1291, 1586, 1597
BssAI RCCGGY 2 cut(s) 275, 757
BssECI CCNNGG 9 cut(s) 13, 130, 190, 236, 285, 629, 840, 1641, 1773
BssMI GATC 4 cut(s) 1051, 1128, 1482, 1572
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 1641
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 7 cut(s) 34, 143, 192, 630, 842, 1112, 1774
Bst4CI ACNGT 2 cut(s) 340, 932
Bst6I CTCTTC 2 cut(s) 1650, 1786
BstACI GRCGYC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 1019
BstBAI YACGTR 1 cut(s) 964
BstC8I GCNNGC 4 cut(s) 277, 596, 1187, 1432
BstDEI CTNAG 4 cut(s) 350, 1122, 1200, 1281
BstDSI CCRYGG 1 cut(s) 285
BstEII GGTNACC 1 cut(s) 1456
BstENI CCTNNNNNAGG 2 cut(s) 158, 743
BstF5I GGATG 7 cut(s) 295, 640, 727, 883, 1337, 1609, 1744
BstFNI CGCG 2 cut(s) 287, 309
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 4 cut(s) 1054, 1131, 1485, 1575
BstMAI GTCTC 3 cut(s) 309, 1277, 1783
BstMBI GATC 4 cut(s) 1051, 1128, 1482, 1572
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 9 cut(s) 281, 355, 595, 722, 731, 803, 1210, 1268, 1277
BstNI CCWGG 7 cut(s) 34, 143, 192, 630, 842, 1112, 1774
BstNSI RCATGY 1 cut(s) 516
BstPI GGTNACC 1 cut(s) 1456
BstSFI CTRYAG 1 cut(s) 991
BstSLI GKGCMC 1 cut(s) 923
BstSNI TACGTA 1 cut(s) 964
BstUI CGCG 2 cut(s) 287, 309
BstV1I GCAGC 8 cut(s) 44, 67, 245, 725, 737, 740, 743, 1057
BstZI CGGCCG 1 cut(s) 133
BsuI GTATCC 1 cut(s) 1523
BsuRI GGCC 9 cut(s) 12, 37, 135, 867, 975, 1034, 1074, 1185, 1561
BtgI CCRYGG 1 cut(s) 285
BtrI CACGTC 1 cut(s) 178
BtsCI GGATG 7 cut(s) 295, 640, 727, 883, 1337, 1609, 1744
BtsI GCAGTG 2 cut(s) 921, 921
BtsIMutI CAGTG 6 cut(s) 171, 835, 921, 921, 1590, 1650
Cac8I GCNNGC 4 cut(s) 277, 596, 1187, 1432
CaiI CAGNNNCTG 1 cut(s) 1315
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 2 cut(s) 275, 757
Cfr13I GGNCC 7 cut(s) 36, 416, 773, 974, 1073, 1559, 1660
Cfr42I CCGCGG 1 cut(s) 288
Csp6I GTAC 6 cut(s) 295, 961, 1006, 1339, 1409, 1646
CviAII CATG 6 cut(s) 513, 551, 763, 800, 869, 1553
CviQI GTAC 6 cut(s) 295, 961, 1006, 1339, 1409, 1646
DdeI CTNAG 4 cut(s) 350, 1122, 1200, 1281
DinI GGCGCC 1 cut(s) 76
DpnI GATC 4 cut(s) 1053, 1130, 1484, 1574
DpnII GATC 4 cut(s) 1051, 1128, 1482, 1572
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 2 cut(s) 1650, 1786
EarI CTCTTC 2 cut(s) 1650, 1786
EciI GGCGGA 2 cut(s) 488, 1774
Ecl136II GAGCTC 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 964
Eco130I CCWWGG 1 cut(s) 1641
Eco24I GRGCYC 2 cut(s) 243, 270
Eco31I GGTCTC 2 cut(s) 309, 1783
Eco47I GGWCC 3 cut(s) 416, 773, 1660
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 1337, 1674
Eco91I GGTNACC 1 cut(s) 1456
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 2 cut(s) 158, 743
EcoO109I RGGNCCY 2 cut(s) 416, 1660
EcoO65I GGTNACC 1 cut(s) 1456
EcoRI GAATTC 3 cut(s) 447, 488, 1343
EcoRII CCWGG 7 cut(s) 32, 141, 190, 628, 840, 1110, 1772
EcoT14I CCWWGG 1 cut(s) 1641
EcoT38I GRGCYC 2 cut(s) 243, 270
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 1641
FaeI CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
FalI AAGNNNNNCTT 4 cut(s) 1647, 1679, 1743, 1775
FaqI GGGAC 4 cut(s) 173, 913, 1160, 1706
FatI CATG 6 cut(s) 512, 550, 762, 799, 868, 1552
FauI CCCGC 1 cut(s) 302
FbaI TGATCA 2 cut(s) 1482, 1572
FokI GGATG 7 cut(s) 302, 627, 734, 870, 1344, 1616, 1751
FriOI GRGCYC 2 cut(s) 243, 270
FspBI CTAG 3 cut(s) 23, 684, 707
GlaI GCGC 1 cut(s) 76
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 1094
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 9 cut(s) 12, 37, 135, 867, 975, 1034, 1074, 1185, 1561
HapII CCGG 5 cut(s) 236, 276, 301, 758, 1178
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 1 cut(s) 1476
HindII GTYRAC 1 cut(s) 1476
HinfI GANTC 5 cut(s) 122, 388, 947, 1167, 1292
HpaII CCGG 5 cut(s) 236, 276, 301, 758, 1178
Hpy166II GTNNAC 5 cut(s) 30, 336, 532, 1476, 1732
Hpy188I TCNGA 4 cut(s) 785, 1201, 1248, 1487
Hpy188III TCNNGA 4 cut(s) 215, 898, 1348, 1576
Hpy8I GTNNAC 5 cut(s) 30, 336, 532, 1476, 1732
HpyAV CCTTC 9 cut(s) 221, 1018, 1024, 1025, 1086, 1093, 1175, 1322, 1616
HpyCH4III ACNGT 2 cut(s) 340, 932
HpyCH4IV ACGT 4 cut(s) 51, 177, 332, 963
HpyF10VI GCNNNNNNNGC 9 cut(s) 281, 355, 595, 722, 731, 803, 1210, 1268, 1277
HpyF3I CTNAG 4 cut(s) 350, 1122, 1200, 1281
HpySE526I ACGT 4 cut(s) 51, 177, 332, 963
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
HspAI GCGC 1 cut(s) 75
KasI GGCGCC 1 cut(s) 74
KpnI GGTACC 1 cut(s) 1412
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
Ksp22I TGATCA 2 cut(s) 1482, 1572
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 4 cut(s) 1051, 1128, 1482, 1572
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 8 cut(s) 44, 67, 245, 725, 737, 740, 743, 1057
LweI GCATC 8 cut(s) 535, 712, 814, 840, 946, 1341, 1512, 1594
MaeI CTAG 3 cut(s) 23, 684, 707
MaeII ACGT 4 cut(s) 51, 177, 332, 963
MaeIII GTNAC 8 cut(s) 169, 194, 328, 508, 620, 678, 692, 1456
MalI GATC 4 cut(s) 1053, 1130, 1484, 1574
MboI GATC 4 cut(s) 1051, 1128, 1482, 1572
MboII GAAGA 6 cut(s) 443, 554, 1138, 1426, 1667, 1773
MhlI GDGCHC 3 cut(s) 243, 270, 923
MluCI AATT 8 cut(s) 447, 488, 815, 978, 1156, 1303, 1343, 1627
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 2 cut(s) 116, 956
MmeI TCCRAC 4 cut(s) 391, 668, 748, 1261
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 4 cut(s) 201, 360, 1020, 1302
MslI CAYNNNNRTG 4 cut(s) 176, 1040, 1617, 1737
MspA1I CMGCKG 6 cut(s) 80, 287, 716, 953, 1262, 1271
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 5 cut(s) 236, 276, 301, 758, 1178
MvaI CCWGG 7 cut(s) 34, 143, 192, 630, 842, 1112, 1774
MvnI CGCG 2 cut(s) 287, 309
MwoI GCNNNNNNNGC 9 cut(s) 281, 355, 595, 722, 731, 803, 1210, 1268, 1277
NaeI GCCGGC 1 cut(s) 277
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 3 cut(s) 237, 301, 1178
NdeII GATC 4 cut(s) 1051, 1128, 1482, 1572
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
NlaIV GGNNCC 5 cut(s) 19, 76, 439, 1410, 1771
NmeAIII GCCGAG 2 cut(s) 111, 256
NmuCI GTSAC 6 cut(s) 169, 194, 328, 508, 678, 1456
NspI RCATGY 1 cut(s) 516
OliI CACNNNNGTG 2 cut(s) 176, 1617
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 3 cut(s) 388, 1167, 1292
Pfl23II CGTACG 1 cut(s) 960
PflMI CCANNNNNTGG 2 cut(s) 650, 874
PfoI TCCNGGA 1 cut(s) 1176
PleI GAGTC 2 cut(s) 116, 955
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 2 cut(s) 116, 955
Ppu21I YACGTR 1 cut(s) 964
PpuMI RGGWCCY 2 cut(s) 416, 1660
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 2 cut(s) 416, 1660
Psp6I CCWGG 7 cut(s) 32, 141, 190, 628, 840, 1110, 1772
PspEI GGTNACC 1 cut(s) 1456
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 7 cut(s) 32, 141, 190, 628, 840, 1110, 1772
PspLI CGTACG 1 cut(s) 960
PspN4I GGNNCC 5 cut(s) 19, 76, 439, 1410, 1771
PspPI GGNCC 7 cut(s) 36, 416, 773, 974, 1073, 1559, 1660
PspPPI RGGWCCY 2 cut(s) 416, 1660
PsrI GAACNNNNNNTAC 2 cut(s) 1571, 1603
PstNI CAGNNNCTG 1 cut(s) 1315
PvuII CAGCTG 4 cut(s) 716, 953, 1262, 1271
RsaI GTAC 6 cut(s) 296, 962, 1007, 1340, 1410, 1647
RsaNI GTAC 6 cut(s) 295, 961, 1006, 1339, 1409, 1646
RseI CAYNNNNRTG 4 cut(s) 176, 1040, 1617, 1737
SacI GAGCTC 1 cut(s) 270
SacII CCGCGG 1 cut(s) 288
SaqAI TTAA 4 cut(s) 201, 360, 1020, 1302
Sau3AI GATC 4 cut(s) 1051, 1128, 1482, 1572
Sau96I GGNCC 7 cut(s) 36, 416, 773, 974, 1073, 1559, 1660
SchI GAGTC 2 cut(s) 116, 956
SduI GDGCHC 3 cut(s) 243, 270, 923
SfaNI GCATC 8 cut(s) 535, 712, 814, 840, 946, 1341, 1512, 1594
SfcI CTRYAG 1 cut(s) 991
SfoI GGCGCC 1 cut(s) 76
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 3 cut(s) 416, 773, 1660
SmiMI CAYNNNNRTG 4 cut(s) 176, 1040, 1617, 1737
SmlI CTYRAG 2 cut(s) 584, 1019
SmoI CTYRAG 2 cut(s) 584, 1019
SnaBI TACGTA 1 cut(s) 964
Sse9I AATT 8 cut(s) 447, 488, 815, 978, 1156, 1303, 1343, 1627
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 3 cut(s) 23, 684, 707
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 1641
TaaI ACNGT 2 cut(s) 340, 932
TaiI ACGT 4 cut(s) 54, 180, 335, 966
TaqI TCGA 4 cut(s) 813, 1054, 1364, 1545
TasI AATT 8 cut(s) 447, 488, 815, 978, 1156, 1303, 1343, 1627
TauI GCSGC 4 cut(s) 80, 138, 287, 1797
TfiI GAWTC 3 cut(s) 388, 1167, 1292
Tru1I TTAA 4 cut(s) 201, 360, 1020, 1302
Tru9I TTAA 4 cut(s) 201, 360, 1020, 1302
TscAI CASTG 6 cut(s) 178, 835, 921, 928, 1597, 1657
TseFI GTSAC 6 cut(s) 169, 194, 328, 508, 678, 1456
TseI GCWGC 8 cut(s) 57, 80, 258, 713, 725, 728, 731, 1045
Tsp45I GTSAC 6 cut(s) 169, 194, 328, 508, 678, 1456
TspDTI ATGAA 5 cut(s) 663, 816, 883, 1112, 1705
TspRI CASTG 6 cut(s) 178, 835, 921, 928, 1597, 1657
Van91I CCANNNNNTGG 2 cut(s) 650, 874
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 3 cut(s) 416, 773, 1660
XagI CCTNNNNNAGG 2 cut(s) 158, 743
XapI RAATTY 3 cut(s) 447, 488, 1343
XceI RCATGY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 3 cut(s) 49, 868, 1355
XspI CTAG 3 cut(s) 23, 684, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.