RLG00000031729

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001627
Physical Location & Seq
Forward (+)
7837465 .. 7840174
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031729

Sequence Viewer

Length: 1770 bp
ATGGAACCTTCCACACTCATCGGTGCAACGCCTCGGCCCACCTCGGCTCCACTTCCGGTGGTCCCAGCCGGCTGCAACGGCACGATGGGCCGCCACCTGGCTCGGCGGCTGGTCGAGATCGGCGTTCTTGACGTGTTCTCGGTCCCCGGCGACTTCAACTTGACCCTCTTGGACCACCTGATCGCCGAGCCGGAGCTCAACCTCATCGGCTGCTGCAACGAGCTCAACGCCGGCTACGCCGCCGACGGCTACGCGCGCGCCGGGGGCGTCGGCGCGTGTGTCGTCACTTTCACGGTGGGTGGGCTCAGCGTCTTGAACGCTATCGCCGGTGCTTACAGTGAGAACTTGCCGGTGATTTGTATCGTCGGTGGGCCCAACTCCAATGATTACGGGACCAATCGGATCCTCCACCACACGATCGGGTTGCCCGATTTCACTCAGGAGCTCCGGTGCTTCCAAACTGTTACTTGTCACCAGGCAGTGGTGAGTCATTTGGAAGATGCGCATGAGCTTATAGACACGGCAATTTCGACAGCATTGAAGGAGAGCAAGCCGGTTTACATTAGCATAAGTTGTAATTTGCCTGCAATTCCTCACCCCACATTTGGTAGAGACCCTGTCCCTTTCTTTCTTGCGCCGAAAGTAAGCAATCAGTTAGGGTTGGAGGCAGCTGTTGAAGCAACTGTTGCATTCCTGAACAAGGCAGTGAAGCCTGTGCTTGTGGGTGGGCCCAAGCTGAGAGTGGCAAAGGCCCAGAAGGCCTTTGTCGAGCTTGCAGATGCTAGTGGGTACCCAATAGCGATTATGCCCTCCGGTAAGGGTTTGGTGCCGGAGCACCACCCACATTTCATTGGGACTTATTGGGGTGCTGTGAGTACTAGTTTCTGTGGAGAGATTGTGGAGTCTGCAGATGCTTATGTTTTTGTTGGCCCAATTTTTAATGATTATAGCTCTGTTGGGTACTCCTTGCTGATCAAGAAGGAAAAGGCAATCATGGTGGAGCCTAATCGCGTGACCATTGGGAATGGTCCATCATTTGGCTGGGTTTTCATGGGTGATTTCTTGACTGCATTGGCCAAGAAGCTGAAGAAAAACAGTACCGGTATGGAGAATTACCTCAGAATTTTCGTTCCCCCTGGAATCCCTCGGAAAGCTCAGAAAGATGAACCTCTTAGAGTAAATGTTCTGTTTAAGCACATTCAGGAGATTGTCACCCCAAACACTGCCATAATCGCCGAAACTGGTGACTCATGGTTCAATTGTCAGAAGCTCCGCCTCCCTGAGAATTGTGGGTACGAATTCCAGATGCAATATGGATCTATTGGATGGTCTGTGGGTGCTACTCTCGGCTATGCTCAGGGTGCCAAAGATAAACGTGTAATTGCTTGCATAGGTGATGGGAGTTTCCAGGTTACTGCCCAGGACATTTCAACAATGATCCGATGTGGACAAAATACCATCATATTTCTTATCAACAATGGTGGCTATACAATCGAAGTAGAGATTCATGATGGCCCATACAATGTCATTAAGAACTGGGATTATACTGGTCTTGTTGATGCAATCCACAATGGTGAAGGCAAATGCTGGACTACCAAGGTACGTACAGAGGAGGACTTAACTGAAGCAATTGCCAAAGCAACAGGGCAGCACAAGGATAGTTTATGCTTCATCGAAGTTTTTGCGCACAAAGATGACACTAGCAAAGAGCTTCTGGAATGGGGTTCCCGAGTTTCAGCTGCTAACAGCCGACCTCCAAATCCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

590

Amino Acids

63.54

Weight (kDa)

5.91

Isoelectric Point (pI)

27.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 28 - 133 8.5e-29 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 225 - 343 3.5e-23 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 435 - 560 4.2e-15 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 504, 1686
Acc65I GGTACC 1 cut(s) 789
AccB1I GGYRCC 3 cut(s) 789, 826, 1361
AccB7I CCANNNNNTGG 2 cut(s) 481, 1037
AccII CGCG 5 cut(s) 254, 256, 258, 275, 1011
AciI CCGC 4 cut(s) 91, 106, 240, 1273
AclWI GGATC 4 cut(s) 397, 410, 1324, 1432
AcoI YGGCCR 1 cut(s) 1074
AcsI RAATTY 2 cut(s) 1122, 1298
AcuI CTGAAG 2 cut(s) 1106, 1644
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 7 cut(s) 791, 877, 962, 1099, 1295, 1602, 1606
AfiI CCNNNNNNNGG 5 cut(s) 97, 481, 605, 700, 1037
AflIII ACRYGT 2 cut(s) 132, 1375
AgeI ACCGGT 1 cut(s) 1100
AgsI TTSAA 6 cut(s) 157, 316, 541, 677, 1258, 1431
AhlI ACTAGT 1 cut(s) 878
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 5 cut(s) 96, 474, 1135, 1407, 1419
AleI CACNNNNGTG 1 cut(s) 1572
Alw21I GWGCWC 4 cut(s) 198, 225, 447, 837
Alw26I GTCTC 1 cut(s) 606
AlwI GGATC 4 cut(s) 397, 410, 1324, 1432
Ama87I CYCGRG 1 cut(s) 1728
AoxI GGCC 9 cut(s) 35, 88, 371, 728, 750, 759, 928, 1074, 1513
ApaI GGGCCC 2 cut(s) 375, 732
ApeKI GCWGC 6 cut(s) 72, 210, 213, 668, 1648, 1739
ApoI RAATTY 2 cut(s) 1122, 1298
AsiGI ACCGGT 1 cut(s) 1100
Asp718I GGTACC 1 cut(s) 789
AspLEI GCGC 7 cut(s) 256, 258, 260, 275, 505, 637, 1687
AsuC2I CCSGG 2 cut(s) 147, 262
AsuHPI GGTGA 9 cut(s) 364, 464, 496, 587, 1067, 1204, 1256, 1406, 1586
AvaI CYCGRG 1 cut(s) 1728
AvaII GGWCC 5 cut(s) 61, 142, 172, 393, 1028
BaeGI GKGCMC 2 cut(s) 375, 732
BalI TGGCCA 1 cut(s) 1076
BamHI GGATCC 1 cut(s) 402
BanI GGYRCC 3 cut(s) 789, 826, 1361
BanII GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Bbv12I GWGCWC 4 cut(s) 198, 225, 447, 837
BbvI GCAGC 6 cut(s) 59, 197, 200, 680, 1660, 1726
BccI CCATC 6 cut(s) 79, 1039, 1320, 1391, 1466, 1505
BceAI ACGGC 3 cut(s) 94, 262, 537
BcgI CGANNNNNNTGC 4 cut(s) 406, 440, 1664, 1698
BciT130I CCWGG 5 cut(s) 98, 476, 1137, 1409, 1421
BclI TGATCA 1 cut(s) 972
BcnI CCSGG 2 cut(s) 147, 262
BcoDI GTCTC 1 cut(s) 606
BcuI ACTAGT 1 cut(s) 878
BfaI CTAG 3 cut(s) 783, 879, 1701
BfmI CTRYAG 1 cut(s) 906
BglI GCCNNNNNGGC 1 cut(s) 758
BisI GCNGC 9 cut(s) 73, 91, 107, 211, 214, 240, 669, 1649, 1740
BlpI GCTNAGC 1 cut(s) 305
BlsI GCNGC 9 cut(s) 74, 92, 108, 212, 215, 241, 670, 1650, 1741
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 7 cut(s) 98, 147, 262, 476, 1137, 1409, 1421
Bme18I GGWCC 5 cut(s) 61, 142, 172, 393, 1028
BmeT110I CYCGRG 1 cut(s) 1728
BmgBI CACGTC 1 cut(s) 133
BmrFI CCNGG 7 cut(s) 98, 147, 262, 476, 1137, 1409, 1421
BmrI ACTGGG 1 cut(s) 1546
BmsI GCATC 5 cut(s) 490, 769, 901, 1296, 1549
BmuI ACTGGG 1 cut(s) 1546
Bpu10I CCTNAGC 1 cut(s) 1356
Bpu1102I GCTNAGC 1 cut(s) 305
BpuMI CCSGG 2 cut(s) 147, 262
BsaAI YACGTR 1 cut(s) 1604
BsaBI GATNNNNATC 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 606
BsaJI CCNNGG 8 cut(s) 32, 42, 145, 261, 1135, 1145, 1419, 1596
BsaWI WCCGGW 4 cut(s) 55, 447, 812, 1100
BsaXI ACNNNNNCTCC 6 cut(s) 31, 61, 434, 464, 882, 912
Bsc4I CCNNNNNNNGG 5 cut(s) 97, 481, 605, 700, 1037
Bse118I RCCGGY 6 cut(s) 68, 230, 326, 349, 553, 1100
Bse1I ACTGG 3 cut(s) 1246, 1541, 1552
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 5 cut(s) 98, 476, 1137, 1409, 1421
BseDI CCNNGG 8 cut(s) 32, 42, 145, 261, 1135, 1145, 1419, 1596
BseGI GGATG 1 cut(s) 1331
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 5 cut(s) 97, 481, 605, 700, 1037
BseMII CTCAG 7 cut(s) 319, 452, 728, 1132, 1169, 1272, 1370
BseNI ACTGG 3 cut(s) 1246, 1541, 1552
BsePI GCGCGC 2 cut(s) 254, 256
BseRI GAGGAG 1 cut(s) 1625
BseSI GKGCMC 2 cut(s) 375, 732
BseXI GCAGC 6 cut(s) 59, 197, 200, 680, 1660, 1726
BseYI CCCAGC 2 cut(s) 64, 1041
Bsh1236I CGCG 5 cut(s) 254, 256, 258, 275, 1011
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 9 cut(s) 37, 90, 373, 730, 752, 761, 930, 1076, 1515
BshNI GGYRCC 3 cut(s) 789, 826, 1361
BshTI ACCGGT 1 cut(s) 1100
BsiEI CGRYCG 1 cut(s) 420
BsiHKAI GWGCWC 4 cut(s) 198, 225, 447, 837
BsiHKCI CYCGRG 1 cut(s) 1728
BslFI GGGAC 5 cut(s) 47, 128, 406, 605, 868
BslI CCNNNNNNNGG 5 cut(s) 97, 481, 605, 700, 1037
BsmAI GTCTC 1 cut(s) 606
BsmFI GGGAC 5 cut(s) 47, 128, 406, 605, 868
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 9 cut(s) 37, 90, 373, 730, 752, 761, 930, 1076, 1515
Bso31I GGTCTC 1 cut(s) 606
BsoBI CYCGRG 1 cut(s) 1728
Bsp120I GGGCCC 2 cut(s) 371, 728
Bsp1286I GDGCHC 7 cut(s) 198, 225, 306, 375, 447, 732, 837
Bsp143I GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 4 cut(s) 91, 106, 240, 1273
BspANI GGCC 9 cut(s) 37, 90, 373, 730, 752, 761, 930, 1076, 1515
BspCNI CTCAG 7 cut(s) 318, 451, 729, 1131, 1168, 1273, 1369
BspFNI CGCG 5 cut(s) 254, 256, 258, 275, 1011
BspHI TCATGA 1 cut(s) 1507
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 4 cut(s) 397, 410, 1324, 1432
BspT107I GGYRCC 3 cut(s) 789, 826, 1361
BspTNI GGTCTC 1 cut(s) 606
BsrFI RCCGGY 6 cut(s) 68, 230, 326, 349, 553, 1100
BsrI ACTGG 3 cut(s) 1246, 1541, 1552
BssAI RCCGGY 6 cut(s) 68, 230, 326, 349, 553, 1100
BssECI CCNNGG 8 cut(s) 32, 42, 145, 261, 1135, 1145, 1419, 1596
BssHII GCGCGC 2 cut(s) 254, 256
BssMI GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 1596
Bst2UI CCWGG 5 cut(s) 98, 476, 1137, 1409, 1421
Bst4CI ACNGT 5 cut(s) 295, 338, 463, 685, 1097
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 686
BstBAI YACGTR 1 cut(s) 1604
BstC8I GCNNGC 8 cut(s) 70, 232, 256, 258, 551, 585, 774, 1387
BstDEI CTNAG 9 cut(s) 305, 438, 737, 1118, 1155, 1172, 1281, 1356, 1764
BstENI CCTNNNNNAGG 1 cut(s) 698
BstF5I GGATG 1 cut(s) 1331
BstFNI CGCG 5 cut(s) 254, 256, 258, 275, 1011
BstHHI GCGC 7 cut(s) 256, 258, 260, 275, 505, 637, 1687
BstKTI GATC 7 cut(s) 120, 183, 405, 420, 975, 1319, 1440
BstMAI GTCTC 1 cut(s) 606
BstMBI GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
BstMCI CGRYCG 1 cut(s) 420
BstNI CCWGG 5 cut(s) 98, 476, 1137, 1409, 1421
BstSCI CCNGG 7 cut(s) 96, 145, 260, 474, 1135, 1407, 1419
BstSFI CTRYAG 1 cut(s) 906
BstSLI GKGCMC 2 cut(s) 375, 732
BstSNI TACGTA 1 cut(s) 1604
BstUI CGCG 5 cut(s) 254, 256, 258, 275, 1011
BstV1I GCAGC 6 cut(s) 59, 197, 200, 680, 1660, 1726
BstX2I RGATCY 2 cut(s) 402, 1316
BstYI RGATCY 2 cut(s) 402, 1316
BsuRI GGCC 9 cut(s) 37, 90, 373, 730, 752, 761, 930, 1076, 1515
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 1331
BtsI GCAGTG 3 cut(s) 486, 711, 1221
BtsIMutI CAGTG 4 cut(s) 343, 486, 711, 1221
Cac8I GCNNGC 8 cut(s) 70, 232, 256, 258, 551, 585, 774, 1387
CciI TCATGA 1 cut(s) 1507
CfoI GCGC 7 cut(s) 256, 258, 260, 275, 505, 637, 1687
Cfr10I RCCGGY 6 cut(s) 68, 230, 326, 349, 553, 1100
CseI GACGC 2 cut(s) 256, 298
Csp6I GTAC 7 cut(s) 790, 876, 961, 1098, 1294, 1601, 1605
CspAI ACCGGT 1 cut(s) 1100
CspCI CAANNNNNGTGG 4 cut(s) 978, 1013, 1462, 1497
CviAII CATG 5 cut(s) 506, 994, 1051, 1251, 1508
CviQI GTAC 7 cut(s) 790, 876, 961, 1098, 1294, 1601, 1605
DdeI CTNAG 9 cut(s) 305, 438, 737, 1118, 1155, 1172, 1281, 1356, 1764
DpnI GATC 7 cut(s) 119, 182, 404, 419, 974, 1318, 1439
DpnII GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
EaeI YGGCCR 1 cut(s) 1074
EciI GGCGGA 1 cut(s) 1262
Ecl136II GAGCTC 3 cut(s) 196, 223, 445
Eco105I TACGTA 1 cut(s) 1604
Eco130I CCWWGG 1 cut(s) 1596
Eco147I AGGCCT 1 cut(s) 761
Eco24I GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Eco31I GGTCTC 1 cut(s) 606
Eco47I GGWCC 5 cut(s) 61, 142, 172, 393, 1028
Eco53kI GAGCTC 3 cut(s) 196, 223, 445
Eco57I CTGAAG 2 cut(s) 1106, 1644
Eco88I CYCGRG 1 cut(s) 1728
EcoICRI GAGCTC 3 cut(s) 196, 223, 445
EcoNI CCTNNNNNAGG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 1298
EcoRII CCWGG 5 cut(s) 96, 474, 1135, 1407, 1419
EcoT14I CCWWGG 1 cut(s) 1596
EcoT38I GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
ErhI CCWWGG 1 cut(s) 1596
FaeI CATG 5 cut(s) 509, 997, 1054, 1254, 1511
FaqI GGGAC 5 cut(s) 47, 128, 406, 605, 868
FatI CATG 5 cut(s) 505, 993, 1050, 1250, 1507
FbaI TGATCA 1 cut(s) 972
Fnu4HI GCNGC 9 cut(s) 73, 91, 107, 211, 214, 240, 669, 1649, 1740
FokI GGATG 1 cut(s) 1338
FriOI GRGCYC 6 cut(s) 198, 225, 306, 375, 447, 732
Fsp4HI GCNGC 9 cut(s) 73, 91, 107, 211, 214, 240, 669, 1649, 1740
FspAI RTGCGCAY 1 cut(s) 504
FspBI CTAG 3 cut(s) 783, 879, 1701
FspI TGCGCA 2 cut(s) 504, 1686
GlaI GCGC 7 cut(s) 255, 257, 259, 274, 504, 636, 1686
GluI GCNGC 9 cut(s) 73, 91, 107, 211, 214, 240, 669, 1649, 1740
GsaI CCCAGC 2 cut(s) 68, 1045
HaeIII GGCC 9 cut(s) 37, 90, 373, 730, 752, 761, 930, 1076, 1515
HgaI GACGC 2 cut(s) 256, 298
HhaI GCGC 7 cut(s) 256, 258, 260, 275, 505, 637, 1687
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 509, 997, 1054, 1254, 1511
Hin6I GCGC 7 cut(s) 254, 256, 258, 273, 503, 635, 1685
HinP1I GCGC 7 cut(s) 254, 256, 258, 273, 503, 635, 1685
HinfI GANTC 5 cut(s) 487, 902, 1140, 1247, 1504
HphI GGTGA 9 cut(s) 364, 464, 496, 587, 1067, 1204, 1256, 1406, 1586
Hpy166II GTNNAC 2 cut(s) 559, 1448
Hpy188I TCNGA 6 cut(s) 402, 1121, 1149, 1158, 1266, 1442
Hpy8I GTNNAC 2 cut(s) 559, 1448
Hpy99I CGWCG 3 cut(s) 248, 272, 368
HpyAV CCTTC 5 cut(s) 18, 535, 751, 973, 1571
HpyCH4III ACNGT 5 cut(s) 295, 338, 463, 685, 1097
HpyCH4IV ACGT 3 cut(s) 132, 1375, 1603
HpyF3I CTNAG 9 cut(s) 305, 438, 737, 1118, 1155, 1172, 1281, 1356, 1764
HpySE526I ACGT 3 cut(s) 132, 1375, 1603
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 509, 997, 1054, 1254, 1511
HspAI GCGC 7 cut(s) 254, 256, 258, 273, 503, 635, 1685
KpnI GGTACC 1 cut(s) 793
KroI GCCGGC 2 cut(s) 68, 230
KroNI GCCGGC 2 cut(s) 70, 232
Ksp22I TGATCA 1 cut(s) 972
Kzo9I GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
LmnI GCTCC 7 cut(s) 52, 193, 442, 450, 832, 1000, 1275
Lsp1109I GCAGC 6 cut(s) 59, 197, 200, 680, 1660, 1726
LweI GCATC 5 cut(s) 490, 769, 901, 1296, 1549
MaeI CTAG 3 cut(s) 783, 879, 1701
MaeII ACGT 3 cut(s) 132, 1375, 1603
MaeIII GTNAC 7 cut(s) 283, 463, 470, 1012, 1210, 1244, 1411
MalI GATC 7 cut(s) 119, 182, 404, 419, 974, 1318, 1439
MauBI CGCGCGCG 1 cut(s) 254
MboI GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
MboII GAAGA 2 cut(s) 509, 1099
MfeI CAATTG 2 cut(s) 1258, 1629
MflI RGATCY 2 cut(s) 402, 1316
MhlI GDGCHC 7 cut(s) 198, 225, 306, 375, 447, 732, 837
MlsI TGGCCA 1 cut(s) 1076
MluNI TGGCCA 1 cut(s) 1076
MlyI GAGTC 3 cut(s) 496, 911, 1241
MmeI TCCRAC 1 cut(s) 642
Mox20I TGGCCA 1 cut(s) 1076
MroNI GCCGGC 2 cut(s) 68, 230
MscI TGGCCA 1 cut(s) 1076
MseI TTAA 4 cut(s) 939, 1191, 1530, 1619
MslI CAYNNNNRTG 2 cut(s) 1572, 1692
Msp20I TGGCCA 1 cut(s) 1076
MspA1I CMGCKG 2 cut(s) 671, 1739
MspR9I CCNGG 7 cut(s) 98, 147, 262, 476, 1137, 1409, 1421
MunI CAATTG 2 cut(s) 1258, 1629
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 5 cut(s) 98, 476, 1137, 1409, 1421
MvnI CGCG 5 cut(s) 254, 256, 258, 275, 1011
NaeI GCCGGC 2 cut(s) 70, 232
NciI CCSGG 2 cut(s) 147, 262
NdeII GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
NgoMIV GCCGGC 2 cut(s) 68, 230
NlaIII CATG 5 cut(s) 509, 997, 1054, 1254, 1511
NmeAIII GCCGAG 5 cut(s) 13, 23, 82, 211, 1326
NmuCI GTSAC 5 cut(s) 283, 470, 1012, 1210, 1244
NsbI TGCGCA 2 cut(s) 504, 1686
OliI CACNNNNGTG 1 cut(s) 1572
PagI TCATGA 1 cut(s) 1507
PauI GCGCGC 2 cut(s) 254, 256
PceI AGGCCT 1 cut(s) 761
PcsI WCGNNNNNNNCGW 3 cut(s) 120, 426, 527
PctI GAATGC 1 cut(s) 689
PdiI GCCGGC 2 cut(s) 70, 232
PfeI GAWTC 2 cut(s) 1140, 1504
PflFI GACNNNGTC 1 cut(s) 617
PflMI CCANNNNNTGG 2 cut(s) 481, 1037
PinAI ACCGGT 1 cut(s) 1100
PkrI GCNGC 9 cut(s) 74, 92, 108, 212, 215, 241, 670, 1650, 1741
Ple19I CGATCG 1 cut(s) 420
PleI GAGTC 3 cut(s) 495, 910, 1241
PpsI GAGTC 3 cut(s) 495, 910, 1241
Ppu21I YACGTR 1 cut(s) 1604
Psp124BI GAGCTC 3 cut(s) 198, 225, 447
Psp6I CCWGG 5 cut(s) 96, 474, 1135, 1407, 1419
PspFI CCCAGC 2 cut(s) 64, 1041
PspGI CCWGG 5 cut(s) 96, 474, 1135, 1407, 1419
PspOMI GGGCCC 2 cut(s) 371, 728
PstI CTGCAG 1 cut(s) 910
PsuI RGATCY 2 cut(s) 402, 1316
PsyI GACNNNGTC 1 cut(s) 617
PteI GCGCGC 2 cut(s) 254, 256
PvuI CGATCG 1 cut(s) 420
PvuII CAGCTG 2 cut(s) 671, 1739
RsaI GTAC 7 cut(s) 791, 877, 962, 1099, 1295, 1602, 1606
RsaNI GTAC 7 cut(s) 790, 876, 961, 1098, 1294, 1601, 1605
RseI CAYNNNNRTG 2 cut(s) 1572, 1692
SacI GAGCTC 3 cut(s) 198, 225, 447
SaqAI TTAA 4 cut(s) 939, 1191, 1530, 1619
SatI GCNGC 9 cut(s) 73, 91, 107, 211, 214, 240, 669, 1649, 1740
Sau3AI GATC 7 cut(s) 117, 180, 402, 417, 972, 1316, 1437
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 3 cut(s) 496, 911, 1241
ScrFI CCNGG 7 cut(s) 98, 147, 262, 476, 1137, 1409, 1421
SduI GDGCHC 7 cut(s) 198, 225, 306, 375, 447, 732, 837
SfaNI GCATC 5 cut(s) 490, 769, 901, 1296, 1549
SfcI CTRYAG 1 cut(s) 906
SfiI GGCCNNNNNGGCC 1 cut(s) 758
SgrAI CRCCGGYG 1 cut(s) 326
SinI GGWCC 5 cut(s) 61, 142, 172, 393, 1028
SmiMI CAYNNNNRTG 2 cut(s) 1572, 1692
SnaBI TACGTA 1 cut(s) 1604
SpeI ACTAGT 1 cut(s) 878
SseBI AGGCCT 1 cut(s) 761
SsiI CCGC 4 cut(s) 91, 106, 240, 1273
SspMI CTAG 3 cut(s) 783, 879, 1701
SstI GAGCTC 3 cut(s) 198, 225, 447
StuI AGGCCT 1 cut(s) 761
StyD4I CCNGG 7 cut(s) 96, 145, 260, 474, 1135, 1407, 1419
StyI CCWWGG 1 cut(s) 1596
TaaI ACNGT 5 cut(s) 295, 338, 463, 685, 1097
TaiI ACGT 3 cut(s) 135, 1378, 1606
TaqI TCGA 5 cut(s) 114, 530, 768, 1494, 1674
TaqII GACCGA 1 cut(s) 130
TatI WGTACW 1 cut(s) 875
TauI GCSGC 3 cut(s) 93, 109, 242
TfiI GAWTC 2 cut(s) 1140, 1504
Tru1I TTAA 4 cut(s) 939, 1191, 1530, 1619
Tru9I TTAA 4 cut(s) 939, 1191, 1530, 1619
TscAI CASTG 4 cut(s) 343, 486, 711, 1228
TseFI GTSAC 5 cut(s) 283, 470, 1012, 1210, 1244
TseI GCWGC 6 cut(s) 72, 210, 213, 668, 1648, 1739
Tsp45I GTSAC 5 cut(s) 283, 470, 1012, 1210, 1244
TspDTI ATGAA 5 cut(s) 838, 1039, 1179, 1496, 1660
TspRI CASTG 4 cut(s) 343, 486, 711, 1228
Tth111I GACNNNGTC 1 cut(s) 617
Van91I CCANNNNNTGG 2 cut(s) 481, 1037
VpaK11BI GGWCC 5 cut(s) 61, 142, 172, 393, 1028
XagI CCTNNNNNAGG 1 cut(s) 698
XapI RAATTY 2 cut(s) 1122, 1298
XcmI CCANNNNNNNNNTGG 3 cut(s) 739, 1038, 1310
XspI CTAG 3 cut(s) 783, 879, 1701
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.