Rh3DG356500

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001627
Physical Location & Seq
Reverse (-)
43382717 .. 43383112
396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG356500.1

Sequence Viewer

Length: 396 bp
ATGCTGGGCCGACACCTGGCTTTGCGGTTGGTCGAGATCGGCGTTCATGACATGTTCTCCATCCCCGGCGACTTCAACTTGACCCTTTTGGACCACCTGATCTCCGAGCTGGAGCTCAACCTCATAGGCTGCTACAACGAGCTCAACGCCGAGTACGCCGCCAATGGCTACCCGAGCGCTAGGGGTATCGGCGCGTGTGTCGTCACTTTCACGGTGGGTGGGCTCAGCTTCTTGAACGCTATCGCCGATGCTTGCAGTGAGAACTTGCCGGTGATTTGTATTATCGGTGGGAGAGATCAATTCCTAGTCTTGTCGACGTCTTTTGAATCTGGTTGCTGGGTTTGTAACTCAAAGCAATGCAAGGGTAAAGTTGGAAAGTACATGACAATATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

131

Amino Acids

14.17

Weight (kDa)

5.19

Isoelectric Point (pI)

30.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 3 - 99 9.8e-21 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 314
AccII CGCG 1 cut(s) 194
AciI CCGC 2 cut(s) 25, 159
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 2 cut(s) 155, 380
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 3 cut(s) 76, 235, 326
AjnI CCWGG 1 cut(s) 15
AluBI AGCT 4 cut(s) 109, 115, 142, 228
AluI AGCT 4 cut(s) 109, 115, 142, 228
Alw21I GWGCWC 2 cut(s) 117, 144
Ama87I CYCGRG 1 cut(s) 172
Aor51HI AGCGCT 1 cut(s) 178
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 129
AspLEI GCGC 2 cut(s) 179, 194
AspS9I GGNCC 2 cut(s) 7, 91
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 283
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 1 cut(s) 91
BanII GRGCYC 3 cut(s) 117, 144, 225
Bbv12I GWGCWC 2 cut(s) 117, 144
BbvI GCAGC 1 cut(s) 116
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 17
BcnI CCSGG 1 cut(s) 66
BfaI CTAG 2 cut(s) 180, 305
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 2 cut(s) 130, 159
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 2 cut(s) 131, 160
Bme1390I CCNGG 2 cut(s) 17, 66
Bme18I GGWCC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 7, 91
BmrFI CCNGG 2 cut(s) 17, 66
BmsI GCATC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 131
Bpu1102I GCTNAGC 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 64
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 86, 116
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 362
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 60
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMI GCAATG 1 cut(s) 362
BseMII CTCAG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 116
BseYI CCCAGC 2 cut(s) 4, 336
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 1 cut(s) 9
BsiHKAI GWGCWC 2 cut(s) 117, 144
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 2 cut(s) 66, 269
BslI CCNNNNNNNGG 1 cut(s) 16
BsnI GGCC 1 cut(s) 9
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 3 cut(s) 117, 144, 225
Bsp143I GATC 3 cut(s) 36, 99, 295
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 2 cut(s) 25, 159
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 1 cut(s) 194
BspHI TCATGA 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 362
BsrFI RCCGGY 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 3 cut(s) 36, 99, 295
BssNI GRCGYC 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 214
BstACI GRCGYC 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 60
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 2 cut(s) 179, 194
BstKTI GATC 3 cut(s) 39, 102, 298
BstMBI GATC 3 cut(s) 36, 99, 295
BstMWI GCNNNNNNNGC 2 cut(s) 155, 174
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 15, 64
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 116
BsuRI GGCC 1 cut(s) 9
BtsCI GGATG 1 cut(s) 60
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 253
CciI TCATGA 1 cut(s) 46
CfoI GCGC 2 cut(s) 179, 194
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 7, 91
Csp6I GTAC 2 cut(s) 154, 379
CviAII CATG 3 cut(s) 47, 52, 382
CviJI RGCY 9 cut(s) 9, 20, 109, 115, 129, 142, 168, 223, 228
CviKI_1 RGCY 9 cut(s) 9, 20, 109, 115, 129, 142, 168, 223, 228
CviQI GTAC 2 cut(s) 154, 379
DdeI CTNAG 1 cut(s) 224
DpnI GATC 3 cut(s) 38, 101, 297
DpnII GATC 3 cut(s) 36, 99, 295
Ecl136II GAGCTC 2 cut(s) 115, 142
Eco24I GRGCYC 3 cut(s) 117, 144, 225
Eco47I GGWCC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 178
Eco53kI GAGCTC 2 cut(s) 115, 142
Eco88I CYCGRG 1 cut(s) 172
EcoICRI GAGCTC 2 cut(s) 115, 142
EcoRII CCWGG 1 cut(s) 15
EcoT38I GRGCYC 3 cut(s) 117, 144, 225
FaeI CATG 3 cut(s) 50, 55, 385
FaiI YATR 4 cut(s) 48, 53, 125, 383
FatI CATG 3 cut(s) 46, 51, 381
FblI GTMKAC 1 cut(s) 314
Fnu4HI GCNGC 2 cut(s) 130, 159
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 3 cut(s) 117, 144, 225
Fsp4HI GCNGC 2 cut(s) 130, 159
FspBI CTAG 2 cut(s) 180, 305
GlaI GCGC 2 cut(s) 178, 193
GluI GCNGC 2 cut(s) 130, 159
GsaI CCCAGC 2 cut(s) 8, 340
GsuI CTGGAG 1 cut(s) 131
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 1 cut(s) 9
HapII CCGG 2 cut(s) 66, 269
HhaI GCGC 2 cut(s) 179, 194
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 3 cut(s) 50, 55, 385
Hin6I GCGC 2 cut(s) 177, 192
HinP1I GCGC 2 cut(s) 177, 192
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 1 cut(s) 326
HpaII CCGG 2 cut(s) 66, 269
HphI GGTGA 1 cut(s) 283
Hpy166II GTNNAC 1 cut(s) 315
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 3 cut(s) 34, 47, 232
Hpy8I GTNNAC 1 cut(s) 315
Hpy99I CGWCG 1 cut(s) 319
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 317
HpyCH4V TGCA 2 cut(s) 255, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 174
HpyF3I CTNAG 1 cut(s) 224
HpySE526I ACGT 1 cut(s) 317
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 3 cut(s) 50, 55, 385
HspAI GCGC 2 cut(s) 177, 192
Kzo9I GATC 3 cut(s) 36, 99, 295
LmnI GCTCC 1 cut(s) 112
LpnPI CCDG 8 cut(s) 2, 29, 79, 95, 110, 282, 315, 322
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 1 cut(s) 238
MaeI CTAG 2 cut(s) 180, 305
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 2 cut(s) 202, 344
MalI GATC 3 cut(s) 38, 101, 297
MboI GATC 3 cut(s) 36, 99, 295
MhlI GDGCHC 3 cut(s) 117, 144, 225
MluCI AATT 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 1 cut(s) 131
MseI TTAA 1 cut(s) 394
MspI CCGG 2 cut(s) 66, 269
MspR9I CCNGG 2 cut(s) 17, 66
MvaI CCWGG 1 cut(s) 17
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 2 cut(s) 155, 174
NciI CCSGG 1 cut(s) 66
NdeII GATC 3 cut(s) 36, 99, 295
NlaIII CATG 3 cut(s) 50, 55, 385
NmeAIII GCCGAG 1 cut(s) 175
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 55
PagI TCATGA 1 cut(s) 46
PciI ACATGT 1 cut(s) 51
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 243
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 131, 160
PscI ACATGT 1 cut(s) 51
Psp124BI GAGCTC 2 cut(s) 117, 144
Psp6I CCWGG 1 cut(s) 15
PspFI CCCAGC 2 cut(s) 4, 336
PspGI CCWGG 1 cut(s) 15
PspPI GGNCC 2 cut(s) 7, 91
RsaI GTAC 2 cut(s) 155, 380
RsaNI GTAC 2 cut(s) 154, 379
SacI GAGCTC 2 cut(s) 117, 144
SalI GTCGAC 1 cut(s) 313
SaqAI TTAA 1 cut(s) 394
SatI GCNGC 2 cut(s) 130, 159
Sau3AI GATC 3 cut(s) 36, 99, 295
Sau96I GGNCC 2 cut(s) 7, 91
ScrFI CCNGG 2 cut(s) 17, 66
SduI GDGCHC 3 cut(s) 117, 144, 225
SetI ASST 8 cut(s) 18, 99, 111, 117, 123, 144, 230, 320
SfaNI GCATC 1 cut(s) 238
SinI GGWCC 1 cut(s) 91
Sse9I AATT 1 cut(s) 299
SsiI CCGC 2 cut(s) 25, 159
SspI AATATT 1 cut(s) 390
SspMI CTAG 2 cut(s) 180, 305
SstI GAGCTC 2 cut(s) 117, 144
StyD4I CCNGG 2 cut(s) 15, 64
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 320
TaqI TCGA 2 cut(s) 33, 314
TasI AATT 1 cut(s) 299
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 394
Tru9I TTAA 1 cut(s) 394
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 91
XceI RCATGY 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 314
XspI CTAG 2 cut(s) 180, 305
ZraI GACGTC 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.