FvH4_3g39060

Belongs to the TPP enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001627
Physical Location & Seq
Forward (+)
33292884 .. 33295781
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39060.t2

Sequence Viewer

Length: 1815 bp
ATGGCGACTGGCCTTGGCTCCCTAGAGTGTATCAAACCCACCAACAACAACGTAGGCTGCCCTCCCCAAAACGGAGCCTCTGCACTCCAGGACCAGCCTCTCTCCACCATATCCTGCCCTGACTCCACCCTCGGCCGCCACCTGGCTCGCCGCCTCGTGGAGATTGGTGTCAGTGATGTGTTCTCTGTCCCTGGTGACTTTAACCTAACCCTTCTTGACCACCTCATTGCCGAGCCAGGGCTCACTAACATTGGCTGCTGCAATGAGCTCAATGCTGGCTACGCCGCGGACGGATACGCACGGTCTCGCGGCGTAGGGGCTTGTGTGGTCACATTTACTGTTGGTGGCCTTAGCGTGCTTAATGCCATTGCTGGGGCTTATAGTGAGAATCTACCTGTGATTTGTGTTGTTGGAGGACCTAACACAAATGATTTTGGTACCAATAGAATTCTCCACCACACTATCGGCTTGCCGGATTTTAGCCAGGAGCTCCGGTGCTTCCAGACCATCACATGCTATCAAGCTGTGGTGAACAACTTGGAAGATGCGCATGAGCAGATTGATACAGCCATATCTACTGCTCTCAAGGAAAGCAAGCCTGTCTATATTAGCATTAGTTGTAATTTGCCTGGGATTCCACATCCAACTTTTGGCAAAGACCCTATTCCATTTTCATTATCACCTAGAATGAGTAACAAGAGGGGGCTAGAGGCAGCTGTGGAGGCAGCAGCAGCTTTCCTAAACAAGGCAGTGAAGCCAGTCATGGTTGGAGGACCAAAGCTCCGAGTTGCAAAAGCTCATGAAGCGTTTATCGAACTGGCTGATGCCAGTGGCTACGCCCTAGCTGTGATGCCATCAGCCAAAGGCCATGTAGTAGAAACTCACCCTCACTTCATTGGAACATACTGGGGAGCTGTGGGAACTGCCTACTGTGGTGAGATTGTGGAGTCAGCAGATGCATACGTGTTTGTTGGTCCAATTTTTAATGACTACAGCTCTGTTGGGTACTCACTCCTCCTCAAGAAGGAGAAGGCCATCATTGTGCAGCCCGATCGTGTCGTTATAGCGAATGGCCCTTCTTTCGGTTGTGTTCTAATGAAGGACTTCCTCCAGGCACTTGCAAAGAAGATCACTCGCAACACGACTGCTTATGAGAACTACCACAGGATCTTTGTCCCGGACGGCCAGCCTCTCAAATGTGAGCCTGATGAGCCTTTGAGAGTGAATGTTCTGTTCCAACATATTCAGAAAATGCTTTCAGCTGATACAGCTGTTATTGCTGAGACTGGTGACTCTTGGTTTAACTGCCAGAAACTGAAGTTACCAGAAGGATGTGGGTACGAATTCCAGATGCAATATGGTTCGATTGGGTGGTCTGTTGGAGGAACTCTTGGATATGCACAGGCAGTACCGAATAAGCGTGTCATTGCTTGCATTGGTGATGGTAGCTTTCAGGTGACTGCACAAGATGTGTCAACAATGATCAGATGTAATCAGAAGAGCATCATTTTCCTCATAAACAATGGTGGATACACAATAGAAGTCGAAATCCATGACGGGCCTTACAATGTGATCAAGAACTGGAACTACACTGGTTTAGTGGATGCAATCCACAATGGTGAAGGAAATTGCTGGACAACCAAGGTACGCACTGAAGAGGACCTTATAGAAGCCATTGAAACAGCAACAGGGGAGAAGAAAGACTGCTTGTGCTTCATAGAAATCATTGTTCACAAGGATGACACTAGCAAAGAGCTTCTTGAATGGGGTTCCAGGGTCTCTTCTGCCAATAGCCGCCCACCAAATCCGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01130 map00010 map01100 map01110 map01130
Pfam Domains
Protein Families
TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

605

Amino Acids

65.15

Weight (kDa)

5.5

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPP_enzyme_N PF02776 43 - 147 1.5e-28 Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M PF00205 240 - 357 3e-22 Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C PF02775 455 - 578 6e-14 Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 549
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 437
AccB7I CCANNNNNTGG 1 cut(s) 650
AccII CGCG 2 cut(s) 287, 309
AciI CCGC 7 cut(s) 136, 151, 285, 287, 309, 1795, 1808
AclWI GGATC 1 cut(s) 1175
AcoI YGGCCR 2 cut(s) 133, 1183
AcsI RAATTY 2 cut(s) 447, 1343
AcuI CTGAAG 2 cut(s) 1337, 1674
AfaI GTAC 5 cut(s) 439, 1007, 1340, 1410, 1647
AfiI CCNNNNNNNGG 9 cut(s) 71, 142, 157, 237, 372, 650, 745, 1024, 1082
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 2 cut(s) 1679, 1763
AjnI CCWGG 8 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1772
AleI CACNNNNGTG 1 cut(s) 1617
Alw21I GWGCWC 2 cut(s) 270, 492
Alw26I GTCTC 3 cut(s) 309, 1277, 1783
AlwI GGATC 1 cut(s) 1175
AlwNI CAGNNNCTG 1 cut(s) 1315
AoxI GGCC 8 cut(s) 10, 133, 346, 865, 1032, 1072, 1183, 1559
ApeKI GCWGC 8 cut(s) 57, 255, 258, 713, 725, 728, 731, 1045
ApoI RAATTY 2 cut(s) 447, 1343
Asp700I GAANNNNTTC 1 cut(s) 1103
Asp718I GGTACC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 7 cut(s) 91, 416, 773, 974, 1073, 1559, 1660
AsuC2I CCSGG 1 cut(s) 1178
AsuHPI GGTGA 9 cut(s) 206, 541, 672, 875, 947, 1301, 1451, 1468, 1631
AvaII GGWCC 5 cut(s) 91, 416, 773, 974, 1660
BaeI ACNNNNGTAYC 2 cut(s) 1257, 1290
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 3 cut(s) 243, 270, 492
BauI CACGAG 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 270, 492
BbvI GCAGC 8 cut(s) 44, 242, 245, 725, 737, 740, 743, 1057
BccI CCATC 4 cut(s) 515, 862, 1043, 1436
BceAI ACGGC 1 cut(s) 1198
BcgI CGANNNNNNTGC 2 cut(s) 1034, 1068
BciT130I CCWGG 8 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1774
BciVI GTATCC 2 cut(s) 287, 1523
BclI TGATCA 2 cut(s) 1482, 1572
BcnI CCSGG 1 cut(s) 1178
BcoDI GTCTC 3 cut(s) 309, 1277, 1783
BfaI CTAG 5 cut(s) 23, 684, 707, 842, 1746
BfmI CTRYAG 1 cut(s) 991
BfuI GTATCC 2 cut(s) 287, 1523
Bme1390I CCNGG 9 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1178, 1774
Bme18I GGWCC 5 cut(s) 91, 416, 773, 974, 1660
BmgT120I GGNCC 7 cut(s) 91, 416, 773, 974, 1073, 1559, 1660
BmiI GGNNCC 4 cut(s) 19, 76, 439, 1771
BmrFI CCNGG 9 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1178, 1774
BmrI ACTGGG 1 cut(s) 916
BmsI GCATC 7 cut(s) 535, 814, 840, 946, 1341, 1512, 1594
BmuI ACTGGG 1 cut(s) 916
BpmI CTGGAG 2 cut(s) 71, 1094
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 569, 1004
BpuMI CCSGG 1 cut(s) 1178
BsaAI YACGTR 1 cut(s) 964
BsaI GGTCTC 2 cut(s) 309, 1783
BsaJI CCNNGG 8 cut(s) 13, 130, 190, 236, 285, 629, 1641, 1773
BsaWI WCCGGW 1 cut(s) 492
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 765, 795
Bsc4I CCNNNNNNNGG 9 cut(s) 71, 142, 157, 237, 372, 650, 745, 1024, 1082
Bse1I ACTGG 8 cut(s) 13, 758, 822, 828, 911, 1291, 1586, 1597
Bse3DI GCAATG 4 cut(s) 225, 268, 366, 1425
BseBI CCWGG 8 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1774
BseDI CCNNGG 8 cut(s) 13, 130, 190, 236, 285, 629, 1641, 1773
BseGI GGATG 4 cut(s) 640, 1337, 1609, 1744
BseLI CCNNNNNNNGG 9 cut(s) 71, 142, 157, 237, 372, 650, 745, 1024, 1082
BseMI GCAATG 4 cut(s) 225, 268, 366, 1425
BseMII CTCAG 1 cut(s) 1272
BseNI ACTGG 8 cut(s) 13, 758, 822, 828, 911, 1291, 1586, 1597
BseRI GAGGAG 2 cut(s) 1004, 1007
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 8 cut(s) 44, 242, 245, 725, 737, 740, 743, 1057
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 3 cut(s) 66, 1064, 1446
Bsh1236I CGCG 2 cut(s) 287, 309
Bsh1285I CGRYCG 2 cut(s) 136, 1054
BshFI GGCC 8 cut(s) 12, 135, 348, 867, 1034, 1074, 1185, 1561
BshNI GGYRCC 1 cut(s) 437
BsiEI CGRYCG 2 cut(s) 136, 1054
BsiHKAI GWGCWC 2 cut(s) 270, 492
BsiSI CCGG 3 cut(s) 473, 493, 1178
BslFI GGGAC 2 cut(s) 173, 1160
BslI CCNNNNNNNGG 9 cut(s) 71, 142, 157, 237, 372, 650, 745, 1024, 1082
BsmAI GTCTC 3 cut(s) 309, 1277, 1783
BsmFI GGGAC 2 cut(s) 173, 1160
BsnI GGCC 8 cut(s) 12, 135, 348, 867, 1034, 1074, 1185, 1561
Bso31I GGTCTC 2 cut(s) 309, 1783
Bsp1286I GDGCHC 3 cut(s) 243, 270, 492
Bsp143I GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
BspACI CCGC 7 cut(s) 136, 151, 285, 287, 309, 1795, 1808
BspANI GGCC 8 cut(s) 12, 135, 348, 867, 1034, 1074, 1185, 1561
BspCNI CTCAG 1 cut(s) 1273
BspFNI CGCG 2 cut(s) 287, 309
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 4 cut(s) 19, 76, 439, 1771
BspPI GGATC 1 cut(s) 1175
BspQI GCTCTTC 1 cut(s) 1493
BspT107I GGYRCC 1 cut(s) 437
BspTNI GGTCTC 2 cut(s) 309, 1783
BsrDI GCAATG 4 cut(s) 225, 268, 366, 1425
BsrI ACTGG 8 cut(s) 13, 758, 822, 828, 911, 1291, 1586, 1597
BssECI CCNNGG 8 cut(s) 13, 130, 190, 236, 285, 629, 1641, 1773
BssMI GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 2 cut(s) 13, 1641
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 8 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1774
Bst4CI ACNGT 3 cut(s) 303, 340, 932
Bst6I CTCTTC 3 cut(s) 1493, 1650, 1786
BstBAI YACGTR 1 cut(s) 964
BstC8I GCNNGC 7 cut(s) 148, 277, 356, 470, 596, 1187, 1432
BstDEI CTNAG 2 cut(s) 350, 1281
BstDSI CCRYGG 1 cut(s) 285
BstENI CCTNNNNNAGG 2 cut(s) 743, 1022
BstF5I GGATG 4 cut(s) 640, 1337, 1609, 1744
BstFNI CGCG 2 cut(s) 287, 309
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 5 cut(s) 1054, 1131, 1170, 1485, 1575
BstMAI GTCTC 3 cut(s) 309, 1277, 1783
BstMBI GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
BstMCI CGRYCG 2 cut(s) 136, 1054
BstMWI GCNNNNNNNGC 7 cut(s) 281, 722, 731, 803, 1210, 1268, 1277
BstNI CCWGG 8 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1774
BstNSI RCATGY 1 cut(s) 516
BstSCI CCNGG 9 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1176, 1772
BstSFI CTRYAG 1 cut(s) 991
BstUI CGCG 2 cut(s) 287, 309
BstV1I GCAGC 8 cut(s) 44, 242, 245, 725, 737, 740, 743, 1057
BstX2I RGATCY 1 cut(s) 1167
BstYI RGATCY 1 cut(s) 1167
BstZI CGGCCG 1 cut(s) 133
BsuI GTATCC 2 cut(s) 287, 1523
BsuRI GGCC 8 cut(s) 12, 135, 348, 867, 1034, 1074, 1185, 1561
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 4 cut(s) 640, 1337, 1609, 1744
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 5 cut(s) 178, 756, 835, 1590, 1650
Cac8I GCNNGC 7 cut(s) 148, 277, 356, 470, 596, 1187, 1432
CaiI CAGNNNCTG 1 cut(s) 1315
CciI TCATGA 1 cut(s) 799
CfoI GCGC 1 cut(s) 550
Cfr13I GGNCC 7 cut(s) 91, 416, 773, 974, 1073, 1559, 1660
Cfr42I CCGCGG 1 cut(s) 288
Csp6I GTAC 5 cut(s) 438, 1006, 1339, 1409, 1646
CviAII CATG 6 cut(s) 513, 551, 763, 800, 869, 1553
CviQI GTAC 5 cut(s) 438, 1006, 1339, 1409, 1646
DdeI CTNAG 2 cut(s) 350, 1281
DpnI GATC 5 cut(s) 1053, 1130, 1169, 1484, 1574
DpnII GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
EaeI YGGCCR 2 cut(s) 133, 1183
EagI CGGCCG 1 cut(s) 133
Eam1104I CTCTTC 3 cut(s) 1493, 1650, 1786
EarI CTCTTC 3 cut(s) 1493, 1650, 1786
Ecl136II GAGCTC 2 cut(s) 268, 490
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 13, 1641
Eco24I GRGCYC 3 cut(s) 243, 270, 492
Eco31I GGTCTC 2 cut(s) 309, 1783
Eco47I GGWCC 5 cut(s) 91, 416, 773, 974, 1660
Eco52I CGGCCG 1 cut(s) 133
Eco53kI GAGCTC 2 cut(s) 268, 490
Eco57I CTGAAG 2 cut(s) 1337, 1674
EcoICRI GAGCTC 2 cut(s) 268, 490
EcoNI CCTNNNNNAGG 2 cut(s) 743, 1022
EcoO109I RGGNCCY 2 cut(s) 416, 1660
EcoRI GAATTC 2 cut(s) 447, 1343
EcoRII CCWGG 8 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1772
EcoT14I CCWWGG 2 cut(s) 13, 1641
EcoT22I ATGCAT 1 cut(s) 961
EcoT38I GRGCYC 3 cut(s) 243, 270, 492
ErhI CCWWGG 2 cut(s) 13, 1641
FaeI CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
FalI AAGNNNNNCTT 4 cut(s) 1647, 1679, 1743, 1775
FaqI GGGAC 2 cut(s) 173, 1160
FatI CATG 6 cut(s) 512, 550, 762, 799, 868, 1552
FbaI TGATCA 2 cut(s) 1482, 1572
FokI GGATG 4 cut(s) 627, 1344, 1616, 1751
FriOI GRGCYC 3 cut(s) 243, 270, 492
FspAI RTGCGCAY 1 cut(s) 549
FspBI CTAG 5 cut(s) 23, 684, 707, 842, 1746
FspI TGCGCA 1 cut(s) 549
GlaI GCGC 1 cut(s) 549
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 2 cut(s) 71, 1094
HaeIII GGCC 8 cut(s) 12, 135, 348, 867, 1034, 1074, 1185, 1561
HapII CCGG 3 cut(s) 473, 493, 1178
HhaI GCGC 1 cut(s) 550
Hin1II CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 1 cut(s) 1476
HindII GTYRAC 1 cut(s) 1476
HinfI GANTC 5 cut(s) 122, 388, 634, 947, 1292
HpaII CCGG 3 cut(s) 473, 493, 1178
HphI GGTGA 9 cut(s) 206, 541, 672, 875, 947, 1301, 1451, 1468, 1631
Hpy166II GTNNAC 3 cut(s) 532, 1476, 1732
Hpy188I TCNGA 4 cut(s) 785, 1248, 1487, 1497
Hpy188III TCNNGA 7 cut(s) 215, 502, 800, 1021, 1348, 1576, 1760
Hpy8I GTNNAC 3 cut(s) 532, 1476, 1732
HpyAV CCTTC 7 cut(s) 221, 1018, 1024, 1086, 1093, 1322, 1616
HpyCH4III ACNGT 3 cut(s) 303, 340, 932
HpyCH4IV ACGT 2 cut(s) 51, 963
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 722, 731, 803, 1210, 1268, 1277
HpyF3I CTNAG 2 cut(s) 350, 1281
HpySE526I ACGT 2 cut(s) 51, 963
Hsp92II CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
HspAI GCGC 1 cut(s) 548
KpnI GGTACC 1 cut(s) 441
Ksp22I TGATCA 2 cut(s) 1482, 1572
KspI CCGCGG 1 cut(s) 288
Kzo9I GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
LguI GCTCTTC 1 cut(s) 1493
LmnI GCTCC 6 cut(s) 23, 74, 487, 495, 786, 911
Lsp1109I GCAGC 8 cut(s) 44, 242, 245, 725, 737, 740, 743, 1057
LweI GCATC 7 cut(s) 535, 814, 840, 946, 1341, 1512, 1594
MaeI CTAG 5 cut(s) 23, 684, 707, 842, 1746
MaeII ACGT 2 cut(s) 51, 963
MaeIII GTNAC 6 cut(s) 194, 328, 692, 1289, 1320, 1456
MalI GATC 5 cut(s) 1053, 1130, 1169, 1484, 1574
MboI GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
MboII GAAGA 6 cut(s) 554, 1138, 1510, 1667, 1708, 1773
MflI RGATCY 1 cut(s) 1167
MhlI GDGCHC 3 cut(s) 243, 270, 492
MluCI AATT 5 cut(s) 447, 622, 978, 1343, 1627
MlyI GAGTC 3 cut(s) 116, 956, 1286
MmeI TCCRAC 5 cut(s) 391, 668, 748, 1261, 1360
Mph1103I ATGCAT 1 cut(s) 961
MroXI GAANNNNTTC 1 cut(s) 1103
MseI TTAA 4 cut(s) 201, 360, 984, 1302
MslI CAYNNNNRTG 3 cut(s) 1040, 1617, 1737
MspA1I CMGCKG 4 cut(s) 287, 716, 1262, 1271
MspI CCGG 3 cut(s) 473, 493, 1178
MspR9I CCNGG 9 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1178, 1774
MvaI CCWGG 8 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1774
MvnI CGCG 2 cut(s) 287, 309
MwoI GCNNNNNNNGC 7 cut(s) 281, 722, 731, 803, 1210, 1268, 1277
NciI CCSGG 1 cut(s) 1178
NdeII GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
NlaIII CATG 6 cut(s) 516, 554, 766, 803, 872, 1556
NlaIV GGNNCC 4 cut(s) 19, 76, 439, 1771
NmeAIII GCCGAG 2 cut(s) 111, 256
NmuCI GTSAC 4 cut(s) 194, 328, 1289, 1456
NsbI TGCGCA 1 cut(s) 549
NsiI ATGCAT 1 cut(s) 961
NspI RCATGY 1 cut(s) 516
OliI CACNNNNGTG 1 cut(s) 1617
PagI TCATGA 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 1493
PdmI GAANNNNTTC 1 cut(s) 1103
PfeI GAWTC 2 cut(s) 388, 634
PflMI CCANNNNNTGG 1 cut(s) 650
PfoI TCCNGGA 2 cut(s) 87, 1176
Ple19I CGATCG 1 cut(s) 1054
PleI GAGTC 3 cut(s) 116, 955, 1286
PpsI GAGTC 3 cut(s) 116, 955, 1286
Ppu21I YACGTR 1 cut(s) 964
PpuMI RGGWCCY 2 cut(s) 416, 1660
Psp124BI GAGCTC 2 cut(s) 270, 492
Psp5II RGGWCCY 2 cut(s) 416, 1660
Psp6I CCWGG 8 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1772
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 8 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1772
PspN4I GGNNCC 4 cut(s) 19, 76, 439, 1771
PspPI GGNCC 7 cut(s) 91, 416, 773, 974, 1073, 1559, 1660
PspPPI RGGWCCY 2 cut(s) 416, 1660
PsrI GAACNNNNNNTAC 2 cut(s) 1571, 1603
PstNI CAGNNNCTG 1 cut(s) 1315
PsuI RGATCY 1 cut(s) 1167
PvuI CGATCG 1 cut(s) 1054
PvuII CAGCTG 3 cut(s) 716, 1262, 1271
RsaI GTAC 5 cut(s) 439, 1007, 1340, 1410, 1647
RsaNI GTAC 5 cut(s) 438, 1006, 1339, 1409, 1646
RseI CAYNNNNRTG 3 cut(s) 1040, 1617, 1737
SacI GAGCTC 2 cut(s) 270, 492
SacII CCGCGG 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 1493
SaqAI TTAA 4 cut(s) 201, 360, 984, 1302
Sau3AI GATC 5 cut(s) 1051, 1128, 1167, 1482, 1572
Sau96I GGNCC 7 cut(s) 91, 416, 773, 974, 1073, 1559, 1660
SchI GAGTC 3 cut(s) 116, 956, 1286
ScrFI CCNGG 9 cut(s) 89, 143, 192, 237, 485, 630, 1112, 1178, 1774
SduI GDGCHC 3 cut(s) 243, 270, 492
SfaNI GCATC 7 cut(s) 535, 814, 840, 946, 1341, 1512, 1594
SfcI CTRYAG 1 cut(s) 991
Sfr303I CCGCGG 1 cut(s) 288
SgrBI CCGCGG 1 cut(s) 288
SinI GGWCC 5 cut(s) 91, 416, 773, 974, 1660
SmiMI CAYNNNNRTG 3 cut(s) 1040, 1617, 1737
SmlI CTYRAG 2 cut(s) 584, 1019
SmoI CTYRAG 2 cut(s) 584, 1019
Sse9I AATT 5 cut(s) 447, 622, 978, 1343, 1627
SsiI CCGC 7 cut(s) 136, 151, 285, 287, 309, 1795, 1808
SspMI CTAG 5 cut(s) 23, 684, 707, 842, 1746
SstI GAGCTC 2 cut(s) 270, 492
StyD4I CCNGG 9 cut(s) 87, 141, 190, 235, 483, 628, 1110, 1176, 1772
StyI CCWWGG 2 cut(s) 13, 1641
TaaI ACNGT 3 cut(s) 303, 340, 932
TaiI ACGT 2 cut(s) 54, 966
TaqI TCGA 3 cut(s) 813, 1364, 1545
TasI AATT 5 cut(s) 447, 622, 978, 1343, 1627
TauI GCSGC 5 cut(s) 138, 153, 287, 312, 1797
TfiI GAWTC 2 cut(s) 388, 634
Tru1I TTAA 4 cut(s) 201, 360, 984, 1302
Tru9I TTAA 4 cut(s) 201, 360, 984, 1302
TscAI CASTG 5 cut(s) 178, 756, 835, 1597, 1657
TseFI GTSAC 4 cut(s) 194, 328, 1289, 1456
TseI GCWGC 8 cut(s) 57, 255, 258, 713, 725, 728, 731, 1045
Tsp45I GTSAC 4 cut(s) 194, 328, 1289, 1456
TspDTI ATGAA 5 cut(s) 663, 816, 883, 1112, 1705
TspGWI ACGGA 2 cut(s) 87, 306
TspRI CASTG 5 cut(s) 178, 756, 835, 1597, 1657
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 5 cut(s) 91, 416, 773, 974, 1660
XagI CCTNNNNNAGG 2 cut(s) 743, 1022
XapI RAATTY 2 cut(s) 447, 1343
XceI RCATGY 1 cut(s) 516
XcmI CCANNNNNNNNNTGG 1 cut(s) 1355
XmnI GAANNNNTTC 1 cut(s) 1103
XspI CTAG 5 cut(s) 23, 684, 707, 842, 1746
Zsp2I ATGCAT 1 cut(s) 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.