MD11G1069100.v1.1

Pyruvate decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001627
Physical Location & Seq
Reverse (-)
5929741 .. 5932737
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1069100.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGGCCACCAATATTGGCTCCCTAGACTCATCAAAACCGGCCAACAACAACGTCGGCTGCCCTCCTCAAAATGGTACTGCCACCATCCACGACTCCTCCCACCCTCCGTCCACCATTGCCTCCGCTGACTCCACTCTTGGCCGCCACTTAGCGCGCCGCCTGGTGGAGATCGGGGTGGGGGACGTGTTCACCGTCCCCGGGGATTTTAACTTAACCCTATTAGACCACCTCATTGCTGAGCCGAAGCTCACCAACATCGGCTGCTGCAATGAGCTCAATGCCGGGTATGCCGCTGATGGATATGCCCGGGAGCGTGGCGTTGGGGCGTGCGTTGTCACGTTTACCGTGGGTGGGCTTAGTGTTCTCAACGCCATTGCTGGAGCTTACAGTGAGAACCTTCCTGTTATTTGTGTTGTTGGAGGGCCAAACACAAATGATTATGGCACCAACAGAATTCTTCACCACACCATTGGCCTGCCGGATTTCAGCCAAGAACTCAGATGCTTCCAGACTGTCACTTGCTATCAAGCTGTGGTAAACAACTTGGATGATGCACATGAACAAATTGATACTGCCATTTCTACTGCTCTCAAAGAAAGCAAGCCTGTCTATATCAGCATTAGCTGCAACTTGCCTGGCATTCCACATCCAGCTTTTAGCACACACCCCATTAAATTTTCCATCTCACCTAGAATGACCAACAAGATGGGGCTAGAAGCCGCTGTGGAGGCGGCAGCTGCCTTCTTGAACAAGGCAGTGAAGCCAGTTGTGGTTGGCGGACCAAAGATCCGAGTGGCAAAGGCACATGAAGCCTTTGTTGAATTTGCTGATGCCAGTGGCTATGCTGTTGCCATGATGCCATCAGCCAAAGGGCGTTTCCCCGAAACCCATCGTCGCTACATCGGGACATACTGGGGAGCAGTCGGCACGGCCTACTGTGGTGAGATTGTAGAGTCTGCTGATGCATACTTGTTTGCTGGTCCAATCTTCAACGACTACAGCTCTGTTGGATACTCTCTTCTTCTCAAGAGGGAGAAGGCAATTATTGTGCAGCCTGATCGTGTGGTGGTTGGCAATGGCCCTTCTTTCGGGTGTGTCCTAATGAAGGACTTTCTTCAGGCACTTGCACAGCGTGTCACCAAAAACACAACCGCTCACGAAAACTATGCCAGAATCTTCGTCCCAGATGGCCAGCCTCTCAAATCTCAGCCTAATGAGCCTCTTAGAGTGAACATTCTGTTCCAGCACATTCAGAAAATGCTCTCAGCTGACACTGCGGTTATTGCTGAGACCGGTGATTCTTGGTTTAACTGCTTGAAATTGAAACTGCCGGAAGGATGTGGGTATGAATTCCAGATGCAATATGGATCGATTGGTTGGTCTGTTGGTGCAACTCTAGGCTATGCACAGTCAGTTCCGAAGAAGCGCGTCATTGCTTGCATCGGTGATGGGAGCTTCCAGGTGACTGCACAAGATGTGTCAACAATGATTCGATGCAATCAAAAGAGCATCATCTTCCTCATAAACAATGGCGGATACACCATAGAAGTTGAAATCCATGACGGCCCTTACAATGTGATCAAGAACTGGAACTACACTGGTTTGGTGGACGCAATCCACAACGGCGAAGGAAACTGCTGGACAACAAAGGTTCGTACTGAGGAGGAGCTAACGGAGGCAATTGCAACAGCAACGGAGGAGAAGAAAGACTGCTTGTGCTTCATAGAAGTGATTGCTCACAAGGATGACACAAGCAAAGAGCTGCTTGAATGGGGGTCAAGGGTCTCAGCTGCTAATAGCCGCCCGCCAAATCCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

607

Amino Acids

65.41

Weight (kDa)

5.91

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 45 - 150 6e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 242 - 361 7.8e-23 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 453 - 576 4.3e-14 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccBSI CCGCTC 1 cut(s) 1154
AccII CGCG 2 cut(s) 154, 1428
AclWI GGATC 2 cut(s) 781, 1375
AcoI YGGCCR 4 cut(s) 3, 39, 139, 1189
AcsI RAATTY 4 cut(s) 453, 674, 821, 1349
AcuI CTGAAG 1 cut(s) 1100
AdeI CACNNNGTG 1 cut(s) 1133
AfaI GTAC 2 cut(s) 76, 1657
AfiI CCNNNNNNNGG 6 cut(s) 71, 163, 198, 351, 1088, 1105
AflIII ACRYGT 1 cut(s) 183
AgeI ACCGGT 1 cut(s) 1292
AgsI TTSAA 7 cut(s) 748, 821, 991, 1318, 1324, 1553, 1769
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 3 cut(s) 159, 634, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
Alw21I GWGCWC 1 cut(s) 276
Alw26I GTCTC 2 cut(s) 1283, 1789
AlwI GGATC 2 cut(s) 781, 1375
Ama87I CYCGRG 2 cut(s) 197, 306
AoxI GGCC 9 cut(s) 3, 39, 139, 422, 472, 930, 1078, 1189, 1564
ApeKI GCWGC 9 cut(s) 57, 261, 264, 624, 734, 737, 1051, 1762, 1790
ApoI RAATTY 4 cut(s) 453, 674, 821, 1349
AsiGI ACCGGT 1 cut(s) 1292
AspLEI GCGC 3 cut(s) 154, 156, 1428
AspS9I GGNCC 5 cut(s) 422, 779, 980, 1079, 1565
AsuC2I CCSGG 5 cut(s) 198, 199, 283, 307, 308
AsuHPI GGTGA 9 cut(s) 181, 241, 452, 678, 953, 1129, 1307, 1457, 1474
AvaI CYCGRG 2 cut(s) 197, 306
AvaII GGWCC 2 cut(s) 779, 980
BalI TGGCCA 2 cut(s) 5, 1191
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 9 cut(s) 44, 248, 251, 611, 724, 746, 1063, 1749, 1777
BccI CCATC 8 cut(s) 92, 290, 689, 700, 868, 897, 1181, 1442
BceAI ACGGC 3 cut(s) 945, 1579, 1639
BcgI CGANNNNNNTGC 4 cut(s) 1040, 1074, 1148, 1182
BciT130I CCWGG 3 cut(s) 161, 636, 1460
BciVI GTATCC 2 cut(s) 1004, 1529
BclI TGATCA 1 cut(s) 1578
BcnI CCSGG 5 cut(s) 198, 199, 283, 307, 308
BcoDI GTCTC 2 cut(s) 1283, 1789
BfaI CTAG 4 cut(s) 23, 690, 713, 1397
BfmI CTRYAG 1 cut(s) 997
BfuI GTATCC 2 cut(s) 1004, 1529
BlpI GCTNAGC 1 cut(s) 237
Bme1390I CCNGG 8 cut(s) 161, 198, 199, 283, 307, 308, 636, 1460
Bme18I GGWCC 2 cut(s) 779, 980
BmeT110I CYCGRG 2 cut(s) 197, 306
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 5 cut(s) 422, 779, 980, 1079, 1565
BmiI GGNNCC 2 cut(s) 19, 445
BmrFI CCNGG 8 cut(s) 161, 198, 199, 283, 307, 308, 636, 1460
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 9 cut(s) 491, 541, 820, 846, 952, 1347, 1449, 1484, 1518
BmuI ACTGGG 1 cut(s) 922
BpmI CTGGAG 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 1010
BpuMI CCSGG 5 cut(s) 198, 199, 283, 307, 308
Bsa29I ATCGAT 1 cut(s) 1370
BsaI GGTCTC 2 cut(s) 1283, 1789
BsaJI CCNNGG 5 cut(s) 196, 197, 198, 306, 345
BsaWI WCCGGW 1 cut(s) 1292
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 163, 198, 351, 1088, 1105
Bse118I RCCGGY 2 cut(s) 37, 1292
Bse1I ACTGG 5 cut(s) 764, 834, 917, 1592, 1603
Bse3DI GCAATG 6 cut(s) 114, 231, 274, 372, 1081, 1431
BseBI CCWGG 3 cut(s) 161, 636, 1460
BseCI ATCGAT 1 cut(s) 1370
BseDI CCNNGG 5 cut(s) 196, 197, 198, 306, 345
BseGI GGATG 5 cut(s) 84, 553, 646, 1343, 1750
BseLI CCNNNNNNNGG 6 cut(s) 71, 163, 198, 351, 1088, 1105
BseMI GCAATG 6 cut(s) 114, 231, 274, 372, 1081, 1431
BseMII CTCAG 7 cut(s) 228, 511, 1220, 1278, 1278, 1650, 1800
BseNI ACTGG 5 cut(s) 764, 834, 917, 1592, 1603
BsePI GCGCGC 1 cut(s) 152
BseRI GAGGAG 5 cut(s) 54, 85, 1676, 1679, 1712
BseXI GCAGC 9 cut(s) 44, 248, 251, 611, 724, 746, 1063, 1749, 1777
BsgI GTGCAG 2 cut(s) 1070, 1452
Bsh1236I CGCG 2 cut(s) 154, 1428
BshFI GGCC 9 cut(s) 5, 41, 141, 424, 474, 932, 1080, 1191, 1566
BshNI GGYRCC 1 cut(s) 443
BshTI ACCGGT 1 cut(s) 1292
BshVI ATCGAT 1 cut(s) 1370
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 2 cut(s) 197, 306
BsiSI CCGG 7 cut(s) 38, 198, 282, 307, 479, 1293, 1331
BslFI GGGAC 4 cut(s) 179, 194, 919, 1166
BslI CCNNNNNNNGG 6 cut(s) 71, 163, 198, 351, 1088, 1105
BsmAI GTCTC 2 cut(s) 1283, 1789
BsmFI GGGAC 4 cut(s) 179, 194, 919, 1166
BsmI GAATGC 1 cut(s) 639
BsnI GGCC 9 cut(s) 5, 41, 141, 424, 474, 932, 1080, 1191, 1566
Bso31I GGTCTC 2 cut(s) 1283, 1789
BsoBI CYCGRG 2 cut(s) 197, 306
Bsp1286I GDGCHC 1 cut(s) 276
Bsp143I GATC 5 cut(s) 168, 786, 1057, 1367, 1578
Bsp1720I GCTNAGC 1 cut(s) 237
BspANI GGCC 9 cut(s) 5, 41, 141, 424, 474, 932, 1080, 1191, 1566
BspCNI CTCAG 7 cut(s) 229, 510, 1219, 1277, 1279, 1651, 1799
BspDI ATCGAT 1 cut(s) 1370
BspFNI CGCG 2 cut(s) 154, 1428
BspLI GGNNCC 2 cut(s) 19, 445
BspPI GGATC 2 cut(s) 781, 1375
BspT107I GGYRCC 1 cut(s) 443
BspTNI GGTCTC 2 cut(s) 1283, 1789
BsrBI CCGCTC 1 cut(s) 1154
BsrDI GCAATG 6 cut(s) 114, 231, 274, 372, 1081, 1431
BsrFI RCCGGY 2 cut(s) 37, 1292
BsrI ACTGG 5 cut(s) 764, 834, 917, 1592, 1603
BssAI RCCGGY 2 cut(s) 37, 1292
BssECI CCNNGG 5 cut(s) 196, 197, 198, 306, 345
BssHII GCGCGC 1 cut(s) 152
BssMI GATC 5 cut(s) 168, 786, 1057, 1367, 1578
Bst2UI CCWGG 3 cut(s) 161, 636, 1460
Bst4CI ACNGT 6 cut(s) 193, 346, 389, 514, 938, 1410
Bst6I CTCTTC 1 cut(s) 1023
BstAPI GCANNNNNTGC 1 cut(s) 624
BstC8I GCNNGC 7 cut(s) 154, 328, 476, 602, 1193, 1438, 1805
BstDSI CCRYGG 1 cut(s) 345
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 5 cut(s) 84, 553, 646, 1343, 1750
BstFNI CGCG 2 cut(s) 154, 1428
BstHHI GCGC 3 cut(s) 154, 156, 1428
BstKTI GATC 5 cut(s) 171, 789, 1060, 1370, 1581
BstMAI GTCTC 2 cut(s) 1283, 1789
BstMBI GATC 5 cut(s) 168, 786, 1057, 1367, 1578
BstMWI GCNNNNNNNGC 8 cut(s) 287, 624, 728, 737, 809, 1216, 1274, 1283
BstNI CCWGG 3 cut(s) 161, 636, 1460
BstSCI CCNGG 8 cut(s) 159, 196, 197, 281, 305, 306, 634, 1458
BstSFI CTRYAG 1 cut(s) 997
BstUI CGCG 2 cut(s) 154, 1428
BstV1I GCAGC 9 cut(s) 44, 248, 251, 611, 724, 746, 1063, 1749, 1777
BstX2I RGATCY 1 cut(s) 786
BstXI CCANNNNNNTGG 2 cut(s) 470, 706
BstYI RGATCY 1 cut(s) 786
Bsu15I ATCGAT 1 cut(s) 1370
BsuI GTATCC 2 cut(s) 1004, 1529
BsuRI GGCC 9 cut(s) 5, 41, 141, 424, 474, 932, 1080, 1191, 1566
BsuTUI ATCGAT 1 cut(s) 1370
BtgI CCRYGG 1 cut(s) 345
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 5 cut(s) 84, 553, 646, 1343, 1750
BtsI GCAGTG 2 cut(s) 762, 1272
BtsIMutI CAGTG 5 cut(s) 394, 762, 841, 1272, 1596
Cac8I GCNNGC 7 cut(s) 154, 328, 476, 602, 1193, 1438, 1805
CfoI GCGC 3 cut(s) 154, 156, 1428
Cfr10I RCCGGY 2 cut(s) 37, 1292
Cfr13I GGNCC 5 cut(s) 422, 779, 980, 1079, 1565
Cfr9I CCCGGG 2 cut(s) 197, 306
ClaI ATCGAT 1 cut(s) 1370
CseI GACGC 2 cut(s) 1417, 1619
Csp6I GTAC 2 cut(s) 75, 1656
CspAI ACCGGT 1 cut(s) 1292
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 4 cut(s) 557, 806, 853, 1559
CviQI GTAC 2 cut(s) 75, 1656
DpnI GATC 5 cut(s) 170, 788, 1059, 1369, 1580
DpnII GATC 5 cut(s) 168, 786, 1057, 1367, 1578
DraIII CACNNNGTG 1 cut(s) 1133
EaeI YGGCCR 4 cut(s) 3, 39, 139, 1189
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
EciI GGCGGA 2 cut(s) 792, 1548
Ecl136II GAGCTC 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 2 cut(s) 1283, 1789
Eco47I GGWCC 2 cut(s) 779, 980
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 1100
Eco88I CYCGRG 2 cut(s) 197, 306
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoRI GAATTC 2 cut(s) 453, 1349
EcoRII CCWGG 3 cut(s) 159, 634, 1458
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 4 cut(s) 560, 809, 856, 1562
FalI AAGNNNNNCTT 2 cut(s) 1749, 1781
FaqI GGGAC 4 cut(s) 179, 194, 919, 1166
FatI CATG 4 cut(s) 556, 805, 852, 1558
FauI CCCGC 1 cut(s) 1812
FbaI TGATCA 1 cut(s) 1578
FokI GGATG 5 cut(s) 71, 560, 633, 1350, 1757
FriOI GRGCYC 1 cut(s) 276
FspBI CTAG 4 cut(s) 23, 690, 713, 1397
GlaI GCGC 3 cut(s) 153, 155, 1427
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 9 cut(s) 5, 41, 141, 424, 474, 932, 1080, 1191, 1566
HapII CCGG 7 cut(s) 38, 198, 282, 307, 479, 1293, 1331
HgaI GACGC 2 cut(s) 1417, 1619
HhaI GCGC 3 cut(s) 154, 156, 1428
Hin1II CATG 4 cut(s) 560, 809, 856, 1562
Hin6I GCGC 3 cut(s) 152, 154, 1426
HinP1I GCGC 3 cut(s) 152, 154, 1426
HincII GTYRAC 1 cut(s) 1482
HindII GTYRAC 1 cut(s) 1482
HinfI GANTC 7 cut(s) 26, 92, 128, 953, 1173, 1298, 1489
HpaII CCGG 7 cut(s) 38, 198, 282, 307, 479, 1293, 1331
HphI GGTGA 9 cut(s) 181, 241, 452, 678, 953, 1129, 1307, 1457, 1474
Hpy166II GTNNAC 7 cut(s) 111, 189, 342, 538, 1231, 1482, 1609
Hpy188I TCNGA 4 cut(s) 500, 791, 1254, 1419
Hpy188III TCNNGA 7 cut(s) 508, 745, 904, 1027, 1157, 1354, 1582
Hpy8I GTNNAC 7 cut(s) 111, 189, 342, 538, 1231, 1482, 1609
Hpy99I CGWCG 2 cut(s) 56, 897
HpyAV CCTTC 7 cut(s) 407, 751, 1030, 1092, 1099, 1328, 1622
HpyCH4III ACNGT 6 cut(s) 193, 346, 389, 514, 938, 1410
HpyCH4IV ACGT 3 cut(s) 51, 183, 338
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 624, 728, 737, 809, 1216, 1274, 1283
HpySE526I ACGT 3 cut(s) 51, 183, 338
Hsp92II CATG 4 cut(s) 560, 809, 856, 1562
HspAI GCGC 3 cut(s) 152, 154, 1426
Ksp22I TGATCA 1 cut(s) 1578
Kzo9I GATC 5 cut(s) 168, 786, 1057, 1367, 1578
LmnI GCTCC 6 cut(s) 23, 310, 380, 917, 1452, 1666
Lsp1109I GCAGC 9 cut(s) 44, 248, 251, 611, 724, 746, 1063, 1749, 1777
LweI GCATC 9 cut(s) 491, 541, 820, 846, 952, 1347, 1449, 1484, 1518
MaeI CTAG 4 cut(s) 23, 690, 713, 1397
MaeII ACGT 3 cut(s) 51, 183, 338
MaeIII GTNAC 4 cut(s) 334, 514, 1135, 1462
MalI GATC 5 cut(s) 170, 788, 1059, 1369, 1580
MbiI CCGCTC 1 cut(s) 1154
MboI GATC 5 cut(s) 168, 786, 1057, 1367, 1578
MboII GAAGA 9 cut(s) 449, 979, 1010, 1013, 1106, 1168, 1432, 1507, 1714
MfeI CAATTG 1 cut(s) 1680
MflI RGATCY 1 cut(s) 786
MhlI GDGCHC 1 cut(s) 276
MlsI TGGCCA 2 cut(s) 5, 1191
MluCI AATT 8 cut(s) 453, 564, 674, 821, 1041, 1319, 1349, 1680
MluNI TGGCCA 2 cut(s) 5, 1191
MlyI GAGTC 4 cut(s) 20, 86, 122, 962
MmeI TCCRAC 2 cut(s) 397, 988
Mox20I TGGCCA 2 cut(s) 5, 1191
Mph1103I ATGCAT 1 cut(s) 967
MscI TGGCCA 2 cut(s) 5, 1191
MseI TTAA 4 cut(s) 207, 212, 672, 1308
MslI CAYNNNNRTG 2 cut(s) 1727, 1743
Msp20I TGGCCA 2 cut(s) 5, 1191
MspA1I CMGCKG 6 cut(s) 125, 293, 722, 737, 1268, 1790
MspI CCGG 7 cut(s) 38, 198, 282, 307, 479, 1293, 1331
MspR9I CCNGG 8 cut(s) 161, 198, 199, 283, 307, 308, 636, 1460
MunI CAATTG 1 cut(s) 1680
Mva1269I GAATGC 1 cut(s) 639
MvaI CCWGG 3 cut(s) 161, 636, 1460
MvnI CGCG 2 cut(s) 154, 1428
MwoI GCNNNNNNNGC 8 cut(s) 287, 624, 728, 737, 809, 1216, 1274, 1283
NciI CCSGG 5 cut(s) 198, 199, 283, 307, 308
NdeII GATC 5 cut(s) 168, 786, 1057, 1367, 1578
NlaIII CATG 4 cut(s) 560, 809, 856, 1562
NlaIV GGNNCC 2 cut(s) 19, 445
NmuCI GTSAC 4 cut(s) 334, 514, 1135, 1462
NsiI ATGCAT 1 cut(s) 967
PauI GCGCGC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 639
PfeI GAWTC 3 cut(s) 1173, 1298, 1489
PinAI ACCGGT 1 cut(s) 1292
PleI GAGTC 4 cut(s) 20, 86, 122, 961
PpsI GAGTC 4 cut(s) 20, 86, 122, 961
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 3 cut(s) 159, 634, 1458
PspGI CCWGG 3 cut(s) 159, 634, 1458
PspN4I GGNNCC 2 cut(s) 19, 445
PspPI GGNCC 5 cut(s) 422, 779, 980, 1079, 1565
PsrI GAACNNNNNNTAC 2 cut(s) 1577, 1609
PsuI RGATCY 1 cut(s) 786
PteI GCGCGC 1 cut(s) 152
PvuII CAGCTG 3 cut(s) 737, 1268, 1790
RsaI GTAC 2 cut(s) 76, 1657
RsaNI GTAC 2 cut(s) 75, 1656
RseI CAYNNNNRTG 2 cut(s) 1727, 1743
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 4 cut(s) 207, 212, 672, 1308
Sau3AI GATC 5 cut(s) 168, 786, 1057, 1367, 1578
Sau96I GGNCC 5 cut(s) 422, 779, 980, 1079, 1565
SchI GAGTC 4 cut(s) 20, 86, 122, 962
ScrFI CCNGG 8 cut(s) 161, 198, 199, 283, 307, 308, 636, 1460
SduI GDGCHC 1 cut(s) 276
SfaNI GCATC 9 cut(s) 491, 541, 820, 846, 952, 1347, 1449, 1484, 1518
SfcI CTRYAG 1 cut(s) 997
SinI GGWCC 2 cut(s) 779, 980
SmaI CCCGGG 2 cut(s) 199, 308
SmiMI CAYNNNNRTG 2 cut(s) 1727, 1743
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 8 cut(s) 453, 564, 674, 821, 1041, 1319, 1349, 1680
SspI AATATT 1 cut(s) 13
SspMI CTAG 4 cut(s) 23, 690, 713, 1397
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 8 cut(s) 159, 196, 197, 281, 305, 306, 634, 1458
TaaI ACNGT 6 cut(s) 193, 346, 389, 514, 938, 1410
TaiI ACGT 3 cut(s) 54, 186, 341
TaqI TCGA 2 cut(s) 1370, 1492
TasI AATT 8 cut(s) 453, 564, 674, 821, 1041, 1319, 1349, 1680
TauI GCSGC 6 cut(s) 144, 159, 293, 722, 734, 1803
TfiI GAWTC 3 cut(s) 1173, 1298, 1489
Tru1I TTAA 4 cut(s) 207, 212, 672, 1308
Tru9I TTAA 4 cut(s) 207, 212, 672, 1308
TscAI CASTG 5 cut(s) 394, 762, 841, 1279, 1603
TseFI GTSAC 4 cut(s) 334, 514, 1135, 1462
TseI GCWGC 9 cut(s) 57, 261, 264, 624, 734, 737, 1051, 1762, 1790
Tsp45I GTSAC 4 cut(s) 334, 514, 1135, 1462
TspDTI ATGAA 5 cut(s) 573, 822, 1118, 1362, 1711
TspGWI ACGGA 3 cut(s) 96, 1688, 1709
TspMI CCCGGG 2 cut(s) 197, 306
TspRI CASTG 5 cut(s) 394, 762, 841, 1279, 1603
VpaK11BI GGWCC 2 cut(s) 779, 980
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 4 cut(s) 453, 674, 821, 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 1361
XmaI CCCGGG 2 cut(s) 197, 306
XspI CTAG 4 cut(s) 23, 690, 713, 1397
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.