Rh5AG080500

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001627
Physical Location & Seq
Forward (+)
6876380 .. 6880100
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG080500.1

Sequence Viewer

Length: 1773 bp
ATGGAACCTTCTACACTCATCGGTGCGACGCCTCGGCCCACCTCGGCTCTACTTCCGGTGGTCCCCGCCGCCGCCTGCAGCGGCACGCTGGGCCGCCACCTGGCTCGGCGGCTGGTCGAGATCGGCGTTCATGACGTGTTCTCAGTCCCCGGCGACTTCAACTTGACCCTCTTGGACCACCTGATCGCCGAGCCGGAGCTCAACCTCATCGGCTGCTGCAACGAGCTCAACGCCGGCTACGCCGCCGACGGCTACGCGCGCGCCAGGGGCGTCGGCGCGTGTGTCGTCACTTTCACGGTGGGTGGGCTCAGCGTCTTGAACGCCATCGCCGGTGCTTACAGTGAGAACTTGCCGGTGATTTGTATCGTCGGTGGGCCCAACTCCAATGATTACGGGACCAATCGGATCCTCCACCACACGATCGGGTTGCCCGATTTCTCTCAGGAGCTCCGGTGCTTCCAAACTGTTACTTGTCACCAGGCAGTGGTGAGTCATTTGGAAGATGCGCATGAGCTTATAGACACGGCAATTTCGACAGCATTGAAGGAGAGCAAGCCGGTTTACATTAGCATAAGTTGTAATTTATCTGCAATTCCTCACCCCACATTTGGTAGAGACCCTGTTCCCTTCTTTCTTGCACCCAAAGTAAGCAATCAATTAGGGTTGGAGGCAGCTGTTGAAGCAACTGCTGCATTCCTGAACAAGGCAGTGAAGCCTGTGCTTGTGGGTGGGCCCAAGCTGAGAGTGGCAAAGGCCCAGAAGGCCTTTGTGGAGCTTGCAGATGCTAGTGGGTACCCAATAGCGGTTATGCCCTCCGGTAAGGGTTTGGTGCCGGAGCACCACCCACATTTCATTGGGACCTATTGGGGTGCTGTGAGTACTAGTTTCTGTGGAGAGATTGTGGAGTCTGCAGATGCTTATGTTTTTGTTGGCCCAATTTTTAATGATTATAGCTCTGTTGGGTACTCCTTGCTGATCAAGAAGGAGAAGGCAATCATTGTGGAGCCTAATCGCGTGACCATTGGGAATGGTCCATCATTTGGCTGGGTTTTCATGGGTGATTTCTTGACTGCATTGGCCAAGAAGCTGAAGAAAAACAGTACCGGTATGGAGAATTACCGCAGAATTTTTGTTCCCCATGGAATCCCTCTGAAAGCTCAGAAAGATGAATCTCTTAGAGTAAATGTTCTGTTTAAGCACATTCAGGAGATTGTCACCCCAAACACTGCCATAATTGCCGAAACTGGTGACTCATGGTTCAATTGTCAGAAGCTCCGCCTCCCTGAGAATTGTGGGTATGAATTCCAGATGCAATATGGATCTATTGGATGGTCAGTCGGTGCTACTCTCGGTTATGCTCAGGGTGCCAAAGATAAACGTGTAATTGCTTGCATTGGTGATGGGAGTTTCCAGGTGACTGCCCAGGACATTTCAACAATGATCCGATGTGGACAAAATACCATTATATTTCTTATCAACAATGGAGGCTATACAATTGAAGTAGAGATTCATGATGGCCCATACAATGTCATTAAGAACTGGGATTATACTGGTCTTGTTGATGCAATCCACAATGGTGAAGGCAAATGCTGGACTACCAAGGTACGTACAGAGGAGGACTTAACTGAAGCAATTGCAAAAGCAACAGGGGAGCACAAGGATAGTTTATGCTTCATCGAAGTTTTTGTGCACAAAGATGACACTAGCAAAGAGCTTCTGGAATGGGGATCCCGAGTTTCAGCTGCTAACAGCCGACCTCCAAATCCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

590

Amino Acids

63.7

Weight (kDa)

5.99

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 29 - 134 1.8e-29 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 226 - 344 1.6e-23 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 436 - 561 4.2e-15 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 507
Acc65I GGTACC 1 cut(s) 792
AccB1I GGYRCC 3 cut(s) 792, 829, 1364
AccB7I CCANNNNNTGG 2 cut(s) 484, 1040
AccII CGCG 5 cut(s) 257, 259, 261, 278, 1014
AclWI GGATC 6 cut(s) 400, 413, 1327, 1435, 1722, 1735
AcoI YGGCCR 1 cut(s) 1077
AcsI RAATTY 2 cut(s) 1125, 1301
AcuI CTGAAG 2 cut(s) 1109, 1647
AcyI GRCGYC 2 cut(s) 29, 270
AfaI GTAC 6 cut(s) 794, 880, 965, 1102, 1605, 1609
AfiI CCNNNNNNNGG 6 cut(s) 100, 484, 608, 703, 802, 1040
AflIII ACRYGT 2 cut(s) 135, 1378
AgeI ACCGGT 1 cut(s) 1103
AgsI TTSAA 7 cut(s) 160, 319, 544, 680, 1261, 1434, 1499
AhlI ACTAGT 1 cut(s) 881
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 5 cut(s) 99, 263, 477, 1410, 1422
AleI CACNNNNGTG 1 cut(s) 1575
Alw21I GWGCWC 6 cut(s) 201, 228, 450, 840, 1656, 1692
Alw26I GTCTC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 1688
AlwI GGATC 6 cut(s) 400, 413, 1327, 1435, 1722, 1735
Ama87I CYCGRG 1 cut(s) 1731
AoxI GGCC 9 cut(s) 35, 91, 374, 731, 753, 762, 931, 1077, 1516
ApaI GGGCCC 2 cut(s) 378, 735
ApaLI GTGCAC 1 cut(s) 1688
ApeKI GCWGC 6 cut(s) 78, 213, 216, 671, 689, 1742
ApoI RAATTY 2 cut(s) 1125, 1301
AsiGI ACCGGT 1 cut(s) 1103
Asp718I GGTACC 1 cut(s) 792
AspLEI GCGC 5 cut(s) 259, 261, 263, 278, 508
AsuC2I CCSGG 1 cut(s) 150
AvaI CYCGRG 1 cut(s) 1731
AvaII GGWCC 5 cut(s) 61, 175, 396, 858, 1031
BaeGI GKGCMC 3 cut(s) 378, 735, 1692
BalI TGGCCA 1 cut(s) 1079
BamHI GGATCC 2 cut(s) 405, 1727
BanI GGYRCC 3 cut(s) 792, 829, 1364
BanII GRGCYC 6 cut(s) 201, 228, 309, 378, 450, 735
Bbv12I GWGCWC 6 cut(s) 201, 228, 450, 840, 1656, 1692
BbvI GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1729
BccI CCATC 5 cut(s) 332, 1042, 1323, 1394, 1508
BceAI ACGGC 2 cut(s) 265, 540
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 5 cut(s) 101, 265, 479, 1412, 1424
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 609
BcuI ACTAGT 1 cut(s) 881
BfaI CTAG 3 cut(s) 786, 882, 1704
BfmI CTRYAG 2 cut(s) 76, 909
BglI GCCNNNNNGGC 1 cut(s) 761
BlpI GCTNAGC 1 cut(s) 308
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 6 cut(s) 101, 150, 265, 479, 1412, 1424
Bme18I GGWCC 5 cut(s) 61, 175, 396, 858, 1031
BmeT110I CYCGRG 1 cut(s) 1731
BmgBI CACGTC 1 cut(s) 136
BmrFI CCNGG 6 cut(s) 101, 150, 265, 479, 1412, 1424
BmrI ACTGGG 1 cut(s) 1549
BmsI GCATC 5 cut(s) 493, 772, 904, 1299, 1552
BmuI ACTGGG 1 cut(s) 1549
Bpu10I CCTNAGC 1 cut(s) 1359
Bpu1102I GCTNAGC 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 1607
BsaBI GATNNNNATC 1 cut(s) 362
BsaHI GRCGYC 2 cut(s) 29, 270
BsaI GGTCTC 1 cut(s) 609
BsaJI CCNNGG 7 cut(s) 32, 42, 148, 264, 1138, 1422, 1599
BsaWI WCCGGW 4 cut(s) 55, 450, 815, 1103
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 885, 915
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 484, 608, 703, 802, 1040
Bse118I RCCGGY 5 cut(s) 233, 329, 352, 556, 1103
Bse1I ACTGG 3 cut(s) 1249, 1544, 1555
Bse8I GATNNNNATC 1 cut(s) 362
BseBI CCWGG 5 cut(s) 101, 265, 479, 1412, 1424
BseDI CCNNGG 7 cut(s) 32, 42, 148, 264, 1138, 1422, 1599
BseGI GGATG 1 cut(s) 1334
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 6 cut(s) 100, 484, 608, 703, 802, 1040
BseMII CTCAG 7 cut(s) 156, 322, 455, 731, 1172, 1275, 1373
BseNI ACTGG 3 cut(s) 1249, 1544, 1555
BsePI GCGCGC 2 cut(s) 257, 259
BseRI GAGGAG 1 cut(s) 1628
BseSI GKGCMC 3 cut(s) 378, 735, 1692
BseXI GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1729
BseYI CCCAGC 2 cut(s) 88, 1044
Bsh1236I CGCG 5 cut(s) 257, 259, 261, 278, 1014
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 9 cut(s) 37, 93, 376, 733, 755, 764, 933, 1079, 1518
BshNI GGYRCC 3 cut(s) 792, 829, 1364
BshTI ACCGGT 1 cut(s) 1103
BsiEI CGRYCG 1 cut(s) 423
BsiHKAI GWGCWC 6 cut(s) 201, 228, 450, 840, 1656, 1692
BsiHKCI CYCGRG 1 cut(s) 1731
BslFI GGGAC 4 cut(s) 47, 131, 409, 871
BslI CCNNNNNNNGG 6 cut(s) 100, 484, 608, 703, 802, 1040
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 4 cut(s) 47, 131, 409, 871
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 9 cut(s) 37, 93, 376, 733, 755, 764, 933, 1079, 1518
Bso31I GGTCTC 1 cut(s) 609
BsoBI CYCGRG 1 cut(s) 1731
Bsp120I GGGCCC 2 cut(s) 374, 731
Bsp1286I GDGCHC 9 cut(s) 201, 228, 309, 378, 450, 735, 840, 1656, 1692
Bsp143I GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
Bsp1720I GCTNAGC 1 cut(s) 308
Bsp19I CCATGG 1 cut(s) 1138
BspANI GGCC 9 cut(s) 37, 93, 376, 733, 755, 764, 933, 1079, 1518
BspCNI CTCAG 7 cut(s) 155, 321, 454, 732, 1171, 1276, 1372
BspFNI CGCG 5 cut(s) 257, 259, 261, 278, 1014
BspHI TCATGA 2 cut(s) 130, 1510
BspMAI CTGCAG 2 cut(s) 80, 913
BspPI GGATC 6 cut(s) 400, 413, 1327, 1435, 1722, 1735
BspT107I GGYRCC 3 cut(s) 792, 829, 1364
BspTNI GGTCTC 1 cut(s) 609
BsrFI RCCGGY 5 cut(s) 233, 329, 352, 556, 1103
BsrI ACTGG 3 cut(s) 1249, 1544, 1555
BssAI RCCGGY 5 cut(s) 233, 329, 352, 556, 1103
BssECI CCNNGG 7 cut(s) 32, 42, 148, 264, 1138, 1422, 1599
BssHII GCGCGC 2 cut(s) 257, 259
BssMI GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
BssNI GRCGYC 2 cut(s) 29, 270
BssT1I CCWWGG 2 cut(s) 1138, 1599
Bst2UI CCWGG 5 cut(s) 101, 265, 479, 1412, 1424
Bst4CI ACNGT 4 cut(s) 298, 341, 466, 1100
BstACI GRCGYC 2 cut(s) 29, 270
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 1607
BstC8I GCNNGC 8 cut(s) 76, 86, 235, 259, 261, 554, 777, 1390
BstDEI CTNAG 9 cut(s) 142, 308, 441, 740, 1158, 1175, 1284, 1359, 1767
BstDSI CCRYGG 1 cut(s) 1138
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 1 cut(s) 1334
BstFNI CGCG 5 cut(s) 257, 259, 261, 278, 1014
BstHHI GCGC 5 cut(s) 259, 261, 263, 278, 508
BstKTI GATC 8 cut(s) 123, 186, 408, 423, 978, 1322, 1443, 1730
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 9 cut(s) 90, 239, 258, 267, 680, 689, 761, 1235, 1364
BstNI CCWGG 5 cut(s) 101, 265, 479, 1412, 1424
BstSCI CCNGG 6 cut(s) 99, 148, 263, 477, 1410, 1422
BstSFI CTRYAG 2 cut(s) 76, 909
BstSLI GKGCMC 3 cut(s) 378, 735, 1692
BstSNI TACGTA 1 cut(s) 1607
BstUI CGCG 5 cut(s) 257, 259, 261, 278, 1014
BstV1I GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1729
BstX2I RGATCY 3 cut(s) 405, 1319, 1727
BstYI RGATCY 3 cut(s) 405, 1319, 1727
BsuRI GGCC 9 cut(s) 37, 93, 376, 733, 755, 764, 933, 1079, 1518
BtgI CCRYGG 1 cut(s) 1138
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 1334
BtsI GCAGTG 3 cut(s) 489, 714, 1224
BtsIMutI CAGTG 4 cut(s) 346, 489, 714, 1224
Cac8I GCNNGC 8 cut(s) 76, 86, 235, 259, 261, 554, 777, 1390
CciI TCATGA 2 cut(s) 130, 1510
CfoI GCGC 5 cut(s) 259, 261, 263, 278, 508
Cfr10I RCCGGY 5 cut(s) 233, 329, 352, 556, 1103
CseI GACGC 3 cut(s) 37, 259, 301
Csp6I GTAC 6 cut(s) 793, 879, 964, 1101, 1604, 1608
CspAI ACCGGT 1 cut(s) 1103
CspCI CAANNNNNGTGG 2 cut(s) 981, 1016
CviAII CATG 6 cut(s) 131, 509, 1054, 1139, 1254, 1511
CviQI GTAC 6 cut(s) 793, 879, 964, 1101, 1604, 1608
DdeI CTNAG 9 cut(s) 142, 308, 441, 740, 1158, 1175, 1284, 1359, 1767
DpnI GATC 8 cut(s) 122, 185, 407, 422, 977, 1321, 1442, 1729
DpnII GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
EaeI YGGCCR 1 cut(s) 1077
EciI GGCGGA 1 cut(s) 1265
Ecl136II GAGCTC 3 cut(s) 199, 226, 448
Eco105I TACGTA 1 cut(s) 1607
Eco130I CCWWGG 2 cut(s) 1138, 1599
Eco147I AGGCCT 1 cut(s) 764
Eco24I GRGCYC 6 cut(s) 201, 228, 309, 378, 450, 735
Eco31I GGTCTC 1 cut(s) 609
Eco47I GGWCC 5 cut(s) 61, 175, 396, 858, 1031
Eco53kI GAGCTC 3 cut(s) 199, 226, 448
Eco57I CTGAAG 2 cut(s) 1109, 1647
Eco88I CYCGRG 1 cut(s) 1731
EcoICRI GAGCTC 3 cut(s) 199, 226, 448
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoO109I RGGNCCY 1 cut(s) 858
EcoRI GAATTC 1 cut(s) 1301
EcoRII CCWGG 5 cut(s) 99, 263, 477, 1410, 1422
EcoT14I CCWWGG 2 cut(s) 1138, 1599
EcoT38I GRGCYC 6 cut(s) 201, 228, 309, 378, 450, 735
ErhI CCWWGG 2 cut(s) 1138, 1599
FaeI CATG 6 cut(s) 134, 512, 1057, 1142, 1257, 1514
FaqI GGGAC 4 cut(s) 47, 131, 409, 871
FatI CATG 6 cut(s) 130, 508, 1053, 1138, 1253, 1510
FauI CCCGC 1 cut(s) 73
FbaI TGATCA 1 cut(s) 975
FokI GGATG 1 cut(s) 1341
FriOI GRGCYC 6 cut(s) 201, 228, 309, 378, 450, 735
FspAI RTGCGCAY 1 cut(s) 507
FspBI CTAG 3 cut(s) 786, 882, 1704
FspI TGCGCA 1 cut(s) 507
GlaI GCGC 5 cut(s) 258, 260, 262, 277, 507
GsaI CCCAGC 2 cut(s) 92, 1048
HaeIII GGCC 9 cut(s) 37, 93, 376, 733, 755, 764, 933, 1079, 1518
HgaI GACGC 3 cut(s) 37, 259, 301
HhaI GCGC 5 cut(s) 259, 261, 263, 278, 508
Hin1I GRCGYC 2 cut(s) 29, 270
Hin1II CATG 6 cut(s) 134, 512, 1057, 1142, 1257, 1514
Hin6I GCGC 5 cut(s) 257, 259, 261, 276, 506
HinP1I GCGC 5 cut(s) 257, 259, 261, 276, 506
HinfI GANTC 6 cut(s) 490, 905, 1143, 1169, 1250, 1507
Hpy166II GTNNAC 3 cut(s) 562, 1451, 1690
Hpy188I TCNGA 5 cut(s) 405, 1152, 1161, 1269, 1445
Hpy8I GTNNAC 3 cut(s) 562, 1451, 1690
Hpy99I CGWCG 4 cut(s) 31, 251, 275, 371
HpyAV CCTTC 7 cut(s) 18, 538, 637, 754, 976, 982, 1574
HpyCH4III ACNGT 4 cut(s) 298, 341, 466, 1100
HpyCH4IV ACGT 3 cut(s) 135, 1378, 1606
HpyF10VI GCNNNNNNNGC 9 cut(s) 90, 239, 258, 267, 680, 689, 761, 1235, 1364
HpyF3I CTNAG 9 cut(s) 142, 308, 441, 740, 1158, 1175, 1284, 1359, 1767
HpySE526I ACGT 3 cut(s) 135, 1378, 1606
Hsp92I GRCGYC 2 cut(s) 29, 270
Hsp92II CATG 6 cut(s) 134, 512, 1057, 1142, 1257, 1514
HspAI GCGC 5 cut(s) 257, 259, 261, 276, 506
KpnI GGTACC 1 cut(s) 796
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
LmnI GCTCC 8 cut(s) 196, 445, 453, 772, 835, 1003, 1278, 1651
Lsp1109I GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1729
LweI GCATC 5 cut(s) 493, 772, 904, 1299, 1552
MaeI CTAG 3 cut(s) 786, 882, 1704
MaeII ACGT 3 cut(s) 135, 1378, 1606
MaeIII GTNAC 7 cut(s) 286, 466, 473, 1015, 1213, 1247, 1414
MalI GATC 8 cut(s) 122, 185, 407, 422, 977, 1321, 1442, 1729
MauBI CGCGCGCG 1 cut(s) 257
MboI GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
MboII GAAGA 2 cut(s) 512, 1102
MfeI CAATTG 3 cut(s) 1261, 1494, 1632
MflI RGATCY 3 cut(s) 405, 1319, 1727
MhlI GDGCHC 9 cut(s) 201, 228, 309, 378, 450, 735, 840, 1656, 1692
MlsI TGGCCA 1 cut(s) 1079
MluNI TGGCCA 1 cut(s) 1079
MlyI GAGTC 3 cut(s) 499, 914, 1244
MmeI TCCRAC 1 cut(s) 645
Mox20I TGGCCA 1 cut(s) 1079
MroNI GCCGGC 1 cut(s) 233
MscI TGGCCA 1 cut(s) 1079
MseI TTAA 4 cut(s) 942, 1194, 1533, 1622
MslI CAYNNNNRTG 2 cut(s) 1575, 1695
Msp20I TGGCCA 1 cut(s) 1079
MspA1I CMGCKG 3 cut(s) 81, 674, 1742
MspR9I CCNGG 6 cut(s) 101, 150, 265, 479, 1412, 1424
MunI CAATTG 3 cut(s) 1261, 1494, 1632
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 5 cut(s) 101, 265, 479, 1412, 1424
MvnI CGCG 5 cut(s) 257, 259, 261, 278, 1014
MwoI GCNNNNNNNGC 9 cut(s) 90, 239, 258, 267, 680, 689, 761, 1235, 1364
NaeI GCCGGC 1 cut(s) 235
NciI CCSGG 1 cut(s) 150
NcoI CCATGG 1 cut(s) 1138
NdeII GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 6 cut(s) 134, 512, 1057, 1142, 1257, 1514
NmeAIII GCCGAG 4 cut(s) 13, 23, 85, 214
NmuCI GTSAC 6 cut(s) 286, 473, 1015, 1213, 1247, 1414
NsbI TGCGCA 1 cut(s) 507
OliI CACNNNNGTG 1 cut(s) 1575
PagI TCATGA 2 cut(s) 130, 1510
PauI GCGCGC 2 cut(s) 257, 259
PceI AGGCCT 1 cut(s) 764
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 429, 530
PctI GAATGC 1 cut(s) 692
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 1143, 1169, 1507
PflMI CCANNNNNTGG 2 cut(s) 484, 1040
PinAI ACCGGT 1 cut(s) 1103
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 3 cut(s) 498, 913, 1244
PpsI GAGTC 3 cut(s) 498, 913, 1244
Ppu21I YACGTR 1 cut(s) 1607
PpuMI RGGWCCY 1 cut(s) 858
Psp124BI GAGCTC 3 cut(s) 201, 228, 450
Psp5II RGGWCCY 1 cut(s) 858
Psp6I CCWGG 5 cut(s) 99, 263, 477, 1410, 1422
PspFI CCCAGC 2 cut(s) 88, 1044
PspGI CCWGG 5 cut(s) 99, 263, 477, 1410, 1422
PspOMI GGGCCC 2 cut(s) 374, 731
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 2 cut(s) 80, 913
PsuI RGATCY 3 cut(s) 405, 1319, 1727
PteI GCGCGC 2 cut(s) 257, 259
PvuI CGATCG 1 cut(s) 423
PvuII CAGCTG 2 cut(s) 674, 1742
RsaI GTAC 6 cut(s) 794, 880, 965, 1102, 1605, 1609
RsaNI GTAC 6 cut(s) 793, 879, 964, 1101, 1604, 1608
RseI CAYNNNNRTG 2 cut(s) 1575, 1695
SacI GAGCTC 3 cut(s) 201, 228, 450
SaqAI TTAA 4 cut(s) 942, 1194, 1533, 1622
Sau3AI GATC 8 cut(s) 120, 183, 405, 420, 975, 1319, 1440, 1727
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 3 cut(s) 499, 914, 1244
ScrFI CCNGG 6 cut(s) 101, 150, 265, 479, 1412, 1424
SduI GDGCHC 9 cut(s) 201, 228, 309, 378, 450, 735, 840, 1656, 1692
SfaNI GCATC 5 cut(s) 493, 772, 904, 1299, 1552
SfcI CTRYAG 2 cut(s) 76, 909
SfiI GGCCNNNNNGGCC 1 cut(s) 761
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 5 cut(s) 61, 175, 396, 858, 1031
SmiMI CAYNNNNRTG 2 cut(s) 1575, 1695
SnaBI TACGTA 1 cut(s) 1607
SpeI ACTAGT 1 cut(s) 881
SseBI AGGCCT 1 cut(s) 764
SspMI CTAG 3 cut(s) 786, 882, 1704
SstI GAGCTC 3 cut(s) 201, 228, 450
StuI AGGCCT 1 cut(s) 764
StyD4I CCNGG 6 cut(s) 99, 148, 263, 477, 1410, 1422
StyI CCWWGG 2 cut(s) 1138, 1599
TaaI ACNGT 4 cut(s) 298, 341, 466, 1100
TaiI ACGT 3 cut(s) 138, 1381, 1609
TaqI TCGA 3 cut(s) 117, 533, 1677
TatI WGTACW 1 cut(s) 878
TauI GCSGC 6 cut(s) 71, 74, 84, 96, 112, 245
TfiI GAWTC 3 cut(s) 1143, 1169, 1507
Tru1I TTAA 4 cut(s) 942, 1194, 1533, 1622
Tru9I TTAA 4 cut(s) 942, 1194, 1533, 1622
TscAI CASTG 4 cut(s) 346, 489, 714, 1231
TseFI GTSAC 6 cut(s) 286, 473, 1015, 1213, 1247, 1414
TseI GCWGC 6 cut(s) 78, 213, 216, 671, 689, 1742
Tsp45I GTSAC 6 cut(s) 286, 473, 1015, 1213, 1247, 1414
TspDTI ATGAA 7 cut(s) 119, 841, 1042, 1182, 1314, 1499, 1663
TspRI CASTG 4 cut(s) 346, 489, 714, 1231
Van91I CCANNNNNTGG 2 cut(s) 484, 1040
VneI GTGCAC 1 cut(s) 1688
VpaK11BI GGWCC 5 cut(s) 61, 175, 396, 858, 1031
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 2 cut(s) 1125, 1301
XcmI CCANNNNNNNNNTGG 3 cut(s) 742, 1041, 1313
XspI CTAG 3 cut(s) 786, 882, 1704
ZrmI AGTACT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.