RchiOBHm_Chr5g0010191

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001627
Physical Location & Seq
Forward (+)
6765123 .. 6768596
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29098

Sequence Viewer

Length: 1377 bp
ATGGAACCTTCTACACTCATCGGTGCAACGCCTCGGCCCACCTCGGCTCTACTTCCGGTGGTCCCCGCCGCCGCCTGCAGCGGCACGCTGGGCCGCCACCTGGCTCGGCGGCTGGTCGAGATTGGCGTTCATGACGTGTTCTCAGTCCCCGGCGACTTCAACTTGACCCTCTTGGACCACCTGATCGCCGAGCCGGAGCTCAACCTCATCGGCTGCTGCAACGAGCTCAACGCCGGCTACGCCGCCGACGGCTACGCGCGCGCCAGGGGCGTCGGCGCGTGTGTCGTAACTTTCACGGTGGGTGGGCTCAGCGTCTTGAACGCCATCGCCGGTGCTTACAGTGAGAACTTGCCGGTGATTTGTATCGTCGGTGGGCCCAACTCCAATGATTACGGGACCAATCGGATCCTCCACCACACGATCGGGTTACCCGATTTCACTCAGGAGCTCCGGTGCTTCCAAACTGTTACTTGTCACCAGGCAGTGGTGAGTCATTTGGAAGATGCGCATGAGCTTATAGACACGGCAATTTCGACAGCATTGAAGGAGAGCAAGCCGGTTTACATTAGCATAAGTTGTAATTTGCCTGCAATTCCTCACCCCACATTTGGTAGAGACCCTGTTCCCTTCTTTATTGCACCGAAAGTAAGCAATCAAATAGGGTTGGAGGCAGCTGTTGAAGCAACTGCTGCATTCCTGAACAAGGCAGTGAAGCCTAATTACTGCAGAATTTTTGTTCCCCCTGGAATCCCTCTGAAAGCTCAGAAAGATGAACCTCTTAGAGTAAATGTTCTGTTTAAGCACATTCAGGAGATTGTCACCCCAAACACTGCCATAATTTCCGAAACTGGTGACTCATGGTTCAATTGTCAGAAGCTCCGCCTCCCTGAGAATTGTGGGTATGAATTCCAGATGCAATATGGATCTATTGGATGGTCAGTCGGTGCTACTCTCGGTTATGCTCAGGGTGCCAAAGATAAACGTGTAATTGCTTGCATTGGTGATGGGAGTTTCCAGGTGACTGCCCAGGACATTTCAACAATGATCCGATGTGGACAAAATACCATCATATTTCTTATCAACAATGGAGGCTATACAATTGAAGTAGAGATTCATGATGGCCCATACAATGTCATTAAGAACTGGGATTATACTGGTCTTGTTGATGCAATCCACAATGGTGAAGGCAAATGCTGGACTACCAAGGTACGTACAGAGGAGGACTTAACTGAAGCAATTGCAAAAGCAACAGGGGAGCACAAGGATAGTTTATGCTTCATCGAAGTTTTTGTGCACAAAGATGACACTAGCAAAGAGCTTCTGGAATGGGGATCCCGAGTTTCAGCTGCTAACAGCCGACCTCCAAATCCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

458

Amino Acids

49.54

Weight (kDa)

5.53

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 29 - 134 1.2e-29 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_C PF02775 304 - 429 2.7e-15 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 507
AccB1I GGYRCC 1 cut(s) 968
AccB7I CCANNNNNTGG 1 cut(s) 484
AccII CGCG 4 cut(s) 257, 259, 261, 278
AciI CCGC 8 cut(s) 66, 69, 72, 81, 94, 109, 243, 880
AclWI GGATC 6 cut(s) 400, 413, 931, 1039, 1326, 1339
AcsI RAATTY 2 cut(s) 729, 905
AcuI CTGAAG 1 cut(s) 1251
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 2 cut(s) 1209, 1213
AfiI CCNNNNNNNGG 4 cut(s) 100, 484, 608, 703
AflIII ACRYGT 2 cut(s) 135, 982
AgsI TTSAA 7 cut(s) 160, 319, 544, 680, 865, 1038, 1103
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 6 cut(s) 99, 263, 477, 742, 1014, 1026
AleI CACNNNNGTG 1 cut(s) 1179
AluBI AGCT 9 cut(s) 199, 226, 448, 514, 674, 761, 877, 1318, 1346
AluI AGCT 9 cut(s) 199, 226, 448, 514, 674, 761, 877, 1318, 1346
Alw21I GWGCWC 5 cut(s) 201, 228, 450, 1260, 1296
Alw26I GTCTC 1 cut(s) 609
Alw44I GTGCAC 1 cut(s) 1292
AlwI GGATC 6 cut(s) 400, 413, 931, 1039, 1326, 1339
Ama87I CYCGRG 1 cut(s) 1335
AoxI GGCC 4 cut(s) 35, 91, 374, 1120
ApaI GGGCCC 1 cut(s) 378
ApaLI GTGCAC 1 cut(s) 1292
ApeKI GCWGC 6 cut(s) 78, 213, 216, 671, 689, 1346
ApoI RAATTY 2 cut(s) 729, 905
AspLEI GCGC 5 cut(s) 259, 261, 263, 278, 508
AspS9I GGNCC 8 cut(s) 36, 61, 91, 175, 374, 375, 396, 1121
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 9 cut(s) 367, 467, 499, 590, 811, 863, 1013, 1030, 1193
AvaI CYCGRG 1 cut(s) 1335
AvaII GGWCC 3 cut(s) 61, 175, 396
BaeGI GKGCMC 2 cut(s) 378, 1296
BamHI GGATCC 2 cut(s) 405, 1331
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 5 cut(s) 201, 228, 309, 378, 450
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
Bbv12I GWGCWC 5 cut(s) 201, 228, 450, 1260, 1296
BbvI GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1333
BccI CCATC 5 cut(s) 332, 927, 998, 1073, 1112
BceAI ACGGC 2 cut(s) 265, 540
BciT130I CCWGG 6 cut(s) 101, 265, 479, 744, 1016, 1028
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 1 cut(s) 609
BfaI CTAG 1 cut(s) 1308
BfmI CTRYAG 2 cut(s) 76, 724
BlpI GCTNAGC 1 cut(s) 308
Bme1390I CCNGG 7 cut(s) 101, 150, 265, 479, 744, 1016, 1028
Bme18I GGWCC 3 cut(s) 61, 175, 396
BmeT110I CYCGRG 1 cut(s) 1335
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 8 cut(s) 36, 61, 91, 175, 374, 375, 396, 1121
BmiI GGNNCC 7 cut(s) 6, 63, 376, 397, 407, 970, 1333
BmrFI CCNGG 7 cut(s) 101, 150, 265, 479, 744, 1016, 1028
BmrI ACTGGG 1 cut(s) 1153
BmsI GCATC 3 cut(s) 493, 903, 1156
BmuI ACTGGG 1 cut(s) 1153
Bpu10I CCTNAGC 1 cut(s) 963
Bpu1102I GCTNAGC 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 150
BsaAI YACGTR 1 cut(s) 1211
BsaBI GATNNNNATC 1 cut(s) 362
BsaHI GRCGYC 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 609
BsaJI CCNNGG 7 cut(s) 32, 42, 148, 264, 742, 1026, 1203
BsaWI WCCGGW 2 cut(s) 55, 450
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 484, 608, 703
Bse118I RCCGGY 4 cut(s) 233, 329, 352, 556
Bse1I ACTGG 3 cut(s) 853, 1148, 1159
Bse8I GATNNNNATC 1 cut(s) 362
BseBI CCWGG 6 cut(s) 101, 265, 479, 744, 1016, 1028
BseDI CCNNGG 7 cut(s) 32, 42, 148, 264, 742, 1026, 1203
BseGI GGATG 1 cut(s) 938
BseJI GATNNNNATC 1 cut(s) 362
BseLI CCNNNNNNNGG 4 cut(s) 100, 484, 608, 703
BseMII CTCAG 6 cut(s) 156, 322, 455, 776, 879, 977
BseNI ACTGG 3 cut(s) 853, 1148, 1159
BsePI GCGCGC 2 cut(s) 257, 259
BseRI GAGGAG 1 cut(s) 1232
BseSI GKGCMC 2 cut(s) 378, 1296
BseXI GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1333
BseYI CCCAGC 1 cut(s) 88
Bsh1236I CGCG 4 cut(s) 257, 259, 261, 278
Bsh1285I CGRYCG 1 cut(s) 423
BshFI GGCC 4 cut(s) 37, 93, 376, 1122
BshNI GGYRCC 1 cut(s) 968
BsiEI CGRYCG 1 cut(s) 423
BsiHKAI GWGCWC 5 cut(s) 201, 228, 450, 1260, 1296
BsiHKCI CYCGRG 1 cut(s) 1335
BsiSI CCGG 8 cut(s) 56, 150, 194, 234, 330, 353, 451, 557
BslFI GGGAC 3 cut(s) 47, 131, 409
BslI CCNNNNNNNGG 4 cut(s) 100, 484, 608, 703
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 3 cut(s) 47, 131, 409
BsmI GAATGC 1 cut(s) 692
BsnI GGCC 4 cut(s) 37, 93, 376, 1122
Bso31I GGTCTC 1 cut(s) 609
BsoBI CYCGRG 1 cut(s) 1335
Bsp120I GGGCCC 1 cut(s) 374
Bsp1286I GDGCHC 7 cut(s) 201, 228, 309, 378, 450, 1260, 1296
Bsp143I GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
Bsp1720I GCTNAGC 1 cut(s) 308
BspACI CCGC 8 cut(s) 66, 69, 72, 81, 94, 109, 243, 880
BspANI GGCC 4 cut(s) 37, 93, 376, 1122
BspCNI CTCAG 6 cut(s) 155, 321, 454, 775, 880, 976
BspFNI CGCG 4 cut(s) 257, 259, 261, 278
BspHI TCATGA 2 cut(s) 130, 1114
BspLI GGNNCC 7 cut(s) 6, 63, 376, 397, 407, 970, 1333
BspMAI CTGCAG 2 cut(s) 80, 728
BspPI GGATC 6 cut(s) 400, 413, 931, 1039, 1326, 1339
BspT107I GGYRCC 1 cut(s) 968
BspTNI GGTCTC 1 cut(s) 609
BsrFI RCCGGY 4 cut(s) 233, 329, 352, 556
BsrI ACTGG 3 cut(s) 853, 1148, 1159
BssAI RCCGGY 4 cut(s) 233, 329, 352, 556
BssECI CCNNGG 7 cut(s) 32, 42, 148, 264, 742, 1026, 1203
BssHII GCGCGC 2 cut(s) 257, 259
BssMI GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 1203
Bst2UI CCWGG 6 cut(s) 101, 265, 479, 744, 1016, 1028
Bst4CI ACNGT 3 cut(s) 298, 341, 466
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 1211
BstC8I GCNNGC 8 cut(s) 76, 86, 235, 259, 261, 554, 588, 994
BstDEI CTNAG 8 cut(s) 142, 308, 441, 762, 779, 888, 963, 1371
BstEII GGTNACC 1 cut(s) 426
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 1 cut(s) 938
BstFNI CGCG 4 cut(s) 257, 259, 261, 278
BstHHI GCGC 5 cut(s) 259, 261, 263, 278, 508
BstKTI GATC 6 cut(s) 186, 408, 423, 926, 1047, 1334
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
BstMCI CGRYCG 1 cut(s) 423
BstMWI GCNNNNNNNGC 7 cut(s) 90, 239, 258, 267, 680, 689, 968
BstNI CCWGG 6 cut(s) 101, 265, 479, 744, 1016, 1028
BstPI GGTNACC 1 cut(s) 426
BstSCI CCNGG 7 cut(s) 99, 148, 263, 477, 742, 1014, 1026
BstSFI CTRYAG 2 cut(s) 76, 724
BstSLI GKGCMC 2 cut(s) 378, 1296
BstSNI TACGTA 1 cut(s) 1211
BstUI CGCG 4 cut(s) 257, 259, 261, 278
BstV1I GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1333
BstX2I RGATCY 3 cut(s) 405, 923, 1331
BstYI RGATCY 3 cut(s) 405, 923, 1331
BsuRI GGCC 4 cut(s) 37, 93, 376, 1122
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 938
BtsI GCAGTG 3 cut(s) 489, 714, 828
BtsIMutI CAGTG 4 cut(s) 346, 489, 714, 828
Cac8I GCNNGC 8 cut(s) 76, 86, 235, 259, 261, 554, 588, 994
CciI TCATGA 2 cut(s) 130, 1114
CfoI GCGC 5 cut(s) 259, 261, 263, 278, 508
Cfr10I RCCGGY 4 cut(s) 233, 329, 352, 556
Cfr13I GGNCC 8 cut(s) 36, 61, 91, 175, 374, 375, 396, 1121
CseI GACGC 2 cut(s) 259, 301
Csp6I GTAC 2 cut(s) 1208, 1212
CviAII CATG 4 cut(s) 131, 509, 858, 1115
CviQI GTAC 2 cut(s) 1208, 1212
DdeI CTNAG 8 cut(s) 142, 308, 441, 762, 779, 888, 963, 1371
DpnI GATC 6 cut(s) 185, 407, 422, 925, 1046, 1333
DpnII GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
EciI GGCGGA 1 cut(s) 869
Ecl136II GAGCTC 3 cut(s) 199, 226, 448
Eco105I TACGTA 1 cut(s) 1211
Eco130I CCWWGG 1 cut(s) 1203
Eco24I GRGCYC 5 cut(s) 201, 228, 309, 378, 450
Eco31I GGTCTC 1 cut(s) 609
Eco47I GGWCC 3 cut(s) 61, 175, 396
Eco53kI GAGCTC 3 cut(s) 199, 226, 448
Eco57I CTGAAG 1 cut(s) 1251
Eco88I CYCGRG 1 cut(s) 1335
Eco91I GGTNACC 1 cut(s) 426
EcoICRI GAGCTC 3 cut(s) 199, 226, 448
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 426
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 6 cut(s) 99, 263, 477, 742, 1014, 1026
EcoT14I CCWWGG 1 cut(s) 1203
EcoT38I GRGCYC 5 cut(s) 201, 228, 309, 378, 450
ErhI CCWWGG 1 cut(s) 1203
FaeI CATG 4 cut(s) 134, 512, 861, 1118
FaqI GGGAC 3 cut(s) 47, 131, 409
FatI CATG 4 cut(s) 130, 508, 857, 1114
FauI CCCGC 1 cut(s) 73
FokI GGATG 1 cut(s) 945
FriOI GRGCYC 5 cut(s) 201, 228, 309, 378, 450
FspAI RTGCGCAY 1 cut(s) 507
FspBI CTAG 1 cut(s) 1308
FspI TGCGCA 1 cut(s) 507
GlaI GCGC 5 cut(s) 258, 260, 262, 277, 507
GsaI CCCAGC 1 cut(s) 92
HaeIII GGCC 4 cut(s) 37, 93, 376, 1122
HapII CCGG 8 cut(s) 56, 150, 194, 234, 330, 353, 451, 557
HgaI GACGC 2 cut(s) 259, 301
HhaI GCGC 5 cut(s) 259, 261, 263, 278, 508
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 4 cut(s) 134, 512, 861, 1118
Hin6I GCGC 5 cut(s) 257, 259, 261, 276, 506
HinP1I GCGC 5 cut(s) 257, 259, 261, 276, 506
HinfI GANTC 4 cut(s) 490, 747, 854, 1111
HpaII CCGG 8 cut(s) 56, 150, 194, 234, 330, 353, 451, 557
HphI GGTGA 9 cut(s) 367, 467, 499, 590, 811, 863, 1013, 1030, 1193
Hpy166II GTNNAC 3 cut(s) 562, 1055, 1294
Hpy188I TCNGA 6 cut(s) 405, 756, 765, 844, 873, 1049
Hpy8I GTNNAC 3 cut(s) 562, 1055, 1294
Hpy99I CGWCG 3 cut(s) 251, 275, 371
HpyAV CCTTC 4 cut(s) 18, 538, 637, 1178
HpyCH4III ACNGT 3 cut(s) 298, 341, 466
HpyCH4IV ACGT 3 cut(s) 135, 982, 1210
HpyF10VI GCNNNNNNNGC 7 cut(s) 90, 239, 258, 267, 680, 689, 968
HpyF3I CTNAG 8 cut(s) 142, 308, 441, 762, 779, 888, 963, 1371
HpySE526I ACGT 3 cut(s) 135, 982, 1210
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 4 cut(s) 134, 512, 861, 1118
HspAI GCGC 5 cut(s) 257, 259, 261, 276, 506
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Kzo9I GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
LmnI GCTCC 5 cut(s) 196, 445, 453, 882, 1255
Lsp1109I GCAGC 6 cut(s) 90, 200, 203, 676, 683, 1333
LweI GCATC 3 cut(s) 493, 903, 1156
MaeI CTAG 1 cut(s) 1308
MaeII ACGT 3 cut(s) 135, 982, 1210
MaeIII GTNAC 7 cut(s) 286, 426, 466, 473, 817, 851, 1018
MalI GATC 6 cut(s) 185, 407, 422, 925, 1046, 1333
MauBI CGCGCGCG 1 cut(s) 257
MboI GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
MboII GAAGA 1 cut(s) 512
MfeI CAATTG 3 cut(s) 865, 1098, 1236
MflI RGATCY 3 cut(s) 405, 923, 1331
MhlI GDGCHC 7 cut(s) 201, 228, 309, 378, 450, 1260, 1296
MlyI GAGTC 2 cut(s) 499, 848
MmeI TCCRAC 1 cut(s) 645
MroNI GCCGGC 1 cut(s) 233
MseI TTAA 3 cut(s) 798, 1137, 1226
MslI CAYNNNNRTG 2 cut(s) 1179, 1299
MspA1I CMGCKG 3 cut(s) 81, 674, 1346
MspI CCGG 8 cut(s) 56, 150, 194, 234, 330, 353, 451, 557
MspR9I CCNGG 7 cut(s) 101, 150, 265, 479, 744, 1016, 1028
MunI CAATTG 3 cut(s) 865, 1098, 1236
Mva1269I GAATGC 1 cut(s) 692
MvaI CCWGG 6 cut(s) 101, 265, 479, 744, 1016, 1028
MvnI CGCG 4 cut(s) 257, 259, 261, 278
MwoI GCNNNNNNNGC 7 cut(s) 90, 239, 258, 267, 680, 689, 968
NaeI GCCGGC 1 cut(s) 235
NciI CCSGG 1 cut(s) 150
NdeII GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 4 cut(s) 134, 512, 861, 1118
NlaIV GGNNCC 7 cut(s) 6, 63, 376, 397, 407, 970, 1333
NmeAIII GCCGAG 4 cut(s) 13, 23, 85, 214
NmuCI GTSAC 4 cut(s) 473, 817, 851, 1018
NsbI TGCGCA 1 cut(s) 507
OliI CACNNNNGTG 1 cut(s) 1179
PagI TCATGA 2 cut(s) 130, 1114
PauI GCGCGC 2 cut(s) 257, 259
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 429, 530
PctI GAATGC 1 cut(s) 692
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 747, 1111
PflMI CCANNNNNTGG 1 cut(s) 484
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 2 cut(s) 498, 848
PpsI GAGTC 2 cut(s) 498, 848
Ppu21I YACGTR 1 cut(s) 1211
Psp124BI GAGCTC 3 cut(s) 201, 228, 450
Psp6I CCWGG 6 cut(s) 99, 263, 477, 742, 1014, 1026
PspEI GGTNACC 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 6 cut(s) 99, 263, 477, 742, 1014, 1026
PspN4I GGNNCC 7 cut(s) 6, 63, 376, 397, 407, 970, 1333
PspOMI GGGCCC 1 cut(s) 374
PspPI GGNCC 8 cut(s) 36, 61, 91, 175, 374, 375, 396, 1121
PstI CTGCAG 2 cut(s) 80, 728
PsuI RGATCY 3 cut(s) 405, 923, 1331
PteI GCGCGC 2 cut(s) 257, 259
PvuI CGATCG 1 cut(s) 423
PvuII CAGCTG 2 cut(s) 674, 1346
RsaI GTAC 2 cut(s) 1209, 1213
RsaNI GTAC 2 cut(s) 1208, 1212
RseI CAYNNNNRTG 2 cut(s) 1179, 1299
SacI GAGCTC 3 cut(s) 201, 228, 450
SaqAI TTAA 3 cut(s) 798, 1137, 1226
Sau3AI GATC 6 cut(s) 183, 405, 420, 923, 1044, 1331
Sau96I GGNCC 8 cut(s) 36, 61, 91, 175, 374, 375, 396, 1121
SchI GAGTC 2 cut(s) 499, 848
ScrFI CCNGG 7 cut(s) 101, 150, 265, 479, 744, 1016, 1028
SduI GDGCHC 7 cut(s) 201, 228, 309, 378, 450, 1260, 1296
SfaNI GCATC 3 cut(s) 493, 903, 1156
SfcI CTRYAG 2 cut(s) 76, 724
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 3 cut(s) 61, 175, 396
SmiMI CAYNNNNRTG 2 cut(s) 1179, 1299
SnaBI TACGTA 1 cut(s) 1211
SsiI CCGC 8 cut(s) 66, 69, 72, 81, 94, 109, 243, 880
SspMI CTAG 1 cut(s) 1308
SstI GAGCTC 3 cut(s) 201, 228, 450
StyD4I CCNGG 7 cut(s) 99, 148, 263, 477, 742, 1014, 1026
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 3 cut(s) 298, 341, 466
TaiI ACGT 3 cut(s) 138, 985, 1213
TaqI TCGA 3 cut(s) 117, 533, 1281
TauI GCSGC 6 cut(s) 71, 74, 84, 96, 112, 245
TfiI GAWTC 2 cut(s) 747, 1111
Tru1I TTAA 3 cut(s) 798, 1137, 1226
Tru9I TTAA 3 cut(s) 798, 1137, 1226
TscAI CASTG 4 cut(s) 346, 489, 714, 835
TseFI GTSAC 4 cut(s) 473, 817, 851, 1018
TseI GCWGC 6 cut(s) 78, 213, 216, 671, 689, 1346
Tsp45I GTSAC 4 cut(s) 473, 817, 851, 1018
TspDTI ATGAA 5 cut(s) 119, 786, 918, 1103, 1267
TspRI CASTG 4 cut(s) 346, 489, 714, 835
Van91I CCANNNNNTGG 1 cut(s) 484
VneI GTGCAC 1 cut(s) 1292
VpaK11BI GGWCC 3 cut(s) 61, 175, 396
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 2 cut(s) 729, 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 917
XspI CTAG 1 cut(s) 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.