MD03G1065100.v1.1

Pyruvate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001627
Physical Location & Seq
Reverse (-)
5187807 .. 5190668
2862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1065100.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGGCCACCAGCATTGGCTCCCTAGACTCATTAAAACCTGCCAACAACTGCGTCGGTTGCCCTCCCCAAAACAGTGCTACCACCATCTACGACTCCTCCCACCCTCCCTCCGCCATCGCCCCTGCTGACTCCACTCTCGGCCGCCACTTAGCACGCCGCCTGGTGGAGATCGGGGTGGGGGACGTGTTCTCTGTCCCCGGGGATTTTAACCTAACCCTACTAGACCATCTCATTGCTGAACCGGGGCTCACCAACATCGGCTGCTGCAATGAGCTCAATGCCGGGTACGCCGCTGATGGGTACGCCCGGGCCCGTGGCGTGGGGGCGTGCGTTGTCACGTTTACCGTGGGTGGGCTTAGTGTTCTCAATGCCATTGCTGGAGCTTACAGTGAGAACCTTCCTGTTATTTGTGTTGTTGGAGGGCCTAACACAAATGATTATGGCACCAACAGAATTCTTCACCACACCATTGGCTTGCCGGATTTCAGCCAAGAACTCAGATGCTTCCAGACTGTCACTTGCTATCAAGCTGTGGTGAACAACTTGGACGATGCACATGAACAGATTGACACAGCCATTTCTACTGCTCTCAAAGAAAGCAGGCCTGTATACATTAGCATCAGCTGCAACTTGCCTGCCATTCCACATCCAACTTTTAGCAGAGAGCCCATTAAATTTTGCATCTCACCTAGAATGACCAACAAGATGGGGCTAGAAGCCGCTGTGGAGGCGGCAGCTGCCTTCTTGAACAAGGCAGTGAAGCCAGTTGTGGTTGGAGGACCCAAGCTCCGAGTGGCAAAGGCTCATGAAGCCTTTGTTGAATTTGCAGATGCCAGTGGCTATGCTGTTTCTGTGATGCCATCAGCCAAAGGGCATTTCCCCGAAACACATCCTCGCTACATCGGGATATACTGGGGAGCAGTCGGCACCGCCTACTGCGGTGAGATTGTGGAGTCTGCTGATGCATACGTGTTCGCCGGTCCAACCTTCAATGACTACAGCTCGGTTGGCTACTCTCTCCTTCTCAAAAAGGAGAAGGCAATCATTGTGAAGCCCGATAGCGTGGTGGTTGGCAATGGCCCTTCTTTTGGGTGTGTCCTAATGAAGGACTTTCTTCAGGCACTTGCGAAGAGGGTCACCAAAAACACAAGCGCTTACGAAAACTACGCCAGAATCTTCGTCCCAAATGGCCAGCCTCCCAAATGTCAGCCTAATGAGCCTCTGAGAGTGAACATTCTGTTCCAGCACATTCAGAAAATGCTTTCAGCTGACACCGCGGTTATTGCTGAGACCGGTGATTCTTGGTTCAACTGCCTGAAACTGAAACTGCCGGAAGGATGTGGGTATGAGTTCCAGATGCAGTATGGCTCAATCGGGTGGTCTGTTGGTGCAACTCTTGGCTATGCACAGTCTGTTCCAAAGAAGCGCGTCATTGCTTGCATCGGTGATGGGAGCTTCCAGGTGACTGCACAAGATGTGTCAACGATGATCCGATGCAATCAAAGGAGCATCATCTTCCTCATAAACAATGGAGGATACACCATAGAAGTTGAAATCCATGATGGGCCTTACAATGTGATCAAGAACTGGAACTACACCGGTTTGGTGGATGCAATCCATAACGGCGAAGGAAAATGCTGGACAACAAAGGTTCGTACCGAGGAGGAGCTAACGGAAGCGATTGCAACAGCAACTGGGGAGAAGAAAGACTGCTTGTGCTTCATTGAAGTGATTTTTCACAAGGATGACACAAGCAAAGAGCTGCTTGAATGGGGATCAAGGGTCTCTTCTGCCAATAGCCGCCTGCCAAATCCTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

607

Amino Acids

65.31

Weight (kDa)

5.99

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 45 - 150 1.3e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 242 - 361 8.2e-24 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 453 - 577 1.1e-13 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccB1I GGYRCC 2 cut(s) 443, 926
AccI GTMKAC 1 cut(s) 609
AccII CGCG 2 cut(s) 1277, 1428
AclWI GGATC 2 cut(s) 1483, 1783
AcoI YGGCCR 3 cut(s) 3, 139, 1189
AcsI RAATTY 3 cut(s) 453, 674, 821
AcuI CTGAAG 1 cut(s) 1100
AfaI GTAC 3 cut(s) 287, 302, 1657
AfeI AGCGCT 1 cut(s) 1153
AfiI CCNNNNNNNGG 6 cut(s) 163, 297, 319, 351, 1088, 1105
AflIII ACRYGT 2 cut(s) 183, 969
AgeI ACCGGT 2 cut(s) 1292, 1598
AgsI TTSAA 7 cut(s) 748, 821, 991, 1309, 1553, 1727, 1769
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 2 cut(s) 159, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 2 cut(s) 1283, 1789
AlwI GGATC 2 cut(s) 1483, 1783
AlwNI CAGNNNCTG 1 cut(s) 1694
Ama87I CYCGRG 2 cut(s) 197, 306
Aor51HI AGCGCT 1 cut(s) 1153
AoxI GGCC 8 cut(s) 3, 139, 309, 422, 602, 1078, 1189, 1565
ApaI GGGCCC 1 cut(s) 313
ApeKI GCWGC 6 cut(s) 261, 264, 624, 734, 737, 1762
ApoI RAATTY 3 cut(s) 453, 674, 821
AsiGI ACCGGT 2 cut(s) 1292, 1598
AspLEI GCGC 2 cut(s) 1154, 1428
AspS9I GGNCC 7 cut(s) 309, 310, 422, 779, 980, 1079, 1565
AsuC2I CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
AsuHPI GGTGA 9 cut(s) 241, 452, 547, 678, 953, 1129, 1307, 1457, 1474
AvaI CYCGRG 2 cut(s) 197, 306
AvaII GGWCC 2 cut(s) 779, 980
BaeGI GKGCMC 1 cut(s) 313
BalI TGGCCA 2 cut(s) 5, 1191
BanI GGYRCC 2 cut(s) 443, 926
BanII GRGCYC 4 cut(s) 249, 276, 313, 669
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 6 cut(s) 248, 251, 611, 724, 746, 1749
BccI CCATC 8 cut(s) 92, 122, 234, 290, 700, 868, 1442, 1556
BceAI ACGGC 1 cut(s) 1639
BciT130I CCWGG 2 cut(s) 161, 1460
BciVI GTATCC 1 cut(s) 1529
BclI TGATCA 1 cut(s) 1578
BcnI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
BcoDI GTCTC 2 cut(s) 1283, 1789
BfaI CTAG 4 cut(s) 23, 221, 690, 713
BfmI CTRYAG 1 cut(s) 997
BfoI RGCGCY 1 cut(s) 1155
BfuAI ACCTGC 1 cut(s) 46
BfuI GTATCC 1 cut(s) 1529
Bme1390I CCNGG 8 cut(s) 161, 198, 199, 243, 283, 307, 308, 1460
Bme18I GGWCC 2 cut(s) 779, 980
BmeT110I CYCGRG 2 cut(s) 197, 306
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 7 cut(s) 309, 310, 422, 779, 980, 1079, 1565
BmiI GGNNCC 5 cut(s) 19, 311, 445, 781, 928
BmrFI CCNGG 8 cut(s) 161, 198, 199, 243, 283, 307, 308, 1460
BmrI ACTGGG 2 cut(s) 922, 1704
BmuI ACTGGG 2 cut(s) 922, 1704
BpmI CTGGAG 1 cut(s) 399
BpuMI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
BsaAI YACGTR 1 cut(s) 970
BsaI GGTCTC 2 cut(s) 1283, 1789
BsaJI CCNNGG 9 cut(s) 196, 197, 198, 242, 306, 313, 345, 1275, 1659
BsaWI WCCGGW 2 cut(s) 1292, 1598
BsaXI ACNNNNNCTCC 6 cut(s) 80, 92, 110, 122, 771, 801
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 297, 319, 351, 1088, 1105
Bse118I RCCGGY 3 cut(s) 977, 1292, 1598
Bse1I ACTGG 5 cut(s) 764, 834, 917, 1592, 1699
Bse3DI GCAATG 5 cut(s) 231, 274, 372, 1081, 1431
BseBI CCWGG 2 cut(s) 161, 1460
BseDI CCNNGG 9 cut(s) 196, 197, 198, 242, 306, 313, 345, 1275, 1659
BseGI GGATG 5 cut(s) 646, 889, 1343, 1615, 1750
BseLI CCNNNNNNNGG 6 cut(s) 163, 297, 319, 351, 1088, 1105
BseMI GCAATG 5 cut(s) 231, 274, 372, 1081, 1431
BseMII CTCAG 3 cut(s) 511, 1214, 1278
BseNI ACTGG 5 cut(s) 764, 834, 917, 1592, 1699
BseRI GAGGAG 3 cut(s) 85, 1676, 1679
BseSI GKGCMC 1 cut(s) 313
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 6 cut(s) 248, 251, 611, 724, 746, 1749
BsgI GTGCAG 1 cut(s) 1452
Bsh1236I CGCG 2 cut(s) 1277, 1428
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 8 cut(s) 5, 141, 311, 424, 604, 1080, 1191, 1567
BshNI GGYRCC 2 cut(s) 443, 926
BshTI ACCGGT 2 cut(s) 1292, 1598
BsiEI CGRYCG 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 197, 306
BsiSI CCGG 9 cut(s) 198, 242, 282, 307, 479, 978, 1293, 1331, 1599
BslFI GGGAC 3 cut(s) 179, 194, 1166
BslI CCNNNNNNNGG 6 cut(s) 163, 297, 319, 351, 1088, 1105
BsmAI GTCTC 2 cut(s) 1283, 1789
BsmFI GGGAC 3 cut(s) 179, 194, 1166
BsnI GGCC 8 cut(s) 5, 141, 311, 424, 604, 1080, 1191, 1567
Bso31I GGTCTC 2 cut(s) 1283, 1789
BsoBI CYCGRG 2 cut(s) 197, 306
Bsp120I GGGCCC 1 cut(s) 309
Bsp1286I GDGCHC 4 cut(s) 249, 276, 313, 669
Bsp143I GATC 4 cut(s) 168, 1488, 1578, 1775
BspANI GGCC 8 cut(s) 5, 141, 311, 424, 604, 1080, 1191, 1567
BspCNI CTCAG 3 cut(s) 510, 1215, 1279
BspFNI CGCG 2 cut(s) 1277, 1428
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 5 cut(s) 19, 311, 445, 781, 928
BspMI ACCTGC 1 cut(s) 46
BspPI GGATC 2 cut(s) 1483, 1783
BspT107I GGYRCC 2 cut(s) 443, 926
BspTNI GGTCTC 2 cut(s) 1283, 1789
BsrDI GCAATG 5 cut(s) 231, 274, 372, 1081, 1431
BsrFI RCCGGY 3 cut(s) 977, 1292, 1598
BsrI ACTGG 5 cut(s) 764, 834, 917, 1592, 1699
BssAI RCCGGY 3 cut(s) 977, 1292, 1598
BssECI CCNNGG 9 cut(s) 196, 197, 198, 242, 306, 313, 345, 1275, 1659
BssMI GATC 4 cut(s) 168, 1488, 1578, 1775
BssNAI GTATAC 1 cut(s) 610
Bst1107I GTATAC 1 cut(s) 610
Bst2UI CCWGG 2 cut(s) 161, 1460
Bst4CI ACNGT 5 cut(s) 74, 346, 389, 514, 1410
Bst6I CTCTTC 2 cut(s) 1124, 1792
BstAPI GCANNNNNTGC 1 cut(s) 624
BstBAI YACGTR 1 cut(s) 970
BstC8I GCNNGC 8 cut(s) 154, 328, 476, 602, 636, 1193, 1438, 1805
BstDEI CTNAG 5 cut(s) 148, 356, 497, 1223, 1287
BstDSI CCRYGG 3 cut(s) 313, 345, 1275
BstEII GGTNACC 1 cut(s) 1135
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 5 cut(s) 646, 889, 1343, 1615, 1750
BstFNI CGCG 2 cut(s) 1277, 1428
BstH2I RGCGCY 1 cut(s) 1155
BstHHI GCGC 2 cut(s) 1154, 1428
BstKTI GATC 4 cut(s) 171, 1491, 1581, 1778
BstMAI GTCTC 2 cut(s) 1283, 1789
BstMBI GATC 4 cut(s) 168, 1488, 1578, 1775
BstMCI CGRYCG 1 cut(s) 142
BstNI CCWGG 2 cut(s) 161, 1460
BstPI GGTNACC 1 cut(s) 1135
BstSCI CCNGG 8 cut(s) 159, 196, 197, 241, 281, 305, 306, 1458
BstSFI CTRYAG 1 cut(s) 997
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 2 cut(s) 1277, 1428
BstV1I GCAGC 6 cut(s) 248, 251, 611, 724, 746, 1749
BstXI CCANNNNNNTGG 2 cut(s) 470, 706
BstZ17I GTATAC 1 cut(s) 610
BstZI CGGCCG 1 cut(s) 139
BsuI GTATCC 1 cut(s) 1529
BsuRI GGCC 8 cut(s) 5, 141, 311, 424, 604, 1080, 1191, 1567
BtgI CCRYGG 3 cut(s) 313, 345, 1275
BtgZI GCGATG 1 cut(s) 100
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 5 cut(s) 646, 889, 1343, 1615, 1750
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 4 cut(s) 79, 394, 762, 841
BveI ACCTGC 1 cut(s) 46
Cac8I GCNNGC 8 cut(s) 154, 328, 476, 602, 636, 1193, 1438, 1805
CaiI CAGNNNCTG 1 cut(s) 1694
CciI TCATGA 1 cut(s) 805
CfoI GCGC 2 cut(s) 1154, 1428
Cfr10I RCCGGY 3 cut(s) 977, 1292, 1598
Cfr13I GGNCC 7 cut(s) 309, 310, 422, 779, 980, 1079, 1565
Cfr42I CCGCGG 1 cut(s) 1278
Cfr9I CCCGGG 2 cut(s) 197, 306
CseI GACGC 2 cut(s) 40, 1417
Csp6I GTAC 3 cut(s) 286, 301, 1656
CspAI ACCGGT 2 cut(s) 1292, 1598
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 3 cut(s) 557, 806, 1559
CviQI GTAC 3 cut(s) 286, 301, 1656
DdeI CTNAG 5 cut(s) 148, 356, 497, 1223, 1287
DpnI GATC 4 cut(s) 170, 1490, 1580, 1777
DpnII GATC 4 cut(s) 168, 1488, 1578, 1775
EaeI YGGCCR 3 cut(s) 3, 139, 1189
EagI CGGCCG 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 1124, 1792
EarI CTCTTC 2 cut(s) 1124, 1792
EciI GGCGGA 1 cut(s) 100
Ecl136II GAGCTC 1 cut(s) 274
EclXI CGGCCG 1 cut(s) 139
Eco147I AGGCCT 1 cut(s) 604
Eco24I GRGCYC 4 cut(s) 249, 276, 313, 669
Eco31I GGTCTC 2 cut(s) 1283, 1789
Eco47I GGWCC 2 cut(s) 779, 980
Eco47III AGCGCT 1 cut(s) 1153
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 1100
Eco88I CYCGRG 2 cut(s) 197, 306
Eco91I GGTNACC 1 cut(s) 1135
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 2 cut(s) 422, 779
EcoO65I GGTNACC 1 cut(s) 1135
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 2 cut(s) 159, 1458
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 4 cut(s) 249, 276, 313, 669
FaeI CATG 3 cut(s) 560, 809, 1562
FalI AAGNNNNNCTT 4 cut(s) 1749, 1781, 1771, 1803
FaqI GGGAC 3 cut(s) 179, 194, 1166
FatI CATG 3 cut(s) 556, 805, 1558
FbaI TGATCA 1 cut(s) 1578
FblI GTMKAC 1 cut(s) 609
FokI GGATG 5 cut(s) 633, 876, 1350, 1622, 1757
FriOI GRGCYC 4 cut(s) 249, 276, 313, 669
FspBI CTAG 4 cut(s) 23, 221, 690, 713
GlaI GCGC 2 cut(s) 1153, 1427
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 1155
HaeIII GGCC 8 cut(s) 5, 141, 311, 424, 604, 1080, 1191, 1567
HapII CCGG 9 cut(s) 198, 242, 282, 307, 479, 978, 1293, 1331, 1599
HgaI GACGC 2 cut(s) 40, 1417
HhaI GCGC 2 cut(s) 1154, 1428
Hin1II CATG 3 cut(s) 560, 809, 1562
Hin6I GCGC 2 cut(s) 1152, 1426
HinP1I GCGC 2 cut(s) 1152, 1426
HincII GTYRAC 1 cut(s) 1482
HindII GTYRAC 1 cut(s) 1482
HinfI GANTC 6 cut(s) 26, 92, 128, 953, 1173, 1298
HpaII CCGG 9 cut(s) 198, 242, 282, 307, 479, 978, 1293, 1331, 1599
HphI GGTGA 9 cut(s) 241, 452, 547, 678, 953, 1129, 1307, 1457, 1474
Hpy166II GTNNAC 5 cut(s) 342, 538, 610, 1231, 1482
Hpy188I TCNGA 5 cut(s) 500, 791, 1224, 1254, 1493
Hpy188III TCNNGA 6 cut(s) 508, 745, 806, 904, 1354, 1582
Hpy8I GTNNAC 5 cut(s) 342, 538, 610, 1231, 1482
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 9 cut(s) 407, 751, 997, 1030, 1031, 1092, 1099, 1328, 1622
HpyCH4III ACNGT 5 cut(s) 74, 346, 389, 514, 1410
HpyCH4IV ACGT 3 cut(s) 183, 338, 969
HpyF3I CTNAG 5 cut(s) 148, 356, 497, 1223, 1287
HpySE526I ACGT 3 cut(s) 183, 338, 969
Hsp92II CATG 3 cut(s) 560, 809, 1562
HspAI GCGC 2 cut(s) 1152, 1426
Ksp22I TGATCA 1 cut(s) 1578
KspI CCGCGG 1 cut(s) 1278
Kzo9I GATC 4 cut(s) 168, 1488, 1578, 1775
LmnI GCTCC 7 cut(s) 23, 380, 792, 917, 1452, 1506, 1666
Lsp1109I GCAGC 6 cut(s) 248, 251, 611, 724, 746, 1749
MaeI CTAG 4 cut(s) 23, 221, 690, 713
MaeII ACGT 3 cut(s) 183, 338, 969
MaeIII GTNAC 4 cut(s) 334, 514, 1135, 1462
MalI GATC 4 cut(s) 170, 1490, 1580, 1777
MboI GATC 4 cut(s) 168, 1488, 1578, 1775
MboII GAAGA 7 cut(s) 449, 1106, 1141, 1168, 1507, 1714, 1779
MhlI GDGCHC 4 cut(s) 249, 276, 313, 669
MlsI TGGCCA 2 cut(s) 5, 1191
MluCI AATT 3 cut(s) 453, 674, 821
MluNI TGGCCA 2 cut(s) 5, 1191
MlyI GAGTC 4 cut(s) 20, 86, 122, 962
MmeI TCCRAC 4 cut(s) 397, 674, 754, 1007
Mox20I TGGCCA 2 cut(s) 5, 1191
Mph1103I ATGCAT 1 cut(s) 967
MscI TGGCCA 2 cut(s) 5, 1191
MseI TTAA 3 cut(s) 32, 207, 672
MslI CAYNNNNRTG 2 cut(s) 1727, 1743
Msp20I TGGCCA 2 cut(s) 5, 1191
MspA1I CMGCKG 6 cut(s) 293, 624, 722, 737, 1268, 1277
MspI CCGG 9 cut(s) 198, 242, 282, 307, 479, 978, 1293, 1331, 1599
MspR9I CCNGG 8 cut(s) 161, 198, 199, 243, 283, 307, 308, 1460
MvaI CCWGG 2 cut(s) 161, 1460
MvnI CGCG 2 cut(s) 1277, 1428
NciI CCSGG 6 cut(s) 198, 199, 243, 283, 307, 308
NdeII GATC 4 cut(s) 168, 1488, 1578, 1775
NlaIII CATG 3 cut(s) 560, 809, 1562
NlaIV GGNNCC 5 cut(s) 19, 311, 445, 781, 928
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 4 cut(s) 334, 514, 1135, 1462
NsiI ATGCAT 1 cut(s) 967
PagI TCATGA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 604
PfeI GAWTC 2 cut(s) 1173, 1298
PinAI ACCGGT 2 cut(s) 1292, 1598
PleI GAGTC 4 cut(s) 20, 86, 122, 961
PpsI GAGTC 4 cut(s) 20, 86, 122, 961
Ppu21I YACGTR 1 cut(s) 970
PpuMI RGGWCCY 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 779
Psp6I CCWGG 2 cut(s) 159, 1458
PspEI GGTNACC 1 cut(s) 1135
PspGI CCWGG 2 cut(s) 159, 1458
PspN4I GGNNCC 5 cut(s) 19, 311, 445, 781, 928
PspOMI GGGCCC 1 cut(s) 309
PspPI GGNCC 7 cut(s) 309, 310, 422, 779, 980, 1079, 1565
PspPPI RGGWCCY 1 cut(s) 779
PsrI GAACNNNNNNTAC 2 cut(s) 1577, 1609
PstNI CAGNNNCTG 1 cut(s) 1694
PvuII CAGCTG 3 cut(s) 624, 737, 1268
RsaI GTAC 3 cut(s) 287, 302, 1657
RsaNI GTAC 3 cut(s) 286, 301, 1656
RseI CAYNNNNRTG 2 cut(s) 1727, 1743
SacI GAGCTC 1 cut(s) 276
SacII CCGCGG 1 cut(s) 1278
SaqAI TTAA 3 cut(s) 32, 207, 672
Sau3AI GATC 4 cut(s) 168, 1488, 1578, 1775
Sau96I GGNCC 7 cut(s) 309, 310, 422, 779, 980, 1079, 1565
SchI GAGTC 4 cut(s) 20, 86, 122, 962
ScrFI CCNGG 8 cut(s) 161, 198, 199, 243, 283, 307, 308, 1460
SduI GDGCHC 4 cut(s) 249, 276, 313, 669
SfcI CTRYAG 1 cut(s) 997
Sfr303I CCGCGG 1 cut(s) 1278
SgrBI CCGCGG 1 cut(s) 1278
SinI GGWCC 2 cut(s) 779, 980
SmaI CCCGGG 2 cut(s) 199, 308
SmiMI CAYNNNNRTG 2 cut(s) 1727, 1743
SrfI GCCCGGGC 1 cut(s) 308
Sse9I AATT 3 cut(s) 453, 674, 821
SseBI AGGCCT 1 cut(s) 604
SspMI CTAG 4 cut(s) 23, 221, 690, 713
SstI GAGCTC 1 cut(s) 276
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 8 cut(s) 159, 196, 197, 241, 281, 305, 306, 1458
TaaI ACNGT 5 cut(s) 74, 346, 389, 514, 1410
TaiI ACGT 3 cut(s) 186, 341, 972
TasI AATT 3 cut(s) 453, 674, 821
TauI GCSGC 6 cut(s) 144, 159, 293, 722, 734, 1803
TfiI GAWTC 2 cut(s) 1173, 1298
Tru1I TTAA 3 cut(s) 32, 207, 672
Tru9I TTAA 3 cut(s) 32, 207, 672
TscAI CASTG 4 cut(s) 79, 394, 762, 841
TseFI GTSAC 4 cut(s) 334, 514, 1135, 1462
TseI GCWGC 6 cut(s) 261, 264, 624, 734, 737, 1762
Tsp45I GTSAC 4 cut(s) 334, 514, 1135, 1462
TspDTI ATGAA 4 cut(s) 573, 822, 1118, 1711
TspGWI ACGGA 1 cut(s) 1688
TspMI CCCGGG 2 cut(s) 197, 306
TspRI CASTG 4 cut(s) 79, 394, 762, 841
VpaK11BI GGWCC 2 cut(s) 779, 980
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 3 cut(s) 453, 674, 821
XcmI CCANNNNNNNNNTGG 2 cut(s) 790, 1361
XmaI CCCGGG 2 cut(s) 197, 306
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 4 cut(s) 23, 221, 690, 713
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.