FvH4_3g17480

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003122
Physical Location & Seq
Reverse (-)
11136911 .. 11147297
10387 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g17480.t2

Sequence Viewer

Length: 624 bp
ATGATGAAGTCCATGAAGATGATCGGAAGCTTGAGCTGTTGCATGTGGCTAGTGATGGCCATGGTAACTTCAGCCCAAGATAACGCTCCGGTGCTCGACACCTCCGGGCAGGCTCTTCAATCCGGTGTAGACTACTTCATTAAACCCGCCATCACCGACATCGGCGGCCGTTTCACTTTGATCGACCGAAACGACTCGTGTCCTTTATATGTTGGTCAAAAAAACACCTCCGGCTCCGAGGACTTCCCGGTGACCTTTGCGCCTTTCGTAGAGGGCGAGACGGTGGTGAGAGAGGGTAGGGATCAGAAGATCACATTCTCTGCAGTCACAACCTGTGTGCAATCAACTACATGGAAGGTGGGTGAGAAGCAGTCCGAGACCCAGAGGAGGTTGATTGTCACCGGAATTGATCAAAACGAAGGAATAGCGGGTCCTGCCGGAAATTACTTCAGGATTAATAAAGATGCAGATATTGATGGCGTCTACAGCCTGCAGTGGTGCCCTACTGAATTGTGCCCGACTTGCAGGTTCATTTGCGGAGATGTGGGTGCTTTGCTTGAGAATGGCAAGAGGTTGTTGGCCTTGGATGGTTCTGCGCTTCCTGTTGTCTTCGAGAGGGCTTAA

Protein Analysis

208

Amino Acids

22.53

Weight (kDa)

4.83

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 31 - 205 3.2e-47 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 1 cut(s) 498
AccI GTMKAC 2 cut(s) 129, 483
AciI CCGC 4 cut(s) 147, 165, 428, 537
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 2 cut(s) 57, 166
AcuI CTGAAG 2 cut(s) 54, 433
AcyI GRCGYC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 387
AgsI TTSAA 1 cut(s) 119
AluBI AGCT 2 cut(s) 30, 36
AluI AGCT 2 cut(s) 30, 36
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 272, 371
AlwI GGATC 1 cut(s) 309
AoxI GGCC 3 cut(s) 57, 166, 579
AseI ATTAAT 1 cut(s) 456
AspLEI GCGC 2 cut(s) 262, 598
AspS9I GGNCC 1 cut(s) 431
AsuC2I CCSGG 2 cut(s) 106, 248
AsuHPI GGTGA 5 cut(s) 145, 262, 298, 374, 391
AvaII GGWCC 1 cut(s) 431
BaeGI GKGCMC 2 cut(s) 503, 518
BalI TGGCCA 1 cut(s) 59
BanI GGYRCC 1 cut(s) 498
BauI CACGAG 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 601
Bbv12I GWGCWC 1 cut(s) 96
BccI CCATC 4 cut(s) 49, 158, 470, 581
BceAI ACGGC 1 cut(s) 153
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 2 cut(s) 106, 248
BcoDI GTCTC 2 cut(s) 272, 371
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 3 cut(s) 321, 484, 491
BfuAI ACCTGC 1 cut(s) 516
BisI GCNGC 1 cut(s) 166
BlsI GCNGC 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 106, 248
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 3 cut(s) 235, 432, 500
BmrFI CCNGG 2 cut(s) 106, 248
BmsI GCATC 1 cut(s) 454
BoxI GACNNNNGTC 1 cut(s) 198
BpiI GAAGAC 1 cut(s) 601
BpuEI CTTGAG 2 cut(s) 52, 578
BpuMI CCSGG 2 cut(s) 106, 248
BsaHI GRCGYC 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 60, 237, 582
BsaWI WCCGGW 3 cut(s) 88, 122, 401
Bsc4I CCNNNNNNNGG 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 60, 237, 582
BseGI GGATG 1 cut(s) 592
BseLI CCNNNNNNNGG 1 cut(s) 387
BseRI GAGGAG 1 cut(s) 400
BseSI GKGCMC 2 cut(s) 503, 518
BseX3I CGGCCG 1 cut(s) 166
Bsh1285I CGRYCG 2 cut(s) 169, 187
BshFI GGCC 3 cut(s) 59, 168, 581
BshNI GGYRCC 1 cut(s) 498
BsiEI CGRYCG 2 cut(s) 169, 187
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 7 cut(s) 89, 105, 123, 231, 248, 402, 438
BslI CCNNNNNNNGG 1 cut(s) 387
BsmAI GTCTC 2 cut(s) 272, 371
BsmBI CGTCTC 1 cut(s) 272
BsnI GGCC 3 cut(s) 59, 168, 581
Bso31I GGTCTC 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 96, 503, 518
Bsp143I GATC 5 cut(s) 21, 180, 301, 309, 409
Bsp19I CCATGG 1 cut(s) 60
BspACI CCGC 4 cut(s) 147, 165, 428, 537
BspANI GGCC 3 cut(s) 59, 168, 581
BspLI GGNNCC 3 cut(s) 235, 432, 500
BspMAI CTGCAG 2 cut(s) 325, 495
BspMI ACCTGC 1 cut(s) 516
BspPI GGATC 1 cut(s) 309
BspQI GCTCTTC 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 498
BspTNI GGTCTC 1 cut(s) 371
BssECI CCNNGG 3 cut(s) 60, 237, 582
BssMI GATC 5 cut(s) 21, 180, 301, 309, 409
BssNI GRCGYC 1 cut(s) 480
BssSI CACGAG 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 60, 582
Bst2BI CACGAG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 480
BstC8I GCNNGC 2 cut(s) 111, 491
BstDSI CCRYGG 1 cut(s) 60
BstEII GGTNACC 1 cut(s) 250
BstF5I GGATG 1 cut(s) 592
BstHHI GCGC 2 cut(s) 262, 598
BstKTI GATC 5 cut(s) 24, 183, 304, 312, 412
BstMAI GTCTC 2 cut(s) 272, 371
BstMBI GATC 5 cut(s) 21, 180, 301, 309, 409
BstMCI CGRYCG 2 cut(s) 169, 187
BstMWI GCNNNNNNNGC 3 cut(s) 434, 486, 522
BstNSI RCATGY 1 cut(s) 46
BstPAI GACNNNNGTC 1 cut(s) 198
BstPI GGTNACC 1 cut(s) 250
BstSCI CCNGG 2 cut(s) 104, 246
BstSFI CTRYAG 3 cut(s) 321, 484, 491
BstSLI GKGCMC 2 cut(s) 503, 518
BstV2I GAAGAC 1 cut(s) 601
BstZI CGGCCG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 59, 168, 581
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 592
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 1 cut(s) 500
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 2 cut(s) 111, 491
CfoI GCGC 2 cut(s) 262, 598
Cfr13I GGNCC 1 cut(s) 431
CseI GACGC 1 cut(s) 469
CviAII CATG 4 cut(s) 13, 43, 61, 351
DpnI GATC 5 cut(s) 23, 182, 303, 311, 411
DpnII GATC 5 cut(s) 21, 180, 301, 309, 409
EaeI YGGCCR 2 cut(s) 57, 166
EagI CGGCCG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 60, 582
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 431
Eco52I CGGCCG 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 54, 433
Eco91I GGTNACC 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 60, 582
ErhI CCWWGG 2 cut(s) 60, 582
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 4 cut(s) 16, 46, 64, 354
FaiI YATR 6 cut(s) 14, 44, 62, 208, 210, 352
FatI CATG 4 cut(s) 12, 42, 60, 350
FauI CCCGC 2 cut(s) 154, 421
FbaI TGATCA 1 cut(s) 409
FblI GTMKAC 2 cut(s) 129, 483
Fnu4HI GCNGC 1 cut(s) 166
FokI GGATG 1 cut(s) 599
Fsp4HI GCNGC 1 cut(s) 166
FspBI CTAG 1 cut(s) 50
GlaI GCGC 2 cut(s) 261, 597
GluI GCNGC 1 cut(s) 166
HaeIII GGCC 3 cut(s) 59, 168, 581
HapII CCGG 7 cut(s) 89, 105, 123, 231, 248, 402, 438
HgaI GACGC 1 cut(s) 469
HhaI GCGC 2 cut(s) 262, 598
Hin1I GRCGYC 1 cut(s) 480
Hin1II CATG 4 cut(s) 16, 46, 64, 354
Hin6I GCGC 2 cut(s) 260, 596
HinP1I GCGC 2 cut(s) 260, 596
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 1 cut(s) 194
HpaII CCGG 7 cut(s) 89, 105, 123, 231, 248, 402, 438
HphI GGTGA 5 cut(s) 145, 262, 298, 374, 391
Hpy166II GTNNAC 2 cut(s) 130, 484
Hpy188I TCNGA 4 cut(s) 26, 238, 306, 376
Hpy188III TCNNGA 2 cut(s) 451, 613
Hpy8I GTNNAC 2 cut(s) 130, 484
HpyAV CCTTC 2 cut(s) 349, 413
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4V TGCA 6 cut(s) 42, 323, 340, 467, 493, 525
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 486, 522
Hsp92I GRCGYC 1 cut(s) 480
Hsp92II CATG 4 cut(s) 16, 46, 64, 354
HspAI GCGC 2 cut(s) 260, 596
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 21, 180, 301, 309, 409
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 2 cut(s) 91, 239
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 4 cut(s) 64, 250, 325, 397
MalI GATC 5 cut(s) 23, 182, 303, 311, 411
MboI GATC 5 cut(s) 21, 180, 301, 309, 409
MboII GAAGA 4 cut(s) 28, 107, 319, 601
MhlI GDGCHC 3 cut(s) 96, 503, 518
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 3 cut(s) 405, 442, 509
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 9 cut(s) 112, 232, 238, 265, 286, 378, 381, 564, 609
Mox20I TGGCCA 1 cut(s) 59
MscI TGGCCA 1 cut(s) 59
MseI TTAA 3 cut(s) 141, 456, 622
MslI CAYNNNNRTG 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 59
MspI CCGG 7 cut(s) 89, 105, 123, 231, 248, 402, 438
MspR9I CCNGG 2 cut(s) 106, 248
MwoI GCNNNNNNNGC 3 cut(s) 434, 486, 522
NciI CCSGG 2 cut(s) 106, 248
NcoI CCATGG 1 cut(s) 60
NdeII GATC 5 cut(s) 21, 180, 301, 309, 409
NlaIII CATG 4 cut(s) 16, 46, 64, 354
NlaIV GGNNCC 3 cut(s) 235, 432, 500
NmuCI GTSAC 3 cut(s) 250, 325, 397
NspI RCATGY 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 120
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 273
PkrI GCNGC 1 cut(s) 167
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PpuMI RGGWCCY 1 cut(s) 431
PshAI GACNNNNGTC 1 cut(s) 198
PshBI ATTAAT 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 431
PspEI GGTNACC 1 cut(s) 250
PspN4I GGNNCC 3 cut(s) 235, 432, 500
PspPI GGNCC 1 cut(s) 431
PspPPI RGGWCCY 1 cut(s) 431
PstI CTGCAG 2 cut(s) 325, 495
RseI CAYNNNNRTG 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 141, 456, 622
SatI GCNGC 1 cut(s) 166
Sau3AI GATC 5 cut(s) 21, 180, 301, 309, 409
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 2 cut(s) 106, 248
SduI GDGCHC 3 cut(s) 96, 503, 518
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 3 cut(s) 321, 484, 491
SinI GGWCC 1 cut(s) 431
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 2 cut(s) 31, 557
SmoI CTYRAG 2 cut(s) 31, 557
Sse9I AATT 3 cut(s) 405, 442, 509
SsiI CCGC 4 cut(s) 147, 165, 428, 537
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 104, 246
StyI CCWWGG 2 cut(s) 60, 582
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 3 cut(s) 96, 183, 612
TaqII GACCGA 1 cut(s) 201
TasI AATT 3 cut(s) 405, 442, 509
TauI GCSGC 1 cut(s) 168
Tru1I TTAA 3 cut(s) 141, 456, 622
Tru9I TTAA 3 cut(s) 141, 456, 622
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 3 cut(s) 250, 325, 397
Tsp45I GTSAC 3 cut(s) 250, 325, 397
TspDTI ATGAA 4 cut(s) 20, 29, 127, 520
TspRI CASTG 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 431
VspI ATTAAT 1 cut(s) 456
XceI RCATGY 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 129, 483
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.