Rh5DG209800

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003122
Physical Location & Seq
Forward (+)
23235124 .. 23235747
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG209800.1

Sequence Viewer

Length: 624 bp
ATGATGATGTCCCTGAAGAACTTAATTGGAAGCCTGAGCATGATCGGCAGCATATTCATGGCCATGGCAACCATAAACCACACATCTCCGGTGCTCGATACGTCTGGGAAGGCACTTAAATCCGGCATAGATTACTACATCAAACCCGCCAGTACTTACAAAGGTGGTGCTTTAACTCTAATAAACCGGGAGCCATATTTATGTCCACTCTACGTTGGCCAAGGAAATGTCTCGGGCTGGCCTTTTCAGTTCAAGCCATTTTTCGACGGAGAAACAGTGGTGAGGGAGAGTAGGGATCAAATGATTATATCCCGTGTCTCTACACATTGCAGGTGGTTAAATTCATGGTCGATTGAAAAAGCTAACCCAGAGGACCAAGGGAAATTAATTGCTATGCAAGAACCTGCAAGAGGAAAACTGCCAAATGGTAAATACTTTAGGATTACTAGATCACAGCCGGAGGTGGATGGTAAAAGCTACATTCTGAGATGGTGTCCCACCGATATGTGTCCTAATTGTACATTCGCTGATTGTGGAAATATTGGTTCTTTAGATTCGAATGGAGGGAAGAGGTTCTTGGCTTTGAATGGTTCTGCGCTTCCGGTTGTGTTTGAGAGGGCTTAA

Protein Analysis

207

Amino Acids

22.92

Weight (kDa)

9.04

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 31 - 205 2.4e-31 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 321
Acc36I ACCTGC 2 cut(s) 321, 412
AciI CCGC 1 cut(s) 147
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 2 cut(s) 60, 217
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 2 cut(s) 154, 520
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 253, 356, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 2 cut(s) 362, 477
AluI AGCT 2 cut(s) 362, 477
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 235, 322
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 3 cut(s) 60, 217, 239
ApeKI GCWGC 1 cut(s) 48
ApoI RAATTY 1 cut(s) 340
AseI ATTAAT 1 cut(s) 386
Asp700I GAANNNNTTC 1 cut(s) 572
AspLEI GCGC 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 373
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 292
AsuII TTCGAA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 1 cut(s) 373
BalI TGGCCA 2 cut(s) 62, 219
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 461, 483
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 235, 322
BfaI CTAG 1 cut(s) 447
BfuAI ACCTGC 2 cut(s) 321, 412
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 373
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 188
BsaJI CCNNGG 3 cut(s) 63, 220, 376
BsaWI WCCGGW 2 cut(s) 88, 601
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 3 cut(s) 63, 220, 376
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 2 cut(s) 26, 476
BseNI ACTGG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 3 cut(s) 62, 219, 241
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 5 cut(s) 89, 123, 187, 458, 602
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 235, 322
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 3 cut(s) 62, 219, 241
BsoBI CYCGRG 1 cut(s) 232
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 96
Bsp1407I TGTACA 1 cut(s) 518
Bsp143I GATC 3 cut(s) 42, 295, 449
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 1 cut(s) 147
BspANI GGCC 3 cut(s) 62, 219, 241
BspCNI CTCAG 2 cut(s) 27, 477
BspLI GGNNCC 1 cut(s) 192
BspMI ACCTGC 2 cut(s) 321, 412
BspPI GGATC 1 cut(s) 303
BspT104I TTCGAA 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 518
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 3 cut(s) 63, 220, 376
BssMI GATC 3 cut(s) 42, 295, 449
BssT1I CCWWGG 3 cut(s) 63, 220, 376
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 1 cut(s) 239
BstDEI CTNAG 2 cut(s) 35, 485
BstDSI CCRYGG 1 cut(s) 63
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 472
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 3 cut(s) 45, 298, 452
BstMAI GTCTC 2 cut(s) 235, 322
BstMBI GATC 3 cut(s) 42, 295, 449
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 60
BsuRI GGCC 3 cut(s) 62, 219, 241
BtgI CCRYGG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 2 cut(s) 321, 412
Cac8I GCNNGC 1 cut(s) 239
CfoI GCGC 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 373
Csp6I GTAC 2 cut(s) 153, 519
CviAII CATG 4 cut(s) 40, 58, 64, 345
CviQI GTAC 2 cut(s) 153, 519
DdeI CTNAG 2 cut(s) 35, 485
DpnI GATC 3 cut(s) 44, 297, 451
DpnII GATC 3 cut(s) 42, 295, 449
EaeI YGGCCR 2 cut(s) 60, 217
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 3 cut(s) 63, 220, 376
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoT14I CCWWGG 3 cut(s) 63, 220, 376
ErhI CCWWGG 3 cut(s) 63, 220, 376
FaeI CATG 4 cut(s) 43, 61, 67, 348
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 480
FatI CATG 4 cut(s) 39, 57, 63, 344
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 447
GlaI GCGC 1 cut(s) 597
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 3 cut(s) 62, 219, 241
HapII CCGG 5 cut(s) 89, 123, 187, 458, 602
HhaI GCGC 1 cut(s) 598
Hin1II CATG 4 cut(s) 43, 61, 67, 348
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HinfI GANTC 1 cut(s) 554
HpaII CCGG 5 cut(s) 89, 123, 187, 458, 602
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 1 cut(s) 206
Hpy188I TCNGA 1 cut(s) 486
Hpy8I GTNNAC 1 cut(s) 206
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 2 cut(s) 101, 213
HpyCH4V TGCA 3 cut(s) 330, 397, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 2 cut(s) 35, 485
HpySE526I ACGT 2 cut(s) 101, 213
Hsp92II CATG 4 cut(s) 43, 61, 67, 348
HspAI GCGC 1 cut(s) 596
Kzo9I GATC 3 cut(s) 42, 295, 449
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 1 cut(s) 60
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 101, 213
MalI GATC 3 cut(s) 44, 297, 451
MboI GATC 3 cut(s) 42, 295, 449
MboII GAAGA 2 cut(s) 28, 580
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 2 cut(s) 62, 219
MluCI AATT 5 cut(s) 24, 340, 383, 387, 514
MluNI TGGCCA 2 cut(s) 62, 219
MnlI CCTC 7 cut(s) 276, 364, 404, 454, 557, 564, 609
Mox20I TGGCCA 2 cut(s) 62, 219
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 2 cut(s) 62, 219
MseI TTAA 6 cut(s) 23, 117, 173, 338, 386, 622
MslI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
Msp20I TGGCCA 2 cut(s) 62, 219
MspI CCGG 5 cut(s) 89, 123, 187, 458, 602
MspR9I CCNGG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 1 cut(s) 188
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 42, 295, 449
NlaIII CATG 4 cut(s) 43, 61, 67, 348
NlaIV GGNNCC 1 cut(s) 192
NspV TTCGAA 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 554
PkrI GCNGC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 1 cut(s) 373
RsaI GTAC 2 cut(s) 154, 520
RsaNI GTAC 2 cut(s) 153, 519
RseI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
SaqAI TTAA 6 cut(s) 23, 117, 173, 338, 386, 622
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 3 cut(s) 42, 295, 449
Sau96I GGNCC 1 cut(s) 373
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 96
SetI ASST 9 cut(s) 104, 166, 216, 335, 364, 406, 465, 479, 575
SfuI TTCGAA 1 cut(s) 557
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
Sse9I AATT 5 cut(s) 24, 340, 383, 387, 514
SsiI CCGC 1 cut(s) 147
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 3 cut(s) 63, 220, 376
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 2 cut(s) 104, 216
TaqI TCGA 4 cut(s) 96, 264, 350, 557
TasI AATT 5 cut(s) 24, 340, 383, 387, 514
TatI WGTACW 2 cut(s) 152, 518
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 6 cut(s) 23, 117, 173, 338, 386, 622
Tru9I TTAA 6 cut(s) 23, 117, 173, 338, 386, 622
TscAI CASTG 1 cut(s) 282
TseI GCWGC 1 cut(s) 48
TspDTI ATGAA 2 cut(s) 46, 333
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 282
VpaK11BI GGWCC 1 cut(s) 373
VspI ATTAAT 1 cut(s) 386
XagI CCTNNNNNAGG 1 cut(s) 408
XapI RAATTY 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 1 cut(s) 447
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.