RLG00000033138

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003122
Physical Location & Seq
Reverse (-)
23719926 .. 23720525
600 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033138

Sequence Viewer

Length: 600 bp
ATGTCCATGAAGTTGATCGGAAGCATATGGCTAGTAATGGCCATGGCAACCGTAGCCCAAGATAGCGCTCCAGTGCTCGACACCTCTGGGCAGGCTCTTCAATCTGGTGTAGACTACTACATCAAACCTGCCATCACCGACAACGGCGGCCGTTTCACTTTGATCAACCGAACCGGCTTGTGTCCTTTGTATGTTGGTCAAGAAAACACCTCAGGCCCCAAGGGCTACCCGGTGACCTTTGCGCCTTTCGCAGAGGGTGAGACGGTGGTAAGAGTGGGTAGGGATCAGAAGATCACATTCTCTGCATCCACAATCTGTGTGCAATCGACTACATGGAAGGTGGGTGAGACTCAGTCCGAGACCCAAAGGAGATTGATTGTCACCGGAATTGATGAAAACGAAAGCCGGCCTACCGGAAACTACTTCAAGATTAACAAACAGGCAGATCTTGATGGCATTTACAATTTCGAATGGTGCCCTACTGAAGTGTGCCCGATTTGTAGGTTTATCTGCTCAGATGTGGGTGCTTTGGTTGAGAATGACAAGAGGTTGTTGGCCTTGGATGGTTCTGTGCTTCCTGTTGTCTTTGAGAGGGCTTAA

Protein Analysis

200

Amino Acids

21.77

Weight (kDa)

4.94

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 25 - 197 2.5e-48 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 474
AccI GTMKAC 1 cut(s) 111
AciI CCGC 1 cut(s) 147
AclWI GGATC 1 cut(s) 291
AcoI YGGCCR 2 cut(s) 39, 148
AcuI CTGAAG 1 cut(s) 504
AfeI AGCGCT 1 cut(s) 67
AgsI TTSAA 2 cut(s) 101, 427
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 3 cut(s) 254, 341, 353
AlwI GGATC 1 cut(s) 291
Aor51HI AGCGCT 1 cut(s) 67
AoxI GGCC 5 cut(s) 39, 148, 214, 407, 555
AspLEI GCGC 2 cut(s) 68, 244
AspS9I GGNCC 1 cut(s) 215
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 5 cut(s) 127, 244, 269, 356, 373
AsuII TTCGAA 1 cut(s) 468
AxyI CCTNAGG 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 479, 494
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 474
Bbv12I GWGCWC 1 cut(s) 78
BccI CCATC 3 cut(s) 140, 446, 557
BceAI ACGGC 2 cut(s) 135, 160
BclI TGATCA 1 cut(s) 162
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 3 cut(s) 254, 341, 353
BfaI CTAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 136
BglI GCCNNNNNGGC 1 cut(s) 222
BglII AGATCT 1 cut(s) 445
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 2 cut(s) 217, 476
BmrFI CCNGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 353
BsaJI CCNNGG 3 cut(s) 42, 219, 558
BsaWI WCCGGW 2 cut(s) 383, 413
Bse118I RCCGGY 2 cut(s) 173, 405
Bse1I ACTGG 1 cut(s) 71
Bse21I CCTNAGG 1 cut(s) 211
BseDI CCNNGG 3 cut(s) 42, 219, 558
BseGI GGATG 2 cut(s) 305, 568
BseMII CTCAG 3 cut(s) 225, 365, 528
BseNI ACTGG 1 cut(s) 71
BseSI GKGCMC 2 cut(s) 479, 494
BseX3I CGGCCG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 5 cut(s) 41, 150, 216, 409, 557
BshNI GGYRCC 1 cut(s) 474
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 5 cut(s) 174, 230, 384, 406, 414
BsmAI GTCTC 3 cut(s) 254, 341, 353
BsmBI CGTCTC 1 cut(s) 254
BsnI GGCC 5 cut(s) 41, 150, 216, 409, 557
Bso31I GGTCTC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 3 cut(s) 78, 479, 494
Bsp143I GATC 5 cut(s) 15, 162, 283, 291, 445
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 1 cut(s) 147
BspANI GGCC 5 cut(s) 41, 150, 216, 409, 557
BspCNI CTCAG 3 cut(s) 224, 364, 527
BspLI GGNNCC 2 cut(s) 217, 476
BspMI ACCTGC 1 cut(s) 136
BspPI GGATC 1 cut(s) 291
BspQI GCTCTTC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 474
BspTNI GGTCTC 1 cut(s) 353
BsrFI RCCGGY 2 cut(s) 173, 405
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 2 cut(s) 173, 405
BssECI CCNNGG 3 cut(s) 42, 219, 558
BssMI GATC 5 cut(s) 15, 162, 283, 291, 445
BssT1I CCWWGG 3 cut(s) 42, 219, 558
Bst4CI ACNGT 2 cut(s) 52, 265
Bst6I CTCTTC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 2 cut(s) 93, 407
BstDEI CTNAG 3 cut(s) 211, 351, 514
BstDSI CCRYGG 1 cut(s) 42
BstEII GGTNACC 1 cut(s) 232
BstF5I GGATG 2 cut(s) 305, 568
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 244
BstKTI GATC 5 cut(s) 18, 165, 286, 294, 448
BstMAI GTCTC 3 cut(s) 254, 341, 353
BstMBI GATC 5 cut(s) 15, 162, 283, 291, 445
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 3 cut(s) 53, 222, 248
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 1 cut(s) 228
BstSLI GKGCMC 2 cut(s) 479, 494
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BstZI CGGCCG 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 211
BsuRI GGCC 5 cut(s) 41, 150, 216, 409, 557
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 2 cut(s) 305, 568
BtsIMutI CAGTG 1 cut(s) 78
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 2 cut(s) 93, 407
CfoI GCGC 2 cut(s) 68, 244
Cfr10I RCCGGY 2 cut(s) 173, 405
Cfr13I GGNCC 1 cut(s) 215
CviAII CATG 3 cut(s) 7, 43, 333
DdeI CTNAG 3 cut(s) 211, 351, 514
DpnI GATC 5 cut(s) 17, 164, 285, 293, 447
DpnII GATC 5 cut(s) 15, 162, 283, 291, 445
EaeI YGGCCR 2 cut(s) 39, 148
EagI CGGCCG 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 148
Eco130I CCWWGG 3 cut(s) 42, 219, 558
Eco31I GGTCTC 1 cut(s) 353
Eco47III AGCGCT 1 cut(s) 67
Eco52I CGGCCG 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 504
Eco81I CCTNAGG 1 cut(s) 211
Eco91I GGTNACC 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 232
EcoT14I CCWWGG 3 cut(s) 42, 219, 558
ErhI CCWWGG 3 cut(s) 42, 219, 558
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 3 cut(s) 10, 46, 336
FaiI YATR 6 cut(s) 8, 26, 28, 44, 192, 334
FatI CATG 3 cut(s) 6, 42, 332
FauNDI CATATG 1 cut(s) 26
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 148
FokI GGATG 2 cut(s) 292, 575
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 1 cut(s) 32
GlaI GCGC 2 cut(s) 67, 243
GluI GCNGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 5 cut(s) 41, 150, 216, 409, 557
HapII CCGG 5 cut(s) 174, 230, 384, 406, 414
HhaI GCGC 2 cut(s) 68, 244
Hin1II CATG 3 cut(s) 10, 46, 336
Hin6I GCGC 2 cut(s) 66, 242
HinP1I GCGC 2 cut(s) 66, 242
HinfI GANTC 1 cut(s) 349
HpaII CCGG 5 cut(s) 174, 230, 384, 406, 414
HphI GGTGA 5 cut(s) 127, 244, 269, 356, 373
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 4 cut(s) 20, 288, 358, 517
Hpy188III TCNNGA 3 cut(s) 200, 427, 449
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 331
HpyCH4III ACNGT 2 cut(s) 52, 265
HpyCH4V TGCA 2 cut(s) 305, 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 222, 248
HpyF3I CTNAG 3 cut(s) 211, 351, 514
Hsp92II CATG 3 cut(s) 10, 46, 336
HspAI GCGC 2 cut(s) 66, 242
KroI GCCGGC 1 cut(s) 405
KroNI GCCGGC 1 cut(s) 407
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 5 cut(s) 15, 162, 283, 291, 445
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 73
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 32
MaeIII GTNAC 2 cut(s) 232, 379
MalI GATC 5 cut(s) 17, 164, 285, 293, 447
MboI GATC 5 cut(s) 15, 162, 283, 291, 445
MboII GAAGA 2 cut(s) 89, 301
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 78, 479, 494
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 2 cut(s) 387, 463
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 343
MnlI CCTC 5 cut(s) 94, 220, 247, 540, 585
Mox20I TGGCCA 1 cut(s) 41
MroNI GCCGGC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 41
MseI TTAA 2 cut(s) 432, 598
Msp20I TGGCCA 1 cut(s) 41
MspI CCGG 5 cut(s) 174, 230, 384, 406, 414
MspR9I CCNGG 1 cut(s) 230
MwoI GCNNNNNNNGC 3 cut(s) 53, 222, 248
NaeI GCCGGC 1 cut(s) 407
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 26
NdeII GATC 5 cut(s) 15, 162, 283, 291, 445
NgoMIV GCCGGC 1 cut(s) 405
NlaIII CATG 3 cut(s) 10, 46, 336
NlaIV GGNNCC 2 cut(s) 217, 476
NmuCI GTSAC 2 cut(s) 232, 379
NspV TTCGAA 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 407
PflFI GACNNNGTC 1 cut(s) 352
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PspEI GGTNACC 1 cut(s) 232
PspN4I GGNNCC 2 cut(s) 217, 476
PspPI GGNCC 1 cut(s) 215
PsuI RGATCY 1 cut(s) 445
PsyI GACNNNGTC 1 cut(s) 352
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 432, 598
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 5 cut(s) 15, 162, 283, 291, 445
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 3 cut(s) 78, 479, 494
SetI ASST 7 cut(s) 86, 130, 212, 239, 342, 506, 551
SfaNI GCATC 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 468
Sse9I AATT 2 cut(s) 387, 463
SsiI CCGC 1 cut(s) 147
SspMI CTAG 1 cut(s) 32
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 3 cut(s) 42, 219, 558
TaaI ACNGT 2 cut(s) 52, 265
TaqI TCGA 3 cut(s) 78, 326, 468
TasI AATT 2 cut(s) 387, 463
TauI GCSGC 1 cut(s) 150
Tru1I TTAA 2 cut(s) 432, 598
Tru9I TTAA 2 cut(s) 432, 598
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 2 cut(s) 232, 379
Tsp45I GTSAC 2 cut(s) 232, 379
TspDTI ATGAA 2 cut(s) 23, 408
TspRI CASTG 1 cut(s) 78
Tth111I GACNNNGTC 1 cut(s) 352
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.