pycom03g05210

Pyruvate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001627
Physical Location & Seq
Reverse (-)
4042654 .. 4044571
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05210.1

Sequence Viewer

Length: 1224 bp
ATGGCCACCAGCATTGGCTCCCTAGACTCTTCAAAACCTGCCAACAACTGCGTCGGTTGCCCTCCCCAAAACAGTGCTGCCACCATCCACGACTCCTCCCACCCTCCCTCCGCCATCGCCTCAGCTGACTCCACTCTCGGCCGCCACTTAGCACGCCGCCTGGTGGAGATCGGGGTGGGGGACGTGTTCTCTGTCCCCGGGGATTTTAACCTAACACTACTAGACCATCTCATTGCTGAACCGGGGCTCACCAACATCGGCTGCTGCAATGAACTCAATGCCGGGTACGCCGCTGATGGGTACGCCCGGGCCCGTGGCGTCGGGGCGTGCGTTGTCACGTTTACTGTGGGTGGGCTTAGTGTTCTCAATGCCATTGCTGGAGCTTGCAGTGAGAACCTTCCTGTTATTTGTGTTGTTGGAGGGCCTAACACAAATGATTATGGCACGAACAGAATTCTTCACCACACCATTGGCTTGCCGGATTTCAGCCAAGAACTCAGATGCTTCCAGACTGTCACTTGCTATCAAGCTGTGGTGAACAACTTGGACGATGCACATGAACAGATTGACACAGCCATTTCTACTGCTCTCAAAGAAAGCAAGCCTGTATACATTAGCATCAGCTGCAACTTGCCTGCCATTCCACATCCAACTTTTAGCAGAGAGCCCATTAAATTTTGCATCTCACCTAGAATGACCAACAAGATGGGGCTAGACGCCGCTGTGGAGGCAGCAGCTGCCTTCTTGAAGAAGGCAGTGAAGCCAGTTGTGGTTGGCGGACCCAAGCTCCGAGTGGCAAAGGCTCATGAAGCCTTTGTTGAATTTGCAGATGCCAGTGGCTATGCTGTTTCTGTGATGCCATCAGCCAAAGGGCATTTCCCTGAAACACATCCTCGCTACATCGGGACATACTGGGGAGCAGTCGGCACTGCCTACTGCGGTGAGATTGTGGAGTCTGCTGATGCATACGTGTTCGCCAGTCCAATCTTCAATGACTACAGCTCGGTTGGCTACTCTCTCCTTCTCAAAAAGGAGAAGGCAATCATTGTGAAGCCCGATAGCGTGGTGGTTGGCAATGGCCCTTCTTTCGGTTGTGTCCTAATGAAGGACTTCTTCAGGCACTTGCGAAGAGGGTCACCAAAAACACAACCGCTTACGAAAACTACGCCAGAATCTACGTCCCAAATGGCCAGCCTCCCAAATGTCAGCCTAATGAGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

408

Amino Acids

42.97

Weight (kDa)

6.63

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 45 - 150 6.4e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 242 - 359 4.2e-22 Thiamine pyrophosphate enzyme, central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 609
AciI CCGC 8 cut(s) 111, 142, 157, 291, 720, 777, 939, 1151
AcoI YGGCCR 3 cut(s) 3, 139, 1188
AcsI RAATTY 3 cut(s) 453, 674, 821
AcuI CTGAAG 1 cut(s) 1099
AcyI GRCGYC 2 cut(s) 318, 717
AfaI GTAC 2 cut(s) 287, 302
AfiI CCNNNNNNNGG 4 cut(s) 163, 297, 1088, 1105
AflIII ACRYGT 2 cut(s) 183, 969
AgsI TTSAA 4 cut(s) 33, 748, 821, 991
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 159
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 7 cut(s) 125, 383, 530, 624, 737, 787, 1002
AluI AGCT 7 cut(s) 125, 383, 530, 624, 737, 787, 1002
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 2 cut(s) 197, 306
AoxI GGCC 6 cut(s) 3, 139, 309, 422, 1078, 1188
ApaI GGGCCC 1 cut(s) 313
ApeKI GCWGC 7 cut(s) 77, 261, 264, 624, 731, 734, 737
ApoI RAATTY 3 cut(s) 453, 674, 821
Asp700I GAANNNNTTC 1 cut(s) 1109
AspS9I GGNCC 5 cut(s) 309, 310, 422, 779, 1079
AsuC2I CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
AsuHPI GGTGA 6 cut(s) 241, 452, 547, 678, 953, 1128
AvaI CYCGRG 2 cut(s) 197, 306
AvaII GGWCC 1 cut(s) 779
BaeGI GKGCMC 1 cut(s) 313
BalI TGGCCA 2 cut(s) 5, 1190
BanII GRGCYC 3 cut(s) 249, 313, 669
BbvCI CCTCAGC 1 cut(s) 121
BbvI GCAGC 7 cut(s) 64, 248, 251, 611, 724, 743, 746
BccI CCATC 6 cut(s) 92, 122, 234, 290, 700, 868
BciT130I CCWGG 1 cut(s) 161
BcnI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
BfaI CTAG 4 cut(s) 23, 221, 690, 713
BfmI CTRYAG 1 cut(s) 997
BfuAI ACCTGC 1 cut(s) 46
Bme1390I CCNGG 7 cut(s) 161, 198, 199, 243, 283, 307, 308
Bme18I GGWCC 1 cut(s) 779
BmeT110I CYCGRG 2 cut(s) 197, 306
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 5 cut(s) 309, 310, 422, 779, 1079
BmiI GGNNCC 3 cut(s) 19, 311, 781
BmrFI CCNGG 7 cut(s) 161, 198, 199, 243, 283, 307, 308
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 7 cut(s) 491, 541, 627, 690, 820, 846, 952
BmuI ACTGGG 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 121
BpuMI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
BsaAI YACGTR 1 cut(s) 970
BsaHI GRCGYC 2 cut(s) 318, 717
BsaJI CCNNGG 6 cut(s) 196, 197, 198, 242, 306, 313
BsaXI ACNNNNNCTCC 6 cut(s) 80, 92, 110, 122, 771, 801
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 297, 1088, 1105
Bse1I ACTGG 4 cut(s) 764, 834, 917, 978
Bse3DI GCAATG 4 cut(s) 231, 274, 372, 1081
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 6 cut(s) 196, 197, 198, 242, 306, 313
BseGI GGATG 3 cut(s) 84, 646, 889
BseLI CCNNNNNNNGG 4 cut(s) 163, 297, 1088, 1105
BseMI GCAATG 4 cut(s) 231, 274, 372, 1081
BseMII CTCAG 2 cut(s) 135, 511
BseNI ACTGG 4 cut(s) 764, 834, 917, 978
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 313
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 7 cut(s) 64, 248, 251, 611, 724, 743, 746
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 6 cut(s) 5, 141, 311, 424, 1080, 1190
BsiEI CGRYCG 1 cut(s) 142
BsiHKCI CYCGRG 2 cut(s) 197, 306
BsiSI CCGG 5 cut(s) 198, 242, 282, 307, 479
BslFI GGGAC 4 cut(s) 179, 194, 919, 1165
BslI CCNNNNNNNGG 4 cut(s) 163, 297, 1088, 1105
BsmFI GGGAC 4 cut(s) 179, 194, 919, 1165
BsnI GGCC 6 cut(s) 5, 141, 311, 424, 1080, 1190
BsoBI CYCGRG 2 cut(s) 197, 306
Bsp120I GGGCCC 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 249, 313, 669
Bsp143I GATC 1 cut(s) 168
BspACI CCGC 8 cut(s) 111, 142, 157, 291, 720, 777, 939, 1151
BspANI GGCC 6 cut(s) 5, 141, 311, 424, 1080, 1190
BspCNI CTCAG 2 cut(s) 134, 510
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 3 cut(s) 19, 311, 781
BspMI ACCTGC 1 cut(s) 46
BsrDI GCAATG 4 cut(s) 231, 274, 372, 1081
BsrI ACTGG 4 cut(s) 764, 834, 917, 978
BssECI CCNNGG 6 cut(s) 196, 197, 198, 242, 306, 313
BssMI GATC 1 cut(s) 168
BssNAI GTATAC 1 cut(s) 610
BssNI GRCGYC 2 cut(s) 318, 717
Bst1107I GTATAC 1 cut(s) 610
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 74, 346, 514
Bst6I CTCTTC 2 cut(s) 34, 1123
BstACI GRCGYC 2 cut(s) 318, 717
BstAPI GCANNNNNTGC 2 cut(s) 624, 737
BstBAI YACGTR 1 cut(s) 970
BstC8I GCNNGC 7 cut(s) 154, 328, 385, 476, 602, 636, 1192
BstDEI CTNAG 4 cut(s) 121, 148, 356, 497
BstDSI CCRYGG 1 cut(s) 313
BstEII GGTNACC 1 cut(s) 1134
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 3 cut(s) 84, 646, 889
BstKTI GATC 1 cut(s) 171
BstMBI GATC 1 cut(s) 168
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 8 cut(s) 57, 287, 624, 728, 737, 809, 1008, 1215
BstNI CCWGG 1 cut(s) 161
BstPI GGTNACC 1 cut(s) 1134
BstSCI CCNGG 7 cut(s) 159, 196, 197, 241, 281, 305, 306
BstSFI CTRYAG 1 cut(s) 997
BstSLI GKGCMC 1 cut(s) 313
BstV1I GCAGC 7 cut(s) 64, 248, 251, 611, 724, 743, 746
BstXI CCANNNNNNTGG 2 cut(s) 470, 706
BstZ17I GTATAC 1 cut(s) 610
BstZI CGGCCG 1 cut(s) 139
BsuRI GGCC 6 cut(s) 5, 141, 311, 424, 1080, 1190
BtgI CCRYGG 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 100
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 3 cut(s) 84, 646, 889
BtsI GCAGTG 3 cut(s) 394, 762, 927
BtsIMutI CAGTG 5 cut(s) 79, 394, 762, 841, 927
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 7 cut(s) 154, 328, 385, 476, 602, 636, 1192
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 805
Cfr13I GGNCC 5 cut(s) 309, 310, 422, 779, 1079
Cfr9I CCCGGG 2 cut(s) 197, 306
CseI GACGC 3 cut(s) 40, 307, 725
Csp6I GTAC 2 cut(s) 286, 301
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 2 cut(s) 557, 806
CviQI GTAC 2 cut(s) 286, 301
DdeI CTNAG 4 cut(s) 121, 148, 356, 497
DpnI GATC 1 cut(s) 170
DpnII GATC 1 cut(s) 168
EaeI YGGCCR 3 cut(s) 3, 139, 1188
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 34, 1123
EarI CTCTTC 2 cut(s) 34, 1123
EciI GGCGGA 2 cut(s) 100, 792
EclXI CGGCCG 1 cut(s) 139
Eco24I GRGCYC 3 cut(s) 249, 313, 669
Eco47I GGWCC 1 cut(s) 779
Eco52I CGGCCG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 1099
Eco88I CYCGRG 2 cut(s) 197, 306
Eco91I GGTNACC 1 cut(s) 1134
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 1 cut(s) 422
EcoO65I GGTNACC 1 cut(s) 1134
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 3 cut(s) 249, 313, 669
FaeI CATG 2 cut(s) 560, 809
FaiI YATR 7 cut(s) 441, 558, 610, 807, 843, 910, 967
FalI AAGNNNNNCTT 2 cut(s) 1097, 1129
FaqI GGGAC 4 cut(s) 179, 194, 919, 1165
FatI CATG 2 cut(s) 556, 805
FblI GTMKAC 1 cut(s) 609
FokI GGATG 3 cut(s) 71, 633, 876
FriOI GRGCYC 3 cut(s) 249, 313, 669
FspBI CTAG 4 cut(s) 23, 221, 690, 713
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 6 cut(s) 5, 141, 311, 424, 1080, 1190
HapII CCGG 5 cut(s) 198, 242, 282, 307, 479
HgaI GACGC 3 cut(s) 40, 307, 725
Hin1I GRCGYC 2 cut(s) 318, 717
Hin1II CATG 2 cut(s) 560, 809
HinfI GANTC 5 cut(s) 26, 92, 128, 953, 1172
HpaII CCGG 5 cut(s) 198, 242, 282, 307, 479
HphI GGTGA 6 cut(s) 241, 452, 547, 678, 953, 1128
Hpy166II GTNNAC 3 cut(s) 342, 538, 610
Hpy188I TCNGA 3 cut(s) 500, 791, 1223
Hpy188III TCNNGA 4 cut(s) 508, 745, 806, 904
Hpy8I GTNNAC 3 cut(s) 342, 538, 610
Hpy99I CGWCG 2 cut(s) 56, 323
HpyAV CCTTC 7 cut(s) 407, 745, 751, 1030, 1031, 1092, 1099
HpyCH4III ACNGT 3 cut(s) 74, 346, 514
HpyCH4IV ACGT 4 cut(s) 183, 338, 969, 1178
HpyCH4V TGCA 7 cut(s) 267, 387, 554, 627, 681, 827, 965
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 287, 624, 728, 737, 809, 1008, 1215
HpyF3I CTNAG 4 cut(s) 121, 148, 356, 497
HpySE526I ACGT 4 cut(s) 183, 338, 969, 1178
Hsp92I GRCGYC 2 cut(s) 318, 717
Hsp92II CATG 2 cut(s) 560, 809
Kzo9I GATC 1 cut(s) 168
LmnI GCTCC 4 cut(s) 23, 380, 792, 917
Lsp1109I GCAGC 7 cut(s) 64, 248, 251, 611, 724, 743, 746
LweI GCATC 7 cut(s) 491, 541, 627, 690, 820, 846, 952
MaeI CTAG 4 cut(s) 23, 221, 690, 713
MaeII ACGT 4 cut(s) 183, 338, 969, 1178
MaeIII GTNAC 3 cut(s) 334, 514, 1134
MalI GATC 1 cut(s) 170
MboI GATC 1 cut(s) 168
MboII GAAGA 6 cut(s) 21, 449, 760, 979, 1105, 1140
MhlI GDGCHC 3 cut(s) 249, 313, 669
MlsI TGGCCA 2 cut(s) 5, 1190
MluCI AATT 3 cut(s) 453, 674, 821
MluNI TGGCCA 2 cut(s) 5, 1190
MlyI GAGTC 4 cut(s) 20, 86, 122, 962
MmeI TCCRAC 2 cut(s) 397, 674
Mox20I TGGCCA 2 cut(s) 5, 1190
Mph1103I ATGCAT 1 cut(s) 967
MroXI GAANNNNTTC 1 cut(s) 1109
MscI TGGCCA 2 cut(s) 5, 1190
MseI TTAA 2 cut(s) 207, 672
Msp20I TGGCCA 2 cut(s) 5, 1190
MspA1I CMGCKG 5 cut(s) 125, 293, 624, 722, 737
MspI CCGG 5 cut(s) 198, 242, 282, 307, 479
MspR9I CCNGG 7 cut(s) 161, 198, 199, 243, 283, 307, 308
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 8 cut(s) 57, 287, 624, 728, 737, 809, 1008, 1215
NciI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
NdeII GATC 1 cut(s) 168
NlaIII CATG 2 cut(s) 560, 809
NlaIV GGNNCC 3 cut(s) 19, 311, 781
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 3 cut(s) 334, 514, 1134
NsiI ATGCAT 1 cut(s) 967
PagI TCATGA 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 1109
PfeI GAWTC 1 cut(s) 1172
PleI GAGTC 4 cut(s) 20, 86, 122, 961
PpsI GAGTC 4 cut(s) 20, 86, 122, 961
Ppu21I YACGTR 1 cut(s) 970
Psp6I CCWGG 1 cut(s) 159
PspEI GGTNACC 1 cut(s) 1134
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 19, 311, 781
PspOMI GGGCCC 1 cut(s) 309
PspPI GGNCC 5 cut(s) 309, 310, 422, 779, 1079
PstNI CAGNNNCTG 1 cut(s) 737
PvuII CAGCTG 3 cut(s) 125, 624, 737
RsaI GTAC 2 cut(s) 287, 302
RsaNI GTAC 2 cut(s) 286, 301
SaqAI TTAA 2 cut(s) 207, 672
Sau3AI GATC 1 cut(s) 168
Sau96I GGNCC 5 cut(s) 309, 310, 422, 779, 1079
SchI GAGTC 4 cut(s) 20, 86, 122, 962
ScrFI CCNGG 7 cut(s) 161, 198, 199, 243, 283, 307, 308
SduI GDGCHC 3 cut(s) 249, 313, 669
SfaNI GCATC 7 cut(s) 491, 541, 627, 690, 820, 846, 952
SfcI CTRYAG 1 cut(s) 997
SinI GGWCC 1 cut(s) 779
SmaI CCCGGG 2 cut(s) 199, 308
SrfI GCCCGGGC 1 cut(s) 308
Sse9I AATT 3 cut(s) 453, 674, 821
SsiI CCGC 8 cut(s) 111, 142, 157, 291, 720, 777, 939, 1151
SspMI CTAG 4 cut(s) 23, 221, 690, 713
StyD4I CCNGG 7 cut(s) 159, 196, 197, 241, 281, 305, 306
TaaI ACNGT 3 cut(s) 74, 346, 514
TaiI ACGT 4 cut(s) 186, 341, 972, 1181
TasI AATT 3 cut(s) 453, 674, 821
TauI GCSGC 4 cut(s) 144, 159, 293, 722
TfiI GAWTC 1 cut(s) 1172
Tru1I TTAA 2 cut(s) 207, 672
Tru9I TTAA 2 cut(s) 207, 672
TscAI CASTG 5 cut(s) 79, 394, 762, 841, 934
TseFI GTSAC 3 cut(s) 334, 514, 1134
TseI GCWGC 7 cut(s) 77, 261, 264, 624, 731, 734, 737
Tsp45I GTSAC 3 cut(s) 334, 514, 1134
TspDTI ATGAA 4 cut(s) 285, 573, 822, 1118
TspMI CCCGGG 2 cut(s) 197, 306
TspRI CASTG 5 cut(s) 79, 394, 762, 841, 934
VpaK11BI GGWCC 1 cut(s) 779
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 3 cut(s) 453, 674, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XmaI CCCGGG 2 cut(s) 197, 306
XmiI GTMKAC 1 cut(s) 609
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 4 cut(s) 23, 221, 690, 713
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.