Rroxscaffold_1G00050360

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003122
Physical Location & Seq
Reverse (-)
70924809 .. 70925375
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00050360.1

Sequence Viewer

Length: 567 bp
ATGGCCATGGCAACCATAAACCAAACATCTCCGGTGCTCGATACGTCTGGGAAGGCACTTAAATCCGGCATGGATTACTACATCAAACCCGCCAGTACTTACAAAGGTGGTGCTTTAACTCTAATAAACCGGGAGCCATATTTATGTCCACTCTACGTTGGCCAAGGAAATGACTCAGGCTGGCCTTTTCAGTTCAAGCCATTTTTCGACGGAGAAACAGTGGTGAGGGAGAGTAGGGATCAAATGATTATATCTCGTGTCTCTACACATTGCAGGCGGTCAAGTTCATGGTCGATTGAAAAAGCTAACCCAGAGGACCAACGGAAATTAATTGCTATGCAAGAAACTGCAAGAGGAAAACTGCCAAATGGTAAATACTTTAGGATTACTAGATCACAGCCGGAGGTGGATGGTAATAGCTACATTCTGAGATGGTGTCCCACTGATCTGTGTTCTAATTGTACATTCGCTGCTTGTGGAAATATTGGTTCTTTAGATTCGAATGGAGGGAAGAGGTTGTTGGCTTTGAATGGTTCTGAGCTTCCGGTTGTGTTTGAAAGGGCATGA

Protein Analysis

188

Amino Acids

20.84

Weight (kDa)

8.92

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 12 - 186 1.6e-34 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 90, 277
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 2 cut(s) 3, 160
AfaI GTAC 2 cut(s) 97, 463
AgsI TTSAA 4 cut(s) 196, 299, 529, 557
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 3 cut(s) 305, 420, 541
AluI AGCT 3 cut(s) 305, 420, 541
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 1 cut(s) 246
AoxI GGCC 3 cut(s) 3, 160, 182
ApeKI GCWGC 1 cut(s) 470
AseI ATTAAT 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 316
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 235
AsuII TTCGAA 1 cut(s) 500
AvaII GGWCC 1 cut(s) 316
BalI TGGCCA 2 cut(s) 5, 162
BauI CACGAG 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 457
BccI CCATC 2 cut(s) 404, 426
BcnI CCSGG 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 1 cut(s) 390
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 1 cut(s) 131
Bpu14I TTCGAA 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 6, 163
BsaWI WCCGGW 2 cut(s) 31, 544
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse1I ACTGG 1 cut(s) 93
Bse3DI GCAATG 1 cut(s) 268
BseDI CCNNGG 2 cut(s) 6, 163
BseGI GGATG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 3 cut(s) 189, 419, 528
BseNI ACTGG 1 cut(s) 93
BseXI GCAGC 1 cut(s) 457
BshFI GGCC 3 cut(s) 5, 162, 184
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 5 cut(s) 32, 66, 130, 401, 545
BslFI GGGAC 1 cut(s) 423
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 423
BsnI GGCC 3 cut(s) 5, 162, 184
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 1 cut(s) 39
Bsp1407I TGTACA 1 cut(s) 461
Bsp143I GATC 3 cut(s) 238, 392, 445
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 2 cut(s) 90, 277
BspANI GGCC 3 cut(s) 5, 162, 184
BspCNI CTCAG 3 cut(s) 188, 420, 529
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 1 cut(s) 246
BspT104I TTCGAA 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 268
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 6, 163
BssMI GATC 3 cut(s) 238, 392, 445
BssSI CACGAG 1 cut(s) 255
BssT1I CCWWGG 2 cut(s) 6, 163
Bst2BI CACGAG 1 cut(s) 255
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 506
BstAUI TGTACA 1 cut(s) 461
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 182, 275
BstDEI CTNAG 3 cut(s) 175, 428, 537
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 415
BstKTI GATC 3 cut(s) 241, 395, 448
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 3 cut(s) 238, 392, 445
BstSCI CCNGG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 457
BsuRI GGCC 3 cut(s) 5, 162, 184
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 2 cut(s) 225, 441
Cac8I GCNNGC 2 cut(s) 182, 275
Cfr13I GGNCC 1 cut(s) 316
Csp6I GTAC 2 cut(s) 96, 462
CviAII CATG 4 cut(s) 7, 70, 288, 564
CviQI GTAC 2 cut(s) 96, 462
DdeI CTNAG 3 cut(s) 175, 428, 537
DpnI GATC 3 cut(s) 240, 394, 447
DpnII GATC 3 cut(s) 238, 392, 445
EaeI YGGCCR 2 cut(s) 3, 160
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 2 cut(s) 6, 163
Eco47I GGWCC 1 cut(s) 316
EcoT14I CCWWGG 2 cut(s) 6, 163
ErhI CCWWGG 2 cut(s) 6, 163
FaeI CATG 4 cut(s) 10, 73, 291, 567
FaiI YATR 9 cut(s) 8, 17, 71, 139, 145, 251, 289, 338, 565
FaqI GGGAC 1 cut(s) 423
FatI CATG 4 cut(s) 6, 69, 287, 563
FauI CCCGC 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 471
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 1 cut(s) 390
GluI GCNGC 1 cut(s) 471
HaeIII GGCC 3 cut(s) 5, 162, 184
HapII CCGG 5 cut(s) 32, 66, 130, 401, 545
Hin1II CATG 4 cut(s) 10, 73, 291, 567
HinfI GANTC 2 cut(s) 173, 497
HpaII CCGG 5 cut(s) 32, 66, 130, 401, 545
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 149
Hpy188I TCNGA 2 cut(s) 429, 538
Hpy8I GTNNAC 1 cut(s) 149
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 2 cut(s) 44, 156
HpyCH4V TGCA 3 cut(s) 273, 340, 350
HpyF3I CTNAG 3 cut(s) 175, 428, 537
HpySE526I ACGT 2 cut(s) 44, 156
Hsp92II CATG 4 cut(s) 10, 73, 291, 567
Kzo9I GATC 3 cut(s) 238, 392, 445
LmnI GCTCC 1 cut(s) 133
Lsp1109I GCAGC 1 cut(s) 457
MaeI CTAG 1 cut(s) 390
MaeII ACGT 2 cut(s) 44, 156
MalI GATC 3 cut(s) 240, 394, 447
MboI GATC 3 cut(s) 238, 392, 445
MboII GAAGA 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 2 cut(s) 5, 162
MluCI AATT 3 cut(s) 326, 330, 457
MluNI TGGCCA 2 cut(s) 5, 162
MlyI GAGTC 1 cut(s) 167
MnlI CCTC 6 cut(s) 219, 307, 347, 397, 500, 507
Mox20I TGGCCA 2 cut(s) 5, 162
MscI TGGCCA 2 cut(s) 5, 162
MseI TTAA 3 cut(s) 60, 116, 329
MslI CAYNNNNRTG 1 cut(s) 142
Msp20I TGGCCA 2 cut(s) 5, 162
MspI CCGG 5 cut(s) 32, 66, 130, 401, 545
MspR9I CCNGG 1 cut(s) 131
NciI CCSGG 1 cut(s) 131
NcoI CCATGG 1 cut(s) 6
NdeII GATC 3 cut(s) 238, 392, 445
NlaIII CATG 4 cut(s) 10, 73, 291, 567
NlaIV GGNNCC 1 cut(s) 135
NspV TTCGAA 1 cut(s) 500
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 1 cut(s) 472
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PshBI ATTAAT 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 135
PspPI GGNCC 1 cut(s) 316
RsaI GTAC 2 cut(s) 97, 463
RsaNI GTAC 2 cut(s) 96, 462
RseI CAYNNNNRTG 1 cut(s) 142
SaqAI TTAA 3 cut(s) 60, 116, 329
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 3 cut(s) 238, 392, 445
Sau96I GGNCC 1 cut(s) 316
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 1 cut(s) 39
SetI ASST 8 cut(s) 47, 109, 159, 307, 408, 422, 518, 543
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 142
Sse9I AATT 3 cut(s) 326, 330, 457
SsiI CCGC 2 cut(s) 90, 277
SspI AATATT 1 cut(s) 484
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 1 cut(s) 129
StyI CCWWGG 2 cut(s) 6, 163
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 2 cut(s) 47, 159
TaqI TCGA 4 cut(s) 39, 207, 293, 500
TasI AATT 3 cut(s) 326, 330, 457
TatI WGTACW 2 cut(s) 95, 461
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 3 cut(s) 60, 116, 329
Tru9I TTAA 3 cut(s) 60, 116, 329
TscAI CASTG 2 cut(s) 225, 448
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 1 cut(s) 276
TspGWI ACGGA 2 cut(s) 225, 337
TspRI CASTG 2 cut(s) 225, 448
VpaK11BI GGWCC 1 cut(s) 316
VspI ATTAAT 1 cut(s) 329
XspI CTAG 1 cut(s) 390
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.